AlfalfaGEDB | MS.gene44643

M. sativa cultivar XinJiangDaYe / MS.gene44643

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016740 GO:0016746 GO:0016747
PFAM: PF02458.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44643.t1	XP_003621460.2	93.3	476	32	0	1	476	1	476	1.40E-261	911.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44643.t1	Q940Z5	39.8	475	263	15	7	473	8	467	1.2e-85	318.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44643.t1	G7L650	93.3	476	32	0	1	476	1	476	9.8e-262	911.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene50379	MS.gene44643	PPI
MS.gene44643	MS.gene021905	PPI
MS.gene44643	MS.gene000764	PPI
MS.gene44633	MS.gene44643	PPI
MS.gene44643	MS.gene91614	PPI
MS.gene44643	MS.gene90492	PPI
MS.gene44643	MS.gene043350	PPI
MS.gene48585	MS.gene44643	PPI
MS.gene44643	MS.gene56798	PPI
MS.gene011837	MS.gene44643	PPI
MS.gene066634	MS.gene44643	PPI
MS.gene35741	MS.gene44643	PPI
MS.gene043346	MS.gene44643	PPI
MS.gene91599	MS.gene44643	PPI
MS.gene44643	MS.gene87191	PPI
MS.gene44643	MS.gene56796	PPI
MS.gene44643	MS.gene021906	PPI
MS.gene44643	MS.gene44642	PPI
MS.gene91598	MS.gene44643	PPI
MS.gene44643	MS.gene96337	PPI
MS.gene44643	MS.gene87496	PPI
MS.gene44643	MS.gene070019	PPI
MS.gene44643	MS.gene043348	PPI
MS.gene49853	MS.gene44643	PPI
MS.gene44643	MS.gene90493	PPI
MS.gene44643	MS.gene91596	PPI
MS.gene021910	MS.gene44643	PPI
MS.gene44643	MS.gene021914	PPI
MS.gene44643	MS.gene48581	PPI
MS.gene028996	MS.gene44643	PPI
MS.gene44643	MS.gene31540	PPI
MS.gene44643	MS.gene066657	PPI
MS.gene35745	MS.gene44643	PPI
MS.gene011796	MS.gene44643	PPI
MS.gene011801	MS.gene44643	PPI
MS.gene44643	MS.gene56799	PPI
MS.gene87537	MS.gene44643	PPI
MS.gene000764	MS.gene44643	PPI
MS.gene44643	MS.gene44640	PPI
MS.gene48582	MS.gene44643	PPI
MS.gene44643	MS.gene032349	PPI
MS.gene44643	MS.gene069767	PPI
MS.gene013787	MS.gene44643	PPI
MS.gene44643	MS.gene021904	PPI
MS.gene44643	MS.gene000763	PPI
MS.gene021906	MS.gene44643	PPI
MS.gene31542	MS.gene44643	PPI
MS.gene87532	MS.gene44643	PPI
MS.gene99223	MS.gene44643	PPI
MS.gene44643	MS.gene91613	PPI
MS.gene46265	MS.gene44643	PPI
MS.gene063868	MS.gene44643	PPI
MS.gene44643	MS.gene011716	PPI
MS.gene48581	MS.gene44643	PPI
MS.gene44643	MS.gene44633	PPI
MS.gene44643	MS.gene91595	PPI
MS.gene44643	MS.gene90491	PPI
MS.gene44643	MS.gene043352	PPI
MS.gene99221	MS.gene44643	PPI
MS.gene44643	MS.gene50384	PPI
MS.gene44643	MS.gene38977	PPI
MS.gene03202	MS.gene44643	PPI
MS.gene44643	MS.gene021909	PPI
MS.gene44643	MS.gene50355	PPI
MS.gene44643	MS.gene066658	PPI
MS.gene44643	MS.gene91612	PPI
MS.gene44643	MS.gene021917	PPI
MS.gene007831	MS.gene44643	PPI
MS.gene44642	MS.gene44643	PPI
MS.gene91609	MS.gene44643	PPI
MS.gene44643	MS.gene028998	PPI
MS.gene44643	MS.gene064101	PPI
MS.gene44644	MS.gene44643	PPI
MS.gene44643	MS.gene31545	PPI
MS.gene44643	MS.gene021910	PPI
MS.gene99222	MS.gene44643	PPI
MS.gene44643	MS.gene063865	PPI
MS.gene44643	MS.gene50356	PPI
MS.gene60208	MS.gene44643	PPI
MS.gene44643	MS.gene56797	PPI
MS.gene44643	MS.gene44630	PPI
MS.gene44643	MS.gene069422	PPI
MS.gene032349	MS.gene44643	PPI
MS.gene066656	MS.gene44643	PPI
MS.gene066660	MS.gene44643	PPI
MS.gene44643	MS.gene78818	PPI
MS.gene44643	MS.gene28251	PPI
MS.gene44643	MS.gene016532	PPI
MS.gene44643	MS.gene44647	PPI
MS.gene44643	MS.gene066701	PPI
MS.gene44643	MS.gene021915	PPI
MS.gene012360	MS.gene44643	PPI
MS.gene35744	MS.gene44643	PPI
MS.gene44643	MS.gene011799	PPI
MS.gene028999	MS.gene44643	PPI
MS.gene44643	MS.gene002544	PPI
MS.gene44643	MS.gene50420	PPI
MS.gene44643	MS.gene070270	PPI
MS.gene44643	MS.gene50382	PPI
MS.gene44643	MS.gene90496	PPI
MS.gene44643	MS.gene87536	PPI
MS.gene44643	MS.gene99218	PPI
MS.gene073299	MS.gene44643	PPI
MS.gene44643	MS.gene44463	PPI
MS.gene91597	MS.gene44643	PPI
MS.gene021916	MS.gene44643	PPI
MS.gene32261	MS.gene44643	PPI
MS.gene31546	MS.gene44643	PPI
MS.gene44643	MS.gene48584	PPI
MS.gene99219	MS.gene44643	PPI
MS.gene44643	MS.gene004218	PPI
MS.gene56795	MS.gene44643	PPI
MS.gene44643	MS.gene016477	PPI
MS.gene44643	MS.gene021913	PPI
MS.gene066657	MS.gene44643	PPI
MS.gene44643	MS.gene91611	PPI
MS.gene44643	MS.gene02573	PPI
MS.gene44643	MS.gene011798	PPI
MS.gene069423	MS.gene44643	PPI
MS.gene44643	MS.gene35740	PPI
MS.gene44643	MS.gene028994	PPI
MS.gene44643	MS.gene87577	PPI
MS.gene91615	MS.gene44643	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44643.t1	MTR_7g014160	93.277	476	32	0	1	476	1	476	0.0	922
MS.gene44643.t1	MTR_7g014610	75.368	475	114	3	5	476	4	478	0.0	724
MS.gene44643.t1	MTR_7g014200	73.278	479	117	5	1	476	1	471	0.0	723
MS.gene44643.t1	MTR_7g014620	67.387	463	119	4	17	476	2	435	0.0	600
MS.gene44643.t1	MTR_7g013850	57.764	483	188	9	6	476	5	483	0.0	531
MS.gene44643.t1	MTR_7g014630	79.264	299	60	2	1	297	1	299	3.87e-170	483
MS.gene44643.t1	MTR_7g014230	42.737	475	238	15	10	472	5	457	3.19e-111	337
MS.gene44643.t1	MTR_6g015815	41.026	468	243	10	12	473	20	460	1.55e-109	332
MS.gene44643.t1	MTR_7g014670	82.703	185	32	0	292	476	78	262	4.64e-109	324
MS.gene44643.t1	MTR_7g014330	41.053	475	250	13	10	474	3	457	4.76e-109	331
MS.gene44643.t1	MTR_6g015320	41.314	472	245	13	9	473	8	454	3.79e-108	328
MS.gene44643.t1	MTR_6g015850	40.086	464	244	11	15	473	23	457	8.63e-107	325
MS.gene44643.t1	MTR_7g014250	41.176	476	246	15	10	473	3	456	2.75e-104	319
MS.gene44643.t1	MTR_6g015315	40.773	466	255	9	10	473	6	452	5.07e-104	318
MS.gene44643.t1	MTR_6g015830	39.869	459	237	14	26	473	30	460	6.14e-103	315
MS.gene44643.t1	MTR_7g014180	74.857	175	42	1	1	175	1	173	3.01e-87	265
MS.gene44643.t1	MTR_3g028210	35.924	476	275	10	9	473	8	464	3.35e-83	265
MS.gene44643.t1	MTR_7g014360	37.065	402	207	11	80	473	23	386	7.30e-76	243
MS.gene44643.t1	MTR_2g089765	32.720	489	283	14	9	473	16	482	1.30e-69	230
MS.gene44643.t1	MTR_3g087640	32.784	485	283	16	10	473	10	472	3.06e-65	218
MS.gene44643.t1	MTR_7g015860	31.106	479	265	12	6	456	2	443	5.24e-64	214
MS.gene44643.t1	MTR_2g089650	32.860	493	281	17	1	473	1	463	1.85e-63	213
MS.gene44643.t1	MTR_2g089735	33.333	444	262	13	43	473	39	461	2.39e-63	213
MS.gene44643.t1	MTR_2g089670	31.602	462	277	13	26	473	41	477	3.52e-61	207
MS.gene44643.t1	MTR_2g089755	32.543	464	268	15	30	473	37	475	1.28e-60	206
MS.gene44643.t1	MTR_8g012750	30.321	498	293	15	9	475	3	477	1.08e-58	201
MS.gene44643.t1	MTR_6g015310	33.419	389	190	11	65	451	12	333	9.00e-58	194
MS.gene44643.t1	MTR_7g014310	29.683	347	174	9	115	458	29	308	4.91e-38	141
MS.gene44643.t1	MTR_2g089665	28.035	453	196	15	9	450	2	335	2.09e-36	137
MS.gene44643.t1	MTR_3g435300	31.818	286	160	9	10	279	4	270	1.59e-34	130
MS.gene44643.t1	MTR_3g026950	25.386	453	261	20	55	469	34	447	9.60e-22	97.8
MS.gene44643.t1	MTR_7g086000	25.051	487	312	18	8	473	3	457	1.58e-21	97.4
MS.gene44643.t1	MTR_6g045553	26.419	458	250	18	8	425	13	423	5.42e-20	92.8
MS.gene44643.t1	MTR_8g075610	24.027	437	275	16	52	471	23	419	7.98e-20	92.0
MS.gene44643.t1	MTR_4g007540	24.033	491	284	21	9	471	3	432	1.83e-18	87.8
MS.gene44643.t1	MTR_4g127650	24.421	475	305	16	17	473	18	456	6.92e-18	86.3
MS.gene44643.t1	MTR_4g094828	22.422	446	289	14	26	455	25	429	1.69e-17	85.1
MS.gene44643.t1	MTR_8g079950	24.615	455	283	16	12	457	8	411	2.81e-17	84.3
MS.gene44643.t1	MTR_3g049150	25.629	437	257	18	65	473	51	447	5.55e-17	83.6
MS.gene44643.t1	MTR_4g097090	23.952	501	308	18	1	473	1	456	6.50e-17	83.2
MS.gene44643.t1	MTR_7g085930	23.185	496	314	17	7	473	4	461	7.22e-16	80.1
MS.gene44643.t1	MTR_3g098980	24.940	417	251	16	68	470	65	433	8.48e-16	79.7
MS.gene44643.t1	MTR_7g014210	38.889	108	63	3	330	435	24	130	1.17e-15	75.1
MS.gene44643.t1	MTR_8g079970	22.646	446	289	14	12	449	8	405	3.64e-15	77.8
MS.gene44643.t1	MTR_4g022290	22.297	444	278	14	30	455	30	424	5.64e-15	77.4
MS.gene44643.t1	MTR_8g096530	23.953	430	268	16	5	425	3	382	6.57e-15	77.0
MS.gene44643.t1	MTR_4g094842	26.570	414	229	17	69	455	62	427	1.10e-14	76.3
MS.gene44643.t1	MTR_4g022670	26.166	386	216	15	69	429	55	396	1.44e-14	75.9
MS.gene44643.t1	MTR_3g026310	22.996	474	286	17	9	453	4	427	1.49e-14	75.9
MS.gene44643.t1	MTR_7g014205	35.088	114	61	3	323	435	13	114	2.39e-14	69.7
MS.gene44643.t1	MTR_7g027175	23.150	419	255	16	68	469	67	435	3.99e-14	74.7
MS.gene44643.t1	MTR_8g041770	24.882	422	256	16	27	435	27	400	6.59e-14	73.9
MS.gene44643.t1	MTR_7g084910	25.926	351	196	16	9	344	17	318	3.76e-13	71.6
MS.gene44643.t1	MTR_4g022720	25.060	415	227	17	69	457	55	411	6.69e-13	70.9
MS.gene44643.t1	MTR_3g101960	23.502	434	278	14	16	435	15	408	2.05e-12	69.3
MS.gene44643.t1	MTR_4g094832	25.786	318	183	14	147	453	134	409	2.07e-12	69.3
MS.gene44643.t1	MTR_1g053315	23.410	393	247	18	46	425	58	409	2.22e-11	66.2
MS.gene44643.t1	MTR_7g014340	50.704	71	29	2	10	77	3	70	4.54e-11	59.7
MS.gene44643.t1	MTR_3g102020	24.654	434	268	19	19	435	18	409	5.76e-11	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44643.t1	AT5G39080	40.086	464	256	15	7	463	3	451	1.99e-99	306
MS.gene44643.t1	AT5G39050	39.873	474	264	14	7	473	8	467	6.09e-96	298
MS.gene44643.t1	AT3G29590	39.331	478	250	13	4	473	3	448	1.65e-95	296
MS.gene44643.t1	AT3G29670	38.616	448	246	9	29	473	28	449	5.11e-91	284
MS.gene44643.t1	AT5G39090	38.344	459	247	15	21	473	19	447	4.83e-89	279
MS.gene44643.t1	AT3G29635	36.384	448	261	10	29	473	28	454	2.05e-86	273
MS.gene44643.t1	AT3G29680	33.408	449	274	8	29	475	24	449	1.79e-76	246
MS.gene44643.t1	AT5G61160	30.472	466	297	10	9	469	3	446	3.12e-72	236
MS.gene44643.t1	AT1G03940	31.818	484	282	15	10	473	9	464	4.99e-62	209
MS.gene44643.t1	AT1G03495	32.961	358	201	13	11	354	10	342	8.66e-41	149
MS.gene44643.t1	AT5G23940	26.638	473	291	20	9	462	3	438	8.17e-23	101
MS.gene44643.t1	AT3G29720	30.952	210	121	6	106	310	26	216	1.79e-19	87.4
MS.gene44643.t1	AT5G41040	25.538	372	222	16	66	425	82	410	6.74e-18	86.3
MS.gene44643.t1	AT5G41040	25.538	372	222	16	66	425	66	394	7.58e-18	86.3
MS.gene44643.t1	AT5G48930	23.690	477	303	20	9	471	3	432	1.10e-17	85.5
MS.gene44643.t1	AT3G29690	28.402	169	80	4	323	473	33	178	3.07e-16	77.0
MS.gene44643.t1	AT5G57840	22.736	497	297	20	9	476	3	441	1.14e-14	76.3
MS.gene44643.t1	AT1G65450	24.706	340	197	12	151	474	154	450	1.40e-12	70.1
MS.gene44643.t1	AT3G48720	23.324	373	225	16	68	426	60	385	1.67e-12	69.7

Find 86 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTACTTACTTTGACATATTT+TGG	0.126266	7.3:-83447894	MS.gene44643:CDS
ACAAAAGATGATAAAGATTT+TGG	0.133685	7.3:+83447115	None:intergenic
CCATTACCAAATAATTATTT+TGG	0.140939	7.3:-83446977	MS.gene44643:CDS
TTTGGAGTATATTGGATTAT+TGG	0.190059	7.3:+83447697	None:intergenic
CAATTTCCAAAATAATTATT+TGG	0.203936	7.3:+83446971	None:intergenic
CTCCATCATTTGGAGTATAT+TGG	0.207093	7.3:+83447689	None:intergenic
GTGAAAAGTTCACAAATAAT+TGG	0.216402	7.3:-83447330	MS.gene44643:CDS
GAAGGGTGTGAATGTGAATT+TGG	0.246248	7.3:+83447829	None:intergenic
GAGAGTGAAGATTTGAGAAT+TGG	0.258335	7.3:+83447784	None:intergenic
GAAAAGGAGAAAGAGAAGTT+TGG	0.285622	7.3:-83447040	MS.gene44643:CDS
CGAACATAGTCCTCGAGTTT+TGG	0.302201	7.3:+83447235	None:intergenic
TGCCTATTCGCTTGTTTGTT+TGG	0.316376	7.3:-83447083	MS.gene44643:CDS
CAAGCTCACACGTGAAGATT+TGG	0.321812	7.3:-83447203	MS.gene44643:CDS
GGTGTTTATGAGATTGATTT+TGG	0.325995	7.3:-83446743	MS.gene44643:CDS
TTTCGTTTCCTTGTTCATTT+GGG	0.333723	7.3:+83447284	None:intergenic
CCAAAATAATTATTTGGTAA+TGG	0.352680	7.3:+83446977	None:intergenic
TTTAGAACTTTGTTTGTTGA+AGG	0.360118	7.3:-83446590	MS.gene44643:CDS
CTTTAGCTGGTAACATCGTT+TGG	0.362068	7.3:-83447738	MS.gene44643:CDS
AGCAAATGGTGTTATCGAAA+TGG	0.363374	7.3:-83447171	MS.gene44643:CDS
ATCAAGGAAACTCCATCATT+TGG	0.368212	7.3:+83447679	None:intergenic
ACTCATCATGCTGTTCTTGA+TGG	0.374958	7.3:-83447487	MS.gene44643:CDS
AATGAAAAGGGTGTTCTTGA+TGG	0.383812	7.3:-83446848	MS.gene44643:CDS
GTTGCTGGGTCTAATAGATT+TGG	0.400903	7.3:-83446764	MS.gene44643:CDS
AGTAAAGGTGGAATTGAAGT+TGG	0.405338	7.3:-83446644	MS.gene44643:CDS
TTTAACGTTGTTGTTGTTGT+TGG	0.405998	7.3:+83447975	None:intergenic
AAAAGGAGAAAGAGAAGTTT+GGG	0.406901	7.3:-83447039	MS.gene44643:CDS
GAAACTATCTCCACCTTTGT+TGG	0.407590	7.3:+83446710	None:intergenic
AACCATGTTCATCAAAGCTT+GGG	0.411745	7.3:-83447455	MS.gene44643:CDS
AATAAACATGATAAATGAAA+AGG	0.414449	7.3:-83446861	MS.gene44643:CDS
TAGAGGTTTAACCATTGCTT+TGG	0.417032	7.3:-83446681	MS.gene44643:CDS
AATATGTCAAAGTAAGTAAG+AGG	0.422390	7.3:+83447898	None:intergenic
TTTGAAATGATGGGAGTTGC+TGG	0.422760	7.3:-83446779	MS.gene44643:CDS
AACGAGACAAAGAAGCTTCA+TGG	0.423717	7.3:+83447608	None:intergenic
ATGATTTCCCTGTCTAATAG+AGG	0.429761	7.3:+83447370	None:intergenic
AGGTTTAACCATTGCTTTGG+CGG	0.432846	7.3:-83446678	MS.gene44643:CDS
TGTTTGGCAAAAGCAATTCA+TGG	0.435306	7.3:-83447067	MS.gene44643:CDS
GCAAATGGTGTTATCGAAAT+GGG	0.438928	7.3:-83447170	MS.gene44643:CDS
CAACCATGTTCATCAAAGCT+TGG	0.444772	7.3:-83447456	MS.gene44643:CDS
CAAGACTTTCTCCCTTTAGC+TGG	0.445546	7.3:-83447751	MS.gene44643:CDS
ATTGGTTGTTGATATTCTAA+AGG	0.447010	7.3:+83447715	None:intergenic
TGCCAAACAAACAAGCGAAT+AGG	0.447920	7.3:+83447081	None:intergenic
ATCCAATATACTCCAAATGA+TGG	0.454687	7.3:-83447691	MS.gene44643:CDS
GAGATAGTTTCTGCGGATAG+AGG	0.456278	7.3:-83446698	MS.gene44643:CDS
AGCAATTCATGGAGTTGAAA+AGG	0.457057	7.3:-83447056	MS.gene44643:CDS
GTAAAGGTGGAATTGAAGTT+GGG	0.458078	7.3:-83446643	MS.gene44643:CDS
TTTGGATAAAATAAAGCAAA+TGG	0.463119	7.3:-83447185	MS.gene44643:CDS
TCTTGTTGATACAAAACCAT+TGG	0.464537	7.3:-83446936	MS.gene44643:CDS
AGTTAAAACCTCTATTAGAC+AGG	0.472806	7.3:-83447378	MS.gene44643:CDS
AAGGGTGTGAATGTGAATTT+GGG	0.480626	7.3:+83447830	None:intergenic
ATAAACATGATAAATGAAAA+GGG	0.491218	7.3:-83446860	MS.gene44643:CDS
CAAACGATGTTACCAGCTAA+AGG	0.503485	7.3:+83447739	None:intergenic
AGCTTTGATGAACATGGTTG+AGG	0.508130	7.3:+83447459	None:intergenic
AGCTTCTTGAAGAAGAAAGA+AGG	0.508191	7.3:+83447811	None:intergenic
CAGCTAAAGGGAGAAAGTCT+TGG	0.511543	7.3:+83447752	None:intergenic
AAATAATTATTTGGTAATGG+TGG	0.512227	7.3:+83446980	None:intergenic
TTTAACTCTGGCAACAAAGT+TGG	0.512683	7.3:+83447394	None:intergenic
AAACGATGTTACCAGCTAAA+GGG	0.515290	7.3:+83447740	None:intergenic
TTGTTCATTTGGGAACATCA+TGG	0.519208	7.3:+83447294	None:intergenic
ATTAGACTCTGCAATGATCA+AGG	0.522107	7.3:+83447663	None:intergenic
TTGGATTACATAAACGAAGA+TGG	0.525487	7.3:-83446917	MS.gene44643:CDS
ACGAGACAAAGAAGCTTCAT+GGG	0.525539	7.3:+83447609	None:intergenic
TCTTCGTTTATGTAATCCAA+TGG	0.526428	7.3:+83446920	None:intergenic
ATCATCAAAGACAACGGACT+TGG	0.535734	7.3:-83447352	MS.gene44643:CDS
TAACCTTGCTCTGCAATCTA+CGG	0.539940	7.3:+83447006	None:intergenic
CTAAAGGGAGAAAGTCTTGG+AGG	0.549374	7.3:+83447755	None:intergenic
TTTGCTCTCCGCCAAAGCAA+TGG	0.572654	7.3:+83446670	None:intergenic
ATATGTCAAAGTAAGTAAGA+GGG	0.583236	7.3:+83447899	None:intergenic
ATGATGAGTGCTTATTCCAA+TGG	0.600520	7.3:+83447501	None:intergenic
GCGGAGAGCAAAGATAGTAA+AGG	0.602811	7.3:-83446659	MS.gene44643:CDS
GAATCTGTCGATTCTAAGTG+TGG	0.604551	7.3:+83447577	None:intergenic
AGCCCAAGCTTTGATGAACA+TGG	0.605776	7.3:+83447453	None:intergenic
TATGTCAAAGTAAGTAAGAG+GGG	0.605853	7.3:+83447900	None:intergenic
GCTTCTTGAAGAAGAAAGAA+GGG	0.606219	7.3:+83447812	None:intergenic
TCGCCGTAGATTGCAGAGCA+AGG	0.607885	7.3:-83447009	MS.gene44643:CDS
TGGTTGGGGAAGACCAACAA+AGG	0.614189	7.3:-83446723	MS.gene44643:CDS
ATGATGTTCCCAAATGAACA+AGG	0.619440	7.3:-83447292	MS.gene44643:CDS
GGTGTGAATGTGAATTTGGG+AGG	0.625969	7.3:+83447833	None:intergenic
TTGAAATGATGGGAGTTGCT+GGG	0.626589	7.3:-83446778	MS.gene44643:CDS
GAGAGCAAAGATAGTAAAGG+TGG	0.637310	7.3:-83446656	MS.gene44643:CDS
TTGGGGAAGACCAACAAAGG+TGG	0.638548	7.3:-83446720	MS.gene44643:CDS
TAAAGGGAGAAAGTCTTGGA+GGG	0.642807	7.3:+83447756	None:intergenic
GTGTGAATGTGAATTTGGGA+GGG	0.652561	7.3:+83447834	None:intergenic
AAAGGTGGAGATAGTTTCTG+CGG	0.670530	7.3:-83446705	MS.gene44643:CDS
GTTAAAACCTCTATTAGACA+GGG	0.687259	7.3:-83447377	MS.gene44643:CDS
AGGGAAATCATCAAAGACAA+CGG	0.689246	7.3:-83447358	MS.gene44643:CDS
CGAGACAAAGAAGCTTCATG+GGG	0.726125	7.3:+83447610	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATAAACATGATAAATGAAA+AGG	-	chr7.3:83447695-83447714	MS.gene44643:CDS	15.0%
!!	ATAAACATGATAAATGAAAA+GGG	-	chr7.3:83447696-83447715	MS.gene44643:CDS	15.0%
!!	CAATTTCCAAAATAATTATT+TGG	+	chr7.3:83447588-83447607	None:intergenic	15.0%
!!	CCAAAATAATTATTTGGTAA+TGG	+	chr7.3:83447582-83447601	None:intergenic	20.0%
!!	TTTGGATAAAATAAAGCAAA+TGG	-	chr7.3:83447371-83447390	MS.gene44643:CDS	20.0%
!!!	AAATAATTATTTGGTAATGG+TGG	+	chr7.3:83447579-83447598	None:intergenic	20.0%
!!!	AAGTGAAAAATTTGAAATGA+TGG	-	chr7.3:83447767-83447786	MS.gene44643:CDS	20.0%
!!!	ACAAAAGATGATAAAGATTT+TGG	+	chr7.3:83447444-83447463	None:intergenic	20.0%
!!!	AGTGAAAAATTTGAAATGAT+GGG	-	chr7.3:83447768-83447787	MS.gene44643:CDS	20.0%
!!!	ATTATTTTGGAAATTGTGTA+TGG	-	chr7.3:83447592-83447611	MS.gene44643:CDS	20.0%
!!!	CCATTACCAAATAATTATTT+TGG	-	chr7.3:83447579-83447598	MS.gene44643:CDS	20.0%
!!!	TTACTTACTTTGACATATTT+TGG	-	chr7.3:83446662-83446681	MS.gene44643:CDS	20.0%
!!!	TTATTTTGGAAATTGTGTAT+GGG	-	chr7.3:83447593-83447612	MS.gene44643:CDS	20.0%
!	AATATGTCAAAGTAAGTAAG+AGG	+	chr7.3:83446661-83446680	None:intergenic	25.0%
!	ATATGTCAAAGTAAGTAAGA+GGG	+	chr7.3:83446660-83446679	None:intergenic	25.0%
!	ATTGGTTGTTGATATTCTAA+AGG	+	chr7.3:83446844-83446863	None:intergenic	25.0%
!	GTGAAAAGTTCACAAATAAT+TGG	-	chr7.3:83447226-83447245	MS.gene44643:CDS	25.0%
!!	TTTAGAACTTTGTTTGTTGA+AGG	-	chr7.3:83447966-83447985	MS.gene44643:CDS	25.0%
!!	TTTGGAGTATATTGGATTAT+TGG	+	chr7.3:83446862-83446881	None:intergenic	25.0%
!!!	TATTTTGGAAATTGTGTATG+GGG	-	chr7.3:83447594-83447613	MS.gene44643:CDS	25.0%
!!!	TTATGAGATTGATTTTGGTT+GGG	-	chr7.3:83447818-83447837	MS.gene44643:CDS	25.0%
!!!	TTTATGAGATTGATTTTGGT+TGG	-	chr7.3:83447817-83447836	MS.gene44643:CDS	25.0%
	AAAAGGAGAAAGAGAAGTTT+GGG	-	chr7.3:83447517-83447536	MS.gene44643:CDS	30.0%
	AGTTAAAACCTCTATTAGAC+AGG	-	chr7.3:83447178-83447197	MS.gene44643:CDS	30.0%
	ATCCAATATACTCCAAATGA+TGG	-	chr7.3:83446865-83446884	MS.gene44643:CDS	30.0%
	GTTAAAACCTCTATTAGACA+GGG	-	chr7.3:83447179-83447198	MS.gene44643:CDS	30.0%
	TAGAAAAAAACACGTTCTAC+TGG	+	chr7.3:83446700-83446719	None:intergenic	30.0%
	TATGTCAAAGTAAGTAAGAG+GGG	+	chr7.3:83446659-83446678	None:intergenic	30.0%
	TCTTCGTTTATGTAATCCAA+TGG	+	chr7.3:83447639-83447658	None:intergenic	30.0%
	TCTTGTTGATACAAAACCAT+TGG	-	chr7.3:83447620-83447639	MS.gene44643:CDS	30.0%
	TTGGATTACATAAACGAAGA+TGG	-	chr7.3:83447639-83447658	MS.gene44643:CDS	30.0%
	TTTCGTTTCCTTGTTCATTT+GGG	+	chr7.3:83447275-83447294	None:intergenic	30.0%
!	AAAAACCACTTTCTGGAAAA+AGG	+	chr7.3:83447035-83447054	None:intergenic	30.0%
!	AAAACCACTTTCTGGAAAAA+GGG	+	chr7.3:83447034-83447053	None:intergenic	30.0%
!	TTTAACGTTGTTGTTGTTGT+TGG	+	chr7.3:83446584-83446603	None:intergenic	30.0%
!	TTTTCGTTTCCTTGTTCATT+TGG	+	chr7.3:83447276-83447295	None:intergenic	30.0%
!!	GGTGTTTATGAGATTGATTT+TGG	-	chr7.3:83447813-83447832	MS.gene44643:CDS	30.0%
!!!	ACTTTGACATATTTTGGCTA+AGG	-	chr7.3:83446668-83446687	MS.gene44643:CDS	30.0%
!!!	TATGAGATTGATTTTGGTTG+GGG	-	chr7.3:83447819-83447838	MS.gene44643:CDS	30.0%
!!!	TCTAATAGAGGTTTTAACTC+TGG	+	chr7.3:83447177-83447196	None:intergenic	30.0%
	AAAAAACACGTTCTACTGGA+TGG	+	chr7.3:83446696-83446715	None:intergenic	35.0%
	AAACGATGTTACCAGCTAAA+GGG	+	chr7.3:83446819-83446838	None:intergenic	35.0%
	AACCATGTTCATCAAAGCTT+GGG	-	chr7.3:83447101-83447120	MS.gene44643:CDS	35.0%
	AGCAATTCATGGAGTTGAAA+AGG	-	chr7.3:83447500-83447519	MS.gene44643:CDS	35.0%
	AGCTTCTTGAAGAAGAAAGA+AGG	+	chr7.3:83446748-83446767	None:intergenic	35.0%
	AGGGAAATCATCAAAGACAA+CGG	-	chr7.3:83447198-83447217	MS.gene44643:CDS	35.0%
	AGTAAAGGTGGAATTGAAGT+TGG	-	chr7.3:83447912-83447931	MS.gene44643:CDS	35.0%
	ATCAAGGAAACTCCATCATT+TGG	+	chr7.3:83446880-83446899	None:intergenic	35.0%
	ATGATGAGTGCTTATTCCAA+TGG	+	chr7.3:83447058-83447077	None:intergenic	35.0%
	ATGATGTTCCCAAATGAACA+AGG	-	chr7.3:83447264-83447283	MS.gene44643:CDS	35.0%
	ATGATTTCCCTGTCTAATAG+AGG	+	chr7.3:83447189-83447208	None:intergenic	35.0%
	ATTAGACTCTGCAATGATCA+AGG	+	chr7.3:83446896-83446915	None:intergenic	35.0%
	GAAAAGGAGAAAGAGAAGTT+TGG	-	chr7.3:83447516-83447535	MS.gene44643:CDS	35.0%
	GAGAGTGAAGATTTGAGAAT+TGG	+	chr7.3:83446775-83446794	None:intergenic	35.0%
	GCTTCTTGAAGAAGAAAGAA+GGG	+	chr7.3:83446747-83446766	None:intergenic	35.0%
	GTAAAGGTGGAATTGAAGTT+GGG	-	chr7.3:83447913-83447932	MS.gene44643:CDS	35.0%
	TAGAGGTTTAACCATTGCTT+TGG	-	chr7.3:83447875-83447894	MS.gene44643:CDS	35.0%
	TGTTTGGCAAAAGCAATTCA+TGG	-	chr7.3:83447489-83447508	MS.gene44643:CDS	35.0%
	TTGTTCATTTGGGAACATCA+TGG	+	chr7.3:83447265-83447284	None:intergenic	35.0%
	TTTAACTCTGGCAACAAAGT+TGG	+	chr7.3:83447165-83447184	None:intergenic	35.0%
!	AAGGGTGTGAATGTGAATTT+GGG	+	chr7.3:83446729-83446748	None:intergenic	35.0%
!	ATAACCCTTTTTCCAGAAAG+TGG	-	chr7.3:83447027-83447046	MS.gene44643:CDS	35.0%
!	CTCCATCATTTGGAGTATAT+TGG	+	chr7.3:83446870-83446889	None:intergenic	35.0%
!	GAAAAACTTTGGTGTGTTCA+TGG	+	chr7.3:83446609-83446628	None:intergenic	35.0%
!	TTTCTGGAAAAAGGGTTATC+TGG	+	chr7.3:83447026-83447045	None:intergenic	35.0%
!!	AATGAAAAGGGTGTTCTTGA+TGG	-	chr7.3:83447708-83447727	MS.gene44643:CDS	35.0%
!!	AGCAAATGGTGTTATCGAAA+TGG	-	chr7.3:83447385-83447404	MS.gene44643:CDS	35.0%
!!	GCAAATGGTGTTATCGAAAT+GGG	-	chr7.3:83447386-83447405	MS.gene44643:CDS	35.0%
!!	TGATGATGGAGGAAAAACTT+TGG	+	chr7.3:83446620-83446639	None:intergenic	35.0%
!!!	GTTGTTTTTCGAGTTGATGA+TGG	+	chr7.3:83446634-83446653	None:intergenic	35.0%
	AAAGGTGGAGATAGTTTCTG+CGG	-	chr7.3:83447851-83447870	MS.gene44643:CDS	40.0%
	AACGAGACAAAGAAGCTTCA+TGG	+	chr7.3:83446951-83446970	None:intergenic	40.0%
	ACGAGACAAAGAAGCTTCAT+GGG	+	chr7.3:83446950-83446969	None:intergenic	40.0%
	ACTCATCATGCTGTTCTTGA+TGG	-	chr7.3:83447069-83447088	MS.gene44643:CDS	40.0%
	AGGTTTAACCATTGCTTTGG+CGG	-	chr7.3:83447878-83447897	MS.gene44643:CDS	40.0%
	CAAACGATGTTACCAGCTAA+AGG	+	chr7.3:83446820-83446839	None:intergenic	40.0%
	CAACCATGTTCATCAAAGCT+TGG	-	chr7.3:83447100-83447119	MS.gene44643:CDS	40.0%
	CCAATGGAAAAACCACTTTC+TGG	+	chr7.3:83447042-83447061	None:intergenic	40.0%
	CTTTAGCTGGTAACATCGTT+TGG	-	chr7.3:83446818-83446837	MS.gene44643:CDS	40.0%
	GAAACTATCTCCACCTTTGT+TGG	+	chr7.3:83447849-83447868	None:intergenic	40.0%
	GAGAGCAAAGATAGTAAAGG+TGG	-	chr7.3:83447900-83447919	MS.gene44643:CDS	40.0%
	GTGTGAATGTGAATTTGGGA+GGG	+	chr7.3:83446725-83446744	None:intergenic	40.0%
	GTTGCTGGGTCTAATAGATT+TGG	-	chr7.3:83447792-83447811	MS.gene44643:CDS	40.0%
	TAAAGGGAGAAAGTCTTGGA+GGG	+	chr7.3:83446803-83446822	None:intergenic	40.0%
	TAACCTTGCTCTGCAATCTA+CGG	+	chr7.3:83447553-83447572	None:intergenic	40.0%
	TGCCAAACAAACAAGCGAAT+AGG	+	chr7.3:83447478-83447497	None:intergenic	40.0%
	TGCCTATTCGCTTGTTTGTT+TGG	-	chr7.3:83447473-83447492	MS.gene44643:CDS	40.0%
!	ACCTTTTGAACCAAAACTCG+AGG	-	chr7.3:83447311-83447330	MS.gene44643:CDS	40.0%
!	AGCTTTGATGAACATGGTTG+AGG	+	chr7.3:83447100-83447119	None:intergenic	40.0%
!	ATCATCAAAGACAACGGACT+TGG	-	chr7.3:83447204-83447223	MS.gene44643:CDS	40.0%
!	CCAGAAAGTGGTTTTTCCAT+TGG	-	chr7.3:83447039-83447058	MS.gene44643:CDS	40.0%
!	GAAGGGTGTGAATGTGAATT+TGG	+	chr7.3:83446730-83446749	None:intergenic	40.0%
!	TTGAAATGATGGGAGTTGCT+GGG	-	chr7.3:83447778-83447797	MS.gene44643:CDS	40.0%
!	TTTGAAATGATGGGAGTTGC+TGG	-	chr7.3:83447777-83447796	MS.gene44643:CDS	40.0%
!!	GAATCTGTCGATTCTAAGTG+TGG	+	chr7.3:83446982-83447001	None:intergenic	40.0%
!!!	GTTTTTCGAGTTGATGATGG+AGG	+	chr7.3:83446631-83446650	None:intergenic	40.0%
!!!	TCCTCGAGTTTTGGTTCAAA+AGG	+	chr7.3:83447315-83447334	None:intergenic	40.0%
	CAAGACTTTCTCCCTTTAGC+TGG	-	chr7.3:83446805-83446824	MS.gene44643:CDS	45.0%
	CAAGCTCACACGTGAAGATT+TGG	-	chr7.3:83447353-83447372	MS.gene44643:CDS	45.0%
	CAGCTAAAGGGAGAAAGTCT+TGG	+	chr7.3:83446807-83446826	None:intergenic	45.0%
	CGAGACAAAGAAGCTTCATG+GGG	+	chr7.3:83446949-83446968	None:intergenic	45.0%
	CTAAAGGGAGAAAGTCTTGG+AGG	+	chr7.3:83446804-83446823	None:intergenic	45.0%
	GAGATAGTTTCTGCGGATAG+AGG	-	chr7.3:83447858-83447877	MS.gene44643:CDS	45.0%
	GCGGAGAGCAAAGATAGTAA+AGG	-	chr7.3:83447897-83447916	MS.gene44643:CDS	45.0%
	GGTGTGAATGTGAATTTGGG+AGG	+	chr7.3:83446726-83446745	None:intergenic	45.0%
!	AGCCCAAGCTTTGATGAACA+TGG	+	chr7.3:83447106-83447125	None:intergenic	45.0%
!	CGAACATAGTCCTCGAGTTT+TGG	+	chr7.3:83447324-83447343	None:intergenic	45.0%
	TGGTTGGGGAAGACCAACAA+AGG	-	chr7.3:83447833-83447852	MS.gene44643:CDS	50.0%
	TTGGGGAAGACCAACAAAGG+TGG	-	chr7.3:83447836-83447855	MS.gene44643:CDS	50.0%
	TTTGCTCTCCGCCAAAGCAA+TGG	+	chr7.3:83447889-83447908	None:intergenic	50.0%
	TCGCCGTAGATTGCAGAGCA+AGG	-	chr7.3:83447547-83447566	MS.gene44643:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	83446574	83448004	83446574	ID=MS.gene44643
chr7.3	mRNA	83446574	83448004	83446574	ID=MS.gene44643.t1;Parent=MS.gene44643
chr7.3	exon	83446574	83448004	83446574	ID=MS.gene44643.t1.exon1;Parent=MS.gene44643.t1
chr7.3	CDS	83446574	83448004	83446574	ID=cds.MS.gene44643.t1;Parent=MS.gene44643.t1

Gene Sequence

>MS.gene44643

ATGGCTTCCAACAACAACAACAACGTTAAAATCCATGAACACACCAAAGTTTTTCCTCCATCATCAACTCGAAAAACAACAACCCCTCTTACTTACTTTGACATATTTTGGCTAAGGTTCCATCCAGTAGAACGTGTTTTTTTCTACACCCTCCCAAATTCACATTCACACCCTTCTTTCTTCTTCAAGAAGCTTGTTCCAATTCTCAAATCTTCACTCTCTTTAACCCTCCAAGACTTTCTCCCTTTAGCTGGTAACATCGTTTGGCCTTTAGAATATCAACAACCAATAATCCAATATACTCCAAATGATGGAGTTTCCTTGATCATTGCAGAGTCTAATGCAGATTTCAATCATGTCACTGAAAATTCACCCCATGAAGCTTCTTTGTCTCGTTGTTTTGTACCACACTTAGAATCGACAGATTCTTTTGCTTCTATTATCTCAGTCCAGATAACCCTTTTTCCAGAAAGTGGTTTTTCCATTGGAATAAGCACTCATCATGCTGTTCTTGATGGAAAATCCTCAACCATGTTCATCAAAGCTTGGGCTTATCTATGCAACAAAACTATTGAAACAGAAGAACTACCAACTTTGTTGCCAGAGTTAAAACCTCTATTAGACAGGGAAATCATCAAAGACAACGGACTTGGTGAAAAGTTCACAAATAATTGGAGAAAGATCATAACCATGATGTTCCCAAATGAACAAGGAAACGAAAAATGCTTGAAGATTTTACCTTTTGAACCAAAACTCGAGGACTATGTTCGTGCTACATTCAAGCTCACACGTGAAGATTTGGATAAAATAAAGCAAATGGTGTTATCGAAATGGGAATTATTTGACACAAATGAATTGAATTCTAAGCCAAAATCTTTATCATCTTTTGTTCTCACTTGTGCCTATTCGCTTGTTTGTTTGGCAAAAGCAATTCATGGAGTTGAAAAGGAGAAAGAGAAGTTTGGGTTTGCATTCGCCGTAGATTGCAGAGCAAGGTTAGAACCACCATTACCAAATAATTATTTTGGAAATTGTGTATGGGGACATCTTGTTGATACAAAACCATTGGATTACATAAACGAAGATGGAGTGTTTTTAGTTGCTAAGTGTATTCATGAGAAAATAAACATGATAAATGAAAAGGGTGTTCTTGATGGAGCTAGTGATATGTTTGATAAATTCGCTTCTTTAGCAAGTGAAAAATTTGAAATGATGGGAGTTGCTGGGTCTAATAGATTTGGTGTTTATGAGATTGATTTTGGTTGGGGAAGACCAACAAAGGTGGAGATAGTTTCTGCGGATAGAGGTTTAACCATTGCTTTGGCGGAGAGCAAAGATAGTAAAGGTGGAATTGAAGTTGGGATTGTTAATAATAAGCATGTAATGAATCTCTTTAGAACTTTGTTTGTTGAAGGACTTTGCATTAATTGA

Protein sequence

>MS.gene44643.t1

MASNNNNNVKIHEHTKVFPPSSTRKTTTPLTYFDIFWLRFHPVERVFFYTLPNSHSHPSFFFKKLVPILKSSLSLTLQDFLPLAGNIVWPLEYQQPIIQYTPNDGVSLIIAESNADFNHVTENSPHEASLSRCFVPHLESTDSFASIISVQITLFPESGFSIGISTHHAVLDGKSSTMFIKAWAYLCNKTIETEELPTLLPELKPLLDREIIKDNGLGEKFTNNWRKIITMMFPNEQGNEKCLKILPFEPKLEDYVRATFKLTREDLDKIKQMVLSKWELFDTNELNSKPKSLSSFVLTCAYSLVCLAKAIHGVEKEKEKFGFAFAVDCRARLEPPLPNNYFGNCVWGHLVDTKPLDYINEDGVFLVAKCIHEKINMINEKGVLDGASDMFDKFASLASEKFEMMGVAGSNRFGVYEIDFGWGRPTKVEIVSADRGLTIALAESKDSKGGIEVGIVNNKHVMNLFRTLFVEGLCIN