AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene021915

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene021915.t1 MTR_7g014160 82.255 479 82 3 1 479 1 476 0.0 802
MS.gene021915.t1 MTR_7g014610 76.827 479 107 4 3 479 2 478 0.0 737
MS.gene021915.t1 MTR_7g014200 73.542 480 117 4 1 479 1 471 0.0 719
MS.gene021915.t1 MTR_7g014620 66.522 460 122 4 22 479 6 435 0.0 597
MS.gene021915.t1 MTR_7g014630 89.333 300 31 1 1 300 1 299 0.0 550
MS.gene021915.t1 MTR_7g013850 58.607 488 182 12 1 476 1 480 0.0 540
MS.gene021915.t1 MTR_7g014670 96.774 186 6 0 294 479 77 262 4.94e-129 375
MS.gene021915.t1 MTR_7g014230 41.962 479 251 12 7 478 2 460 2.09e-115 347
MS.gene021915.t1 MTR_7g014330 40.421 475 254 12 10 476 3 456 1.45e-108 330
MS.gene021915.t1 MTR_6g015815 41.087 460 248 10 19 476 22 460 1.00e-107 328
MS.gene021915.t1 MTR_7g014250 40.252 477 252 13 10 476 3 456 6.07e-105 320
MS.gene021915.t1 MTR_6g015830 39.232 469 258 12 11 476 16 460 7.65e-104 318
MS.gene021915.t1 MTR_6g015850 39.286 476 253 12 10 476 9 457 5.14e-103 315
MS.gene021915.t1 MTR_6g015320 41.042 480 242 18 7 476 6 454 5.82e-103 315
MS.gene021915.t1 MTR_6g015315 40.299 469 256 12 10 476 6 452 8.56e-102 312
MS.gene021915.t1 MTR_7g014180 77.907 172 36 2 6 177 4 173 1.86e-85 260
MS.gene021915.t1 MTR_3g028210 36.743 479 270 12 9 476 8 464 7.08e-85 269
MS.gene021915.t1 MTR_7g014360 35.452 409 210 11 79 476 21 386 1.04e-76 245
MS.gene021915.t1 MTR_2g089765 34.137 498 273 16 6 476 13 482 4.90e-75 244
MS.gene021915.t1 MTR_8g012750 32.335 501 279 14 1 474 3 470 4.15e-68 226
MS.gene021915.t1 MTR_3g087640 33.469 490 280 16 10 478 10 474 2.44e-67 224
MS.gene021915.t1 MTR_2g089670 32.043 465 273 12 27 476 41 477 4.55e-66 220
MS.gene021915.t1 MTR_7g015860 31.303 476 271 13 9 462 5 446 1.47e-63 213
MS.gene021915.t1 MTR_2g089735 33.038 451 258 14 44 477 39 462 2.30e-62 210
MS.gene021915.t1 MTR_2g089755 31.974 466 272 15 31 477 37 476 9.26e-58 198
MS.gene021915.t1 MTR_2g089650 30.909 495 291 17 1 476 1 463 3.66e-56 194
MS.gene021915.t1 MTR_6g015310 33.499 403 195 15 54 454 2 333 1.11e-54 186
MS.gene021915.t1 MTR_2g089665 28.698 453 192 17 9 450 2 334 3.92e-39 145
MS.gene021915.t1 MTR_7g014310 29.532 342 174 8 119 459 31 306 1.01e-38 143
MS.gene021915.t1 MTR_3g435300 30.182 275 163 8 20 282 13 270 1.84e-31 122
MS.gene021915.t1 MTR_4g127650 25.708 459 270 15 44 476 43 456 4.84e-24 104
MS.gene021915.t1 MTR_8g079950 25.696 467 285 16 12 469 8 421 3.08e-22 99.4
MS.gene021915.t1 MTR_7g086000 24.851 503 300 18 8 478 3 459 6.92e-21 95.5
MS.gene021915.t1 MTR_5g081960 23.554 484 315 16 20 465 6 472 7.00e-21 95.5
MS.gene021915.t1 MTR_3g070170 25.926 486 302 20 3 465 6 456 3.62e-20 93.6
MS.gene021915.t1 MTR_4g097090 24.893 466 293 17 30 476 29 456 1.27e-19 91.7
MS.gene021915.t1 MTR_3g026950 25.173 433 265 19 65 472 49 447 3.98e-19 90.1
MS.gene021915.t1 MTR_5g084870 25.319 470 283 20 12 472 8 418 1.11e-18 88.6
MS.gene021915.t1 MTR_3g049150 24.900 502 289 22 9 477 2 448 2.98e-17 84.3
MS.gene021915.t1 MTR_4g007540 24.040 495 282 25 9 474 3 432 3.71e-17 84.0
MS.gene021915.t1 MTR_7g014205 36.066 122 65 3 318 438 5 114 1.11e-16 76.6
MS.gene021915.t1 MTR_8g467580 25.339 442 249 20 8 428 4 385 1.46e-16 82.4
MS.gene021915.t1 MTR_3g102020 24.524 473 296 19 15 470 14 442 3.28e-16 81.3
MS.gene021915.t1 MTR_3g101960 24.070 457 276 18 31 465 29 436 4.48e-16 80.9
MS.gene021915.t1 MTR_4g022720 24.578 415 234 15 70 458 55 416 2.35e-15 78.6
MS.gene021915.t1 MTR_4g094828 24.078 461 273 20 27 461 25 434 2.91e-15 78.2
MS.gene021915.t1 MTR_7g014210 39.815 108 62 3 333 438 24 130 5.26e-15 73.2
MS.gene021915.t1 MTR_4g022670 24.845 483 273 19 18 467 14 439 1.95e-14 75.5
MS.gene021915.t1 MTR_3g026310 24.508 457 288 17 18 458 14 429 3.91e-14 74.7
MS.gene021915.t1 MTR_3g098980 23.602 483 304 18 1 473 6 433 3.94e-14 74.7
MS.gene021915.t1 MTR_4g022290 21.538 455 286 14 31 466 30 432 2.05e-13 72.4
MS.gene021915.t1 MTR_8g096530 21.730 474 309 15 5 469 3 423 2.79e-13 72.0
MS.gene021915.t1 MTR_8g079980 23.297 455 277 16 12 452 8 404 1.62e-12 69.7
MS.gene021915.t1 MTR_4g022800 23.861 461 280 15 18 458 14 423 1.65e-12 69.7
MS.gene021915.t1 MTR_7g014340 53.521 71 28 2 10 78 3 70 3.67e-12 63.2
MS.gene021915.t1 MTR_7g026860 25.060 415 235 15 70 458 55 419 3.68e-12 68.6
MS.gene021915.t1 MTR_7g014220 59.259 54 19 1 27 80 14 64 7.97e-12 61.6
MS.gene021915.t1 MTR_3g435290 34.188 117 73 3 360 476 5 117 1.53e-11 62.0
MS.gene021915.t1 MTR_7g027175 22.381 420 259 16 69 472 67 435 2.12e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene021915.t1 AT5G39050 40.084 479 263 15 6 477 7 468 1.38e-100 310
MS.gene021915.t1 AT5G39080 38.248 468 262 15 7 466 3 451 2.56e-98 303
MS.gene021915.t1 AT3G29590 37.526 477 259 11 7 477 6 449 3.44e-94 293
MS.gene021915.t1 AT3G29670 38.137 451 248 10 30 477 28 450 9.87e-93 289
MS.gene021915.t1 AT3G29635 36.000 450 266 9 30 478 28 456 1.73e-87 275
MS.gene021915.t1 AT5G39090 35.760 467 263 15 16 476 12 447 1.11e-85 271
MS.gene021915.t1 AT5G61160 31.049 467 296 8 9 472 3 446 4.58e-74 241
MS.gene021915.t1 AT3G29680 33.333 453 271 11 30 478 24 449 6.09e-73 238
MS.gene021915.t1 AT1G03940 31.633 490 278 14 10 476 9 464 9.40e-70 230
MS.gene021915.t1 AT1G03495 31.389 360 207 11 11 357 10 342 3.96e-44 159
MS.gene021915.t1 AT5G23940 26.096 479 295 24 6 466 2 439 2.13e-22 100
MS.gene021915.t1 AT5G41040 23.529 425 264 15 64 474 63 440 3.46e-21 96.3
MS.gene021915.t1 AT5G41040 23.529 425 264 15 64 474 79 456 4.73e-21 95.9
MS.gene021915.t1 AT2G19070 23.326 463 277 14 44 476 37 451 7.33e-19 89.4
MS.gene021915.t1 AT5G57840 23.422 491 304 21 9 479 3 441 2.98e-18 87.4
MS.gene021915.t1 AT2G39980 24.846 487 323 20 1 465 1 466 5.25e-18 87.0
MS.gene021915.t1 AT5G23970 24.783 460 265 17 16 460 9 402 8.21e-18 85.9
MS.gene021915.t1 AT3G29720 29.592 196 117 6 108 302 26 201 1.36e-16 79.3
MS.gene021915.t1 AT3G29690 29.487 156 95 4 326 476 33 178 2.71e-15 74.3
MS.gene021915.t1 AT5G07850 23.614 487 312 20 1 470 1 444 8.12e-14 73.9
MS.gene021915.t1 AT2G40230 22.785 474 308 18 7 471 3 427 2.80e-13 72.0
MS.gene021915.t1 AT3G48720 22.422 446 271 18 44 472 41 428 1.71e-11 66.6

Find 102 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACTTTCTTTGACATATTT+TGG 0.113161 7.1:-81695976 MS.gene021915:CDS
TCACTACCAAATAACTATTT+TGG 0.135502 7.1:-81695053 MS.gene021915:CDS
AATAACCACTTTCTGGAAAA+AGG 0.147614 7.1:+81695603 None:intergenic
GAAGGATGTGATTGTGAATT+TGG 0.152446 7.1:+81695911 None:intergenic
CCAATGGAATAACCACTTTC+TGG 0.167311 7.1:+81695596 None:intergenic
AAGAATCTGTTGATTCTAAA+TGG 0.182536 7.1:+81695654 None:intergenic
AGCAAATGGTGTTATCAAAA+TGG 0.193782 7.1:-81695247 MS.gene021915:CDS
GTGATAAGTTCACAAATAAT+TGG 0.223223 7.1:-81695406 MS.gene021915:CDS
GTTATCAAAATGGGAATTAT+TGG 0.234732 7.1:-81695237 MS.gene021915:CDS
GGTGTTTATGAGATTGATTT+TGG 0.265782 7.1:-81694819 MS.gene021915:CDS
CAATTTCCAAAATAGTTATT+TGG 0.276645 7.1:+81695047 None:intergenic
AGCGAGACAAAGAAGCTTTA+TGG 0.284458 7.1:+81695687 None:intergenic
AGCAATTCATGGAATTAAAA+TGG 0.285041 7.1:-81695132 MS.gene021915:CDS
AATTACAACCGTTGTTTGAT+TGG 0.287075 7.1:-81695457 MS.gene021915:CDS
GATAGTGAAGATTTGAGAAT+TGG 0.292165 7.1:+81695866 None:intergenic
TGCTTATGCCCTTGTTTGTT+TGG 0.306724 7.1:-81695159 MS.gene021915:CDS
CTTTATCCGGTAAAATTGTT+TGG 0.320853 7.1:-81695820 MS.gene021915:CDS
CGAAGAGAGTCTTCGAGCTT+TGG 0.323617 7.1:+81695311 None:intergenic
AAGATGTTCCCAAATGAAAA+AGG 0.331198 7.1:-81695368 MS.gene021915:CDS
CATCATCATTGGGATTGAAT+TGG 0.334138 7.1:+81695771 None:intergenic
GAGAAAGAGAAGTTTGCATT+TGG 0.340510 7.1:-81695110 MS.gene021915:CDS
TTTAAAACGTTGTTTCTTGA+AGG 0.351037 7.1:-81694666 MS.gene021915:CDS
AACTTATCACCAAGTTCATC+TGG 0.356756 7.1:+81695419 None:intergenic
GATGGAGTTTCCTTTATCAT+TGG 0.361065 7.1:-81695752 MS.gene021915:CDS
CAACAAAGGTTGTGGTTCTT+CGG 0.369447 7.1:+81695484 None:intergenic
CAACCATGTTTATCAAAGCT+TGG 0.376272 7.1:-81695535 MS.gene021915:CDS
ATCAGACTCTCCAATGATAA+AGG 0.377649 7.1:+81695742 None:intergenic
GAAACTATCTCCACCTTTGT+TGG 0.379376 7.1:+81694786 None:intergenic
AGAATCTGTTGATTCTAAAT+GGG 0.380644 7.1:+81695655 None:intergenic
GCGAGACAAAGAAGCTTTAT+GGG 0.390830 7.1:+81695688 None:intergenic
GTGGATAGAGGTTTAACAAT+TGG 0.394688 7.1:-81694762 MS.gene021915:CDS
AGTGAAGGATTTGAAATAAT+AGG 0.395317 7.1:-81694864 MS.gene021915:CDS
ACTCACCATGCTGTTCTTGA+TGG 0.395647 7.1:-81695566 MS.gene021915:CDS
AAAGGAAACTCCATCATCAT+TGG 0.396435 7.1:+81695760 None:intergenic
GAGTAACTTTAATGTGTTCA+TGG 0.397045 7.1:+81696035 None:intergenic
AAATTGTGTGTGGGGACATT+TGG 0.398292 7.1:-81695030 MS.gene021915:CDS
AACCATGTTTATCAAAGCTT+GGG 0.399105 7.1:-81695534 MS.gene021915:CDS
CAACACTTTCTTCCTTTATC+CGG 0.406614 7.1:-81695833 MS.gene021915:CDS
TACCATCTTTGCTCTCTGCC+AGG 0.406929 7.1:+81694739 None:intergenic
AAGGATGTGATTGTGAATTT+GGG 0.407904 7.1:+81695912 None:intergenic
TGTTTGGCAAAAGCAATTCA+TGG 0.416726 7.1:-81695143 MS.gene021915:CDS
GCAAATGGTGTTATCAAAAT+GGG 0.418223 7.1:-81695246 MS.gene021915:CDS
AATAAAGACGATAAATGAAA+AGG 0.420727 7.1:-81694937 MS.gene021915:CDS
AATATGTCAAAGAAAGTAAG+AGG 0.422390 7.1:+81695980 None:intergenic
GTGGCGGGATCTAATAGATT+TGG 0.423530 7.1:-81694840 MS.gene021915:CDS
CCAGAAAGTGGTTATTCCAT+TGG 0.423707 7.1:-81695596 MS.gene021915:CDS
GGTAAAGGTGGAATTGAAGT+TGG 0.427876 7.1:-81694720 MS.gene021915:CDS
GAGATAGTTTCGGTGGATAG+AGG 0.442745 7.1:-81694774 MS.gene021915:CDS
TTCAATTATTTGTCAAGTGA+AGG 0.447336 7.1:-81694879 MS.gene021915:CDS
ATAAAGACGATAAATGAAAA+GGG 0.449987 7.1:-81694936 MS.gene021915:CDS
AATGAAAAGGGTGTTCTTGA+AGG 0.453937 7.1:-81694924 MS.gene021915:CDS
TTGGATTACATAAATGAAGA+TGG 0.466086 7.1:-81694993 MS.gene021915:CDS
GTAAAGGTGGAATTGAAGTT+GGG 0.475472 7.1:-81694719 MS.gene021915:CDS
CAATTCAATCCCAATGATGA+TGG 0.476347 7.1:-81695770 MS.gene021915:CDS
TAGAAGAAAATACGTTCTAC+AGG 0.480691 7.1:+81695941 None:intergenic
GAGAACAAACGATGATAAAG+TGG 0.484849 7.1:+81695187 None:intergenic
TTTGAACAAGATAAAGCAAA+TGG 0.487133 7.1:-81695261 MS.gene021915:CDS
TTGGGATTGAATTGGATGAA+TGG 0.488013 7.1:+81695779 None:intergenic
AAGGAAACTCCATCATCATT+GGG 0.490933 7.1:+81695761 None:intergenic
GTTCTTGAAGGAGCTAGTGA+TGG 0.493087 7.1:-81694912 MS.gene021915:CDS
TTCTTGAAGGAGCTAGTGAT+GGG 0.497785 7.1:-81694911 MS.gene021915:CDS
AACAAAGGTGGAGATAGTTT+CGG 0.498546 7.1:-81694784 MS.gene021915:CDS
AATTCTTGCAACAAAGGTTG+TGG 0.499343 7.1:+81695476 None:intergenic
TTTAATGTTGCTCTTGTCGA+TGG 0.499466 7.1:+81696060 None:intergenic
GGTTGTAATTCTTGCAACAA+AGG 0.507240 7.1:+81695470 None:intergenic
TGGATTACATAAATGAAGAT+GGG 0.512542 7.1:-81694992 MS.gene021915:CDS
TAGAGGTTTAACAATTGGCC+TGG 0.518320 7.1:-81694757 MS.gene021915:CDS
TTTGGTTGACACAAAACCGT+TGG 0.520534 7.1:-81695012 MS.gene021915:CDS
AAATAGTTATTTGGTAGTGA+TGG 0.521146 7.1:+81695056 None:intergenic
GTCAAAGACCCAGATGAACT+TGG 0.526008 7.1:-81695428 MS.gene021915:CDS
TTGAATTGGATGAATGGTTG+TGG 0.526009 7.1:+81695785 None:intergenic
TGTTCTAGATAAGCATGTAA+TGG 0.534624 7.1:-81694694 MS.gene021915:CDS
GGCCTGGCAGAGAGCAAAGA+TGG 0.534800 7.1:-81694741 MS.gene021915:CDS
CGGATAAAGGAAGAAAGTGT+TGG 0.538758 7.1:+81695834 None:intergenic
TAACCTTGCTCTGCAATCTA+CGG 0.549902 7.1:+81695082 None:intergenic
AGAAAATACGTTCTACAGGA+TGG 0.553328 7.1:+81695945 None:intergenic
TCTTCATTTATGTAATCCAA+CGG 0.557148 7.1:+81694996 None:intergenic
TTTGAAATAATAGGAGTGGC+GGG 0.565832 7.1:-81694855 MS.gene021915:CDS
AGCCCAAGCTTTGATAAACA+TGG 0.576503 7.1:+81695532 None:intergenic
ATTTGAAATAATAGGAGTGG+CGG 0.578943 7.1:-81694856 MS.gene021915:CDS
GCAGAGAGCAAAGATGGTAA+AGG 0.579752 7.1:-81694735 MS.gene021915:CDS
GAATCTGTTGATTCTAAATG+GGG 0.580465 7.1:+81695656 None:intergenic
TAAAGGAAGAAAGTGTTGGA+GGG 0.602546 7.1:+81695838 None:intergenic
ACTTATCACCAAGTTCATCT+GGG 0.602703 7.1:+81695420 None:intergenic
ATGGTGAGTGCTTATTCCAA+TGG 0.605931 7.1:+81695580 None:intergenic
ATAAAGGAAGAAAGTGTTGG+AGG 0.607267 7.1:+81695837 None:intergenic
TCACCGTAGATTGCAGAGCA+AGG 0.607885 7.1:-81695085 MS.gene021915:CDS
ATATGTCAAAGAAAGTAAGA+GGG 0.609956 7.1:+81695981 None:intergenic
GTGGGGAAGACCAACAAAGG+TGG 0.612550 7.1:-81694796 MS.gene021915:CDS
GAGAGCAAAGATGGTAAAGG+TGG 0.619484 7.1:-81694732 MS.gene021915:CDS
AGGATTTGAAATAATAGGAG+TGG 0.624166 7.1:-81694859 MS.gene021915:CDS
TGGGTGGGGAAGACCAACAA+AGG 0.627130 7.1:-81694799 MS.gene021915:CDS
AAAGGTGGAGATAGTTTCGG+TGG 0.628419 7.1:-81694781 MS.gene021915:CDS
TATGTCAAAGAAAGTAAGAG+GGG 0.629256 7.1:+81695982 None:intergenic
ACGATATTCCAATCAAACAA+CGG 0.632005 7.1:+81695449 None:intergenic
GGATTACATAAATGAAGATG+GGG 0.640685 7.1:-81694991 MS.gene021915:CDS
GATGTGATTGTGAATTTGGG+AGG 0.652869 7.1:+81695915 None:intergenic
AATGGTTGTGGAGAATCAGA+AGG 0.653744 7.1:+81695797 None:intergenic
ATGTGATTGTGAATTTGGGA+GGG 0.663766 7.1:+81695916 None:intergenic
CGAGACAAAGAAGCTTTATG+GGG 0.673549 7.1:+81695689 None:intergenic
ACAAACGATGATAAAGTGGG+TGG 0.683948 7.1:+81695191 None:intergenic
AGAACAAACGATGATAAAGT+GGG 0.738379 7.1:+81695188 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAGACGATAAATGAAA+AGG - chr7.1:81695780-81695799 MS.gene021915:CDS 20.0%
!! ATAAAGACGATAAATGAAAA+GGG - chr7.1:81695781-81695800 MS.gene021915:CDS 20.0%
!! CAATTTCCAAAATAGTTATT+TGG + chr7.1:81695673-81695692 None:intergenic 20.0%
!!! TTACTTTCTTTGACATATTT+TGG - chr7.1:81694741-81694760 MS.gene021915:CDS 20.0%
! AATATGTCAAAGAAAGTAAG+AGG + chr7.1:81694740-81694759 None:intergenic 25.0%
! AGCAATTCATGGAATTAAAA+TGG - chr7.1:81695585-81695604 MS.gene021915:CDS 25.0%
! ATATGTCAAAGAAAGTAAGA+GGG + chr7.1:81694739-81694758 None:intergenic 25.0%
! GTGATAAGTTCACAAATAAT+TGG - chr7.1:81695311-81695330 MS.gene021915:CDS 25.0%
! GTTATCAAAATGGGAATTAT+TGG - chr7.1:81695480-81695499 MS.gene021915:CDS 25.0%
! TCTTCATTTATGTAATCCAA+CGG + chr7.1:81695724-81695743 None:intergenic 25.0%
! TGGATTACATAAATGAAGAT+GGG - chr7.1:81695725-81695744 MS.gene021915:CDS 25.0%
! TTCAATTATTTGTCAAGTGA+AGG - chr7.1:81695838-81695857 MS.gene021915:CDS 25.0%
! TTGGATTACATAAATGAAGA+TGG - chr7.1:81695724-81695743 MS.gene021915:CDS 25.0%
! TTTGAACAAGATAAAGCAAA+TGG - chr7.1:81695456-81695475 MS.gene021915:CDS 25.0%
!! AGTGAAGGATTTGAAATAAT+AGG - chr7.1:81695853-81695872 MS.gene021915:CDS 25.0%
!! TCACTACCAAATAACTATTT+TGG - chr7.1:81695664-81695683 MS.gene021915:CDS 25.0%
!!! AAATAGTTATTTGGTAGTGA+TGG + chr7.1:81695664-81695683 None:intergenic 25.0%
!!! AAGAATCTGTTGATTCTAAA+TGG + chr7.1:81695066-81695085 None:intergenic 25.0%
!!! AGAATCTGTTGATTCTAAAT+GGG + chr7.1:81695065-81695084 None:intergenic 25.0%
!!! AGTTTTTGGAAAAAGAAAGA+AGG + chr7.1:81694827-81694846 None:intergenic 25.0%
!!! GTGTTTATGAGATTGATTTT+GGG - chr7.1:81695899-81695918 MS.gene021915:CDS 25.0%
!!! TTTAAAACGTTGTTTCTTGA+AGG - chr7.1:81696051-81696070 MS.gene021915:CDS 25.0%
!!! TTTCGTTTCCTTTTTCATTT+GGG + chr7.1:81695360-81695379 None:intergenic 25.0%
AACCATGTTTATCAAAGCTT+GGG - chr7.1:81695183-81695202 MS.gene021915:CDS 30.0%
AAGATGTTCCCAAATGAAAA+AGG - chr7.1:81695349-81695368 MS.gene021915:CDS 30.0%
AAGGATGTGATTGTGAATTT+GGG + chr7.1:81694808-81694827 None:intergenic 30.0%
AATTACAACCGTTGTTTGAT+TGG - chr7.1:81695260-81695279 MS.gene021915:CDS 30.0%
ACGATATTCCAATCAAACAA+CGG + chr7.1:81695271-81695290 None:intergenic 30.0%
AGAACAAACGATGATAAAGT+GGG + chr7.1:81695532-81695551 None:intergenic 30.0%
CTTTATCCGGTAAAATTGTT+TGG - chr7.1:81694897-81694916 MS.gene021915:CDS 30.0%
GAGTAACTTTAATGTGTTCA+TGG + chr7.1:81694685-81694704 None:intergenic 30.0%
GATAGTGAAGATTTGAGAAT+TGG + chr7.1:81694854-81694873 None:intergenic 30.0%
GGATTACATAAATGAAGATG+GGG - chr7.1:81695726-81695745 MS.gene021915:CDS 30.0%
TAGAAGAAAATACGTTCTAC+AGG + chr7.1:81694779-81694798 None:intergenic 30.0%
TATGTCAAAGAAAGTAAGAG+GGG + chr7.1:81694738-81694757 None:intergenic 30.0%
! AATAACCACTTTCTGGAAAA+AGG + chr7.1:81695117-81695136 None:intergenic 30.0%
! AGGATTTGAAATAATAGGAG+TGG - chr7.1:81695858-81695877 MS.gene021915:CDS 30.0%
! ATAACCACTTTCTGGAAAAA+GGG + chr7.1:81695116-81695135 None:intergenic 30.0%
! ATTTGAAATAATAGGAGTGG+CGG - chr7.1:81695861-81695880 MS.gene021915:CDS 30.0%
! CAAACAATTTTACCGGATAA+AGG + chr7.1:81694899-81694918 None:intergenic 30.0%
! TGAGAATTGGAACAAGTTTT+TGG + chr7.1:81694841-81694860 None:intergenic 30.0%
! TGTTCTAGATAAGCATGTAA+TGG - chr7.1:81696023-81696042 MS.gene021915:CDS 30.0%
!! ACTATTTTGGAAATTGTGTG+TGG - chr7.1:81695677-81695696 MS.gene021915:CDS 30.0%
!! AGCAAATGGTGTTATCAAAA+TGG - chr7.1:81695470-81695489 MS.gene021915:CDS 30.0%
!! CTATTTTGGAAATTGTGTGT+GGG - chr7.1:81695678-81695697 MS.gene021915:CDS 30.0%
!! CTTTCGTTTCCTTTTTCATT+TGG + chr7.1:81695361-81695380 None:intergenic 30.0%
!! GAATCTGTTGATTCTAAATG+GGG + chr7.1:81695064-81695083 None:intergenic 30.0%
!! GCAAATGGTGTTATCAAAAT+GGG - chr7.1:81695471-81695490 MS.gene021915:CDS 30.0%
!! GGTGTTTATGAGATTGATTT+TGG - chr7.1:81695898-81695917 MS.gene021915:CDS 30.0%
!! TATTTTGGAAATTGTGTGTG+GGG - chr7.1:81695679-81695698 MS.gene021915:CDS 30.0%
!! TTATGAGATTGATTTTGGGT+GGG - chr7.1:81695903-81695922 MS.gene021915:CDS 30.0%
!! TTTATGAGATTGATTTTGGG+TGG - chr7.1:81695902-81695921 MS.gene021915:CDS 30.0%
!!! TCTTTGACATATTTTGGCTT+AGG - chr7.1:81694747-81694766 MS.gene021915:CDS 30.0%
AAAGGAAACTCCATCATCAT+TGG + chr7.1:81694960-81694979 None:intergenic 35.0%
AACAAAGGTGGAGATAGTTT+CGG - chr7.1:81695933-81695952 MS.gene021915:CDS 35.0%
AACTTATCACCAAGTTCATC+TGG + chr7.1:81695301-81695320 None:intergenic 35.0%
AAGGAAACTCCATCATCATT+GGG + chr7.1:81694959-81694978 None:intergenic 35.0%
AATTCTTGCAACAAAGGTTG+TGG + chr7.1:81695244-81695263 None:intergenic 35.0%
ACTTATCACCAAGTTCATCT+GGG + chr7.1:81695300-81695319 None:intergenic 35.0%
AGAAAATACGTTCTACAGGA+TGG + chr7.1:81694775-81694794 None:intergenic 35.0%
ATCAGACTCTCCAATGATAA+AGG + chr7.1:81694978-81694997 None:intergenic 35.0%
ATGTGATTGTGAATTTGGGA+GGG + chr7.1:81694804-81694823 None:intergenic 35.0%
CAACACTTTCTTCCTTTATC+CGG - chr7.1:81694884-81694903 MS.gene021915:CDS 35.0%
CAACCATGTTTATCAAAGCT+TGG - chr7.1:81695182-81695201 MS.gene021915:CDS 35.0%
CAATTCAATCCCAATGATGA+TGG - chr7.1:81694947-81694966 MS.gene021915:CDS 35.0%
CATCATCATTGGGATTGAAT+TGG + chr7.1:81694949-81694968 None:intergenic 35.0%
GAAGGATGTGATTGTGAATT+TGG + chr7.1:81694809-81694828 None:intergenic 35.0%
GAGAAAGAGAAGTTTGCATT+TGG - chr7.1:81695607-81695626 MS.gene021915:CDS 35.0%
GAGAACAAACGATGATAAAG+TGG + chr7.1:81695533-81695552 None:intergenic 35.0%
GATGGAGTTTCCTTTATCAT+TGG - chr7.1:81694965-81694984 MS.gene021915:CDS 35.0%
GGTTGTAATTCTTGCAACAA+AGG + chr7.1:81695250-81695269 None:intergenic 35.0%
GTAAAGGTGGAATTGAAGTT+GGG - chr7.1:81695998-81696017 MS.gene021915:CDS 35.0%
GTGGATAGAGGTTTAACAAT+TGG - chr7.1:81695955-81695974 MS.gene021915:CDS 35.0%
TGTTTGGCAAAAGCAATTCA+TGG - chr7.1:81695574-81695593 MS.gene021915:CDS 35.0%
TTGAATTGGATGAATGGTTG+TGG + chr7.1:81694935-81694954 None:intergenic 35.0%
TTGGGATTGAATTGGATGAA+TGG + chr7.1:81694941-81694960 None:intergenic 35.0%
TTTAATGTTGCTCTTGTCGA+TGG + chr7.1:81694660-81694679 None:intergenic 35.0%
! AGAAGGCCAAACAATTTTAC+CGG + chr7.1:81694906-81694925 None:intergenic 35.0%
! ATAACCCTTTTTCCAGAAAG+TGG - chr7.1:81695109-81695128 MS.gene021915:CDS 35.0%
! ATTTTCCATCAAGAACAGCA+TGG + chr7.1:81695159-81695178 None:intergenic 35.0%
! TGCTTTTGCCAAACAAACAA+GGG + chr7.1:81695569-81695588 None:intergenic 35.0%
! TTTCTGGAAAAAGGGTTATC+TGG + chr7.1:81695108-81695127 None:intergenic 35.0%
! TTTGAAATAATAGGAGTGGC+GGG - chr7.1:81695862-81695881 MS.gene021915:CDS 35.0%
!! AATGAAAAGGGTGTTCTTGA+AGG - chr7.1:81695793-81695812 MS.gene021915:CDS 35.0%
!! ATAAAGGAAGAAAGTGTTGG+AGG + chr7.1:81694883-81694902 None:intergenic 35.0%
!! TAAAGGAAGAAAGTGTTGGA+GGG + chr7.1:81694882-81694901 None:intergenic 35.0%
!! TATGAGATTGATTTTGGGTG+GGG - chr7.1:81695904-81695923 MS.gene021915:CDS 35.0%
!! TTGCTTTTGCCAAACAAACA+AGG + chr7.1:81695570-81695589 None:intergenic 35.0%
!!! AGGGGTTGTTGTTGTTTTTT+GGG + chr7.1:81694720-81694739 None:intergenic 35.0%
!!! GTTGTTTTTTGGGTTGATGA+TGG + chr7.1:81694710-81694729 None:intergenic 35.0%
AAATTGTGTGTGGGGACATT+TGG - chr7.1:81695687-81695706 MS.gene021915:CDS 40.0%
AATGGTTGTGGAGAATCAGA+AGG + chr7.1:81694923-81694942 None:intergenic 40.0%
ACAAACGATGATAAAGTGGG+TGG + chr7.1:81695529-81695548 None:intergenic 40.0%
AGCGAGACAAAGAAGCTTTA+TGG + chr7.1:81695033-81695052 None:intergenic 40.0%
ATGGTGAGTGCTTATTCCAA+TGG + chr7.1:81695140-81695159 None:intergenic 40.0%
CAACAAAGGTTGTGGTTCTT+CGG + chr7.1:81695236-81695255 None:intergenic 40.0%
CCAATGGAATAACCACTTTC+TGG + chr7.1:81695124-81695143 None:intergenic 40.0%
CCAGAAAGTGGTTATTCCAT+TGG - chr7.1:81695121-81695140 MS.gene021915:CDS 40.0%
GAAACTATCTCCACCTTTGT+TGG + chr7.1:81695934-81695953 None:intergenic 40.0%
GATGTGATTGTGAATTTGGG+AGG + chr7.1:81694805-81694824 None:intergenic 40.0%
GGTAAAGGTGGAATTGAAGT+TGG - chr7.1:81695997-81696016 MS.gene021915:CDS 40.0%
TAACCTTGCTCTGCAATCTA+CGG + chr7.1:81695638-81695657 None:intergenic 40.0%
TAGAGGTTTAACAATTGGCC+TGG - chr7.1:81695960-81695979 MS.gene021915:CDS 40.0%
TTCTTGAAGGAGCTAGTGAT+GGG - chr7.1:81695806-81695825 MS.gene021915:CDS 40.0%
TTTGGTTGACACAAAACCGT+TGG - chr7.1:81695705-81695724 MS.gene021915:CDS 40.0%
! AGCCCAAGCTTTGATAAACA+TGG + chr7.1:81695188-81695207 None:intergenic 40.0%
! CGAGACAAAGAAGCTTTATG+GGG + chr7.1:81695031-81695050 None:intergenic 40.0%
! CGGATAAAGGAAGAAAGTGT+TGG + chr7.1:81694886-81694905 None:intergenic 40.0%
! GCGAGACAAAGAAGCTTTAT+GGG + chr7.1:81695032-81695051 None:intergenic 40.0%
! TGCTTATGCCCTTGTTTGTT+TGG - chr7.1:81695558-81695577 MS.gene021915:CDS 40.0%
!!! GAGGGGTTGTTGTTGTTTTT+TGG + chr7.1:81694721-81694740 None:intergenic 40.0%
!!! GTTTTTTGGGTTGATGATGG+AGG + chr7.1:81694707-81694726 None:intergenic 40.0%
AAAGGTGGAGATAGTTTCGG+TGG - chr7.1:81695936-81695955 MS.gene021915:CDS 45.0%
ACTCACCATGCTGTTCTTGA+TGG - chr7.1:81695151-81695170 MS.gene021915:CDS 45.0%
GAGAGCAAAGATGGTAAAGG+TGG - chr7.1:81695985-81696004 MS.gene021915:CDS 45.0%
GAGATAGTTTCGGTGGATAG+AGG - chr7.1:81695943-81695962 MS.gene021915:CDS 45.0%
GCAGAGAGCAAAGATGGTAA+AGG - chr7.1:81695982-81696001 MS.gene021915:CDS 45.0%
GTCAAAGACCCAGATGAACT+TGG - chr7.1:81695289-81695308 MS.gene021915:CDS 45.0%
GTTCTTGAAGGAGCTAGTGA+TGG - chr7.1:81695805-81695824 MS.gene021915:CDS 45.0%
! GTGGCGGGATCTAATAGATT+TGG - chr7.1:81695877-81695896 MS.gene021915:CDS 45.0%
CGAAGAGAGTCTTCGAGCTT+TGG + chr7.1:81695409-81695428 None:intergenic 50.0%
TACCATCTTTGCTCTCTGCC+AGG + chr7.1:81695981-81696000 None:intergenic 50.0%
TCACCGTAGATTGCAGAGCA+AGG - chr7.1:81695632-81695651 MS.gene021915:CDS 50.0%
GTGGGGAAGACCAACAAAGG+TGG - chr7.1:81695921-81695940 MS.gene021915:CDS 55.0%
TGGGTGGGGAAGACCAACAA+AGG - chr7.1:81695918-81695937 MS.gene021915:CDS 55.0%
GGCCTGGCAGAGAGCAAAGA+TGG - chr7.1:81695976-81695995 MS.gene021915:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 81694650 81696089 81694650 ID=MS.gene021915
chr7.1 mRNA 81694650 81696089 81694650 ID=MS.gene021915.t1;Parent=MS.gene021915
chr7.1 exon 81694650 81696089 81694650 ID=MS.gene021915.t1.exon1;Parent=MS.gene021915.t1
chr7.1 CDS 81694650 81696089 81694650 ID=cds.MS.gene021915.t1;Parent=MS.gene021915.t1
Gene Sequence

>MS.gene021915

ATGGATTCCATCGACAAGAGCAACATTAAAATCCATGAACACATTAAAGTTACTCCTCCATCATCAACCCAAAAAACAACAACAACCCCTCTTACTTTCTTTGACATATTTTGGCTTAGGTTCCATCCTGTAGAACGTATTTTCTTCTACACCCTCCCAAATTCACAATCACATCCTTCTTTCTTTTTCCAAAAACTTGTTCCAATTCTCAAATCTTCACTATCTTTAACCCTCCAACACTTTCTTCCTTTATCCGGTAAAATTGTTTGGCCTTCTGATTCTCCACAACCATTCATCCAATTCAATCCCAATGATGATGGAGTTTCCTTTATCATTGGAGAGTCTGATTTAGATTTCAATCATGTTATTGAAAACTCACCCCATAAAGCTTCTTTGTCTCGCTCTTTTATTCCCCATTTAGAATCAACAGATTCTTTTGCTTCTATTATCTCAGTCCAGATAACCCTTTTTCCAGAAAGTGGTTATTCCATTGGAATAAGCACTCACCATGCTGTTCTTGATGGAAAATCTTCAACCATGTTTATCAAAGCTTGGGCTTATCTATGCAACAAAAACATTGAAACCGAAGAACCACAACCTTTGTTGCAAGAATTACAACCGTTGTTTGATTGGAATATCGTCAAAGACCCAGATGAACTTGGTGATAAGTTCACAAATAATTGGAGAGAGATCGTTACTAAGATGTTCCCAAATGAAAAAGGAAACGAAAGATGCTTGAAGATTTTAGCTTTTGAACCAAAGCTCGAAGACTCTCTTCGTGCTACGTTCAAGCTCACACGTGAAGATTTGAACAAGATAAAGCAAATGGTGTTATCAAAATGGGAATTATTGGACACAAATGAATTAAATTCTAAGCCACCCACTTTATCATCGTTTGTTCTCACATGTGCTTATGCCCTTGTTTGTTTGGCAAAAGCAATTCATGGAATTAAAATGGAGAAAGAGAAGTTTGCATTTGGATTCACCGTAGATTGCAGAGCAAGGTTAGAGCCATCACTACCAAATAACTATTTTGGAAATTGTGTGTGGGGACATTTGGTTGACACAAAACCGTTGGATTACATAAATGAAGATGGGGTGTTTTTAGTTGCTAAGTGTATTCATGAGAAAATAAAGACGATAAATGAAAAGGGTGTTCTTGAAGGAGCTAGTGATGGGTTTAGTAAATTCAATTATTTGTCAAGTGAAGGATTTGAAATAATAGGAGTGGCGGGATCTAATAGATTTGGTGTTTATGAGATTGATTTTGGGTGGGGAAGACCAACAAAGGTGGAGATAGTTTCGGTGGATAGAGGTTTAACAATTGGCCTGGCAGAGAGCAAAGATGGTAAAGGTGGAATTGAAGTTGGGCTTGTTCTAGATAAGCATGTAATGGATCTCTTTAAAACGTTGTTTCTTGAAGGACTTCACATTAATTGA
Protein sequence

>MS.gene021915.t1

MDSIDKSNIKIHEHIKVTPPSSTQKTTTTPLTFFDIFWLRFHPVERIFFYTLPNSQSHPSFFFQKLVPILKSSLSLTLQHFLPLSGKIVWPSDSPQPFIQFNPNDDGVSFIIGESDLDFNHVIENSPHKASLSRSFIPHLESTDSFASIISVQITLFPESGYSIGISTHHAVLDGKSSTMFIKAWAYLCNKNIETEEPQPLLQELQPLFDWNIVKDPDELGDKFTNNWREIVTKMFPNEKGNERCLKILAFEPKLEDSLRATFKLTREDLNKIKQMVLSKWELLDTNELNSKPPTLSSFVLTCAYALVCLAKAIHGIKMEKEKFAFGFTVDCRARLEPSLPNNYFGNCVWGHLVDTKPLDYINEDGVFLVAKCIHEKIKTINEKGVLEGASDGFSKFNYLSSEGFEIIGVAGSNRFGVYEIDFGWGRPTKVEIVSVDRGLTIGLAESKDGKGGIEVGLVLDKHVMDLFKTLFLEGLHIN