AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene91598

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91598.t1 MTR_7g014610 82.143 476 85 0 1 476 1 476 0.0 796
MS.gene91598.t1 MTR_7g014160 74.262 474 119 3 4 477 5 475 0.0 726
MS.gene91598.t1 MTR_7g014620 81.878 458 54 1 20 477 6 434 0.0 723
MS.gene91598.t1 MTR_7g014200 71.548 478 125 5 1 476 1 469 0.0 697
MS.gene91598.t1 MTR_7g013850 57.787 488 189 10 1 477 1 482 0.0 530
MS.gene91598.t1 MTR_7g014630 71.141 298 84 2 2 298 3 299 6.39e-148 427
MS.gene91598.t1 MTR_7g014230 41.788 481 245 13 9 478 5 461 1.74e-112 340
MS.gene91598.t1 MTR_7g014330 40.919 479 248 12 9 476 3 457 4.38e-111 337
MS.gene91598.t1 MTR_6g015320 40.842 475 246 13 8 475 8 454 6.39e-107 326
MS.gene91598.t1 MTR_6g015815 40.619 485 253 14 1 475 1 460 4.31e-106 324
MS.gene91598.t1 MTR_6g015850 40.246 487 249 14 1 475 1 457 9.84e-106 323
MS.gene91598.t1 MTR_7g014250 41.139 474 252 13 9 475 3 456 2.01e-105 322
MS.gene91598.t1 MTR_6g015830 40.123 486 254 16 1 475 1 460 2.17e-102 314
MS.gene91598.t1 MTR_6g015315 39.362 470 259 11 9 475 6 452 3.04e-99 306
MS.gene91598.t1 MTR_7g014670 71.354 192 52 2 284 475 71 259 1.57e-95 290
MS.gene91598.t1 MTR_7g014180 72.515 171 47 0 4 174 3 173 1.28e-83 256
MS.gene91598.t1 MTR_2g089765 34.073 496 270 14 8 475 16 482 3.08e-76 247
MS.gene91598.t1 MTR_3g028210 33.817 482 280 12 8 475 8 464 9.03e-76 246
MS.gene91598.t1 MTR_7g014360 35.627 407 213 9 78 476 22 387 2.57e-75 242
MS.gene91598.t1 MTR_8g012750 32.400 500 283 17 8 477 3 477 3.92e-65 218
MS.gene91598.t1 MTR_2g089670 32.468 462 276 12 25 475 41 477 2.64e-64 216
MS.gene91598.t1 MTR_7g015860 32.784 485 272 11 5 468 2 453 3.07e-63 213
MS.gene91598.t1 MTR_3g087640 32.515 489 282 16 9 475 10 472 2.84e-60 205
MS.gene91598.t1 MTR_2g089735 32.962 449 260 12 42 475 39 461 3.96e-59 202
MS.gene91598.t1 MTR_2g089650 33.264 484 273 15 16 480 16 468 6.78e-59 201
MS.gene91598.t1 MTR_2g089755 33.691 466 263 14 29 475 37 475 1.38e-57 198
MS.gene91598.t1 MTR_6g015310 32.673 404 189 13 56 453 7 333 5.75e-54 184
MS.gene91598.t1 MTR_7g014310 30.347 346 172 8 116 460 31 308 3.76e-37 139
MS.gene91598.t1 MTR_2g089665 27.214 463 202 14 8 458 2 341 8.59e-37 139
MS.gene91598.t1 MTR_3g435300 30.208 288 164 9 9 280 4 270 2.74e-29 116
MS.gene91598.t1 MTR_5g081960 23.909 481 316 18 14 464 12 472 2.11e-23 103
MS.gene91598.t1 MTR_7g086000 24.597 496 312 17 7 478 3 460 1.78e-22 100
MS.gene91598.t1 MTR_8g075610 24.685 397 233 16 45 427 30 374 3.20e-21 96.3
MS.gene91598.t1 MTR_8g096530 22.961 466 311 12 4 467 3 422 4.72e-21 95.9
MS.gene91598.t1 MTR_3g026950 23.299 485 298 20 16 472 10 448 1.08e-20 94.7
MS.gene91598.t1 MTR_4g127650 23.591 479 311 16 15 476 17 457 3.14e-20 93.6
MS.gene91598.t1 MTR_3g070170 24.897 486 303 18 4 464 8 456 1.40e-19 91.7
MS.gene91598.t1 MTR_4g097090 22.917 480 310 16 16 475 17 456 1.16e-18 89.0
MS.gene91598.t1 MTR_3g098980 24.257 404 263 13 67 467 65 428 4.42e-18 86.7
MS.gene91598.t1 MTR_4g022825 23.391 466 280 15 15 457 10 421 1.01e-17 85.9
MS.gene91598.t1 MTR_4g007540 23.043 447 273 20 45 473 39 432 1.67e-17 85.1
MS.gene91598.t1 MTR_4g022800 23.800 479 291 18 8 465 4 429 7.24e-17 83.2
MS.gene91598.t1 MTR_3g101960 23.975 488 294 21 18 480 18 453 1.33e-16 82.4
MS.gene91598.t1 MTR_4g094828 21.535 469 284 14 25 465 25 437 1.49e-16 82.4
MS.gene91598.t1 MTR_8g467580 23.272 434 244 16 19 427 16 385 1.84e-16 82.0
MS.gene91598.t1 MTR_4g094835 23.467 473 273 20 20 465 23 433 2.83e-16 81.3
MS.gene91598.t1 MTR_7g014210 38.889 108 64 2 331 437 24 130 4.76e-16 76.3
MS.gene91598.t1 MTR_7g014205 35.200 125 59 4 318 437 7 114 1.00e-15 73.9
MS.gene91598.t1 MTR_3g049150 23.884 448 254 20 64 475 51 447 1.90e-15 79.0
MS.gene91598.t1 MTR_7g027175 22.532 466 308 17 7 467 14 431 2.08e-15 78.6
MS.gene91598.t1 MTR_3g102020 24.771 436 256 19 18 431 18 403 2.58e-15 78.6
MS.gene91598.t1 MTR_8g079950 23.543 446 272 15 19 451 16 405 2.81e-15 78.2
MS.gene91598.t1 MTR_8g079710 22.500 480 267 18 27 470 27 437 4.74e-15 77.4
MS.gene91598.t1 MTR_4g022290 23.144 458 260 15 27 455 28 422 7.76e-15 77.0
MS.gene91598.t1 MTR_7g085930 22.581 434 289 11 68 480 59 466 1.57e-14 76.3
MS.gene91598.t1 MTR_3g026310 25.821 457 265 19 8 439 4 411 2.22e-14 75.5
MS.gene91598.t1 MTR_4g022670 26.196 439 242 19 18 431 15 396 2.98e-14 75.1
MS.gene91598.t1 MTR_7g084910 23.202 431 264 14 3 428 12 380 7.35e-14 73.9
MS.gene91598.t1 MTR_7g014340 46.739 92 39 3 9 97 3 87 7.51e-14 67.8
MS.gene91598.t1 MTR_7g084940 24.217 479 302 17 8 475 16 444 8.35e-14 73.6
MS.gene91598.t1 MTR_0190s0020 24.821 419 233 19 68 466 63 419 8.51e-14 73.6
MS.gene91598.t1 MTR_2g007880 23.598 428 239 16 67 467 73 439 9.60e-14 73.6
MS.gene91598.t1 MTR_8g041770 24.889 450 271 19 26 466 27 418 4.82e-13 71.2
MS.gene91598.t1 MTR_8g467590 22.538 457 265 16 7 440 4 394 5.05e-13 71.2
MS.gene91598.t1 MTR_7g014220 50.000 68 26 2 25 92 14 73 1.03e-12 63.9
MS.gene91598.t1 MTR_7g026860 24.138 435 253 16 15 427 10 389 2.31e-12 69.3
MS.gene91598.t1 MTR_3g435290 45.882 85 41 2 394 475 35 117 8.20e-12 62.8
MS.gene91598.t1 MTR_7g114520 23.529 306 190 9 45 346 48 313 2.16e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91598.t1 AT5G39050 40.167 478 260 15 6 475 8 467 7.80e-97 300
MS.gene91598.t1 AT5G39080 39.659 469 254 14 6 465 3 451 1.97e-95 296
MS.gene91598.t1 AT3G29590 36.402 478 263 13 5 475 5 448 2.85e-87 275
MS.gene91598.t1 AT3G29670 37.916 451 250 10 27 475 27 449 1.95e-86 273
MS.gene91598.t1 AT5G39090 37.234 470 257 14 13 475 9 447 3.94e-85 269
MS.gene91598.t1 AT3G29635 36.463 458 260 11 26 478 26 457 4.79e-85 270
MS.gene91598.t1 AT5G61160 33.617 470 284 10 8 473 3 448 8.05e-79 253
MS.gene91598.t1 AT3G29680 35.518 473 273 10 1 471 1 443 7.19e-78 251
MS.gene91598.t1 AT1G03940 33.265 487 274 17 9 475 9 464 8.51e-70 230
MS.gene91598.t1 AT1G03495 33.604 369 204 14 1 355 1 342 1.73e-47 167
MS.gene91598.t1 AT5G23940 25.828 453 282 20 29 465 25 439 8.33e-23 101
MS.gene91598.t1 AT5G57840 24.224 483 304 21 8 475 3 438 1.82e-20 94.4
MS.gene91598.t1 AT3G29720 32.558 215 113 7 105 311 26 216 4.84e-19 86.3
MS.gene91598.t1 AT5G41040 23.877 423 266 18 62 473 79 456 8.20e-19 89.4
MS.gene91598.t1 AT5G41040 23.877 423 266 18 62 473 63 440 8.67e-19 89.0
MS.gene91598.t1 AT3G48720 22.145 429 286 15 42 467 41 424 3.78e-15 77.8
MS.gene91598.t1 AT2G39980 23.361 488 316 20 7 464 7 466 5.14e-15 77.8
MS.gene91598.t1 AT3G29690 29.412 153 100 3 324 475 33 178 5.42e-15 73.6
MS.gene91598.t1 AT5G07850 22.717 449 285 16 1 431 1 405 1.55e-13 73.2
MS.gene91598.t1 AT5G47980 22.819 447 265 16 42 467 38 425 1.65e-13 72.8
MS.gene91598.t1 AT5G67160 23.469 392 228 13 56 427 43 382 4.26e-13 71.6
MS.gene91598.t1 AT5G67150 22.500 480 314 17 15 478 11 448 1.28e-12 70.1
MS.gene91598.t1 AT1G65450 22.967 492 311 19 1 476 11 450 9.37e-12 67.4
MS.gene91598.t1 AT5G63560 22.251 391 249 16 42 427 40 380 1.51e-11 66.6
MS.gene91598.t1 AT3G47170 22.876 306 196 10 48 345 57 330 8.56e-11 64.3

Find 96 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAATACCAAATAACTATTT+TGG 0.140915 7.4:-84855051 MS.gene91598:CDS
AGCAAATGGTATTATCAAAA+TGG 0.196221 7.4:-84855245 MS.gene91598:CDS
CAATTTCCAAAATAGTTATT+TGG 0.203936 7.4:+84855045 None:intergenic
CGAACTGAGTCTTCGAGTTT+TGG 0.230080 7.4:+84855309 None:intergenic
GAGAGTGAAGATTTGAGAAT+TGG 0.258335 7.4:+84855864 None:intergenic
TCACTTTCTTCGACGTATTA+TGG 0.286027 7.4:-84855974 MS.gene91598:CDS
TAACCTTGATCTGCAATCTA+TGG 0.291297 7.4:+84855080 None:intergenic
TTTGGGGTATGTTGGATTAT+TGG 0.292562 7.4:+84855777 None:intergenic
CTTTAGCTGGTAAGATCATT+TGG 0.302896 7.4:-84855818 MS.gene91598:CDS
CCAAAATAGTTATTTGGTAT+TGG 0.315354 7.4:+84855051 None:intergenic
AATGTAACACCAAGTTCATT+TGG 0.336977 7.4:+84855417 None:intergenic
GCAAGGAAACACCATCATTT+GGG 0.342122 7.4:+84855760 None:intergenic
GAGAGGGATTGTTGTGGTTT+CGG 0.344541 7.4:+84855995 None:intergenic
AGGATGTTCCCAAAGGAAAA+AGG 0.350929 7.4:-84855366 MS.gene91598:CDS
AGCAAGGAAACACCATCATT+TGG 0.361755 7.4:+84855759 None:intergenic
CTTCAAGGATCTATTAATGA+TGG 0.372903 7.4:-84854907 MS.gene91598:CDS
CAAAGTTGGTGATTCTTCAT+TGG 0.372965 7.4:+84855485 None:intergenic
GGTGTTTATGAGACAGATTT+TGG 0.373033 7.4:-84854814 MS.gene91598:CDS
ATAAATATGATAAATGATAA+TGG 0.373774 7.4:-84854934 MS.gene91598:CDS
GAACAACATTGCAGTGTTCA+TGG 0.374820 7.4:+84856030 None:intergenic
AGTTAGAACCGCTATTCAAC+AGG 0.375697 7.4:-84855455 MS.gene91598:CDS
TGTTACGCAAAAGCGATTCA+TGG 0.376237 7.4:-84855141 MS.gene91598:CDS
AACCATGTTCATGAAAGCTT+GGG 0.389836 7.4:-84855535 MS.gene91598:CDS
ACACACCATGCTATTCTTGA+TGG 0.393789 7.4:-84855567 MS.gene91598:CDS
GAAGGTTGTGATTGTGAACT+TGG 0.400171 7.4:+84855909 None:intergenic
CAACCATGTTCATGAAAGCT+TGG 0.403241 7.4:-84855536 MS.gene91598:CDS
AATGATAATGGTGTTCTTCA+AGG 0.408022 7.4:-84854922 MS.gene91598:CDS
TCTTTGATAAATGAGGGATT+TGG 0.413699 7.4:-84854868 MS.gene91598:CDS
CACCATCATTTGGGGTATGT+TGG 0.420080 7.4:+84855769 None:intergenic
GATTGTTGTGGTTTCGGTTG+AGG 0.422598 7.4:+84856001 None:intergenic
TAGAAGAAAACACGTTCAAC+TGG 0.423202 7.4:+84855939 None:intergenic
GATTATTGGTTGTTGACATT+CGG 0.423703 7.4:+84855791 None:intergenic
TAGAGGTTTAACCATTGGTT+TGG 0.430246 7.4:-84854752 MS.gene91598:CDS
ATATCATTCTTTGATAAATG+AGG 0.432734 7.4:-84854875 MS.gene91598:CDS
CTTCGACGTATTATGGCTAA+AGG 0.435538 7.4:-84855967 MS.gene91598:CDS
GCAGAGAGCAAAGATGAAAA+TGG 0.440966 7.4:-84854730 MS.gene91598:CDS
TGTTCTTAATAAGCATGTAA+TGG 0.445189 7.4:-84854689 MS.gene91598:CDS
AACCTTGATCTGCAATCTAT+GGG 0.445561 7.4:+84855081 None:intergenic
ATGTAACACCAAGTTCATTT+GGG 0.446809 7.4:+84855418 None:intergenic
GCAAATGGTATTATCAAAAT+GGG 0.448722 7.4:-84855244 MS.gene91598:CDS
TCCCCATAGATTGCAGATCA+AGG 0.465293 7.4:-84855083 MS.gene91598:CDS
ATCAAAGACCCAAATGAACT+TGG 0.475521 7.4:-84855426 MS.gene91598:CDS
GAACACTTTCTCCCTTTAGC+TGG 0.481894 7.4:-84855831 MS.gene91598:CDS
CAAATGATCTTACCAGCTAA+AGG 0.483763 7.4:+84855819 None:intergenic
GTTGTGGTTTCGGTTGAGGA+TGG 0.488903 7.4:+84856005 None:intergenic
TTTGAACAAGATAAAGCAAA+TGG 0.489572 7.4:-84855259 MS.gene91598:CDS
AAATAGTTATTTGGTATTGG+AGG 0.490971 7.4:+84855054 None:intergenic
TATCATTCTTTGATAAATGA+GGG 0.498356 7.4:-84854874 MS.gene91598:CDS
GAGATGGTGTCGGTGGATAG+AGG 0.498924 7.4:-84854769 MS.gene91598:CDS
AAGAATAGCATGGTGTGTAC+TGG 0.504553 7.4:+84855572 None:intergenic
GTGGATAGAGGTTTAACCAT+TGG 0.504771 7.4:-84854757 MS.gene91598:CDS
AAATGATCTTACCAGCTAAA+GGG 0.506511 7.4:+84855820 None:intergenic
TTCAAGGATCTATTAATGAT+GGG 0.507647 7.4:-84854906 MS.gene91598:CDS
TGGTTGGGGAAGGCCAGAAA+AGG 0.508957 7.4:-84854794 MS.gene91598:CDS
AGCGATTCATGGAGCTGCTA+AGG 0.521717 7.4:-84855130 MS.gene91598:CDS
ACAAAGGTTGATAAAGATTG+TGG 0.527106 7.4:+84855189 None:intergenic
ATCCAACATACCCCAAATGA+TGG 0.527410 7.4:-84855771 MS.gene91598:CDS
TTGAATAGCGGTTCTAACTC+AGG 0.536664 7.4:+84855459 None:intergenic
GAAAATGGTGGGGTTGAAGT+TGG 0.538497 7.4:-84854715 MS.gene91598:CDS
TTGGGGAAGGCCAGAAAAGG+TGG 0.564569 7.4:-84854791 MS.gene91598:CDS
AGCCCAAGCTTTCATGAACA+TGG 0.566822 7.4:+84855533 None:intergenic
TTTGCTCTCTGCCAAACCAA+TGG 0.567957 7.4:+84854741 None:intergenic
TCTAACTCAGGTAACAAAGT+TGG 0.570044 7.4:+84855471 None:intergenic
CTAAAGGGAGAAAGTGTTCG+AGG 0.579709 7.4:+84855835 None:intergenic
TTGTGATTGTGAACTTGGGA+GGG 0.581429 7.4:+84855914 None:intergenic
AAGGCCAGAAAAGGTGGAGA+TGG 0.582944 7.4:-84854785 MS.gene91598:CDS
TCTTCTTCTATAAAGTCCGA+TGG 0.583652 7.4:+84854994 None:intergenic
ATTGGTTGTTGACATTCGGA+AGG 0.585284 7.4:+84855795 None:intergenic
AGAGCAAAGATGAAAATGGT+GGG 0.589440 7.4:-84854726 MS.gene91598:CDS
GTGGTTTCGGTTGAGGATGG+AGG 0.599427 7.4:+84856008 None:intergenic
ATGATTTCCCTGTTGAATAG+CGG 0.599659 7.4:+84855447 None:intergenic
TCGGACTTTATAGAAGAAGA+TGG 0.601034 7.4:-84854991 MS.gene91598:CDS
GTTGCACTGAGATCTTATCC+AGG 0.604812 7.4:-84855386 MS.gene91598:CDS
GTTGGACTCTGCAATGAGCA+AGG 0.605899 7.4:+84855743 None:intergenic
AAGGTTGTGATTGTGAACTT+GGG 0.610997 7.4:+84855910 None:intergenic
TTGCTTAATTGACTGTAGAG+AGG 0.620098 7.4:-84854635 MS.gene91598:CDS
GAGAGCAAAGATGAAAATGG+TGG 0.623496 7.4:-84854727 MS.gene91598:CDS
GACTTGATCGAAATCAACGT+TGG 0.624305 7.4:+84855725 None:intergenic
GAAAGTGAGAGGGATTGTTG+TGG 0.628565 7.4:+84855989 None:intergenic
GAATCTGCGGATTCCAAATG+CGG 0.634455 7.4:+84855657 None:intergenic
AATACGTCGAAGAAAGTGAG+AGG 0.635541 7.4:+84855978 None:intergenic
TTCAAGCGTGAGTTTGAACG+TGG 0.637190 7.4:+84855284 None:intergenic
AAAGGTGGAGATGGTGTCGG+TGG 0.637321 7.4:-84854776 MS.gene91598:CDS
AGAAAAGGTGGAGATGGTGT+CGG 0.642346 7.4:-84854779 MS.gene91598:CDS
AATAGAAGCAAAAGAATCTG+CGG 0.642981 7.4:+84855644 None:intergenic
CTTATCCAGGATGTTCCCAA+AGG 0.644668 7.4:-84855373 MS.gene91598:CDS
ATGGTGTGTACTGGTTCCAA+TGG 0.651004 7.4:+84855581 None:intergenic
TAAAGGGAGAAAGTGTTCGA+GGG 0.656830 7.4:+84855836 None:intergenic
CAAGGAAACACCATCATTTG+GGG 0.665742 7.4:+84855761 None:intergenic
GTTAGAACCGCTATTCAACA+GGG 0.672583 7.4:-84855454 MS.gene91598:CDS
ATACGTCGAAGAAAGTGAGA+GGG 0.677965 7.4:+84855979 None:intergenic
CGAGACAAAGAAGCTTCATG+AGG 0.679550 7.4:+84855690 None:intergenic
GTTGTGATTGTGAACTTGGG+AGG 0.690589 7.4:+84855913 None:intergenic
ACCTTGATCTGCAATCTATG+GGG 0.692544 7.4:+84855082 None:intergenic
GAGCAAAGATGAAAATGGTG+GGG 0.708424 7.4:-84854725 MS.gene91598:CDS
ATAAGCACAAGTGAGAACAA+AGG 0.714865 7.4:+84855173 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATATGATAAATGATAA+TGG - chr7.4:84855746-84855765 MS.gene91598:CDS 10.0%
!! ATATCATTCTTTGATAAATG+AGG - chr7.4:84855805-84855824 MS.gene91598:CDS 20.0%
!! CAATTTCCAAAATAGTTATT+TGG + chr7.4:84855638-84855657 None:intergenic 20.0%
!! TATCATTCTTTGATAAATGA+GGG - chr7.4:84855806-84855825 MS.gene91598:CDS 20.0%
!!! AAAAACCACTTTTTTGAAAA+AGG + chr7.4:84855079-84855098 None:intergenic 20.0%
!!! AAAACCACTTTTTTGAAAAA+GGG + chr7.4:84855078-84855097 None:intergenic 20.0%
!!! TTTTATTGATACAAAACCAT+CGG - chr7.4:84855670-84855689 MS.gene91598:CDS 20.0%
! AGCAAATGGTATTATCAAAA+TGG - chr7.4:84855435-84855454 MS.gene91598:CDS 25.0%
! CCAAAATAGTTATTTGGTAT+TGG + chr7.4:84855632-84855651 None:intergenic 25.0%
! GCAAATGGTATTATCAAAAT+GGG - chr7.4:84855436-84855455 MS.gene91598:CDS 25.0%
! TGTTCTTAATAAGCATGTAA+TGG - chr7.4:84855991-84856010 MS.gene91598:CDS 25.0%
! TTCAAGGATCTATTAATGAT+GGG - chr7.4:84855774-84855793 MS.gene91598:CDS 25.0%
! TTTGAACAAGATAAAGCAAA+TGG - chr7.4:84855421-84855440 MS.gene91598:CDS 25.0%
!! AAATAGTTATTTGGTATTGG+AGG + chr7.4:84855629-84855648 None:intergenic 25.0%
!! CCAATACCAAATAACTATTT+TGG - chr7.4:84855629-84855648 MS.gene91598:CDS 25.0%
!!! ACTATTTTGGAAATTGTGTT+TGG - chr7.4:84855642-84855661 MS.gene91598:CDS 25.0%
!!! ATAACCCTTTTTCAAAAAAG+TGG - chr7.4:84855071-84855090 MS.gene91598:CDS 25.0%
!!! CTATTTTGGAAATTGTGTTT+GGG - chr7.4:84855643-84855662 MS.gene91598:CDS 25.0%
!!! TATTTTGGAAATTGTGTTTG+GGG - chr7.4:84855644-84855663 MS.gene91598:CDS 25.0%
!!! TTTAGCACTTTCTTTTTTGA+AGG - chr7.4:84856019-84856038 MS.gene91598:CDS 25.0%
AAATGATCTTACCAGCTAAA+GGG + chr7.4:84854863-84854882 None:intergenic 30.0%
AATAGAAGCAAAAGAATCTG+CGG + chr7.4:84855039-84855058 None:intergenic 30.0%
AATGTAACACCAAGTTCATT+TGG + chr7.4:84855266-84855285 None:intergenic 30.0%
ATGTAACACCAAGTTCATTT+GGG + chr7.4:84855265-84855284 None:intergenic 30.0%
CTTCAAGGATCTATTAATGA+TGG - chr7.4:84855773-84855792 MS.gene91598:CDS 30.0%
TCTTTGATAAATGAGGGATT+TGG - chr7.4:84855812-84855831 MS.gene91598:CDS 30.0%
! ATTTTCCATCAAGAATAGCA+TGG + chr7.4:84855121-84855140 None:intergenic 30.0%
! CAAAAAAGTGGTTTTTCCAT+TGG - chr7.4:84855083-84855102 MS.gene91598:CDS 30.0%
! GATTATTGGTTGTTGACATT+CGG + chr7.4:84854892-84854911 None:intergenic 30.0%
! TGAGAATTGGAACAAGTTTT+TGG + chr7.4:84854806-84854825 None:intergenic 30.0%
!! AATGATAATGGTGTTCTTCA+AGG - chr7.4:84855758-84855777 MS.gene91598:CDS 30.0%
!! ACAAAGGTTGATAAAGATTG+TGG + chr7.4:84855494-84855513 None:intergenic 30.0%
!! AGTTTTTGGAAGAAGAAAGA+AGG + chr7.4:84854792-84854811 None:intergenic 30.0%
!! ATTTTGGAAATTGTGTTTGG+GGG - chr7.4:84855645-84855664 MS.gene91598:CDS 30.0%
!! CAAAAAAGAAAGTGCTAAAG+AGG + chr7.4:84856018-84856037 None:intergenic 30.0%
!! TTTATGAGACAGATTTTGGT+TGG - chr7.4:84855870-84855889 MS.gene91598:CDS 30.0%
!!! TTATGAGACAGATTTTGGTT+GGG - chr7.4:84855871-84855890 MS.gene91598:CDS 30.0%
AACCATGTTCATGAAAGCTT+GGG - chr7.4:84855145-84855164 MS.gene91598:CDS 35.0%
AACCTTGATCTGCAATCTAT+GGG + chr7.4:84855602-84855621 None:intergenic 35.0%
AAGGTTGTGATTGTGAACTT+GGG + chr7.4:84854773-84854792 None:intergenic 35.0%
AGAGCAAAGATGAAAATGGT+GGG - chr7.4:84855954-84855973 MS.gene91598:CDS 35.0%
ATAAGCACAAGTGAGAACAA+AGG + chr7.4:84855510-84855529 None:intergenic 35.0%
ATCAAAGACCCAAATGAACT+TGG - chr7.4:84855254-84855273 MS.gene91598:CDS 35.0%
ATGATTTCCCTGTTGAATAG+CGG + chr7.4:84855236-84855255 None:intergenic 35.0%
CAAATGATCTTACCAGCTAA+AGG + chr7.4:84854864-84854883 None:intergenic 35.0%
CTTTAGCTGGTAAGATCATT+TGG - chr7.4:84854862-84854881 MS.gene91598:CDS 35.0%
GAGAGTGAAGATTTGAGAAT+TGG + chr7.4:84854819-84854838 None:intergenic 35.0%
TAACCTTGATCTGCAATCTA+TGG + chr7.4:84855603-84855622 None:intergenic 35.0%
TAGAAGAAAACACGTTCAAC+TGG + chr7.4:84854744-84854763 None:intergenic 35.0%
TCACTTTCTTCGACGTATTA+TGG - chr7.4:84854706-84854725 MS.gene91598:CDS 35.0%
TCGGACTTTATAGAAGAAGA+TGG - chr7.4:84855689-84855708 MS.gene91598:CDS 35.0%
TCTAACTCAGGTAACAAAGT+TGG + chr7.4:84855212-84855231 None:intergenic 35.0%
TCTTCTTCTATAAAGTCCGA+TGG + chr7.4:84855689-84855708 None:intergenic 35.0%
TTGCTTAATTGACTGTAGAG+AGG - chr7.4:84856045-84856064 MS.gene91598:CDS 35.0%
! GGTGTTTATGAGACAGATTT+TGG - chr7.4:84855866-84855885 MS.gene91598:CDS 35.0%
! GTTCGTTTCCTTTTTCCTTT+GGG + chr7.4:84855325-84855344 None:intergenic 35.0%
! TAGAGGTTTAACCATTGGTT+TGG - chr7.4:84855928-84855947 MS.gene91598:CDS 35.0%
! TCGTTCTTTTATACCGCATT+TGG - chr7.4:84855010-84855029 MS.gene91598:CDS 35.0%
! TGTTCGTTTCCTTTTTCCTT+TGG + chr7.4:84855326-84855345 None:intergenic 35.0%
! TTTGGGGTATGTTGGATTAT+TGG + chr7.4:84854906-84854925 None:intergenic 35.0%
!! CAAAGTTGGTGATTCTTCAT+TGG + chr7.4:84855198-84855217 None:intergenic 35.0%
!!! AATGAGGGATTTGGTGTTTT+TGG - chr7.4:84855821-84855840 MS.gene91598:CDS 35.0%
!!! ATTTGGTGTTTTTGGAGTTG+GGG - chr7.4:84855829-84855848 MS.gene91598:CDS 35.0%
!!! GATTTGGTGTTTTTGGAGTT+GGG - chr7.4:84855828-84855847 MS.gene91598:CDS 35.0%
!!! TATGAGACAGATTTTGGTTG+GGG - chr7.4:84855872-84855891 MS.gene91598:CDS 35.0%
AAGAATAGCATGGTGTGTAC+TGG + chr7.4:84855111-84855130 None:intergenic 40.0%
AATACGTCGAAGAAAGTGAG+AGG + chr7.4:84854705-84854724 None:intergenic 40.0%
ACACACCATGCTATTCTTGA+TGG - chr7.4:84855113-84855132 MS.gene91598:CDS 40.0%
ACCTTGATCTGCAATCTATG+GGG + chr7.4:84855601-84855620 None:intergenic 40.0%
AGCAAGGAAACACCATCATT+TGG + chr7.4:84854924-84854943 None:intergenic 40.0%
AGGATGTTCCCAAAGGAAAA+AGG - chr7.4:84855314-84855333 MS.gene91598:CDS 40.0%
AGTTAGAACCGCTATTCAAC+AGG - chr7.4:84855225-84855244 MS.gene91598:CDS 40.0%
ATACGTCGAAGAAAGTGAGA+GGG + chr7.4:84854704-84854723 None:intergenic 40.0%
ATCCAACATACCCCAAATGA+TGG - chr7.4:84854909-84854928 MS.gene91598:CDS 40.0%
ATTGGTTGTTGACATTCGGA+AGG + chr7.4:84854888-84854907 None:intergenic 40.0%
CAACCATGTTCATGAAAGCT+TGG - chr7.4:84855144-84855163 MS.gene91598:CDS 40.0%
CAAGGAAACACCATCATTTG+GGG + chr7.4:84854922-84854941 None:intergenic 40.0%
CTTCGACGTATTATGGCTAA+AGG - chr7.4:84854713-84854732 MS.gene91598:CDS 40.0%
GAACAACATTGCAGTGTTCA+TGG + chr7.4:84854653-84854672 None:intergenic 40.0%
GAAGGTTGTGATTGTGAACT+TGG + chr7.4:84854774-84854793 None:intergenic 40.0%
GACTTGATCGAAATCAACGT+TGG + chr7.4:84854958-84854977 None:intergenic 40.0%
GAGAGCAAAGATGAAAATGG+TGG - chr7.4:84855953-84855972 MS.gene91598:CDS 40.0%
GAGCAAAGATGAAAATGGTG+GGG - chr7.4:84855955-84855974 MS.gene91598:CDS 40.0%
GCAAGGAAACACCATCATTT+GGG + chr7.4:84854923-84854942 None:intergenic 40.0%
GCAGAGAGCAAAGATGAAAA+TGG - chr7.4:84855950-84855969 MS.gene91598:CDS 40.0%
GTGGATAGAGGTTTAACCAT+TGG - chr7.4:84855923-84855942 MS.gene91598:CDS 40.0%
GTTAGAACCGCTATTCAACA+GGG - chr7.4:84855226-84855245 MS.gene91598:CDS 40.0%
TGTTACGCAAAAGCGATTCA+TGG - chr7.4:84855539-84855558 MS.gene91598:CDS 40.0%
TTGTGATTGTGAACTTGGGA+GGG + chr7.4:84854769-84854788 None:intergenic 40.0%
! TTGAATAGCGGTTCTAACTC+AGG + chr7.4:84855224-84855243 None:intergenic 40.0%
!! TAAAGGGAGAAAGTGTTCGA+GGG + chr7.4:84854847-84854866 None:intergenic 40.0%
!! TTTTTCCTTTGGGAACATCC+TGG + chr7.4:84855315-84855334 None:intergenic 40.0%
!!! GGATTTGGTGTTTTTGGAGT+TGG - chr7.4:84855827-84855846 MS.gene91598:CDS 40.0%
!!! TTTGGTGTTTTTGGAGTTGG+GGG - chr7.4:84855830-84855849 MS.gene91598:CDS 40.0%
AGAAAAGGTGGAGATGGTGT+CGG - chr7.4:84855901-84855920 MS.gene91598:CDS 45.0%
AGCCCAAGCTTTCATGAACA+TGG + chr7.4:84855150-84855169 None:intergenic 45.0%
CGAGACAAAGAAGCTTCATG+AGG + chr7.4:84854993-84855012 None:intergenic 45.0%
CTTATCCAGGATGTTCCCAA+AGG - chr7.4:84855307-84855326 MS.gene91598:CDS 45.0%
GAAAGTGAGAGGGATTGTTG+TGG + chr7.4:84854694-84854713 None:intergenic 45.0%
GAACACTTTCTCCCTTTAGC+TGG - chr7.4:84854849-84854868 MS.gene91598:CDS 45.0%
GAATCTGCGGATTCCAAATG+CGG + chr7.4:84855026-84855045 None:intergenic 45.0%
GAGAGGGATTGTTGTGGTTT+CGG + chr7.4:84854688-84854707 None:intergenic 45.0%
GATTGTTGTGGTTTCGGTTG+AGG + chr7.4:84854682-84854701 None:intergenic 45.0%
GTTGCACTGAGATCTTATCC+AGG - chr7.4:84855294-84855313 MS.gene91598:CDS 45.0%
GTTGGGGGGTCTAACAAATT+TGG - chr7.4:84855845-84855864 MS.gene91598:CDS 45.0%
GTTGTGATTGTGAACTTGGG+AGG + chr7.4:84854770-84854789 None:intergenic 45.0%
TCCCCATAGATTGCAGATCA+AGG - chr7.4:84855597-84855616 MS.gene91598:CDS 45.0%
TTCAAGCGTGAGTTTGAACG+TGG + chr7.4:84855399-84855418 None:intergenic 45.0%
TTTGCTCTCTGCCAAACCAA+TGG + chr7.4:84855942-84855961 None:intergenic 45.0%
! CACCATCATTTGGGGTATGT+TGG + chr7.4:84854914-84854933 None:intergenic 45.0%
! GAAAATGGTGGGGTTGAAGT+TGG - chr7.4:84855965-84855984 MS.gene91598:CDS 45.0%
!! ATGGTGTGTACTGGTTCCAA+TGG + chr7.4:84855102-84855121 None:intergenic 45.0%
!! CGAACTGAGTCTTCGAGTTT+TGG + chr7.4:84855374-84855393 None:intergenic 45.0%
!! CTAAAGGGAGAAAGTGTTCG+AGG + chr7.4:84854848-84854867 None:intergenic 45.0%
!!! AGACAGATTTTGGTTGGGGA+AGG - chr7.4:84855876-84855895 MS.gene91598:CDS 45.0%
!!! TTGGTGTTTTTGGAGTTGGG+GGG - chr7.4:84855831-84855850 MS.gene91598:CDS 45.0%
AAGGCCAGAAAAGGTGGAGA+TGG - chr7.4:84855895-84855914 MS.gene91598:CDS 50.0%
AGCGATTCATGGAGCTGCTA+AGG - chr7.4:84855550-84855569 MS.gene91598:CDS 50.0%
GTTGGACTCTGCAATGAGCA+AGG + chr7.4:84854940-84854959 None:intergenic 50.0%
GTTGTGGTTTCGGTTGAGGA+TGG + chr7.4:84854678-84854697 None:intergenic 50.0%
! GACACCATCTCCACCTTTTC+TGG + chr7.4:84855902-84855921 None:intergenic 50.0%
GTGGTTTCGGTTGAGGATGG+AGG + chr7.4:84854675-84854694 None:intergenic 55.0%
TGGTTGGGGAAGGCCAGAAA+AGG - chr7.4:84855886-84855905 MS.gene91598:CDS 55.0%
TTGGGGAAGGCCAGAAAAGG+TGG - chr7.4:84855889-84855908 MS.gene91598:CDS 55.0%
! AAAGGTGGAGATGGTGTCGG+TGG - chr7.4:84855904-84855923 MS.gene91598:CDS 55.0%
!! GAGATGGTGTCGGTGGATAG+AGG - chr7.4:84855911-84855930 MS.gene91598:CDS 55.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 84854621 84856081 84854621 ID=MS.gene91598
chr7.4 mRNA 84854621 84856081 84854621 ID=MS.gene91598.t1;Parent=MS.gene91598
chr7.4 exon 84854621 84856081 84854621 ID=MS.gene91598.t1.exon1;Parent=MS.gene91598.t1
chr7.4 CDS 84854621 84856081 84854621 ID=cds.MS.gene91598.t1;Parent=MS.gene91598.t1
Gene Sequence

>MS.gene91598

ATGGCTTTAAACAACAACAACATCAAAATCCATGAACACTGCAATGTTGTTCCTCCATCCTCAACCGAAACCACAACAATCCCTCTCACTTTCTTCGACGTATTATGGCTAAAGGTATATCCAGTTGAACGTGTTTTCTTCTACACCCTCCCAAGTTCACAATCACAACCTTCTTTCTTCTTCCAAAAACTTGTTCCAATTCTCAAATCTTCACTCTCTTTAACCCTCGAACACTTTCTCCCTTTAGCTGGTAAGATCATTTGGCCTTCCGAATGTCAACAACCAATAATCCAACATACCCCAAATGATGGTGTTTCCTTGCTCATTGCAGAGTCCAACGTTGATTTCGATCAAGTCATTGAAAACTCACCTCATGAAGCTTCTTTGTCTCGTTCTTTTATACCGCATTTGGAATCCGCAGATTCTTTTGCTTCTATTATTTCAATTCAGATAACCCTTTTTCAAAAAAGTGGTTTTTCCATTGGAACCAGTACACACCATGCTATTCTTGATGGAAAATCTTCAACCATGTTCATGAAAGCTTGGGCTTATTTATGCAAAAAAATAATTGAAACCAATGAAGAATCACCAACTTTGTTACCTGAGTTAGAACCGCTATTCAACAGGGAAATCATCAAAGACCCAAATGAACTTGGTGTTACATTCACAAATAGTTGCACTGAGATCTTATCCAGGATGTTCCCAAAGGAAAAAGGAAACGAACATAGCTTAAATATTTTACACTTTGAACCAAAACTCGAAGACTCAGTTCGTGCCACGTTCAAACTCACGCTTGAAAATTTGAACAAGATAAAGCAAATGGTATTATCAAAATGGGAAGTTGTTGATAAAAATGAATCAAATTCAAAGCCACAATCTTTATCAACCTTTGTTCTCACTTGTGCTTATTCACTCGTTTGTTACGCAAAAGCGATTCATGGAGCTGCTAAGGAGAAAGAGAAGTTTTGTTTTGCTTTCCCCATAGATTGCAGATCAAGGTTAGAACCTCCAATACCAAATAACTATTTTGGAAATTGTGTTTGGGGGCATTTTATTGATACAAAACCATCGGACTTTATAGAAGAAGATGGTGTTTATTTAGTTGCTAAGAGTATTCATGAGAAAATAAATATGATAAATGATAATGGTGTTCTTCAAGGATCTATTAATGATGGGTTTGACAAATATCATTCTTTGATAAATGAGGGATTTGGTGTTTTTGGAGTTGGGGGGTCTAACAAATTTGGTGTTTATGAGACAGATTTTGGTTGGGGAAGGCCAGAAAAGGTGGAGATGGTGTCGGTGGATAGAGGTTTAACCATTGGTTTGGCAGAGAGCAAAGATGAAAATGGTGGGGTTGAAGTTGGTCTTGTTCTTAATAAGCATGTAATGGACCTCTTTAGCACTTTCTTTTTTGAAGGACTTTGCTTAATTGACTGTAGAGAGGAAGCGCAATGTTGA
Protein sequence

>MS.gene91598.t1

MALNNNNIKIHEHCNVVPPSSTETTTIPLTFFDVLWLKVYPVERVFFYTLPSSQSQPSFFFQKLVPILKSSLSLTLEHFLPLAGKIIWPSECQQPIIQHTPNDGVSLLIAESNVDFDQVIENSPHEASLSRSFIPHLESADSFASIISIQITLFQKSGFSIGTSTHHAILDGKSSTMFMKAWAYLCKKIIETNEESPTLLPELEPLFNREIIKDPNELGVTFTNSCTEILSRMFPKEKGNEHSLNILHFEPKLEDSVRATFKLTLENLNKIKQMVLSKWEVVDKNESNSKPQSLSTFVLTCAYSLVCYAKAIHGAAKEKEKFCFAFPIDCRSRLEPPIPNNYFGNCVWGHFIDTKPSDFIEEDGVYLVAKSIHEKINMINDNGVLQGSINDGFDKYHSLINEGFGVFGVGGSNKFGVYETDFGWGRPEKVEMVSVDRGLTIGLAESKDENGGVEVGLVLNKHVMDLFSTFFFEGLCLIDCREEAQC