AlfalfaGEDB | MS.gene021905

M. sativa cultivar XinJiangDaYe / MS.gene021905

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016740 GO:0016746 GO:0016747
PFAM: PF02458.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene021905.t1	XP_013447578.1	80.4	484	90	4	1	480	1	483	5.00E-219	770.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene021905.t1	Q9LJB4	37.8	484	258	12	1	477	1	448	5.6e-78	293.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene021905.t1	B4Y0U1	80.4	484	90	4	1	480	1	483	3.6e-219	770.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene44643	MS.gene021905	PPI
MS.gene028999	MS.gene021905	PPI
MS.gene50379	MS.gene021905	PPI
MS.gene021905	MS.gene043350	PPI
MS.gene021905	MS.gene011716	PPI
MS.gene31545	MS.gene021905	PPI
MS.gene066701	MS.gene021905	PPI
MS.gene44633	MS.gene021905	PPI
MS.gene021915	MS.gene021905	PPI
MS.gene91614	MS.gene021905	PPI
MS.gene91612	MS.gene021905	PPI
MS.gene50382	MS.gene021905	PPI
MS.gene91597	MS.gene021905	PPI
MS.gene021916	MS.gene021905	PPI
MS.gene021905	MS.gene021917	PPI
MS.gene31546	MS.gene021905	PPI
MS.gene35741	MS.gene021905	PPI
MS.gene064101	MS.gene021905	PPI
MS.gene021914	MS.gene021905	PPI
MS.gene90496	MS.gene021905	PPI
MS.gene91598	MS.gene021905	PPI
MS.gene021910	MS.gene021905	PPI
MS.gene028996	MS.gene021905	PPI
MS.gene000763	MS.gene021905	PPI
MS.gene28251	MS.gene021905	PPI
MS.gene91613	MS.gene021905	PPI
MS.gene011801	MS.gene021905	PPI
MS.gene87496	MS.gene021905	PPI
MS.gene000764	MS.gene021905	PPI
MS.gene91615	MS.gene021905	PPI
MS.gene48582	MS.gene021905	PPI
MS.gene56799	MS.gene021905	PPI
MS.gene38977	MS.gene021905	PPI
MS.gene90491	MS.gene021905	PPI
MS.gene021905	MS.gene91595	PPI
MS.gene87532	MS.gene021905	PPI
MS.gene99223	MS.gene021905	PPI
MS.gene46265	MS.gene021905	PPI
MS.gene90492	MS.gene021905	PPI
MS.gene063868	MS.gene021905	PPI
MS.gene066658	MS.gene021905	PPI
MS.gene35740	MS.gene021905	PPI
MS.gene48581	MS.gene021905	PPI
MS.gene48585	MS.gene021905	PPI
MS.gene87191	MS.gene021905	PPI
MS.gene021917	MS.gene021905	PPI
MS.gene011837	MS.gene021905	PPI
MS.gene066634	MS.gene021905	PPI
MS.gene043346	MS.gene021905	PPI
MS.gene91599	MS.gene021905	PPI
MS.gene56797	MS.gene021905	PPI
MS.gene021905	MS.gene064101	PPI
MS.gene021905	MS.gene070270	PPI
MS.gene016532	MS.gene021905	PPI
MS.gene49853	MS.gene021905	PPI
MS.gene063865	MS.gene021905	PPI
MS.gene007831	MS.gene021905	PPI
MS.gene021909	MS.gene021905	PPI
MS.gene011799	MS.gene021905	PPI
MS.gene44642	MS.gene021905	PPI
MS.gene021905	MS.gene56797	PPI
MS.gene56796	MS.gene021905	PPI
MS.gene021905	MS.gene99218	PPI
MS.gene069767	MS.gene021905	PPI
MS.gene004218	MS.gene021905	PPI
MS.gene44644	MS.gene021905	PPI
MS.gene35745	MS.gene021905	PPI
MS.gene011796	MS.gene021905	PPI
MS.gene87577	MS.gene021905	PPI
MS.gene87537	MS.gene021905	PPI
MS.gene99222	MS.gene021905	PPI
MS.gene02573	MS.gene021905	PPI
MS.gene60208	MS.gene021905	PPI
MS.gene90493	MS.gene021905	PPI
MS.gene032349	MS.gene021905	PPI
MS.gene013787	MS.gene021905	PPI
MS.gene066656	MS.gene021905	PPI
MS.gene021905	MS.gene016477	PPI
MS.gene021906	MS.gene021905	PPI
MS.gene91596	MS.gene021905	PPI
MS.gene31542	MS.gene021905	PPI
MS.gene44647	MS.gene021905	PPI
MS.gene066660	MS.gene021905	PPI
MS.gene31540	MS.gene021905	PPI
MS.gene44640	MS.gene021905	PPI
MS.gene021913	MS.gene021905	PPI
MS.gene50420	MS.gene021905	PPI
MS.gene043348	MS.gene021905	PPI
MS.gene87536	MS.gene021905	PPI
MS.gene99221	MS.gene021905	PPI
MS.gene03202	MS.gene021905	PPI
MS.gene50384	MS.gene021905	PPI
MS.gene012360	MS.gene021905	PPI
MS.gene028998	MS.gene021905	PPI
MS.gene016477	MS.gene021905	PPI
MS.gene91609	MS.gene021905	PPI
MS.gene99218	MS.gene021905	PPI
MS.gene50355	MS.gene021905	PPI
MS.gene011798	MS.gene021905	PPI
MS.gene91611	MS.gene021905	PPI
MS.gene48584	MS.gene021905	PPI
MS.gene50356	MS.gene021905	PPI
MS.gene021904	MS.gene021905	PPI
MS.gene073299	MS.gene021905	PPI
MS.gene021905	MS.gene87496	PPI
MS.gene021905	MS.gene070019	PPI
MS.gene32261	MS.gene021905	PPI
MS.gene44630	MS.gene021905	PPI
MS.gene99219	MS.gene021905	PPI
MS.gene96337	MS.gene021905	PPI
MS.gene043352	MS.gene021905	PPI
MS.gene91595	MS.gene021905	PPI
MS.gene070019	MS.gene021905	PPI
MS.gene021905	MS.gene028994	PPI
MS.gene021905	MS.gene91596	PPI
MS.gene44463	MS.gene021905	PPI
MS.gene56795	MS.gene021905	PPI
MS.gene066657	MS.gene021905	PPI
MS.gene021905	MS.gene56799	PPI
MS.gene56798	MS.gene021905	PPI
MS.gene002544	MS.gene021905	PPI
MS.gene069423	MS.gene021905	PPI
MS.gene78818	MS.gene021905	PPI
MS.gene069422	MS.gene021905	PPI
MS.gene35744	MS.gene021905	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene021905.t1	MTR_7g013850	80.165	484	91	4	1	480	1	483	0.0	775
MS.gene021905.t1	MTR_7g014200	59.298	484	174	12	1	477	1	468	0.0	541
MS.gene021905.t1	MTR_7g014160	58.455	479	182	11	5	477	6	473	0.0	541
MS.gene021905.t1	MTR_7g014610	58.799	483	185	9	1	477	1	475	0.0	521
MS.gene021905.t1	MTR_7g014620	52.711	461	182	7	19	477	6	432	2.60e-150	436
MS.gene021905.t1	MTR_7g014630	57.475	301	115	7	6	301	7	299	4.86e-111	333
MS.gene021905.t1	MTR_6g015315	39.149	470	262	10	9	477	6	452	9.91e-103	315
MS.gene021905.t1	MTR_7g014330	39.958	478	254	15	9	477	3	456	2.52e-101	311
MS.gene021905.t1	MTR_6g015320	36.983	484	268	12	1	477	1	454	1.80e-99	306
MS.gene021905.t1	MTR_7g014230	38.622	479	263	12	9	479	5	460	3.12e-99	306
MS.gene021905.t1	MTR_6g015830	39.588	485	244	14	9	477	9	460	3.20e-99	306
MS.gene021905.t1	MTR_6g015850	38.900	491	252	16	1	477	1	457	5.12e-99	305
MS.gene021905.t1	MTR_7g014250	38.542	480	258	15	9	477	3	456	6.19e-98	302
MS.gene021905.t1	MTR_6g015815	40.481	457	241	11	25	477	31	460	6.03e-97	300
MS.gene021905.t1	MTR_3g028210	35.464	485	270	16	8	477	8	464	4.14e-75	244
MS.gene021905.t1	MTR_7g014180	69.832	179	47	5	1	178	1	173	1.07e-73	230
MS.gene021905.t1	MTR_7g014670	56.477	193	82	2	289	480	71	262	7.64e-69	221
MS.gene021905.t1	MTR_2g089765	32.515	489	281	15	11	477	21	482	1.97e-67	224
MS.gene021905.t1	MTR_7g014360	34.152	407	218	12	78	477	23	386	1.64e-65	216
MS.gene021905.t1	MTR_2g089670	32.397	463	274	14	26	477	43	477	6.15e-62	209
MS.gene021905.t1	MTR_2g089650	28.916	498	298	15	1	477	1	463	3.00e-59	202
MS.gene021905.t1	MTR_2g089735	31.839	446	270	11	42	477	40	461	3.77e-59	202
MS.gene021905.t1	MTR_3g087640	29.779	497	287	11	9	477	10	472	5.09e-59	202
MS.gene021905.t1	MTR_7g015860	31.034	493	280	17	8	477	5	460	2.79e-56	194
MS.gene021905.t1	MTR_2g089755	28.970	466	288	13	28	477	37	475	3.68e-54	189
MS.gene021905.t1	MTR_8g012750	31.210	471	279	15	26	475	24	470	1.83e-53	187
MS.gene021905.t1	MTR_6g015310	30.617	405	208	12	51	455	2	333	6.89e-49	171
MS.gene021905.t1	MTR_7g014310	29.730	333	165	9	130	460	41	306	7.35e-40	146
MS.gene021905.t1	MTR_2g089665	25.164	457	201	14	11	454	7	335	1.04e-25	108
MS.gene021905.t1	MTR_3g435300	30.000	280	164	9	9	276	4	263	6.06e-25	104
MS.gene021905.t1	MTR_4g007540	24.224	483	288	21	8	470	3	427	2.99e-22	99.4
MS.gene021905.t1	MTR_8g075610	24.439	401	232	16	44	429	30	374	3.73e-21	95.9
MS.gene021905.t1	MTR_3g070170	25.664	452	269	17	8	433	12	422	1.06e-18	89.0
MS.gene021905.t1	MTR_3g049150	24.601	439	267	18	63	478	51	448	1.17e-18	88.6
MS.gene021905.t1	MTR_2g064495	24.622	463	271	23	32	475	28	431	3.19e-18	87.0
MS.gene021905.t1	MTR_7g086000	24.449	499	305	19	7	477	3	457	9.74e-18	85.9
MS.gene021905.t1	MTR_2g105330	24.946	461	272	23	32	475	28	431	1.11e-17	85.5
MS.gene021905.t1	MTR_7g014210	38.318	107	64	2	335	439	24	130	1.59e-17	80.5
MS.gene021905.t1	MTR_7g014205	37.615	109	56	2	332	439	17	114	1.01e-16	76.6
MS.gene021905.t1	MTR_3g088420	23.602	483	261	16	17	467	14	420	1.84e-16	81.6
MS.gene021905.t1	MTR_3g026950	23.448	435	270	17	64	473	51	447	2.02e-16	81.6
MS.gene021905.t1	MTR_8g096530	24.599	374	233	10	61	429	53	382	4.91e-16	80.5
MS.gene021905.t1	MTR_4g022800	22.128	470	293	16	17	467	14	429	6.79e-16	80.1
MS.gene021905.t1	MTR_4g127650	23.340	467	269	17	44	477	46	456	8.94e-16	79.7
MS.gene021905.t1	MTR_4g022720	22.766	470	283	17	17	467	14	422	1.97e-15	78.6
MS.gene021905.t1	MTR_4g097090	23.061	477	290	19	26	475	28	454	6.59e-15	77.0
MS.gene021905.t1	MTR_7g085930	23.988	346	230	11	6	349	4	318	7.81e-15	77.0
MS.gene021905.t1	MTR_7g085990	23.841	302	199	9	50	349	42	314	1.65e-14	75.9
MS.gene021905.t1	MTR_3g101970	24.208	442	261	20	8	433	6	389	1.04e-13	73.2
MS.gene021905.t1	MTR_7g014340	53.521	71	26	2	9	75	3	70	5.00e-13	65.5
MS.gene021905.t1	MTR_8g079970	23.158	475	276	18	17	470	15	421	1.22e-12	70.1
MS.gene021905.t1	MTR_4g022825	21.798	445	262	16	28	446	24	408	2.94e-12	68.9
MS.gene021905.t1	MTR_8g079950	23.899	477	270	17	17	470	15	421	4.90e-12	68.2
MS.gene021905.t1	MTR_7g014220	56.604	53	20	1	25	77	15	64	5.79e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene021905.t1	AT3G29590	38.017	484	257	12	1	477	1	448	1.38e-89	281
MS.gene021905.t1	AT5G39080	38.478	473	263	15	1	467	1	451	1.08e-87	276
MS.gene021905.t1	AT3G29670	38.333	480	255	13	8	478	3	450	5.13e-87	274
MS.gene021905.t1	AT5G39050	37.113	485	267	15	6	477	8	467	4.62e-86	272
MS.gene021905.t1	AT3G29635	37.214	481	262	14	8	477	3	454	4.02e-85	270
MS.gene021905.t1	AT5G39090	36.421	475	266	14	6	477	6	447	3.59e-83	264
MS.gene021905.t1	AT1G03940	31.515	495	274	12	9	477	9	464	1.61e-70	232
MS.gene021905.t1	AT5G61160	33.615	473	278	14	8	473	3	446	4.36e-69	228
MS.gene021905.t1	AT3G29680	34.454	476	268	14	6	473	4	443	2.21e-68	226
MS.gene021905.t1	AT1G03495	31.183	372	215	11	1	360	1	343	1.50e-44	160
MS.gene021905.t1	AT5G48930	24.274	482	299	19	8	475	3	432	2.33e-20	93.6
MS.gene021905.t1	AT3G29720	32.870	216	110	10	99	303	10	201	2.08e-16	78.6
MS.gene021905.t1	AT5G57840	23.355	471	290	16	25	477	21	438	1.38e-15	79.3
MS.gene021905.t1	AT3G50280	23.185	427	268	15	19	433	18	396	4.10e-15	77.8
MS.gene021905.t1	AT3G29690	29.801	151	100	2	328	477	33	178	5.02e-15	73.6
MS.gene021905.t1	AT2G39980	23.093	485	335	15	1	466	1	466	1.04e-13	73.6
MS.gene021905.t1	AT5G07850	23.714	447	285	16	1	433	1	405	8.70e-13	70.5
MS.gene021905.t1	AT5G23970	22.822	482	264	20	16	470	11	411	1.02e-12	70.5
MS.gene021905.t1	AT1G28680	21.138	369	247	9	67	432	62	389	2.77e-12	68.9
MS.gene021905.t1	AT5G41040	23.611	432	255	21	61	473	63	438	1.27e-11	67.0
MS.gene021905.t1	AT3G48720	21.823	417	269	14	67	474	61	429	1.31e-11	67.0
MS.gene021905.t1	AT4G31910	22.714	339	226	7	60	389	57	368	1.78e-11	66.6
MS.gene021905.t1	AT5G41040	23.611	432	255	21	61	473	79	454	1.96e-11	66.2

Find 74 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAGACTCTCAAAATTATT+TGG	0.163672	7.1:+81986385	None:intergenic
GAAGGGTGTGATTGTGAATT+TGG	0.182607	7.1:+81986892	None:intergenic
AGAGTCTGTTCATAAATAAT+TGG	0.191926	7.1:-81986372	MS.gene021905:CDS
GACATTGACGTCTAAATATT+TGG	0.199250	7.1:-81985864	MS.gene021905:CDS
TCACTTTCTTTGACTTGTTT+TGG	0.206968	7.1:-81986957	MS.gene021905:CDS
TTGAAAGGTGGTGTTGAATT+TGG	0.210135	7.1:-81985689	MS.gene021905:CDS
GAGAGTGAAGATTTGAGTTT+TGG	0.216722	7.1:+81986847	None:intergenic
GATAATTGGTTTGCAGAATT+AGG	0.221219	7.1:+81986988	None:intergenic
CAGCTAAAGGGAGGAAGTTT+TGG	0.225428	7.1:+81986815	None:intergenic
ACAAAAGTTGACAATTTCTT+AGG	0.235091	7.1:+81986160	None:intergenic
TACTACTCCCCTCTCCAAAA+AGG	0.248355	7.1:+81986414	None:intergenic
ATTGTTGCTAATAAAATTTA+TGG	0.257747	7.1:-81985929	MS.gene021905:CDS
GGAGTATATGAAATTGATTT+TGG	0.266147	7.1:-81985788	MS.gene021905:CDS
TTGAATTTGATGAATGGTTT+TGG	0.270000	7.1:+81986766	None:intergenic
CGAACAGAATCCTTAACTTT+TGG	0.279952	7.1:+81986280	None:intergenic
AAGTGAGAGGGAGAGATAAT+TGG	0.293242	7.1:+81986974	None:intergenic
TCCAAATGGTACATTGTAAA+AGG	0.320135	7.1:-81986202	MS.gene021905:CDS
TGTTTCCTTCGATAAAGTTT+TGG	0.341922	7.1:-81986692	MS.gene021905:CDS
CTTTAGCTGGTAACATCGTT+TGG	0.362068	7.1:-81986801	MS.gene021905:CDS
TCTAAATATTTGGCTATGAT+TGG	0.368222	7.1:-81985854	MS.gene021905:CDS
ATTGATAGAGGATTAACTAT+TGG	0.368461	7.1:-81985731	MS.gene021905:CDS
CTCATTTGAACCAAAAGTTA+AGG	0.369807	7.1:-81986290	MS.gene021905:CDS
TGGACTTATGAAAATGTGAT+AGG	0.386400	7.1:+81986341	None:intergenic
TATTGGTTTAACAGAAAGTA+AGG	0.387767	7.1:-81985714	MS.gene021905:CDS
AAGGAAACTCCGTCATCATT+TGG	0.395044	7.1:+81986742	None:intergenic
ATGGATAAGGGTGTTCTTGA+TGG	0.402404	7.1:-81985893	MS.gene021905:CDS
CCAGTTCCTGAAAACTACTT+TGG	0.404805	7.1:-81986019	MS.gene021905:CDS
ATTTAGAGAAATTAAACAAA+AGG	0.406967	7.1:-81986231	MS.gene021905:CDS
ATTCAAGGAATAGGAGTTGC+AGG	0.408252	7.1:-81985824	MS.gene021905:CDS
AAGGGTGTGATTGTGAATTT+GGG	0.425297	7.1:+81986893	None:intergenic
GGTGAAGGAATTCAAGGAAT+AGG	0.425888	7.1:-81985833	MS.gene021905:CDS
ACAAAAGGGTGTTGTCCAAA+TGG	0.444540	7.1:-81986216	MS.gene021905:CDS
GAGTCTGTTCATAAATAATT+GGG	0.449772	7.1:-81986371	MS.gene021905:CDS
GAGATTACTTCAATTGATAG+AGG	0.456941	7.1:-81985743	MS.gene021905:CDS
CAAAACTTCCTCCCTTTAGC+TGG	0.462356	7.1:-81986814	MS.gene021905:CDS
TTTAGAGAAATTAAACAAAA+GGG	0.466166	7.1:-81986230	MS.gene021905:CDS
TTTGGATTGAATTTGATGAA+TGG	0.473082	7.1:+81986760	None:intergenic
AACAAGTCAAAGAAAGTGAG+AGG	0.479248	7.1:+81986961	None:intergenic
TTTGGAGAGGGGAGTAGTAA+AGG	0.482127	7.1:-81986410	MS.gene021905:CDS
ACACACCATGCTGTTCTTGA+CGG	0.482364	7.1:-81986535	MS.gene021905:CDS
GAGAAACTTTGCATTGTTCG+TGG	0.482505	7.1:+81987010	None:intergenic
CAACCATGTTCATCAAAGCT+TGG	0.484613	7.1:-81986504	MS.gene021905:CDS
TATCGCCTTTGCTATACAAA+CGG	0.491635	7.1:+81986117	None:intergenic
CAAACGATGTTACCAGCTAA+AGG	0.503485	7.1:+81986802	None:intergenic
ATGATTGGTGAAGGAATTCA+AGG	0.503765	7.1:-81985839	MS.gene021905:CDS
AGTTGCAGGGTCAACAAGGT+TGG	0.506239	7.1:-81985810	MS.gene021905:CDS
TTTCGGAAGGCCTTCAAAAG+TGG	0.513160	7.1:-81985765	MS.gene021905:CDS
AAACGATGTTACCAGCTAAA+GGG	0.515290	7.1:+81986803	None:intergenic
ACAGAAAGTAAGGATTTGAA+AGG	0.528561	7.1:-81985704	MS.gene021905:CDS
GTTGCAGGGTCAACAAGGTT+GGG	0.534201	7.1:-81985809	MS.gene021905:CDS
TGGATAAGGGTGTTCTTGAT+GGG	0.534245	7.1:-81985892	MS.gene021905:CDS
ATTACATTGTGTTAAGAGCA+AGG	0.536608	7.1:+81986723	None:intergenic
TGTTCTTGAGAAGCAAGTGA+TGG	0.541641	7.1:-81985663	MS.gene021905:CDS
TGTTTCCGTTTGTATAGCAA+AGG	0.550669	7.1:-81986122	MS.gene021905:CDS
GAAAGTAAGGATTTGAAAGG+TGG	0.551682	7.1:-81985701	MS.gene021905:CDS
AATCTTGATCTGCAATCCAC+AGG	0.565697	7.1:+81986049	None:intergenic
GTGCTCCAAAACTTTATCGA+AGG	0.566459	7.1:+81986687	None:intergenic
TATTTGGCTATGATTGGTGA+AGG	0.577286	7.1:-81985848	MS.gene021905:CDS
TAGAAGTAGATACGTTCACC+TGG	0.583143	7.1:+81986922	None:intergenic
GAATCTGTTGATTCTAAGTG+AGG	0.596592	7.1:+81986625	None:intergenic
AATCTTTAAGCTTCTCACGT+TGG	0.603971	7.1:+81986321	None:intergenic
AGTTAAGGATTCTGTTCGAG+TGG	0.604204	7.1:-81986275	MS.gene021905:CDS
GTGTGATTGTGAATTTGGGA+GGG	0.604440	7.1:+81986897	None:intergenic
AAATTCAATCCAAATGATGA+CGG	0.606900	7.1:-81986751	MS.gene021905:CDS
AGACCAAGCTTTGATGAACA+TGG	0.608686	7.1:+81986501	None:intergenic
GGTGTGATTGTGAATTTGGG+AGG	0.618307	7.1:+81986896	None:intergenic
ACAAGTCAAAGAAAGTGAGA+GGG	0.619012	7.1:+81986962	None:intergenic
TAGGAGTTGCAGGGTCAACA+AGG	0.623066	7.1:-81985814	MS.gene021905:CDS
CGAGACAAAGAAGCTTCTTG+TGG	0.646679	7.1:+81986658	None:intergenic
ATGGTGTGTGCTTATACCAA+TGG	0.657084	7.1:+81986549	None:intergenic
TTCAAGGAATAGGAGTTGCA+GGG	0.658447	7.1:-81985823	MS.gene021905:CDS
CGATGTTACCAGCTAAAGGG+AGG	0.666686	7.1:+81986806	None:intergenic
GTGGTATTCAAGCTCACACG+CGG	0.700184	7.1:-81986256	MS.gene021905:CDS
AGAAGTAGATACGTTCACCT+GGG	0.704297	7.1:+81986923	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTGTTGCTAATAAAATTTA+TGG	-	chr7.1:81986729-81986748	MS.gene021905:CDS	15.0%
!!	ATTTAGAGAAATTAAACAAA+AGG	-	chr7.1:81986427-81986446	MS.gene021905:CDS	15.0%
!!	TTTAGAGAAATTAAACAAAA+GGG	-	chr7.1:81986428-81986447	MS.gene021905:CDS	15.0%
!!!	ATAACAATTTTTCCAAAACT+TGG	-	chr7.1:81986081-81986100	MS.gene021905:CDS	20.0%
!!!	TATGAAATTGATTTTGGTTT+CGG	-	chr7.1:81986876-81986895	MS.gene021905:CDS	20.0%
!	AAAACAAAAACGATGGATAA+GGG	-	chr7.1:81986753-81986772	MS.gene021905:CDS	25.0%
!	AAATTCAATCCAAATGATGA+CGG	-	chr7.1:81985907-81985926	MS.gene021905:CDS	25.0%
!	AACAGACTCTCAAAATTATT+TGG	+	chr7.1:81986276-81986295	None:intergenic	25.0%
!	ACAAAAGTTGACAATTTCTT+AGG	+	chr7.1:81986501-81986520	None:intergenic	25.0%
!	AGAGTCTGTTCATAAATAAT+TGG	-	chr7.1:81986286-81986305	MS.gene021905:CDS	25.0%
!	ATTGATAGAGGATTAACTAT+TGG	-	chr7.1:81986927-81986946	MS.gene021905:CDS	25.0%
!	GAGTCTGTTCATAAATAATT+GGG	-	chr7.1:81986287-81986306	MS.gene021905:CDS	25.0%
!	TCTAAAAAAGGATCTAAGTT+TGG	+	chr7.1:81986222-81986241	None:intergenic	25.0%
!	TCTAAATATTTGGCTATGAT+TGG	-	chr7.1:81986804-81986823	MS.gene021905:CDS	25.0%
!	TTTGGATTGAATTTGATGAA+TGG	+	chr7.1:81985901-81985920	None:intergenic	25.0%
!!	AGGTTTTCAAAAAAGAAAGA+AGG	+	chr7.1:81985787-81985806	None:intergenic	25.0%
!!	GGTTTTCAAAAAAGAAAGAA+GGG	+	chr7.1:81985786-81985805	None:intergenic	25.0%
!!	TTGAATTTGATGAATGGTTT+TGG	+	chr7.1:81985895-81985914	None:intergenic	25.0%
!!!	ATTTTCTTTTAGTTTTCCTG+TGG	-	chr7.1:81986593-81986612	MS.gene021905:CDS	25.0%
!!!	GGAGTATATGAAATTGATTT+TGG	-	chr7.1:81986870-81986889	MS.gene021905:CDS	25.0%
!!!	TATTGGTTTAACAGAAAGTA+AGG	-	chr7.1:81986944-81986963	MS.gene021905:CDS	25.0%
!!!	TTTCTGTTTTTTATCGCTTT+CGG	+	chr7.1:81986574-81986593	None:intergenic	25.0%
	AAAAAAGGATCTAAGTTTGG+TGG	+	chr7.1:81986219-81986238	None:intergenic	30.0%
	ACAGAAAGTAAGGATTTGAA+AGG	-	chr7.1:81986954-81986973	MS.gene021905:CDS	30.0%
	ATTACATTGTGTTAAGAGCA+AGG	+	chr7.1:81985938-81985957	None:intergenic	30.0%
	CAAAACAAAAACGATGGATA+AGG	-	chr7.1:81986752-81986771	MS.gene021905:CDS	30.0%
	CTCATTTGAACCAAAAGTTA+AGG	-	chr7.1:81986368-81986387	MS.gene021905:CDS	30.0%
	GACATTGACGTCTAAATATT+TGG	-	chr7.1:81986794-81986813	MS.gene021905:CDS	30.0%
	GAGATTACTTCAATTGATAG+AGG	-	chr7.1:81986915-81986934	MS.gene021905:CDS	30.0%
	TCCAAATGGTACATTGTAAA+AGG	-	chr7.1:81986456-81986475	MS.gene021905:CDS	30.0%
	TGGACTTATGAAAATGTGAT+AGG	+	chr7.1:81986320-81986339	None:intergenic	30.0%
	TTATGGCAAAACAAAAACGA+TGG	-	chr7.1:81986746-81986765	MS.gene021905:CDS	30.0%
!	CAATTTCCAAAGTAGTTTTC+AGG	+	chr7.1:81986648-81986667	None:intergenic	30.0%
!	GATGATTTTACAAAGGAAGA+TGG	-	chr7.1:81986699-81986718	MS.gene021905:CDS	30.0%
!	TCCTTTTACAATGTACCATT+TGG	+	chr7.1:81986460-81986479	None:intergenic	30.0%
!	TGAGTCAGATGATTTTACAA+AGG	-	chr7.1:81986692-81986711	MS.gene021905:CDS	30.0%
!	TGTTTCCTTCGATAAAGTTT+TGG	-	chr7.1:81985966-81985985	MS.gene021905:CDS	30.0%
!	TTCAAGCAATTTTTCGTGAA+GGG	-	chr7.1:81987024-81987043	MS.gene021905:CDS	30.0%
!	TTTCAAGCAATTTTTCGTGA+AGG	-	chr7.1:81987023-81987042	MS.gene021905:CDS	30.0%
!	TTTTACAAAGGAAGATGGTT+TGG	-	chr7.1:81986704-81986723	MS.gene021905:CDS	30.0%
!!	GATAATTGGTTTGCAGAATT+AGG	+	chr7.1:81985673-81985692	None:intergenic	30.0%
!!	TCCAAAAAGGGTTCTAAAAA+AGG	+	chr7.1:81986234-81986253	None:intergenic	30.0%
!!!	AAATTGATTTTGGTTTCGGA+AGG	-	chr7.1:81986880-81986899	MS.gene021905:CDS	30.0%
!!!	AAGATTTGAGTTTTGGAACA+AGG	+	chr7.1:81985807-81985826	None:intergenic	30.0%
!!!	TCACTTTCTTTGACTTGTTT+TGG	-	chr7.1:81985701-81985720	MS.gene021905:CDS	30.0%
!!!	TCCTTTTTTAGAACCCTTTT+TGG	-	chr7.1:81986230-81986249	MS.gene021905:CDS	30.0%
	AAACGATGTTACCAGCTAAA+GGG	+	chr7.1:81985858-81985877	None:intergenic	35.0%
	AACAAGTCAAAGAAAGTGAG+AGG	+	chr7.1:81985700-81985719	None:intergenic	35.0%
	AATCTTTAAGCTTCTCACGT+TGG	+	chr7.1:81986340-81986359	None:intergenic	35.0%
	ACAAGTCAAAGAAAGTGAGA+GGG	+	chr7.1:81985699-81985718	None:intergenic	35.0%
	ATGATTGGTGAAGGAATTCA+AGG	-	chr7.1:81986819-81986838	MS.gene021905:CDS	35.0%
	GAAAGTAAGGATTTGAAAGG+TGG	-	chr7.1:81986957-81986976	MS.gene021905:CDS	35.0%
	TATTTGGCTATGATTGGTGA+AGG	-	chr7.1:81986810-81986829	MS.gene021905:CDS	35.0%
	TGTTTCCGTTTGTATAGCAA+AGG	-	chr7.1:81986536-81986555	MS.gene021905:CDS	35.0%
!	AAGGGTGTGATTGTGAATTT+GGG	+	chr7.1:81985768-81985787	None:intergenic	35.0%
!	CCAATGGAAAAACCAAGTTT+TGG	+	chr7.1:81986096-81986115	None:intergenic	35.0%
!	CGAACAGAATCCTTAACTTT+TGG	+	chr7.1:81986381-81986400	None:intergenic	35.0%
!	TATCGCCTTTGCTATACAAA+CGG	+	chr7.1:81986544-81986563	None:intergenic	35.0%
!	TTGAAAGGTGGTGTTGAATT+TGG	-	chr7.1:81986969-81986988	MS.gene021905:CDS	35.0%
!!	ATTGTTCGTGGATTTTGATG+TGG	+	chr7.1:81985639-81985658	None:intergenic	35.0%
!!	CCAAAACTTGGTTTTTCCAT+TGG	-	chr7.1:81986093-81986112	MS.gene021905:CDS	35.0%
!!	GAAGTAATCTCCACTTTTGA+AGG	+	chr7.1:81986906-81986925	None:intergenic	35.0%
!!	GAATCTGTTGATTCTAAGTG+AGG	+	chr7.1:81986036-81986055	None:intergenic	35.0%
!!	GAGAGTGAAGATTTGAGTTT+TGG	+	chr7.1:81985814-81985833	None:intergenic	35.0%
!!	TGGATTTTGATGTGGTTGTT+TGG	+	chr7.1:81985631-81985650	None:intergenic	35.0%
!!	TTTAGAACCCTTTTTGGAGA+GGG	-	chr7.1:81986236-81986255	MS.gene021905:CDS	35.0%
!!!	AATGGTTTTGGAGAATCAGA+AGG	+	chr7.1:81985883-81985902	None:intergenic	35.0%
!!!	TCTTTGACTTGTTTTGGCTA+AGG	-	chr7.1:81985707-81985726	MS.gene021905:CDS	35.0%
!!!	TTTTAGAACCCTTTTTGGAG+AGG	-	chr7.1:81986235-81986254	MS.gene021905:CDS	35.0%
	AAGGAAACTCCGTCATCATT+TGG	+	chr7.1:81985919-81985938	None:intergenic	40.0%
	AAGTGAGAGGGAGAGATAAT+TGG	+	chr7.1:81985687-81985706	None:intergenic	40.0%
	AATCTTGATCTGCAATCCAC+AGG	+	chr7.1:81986612-81986631	None:intergenic	40.0%
	AGAAGTAGATACGTTCACCT+GGG	+	chr7.1:81985738-81985757	None:intergenic	40.0%
	AGTTAAGGATTCTGTTCGAG+TGG	-	chr7.1:81986383-81986402	MS.gene021905:CDS	40.0%
	ATGGTGTGTGCTTATACCAA+TGG	+	chr7.1:81986112-81986131	None:intergenic	40.0%
	ATTCAAGGAATAGGAGTTGC+AGG	-	chr7.1:81986834-81986853	MS.gene021905:CDS	40.0%
	CAAACGATGTTACCAGCTAA+AGG	+	chr7.1:81985859-81985878	None:intergenic	40.0%
	CAACCATGTTCATCAAAGCT+TGG	-	chr7.1:81986154-81986173	MS.gene021905:CDS	40.0%
	CCAGTTCCTGAAAACTACTT+TGG	-	chr7.1:81986639-81986658	MS.gene021905:CDS	40.0%
	CTTTAGCTGGTAACATCGTT+TGG	-	chr7.1:81985857-81985876	MS.gene021905:CDS	40.0%
	GAGAAACTTTGCATTGTTCG+TGG	+	chr7.1:81985651-81985670	None:intergenic	40.0%
	GGTGAAGGAATTCAAGGAAT+AGG	-	chr7.1:81986825-81986844	MS.gene021905:CDS	40.0%
	GTGCTCCAAAACTTTATCGA+AGG	+	chr7.1:81985974-81985993	None:intergenic	40.0%
	GTGTGATTGTGAATTTGGGA+GGG	+	chr7.1:81985764-81985783	None:intergenic	40.0%
	TAGAAGTAGATACGTTCACC+TGG	+	chr7.1:81985739-81985758	None:intergenic	40.0%
	TGTTCTTGAGAAGCAAGTGA+TGG	-	chr7.1:81986995-81987014	MS.gene021905:CDS	40.0%
	TTCAAGGAATAGGAGTTGCA+GGG	-	chr7.1:81986835-81986854	MS.gene021905:CDS	40.0%
!	AGACCAAGCTTTGATGAACA+TGG	+	chr7.1:81986160-81986179	None:intergenic	40.0%
!	ATTTTCCGTCAAGAACAGCA+TGG	+	chr7.1:81986131-81986150	None:intergenic	40.0%
!	GAAGGGTGTGATTGTGAATT+TGG	+	chr7.1:81985769-81985788	None:intergenic	40.0%
!!	AAGTAGTTTTCAGGAACTGG+TGG	+	chr7.1:81986639-81986658	None:intergenic	40.0%
!!	ACAAAAGGGTGTTGTCCAAA+TGG	-	chr7.1:81986442-81986461	MS.gene021905:CDS	40.0%
!!	ATGGATAAGGGTGTTCTTGA+TGG	-	chr7.1:81986765-81986784	MS.gene021905:CDS	40.0%
!!	CCAAAGTAGTTTTCAGGAAC+TGG	+	chr7.1:81986642-81986661	None:intergenic	40.0%
!!	TGGATAAGGGTGTTCTTGAT+GGG	-	chr7.1:81986766-81986785	MS.gene021905:CDS	40.0%
!!	TTAGAACCCTTTTTGGAGAG+GGG	-	chr7.1:81986237-81986256	MS.gene021905:CDS	40.0%
!!!	TAAAGGGAGGAAGTTTTGGA+GGG	+	chr7.1:81985842-81985861	None:intergenic	40.0%
	ACACACCATGCTGTTCTTGA+CGG	-	chr7.1:81986123-81986142	MS.gene021905:CDS	45.0%
	ACTACTCCCCTCTCCAAAAA+GGG	+	chr7.1:81986246-81986265	None:intergenic	45.0%
	CAAAACTTCCTCCCTTTAGC+TGG	-	chr7.1:81985844-81985863	MS.gene021905:CDS	45.0%
	CGAGACAAAGAAGCTTCTTG+TGG	+	chr7.1:81986003-81986022	None:intergenic	45.0%
	GGTGTGATTGTGAATTTGGG+AGG	+	chr7.1:81985765-81985784	None:intergenic	45.0%
	TACTACTCCCCTCTCCAAAA+AGG	+	chr7.1:81986247-81986266	None:intergenic	45.0%
	TTTCGGAAGGCCTTCAAAAG+TGG	-	chr7.1:81986893-81986912	MS.gene021905:CDS	45.0%
	TTTGGAGAGGGGAGTAGTAA+AGG	-	chr7.1:81986248-81986267	MS.gene021905:CDS	45.0%
!	CAGCTAAAGGGAGGAAGTTT+TGG	+	chr7.1:81985846-81985865	None:intergenic	45.0%
!!	CTAAAGGGAGGAAGTTTTGG+AGG	+	chr7.1:81985843-81985862	None:intergenic	45.0%
!!	TTTTGGCTAAGGTTTCACCC+AGG	-	chr7.1:81985718-81985737	MS.gene021905:CDS	45.0%
	AGTTGCAGGGTCAACAAGGT+TGG	-	chr7.1:81986848-81986867	MS.gene021905:CDS	50.0%
	CGATGTTACCAGCTAAAGGG+AGG	+	chr7.1:81985855-81985874	None:intergenic	50.0%
	GTGGTATTCAAGCTCACACG+CGG	-	chr7.1:81986402-81986421	MS.gene021905:CDS	50.0%
	GTTGCAGGGTCAACAAGGTT+GGG	-	chr7.1:81986849-81986868	MS.gene021905:CDS	50.0%
	TAGGAGTTGCAGGGTCAACA+AGG	-	chr7.1:81986844-81986863	MS.gene021905:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.1	gene	81985619	81987061	81985619	ID=MS.gene021905
chr7.1	mRNA	81985619	81987061	81985619	ID=MS.gene021905.t1;Parent=MS.gene021905
chr7.1	exon	81985619	81987061	81985619	ID=MS.gene021905.t1.exon1;Parent=MS.gene021905.t1
chr7.1	CDS	81985619	81987061	81985619	ID=cds.MS.gene021905.t1;Parent=MS.gene021905.t1

Gene Sequence

>MS.gene021905

ATGGCGTCTCCAAACAACCACATCAAAATCCACGAACAATGCAAAGTTTCTCCTAATTCTGCAAACCAATTATCTCTCCCTCTCACTTTCTTTGACTTGTTTTGGCTAAGGTTTCACCCAGGTGAACGTATCTACTTCTACACCCTCCCAAATTCACAATCACACCCTTCTTTCTTTTTTGAAAACCTTGTTCCAAAACTCAAATCTTCACTCTCTTTAACCCTCCAAAACTTCCTCCCTTTAGCTGGTAACATCGTTTGGCCTTCTGATTCTCCAAAACCATTCATCAAATTCAATCCAAATGATGACGGAGTTTCCTTGCTCTTAACACAATGTAATGACGATGATGTTTCCTTCGATAAAGTTTTGGAGCACAATTCACCACAAGAAGCTTCTTTGTCTCGTTCGTTTGTACCTCACTTAGAATCAACAGATTCTTTTGCTTCTATTATTTCAATTCAGATAACAATTTTTCCAAAACTTGGTTTTTCCATTGGTATAAGCACACACCATGCTGTTCTTGACGGAAAATCTTCAACCATGTTCATCAAAGCTTGGTCTTCTATATCTAAATCTTTTCAAGAAGAAGAAATGAAACCACCAAACTTAGATCCTTTTTTAGAACCCTTTTTGGAGAGGGGAGTAGTAAAGGATCCAAATAATTTTGAGAGTCTGTTCATAAATAATTGGGACAAAATCCTATCACATTTTCATAAGTCCAACGTGAGAAGCTTAAAGATTATGTCAGACTCATTTGAACCAAAAGTTAAGGATTCTGTTCGAGTGGTATTCAAGCTCACACGCGGAGATTTAGAGAAATTAAACAAAAGGGTGTTGTCCAAATGGTACATTGTAAAAGGAGCACAAGAACATGAACAACCTAAGAAATTGTCAACTTTTGTTCTTACATGTGCTTATGTTTCCGTTTGTATAGCAAAGGCGATACAACAATCCGAAAGCGATAAAAAACAGAAATTTTCTTTTAGTTTTCCTGTGGATTGCAGATCAAGATTAGATCCACCAGTTCCTGAAAACTACTTTGGAAATTGTGTATGTAATCATATAGTTGATACTGAGTCAGATGATTTTACAAAGGAAGATGGTTTGGTAATTGTTGCTAATAAAATTTATGGCAAAACAAAAACGATGGATAAGGGTGTTCTTGATGGGATAGAGACATTGACGTCTAAATATTTGGCTATGATTGGTGAAGGAATTCAAGGAATAGGAGTTGCAGGGTCAACAAGGTTGGGAGTATATGAAATTGATTTTGGTTTCGGAAGGCCTTCAAAAGTGGAGATTACTTCAATTGATAGAGGATTAACTATTGGTTTAACAGAAAGTAAGGATTTGAAAGGTGGTGTTGAATTTGGACTTGTTCTTGAGAAGCAAGTGATGGATTTGTTTCAAGCAATTTTTCGTGAAGGGTTGTATTTTGATTGA

Protein sequence

>MS.gene021905.t1

MASPNNHIKIHEQCKVSPNSANQLSLPLTFFDLFWLRFHPGERIYFYTLPNSQSHPSFFFENLVPKLKSSLSLTLQNFLPLAGNIVWPSDSPKPFIKFNPNDDGVSLLLTQCNDDDVSFDKVLEHNSPQEASLSRSFVPHLESTDSFASIISIQITIFPKLGFSIGISTHHAVLDGKSSTMFIKAWSSISKSFQEEEMKPPNLDPFLEPFLERGVVKDPNNFESLFINNWDKILSHFHKSNVRSLKIMSDSFEPKVKDSVRVVFKLTRGDLEKLNKRVLSKWYIVKGAQEHEQPKKLSTFVLTCAYVSVCIAKAIQQSESDKKQKFSFSFPVDCRSRLDPPVPENYFGNCVCNHIVDTESDDFTKEDGLVIVANKIYGKTKTMDKGVLDGIETLTSKYLAMIGEGIQGIGVAGSTRLGVYEIDFGFGRPSKVEITSIDRGLTIGLTESKDLKGGVEFGLVLEKQVMDLFQAIFREGLYFD