AlfalfaGEDB | MS.gene91614

M. sativa cultivar XinJiangDaYe / MS.gene91614

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016740 GO:0016746 GO:0016747
PFAM: PF02458.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91614.t1	XP_013447578.1	80.4	484	90	4	1	480	1	483	1.10E-218	769.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91614.t1	Q9LJB4	37.8	484	258	12	1	477	1	448	5.6e-78	293.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91614.t1	B4Y0U1	80.4	484	90	4	1	480	1	483	8.0e-219	769.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene069423	MS.gene91614	PPI
MS.gene91614	MS.gene44647	PPI
MS.gene069422	MS.gene91614	PPI
MS.gene91614	MS.gene35740	PPI
MS.gene35744	MS.gene91614	PPI
MS.gene44643	MS.gene91614	PPI
MS.gene028999	MS.gene91614	PPI
MS.gene50379	MS.gene91614	PPI
MS.gene91614	MS.gene011799	PPI
MS.gene91614	MS.gene002544	PPI
MS.gene91614	MS.gene50420	PPI
MS.gene31545	MS.gene91614	PPI
MS.gene91614	MS.gene021905	PPI
MS.gene44633	MS.gene91614	PPI
MS.gene91614	MS.gene070270	PPI
MS.gene021915	MS.gene91614	PPI
MS.gene91614	MS.gene50382	PPI
MS.gene021904	MS.gene91614	PPI
MS.gene073299	MS.gene91614	PPI
MS.gene50382	MS.gene91614	PPI
MS.gene91597	MS.gene91614	PPI
MS.gene021916	MS.gene91614	PPI
MS.gene31546	MS.gene91614	PPI
MS.gene35741	MS.gene91614	PPI
MS.gene99219	MS.gene91614	PPI
MS.gene021914	MS.gene91614	PPI
MS.gene91614	MS.gene87191	PPI
MS.gene91614	MS.gene48584	PPI
MS.gene90496	MS.gene91614	PPI
MS.gene91598	MS.gene91614	PPI
MS.gene56795	MS.gene91614	PPI
MS.gene91614	MS.gene016477	PPI
MS.gene91614	MS.gene021913	PPI
MS.gene78818	MS.gene91614	PPI
MS.gene91614	MS.gene02573	PPI
MS.gene028996	MS.gene91614	PPI
MS.gene000763	MS.gene91614	PPI
MS.gene91614	MS.gene028994	PPI
MS.gene28251	MS.gene91614	PPI
MS.gene91613	MS.gene91614	PPI
MS.gene000764	MS.gene91614	PPI
MS.gene91615	MS.gene91614	PPI
MS.gene48582	MS.gene91614	PPI
MS.gene38977	MS.gene91614	PPI
MS.gene90491	MS.gene91614	PPI
MS.gene99223	MS.gene91614	PPI
MS.gene90492	MS.gene91614	PPI
MS.gene91612	MS.gene91614	PPI
MS.gene48585	MS.gene91614	PPI
MS.gene91614	MS.gene91613	PPI
MS.gene91614	MS.gene043350	PPI
MS.gene011837	MS.gene91614	PPI
MS.gene066634	MS.gene91614	PPI
MS.gene91614	MS.gene56798	PPI
MS.gene043346	MS.gene91614	PPI
MS.gene91599	MS.gene91614	PPI
MS.gene91614	MS.gene96337	PPI
MS.gene91614	MS.gene066658	PPI
MS.gene91614	MS.gene87496	PPI
MS.gene49853	MS.gene91614	PPI
MS.gene063865	MS.gene91614	PPI
MS.gene91614	MS.gene070019	PPI
MS.gene91614	MS.gene021917	PPI
MS.gene007831	MS.gene91614	PPI
MS.gene021910	MS.gene91614	PPI
MS.gene069767	MS.gene91614	PPI
MS.gene91614	MS.gene91596	PPI
MS.gene004218	MS.gene91614	PPI
MS.gene44644	MS.gene91614	PPI
MS.gene35745	MS.gene91614	PPI
MS.gene011796	MS.gene91614	PPI
MS.gene011801	MS.gene91614	PPI
MS.gene87537	MS.gene91614	PPI
MS.gene91614	MS.gene99218	PPI
MS.gene99222	MS.gene91614	PPI
MS.gene91614	MS.gene56799	PPI
MS.gene60208	MS.gene91614	PPI
MS.gene90493	MS.gene91614	PPI
MS.gene032349	MS.gene91614	PPI
MS.gene013787	MS.gene91614	PPI
MS.gene066656	MS.gene91614	PPI
MS.gene91614	MS.gene069767	PPI
MS.gene021906	MS.gene91614	PPI
MS.gene31542	MS.gene91614	PPI
MS.gene87532	MS.gene91614	PPI
MS.gene91614	MS.gene000763	PPI
MS.gene46265	MS.gene91614	PPI
MS.gene066660	MS.gene91614	PPI
MS.gene063868	MS.gene91614	PPI
MS.gene31540	MS.gene91614	PPI
MS.gene066658	MS.gene91614	PPI
MS.gene48581	MS.gene91614	PPI
MS.gene44640	MS.gene91614	PPI
MS.gene91614	MS.gene011716	PPI
MS.gene91614	MS.gene28251	PPI
MS.gene91614	MS.gene91595	PPI
MS.gene043348	MS.gene91614	PPI
MS.gene99221	MS.gene91614	PPI
MS.gene03202	MS.gene91614	PPI
MS.gene91614	MS.gene043352	PPI
MS.gene91614	MS.gene50384	PPI
MS.gene91614	MS.gene87577	PPI
MS.gene91614	MS.gene066701	PPI
MS.gene91614	MS.gene021909	PPI
MS.gene91614	MS.gene50355	PPI
MS.gene91614	MS.gene91612	PPI
MS.gene012360	MS.gene91614	PPI
MS.gene44642	MS.gene91614	PPI
MS.gene028998	MS.gene91614	PPI
MS.gene56796	MS.gene91614	PPI
MS.gene91609	MS.gene91614	PPI
MS.gene91614	MS.gene064101	PPI
MS.gene50355	MS.gene91614	PPI
MS.gene011798	MS.gene91614	PPI
MS.gene91611	MS.gene91614	PPI
MS.gene48584	MS.gene91614	PPI
MS.gene91614	MS.gene50356	PPI
MS.gene91614	MS.gene87536	PPI
MS.gene91614	MS.gene56797	PPI
MS.gene91614	MS.gene44630	PPI
MS.gene32261	MS.gene91614	PPI
MS.gene91614	MS.gene069422	PPI
MS.gene44463	MS.gene91614	PPI
MS.gene066657	MS.gene91614	PPI
MS.gene56798	MS.gene91614	PPI
MS.gene91614	MS.gene016532	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91614.t1	MTR_7g013850	80.165	484	91	4	1	480	1	483	0.0	773
MS.gene91614.t1	MTR_7g014200	59.091	484	175	12	1	477	1	468	0.0	538
MS.gene91614.t1	MTR_7g014160	58.246	479	183	11	5	477	6	473	0.0	538
MS.gene91614.t1	MTR_7g014610	58.799	483	185	9	1	477	1	475	0.0	520
MS.gene91614.t1	MTR_7g014620	52.711	461	182	7	19	477	6	432	4.34e-150	435
MS.gene91614.t1	MTR_7g014630	57.143	301	116	7	6	301	7	299	3.13e-110	331
MS.gene91614.t1	MTR_6g015315	39.149	470	262	10	9	477	6	452	5.21e-102	313
MS.gene91614.t1	MTR_7g014330	39.749	478	255	15	9	477	3	456	3.04e-100	308
MS.gene91614.t1	MTR_6g015830	39.794	485	243	14	9	477	9	460	7.10e-99	305
MS.gene91614.t1	MTR_6g015320	36.983	484	268	12	1	477	1	454	7.95e-99	305
MS.gene91614.t1	MTR_6g015850	39.104	491	251	16	1	477	1	457	1.55e-98	304
MS.gene91614.t1	MTR_7g014230	38.622	479	263	12	9	479	5	460	2.03e-98	304
MS.gene91614.t1	MTR_7g014250	38.994	477	260	14	9	477	3	456	5.21e-98	303
MS.gene91614.t1	MTR_6g015815	40.700	457	240	11	25	477	31	460	1.44e-96	299
MS.gene91614.t1	MTR_3g028210	35.464	485	270	16	8	477	8	464	4.80e-75	243
MS.gene91614.t1	MTR_7g014180	70.391	179	46	5	1	178	1	173	3.53e-74	231
MS.gene91614.t1	MTR_7g014670	55.959	193	83	2	289	480	71	262	4.32e-68	219
MS.gene91614.t1	MTR_2g089765	32.515	489	281	15	11	477	21	482	3.67e-67	223
MS.gene91614.t1	MTR_7g014360	34.152	407	218	12	78	477	23	386	1.44e-64	214
MS.gene91614.t1	MTR_2g089670	32.181	463	275	14	26	477	43	477	2.05e-61	208
MS.gene91614.t1	MTR_2g089650	28.916	498	298	15	1	477	1	463	1.33e-59	203
MS.gene91614.t1	MTR_2g089735	31.839	446	270	11	42	477	40	461	1.55e-59	202
MS.gene91614.t1	MTR_3g087640	29.779	497	287	11	9	477	10	472	6.40e-59	201
MS.gene91614.t1	MTR_7g015860	30.629	493	282	17	8	477	5	460	3.42e-55	191
MS.gene91614.t1	MTR_2g089755	28.970	466	288	13	28	477	37	475	4.35e-54	189
MS.gene91614.t1	MTR_8g012750	30.998	471	280	15	26	475	24	470	5.67e-53	186
MS.gene91614.t1	MTR_6g015310	30.617	405	208	12	51	455	2	333	2.38e-48	169
MS.gene91614.t1	MTR_7g014310	29.730	333	165	9	130	460	41	306	5.07e-39	144
MS.gene91614.t1	MTR_2g089665	25.164	457	201	14	11	454	7	335	2.61e-25	107
MS.gene91614.t1	MTR_3g435300	30.000	280	164	9	9	276	4	263	5.50e-25	104
MS.gene91614.t1	MTR_4g007540	24.017	483	289	21	8	470	3	427	2.15e-21	96.7
MS.gene91614.t1	MTR_8g075610	24.190	401	233	16	44	429	30	374	1.56e-20	94.0
MS.gene91614.t1	MTR_3g049150	24.374	439	268	18	63	478	51	448	2.21e-18	87.8
MS.gene91614.t1	MTR_7g086000	24.400	500	304	19	7	477	3	457	4.48e-18	87.0
MS.gene91614.t1	MTR_3g070170	24.724	453	272	18	8	433	12	422	6.25e-18	86.7
MS.gene91614.t1	MTR_2g064495	24.406	463	272	23	32	475	28	431	1.32e-17	85.5
MS.gene91614.t1	MTR_7g014210	38.318	107	64	2	335	439	24	130	1.58e-17	80.5
MS.gene91614.t1	MTR_2g105330	24.729	461	273	23	32	475	28	431	5.78e-17	83.2
MS.gene91614.t1	MTR_7g014205	37.615	109	56	2	332	439	17	114	1.01e-16	76.6
MS.gene91614.t1	MTR_8g096530	24.599	374	233	10	61	429	53	382	3.43e-16	81.3
MS.gene91614.t1	MTR_3g026950	23.448	435	270	17	64	473	51	447	4.65e-16	80.9
MS.gene91614.t1	MTR_3g088420	23.188	483	263	15	17	467	14	420	6.12e-16	80.1
MS.gene91614.t1	MTR_4g097090	23.270	477	289	19	26	475	28	454	1.08e-15	79.7
MS.gene91614.t1	MTR_4g022800	22.410	473	288	17	17	467	14	429	1.10e-15	79.3
MS.gene91614.t1	MTR_4g022720	23.158	475	275	18	17	467	14	422	2.65e-15	78.2
MS.gene91614.t1	MTR_4g127650	23.126	467	270	17	44	477	46	456	4.69e-15	77.8
MS.gene91614.t1	MTR_7g085930	22.403	491	329	15	6	477	4	461	7.81e-15	77.0
MS.gene91614.t1	MTR_7g085990	23.510	302	200	9	50	349	42	314	6.67e-14	73.9
MS.gene91614.t1	MTR_3g101970	24.208	442	261	20	8	433	6	389	1.83e-13	72.8
MS.gene91614.t1	MTR_7g014340	53.521	71	26	2	9	75	3	70	4.67e-13	65.5
MS.gene91614.t1	MTR_8g079970	22.899	476	276	18	17	470	15	421	1.71e-12	69.7
MS.gene91614.t1	MTR_4g022825	21.798	445	262	16	28	446	24	408	3.95e-12	68.6
MS.gene91614.t1	MTR_8g467580	25.641	429	245	21	17	429	15	385	5.18e-12	68.2
MS.gene91614.t1	MTR_8g079950	24.043	470	278	17	17	470	15	421	9.61e-12	67.4
MS.gene91614.t1	MTR_7g014220	56.604	53	20	1	25	77	15	64	6.08e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91614.t1	AT3G29590	38.017	484	257	12	1	477	1	448	3.14e-89	280
MS.gene91614.t1	AT3G29670	38.542	480	254	13	8	478	3	450	2.94e-87	275
MS.gene91614.t1	AT5G39080	38.689	473	262	15	1	467	1	451	3.91e-87	275
MS.gene91614.t1	AT5G39050	37.320	485	266	15	6	477	8	467	6.03e-86	272
MS.gene91614.t1	AT3G29635	37.422	481	261	14	8	477	3	454	9.88e-85	268
MS.gene91614.t1	AT5G39090	36.632	475	265	14	6	477	6	447	7.91e-83	263
MS.gene91614.t1	AT1G03940	31.515	495	274	12	9	477	9	464	1.48e-70	232
MS.gene91614.t1	AT5G61160	33.827	473	277	14	8	473	3	446	6.87e-69	227
MS.gene91614.t1	AT3G29680	34.664	476	267	14	6	473	4	443	5.50e-68	224
MS.gene91614.t1	AT1G03495	31.183	372	215	11	1	360	1	343	5.84e-45	160
MS.gene91614.t1	AT5G48930	24.431	483	297	20	8	475	3	432	4.86e-20	92.8
MS.gene91614.t1	AT3G29720	33.333	189	103	8	99	278	10	184	2.64e-16	78.2
MS.gene91614.t1	AT3G50280	22.511	462	287	16	19	468	18	420	4.25e-15	77.8
MS.gene91614.t1	AT5G57840	23.142	471	291	16	25	477	21	438	1.28e-14	76.3
MS.gene91614.t1	AT3G29690	29.801	151	100	2	328	477	33	178	1.49e-14	72.4
MS.gene91614.t1	AT2G39980	23.503	451	308	14	1	433	1	432	1.21e-13	73.6
MS.gene91614.t1	AT5G07850	23.714	447	285	16	1	433	1	405	1.10e-12	70.5
MS.gene91614.t1	AT1G28680	21.138	369	247	9	67	432	62	389	1.70e-12	69.7
MS.gene91614.t1	AT5G23970	22.614	482	265	20	16	470	11	411	3.70e-12	68.6
MS.gene91614.t1	AT5G41040	23.908	435	250	22	61	473	63	438	1.24e-11	67.0
MS.gene91614.t1	AT5G41040	23.908	435	250	22	61	473	79	454	1.87e-11	66.6
MS.gene91614.t1	AT4G31910	22.714	339	226	7	60	389	57	368	2.13e-11	66.2
MS.gene91614.t1	AT3G48720	21.583	417	270	14	67	474	61	429	4.69e-11	65.1

Find 74 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAGACTCTCAAAATTATT+TGG	0.163672	7.4:+85183895	None:intergenic
GAAGGGTGTGATTGTGAATT+TGG	0.182607	7.4:+85184402	None:intergenic
AGAGTCTGTTCATAAATAAT+TGG	0.191926	7.4:-85183882	MS.gene91614:CDS
GACATTGACGTCTAAATATT+TGG	0.199250	7.4:-85183374	MS.gene91614:CDS
TCACTTTCTTTGACTTGTTT+TGG	0.206968	7.4:-85184467	MS.gene91614:CDS
TTGAAAGGTGGTGTTGAATT+TGG	0.214046	7.4:-85183199	MS.gene91614:CDS
GAGAGTGAAGATTTGAGTTT+TGG	0.216722	7.4:+85184357	None:intergenic
GATAATTGGTTTGCAGAATT+AGG	0.221219	7.4:+85184498	None:intergenic
CAGCTAAAGGGAGGAAGTTT+TGG	0.225428	7.4:+85184325	None:intergenic
ACAAAAGTTGACAATTTCTT+AGG	0.235091	7.4:+85183670	None:intergenic
TACTACTCCCCTCTCCAAAA+AGG	0.248355	7.4:+85183924	None:intergenic
ATTGTTGCTAATAAAATTTA+TGG	0.257747	7.4:-85183439	MS.gene91614:CDS
GGAGTATATGAAATTGATTT+TGG	0.266147	7.4:-85183298	MS.gene91614:CDS
TTGAATTTGATGAATGGTTT+TGG	0.270000	7.4:+85184276	None:intergenic
CGAACGGAATCCTTAACTTT+TGG	0.284718	7.4:+85183790	None:intergenic
AAGTGAGAGGGAGAGATAAT+TGG	0.293242	7.4:+85184484	None:intergenic
TCCAAATGGTACATTGTAAA+AGG	0.320135	7.4:-85183712	MS.gene91614:CDS
TGTTTCCTTCGATAAAGTTT+TGG	0.341922	7.4:-85184202	MS.gene91614:CDS
CTTTAGCTGGTAACATCGTT+TGG	0.362068	7.4:-85184311	MS.gene91614:CDS
TCTAAATATTTGGCTATGAT+TGG	0.368222	7.4:-85183364	MS.gene91614:CDS
ATTGATAGAGGATTAACTAT+TGG	0.368461	7.4:-85183241	MS.gene91614:CDS
CTCATTTGAACCAAAAGTTA+AGG	0.369807	7.4:-85183800	MS.gene91614:CDS
TGGACTTATGAAAATGTGAT+AGG	0.386400	7.4:+85183851	None:intergenic
AAGGAAACTCCGTCATCATT+TGG	0.395044	7.4:+85184252	None:intergenic
ATGGATAAGGGTGTTCTTGA+TGG	0.402404	7.4:-85183403	MS.gene91614:CDS
CCAGTTCCTGAAAACTACTT+TGG	0.404805	7.4:-85183529	MS.gene91614:CDS
ATTTAGAGAAATTAAACAAA+AGG	0.406967	7.4:-85183741	MS.gene91614:CDS
ATTCAAGGAATAGGAGTTGC+AGG	0.408252	7.4:-85183334	MS.gene91614:CDS
AAGGGTGTGATTGTGAATTT+GGG	0.425297	7.4:+85184403	None:intergenic
GGTGAAGGAATTCAAGGAAT+AGG	0.425888	7.4:-85183343	MS.gene91614:CDS
ACAAAAGGGTGTTGTCCAAA+TGG	0.444540	7.4:-85183726	MS.gene91614:CDS
GAGTCTGTTCATAAATAATT+GGG	0.449772	7.4:-85183881	MS.gene91614:CDS
GAGATTACTTCAATTGATAG+AGG	0.456941	7.4:-85183253	MS.gene91614:CDS
CAAAACTTCCTCCCTTTAGC+TGG	0.462356	7.4:-85184324	MS.gene91614:CDS
TTTAGAGAAATTAAACAAAA+GGG	0.466166	7.4:-85183740	MS.gene91614:CDS
TTTGGATTGAATTTGATGAA+TGG	0.473082	7.4:+85184270	None:intergenic
AACAAGTCAAAGAAAGTGAG+AGG	0.479248	7.4:+85184471	None:intergenic
TTTGGAGAGGGGAGTAGTAA+AGG	0.482127	7.4:-85183920	MS.gene91614:CDS
ACACACCATGCTGTTCTTGA+CGG	0.482364	7.4:-85184045	MS.gene91614:CDS
GAGAAACTTTGCATTGTTCG+TGG	0.482505	7.4:+85184520	None:intergenic
CAACCATGTTCATCAAAGCT+TGG	0.484613	7.4:-85184014	MS.gene91614:CDS
TATCGCCTTTGCTATACAAA+CGG	0.491635	7.4:+85183627	None:intergenic
CAAACGATGTTACCAGCTAA+AGG	0.503485	7.4:+85184312	None:intergenic
ATGATTGGTGAAGGAATTCA+AGG	0.503765	7.4:-85183349	MS.gene91614:CDS
AGTTGCAGGGTCAACAAGGT+TGG	0.506239	7.4:-85183320	MS.gene91614:CDS
TTTCGGAAGGCCTTCAAAAG+TGG	0.513160	7.4:-85183275	MS.gene91614:CDS
AAACGATGTTACCAGCTAAA+GGG	0.515290	7.4:+85184313	None:intergenic
ACAGAAAGTAATGATTTGAA+AGG	0.524129	7.4:-85183214	MS.gene91614:CDS
GTTGCAGGGTCAACAAGGTT+GGG	0.534201	7.4:-85183319	MS.gene91614:CDS
TGGATAAGGGTGTTCTTGAT+GGG	0.534245	7.4:-85183402	MS.gene91614:CDS
ATTACATTGTGTTAAGAGCA+AGG	0.536608	7.4:+85184233	None:intergenic
GAAAGTAATGATTTGAAAGG+TGG	0.539031	7.4:-85183211	MS.gene91614:CDS
TGTTCTTGAGAAGCAAGTGA+TGG	0.541641	7.4:-85183173	MS.gene91614:CDS
TGTTTCCGTTTGTATAGCAA+AGG	0.550669	7.4:-85183632	MS.gene91614:CDS
AATCTTGATCTGCAATCCAC+AGG	0.565697	7.4:+85183559	None:intergenic
GTGCTCCAAAACTTTATCGA+AGG	0.566459	7.4:+85184197	None:intergenic
TATTTGGCTATGATTGGTGA+AGG	0.577286	7.4:-85183358	MS.gene91614:CDS
TAGAAGTAGATACGTTCACC+TGG	0.583143	7.4:+85184432	None:intergenic
GAATCTGTTGATTCTAAGTG+AGG	0.596592	7.4:+85184135	None:intergenic
AATCTTTAAGCTTCTCACGT+TGG	0.603971	7.4:+85183831	None:intergenic
GTGTGATTGTGAATTTGGGA+GGG	0.604440	7.4:+85184407	None:intergenic
AAATTCAATCCAAATGATGA+CGG	0.606900	7.4:-85184261	MS.gene91614:CDS
AGACCAAGCTTTGATGAACA+TGG	0.608686	7.4:+85184011	None:intergenic
AGTTAAGGATTCCGTTCGAG+TGG	0.609297	7.4:-85183785	MS.gene91614:CDS
GGTGTGATTGTGAATTTGGG+AGG	0.618307	7.4:+85184406	None:intergenic
ACAAGTCAAAGAAAGTGAGA+GGG	0.619012	7.4:+85184472	None:intergenic
TAGGAGTTGCAGGGTCAACA+AGG	0.623066	7.4:-85183324	MS.gene91614:CDS
CGAGACAAAGAAGCTTCTTG+TGG	0.646679	7.4:+85184168	None:intergenic
ATGGTGTGTGCTTATACCAA+TGG	0.657084	7.4:+85184059	None:intergenic
TTCAAGGAATAGGAGTTGCA+GGG	0.658447	7.4:-85183333	MS.gene91614:CDS
CGATGTTACCAGCTAAAGGG+AGG	0.666686	7.4:+85184316	None:intergenic
GAGCGTGAATACCACTCGAA+CGG	0.670435	7.4:+85183774	None:intergenic
GTGGTATTCACGCTCACACG+CGG	0.681785	7.4:-85183766	MS.gene91614:CDS
AGAAGTAGATACGTTCACCT+GGG	0.704297	7.4:+85184433	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTGTTGCTAATAAAATTTA+TGG	-	chr7.4:85184239-85184258	MS.gene91614:CDS	15.0%
!!	ATTTAGAGAAATTAAACAAA+AGG	-	chr7.4:85183937-85183956	MS.gene91614:CDS	15.0%
!!	TTTAGAGAAATTAAACAAAA+GGG	-	chr7.4:85183938-85183957	MS.gene91614:CDS	15.0%
!!!	TATGAAATTGATTTTGGTTT+CGG	-	chr7.4:85184386-85184405	MS.gene91614:CDS	20.0%
!	AAAACAAAAACGATGGATAA+GGG	-	chr7.4:85184263-85184282	MS.gene91614:CDS	25.0%
!	AAATTCAATCCAAATGATGA+CGG	-	chr7.4:85183417-85183436	MS.gene91614:CDS	25.0%
!	AACAGACTCTCAAAATTATT+TGG	+	chr7.4:85183786-85183805	None:intergenic	25.0%
!	ACAAAAGTTGACAATTTCTT+AGG	+	chr7.4:85184011-85184030	None:intergenic	25.0%
!	ACAGAAAGTAATGATTTGAA+AGG	-	chr7.4:85184464-85184483	MS.gene91614:CDS	25.0%
!	AGAGTCTGTTCATAAATAAT+TGG	-	chr7.4:85183796-85183815	MS.gene91614:CDS	25.0%
!	ATTGATAGAGGATTAACTAT+TGG	-	chr7.4:85184437-85184456	MS.gene91614:CDS	25.0%
!	GAGTCTGTTCATAAATAATT+GGG	-	chr7.4:85183797-85183816	MS.gene91614:CDS	25.0%
!	TCTAAAAAAGGATCTAAGTT+TGG	+	chr7.4:85183732-85183751	None:intergenic	25.0%
!	TCTAAATATTTGGCTATGAT+TGG	-	chr7.4:85184314-85184333	MS.gene91614:CDS	25.0%
!	TTTGGATTGAATTTGATGAA+TGG	+	chr7.4:85183411-85183430	None:intergenic	25.0%
!!	AGGTTTTCAAAAAAGAAAGA+AGG	+	chr7.4:85183297-85183316	None:intergenic	25.0%
!!	GGTTTTCAAAAAAGAAAGAA+GGG	+	chr7.4:85183296-85183315	None:intergenic	25.0%
!!	TTGAATTTGATGAATGGTTT+TGG	+	chr7.4:85183405-85183424	None:intergenic	25.0%
!!!	AAAAACCAAGTTTTGGAAAA+AGG	+	chr7.4:85183599-85183618	None:intergenic	25.0%
!!!	AAAACCAAGTTTTGGAAAAA+GGG	+	chr7.4:85183598-85183617	None:intergenic	25.0%
!!!	ATTTTCTTTTAGTTTTCCTG+TGG	-	chr7.4:85184103-85184122	MS.gene91614:CDS	25.0%
!!!	GGAGTATATGAAATTGATTT+TGG	-	chr7.4:85184380-85184399	MS.gene91614:CDS	25.0%
!!!	TTTCTGTTTTTTATCGCTTT+CGG	+	chr7.4:85184084-85184103	None:intergenic	25.0%
	AAAAAAGGATCTAAGTTTGG+TGG	+	chr7.4:85183729-85183748	None:intergenic	30.0%
	ATTACATTGTGTTAAGAGCA+AGG	+	chr7.4:85183448-85183467	None:intergenic	30.0%
	CAAAACAAAAACGATGGATA+AGG	-	chr7.4:85184262-85184281	MS.gene91614:CDS	30.0%
	CTCATTTGAACCAAAAGTTA+AGG	-	chr7.4:85183878-85183897	MS.gene91614:CDS	30.0%
	GAAAGTAATGATTTGAAAGG+TGG	-	chr7.4:85184467-85184486	MS.gene91614:CDS	30.0%
	GACATTGACGTCTAAATATT+TGG	-	chr7.4:85184304-85184323	MS.gene91614:CDS	30.0%
	GAGATTACTTCAATTGATAG+AGG	-	chr7.4:85184425-85184444	MS.gene91614:CDS	30.0%
	TCCAAATGGTACATTGTAAA+AGG	-	chr7.4:85183966-85183985	MS.gene91614:CDS	30.0%
	TGGACTTATGAAAATGTGAT+AGG	+	chr7.4:85183830-85183849	None:intergenic	30.0%
	TTATGGCAAAACAAAAACGA+TGG	-	chr7.4:85184256-85184275	MS.gene91614:CDS	30.0%
!	ATAACCCTTTTTCCAAAACT+TGG	-	chr7.4:85183591-85183610	MS.gene91614:CDS	30.0%
!	CAATTTCCAAAGTAGTTTTC+AGG	+	chr7.4:85184158-85184177	None:intergenic	30.0%
!	GATGATTTTACAAAGGAAGA+TGG	-	chr7.4:85184209-85184228	MS.gene91614:CDS	30.0%
!	TCCTTTTACAATGTACCATT+TGG	+	chr7.4:85183970-85183989	None:intergenic	30.0%
!	TGAGTCAGATGATTTTACAA+AGG	-	chr7.4:85184202-85184221	MS.gene91614:CDS	30.0%
!	TGTTTCCTTCGATAAAGTTT+TGG	-	chr7.4:85183476-85183495	MS.gene91614:CDS	30.0%
!	TTCAAGCAATTTTTCGTGAA+GGG	-	chr7.4:85184534-85184553	MS.gene91614:CDS	30.0%
!	TTTCAAGCAATTTTTCGTGA+AGG	-	chr7.4:85184533-85184552	MS.gene91614:CDS	30.0%
!	TTTTACAAAGGAAGATGGTT+TGG	-	chr7.4:85184214-85184233	MS.gene91614:CDS	30.0%
!!	GATAATTGGTTTGCAGAATT+AGG	+	chr7.4:85183183-85183202	None:intergenic	30.0%
!!	TCCAAAAAGGGTTCTAAAAA+AGG	+	chr7.4:85183744-85183763	None:intergenic	30.0%
!!!	AAATTGATTTTGGTTTCGGA+AGG	-	chr7.4:85184390-85184409	MS.gene91614:CDS	30.0%
!!!	AAGATTTGAGTTTTGGAACA+AGG	+	chr7.4:85183317-85183336	None:intergenic	30.0%
!!!	TCACTTTCTTTGACTTGTTT+TGG	-	chr7.4:85183211-85183230	MS.gene91614:CDS	30.0%
!!!	TCCTTTTTTAGAACCCTTTT+TGG	-	chr7.4:85183740-85183759	MS.gene91614:CDS	30.0%
	AAACGATGTTACCAGCTAAA+GGG	+	chr7.4:85183368-85183387	None:intergenic	35.0%
	AACAAGTCAAAGAAAGTGAG+AGG	+	chr7.4:85183210-85183229	None:intergenic	35.0%
	AATCTTTAAGCTTCTCACGT+TGG	+	chr7.4:85183850-85183869	None:intergenic	35.0%
	ACAAGTCAAAGAAAGTGAGA+GGG	+	chr7.4:85183209-85183228	None:intergenic	35.0%
	ATGATTGGTGAAGGAATTCA+AGG	-	chr7.4:85184329-85184348	MS.gene91614:CDS	35.0%
	TATTTGGCTATGATTGGTGA+AGG	-	chr7.4:85184320-85184339	MS.gene91614:CDS	35.0%
	TGTTTCCGTTTGTATAGCAA+AGG	-	chr7.4:85184046-85184065	MS.gene91614:CDS	35.0%
!	AAGGGTGTGATTGTGAATTT+GGG	+	chr7.4:85183278-85183297	None:intergenic	35.0%
!	CCAATGGAAAAACCAAGTTT+TGG	+	chr7.4:85183606-85183625	None:intergenic	35.0%
!	TATCGCCTTTGCTATACAAA+CGG	+	chr7.4:85184054-85184073	None:intergenic	35.0%
!	TTGAAAGGTGGTGTTGAATT+TGG	-	chr7.4:85184479-85184498	MS.gene91614:CDS	35.0%
!!	ATTGTTCGTGGATTTTGATG+TGG	+	chr7.4:85183149-85183168	None:intergenic	35.0%
!!	CCAAAACTTGGTTTTTCCAT+TGG	-	chr7.4:85183603-85183622	MS.gene91614:CDS	35.0%
!!	GAAGTAATCTCCACTTTTGA+AGG	+	chr7.4:85184416-85184435	None:intergenic	35.0%
!!	GAATCTGTTGATTCTAAGTG+AGG	+	chr7.4:85183546-85183565	None:intergenic	35.0%
!!	GAGAGTGAAGATTTGAGTTT+TGG	+	chr7.4:85183324-85183343	None:intergenic	35.0%
!!	TGGATTTTGATGTGGTTGTT+TGG	+	chr7.4:85183141-85183160	None:intergenic	35.0%
!!	TTTAGAACCCTTTTTGGAGA+GGG	-	chr7.4:85183746-85183765	MS.gene91614:CDS	35.0%
!!!	AATGGTTTTGGAGAATCAGA+AGG	+	chr7.4:85183393-85183412	None:intergenic	35.0%
!!!	TCTTTGACTTGTTTTGGCTA+AGG	-	chr7.4:85183217-85183236	MS.gene91614:CDS	35.0%
!!!	TTTTAGAACCCTTTTTGGAG+AGG	-	chr7.4:85183745-85183764	MS.gene91614:CDS	35.0%
	AAGGAAACTCCGTCATCATT+TGG	+	chr7.4:85183429-85183448	None:intergenic	40.0%
	AAGTGAGAGGGAGAGATAAT+TGG	+	chr7.4:85183197-85183216	None:intergenic	40.0%
	AATCTTGATCTGCAATCCAC+AGG	+	chr7.4:85184122-85184141	None:intergenic	40.0%
	AGAAGTAGATACGTTCACCT+GGG	+	chr7.4:85183248-85183267	None:intergenic	40.0%
	ATGGTGTGTGCTTATACCAA+TGG	+	chr7.4:85183622-85183641	None:intergenic	40.0%
	ATTCAAGGAATAGGAGTTGC+AGG	-	chr7.4:85184344-85184363	MS.gene91614:CDS	40.0%
	CAAACGATGTTACCAGCTAA+AGG	+	chr7.4:85183369-85183388	None:intergenic	40.0%
	CAACCATGTTCATCAAAGCT+TGG	-	chr7.4:85183664-85183683	MS.gene91614:CDS	40.0%
	CCAGTTCCTGAAAACTACTT+TGG	-	chr7.4:85184149-85184168	MS.gene91614:CDS	40.0%
	CTTTAGCTGGTAACATCGTT+TGG	-	chr7.4:85183367-85183386	MS.gene91614:CDS	40.0%
	GAGAAACTTTGCATTGTTCG+TGG	+	chr7.4:85183161-85183180	None:intergenic	40.0%
	GGTGAAGGAATTCAAGGAAT+AGG	-	chr7.4:85184335-85184354	MS.gene91614:CDS	40.0%
	GTGCTCCAAAACTTTATCGA+AGG	+	chr7.4:85183484-85183503	None:intergenic	40.0%
	GTGTGATTGTGAATTTGGGA+GGG	+	chr7.4:85183274-85183293	None:intergenic	40.0%
	TAGAAGTAGATACGTTCACC+TGG	+	chr7.4:85183249-85183268	None:intergenic	40.0%
	TGTTCTTGAGAAGCAAGTGA+TGG	-	chr7.4:85184505-85184524	MS.gene91614:CDS	40.0%
	TTCAAGGAATAGGAGTTGCA+GGG	-	chr7.4:85184345-85184364	MS.gene91614:CDS	40.0%
!	AGACCAAGCTTTGATGAACA+TGG	+	chr7.4:85183670-85183689	None:intergenic	40.0%
!	ATTTTCCGTCAAGAACAGCA+TGG	+	chr7.4:85183641-85183660	None:intergenic	40.0%
!	CGAACGGAATCCTTAACTTT+TGG	+	chr7.4:85183891-85183910	None:intergenic	40.0%
!	GAAGGGTGTGATTGTGAATT+TGG	+	chr7.4:85183279-85183298	None:intergenic	40.0%
!!	AAGTAGTTTTCAGGAACTGG+TGG	+	chr7.4:85184149-85184168	None:intergenic	40.0%
!!	ACAAAAGGGTGTTGTCCAAA+TGG	-	chr7.4:85183952-85183971	MS.gene91614:CDS	40.0%
!!	ATGGATAAGGGTGTTCTTGA+TGG	-	chr7.4:85184275-85184294	MS.gene91614:CDS	40.0%
!!	CCAAAGTAGTTTTCAGGAAC+TGG	+	chr7.4:85184152-85184171	None:intergenic	40.0%
!!	TGGATAAGGGTGTTCTTGAT+GGG	-	chr7.4:85184276-85184295	MS.gene91614:CDS	40.0%
!!	TTAGAACCCTTTTTGGAGAG+GGG	-	chr7.4:85183747-85183766	MS.gene91614:CDS	40.0%
!!!	TAAAGGGAGGAAGTTTTGGA+GGG	+	chr7.4:85183352-85183371	None:intergenic	40.0%
	ACACACCATGCTGTTCTTGA+CGG	-	chr7.4:85183633-85183652	MS.gene91614:CDS	45.0%
	ACTACTCCCCTCTCCAAAAA+GGG	+	chr7.4:85183756-85183775	None:intergenic	45.0%
	AGTTAAGGATTCCGTTCGAG+TGG	-	chr7.4:85183893-85183912	MS.gene91614:CDS	45.0%
	CAAAACTTCCTCCCTTTAGC+TGG	-	chr7.4:85183354-85183373	MS.gene91614:CDS	45.0%
	CGAGACAAAGAAGCTTCTTG+TGG	+	chr7.4:85183513-85183532	None:intergenic	45.0%
	GGTGTGATTGTGAATTTGGG+AGG	+	chr7.4:85183275-85183294	None:intergenic	45.0%
	TACTACTCCCCTCTCCAAAA+AGG	+	chr7.4:85183757-85183776	None:intergenic	45.0%
	TTTCGGAAGGCCTTCAAAAG+TGG	-	chr7.4:85184403-85184422	MS.gene91614:CDS	45.0%
	TTTGGAGAGGGGAGTAGTAA+AGG	-	chr7.4:85183758-85183777	MS.gene91614:CDS	45.0%
!	CAGCTAAAGGGAGGAAGTTT+TGG	+	chr7.4:85183356-85183375	None:intergenic	45.0%
!!	CTAAAGGGAGGAAGTTTTGG+AGG	+	chr7.4:85183353-85183372	None:intergenic	45.0%
!!	TTTTGGCTAAGGTTTCACCC+AGG	-	chr7.4:85183228-85183247	MS.gene91614:CDS	45.0%
	AGTTGCAGGGTCAACAAGGT+TGG	-	chr7.4:85184358-85184377	MS.gene91614:CDS	50.0%
	CGATGTTACCAGCTAAAGGG+AGG	+	chr7.4:85183365-85183384	None:intergenic	50.0%
	GAGCGTGAATACCACTCGAA+CGG	+	chr7.4:85183907-85183926	None:intergenic	50.0%
	GTTGCAGGGTCAACAAGGTT+GGG	-	chr7.4:85184359-85184378	MS.gene91614:CDS	50.0%
	TAGGAGTTGCAGGGTCAACA+AGG	-	chr7.4:85184354-85184373	MS.gene91614:CDS	50.0%
	GTGGTATTCACGCTCACACG+CGG	-	chr7.4:85183912-85183931	MS.gene91614:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	85183129	85184571	85183129	ID=MS.gene91614
chr7.4	mRNA	85183129	85184571	85183129	ID=MS.gene91614.t1;Parent=MS.gene91614
chr7.4	exon	85183129	85184571	85183129	ID=MS.gene91614.t1.exon1;Parent=MS.gene91614.t1
chr7.4	CDS	85183129	85184571	85183129	ID=cds.MS.gene91614.t1;Parent=MS.gene91614.t1

Gene Sequence

>MS.gene91614

ATGGCTTCTCCAAACAACCACATCAAAATCCACGAACAATGCAAAGTTTCTCCTAATTCTGCAAACCAATTATCTCTCCCTCTCACTTTCTTTGACTTGTTTTGGCTAAGGTTTCACCCAGGTGAACGTATCTACTTCTACACCCTCCCAAATTCACAATCACACCCTTCTTTCTTTTTTGAAAACCTTGTTCCAAAACTCAAATCTTCACTCTCTTTAACCCTCCAAAACTTCCTCCCTTTAGCTGGTAACATCGTTTGGCCTTCTGATTCTCCAAAACCATTCATCAAATTCAATCCAAATGATGACGGAGTTTCCTTGCTCTTAACACAATGTAATGACGATGATGTTTCCTTCGATAAAGTTTTGGAGCACAATTCACCACAAGAAGCTTCTTTGTCTCGTTCTTTTGTACCTCACTTAGAATCAACAGATTCTTTTGCTTCTATTATTTCAATTCAGATAACCCTTTTTCCAAAACTTGGTTTTTCCATTGGTATAAGCACACACCATGCTGTTCTTGACGGAAAATCTTCAACCATGTTCATCAAAGCTTGGTCTTCTATATCTAAATCTTTTCAAGAAGAAGAAATGAAACCACCAAACTTAGATCCTTTTTTAGAACCCTTTTTGGAGAGGGGAGTAGTAAAGGATCCAAATAATTTTGAGAGTCTGTTCATAAATAATTGGGACAAAATCCTATCACATTTTCATAAGTCCAACGTGAGAAGCTTAAAGATTATGTCAGACTCATTTGAACCAAAAGTTAAGGATTCCGTTCGAGTGGTATTCACGCTCACACGCGGAGATTTAGAGAAATTAAACAAAAGGGTGTTGTCCAAATGGTACATTGTAAAAGGAGCACAAGAACATGAACAACCTAAGAAATTGTCAACTTTTGTTCTTACATGTGCTTATGTTTCCGTTTGTATAGCAAAGGCGATACAACAATCCGAAAGCGATAAAAAACAGAAATTTTCTTTTAGTTTTCCTGTGGATTGCAGATCAAGATTAGATCCACCAGTTCCTGAAAACTACTTTGGAAATTGTGTATGTAATCATATAGTTGATACTGAGTCAGATGATTTTACAAAGGAAGATGGTTTGGTAATTGTTGCTAATAAAATTTATGGCAAAACAAAAACGATGGATAAGGGTGTTCTTGATGGGATAGAGACATTGACGTCTAAATATTTGGCTATGATTGGTGAAGGAATTCAAGGAATAGGAGTTGCAGGGTCAACAAGGTTGGGAGTATATGAAATTGATTTTGGTTTCGGAAGGCCTTCAAAAGTGGAGATTACTTCAATTGATAGAGGATTAACTATTGGTTTAACAGAAAGTAATGATTTGAAAGGTGGTGTTGAATTTGGACTTGTTCTTGAGAAGCAAGTGATGGATTTGTTTCAAGCAATTTTTCGTGAAGGGTTGTATTTTGATTGA

Protein sequence

>MS.gene91614.t1

MASPNNHIKIHEQCKVSPNSANQLSLPLTFFDLFWLRFHPGERIYFYTLPNSQSHPSFFFENLVPKLKSSLSLTLQNFLPLAGNIVWPSDSPKPFIKFNPNDDGVSLLLTQCNDDDVSFDKVLEHNSPQEASLSRSFVPHLESTDSFASIISIQITLFPKLGFSIGISTHHAVLDGKSSTMFIKAWSSISKSFQEEEMKPPNLDPFLEPFLERGVVKDPNNFESLFINNWDKILSHFHKSNVRSLKIMSDSFEPKVKDSVRVVFTLTRGDLEKLNKRVLSKWYIVKGAQEHEQPKKLSTFVLTCAYVSVCIAKAIQQSESDKKQKFSFSFPVDCRSRLDPPVPENYFGNCVCNHIVDTESDDFTKEDGLVIVANKIYGKTKTMDKGVLDGIETLTSKYLAMIGEGIQGIGVAGSTRLGVYEIDFGFGRPSKVEITSIDRGLTIGLTESNDLKGGVEFGLVLEKQVMDLFQAIFREGLYFD