AlfalfaGEDB | MS.gene91613

M. sativa cultivar XinJiangDaYe / MS.gene91613

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016740 GO:0016746 GO:0016747
PFAM: PF02458.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91613.t1	XP_013447578.1	80.2	484	91	4	1	480	1	483	2.70E-217	764.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91613.t1	Q9LJB4	38.0	484	257	12	1	477	1	448	3.3e-78	293.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91613.t1	B4Y0U1	80.2	484	91	4	1	480	1	483	2.0e-217	764.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene03202	MS.gene91613	PPI
MS.gene91613	MS.gene50356	PPI
MS.gene91613	MS.gene87536	PPI
MS.gene91613	MS.gene56797	PPI
MS.gene012360	MS.gene91613	PPI
MS.gene35744	MS.gene91613	PPI
MS.gene91613	MS.gene99218	PPI
MS.gene028999	MS.gene91613	PPI
MS.gene028998	MS.gene91613	PPI
MS.gene91609	MS.gene91613	PPI
MS.gene56795	MS.gene91613	PPI
MS.gene021915	MS.gene91613	PPI
MS.gene50355	MS.gene91613	PPI
MS.gene011798	MS.gene91613	PPI
MS.gene91611	MS.gene91613	PPI
MS.gene48584	MS.gene91613	PPI
MS.gene021904	MS.gene91613	PPI
MS.gene073299	MS.gene91613	PPI
MS.gene91613	MS.gene02573	PPI
MS.gene021916	MS.gene91613	PPI
MS.gene32261	MS.gene91613	PPI
MS.gene91613	MS.gene35740	PPI
MS.gene99219	MS.gene91613	PPI
MS.gene91613	MS.gene87577	PPI
MS.gene44463	MS.gene91613	PPI
MS.gene91613	MS.gene021905	PPI
MS.gene066657	MS.gene91613	PPI
MS.gene91613	MS.gene070270	PPI
MS.gene56798	MS.gene91613	PPI
MS.gene069423	MS.gene91613	PPI
MS.gene78818	MS.gene91613	PPI
MS.gene91613	MS.gene50382	PPI
MS.gene069422	MS.gene91613	PPI
MS.gene91613	MS.gene91614	PPI
MS.gene44643	MS.gene91613	PPI
MS.gene91613	MS.gene87191	PPI
MS.gene91613	MS.gene48584	PPI
MS.gene31545	MS.gene91613	PPI
MS.gene44633	MS.gene91613	PPI
MS.gene91613	MS.gene87496	PPI
MS.gene91613	MS.gene016477	PPI
MS.gene91613	MS.gene021913	PPI
MS.gene91614	MS.gene91613	PPI
MS.gene91612	MS.gene91613	PPI
MS.gene50382	MS.gene91613	PPI
MS.gene91597	MS.gene91613	PPI
MS.gene31546	MS.gene91613	PPI
MS.gene91613	MS.gene028994	PPI
MS.gene91613	MS.gene91596	PPI
MS.gene35741	MS.gene91613	PPI
MS.gene021914	MS.gene91613	PPI
MS.gene90496	MS.gene91613	PPI
MS.gene91598	MS.gene91613	PPI
MS.gene021910	MS.gene91613	PPI
MS.gene91613	MS.gene000763	PPI
MS.gene028996	MS.gene91613	PPI
MS.gene000763	MS.gene91613	PPI
MS.gene28251	MS.gene91613	PPI
MS.gene35745	MS.gene91613	PPI
MS.gene91613	MS.gene043350	PPI
MS.gene011801	MS.gene91613	PPI
MS.gene000764	MS.gene91613	PPI
MS.gene91613	MS.gene56798	PPI
MS.gene91615	MS.gene91613	PPI
MS.gene48582	MS.gene91613	PPI
MS.gene38977	MS.gene91613	PPI
MS.gene90491	MS.gene91613	PPI
MS.gene50379	MS.gene91613	PPI
MS.gene91613	MS.gene50384	PPI
MS.gene91613	MS.gene96337	PPI
MS.gene021906	MS.gene91613	PPI
MS.gene91613	MS.gene066658	PPI
MS.gene87532	MS.gene91613	PPI
MS.gene99223	MS.gene91613	PPI
MS.gene91613	MS.gene070019	PPI
MS.gene91613	MS.gene021917	PPI
MS.gene46265	MS.gene91613	PPI
MS.gene90492	MS.gene91613	PPI
MS.gene063868	MS.gene91613	PPI
MS.gene066658	MS.gene91613	PPI
MS.gene48581	MS.gene91613	PPI
MS.gene48585	MS.gene91613	PPI
MS.gene011837	MS.gene91613	PPI
MS.gene066634	MS.gene91613	PPI
MS.gene043346	MS.gene91613	PPI
MS.gene91599	MS.gene91613	PPI
MS.gene99221	MS.gene91613	PPI
MS.gene91613	MS.gene56799	PPI
MS.gene49853	MS.gene91613	PPI
MS.gene063865	MS.gene91613	PPI
MS.gene007831	MS.gene91613	PPI
MS.gene44642	MS.gene91613	PPI
MS.gene91613	MS.gene44630	PPI
MS.gene91613	MS.gene069422	PPI
MS.gene91613	MS.gene069767	PPI
MS.gene56796	MS.gene91613	PPI
MS.gene069767	MS.gene91613	PPI
MS.gene004218	MS.gene91613	PPI
MS.gene44644	MS.gene91613	PPI
MS.gene011796	MS.gene91613	PPI
MS.gene91613	MS.gene011716	PPI
MS.gene87537	MS.gene91613	PPI
MS.gene91613	MS.gene28251	PPI
MS.gene99222	MS.gene91613	PPI
MS.gene91613	MS.gene91595	PPI
MS.gene60208	MS.gene91613	PPI
MS.gene90493	MS.gene91613	PPI
MS.gene91613	MS.gene016532	PPI
MS.gene91613	MS.gene043352	PPI
MS.gene032349	MS.gene91613	PPI
MS.gene91613	MS.gene44647	PPI
MS.gene91613	MS.gene066701	PPI
MS.gene013787	MS.gene91613	PPI
MS.gene066656	MS.gene91613	PPI
MS.gene91613	MS.gene021909	PPI
MS.gene91613	MS.gene50355	PPI
MS.gene31542	MS.gene91613	PPI
MS.gene91613	MS.gene91612	PPI
MS.gene066660	MS.gene91613	PPI
MS.gene31540	MS.gene91613	PPI
MS.gene44640	MS.gene91613	PPI
MS.gene91613	MS.gene011799	PPI
MS.gene91613	MS.gene002544	PPI
MS.gene91613	MS.gene50420	PPI
MS.gene043348	MS.gene91613	PPI
MS.gene91613	MS.gene064101	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91613.t1	MTR_7g013850	79.959	484	92	4	1	480	1	483	0.0	770
MS.gene91613.t1	MTR_7g014200	58.884	484	176	12	1	477	1	468	0.0	533
MS.gene91613.t1	MTR_7g014160	58.038	479	184	11	5	477	6	473	0.0	533
MS.gene91613.t1	MTR_7g014610	58.592	483	186	9	1	477	1	475	0.0	516
MS.gene91613.t1	MTR_7g014620	52.711	461	182	7	19	477	6	432	5.57e-150	435
MS.gene91613.t1	MTR_7g014630	56.811	301	117	7	6	301	7	299	2.23e-108	326
MS.gene91613.t1	MTR_6g015315	39.149	470	262	10	9	477	6	452	1.95e-102	314
MS.gene91613.t1	MTR_7g014330	39.749	478	255	15	9	477	3	456	1.07e-100	310
MS.gene91613.t1	MTR_6g015320	37.190	484	267	12	1	477	1	454	5.17e-99	305
MS.gene91613.t1	MTR_7g014230	38.622	479	263	12	9	479	5	460	5.57e-99	305
MS.gene91613.t1	MTR_6g015830	39.794	485	243	14	9	477	9	460	6.51e-99	305
MS.gene91613.t1	MTR_6g015850	39.104	491	251	16	1	477	1	457	9.78e-99	305
MS.gene91613.t1	MTR_7g014250	38.994	477	260	14	9	477	3	456	1.19e-98	304
MS.gene91613.t1	MTR_6g015815	41.138	457	238	12	25	477	31	460	2.55e-96	298
MS.gene91613.t1	MTR_3g028210	35.464	485	270	16	8	477	8	464	4.37e-75	244
MS.gene91613.t1	MTR_7g014180	70.391	179	46	5	1	178	1	173	3.07e-74	231
MS.gene91613.t1	MTR_7g014670	55.959	193	83	2	289	480	71	262	2.70e-68	219
MS.gene91613.t1	MTR_2g089765	32.515	489	281	15	11	477	21	482	3.48e-67	223
MS.gene91613.t1	MTR_7g014360	34.152	407	218	12	78	477	23	386	9.64e-65	214
MS.gene91613.t1	MTR_2g089670	32.181	463	275	14	26	477	43	477	2.16e-61	208
MS.gene91613.t1	MTR_2g089650	28.514	498	300	15	1	477	1	463	7.05e-58	198
MS.gene91613.t1	MTR_3g087640	29.577	497	288	11	9	477	10	472	1.24e-57	198
MS.gene91613.t1	MTR_2g089735	31.614	446	271	11	42	477	40	461	1.29e-57	197
MS.gene91613.t1	MTR_7g015860	30.832	493	281	17	8	477	5	460	2.04e-55	192
MS.gene91613.t1	MTR_8g012750	31.210	471	279	15	26	475	24	470	3.88e-54	189
MS.gene91613.t1	MTR_2g089755	28.541	466	290	13	28	477	37	475	2.26e-52	184
MS.gene91613.t1	MTR_6g015310	30.617	405	208	12	51	455	2	333	2.29e-48	169
MS.gene91613.t1	MTR_7g014310	29.730	333	165	9	130	460	41	306	2.01e-39	145
MS.gene91613.t1	MTR_2g089665	25.164	457	201	14	11	454	7	335	1.72e-25	107
MS.gene91613.t1	MTR_3g435300	30.000	280	164	9	9	276	4	263	6.12e-25	104
MS.gene91613.t1	MTR_4g007540	24.017	483	289	21	8	470	3	427	1.80e-21	97.1
MS.gene91613.t1	MTR_8g075610	24.190	401	233	16	44	429	30	374	1.38e-20	94.4
MS.gene91613.t1	MTR_3g049150	24.374	439	268	18	63	478	51	448	1.44e-18	88.6
MS.gene91613.t1	MTR_7g086000	24.200	500	305	19	7	477	3	457	3.87e-18	87.0
MS.gene91613.t1	MTR_3g070170	24.615	455	270	18	8	433	12	422	4.09e-18	87.0
MS.gene91613.t1	MTR_7g014210	38.318	107	64	2	335	439	24	130	1.62e-17	80.5
MS.gene91613.t1	MTR_2g064495	24.406	463	272	23	32	475	28	431	1.70e-17	85.1
MS.gene91613.t1	MTR_2g105330	24.729	461	273	23	32	475	28	431	4.21e-17	84.0
MS.gene91613.t1	MTR_7g014205	37.615	109	56	2	332	439	17	114	1.01e-16	76.6
MS.gene91613.t1	MTR_3g026950	23.448	435	270	17	64	473	51	447	1.24e-16	82.4
MS.gene91613.t1	MTR_8g096530	24.599	374	233	10	61	429	53	382	3.34e-16	81.3
MS.gene91613.t1	MTR_3g088420	23.602	483	261	16	17	467	14	420	5.11e-16	80.5
MS.gene91613.t1	MTR_4g022800	22.622	473	287	18	17	467	14	429	8.06e-16	80.1
MS.gene91613.t1	MTR_4g022720	23.158	475	275	18	17	467	14	422	1.97e-15	78.6
MS.gene91613.t1	MTR_7g085930	22.403	491	329	15	6	477	4	461	7.02e-15	77.0
MS.gene91613.t1	MTR_7g085990	23.510	302	200	9	50	349	42	314	7.83e-14	73.9
MS.gene91613.t1	MTR_3g101970	24.208	442	261	20	8	433	6	389	1.69e-13	72.8
MS.gene91613.t1	MTR_7g014340	53.521	71	26	2	9	75	3	70	4.90e-13	65.5
MS.gene91613.t1	MTR_4g022825	21.798	445	262	16	28	446	24	408	3.36e-12	68.6
MS.gene91613.t1	MTR_8g467580	26.107	429	243	22	17	429	15	385	3.67e-12	68.6
MS.gene91613.t1	MTR_8g079970	23.061	477	274	18	17	470	15	421	5.29e-12	68.2
MS.gene91613.t1	MTR_8g079950	24.043	470	278	17	17	470	15	421	9.61e-12	67.4
MS.gene91613.t1	MTR_7g014220	56.604	53	20	1	25	77	15	64	5.62e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91613.t1	AT3G29590	38.223	484	256	12	1	477	1	448	9.55e-90	281
MS.gene91613.t1	AT3G29670	38.622	479	255	12	8	478	3	450	2.00e-87	275
MS.gene91613.t1	AT5G39080	38.689	473	262	15	1	467	1	451	2.22e-87	275
MS.gene91613.t1	AT5G39050	37.474	483	264	15	8	477	10	467	1.58e-86	273
MS.gene91613.t1	AT3G29635	37.603	484	256	15	8	477	3	454	1.24e-85	271
MS.gene91613.t1	AT5G39090	36.632	475	265	14	6	477	6	447	4.89e-83	264
MS.gene91613.t1	AT1G03940	31.919	495	272	13	9	477	9	464	5.20e-71	233
MS.gene91613.t1	AT5G61160	34.038	473	276	14	8	473	3	446	1.24e-69	229
MS.gene91613.t1	AT3G29680	34.874	476	266	14	6	473	4	443	1.70e-68	226
MS.gene91613.t1	AT1G03495	31.720	372	213	12	1	360	1	343	2.95e-45	162
MS.gene91613.t1	AT5G48930	24.431	483	297	20	8	475	3	432	6.64e-20	92.4
MS.gene91613.t1	AT3G29720	33.161	193	106	8	99	282	10	188	1.58e-16	79.0
MS.gene91613.t1	AT3G50280	22.511	462	287	16	19	468	18	420	4.86e-15	77.4
MS.gene91613.t1	AT5G57840	23.142	471	291	16	25	477	21	438	7.82e-15	77.0
MS.gene91613.t1	AT3G29690	29.801	151	100	2	328	477	33	178	1.43e-14	72.4
MS.gene91613.t1	AT1G28680	21.409	369	246	9	67	432	62	389	1.56e-13	72.8
MS.gene91613.t1	AT2G39980	23.503	451	308	14	1	433	1	432	2.16e-13	72.8
MS.gene91613.t1	AT1G24430	22.695	423	241	16	67	471	62	416	9.79e-13	70.5
MS.gene91613.t1	AT5G07850	23.714	447	285	16	1	433	1	405	1.27e-12	70.1
MS.gene91613.t1	AT5G23970	22.614	482	265	20	16	470	11	411	2.59e-12	68.9
MS.gene91613.t1	AT5G41040	23.310	429	260	20	61	473	63	438	3.09e-12	68.9
MS.gene91613.t1	AT5G41040	23.310	429	260	20	61	473	79	454	4.39e-12	68.6
MS.gene91613.t1	AT4G29250	23.836	365	215	14	43	396	49	361	6.03e-12	68.2
MS.gene91613.t1	AT4G31910	22.823	333	233	7	60	389	57	368	8.03e-12	67.8
MS.gene91613.t1	AT3G48720	21.583	417	270	14	67	474	61	429	4.56e-11	65.1

Find 73 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAGACTCTCAAAATTATT+TGG	0.163672	7.4:+85177363	None:intergenic
GAAGGGTGTGATTGTGAATT+TGG	0.182607	7.4:+85177870	None:intergenic
AGAGTCTGTTCATAAATAAT+TGG	0.191926	7.4:-85177350	MS.gene91613:CDS
GACATTGACGTCTAAATATT+TGG	0.199250	7.4:-85176842	MS.gene91613:CDS
TCACTTTCTTTGACTTGTTT+TGG	0.206968	7.4:-85177935	MS.gene91613:CDS
TTGAAAGGTGGTGTTGAATT+TGG	0.214046	7.4:-85176667	MS.gene91613:CDS
GAGAGTGAAGATTTGAGTTT+TGG	0.216722	7.4:+85177825	None:intergenic
GATAATTGGTTTGCAGAATT+AGG	0.221219	7.4:+85177966	None:intergenic
CAGCTAAAGGGAGGAAGTTT+TGG	0.225428	7.4:+85177793	None:intergenic
ACAAAAGTTGACAATTTCTT+AGG	0.235091	7.4:+85177138	None:intergenic
TACTACTCCCCTCTCCAAAA+AGG	0.248355	7.4:+85177392	None:intergenic
ATTGTTGCTAATAAAATTTA+TGG	0.257747	7.4:-85176907	MS.gene91613:CDS
GGAGTATATGAAATTGATTT+TGG	0.266147	7.4:-85176766	MS.gene91613:CDS
TTGAATTTGATGAATGGTTT+TGG	0.270000	7.4:+85177744	None:intergenic
CGAACGGAATCCTTAACTTT+TGG	0.284718	7.4:+85177258	None:intergenic
AAGTGAGAGGGAGAGATAAT+TGG	0.293242	7.4:+85177952	None:intergenic
TCCAAATCGTACATTGTAAA+AGG	0.310203	7.4:-85177180	MS.gene91613:CDS
TGTTTCCTTCGATAAAGTTT+TGG	0.341922	7.4:-85177670	MS.gene91613:CDS
CTTTAGCTGGTAACATCGTT+TGG	0.362068	7.4:-85177779	MS.gene91613:CDS
TCTAAATATTTGGCTATGAT+TGG	0.368222	7.4:-85176832	MS.gene91613:CDS
ATTGATAGAGGATTAACTAT+TGG	0.368461	7.4:-85176709	MS.gene91613:CDS
CTCATTTGAACCAAAAGTTA+AGG	0.369807	7.4:-85177268	MS.gene91613:CDS
TGGACTTATGAAAATGTGAT+AGG	0.386400	7.4:+85177319	None:intergenic
AAGGAAACTCCGTCATCATT+TGG	0.395044	7.4:+85177720	None:intergenic
ATGGATAAGGGTGTTCTTGA+TGG	0.403109	7.4:-85176871	MS.gene91613:CDS
CCAGTTCCTGAAAACTACTT+TGG	0.404805	7.4:-85176997	MS.gene91613:CDS
ATTTAGAGAAATTAAACAAA+AGG	0.406967	7.4:-85177209	MS.gene91613:CDS
ATTCAAGGAATAGGAGTTGC+AGG	0.408252	7.4:-85176802	MS.gene91613:CDS
AAGGGTGTGATTGTGAATTT+GGG	0.425297	7.4:+85177871	None:intergenic
GGTGAAGGAATTCAAGGAAT+AGG	0.425888	7.4:-85176811	MS.gene91613:CDS
GAGTCTGTTCATAAATAATT+GGG	0.449772	7.4:-85177349	MS.gene91613:CDS
GAGATTACTTCAATTGATAG+AGG	0.456941	7.4:-85176721	MS.gene91613:CDS
CAAAACTTCCTCCCTTTAGC+TGG	0.462356	7.4:-85177792	MS.gene91613:CDS
TTTAGAGAAATTAAACAAAA+GGG	0.466166	7.4:-85177208	MS.gene91613:CDS
TTTGGATTGAATTTGATGAA+TGG	0.473082	7.4:+85177738	None:intergenic
AACAAGTCAAAGAAAGTGAG+AGG	0.479248	7.4:+85177939	None:intergenic
TTTGGAGAGGGGAGTAGTAA+AGG	0.482127	7.4:-85177388	MS.gene91613:CDS
ACACACCATGCTGTTCTTGA+CGG	0.482364	7.4:-85177513	MS.gene91613:CDS
GAGACACTTTGCATTGTTCG+TGG	0.482647	7.4:+85177988	None:intergenic
CAACCATGTTCATCAAAGCT+TGG	0.484613	7.4:-85177482	MS.gene91613:CDS
TATCGCCTTTGCTATACAAA+CGG	0.491635	7.4:+85177095	None:intergenic
CAAACGATGTTACCAGCTAA+AGG	0.503485	7.4:+85177780	None:intergenic
ATGATTGGTGAAGGAATTCA+AGG	0.503765	7.4:-85176817	MS.gene91613:CDS
AGTTGCAGGGTCAACAAGGT+TGG	0.506239	7.4:-85176788	MS.gene91613:CDS
TTTCGGAAGGCCTTCAAAAG+TGG	0.513160	7.4:-85176743	MS.gene91613:CDS
AAACGATGTTACCAGCTAAA+GGG	0.515290	7.4:+85177781	None:intergenic
ACAGAAAGTAATGATTTGAA+AGG	0.524129	7.4:-85176682	MS.gene91613:CDS
GTTGCAGGGTCAACAAGGTT+GGG	0.534201	7.4:-85176787	MS.gene91613:CDS
TGGATAAGGGTGTTCTTGAT+GGG	0.534950	7.4:-85176870	MS.gene91613:CDS
ATTACATTGTGTTAAGAGCA+AGG	0.536608	7.4:+85177701	None:intergenic
GAAAGTAATGATTTGAAAGG+TGG	0.539031	7.4:-85176679	MS.gene91613:CDS
TGTTCTTGAGAAGCAAGTGA+TGG	0.541641	7.4:-85176641	MS.gene91613:CDS
TGTTTCCGTTTGTATAGCAA+AGG	0.550669	7.4:-85177100	MS.gene91613:CDS
AATCTTGATCTGCAATCCAC+AGG	0.565697	7.4:+85177027	None:intergenic
GTGCTCCAAAACTTTATCGA+AGG	0.566459	7.4:+85177665	None:intergenic
TATTTGGCTATGATTGGTGA+AGG	0.577286	7.4:-85176826	MS.gene91613:CDS
TAGAAGTAGATACGTTCACC+TGG	0.583143	7.4:+85177900	None:intergenic
GAATCTGTTGATTCTAAGTG+AGG	0.596592	7.4:+85177603	None:intergenic
AATCTTTAAGCTTCTCACGT+TGG	0.603971	7.4:+85177299	None:intergenic
GTGTGATTGTGAATTTGGGA+GGG	0.604440	7.4:+85177875	None:intergenic
AAATTCAATCCAAATGATGA+CGG	0.606900	7.4:-85177729	MS.gene91613:CDS
AGACCAAGCTTTGATGAACA+TGG	0.608686	7.4:+85177479	None:intergenic
AGTTAAGGATTCCGTTCGAG+TGG	0.609297	7.4:-85177253	MS.gene91613:CDS
GGTGTGATTGTGAATTTGGG+AGG	0.618307	7.4:+85177874	None:intergenic
ACAAGTCAAAGAAAGTGAGA+GGG	0.619012	7.4:+85177940	None:intergenic
TAGGAGTTGCAGGGTCAACA+AGG	0.623066	7.4:-85176792	MS.gene91613:CDS
CGAGACAAAGAAGCTTCTTG+TGG	0.646679	7.4:+85177636	None:intergenic
ATGGTGTGTGCTTATACCAA+TGG	0.657084	7.4:+85177527	None:intergenic
TTCAAGGAATAGGAGTTGCA+GGG	0.658447	7.4:-85176801	MS.gene91613:CDS
CGATGTTACCAGCTAAAGGG+AGG	0.666686	7.4:+85177784	None:intergenic
GAGCGTGAATACCACTCGAA+CGG	0.670435	7.4:+85177242	None:intergenic
GTGGTATTCACGCTCACACG+CGG	0.681785	7.4:-85177234	MS.gene91613:CDS
AGAAGTAGATACGTTCACCT+GGG	0.704297	7.4:+85177901	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTGTTGCTAATAAAATTTA+TGG	-	chr7.4:85177707-85177726	MS.gene91613:CDS	15.0%
!!	ATTTAGAGAAATTAAACAAA+AGG	-	chr7.4:85177405-85177424	MS.gene91613:CDS	15.0%
!!	TTTAGAGAAATTAAACAAAA+GGG	-	chr7.4:85177406-85177425	MS.gene91613:CDS	15.0%
!!	AAAACAAAAACAATGGATAA+GGG	-	chr7.4:85177731-85177750	MS.gene91613:CDS	20.0%
!!!	TATGAAATTGATTTTGGTTT+CGG	-	chr7.4:85177854-85177873	MS.gene91613:CDS	20.0%
!	AAATTCAATCCAAATGATGA+CGG	-	chr7.4:85176885-85176904	MS.gene91613:CDS	25.0%
!	AACAGACTCTCAAAATTATT+TGG	+	chr7.4:85177254-85177273	None:intergenic	25.0%
!	ACAAAAGTTGACAATTTCTT+AGG	+	chr7.4:85177479-85177498	None:intergenic	25.0%
!	ACAGAAAGTAATGATTTGAA+AGG	-	chr7.4:85177932-85177951	MS.gene91613:CDS	25.0%
!	AGAGTCTGTTCATAAATAAT+TGG	-	chr7.4:85177264-85177283	MS.gene91613:CDS	25.0%
!	ATTGATAGAGGATTAACTAT+TGG	-	chr7.4:85177905-85177924	MS.gene91613:CDS	25.0%
!	CAAAACAAAAACAATGGATA+AGG	-	chr7.4:85177730-85177749	MS.gene91613:CDS	25.0%
!	GAGTCTGTTCATAAATAATT+GGG	-	chr7.4:85177265-85177284	MS.gene91613:CDS	25.0%
!	TCTAAAAAAGGATCTAAGTT+TGG	+	chr7.4:85177200-85177219	None:intergenic	25.0%
!	TCTAAATATTTGGCTATGAT+TGG	-	chr7.4:85177782-85177801	MS.gene91613:CDS	25.0%
!	TTATGGCAAAACAAAAACAA+TGG	-	chr7.4:85177724-85177743	MS.gene91613:CDS	25.0%
!	TTTGGATTGAATTTGATGAA+TGG	+	chr7.4:85176879-85176898	None:intergenic	25.0%
!!	AGGTTTTCAAAAAAGAAAGA+AGG	+	chr7.4:85176765-85176784	None:intergenic	25.0%
!!	GGTTTTCAAAAAAGAAAGAA+GGG	+	chr7.4:85176764-85176783	None:intergenic	25.0%
!!	TTGAATTTGATGAATGGTTT+TGG	+	chr7.4:85176873-85176892	None:intergenic	25.0%
!!!	AAAAACCAAGTTTTGGAAAA+AGG	+	chr7.4:85177067-85177086	None:intergenic	25.0%
!!!	AAAACCAAGTTTTGGAAAAA+GGG	+	chr7.4:85177066-85177085	None:intergenic	25.0%
!!!	ATTTTCTTTTAGTTTTCCTG+TGG	-	chr7.4:85177571-85177590	MS.gene91613:CDS	25.0%
!!!	GGAGTATATGAAATTGATTT+TGG	-	chr7.4:85177848-85177867	MS.gene91613:CDS	25.0%
!!!	TTTCTGTTTTTTATCGCTTT+CGG	+	chr7.4:85177552-85177571	None:intergenic	25.0%
	AAAAAAGGATCTAAGTTTGG+TGG	+	chr7.4:85177197-85177216	None:intergenic	30.0%
	ATTACATTGTGTTAAGAGCA+AGG	+	chr7.4:85176916-85176935	None:intergenic	30.0%
	CTCATTTGAACCAAAAGTTA+AGG	-	chr7.4:85177346-85177365	MS.gene91613:CDS	30.0%
	GAAAGTAATGATTTGAAAGG+TGG	-	chr7.4:85177935-85177954	MS.gene91613:CDS	30.0%
	GACATTGACGTCTAAATATT+TGG	-	chr7.4:85177772-85177791	MS.gene91613:CDS	30.0%
	GAGATTACTTCAATTGATAG+AGG	-	chr7.4:85177893-85177912	MS.gene91613:CDS	30.0%
	TCCAAATCGTACATTGTAAA+AGG	-	chr7.4:85177434-85177453	MS.gene91613:CDS	30.0%
	TGGACTTATGAAAATGTGAT+AGG	+	chr7.4:85177298-85177317	None:intergenic	30.0%
!	ATAACCCTTTTTCCAAAACT+TGG	-	chr7.4:85177059-85177078	MS.gene91613:CDS	30.0%
!	CAATTTCCAAAGTAGTTTTC+AGG	+	chr7.4:85177626-85177645	None:intergenic	30.0%
!	GATGATTTTACAAAGGAAGA+TGG	-	chr7.4:85177677-85177696	MS.gene91613:CDS	30.0%
!	TCCTTTTACAATGTACGATT+TGG	+	chr7.4:85177438-85177457	None:intergenic	30.0%
!	TGAGTCAGATGATTTTACAA+AGG	-	chr7.4:85177670-85177689	MS.gene91613:CDS	30.0%
!	TGTTTCCTTCGATAAAGTTT+TGG	-	chr7.4:85176944-85176963	MS.gene91613:CDS	30.0%
!	TTCAAGCAATTTTTCGTGAA+GGG	-	chr7.4:85178002-85178021	MS.gene91613:CDS	30.0%
!	TTTCAAGCAATTTTTCGTGA+AGG	-	chr7.4:85178001-85178020	MS.gene91613:CDS	30.0%
!	TTTTACAAAGGAAGATGGTT+TGG	-	chr7.4:85177682-85177701	MS.gene91613:CDS	30.0%
!!	GATAATTGGTTTGCAGAATT+AGG	+	chr7.4:85176651-85176670	None:intergenic	30.0%
!!	TCCAAAAAGGGTTCTAAAAA+AGG	+	chr7.4:85177212-85177231	None:intergenic	30.0%
!!!	AAATTGATTTTGGTTTCGGA+AGG	-	chr7.4:85177858-85177877	MS.gene91613:CDS	30.0%
!!!	AAGATTTGAGTTTTGGAACA+AGG	+	chr7.4:85176785-85176804	None:intergenic	30.0%
!!!	TCACTTTCTTTGACTTGTTT+TGG	-	chr7.4:85176679-85176698	MS.gene91613:CDS	30.0%
!!!	TCCTTTTTTAGAACCCTTTT+TGG	-	chr7.4:85177208-85177227	MS.gene91613:CDS	30.0%
	AAACGATGTTACCAGCTAAA+GGG	+	chr7.4:85176836-85176855	None:intergenic	35.0%
	AACAAGTCAAAGAAAGTGAG+AGG	+	chr7.4:85176678-85176697	None:intergenic	35.0%
	AATCTTTAAGCTTCTCACGT+TGG	+	chr7.4:85177318-85177337	None:intergenic	35.0%
	ACAAGTCAAAGAAAGTGAGA+GGG	+	chr7.4:85176677-85176696	None:intergenic	35.0%
	ATGATTGGTGAAGGAATTCA+AGG	-	chr7.4:85177797-85177816	MS.gene91613:CDS	35.0%
	TATTTGGCTATGATTGGTGA+AGG	-	chr7.4:85177788-85177807	MS.gene91613:CDS	35.0%
	TGTTTCCGTTTGTATAGCAA+AGG	-	chr7.4:85177514-85177533	MS.gene91613:CDS	35.0%
!	AAGGGTGTGATTGTGAATTT+GGG	+	chr7.4:85176746-85176765	None:intergenic	35.0%
!	CCAATGGAAAAACCAAGTTT+TGG	+	chr7.4:85177074-85177093	None:intergenic	35.0%
!	TATCGCCTTTGCTATACAAA+CGG	+	chr7.4:85177522-85177541	None:intergenic	35.0%
!	TTGAAAGGTGGTGTTGAATT+TGG	-	chr7.4:85177947-85177966	MS.gene91613:CDS	35.0%
!!	ATTGTTCGTGGATTTTGATG+TGG	+	chr7.4:85176617-85176636	None:intergenic	35.0%
!!	CCAAAACTTGGTTTTTCCAT+TGG	-	chr7.4:85177071-85177090	MS.gene91613:CDS	35.0%
!!	GAAGTAATCTCCACTTTTGA+AGG	+	chr7.4:85177884-85177903	None:intergenic	35.0%
!!	GAATCTGTTGATTCTAAGTG+AGG	+	chr7.4:85177014-85177033	None:intergenic	35.0%
!!	GAGAGTGAAGATTTGAGTTT+TGG	+	chr7.4:85176792-85176811	None:intergenic	35.0%
!!	TGGATTTTGATGTGGTTGTT+TGG	+	chr7.4:85176609-85176628	None:intergenic	35.0%
!!	TTTAGAACCCTTTTTGGAGA+GGG	-	chr7.4:85177214-85177233	MS.gene91613:CDS	35.0%
!!!	AATGGTTTTGGAGAATCAGA+AGG	+	chr7.4:85176861-85176880	None:intergenic	35.0%
!!!	TCTTTGACTTGTTTTGGCTA+AGG	-	chr7.4:85176685-85176704	MS.gene91613:CDS	35.0%
!!!	TTTTAGAACCCTTTTTGGAG+AGG	-	chr7.4:85177213-85177232	MS.gene91613:CDS	35.0%
	AAGGAAACTCCGTCATCATT+TGG	+	chr7.4:85176897-85176916	None:intergenic	40.0%
	AAGTGAGAGGGAGAGATAAT+TGG	+	chr7.4:85176665-85176684	None:intergenic	40.0%
	AATCTTGATCTGCAATCCAC+AGG	+	chr7.4:85177590-85177609	None:intergenic	40.0%
	AGAAGTAGATACGTTCACCT+GGG	+	chr7.4:85176716-85176735	None:intergenic	40.0%
	ATGGTGTGTGCTTATACCAA+TGG	+	chr7.4:85177090-85177109	None:intergenic	40.0%
	ATTCAAGGAATAGGAGTTGC+AGG	-	chr7.4:85177812-85177831	MS.gene91613:CDS	40.0%
	CAAACGATGTTACCAGCTAA+AGG	+	chr7.4:85176837-85176856	None:intergenic	40.0%
	CAACCATGTTCATCAAAGCT+TGG	-	chr7.4:85177132-85177151	MS.gene91613:CDS	40.0%
	CCAGTTCCTGAAAACTACTT+TGG	-	chr7.4:85177617-85177636	MS.gene91613:CDS	40.0%
	CTTTAGCTGGTAACATCGTT+TGG	-	chr7.4:85176835-85176854	MS.gene91613:CDS	40.0%
	GGTGAAGGAATTCAAGGAAT+AGG	-	chr7.4:85177803-85177822	MS.gene91613:CDS	40.0%
	GTGCTCCAAAACTTTATCGA+AGG	+	chr7.4:85176952-85176971	None:intergenic	40.0%
	GTGTGATTGTGAATTTGGGA+GGG	+	chr7.4:85176742-85176761	None:intergenic	40.0%
	TAGAAGTAGATACGTTCACC+TGG	+	chr7.4:85176717-85176736	None:intergenic	40.0%
	TGTTCTTGAGAAGCAAGTGA+TGG	-	chr7.4:85177973-85177992	MS.gene91613:CDS	40.0%
	TTCAAGGAATAGGAGTTGCA+GGG	-	chr7.4:85177813-85177832	MS.gene91613:CDS	40.0%
!	AGACCAAGCTTTGATGAACA+TGG	+	chr7.4:85177138-85177157	None:intergenic	40.0%
!	ATTTTCCGTCAAGAACAGCA+TGG	+	chr7.4:85177109-85177128	None:intergenic	40.0%
!	CGAACGGAATCCTTAACTTT+TGG	+	chr7.4:85177359-85177378	None:intergenic	40.0%
!	GAAGGGTGTGATTGTGAATT+TGG	+	chr7.4:85176747-85176766	None:intergenic	40.0%
!!	AAGTAGTTTTCAGGAACTGG+TGG	+	chr7.4:85177617-85177636	None:intergenic	40.0%
!!	ATGGATAAGGGTGTTCTTGA+TGG	-	chr7.4:85177743-85177762	MS.gene91613:CDS	40.0%
!!	CCAAAGTAGTTTTCAGGAAC+TGG	+	chr7.4:85177620-85177639	None:intergenic	40.0%
!!	TGGATAAGGGTGTTCTTGAT+GGG	-	chr7.4:85177744-85177763	MS.gene91613:CDS	40.0%
!!	TTAGAACCCTTTTTGGAGAG+GGG	-	chr7.4:85177215-85177234	MS.gene91613:CDS	40.0%
!!!	TAAAGGGAGGAAGTTTTGGA+GGG	+	chr7.4:85176820-85176839	None:intergenic	40.0%
	ACACACCATGCTGTTCTTGA+CGG	-	chr7.4:85177101-85177120	MS.gene91613:CDS	45.0%
	ACTACTCCCCTCTCCAAAAA+GGG	+	chr7.4:85177224-85177243	None:intergenic	45.0%
	AGTTAAGGATTCCGTTCGAG+TGG	-	chr7.4:85177361-85177380	MS.gene91613:CDS	45.0%
	CAAAACTTCCTCCCTTTAGC+TGG	-	chr7.4:85176822-85176841	MS.gene91613:CDS	45.0%
	CGAGACAAAGAAGCTTCTTG+TGG	+	chr7.4:85176981-85177000	None:intergenic	45.0%
	GAGACACTTTGCATTGTTCG+TGG	+	chr7.4:85176629-85176648	None:intergenic	45.0%
	GGTGTGATTGTGAATTTGGG+AGG	+	chr7.4:85176743-85176762	None:intergenic	45.0%
	TACTACTCCCCTCTCCAAAA+AGG	+	chr7.4:85177225-85177244	None:intergenic	45.0%
	TTTCGGAAGGCCTTCAAAAG+TGG	-	chr7.4:85177871-85177890	MS.gene91613:CDS	45.0%
	TTTGGAGAGGGGAGTAGTAA+AGG	-	chr7.4:85177226-85177245	MS.gene91613:CDS	45.0%
!	CAGCTAAAGGGAGGAAGTTT+TGG	+	chr7.4:85176824-85176843	None:intergenic	45.0%
!!	CTAAAGGGAGGAAGTTTTGG+AGG	+	chr7.4:85176821-85176840	None:intergenic	45.0%
!!	TTTTGGCTAAGGTTTCACCC+AGG	-	chr7.4:85176696-85176715	MS.gene91613:CDS	45.0%
	AGTTGCAGGGTCAACAAGGT+TGG	-	chr7.4:85177826-85177845	MS.gene91613:CDS	50.0%
	CGATGTTACCAGCTAAAGGG+AGG	+	chr7.4:85176833-85176852	None:intergenic	50.0%
	GAGCGTGAATACCACTCGAA+CGG	+	chr7.4:85177375-85177394	None:intergenic	50.0%
	GTTGCAGGGTCAACAAGGTT+GGG	-	chr7.4:85177827-85177846	MS.gene91613:CDS	50.0%
	TAGGAGTTGCAGGGTCAACA+AGG	-	chr7.4:85177822-85177841	MS.gene91613:CDS	50.0%
	GTGGTATTCACGCTCACACG+CGG	-	chr7.4:85177380-85177399	MS.gene91613:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	85176597	85178039	85176597	ID=MS.gene91613
chr7.4	mRNA	85176597	85178039	85176597	ID=MS.gene91613.t1;Parent=MS.gene91613
chr7.4	exon	85176597	85178039	85176597	ID=MS.gene91613.t1.exon1;Parent=MS.gene91613.t1
chr7.4	CDS	85176597	85178039	85176597	ID=cds.MS.gene91613.t1;Parent=MS.gene91613.t1

Gene Sequence

>MS.gene91613

ATGGCTTCTCCAAACAACCACATCAAAATCCACGAACAATGCAAAGTGTCTCCTAATTCTGCAAACCAATTATCTCTCCCTCTCACTTTCTTTGACTTGTTTTGGCTAAGGTTTCACCCAGGTGAACGTATCTACTTCTACACCCTCCCAAATTCACAATCACACCCTTCTTTCTTTTTTGAAAACCTTGTTCCAAAACTCAAATCTTCACTCTCTTTAACCCTCCAAAACTTCCTCCCTTTAGCTGGTAACATCGTTTGGCCTTCTGATTCTCCAAAACCATTCATCAAATTCAATCCAAATGATGACGGAGTTTCCTTGCTCTTAACACAATGTAATGACGATGATGTTTCCTTCGATAAAGTTTTGGAGCACAATTCACCACAAGAAGCTTCTTTGTCTCGTTCTTTTGTACCTCACTTAGAATCAACAGATTCTTTTGCTTCTATTATTTCAATTCAGATAACCCTTTTTCCAAAACTTGGTTTTTCCATTGGTATAAGCACACACCATGCTGTTCTTGACGGAAAATCTTCAACCATGTTCATCAAAGCTTGGTCTTCTATATCTAAATCTTTTCAAGAAGAAGAAATGAAACCACCAAACTTAGATCCTTTTTTAGAACCCTTTTTGGAGAGGGGAGTAGTAAAGGATCCAAATAATTTTGAGAGTCTGTTCATAAATAATTGGGACAAAATCCTATCACATTTTCATAAGTCCAACGTGAGAAGCTTAAAGATTATGTCAGACTCATTTGAACCAAAAGTTAAGGATTCCGTTCGAGTGGTATTCACGCTCACACGCGGAGATTTAGAGAAATTAAACAAAAGGGTGTTGTCCAAATCGTACATTGTAAAAGGAGCACAAGAACATGAACAACCTAAGAAATTGTCAACTTTTGTTCTTACATGTGCTTATGTTTCCGTTTGTATAGCAAAGGCGATACAACAATCCGAAAGCGATAAAAAACAGAAATTTTCTTTTAGTTTTCCTGTGGATTGCAGATCAAGATTAGATCCACCAGTTCCTGAAAACTACTTTGGAAATTGTGTATGTAATCATATAGTTGATACTGAGTCAGATGATTTTACAAAGGAAGATGGTTTGGTAATTGTTGCTAATAAAATTTATGGCAAAACAAAAACAATGGATAAGGGTGTTCTTGATGGGATAGAGACATTGACGTCTAAATATTTGGCTATGATTGGTGAAGGAATTCAAGGAATAGGAGTTGCAGGGTCAACAAGGTTGGGAGTATATGAAATTGATTTTGGTTTCGGAAGGCCTTCAAAAGTGGAGATTACTTCAATTGATAGAGGATTAACTATTGGTTTAACAGAAAGTAATGATTTGAAAGGTGGTGTTGAATTTGGACTTGTTCTTGAGAAGCAAGTGATGGATTTGTTTCAAGCAATTTTTCGTGAAGGGTTGTATTTTGATTGA

Protein sequence

>MS.gene91613.t1

MASPNNHIKIHEQCKVSPNSANQLSLPLTFFDLFWLRFHPGERIYFYTLPNSQSHPSFFFENLVPKLKSSLSLTLQNFLPLAGNIVWPSDSPKPFIKFNPNDDGVSLLLTQCNDDDVSFDKVLEHNSPQEASLSRSFVPHLESTDSFASIISIQITLFPKLGFSIGISTHHAVLDGKSSTMFIKAWSSISKSFQEEEMKPPNLDPFLEPFLERGVVKDPNNFESLFINNWDKILSHFHKSNVRSLKIMSDSFEPKVKDSVRVVFTLTRGDLEKLNKRVLSKSYIVKGAQEHEQPKKLSTFVLTCAYVSVCIAKAIQQSESDKKQKFSFSFPVDCRSRLDPPVPENYFGNCVCNHIVDTESDDFTKEDGLVIVANKIYGKTKTMDKGVLDGIETLTSKYLAMIGEGIQGIGVAGSTRLGVYEIDFGFGRPSKVEITSIDRGLTIGLTESNDLKGGVEFGLVLEKQVMDLFQAIFREGLYFD