AlfalfaGEDB | MS.gene44633

M. sativa cultivar XinJiangDaYe / MS.gene44633

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016740 GO:0016746 GO:0016747
PFAM: PF02458.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44633.t1	RHN44407.1	92.1	480	38	0	1	480	1	480	7.80E-257	896

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44633.t1	Q940Z5	37.8	482	270	14	5	477	7	467	5.4e-81	303.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44633.t1	A0A396H0H8	92.1	480	38	0	1	480	1	480	5.6e-257	896.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049232	MS.gene44633	0.816052	6.75E-52	-1.69E-46
MS.gene050502	MS.gene44633	0.802514	5.41E-49	-1.69E-46
MS.gene051509	MS.gene44633	0.810526	1.10E-50	-1.69E-46
MS.gene052066	MS.gene44633	0.808856	2.52E-50	-1.69E-46
MS.gene052486	MS.gene44633	0.804213	2.41E-49	-1.69E-46
MS.gene053505	MS.gene44633	0.809031	2.31E-50	-1.69E-46
MS.gene053574	MS.gene44633	0.811413	7.09E-51	-1.69E-46
MS.gene053626	MS.gene44633	0.817158	3.81E-52	-1.69E-46
MS.gene053633	MS.gene44633	0.800541	1.37E-48	-1.69E-46
MS.gene056504	MS.gene44633	0.804655	1.95E-49	-1.69E-46
MS.gene057086	MS.gene44633	0.82205	2.91E-53	-1.69E-46
MS.gene05787	MS.gene44633	0.806536	7.84E-50	-1.69E-46
MS.gene059367	MS.gene44633	0.833893	4.10E-56	-1.69E-46
MS.gene059368	MS.gene44633	0.826686	2.37E-54	-1.69E-46
MS.gene059865	MS.gene44633	0.800807	1.21E-48	-1.69E-46
MS.gene060482	MS.gene44633	0.800876	1.17E-48	-1.69E-46
MS.gene060778	MS.gene44633	0.823306	1.49E-53	-1.69E-46
MS.gene06097	MS.gene44633	0.813757	2.18E-51	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene99221	MS.gene44633	PPI
MS.gene03202	MS.gene44633	PPI
MS.gene44633	MS.gene87577	PPI
MS.gene44633	MS.gene44643	PPI
MS.gene44633	MS.gene021905	PPI
MS.gene44633	MS.gene070270	PPI
MS.gene012360	MS.gene44633	PPI
MS.gene44642	MS.gene44633	PPI
MS.gene44633	MS.gene000764	PPI
MS.gene44633	MS.gene50382	PPI
MS.gene44633	MS.gene90496	PPI
MS.gene44633	MS.gene91614	PPI
MS.gene91609	MS.gene44633	PPI
MS.gene99222	MS.gene44633	PPI
MS.gene44633	MS.gene87191	PPI
MS.gene44633	MS.gene56796	PPI
MS.gene44633	MS.gene48584	PPI
MS.gene44633	MS.gene004218	PPI
MS.gene032349	MS.gene44633	PPI
MS.gene066656	MS.gene44633	PPI
MS.gene44633	MS.gene016477	PPI
MS.gene44633	MS.gene021913	PPI
MS.gene066660	MS.gene44633	PPI
MS.gene44633	MS.gene028994	PPI
MS.gene44633	MS.gene91596	PPI
MS.gene44633	MS.gene021914	PPI
MS.gene44633	MS.gene31540	PPI
MS.gene066657	MS.gene44633	PPI
MS.gene069423	MS.gene44633	PPI
MS.gene44633	MS.gene44640	PPI
MS.gene35744	MS.gene44633	PPI
MS.gene44643	MS.gene44633	PPI
MS.gene028999	MS.gene44633	PPI
MS.gene44633	MS.gene021904	PPI
MS.gene44633	MS.gene000763	PPI
MS.gene50379	MS.gene44633	PPI
MS.gene44633	MS.gene91613	PPI
MS.gene44633	MS.gene90492	PPI
MS.gene44633	MS.gene043350	PPI
MS.gene44633	MS.gene56798	PPI
MS.gene44633	MS.gene021906	PPI
MS.gene073299	MS.gene44633	PPI
MS.gene44633	MS.gene50384	PPI
MS.gene44633	MS.gene44642	PPI
MS.gene91597	MS.gene44633	PPI
MS.gene021916	MS.gene44633	PPI
MS.gene32261	MS.gene44633	PPI
MS.gene31546	MS.gene44633	PPI
MS.gene44633	MS.gene96337	PPI
MS.gene44633	MS.gene066658	PPI
MS.gene44633	MS.gene87496	PPI
MS.gene44633	MS.gene070019	PPI
MS.gene44633	MS.gene043348	PPI
MS.gene35741	MS.gene44633	PPI
MS.gene44633	MS.gene021917	PPI
MS.gene99219	MS.gene44633	PPI
MS.gene91598	MS.gene44633	PPI
MS.gene44633	MS.gene90493	PPI
MS.gene56795	MS.gene44633	PPI
MS.gene44633	MS.gene48581	PPI
MS.gene44633	MS.gene028998	PPI
MS.gene44633	MS.gene066657	PPI
MS.gene44633	MS.gene56799	PPI
MS.gene44633	MS.gene063865	PPI
MS.gene028996	MS.gene44633	PPI
MS.gene44633	MS.gene032349	PPI
MS.gene44633	MS.gene44630	PPI
MS.gene44633	MS.gene069422	PPI
MS.gene44633	MS.gene069767	PPI
MS.gene000764	MS.gene44633	PPI
MS.gene91615	MS.gene44633	PPI
MS.gene44633	MS.gene011716	PPI
MS.gene44633	MS.gene28251	PPI
MS.gene44633	MS.gene91595	PPI
MS.gene99223	MS.gene44633	PPI
MS.gene44633	MS.gene90491	PPI
MS.gene44633	MS.gene016532	PPI
MS.gene44633	MS.gene043352	PPI
MS.gene48585	MS.gene44633	PPI
MS.gene44633	MS.gene38977	PPI
MS.gene44633	MS.gene44647	PPI
MS.gene44633	MS.gene066701	PPI
MS.gene44633	MS.gene021909	PPI
MS.gene44633	MS.gene021915	PPI
MS.gene44633	MS.gene50355	PPI
MS.gene44633	MS.gene91612	PPI
MS.gene011837	MS.gene44633	PPI
MS.gene066634	MS.gene44633	PPI
MS.gene043346	MS.gene44633	PPI
MS.gene91599	MS.gene44633	PPI
MS.gene44633	MS.gene011799	PPI
MS.gene44633	MS.gene002544	PPI
MS.gene44633	MS.gene50420	PPI
MS.gene49853	MS.gene44633	PPI
MS.gene44633	MS.gene064101	PPI
MS.gene007831	MS.gene44633	PPI
MS.gene44633	MS.gene31545	PPI
MS.gene44633	MS.gene021910	PPI
MS.gene021910	MS.gene44633	PPI
MS.gene44633	MS.gene50356	PPI
MS.gene44633	MS.gene87536	PPI
MS.gene44633	MS.gene56797	PPI
MS.gene44644	MS.gene44633	PPI
MS.gene44633	MS.gene99218	PPI
MS.gene35745	MS.gene44633	PPI
MS.gene011796	MS.gene44633	PPI
MS.gene011801	MS.gene44633	PPI
MS.gene44633	MS.gene44463	PPI
MS.gene87537	MS.gene44633	PPI
MS.gene48582	MS.gene44633	PPI
MS.gene60208	MS.gene44633	PPI
MS.gene44633	MS.gene78818	PPI
MS.gene013787	MS.gene44633	PPI
MS.gene021906	MS.gene44633	PPI
MS.gene31542	MS.gene44633	PPI
MS.gene87532	MS.gene44633	PPI
MS.gene46265	MS.gene44633	PPI
MS.gene063868	MS.gene44633	PPI
MS.gene48581	MS.gene44633	PPI
MS.gene44633	MS.gene91611	PPI
MS.gene44633	MS.gene02573	PPI
MS.gene44633	MS.gene011798	PPI
MS.gene44633	MS.gene35740	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44633.t1	MTR_7g013850	78.468	483	101	2	1	480	1	483	0.0	779
MS.gene44633.t1	MTR_7g014200	60.041	483	172	11	1	477	1	468	0.0	555
MS.gene44633.t1	MTR_7g014160	58.333	480	185	10	6	480	7	476	0.0	547
MS.gene44633.t1	MTR_7g014610	59.588	485	180	12	1	478	1	476	0.0	526
MS.gene44633.t1	MTR_7g014620	52.688	465	182	9	19	480	6	435	1.23e-152	442
MS.gene44633.t1	MTR_7g014630	50.418	359	159	7	5	350	6	358	1.48e-108	327
MS.gene44633.t1	MTR_6g015320	39.501	481	260	10	1	477	1	454	1.81e-104	319
MS.gene44633.t1	MTR_6g015315	39.789	475	261	11	4	477	2	452	4.74e-104	318
MS.gene44633.t1	MTR_6g015850	40.369	488	249	14	1	477	1	457	1.09e-101	312
MS.gene44633.t1	MTR_6g015815	42.140	458	232	12	25	477	31	460	4.65e-101	311
MS.gene44633.t1	MTR_7g014330	39.545	483	249	16	9	477	3	456	6.88e-100	308
MS.gene44633.t1	MTR_7g014230	39.167	480	253	13	9	476	5	457	1.27e-99	307
MS.gene44633.t1	MTR_7g014250	38.831	479	258	13	9	477	3	456	1.68e-99	306
MS.gene44633.t1	MTR_6g015830	39.715	491	251	14	1	477	1	460	1.79e-99	306
MS.gene44633.t1	MTR_3g028210	35.052	485	272	16	8	477	8	464	3.31e-75	244
MS.gene44633.t1	MTR_7g014180	68.156	179	50	5	1	178	1	173	3.68e-73	229
MS.gene44633.t1	MTR_2g089765	32.114	492	279	14	11	477	21	482	1.06e-68	228
MS.gene44633.t1	MTR_7g014670	56.842	190	80	2	289	477	71	259	1.52e-68	220
MS.gene44633.t1	MTR_7g014360	34.550	411	215	13	76	477	21	386	3.41e-68	223
MS.gene44633.t1	MTR_7g015860	32.510	486	265	17	8	468	5	452	3.46e-60	204
MS.gene44633.t1	MTR_3g087640	30.957	491	289	16	9	477	10	472	1.92e-59	203
MS.gene44633.t1	MTR_2g089670	31.410	468	272	14	26	477	43	477	2.22e-59	202
MS.gene44633.t1	MTR_2g089650	29.485	485	297	12	8	477	9	463	1.93e-58	200
MS.gene44633.t1	MTR_2g089735	31.473	448	271	12	41	477	39	461	4.65e-56	193
MS.gene44633.t1	MTR_6g015310	32.840	405	199	12	51	455	2	333	4.48e-55	187
MS.gene44633.t1	MTR_2g089755	29.614	466	285	13	28	477	37	475	1.69e-54	190
MS.gene44633.t1	MTR_8g012750	30.655	473	279	16	26	475	24	470	3.21e-53	186
MS.gene44633.t1	MTR_7g014310	27.628	333	172	8	130	460	41	306	8.93e-35	132
MS.gene44633.t1	MTR_3g435300	31.624	234	135	6	7	229	3	222	7.43e-27	109
MS.gene44633.t1	MTR_2g089665	25.771	454	202	16	11	454	7	335	3.82e-26	109
MS.gene44633.t1	MTR_4g007540	25.682	440	253	20	8	429	3	386	1.15e-21	97.4
MS.gene44633.t1	MTR_3g070170	25.442	452	282	18	8	439	12	428	1.39e-21	97.8
MS.gene44633.t1	MTR_3g101900	24.558	452	281	16	28	466	30	434	9.88e-20	92.0
MS.gene44633.t1	MTR_6g045553	23.742	497	296	21	7	467	13	462	9.89e-20	92.0
MS.gene44633.t1	MTR_4g094838	27.088	443	231	18	19	439	19	391	1.65e-19	91.3
MS.gene44633.t1	MTR_8g075610	24.129	402	233	18	50	429	23	374	1.81e-19	90.9
MS.gene44633.t1	MTR_3g026950	24.256	437	266	19	64	474	51	448	1.61e-17	85.1
MS.gene44633.t1	MTR_2g064495	23.326	463	279	22	31	475	27	431	4.14e-17	84.0
MS.gene44633.t1	MTR_2g105330	23.326	463	279	22	31	475	27	431	6.75e-17	83.2
MS.gene44633.t1	MTR_7g014210	34.579	107	68	2	335	439	24	130	1.49e-15	74.7
MS.gene44633.t1	MTR_7g086000	24.682	393	222	14	67	429	60	408	2.26e-15	78.6
MS.gene44633.t1	MTR_3g026310	22.197	446	278	16	17	441	14	411	6.06e-15	77.4
MS.gene44633.t1	MTR_4g094842	24.755	408	220	16	62	439	57	407	7.72e-15	77.0
MS.gene44633.t1	MTR_8g096530	24.541	436	265	15	3	429	2	382	1.31e-14	76.3
MS.gene44633.t1	MTR_4g097090	24.350	423	256	17	26	428	28	406	1.98e-14	75.9
MS.gene44633.t1	MTR_3g101930	24.717	441	266	20	13	432	12	407	3.73e-14	74.7
MS.gene44633.t1	MTR_3g098980	25.694	288	170	11	67	351	66	312	3.86e-14	74.7
MS.gene44633.t1	MTR_7g014205	29.839	124	75	2	317	439	2	114	5.97e-14	68.9
MS.gene44633.t1	MTR_8g467580	23.973	438	241	16	17	429	15	385	8.21e-14	73.6
MS.gene44633.t1	MTR_7g014340	53.425	73	27	2	9	77	3	72	2.55e-13	66.2
MS.gene44633.t1	MTR_7g084940	23.177	384	226	14	61	429	63	392	2.68e-13	72.0
MS.gene44633.t1	MTR_8g467590	24.697	413	238	19	44	442	41	394	4.16e-13	71.6
MS.gene44633.t1	MTR_2g015430	20.350	457	296	13	8	456	2	398	1.15e-12	70.1
MS.gene44633.t1	MTR_4g127650	21.806	454	294	17	41	475	43	454	1.19e-12	70.1
MS.gene44633.t1	MTR_7g085990	23.975	317	206	12	36	349	30	314	2.75e-11	65.9
MS.gene44633.t1	MTR_8g079980	23.894	452	267	19	17	453	15	404	3.00e-11	65.9
MS.gene44633.t1	MTR_7g014220	58.491	53	19	1	25	77	15	64	4.76e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44633.t1	AT5G39050	37.759	482	270	14	5	477	7	467	2.14e-90	283
MS.gene44633.t1	AT3G29590	36.515	482	267	9	1	477	1	448	9.76e-90	281
MS.gene44633.t1	AT3G29635	38.074	457	253	12	24	477	25	454	4.57e-89	280
MS.gene44633.t1	AT5G39080	37.083	480	274	13	1	474	1	458	5.06e-87	275
MS.gene44633.t1	AT5G39090	36.250	480	270	12	1	477	1	447	1.07e-86	273
MS.gene44633.t1	AT3G29670	37.161	479	260	14	8	477	3	449	2.53e-85	270
MS.gene44633.t1	AT5G61160	33.404	473	283	13	8	475	3	448	7.85e-76	245
MS.gene44633.t1	AT3G29680	35.849	477	264	14	4	473	2	443	1.09e-74	242
MS.gene44633.t1	AT1G03940	29.800	500	292	13	1	477	1	464	2.60e-64	216
MS.gene44633.t1	AT1G03495	30.728	371	216	12	1	359	1	342	3.23e-42	153
MS.gene44633.t1	AT5G23940	25.546	458	265	21	8	439	3	410	2.80e-22	100
MS.gene44633.t1	AT2G19070	24.938	405	248	17	41	428	37	402	1.98e-19	90.9
MS.gene44633.t1	AT3G29720	34.247	219	103	10	99	303	10	201	5.09e-19	86.3
MS.gene44633.t1	AT3G50280	24.242	429	263	16	19	434	18	397	1.43e-18	88.6
MS.gene44633.t1	AT5G67150	24.299	428	275	11	16	434	13	400	1.72e-18	88.2
MS.gene44633.t1	AT3G29690	31.126	151	98	2	328	477	33	178	3.56e-16	77.0
MS.gene44633.t1	AT1G31490	25.747	435	256	20	25	449	26	403	9.65e-15	76.6
MS.gene44633.t1	AT5G07850	24.832	447	282	19	1	434	1	406	1.56e-14	76.3
MS.gene44633.t1	AT2G39980	23.663	486	333	18	1	467	1	467	2.24e-13	72.4
MS.gene44633.t1	AT4G31910	21.910	356	235	8	44	389	46	368	2.90e-12	68.9
MS.gene44633.t1	AT3G47170	24.294	354	223	13	45	388	51	369	5.25e-12	68.2
MS.gene44633.t1	AT5G41040	22.520	373	244	14	61	429	63	394	7.87e-11	64.3
MS.gene44633.t1	AT5G41040	22.520	373	244	14	61	429	79	410	8.81e-11	64.3

Find 73 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TACATTGTTGTCTAAATATT+TGG	0.130040	7.3:-83581087	MS.gene44633:CDS
GATTACTTCCCTACTCAAAA+AGG	0.196644	7.3:+83581640	None:intergenic
GTAGGGTGTGATTGTGAATT+TGG	0.209360	7.3:+83582115	None:intergenic
TTGAAAGGTGGTGTTGAATT+TGG	0.214452	7.3:-83580912	MS.gene44633:CDS
GAGAGTGAAGATTTGAGTTT+TGG	0.216722	7.3:+83582070	None:intergenic
GATAATTGGTTTGCAGAATT+AGG	0.230849	7.3:+83582211	None:intergenic
ACAATTTCCGAAATAGATTT+CGG	0.252538	7.3:+83581235	None:intergenic
ACAAAAGTTGACAATTTCTT+TGG	0.263553	7.3:+83581383	None:intergenic
AACGAAACTCCGTCATCTTT+TGG	0.264453	7.3:+83581965	None:intergenic
GGAGTATATGAGATTGATTT+TGG	0.273264	7.3:-83581011	MS.gene44633:CDS
AAGTGAGAGGAAGAGATAAT+TGG	0.284573	7.3:+83582197	None:intergenic
CGAACGGAATCCTTAACTTT+TGG	0.284718	7.3:+83581506	None:intergenic
TTCTTCAATATTGTTCCATT+TGG	0.285958	7.3:+83581427	None:intergenic
CAATTTCCGAAATAGATTTC+GGG	0.286056	7.3:+83581236	None:intergenic
CCGAAATAGATTTCGGGAAT+TGG	0.301386	7.3:+83581242	None:intergenic
TCTTCCTTGGTAAAGTCTTC+TGG	0.306022	7.3:+83581185	None:intergenic
TTGAACTGGATGATTGGTTT+TGG	0.318638	7.3:+83581989	None:intergenic
TGAAAGGTGGTGTTGAATTT+GGG	0.328871	7.3:-83580911	MS.gene44633:CDS
CTATAGCCGGTAACATTGTT+TGG	0.330391	7.3:-83582024	MS.gene44633:CDS
TGAGCTCACACGTGAAGATT+TGG	0.334070	7.3:-83581474	MS.gene44633:CDS
CCAATTCCCGAAATCTATTT+CGG	0.335034	7.3:-83581242	MS.gene44633:CDS
TCACTTTCTTAGACTTGAAT+TGG	0.344769	7.3:-83582180	MS.gene44633:CDS
GTTGATAGAGGAATAACAAT+TGG	0.386783	7.3:-83580954	MS.gene44633:CDS
TCTAAATATTTGGCTATGAT+TGG	0.398839	7.3:-83581077	MS.gene44633:CDS
ATCTCACTCTCAAAACCATT+TGG	0.401328	7.3:+83581611	None:intergenic
TAGAAGAAAATACGTTCAAC+TGG	0.403167	7.3:+83582145	None:intergenic
ACACATCATGCTGTTCTTGA+TGG	0.407163	7.3:-83581758	MS.gene44633:CDS
ATTCAAGGAATAGGAGTTGC+AGG	0.408252	7.3:-83581047	MS.gene44633:CDS
CAAACAATGTTACCGGCTAT+AGG	0.417276	7.3:+83582025	None:intergenic
AAACAATGTTACCGGCTATA+GGG	0.421018	7.3:+83582026	None:intergenic
TTTGGATTGAACTGGATGAT+TGG	0.424802	7.3:+83581983	None:intergenic
GGTGAAGGAATTCAAGGAAT+AGG	0.425888	7.3:-83581056	MS.gene44633:CDS
AGAAGGCCAAACAATGTTAC+CGG	0.429788	7.3:+83582018	None:intergenic
ACAAAAGTGTGTTGTCCAAA+TGG	0.432381	7.3:-83581442	MS.gene44633:CDS
TAGGGTGTGATTGTGAATTT+GGG	0.451765	7.3:+83582116	None:intergenic
TTTGAGTAGGGAAGTAATCA+AGG	0.453618	7.3:-83581636	MS.gene44633:CDS
GTTGCAGGGTCAACAAGGTT+TGG	0.454958	7.3:-83581032	MS.gene44633:CDS
AGAGTGAGATCATAAATAGT+TGG	0.463701	7.3:-83581598	MS.gene44633:CDS
CGGCTATAGGGAGAAAGTAT+TGG	0.472914	7.3:+83582038	None:intergenic
AATCACCACTCCATCTTCCT+TGG	0.480258	7.3:+83581172	None:intergenic
GTGTGATTGTGAATTTGGGA+GGG	0.498440	7.3:+83582120	None:intergenic
ATGATTGGTGAAGGAATTCA+AGG	0.503765	7.3:-83581062	MS.gene44633:CDS
GTTCTTGATAAGCACGTGAT+GGG	0.509399	7.3:-83580885	MS.gene44633:CDS
TGTTCTTGATAAGCACGTGA+TGG	0.510983	7.3:-83580886	MS.gene44633:CDS
GTGATTGTGAATTTGGGAGG+GGG	0.512685	7.3:+83582122	None:intergenic
GAAAGTAGAGATTTGAAAGG+TGG	0.519737	7.3:-83580924	MS.gene44633:CDS
AAATGGAACAATATTGAAGA+AGG	0.523989	7.3:-83581425	MS.gene44633:CDS
TTATGGAAGGCCTGCAAAAG+TGG	0.524298	7.3:-83580988	MS.gene44633:CDS
TGTGATTGTGAATTTGGGAG+GGG	0.526202	7.3:+83582121	None:intergenic
GAAGTAATCAAGGATCCAAA+TGG	0.526584	7.3:-83581626	MS.gene44633:CDS
AAATAGATTTCGGGAATTGG+TGG	0.534901	7.3:+83581245	None:intergenic
GAAGACTTTACCAAGGAAGA+TGG	0.540696	7.3:-83581182	MS.gene44633:CDS
GAGACAACTTCAGTTGATAG+AGG	0.566864	7.3:-83580966	MS.gene44633:CDS
AGTTTCCTTTGATAAAATTG+TGG	0.567981	7.3:-83581915	MS.gene44633:CDS
CAATACTTTCTCCCTATAGC+CGG	0.569370	7.3:-83582037	MS.gene44633:CDS
ACAGAAAGTAGAGATTTGAA+AGG	0.576880	7.3:-83580927	MS.gene44633:CDS
TATTTGGCTATGATTGGTGA+AGG	0.577286	7.3:-83581071	MS.gene44633:CDS
GAGCTCAAATGCGACTCGAA+CGG	0.577817	7.3:+83581490	None:intergenic
CTATAGGGAGAAAGTATTGG+AGG	0.584990	7.3:+83582041	None:intergenic
GGTGTGATTGTGAATTTGGG+AGG	0.587405	7.3:+83582119	None:intergenic
TTCAAGTCTAAGAAAGTGAG+AGG	0.591765	7.3:+83582184	None:intergenic
GAATCAGTTGATTCTAAGTG+AGG	0.601514	7.3:+83581848	None:intergenic
TAGGAGTTGCAGGGTCAACA+AGG	0.619039	7.3:-83581037	MS.gene44633:CDS
CGAGATAAAGAAGCTTCTTG+TGG	0.621141	7.3:+83581881	None:intergenic
CAGTTCAATCCAAAAGATGA+CGG	0.625889	7.3:-83581974	MS.gene44633:CDS
CAACCATGTTCATCAAAGCA+TGG	0.631200	7.3:-83581727	MS.gene44633:CDS
CTTTACCAAGGAAGATGGAG+TGG	0.638020	7.3:-83581177	MS.gene44633:CDS
TATAGGGAGAAAGTATTGGA+GGG	0.638240	7.3:+83582042	None:intergenic
ATGATGTGTGCTTATTCCAA+TGG	0.647501	7.3:+83581772	None:intergenic
AGACCATGCTTTGATGAACA+TGG	0.653845	7.3:+83581724	None:intergenic
TTCAAGGAATAGGAGTTGCA+GGG	0.658447	7.3:-83581046	MS.gene44633:CDS
AGAAGAAAATACGTTCAACT+GGG	0.676059	7.3:+83582146	None:intergenic
TGAGCCAGAAGACTTTACCA+AGG	0.738299	7.3:-83581189	MS.gene44633:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATTAGAAAAATGGATAA+GGG	-	chr7.3:83581976-83581995	MS.gene44633:CDS	15.0%
!!	TTATAGCAAAATTAGAAAAA+TGG	-	chr7.3:83581969-83581988	MS.gene44633:CDS	15.0%
!!!	CAAAAACAAAAATTTTGTTT+TGG	-	chr7.3:83581805-83581824	MS.gene44633:CDS	15.0%
!!	CAAAATTAGAAAAATGGATA+AGG	-	chr7.3:83581975-83581994	MS.gene44633:CDS	20.0%
!!	TACATTGTTGTCTAAATATT+TGG	-	chr7.3:83582017-83582036	MS.gene44633:CDS	20.0%
!!!	TTTTTTTGAACCAAAAGTTA+AGG	-	chr7.3:83581588-83581607	MS.gene44633:CDS	20.0%
!	AAATGGAACAATATTGAAGA+AGG	-	chr7.3:83581679-83581698	MS.gene44633:CDS	25.0%
!	ACAAAAGTTGACAATTTCTT+TGG	+	chr7.3:83581724-83581743	None:intergenic	25.0%
!	ACAATTTCCGAAATAGATTT+CGG	+	chr7.3:83581872-83581891	None:intergenic	25.0%
!	AGTTTCCTTTGATAAAATTG+TGG	-	chr7.3:83581189-83581208	MS.gene44633:CDS	25.0%
!	TCTAAATATTTGGCTATGAT+TGG	-	chr7.3:83582027-83582046	MS.gene44633:CDS	25.0%
!	TTCTTCAATATTGTTCCATT+TGG	+	chr7.3:83581680-83581699	None:intergenic	25.0%
!!!	TATGAGATTGATTTTGGTTA+TGG	-	chr7.3:83582099-83582118	MS.gene44633:CDS	25.0%
!!!	TTCATACCATTTTTCATGAA+GGG	-	chr7.3:83582247-83582266	MS.gene44633:CDS	25.0%
!!!	TTTCATACCATTTTTCATGA+AGG	-	chr7.3:83582246-83582265	MS.gene44633:CDS	25.0%
	ACAGAAAGTAGAGATTTGAA+AGG	-	chr7.3:83582177-83582196	MS.gene44633:CDS	30.0%
	AGAAGAAAATACGTTCAACT+GGG	+	chr7.3:83580961-83580980	None:intergenic	30.0%
	AGAGTGAGATCATAAATAGT+TGG	-	chr7.3:83581506-83581525	MS.gene44633:CDS	30.0%
	ATTACTTCCCTACTCAAAAA+GGG	+	chr7.3:83581466-83581485	None:intergenic	30.0%
	CAATTTCCGAAATAGATTTC+GGG	+	chr7.3:83581871-83581890	None:intergenic	30.0%
	CTCAAAAAGGGTTTCAAAAA+AGG	+	chr7.3:83581454-83581473	None:intergenic	30.0%
	TAGAAGAAAATACGTTCAAC+TGG	+	chr7.3:83580962-83580981	None:intergenic	30.0%
	TCACTTTCTTAGACTTGAAT+TGG	-	chr7.3:83580924-83580943	MS.gene44633:CDS	30.0%
!	ATAACTCTTTTTCCGAAAAG+TGG	-	chr7.3:83581304-83581323	MS.gene44633:CDS	30.0%
!	GTTGATAGAGGAATAACAAT+TGG	-	chr7.3:83582150-83582169	MS.gene44633:CDS	30.0%
!!	GATAATTGGTTTGCAGAATT+AGG	+	chr7.3:83580896-83580915	None:intergenic	30.0%
!!	GGAGTATATGAGATTGATTT+TGG	-	chr7.3:83582093-83582112	MS.gene44633:CDS	30.0%
!!	TATTGCTTTTGCTATGCAAA+CGG	+	chr7.3:83581767-83581786	None:intergenic	30.0%
!!!	AAGATTTGAGTTTTGGAACA+AGG	+	chr7.3:83581030-83581049	None:intergenic	30.0%
!!!	AGATTGATTTTGGTTATGGA+AGG	-	chr7.3:83582103-83582122	MS.gene44633:CDS	30.0%
!!!	AGGTTTTGAAAGAAGAAAGT+AGG	+	chr7.3:83581010-83581029	None:intergenic	30.0%
!!!	ATTTTGTTTTGGTTTTGCAG+TGG	-	chr7.3:83581816-83581835	MS.gene44633:CDS	30.0%
!!!	GGTTTTGAAAGAAGAAAGTA+GGG	+	chr7.3:83581009-83581028	None:intergenic	30.0%
!!!	TTTGAAACCCTTTTTGAGTA+GGG	-	chr7.3:83581456-83581475	MS.gene44633:CDS	30.0%
!!!	TTTTGAAACCCTTTTTGAGT+AGG	-	chr7.3:83581455-83581474	MS.gene44633:CDS	30.0%
	AAATAGATTTCGGGAATTGG+TGG	+	chr7.3:83581862-83581881	None:intergenic	35.0%
	AAGTGAGAGGAAGAGATAAT+TGG	+	chr7.3:83580910-83580929	None:intergenic	35.0%
	ATCTCACTCTCAAAACCATT+TGG	+	chr7.3:83581496-83581515	None:intergenic	35.0%
	ATGATGTGTGCTTATTCCAA+TGG	+	chr7.3:83581335-83581354	None:intergenic	35.0%
	ATGATTGGTGAAGGAATTCA+AGG	-	chr7.3:83582042-83582061	MS.gene44633:CDS	35.0%
	CAGTTCAATCCAAAAGATGA+CGG	-	chr7.3:83581130-83581149	MS.gene44633:CDS	35.0%
	CCAATTCCCGAAATCTATTT+CGG	-	chr7.3:83581862-83581881	MS.gene44633:CDS	35.0%
	GAAAGTAGAGATTTGAAAGG+TGG	-	chr7.3:83582180-83582199	MS.gene44633:CDS	35.0%
	GAAGTAATCAAGGATCCAAA+TGG	-	chr7.3:83581478-83581497	MS.gene44633:CDS	35.0%
	GATTACTTCCCTACTCAAAA+AGG	+	chr7.3:83581467-83581486	None:intergenic	35.0%
	GCATAACCCTTCATGAAAAA+TGG	+	chr7.3:83582256-83582275	None:intergenic	35.0%
	TAGGGTGTGATTGTGAATTT+GGG	+	chr7.3:83580991-83581010	None:intergenic	35.0%
	TATTTGGCTATGATTGGTGA+AGG	-	chr7.3:83582033-83582052	MS.gene44633:CDS	35.0%
	TTCAAGTCTAAGAAAGTGAG+AGG	+	chr7.3:83580923-83580942	None:intergenic	35.0%
	TTTGAGTAGGGAAGTAATCA+AGG	-	chr7.3:83581468-83581487	MS.gene44633:CDS	35.0%
	TTTGGATTGAACTGGATGAT+TGG	+	chr7.3:83581124-83581143	None:intergenic	35.0%
!	AAACAATGTTACCGGCTATA+GGG	+	chr7.3:83581081-83581100	None:intergenic	35.0%
!	ACAAAAGTGTGTTGTCCAAA+TGG	-	chr7.3:83581662-83581681	MS.gene44633:CDS	35.0%
!	ATGGATAAGGGTTTTCTTGA+TGG	-	chr7.3:83581988-83582007	MS.gene44633:CDS	35.0%
!	CTGCTCCACAATTTTATCAA+AGG	+	chr7.3:83581197-83581216	None:intergenic	35.0%
!	GAATCAGTTGATTCTAAGTG+AGG	+	chr7.3:83581259-83581278	None:intergenic	35.0%
!	TGAAAGGTGGTGTTGAATTT+GGG	-	chr7.3:83582193-83582212	MS.gene44633:CDS	35.0%
!	TTGAAAGGTGGTGTTGAATT+TGG	-	chr7.3:83582192-83582211	MS.gene44633:CDS	35.0%
!!	CCAATGGAAAAACCACTTTT+CGG	+	chr7.3:83581319-83581338	None:intergenic	35.0%
!!	GAGAGTGAAGATTTGAGTTT+TGG	+	chr7.3:83581037-83581056	None:intergenic	35.0%
!!	TATAGGGAGAAAGTATTGGA+GGG	+	chr7.3:83581065-83581084	None:intergenic	35.0%
!!!	ATTGGTTTTGGAGAATCAGA+AGG	+	chr7.3:83581106-83581125	None:intergenic	35.0%
!!!	TTGAACTGGATGATTGGTTT+TGG	+	chr7.3:83581118-83581137	None:intergenic	35.0%
	ACACATCATGCTGTTCTTGA+TGG	-	chr7.3:83581346-83581365	MS.gene44633:CDS	40.0%
	AGAAGGCCAAACAATGTTAC+CGG	+	chr7.3:83581089-83581108	None:intergenic	40.0%
	AGACCATGCTTTGATGAACA+TGG	+	chr7.3:83581383-83581402	None:intergenic	40.0%
	ATTCAAGGAATAGGAGTTGC+AGG	-	chr7.3:83582057-83582076	MS.gene44633:CDS	40.0%
	CAACCATGTTCATCAAAGCA+TGG	-	chr7.3:83581377-83581396	MS.gene44633:CDS	40.0%
	CAATACTTTCTCCCTATAGC+CGG	-	chr7.3:83581067-83581086	MS.gene44633:CDS	40.0%
	CCGAAATAGATTTCGGGAAT+TGG	+	chr7.3:83581865-83581884	None:intergenic	40.0%
	CGAGATAAAGAAGCTTCTTG+TGG	+	chr7.3:83581226-83581245	None:intergenic	40.0%
	CTATAGCCGGTAACATTGTT+TGG	-	chr7.3:83581080-83581099	MS.gene44633:CDS	40.0%
	GAAGACTTTACCAAGGAAGA+TGG	-	chr7.3:83581922-83581941	MS.gene44633:CDS	40.0%
	GGTGAAGGAATTCAAGGAAT+AGG	-	chr7.3:83582048-83582067	MS.gene44633:CDS	40.0%
	GTAGGGTGTGATTGTGAATT+TGG	+	chr7.3:83580992-83581011	None:intergenic	40.0%
	GTGTGATTGTGAATTTGGGA+GGG	+	chr7.3:83580987-83581006	None:intergenic	40.0%
	GTTCTTGATAAGCACGTGAT+GGG	-	chr7.3:83582219-83582238	MS.gene44633:CDS	40.0%
	TCTTCCTTGGTAAAGTCTTC+TGG	+	chr7.3:83581922-83581941	None:intergenic	40.0%
	TGTGATTGTGAATTTGGGAG+GGG	+	chr7.3:83580986-83581005	None:intergenic	40.0%
	TGTTCTTGATAAGCACGTGA+TGG	-	chr7.3:83582218-83582237	MS.gene44633:CDS	40.0%
	TTCAAGGAATAGGAGTTGCA+GGG	-	chr7.3:83582058-83582077	MS.gene44633:CDS	40.0%
!	AACGAAACTCCGTCATCTTT+TGG	+	chr7.3:83581142-83581161	None:intergenic	40.0%
!	CAAACAATGTTACCGGCTAT+AGG	+	chr7.3:83581082-83581101	None:intergenic	40.0%
!	CCGAAAAGTGGTTTTTCCAT+TGG	-	chr7.3:83581316-83581335	MS.gene44633:CDS	40.0%
!	CGAACGGAATCCTTAACTTT+TGG	+	chr7.3:83581601-83581620	None:intergenic	40.0%
!	CGTCATCTTTTGGATTGAAC+TGG	+	chr7.3:83581132-83581151	None:intergenic	40.0%
!!	CTATAGGGAGAAAGTATTGG+AGG	+	chr7.3:83581066-83581085	None:intergenic	40.0%
!!	GAGACAACTTCAGTTGATAG+AGG	-	chr7.3:83582138-83582157	MS.gene44633:CDS	40.0%
	AATCACCACTCCATCTTCCT+TGG	+	chr7.3:83581935-83581954	None:intergenic	45.0%
	CTTTACCAAGGAAGATGGAG+TGG	-	chr7.3:83581927-83581946	MS.gene44633:CDS	45.0%
	GGTGTGATTGTGAATTTGGG+AGG	+	chr7.3:83580988-83581007	None:intergenic	45.0%
	GTGATTGTGAATTTGGGAGG+GGG	+	chr7.3:83580985-83581004	None:intergenic	45.0%
	TGAGCCAGAAGACTTTACCA+AGG	-	chr7.3:83581915-83581934	MS.gene44633:CDS	45.0%
	TGAGCTCACACGTGAAGATT+TGG	-	chr7.3:83581630-83581649	MS.gene44633:CDS	45.0%
	TTATGGAAGGCCTGCAAAAG+TGG	-	chr7.3:83582116-83582135	MS.gene44633:CDS	45.0%
!	CGGCTATAGGGAGAAAGTAT+TGG	+	chr7.3:83581069-83581088	None:intergenic	45.0%
!!	GAAGTTGTCTCCACTTTTGC+AGG	+	chr7.3:83582129-83582148	None:intergenic	45.0%
	GAGCTCAAATGCGACTCGAA+CGG	+	chr7.3:83581617-83581636	None:intergenic	50.0%
	GTTGCAGGGTCAACAAGGTT+TGG	-	chr7.3:83582072-83582091	MS.gene44633:CDS	50.0%
	TAGGAGTTGCAGGGTCAACA+AGG	-	chr7.3:83582067-83582086	MS.gene44633:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	83580842	83582284	83580842	ID=MS.gene44633
chr7.3	mRNA	83580842	83582284	83580842	ID=MS.gene44633.t1;Parent=MS.gene44633
chr7.3	exon	83580842	83582284	83580842	ID=MS.gene44633.t1.exon1;Parent=MS.gene44633.t1
chr7.3	CDS	83580842	83582284	83580842	ID=cds.MS.gene44633.t1;Parent=MS.gene44633.t1

Gene Sequence

>MS.gene44633

ATGGCTGCTGCAAAGAATCACATCAAAATTCATGAAGAATGTAAAGTTTCTCCTAATTCTGCAAACCAATTATCTCTTCCTCTCACTTTCTTAGACTTGAATTGGCTAAGATTTCACCCAGTTGAACGTATTTTCTTCTACCCCCTCCCAAATTCACAATCACACCCTACTTTCTTCTTTCAAAACCTTGTTCCAAAACTCAAATCTTCACTCTCTTTAACCCTCCAATACTTTCTCCCTATAGCCGGTAACATTGTTTGGCCTTCTGATTCTCCAAAACCAATCATCCAGTTCAATCCAAAAGATGACGGAGTTTCGTTGCTCTTAGCGCAATATGATGACGATGTAGTTTCCTTTGATAAAATTGTGGAGCAGAATTCACCACAAGAAGCTTCTTTATCTCGTTCTTTTGTACCTCACTTAGAATCAACTGATTCTTTTGCTTCTATCATTTCAATTCAGATAACTCTTTTTCCGAAAAGTGGTTTTTCCATTGGAATAAGCACACATCATGCTGTTCTTGATGGAAAATCTTCAACCATGTTCATCAAAGCATGGTCTTCTATATGTAAATCTCTTGAAGAAGAAACACAATCATCAAATTTAGAGCCTTTTTTGAAACCCTTTTTGAGTAGGGAAGTAATCAAGGATCCAAATGGTTTTGAGAGTGAGATCATAAATAGTTGGAACAAAATCTCAACACATTTTGATCAATGCAACGTGAGAAGCTTAAAGATTATGTCAAATTTTTTTGAACCAAAAGTTAAGGATTCCGTTCGAGTCGCATTTGAGCTCACACGTGAAGATTTGGATAAATTAAACAAAAGTGTGTTGTCCAAATGGAACAATATTGAAGAAGGATCACAAGAACAAGAACAACCAAAGAAATTGTCAACTTTTGTTCTTACATGTGCTTATGTTTCCGTTTGCATAGCAAAAGCAATACAACAATCTGAAAGCAATCAAAAACAAAAATTTTGTTTTGGTTTTGCAGTGGATATCAGATCAAGATTAGATCCACCAATTCCCGAAATCTATTTCGGAAATTGTGTAACTAATCATATAATTGATACTGAGCCAGAAGACTTTACCAAGGAAGATGGAGTGGTGATTGTTGCAAAGAAAATTTATAGCAAAATTAGAAAAATGGATAAGGGTTTTCTTGATGGTGTAGATACATTGTTGTCTAAATATTTGGCTATGATTGGTGAAGGAATTCAAGGAATAGGAGTTGCAGGGTCAACAAGGTTTGGAGTATATGAGATTGATTTTGGTTATGGAAGGCCTGCAAAAGTGGAGACAACTTCAGTTGATAGAGGAATAACAATTGGATTAACAGAAAGTAGAGATTTGAAAGGTGGTGTTGAATTTGGGCTTGTTCTTGATAAGCACGTGATGGGTTTTTTTCATACCATTTTTCATGAAGGGTTATGCTTTGATTGA

Protein sequence

>MS.gene44633.t1

MAAAKNHIKIHEECKVSPNSANQLSLPLTFLDLNWLRFHPVERIFFYPLPNSQSHPTFFFQNLVPKLKSSLSLTLQYFLPIAGNIVWPSDSPKPIIQFNPKDDGVSLLLAQYDDDVVSFDKIVEQNSPQEASLSRSFVPHLESTDSFASIISIQITLFPKSGFSIGISTHHAVLDGKSSTMFIKAWSSICKSLEEETQSSNLEPFLKPFLSREVIKDPNGFESEIINSWNKISTHFDQCNVRSLKIMSNFFEPKVKDSVRVAFELTREDLDKLNKSVLSKWNNIEEGSQEQEQPKKLSTFVLTCAYVSVCIAKAIQQSESNQKQKFCFGFAVDIRSRLDPPIPEIYFGNCVTNHIIDTEPEDFTKEDGVVIVAKKIYSKIRKMDKGFLDGVDTLLSKYLAMIGEGIQGIGVAGSTRFGVYEIDFGYGRPAKVETTSVDRGITIGLTESRDLKGGVEFGLVLDKHVMGFFHTIFHEGLCFD