AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44647

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44647.t1 MTR_7g014200 93.435 457 30 0 1 457 1 457 0.0 880
MS.gene44647.t1 MTR_7g014160 71.828 465 120 5 1 457 1 462 0.0 686
MS.gene44647.t1 MTR_7g014610 72.318 466 118 5 1 457 1 464 0.0 671
MS.gene44647.t1 MTR_7g014620 63.636 440 133 4 21 457 6 421 0.0 537
MS.gene44647.t1 MTR_7g013850 58.209 469 175 9 6 457 5 469 0.0 520
MS.gene44647.t1 MTR_7g014630 67.224 299 90 2 1 291 1 299 4.23e-140 406
MS.gene44647.t1 MTR_7g014230 42.763 456 240 11 7 454 2 444 1.39e-109 332
MS.gene44647.t1 MTR_7g014330 41.943 453 242 11 10 454 3 442 1.91e-108 328
MS.gene44647.t1 MTR_6g015320 42.384 453 238 11 7 454 6 440 5.17e-107 325
MS.gene44647.t1 MTR_6g015315 43.274 446 239 8 10 454 6 438 1.17e-104 319
MS.gene44647.t1 MTR_7g014250 41.280 453 245 11 10 454 3 442 5.76e-104 317
MS.gene44647.t1 MTR_6g015830 42.299 435 227 10 26 454 30 446 2.02e-102 313
MS.gene44647.t1 MTR_6g015815 41.215 461 246 12 3 454 2 446 4.05e-101 310
MS.gene44647.t1 MTR_6g015850 41.339 433 231 9 26 454 30 443 1.24e-100 309
MS.gene44647.t1 MTR_7g014670 74.419 172 43 1 286 457 78 248 1.15e-88 271
MS.gene44647.t1 MTR_7g014180 77.273 176 37 2 1 176 1 173 3.66e-87 264
MS.gene44647.t1 MTR_3g028210 35.512 459 267 12 9 454 8 450 4.14e-73 238
MS.gene44647.t1 MTR_7g014360 37.076 383 208 8 79 457 22 375 1.59e-71 231
MS.gene44647.t1 MTR_2g089765 32.857 490 268 16 3 454 2 468 7.21e-71 233
MS.gene44647.t1 MTR_2g089650 33.758 471 269 15 1 452 1 447 1.26e-64 216
MS.gene44647.t1 MTR_3g087640 34.061 458 262 15 17 452 17 456 4.04e-63 212
MS.gene44647.t1 MTR_2g089670 32.438 447 260 15 26 454 41 463 5.84e-62 209
MS.gene44647.t1 MTR_2g089735 35.129 427 240 16 43 452 39 445 1.18e-61 207
MS.gene44647.t1 MTR_2g089755 33.258 442 257 12 30 452 37 459 6.30e-61 206
MS.gene44647.t1 MTR_8g012750 32.026 459 260 16 26 454 22 458 2.24e-58 199
MS.gene44647.t1 MTR_7g015860 29.792 480 271 15 6 454 2 446 2.88e-56 193
MS.gene44647.t1 MTR_6g015310 32.412 398 199 10 53 446 2 333 7.22e-55 186
MS.gene44647.t1 MTR_7g014310 30.178 338 174 8 118 453 31 308 5.97e-38 140
MS.gene44647.t1 MTR_2g089665 27.333 450 194 14 9 442 2 334 8.54e-37 138
MS.gene44647.t1 MTR_3g435300 36.111 216 111 8 17 218 11 213 1.67e-32 125
MS.gene44647.t1 MTR_8g075610 25.648 386 235 17 46 420 30 374 1.19e-22 100
MS.gene44647.t1 MTR_5g084870 26.179 424 256 16 29 444 25 399 2.76e-22 99.4
MS.gene44647.t1 MTR_7g086000 24.324 444 269 14 8 421 3 409 3.33e-22 99.4
MS.gene44647.t1 MTR_4g007540 24.942 429 260 18 9 420 3 386 1.34e-21 97.1
MS.gene44647.t1 MTR_3g070170 25.519 482 293 22 2 452 5 451 1.42e-21 97.4
MS.gene44647.t1 MTR_6g045553 26.132 486 276 21 8 452 13 456 1.21e-20 94.7
MS.gene44647.t1 MTR_4g094838 27.212 452 243 18 20 449 18 405 6.59e-20 92.0
MS.gene44647.t1 MTR_4g094828 25.835 449 267 18 26 450 25 431 1.58e-19 91.3
MS.gene44647.t1 MTR_4g022825 24.249 433 285 12 28 450 22 421 4.30e-19 89.7
MS.gene44647.t1 MTR_4g022290 25.758 462 247 19 28 453 28 429 6.79e-19 89.0
MS.gene44647.t1 MTR_5g084850 25.527 427 254 18 29 444 25 398 7.62e-19 89.0
MS.gene44647.t1 MTR_8g079950 24.065 428 266 14 29 444 25 405 9.27e-19 89.0
MS.gene44647.t1 MTR_3g086020 25.935 401 225 17 64 452 56 396 1.37e-18 87.8
MS.gene44647.t1 MTR_3g088420 24.658 438 270 14 28 450 22 414 1.80e-18 87.8
MS.gene44647.t1 MTR_4g094835 27.753 454 241 22 28 450 30 427 2.61e-18 87.4
MS.gene44647.t1 MTR_8g096530 23.628 419 280 12 5 421 3 383 2.71e-18 87.4
MS.gene44647.t1 MTR_8g079970 23.474 426 271 15 29 444 25 405 3.57e-18 87.0
MS.gene44647.t1 MTR_8g079980 25.117 426 263 13 29 444 25 404 1.61e-17 84.7
MS.gene44647.t1 MTR_2g105330 23.832 428 265 19 9 420 3 385 1.79e-17 84.7
MS.gene44647.t1 MTR_7g085990 27.500 320 184 14 38 341 30 317 1.81e-17 85.1
MS.gene44647.t1 MTR_8g467580 23.188 414 259 14 21 420 17 385 2.13e-17 84.7
MS.gene44647.t1 MTR_2g064495 23.598 428 266 19 9 420 3 385 4.10e-17 83.6
MS.gene44647.t1 MTR_3g026310 24.541 436 288 14 28 450 22 429 4.46e-17 83.6
MS.gene44647.t1 MTR_2g015430 24.318 403 261 14 27 423 22 386 4.50e-17 83.6
MS.gene44647.t1 MTR_4g097090 23.356 441 281 16 1 419 1 406 5.39e-17 83.6
MS.gene44647.t1 MTR_7g085930 26.361 349 208 15 9 341 6 321 1.30e-16 82.4
MS.gene44647.t1 MTR_4g022800 26.770 452 268 18 19 450 15 423 4.52e-16 80.5
MS.gene44647.t1 MTR_3g049150 23.312 459 290 19 9 442 2 423 7.07e-15 77.0
MS.gene44647.t1 MTR_3g102010 24.648 426 267 19 30 443 30 413 7.83e-15 76.6
MS.gene44647.t1 MTR_7g084910 22.770 426 254 19 9 421 17 380 8.54e-15 76.6
MS.gene44647.t1 MTR_7g084940 23.419 427 264 16 9 421 16 393 9.13e-15 76.6
MS.gene44647.t1 MTR_4g022720 25.253 396 254 16 66 450 52 416 9.64e-15 76.3
MS.gene44647.t1 MTR_4g022670 25.618 445 281 17 19 450 15 422 1.24e-14 76.3
MS.gene44647.t1 MTR_6g053810 25.360 347 227 11 1 340 1 322 2.19e-14 75.5
MS.gene44647.t1 MTR_3g098980 25.191 393 247 16 69 452 66 420 2.87e-14 75.1
MS.gene44647.t1 MTR_7g014205 35.714 112 60 2 319 430 15 114 3.13e-14 69.3
MS.gene44647.t1 MTR_3g102020 25.310 403 259 18 33 424 32 403 4.03e-14 74.7
MS.gene44647.t1 MTR_4g094842 24.515 412 255 15 66 457 59 434 8.72e-14 73.6
MS.gene44647.t1 MTR_3g101960 22.693 401 267 14 30 419 29 397 1.00e-13 73.2
MS.gene44647.t1 MTR_4g094832 28.032 371 199 19 103 450 86 411 1.05e-13 73.2
MS.gene44647.t1 MTR_1g053315 22.739 387 252 14 46 420 58 409 1.10e-13 73.2
MS.gene44647.t1 MTR_2g007880 23.853 436 249 20 52 454 48 433 1.32e-13 72.8
MS.gene44647.t1 MTR_7g014340 50.704 71 29 2 10 77 3 70 6.26e-13 65.1
MS.gene44647.t1 MTR_7g014220 57.407 54 20 1 26 79 14 64 8.58e-13 64.3
MS.gene44647.t1 MTR_4g055170 24.194 372 244 14 52 417 59 398 1.76e-12 69.3
MS.gene44647.t1 MTR_7g027175 23.267 404 252 14 69 457 68 428 5.12e-12 67.8
MS.gene44647.t1 MTR_5g084900 22.135 384 229 12 65 444 5 322 1.02e-11 66.6
MS.gene44647.t1 MTR_3g465980 23.834 386 251 12 9 385 10 361 2.63e-11 65.9
MS.gene44647.t1 MTR_3g465910 24.107 336 221 8 8 338 9 315 5.53e-11 65.1
MS.gene44647.t1 MTR_3g465990 22.656 384 258 12 9 385 10 361 6.21e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44647.t1 AT3G29670 39.683 441 236 11 20 454 19 435 1.31e-94 293
MS.gene44647.t1 AT5G39050 40.000 460 250 14 7 454 8 453 1.22e-91 286
MS.gene44647.t1 AT3G29590 38.013 463 244 11 4 454 3 434 5.14e-91 283
MS.gene44647.t1 AT5G39080 38.964 444 244 14 22 454 20 447 4.82e-90 281
MS.gene44647.t1 AT5G39090 37.188 441 244 14 21 454 19 433 3.04e-85 269
MS.gene44647.t1 AT3G29635 36.175 434 250 10 28 454 27 440 4.43e-85 268
MS.gene44647.t1 AT5G61160 33.871 434 260 10 28 454 23 436 1.40e-75 244
MS.gene44647.t1 AT3G29680 34.382 445 240 11 28 454 23 433 8.03e-74 239
MS.gene44647.t1 AT1G03940 30.464 453 277 11 19 454 19 450 4.57e-61 206
MS.gene44647.t1 AT1G03495 31.642 335 197 10 19 340 19 334 1.74e-41 151
MS.gene44647.t1 AT5G23940 26.258 457 265 19 30 457 25 438 1.58e-26 112
MS.gene44647.t1 AT5G48930 26.512 430 252 20 9 420 3 386 1.88e-23 102
MS.gene44647.t1 AT3G29720 32.536 209 116 8 105 304 24 216 2.91e-22 95.1
MS.gene44647.t1 AT2G19070 23.624 436 271 17 9 419 4 402 7.45e-20 92.0
MS.gene44647.t1 AT5G67150 27.160 405 247 15 28 420 27 395 2.72e-19 90.5
MS.gene44647.t1 AT1G28680 24.033 362 234 11 69 423 62 389 5.71e-19 89.4
MS.gene44647.t1 AT1G28680 26.335 281 171 8 69 343 62 312 8.20e-17 82.4
MS.gene44647.t1 AT5G57840 24.516 465 295 20 9 457 3 427 1.22e-16 82.4
MS.gene44647.t1 AT3G29690 30.323 155 68 5 317 454 33 164 8.70e-16 75.9
MS.gene44647.t1 AT5G07850 23.947 451 287 16 16 445 9 424 2.44e-14 75.5
MS.gene44647.t1 AT5G67160 26.442 416 248 14 17 420 13 382 7.83e-14 73.6
MS.gene44647.t1 AT3G62160 23.684 418 254 18 18 423 11 375 2.01e-13 72.4
MS.gene44647.t1 AT5G63560 23.858 394 231 19 43 420 40 380 1.00e-12 70.1
MS.gene44647.t1 AT5G07860 24.714 437 276 18 33 455 34 431 1.36e-12 69.7
MS.gene44647.t1 AT2G23510 22.084 403 263 19 69 454 69 437 1.46e-12 69.7
MS.gene44647.t1 AT5G42830 25.879 398 232 21 64 443 59 411 7.53e-12 67.4
MS.gene44647.t1 AT5G07870 24.651 430 285 18 34 450 35 438 2.98e-11 65.9
MS.gene44647.t1 AT4G31910 23.077 403 233 15 12 389 24 374 3.70e-11 65.5

Find 90 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAAGTTCACGATCACTTTA+AGG 0.164541 7.3:-83365691 MS.gene44647:CDS
CCGATGCTAAAACCACTATT+TGG 0.173013 7.3:+83365249 None:intergenic
GAAGGGTGTGATTGTGAATT+TGG 0.182607 7.3:+83365564 None:intergenic
TCACTTTCTTCGACATATTT+TGG 0.186190 7.3:-83365629 MS.gene44647:CDS
CGAACAGAGTCTTCGAGTTT+TGG 0.241278 7.3:+83364982 None:intergenic
CTTTAGCTGGTAACATTGTT+TGG 0.264357 7.3:-83365473 MS.gene44647:CDS
AGTTCAAACCTTCATTCAAT+AGG 0.301095 7.3:-83365110 MS.gene44647:CDS
CAGTTTCCAAGATAGTTATT+TGG 0.312431 7.3:+83364724 None:intergenic
TAAGCTCAAACATGAAGATT+TGG 0.316500 7.3:-83364950 MS.gene44647:CDS
ACATTATTCCCAAGTGAAAA+AGG 0.317573 7.3:-83365039 MS.gene44647:CDS
ACTATCTTGGAAACTGTGTT+TGG 0.321806 7.3:-83364717 MS.gene44647:CDS
TATTTGGGAAAAGGGTGATT+TGG 0.325357 7.3:+83365265 None:intergenic
TCCATTACGTTATTTCCGTT+TGG 0.333263 7.3:+83365072 None:intergenic
AAGTGAAGGATTTGAAATAA+TGG 0.333426 7.3:-83364539 MS.gene44647:CDS
CAACCATGTTCGTCAATGCT+TGG 0.353190 7.3:-83365188 MS.gene44647:CDS
CCAAGATAGTTATTTGGTAT+TGG 0.355456 7.3:+83364730 None:intergenic
GGGAGAGGAGGGATAGAAGT+TGG 0.357090 7.3:-83364394 MS.gene44647:CDS
GGTGTTTATGAAATTGATTT+TGG 0.360240 7.3:-83364493 MS.gene44647:CDS
AACCATGTTCGTCAATGCTT+GGG 0.360973 7.3:-83365187 MS.gene44647:CDS
CCATTACGTTATTTCCGTTT+GGG 0.372843 7.3:+83365073 None:intergenic
ACTCATCATGCTGTTCTTGA+TGG 0.374958 7.3:-83365219 MS.gene44647:CDS
CGATGCTAAAACCACTATTT+GGG 0.375508 7.3:+83365250 None:intergenic
AACAAAGATGATAAATGAAA+AGG 0.386871 7.3:-83364614 MS.gene44647:CDS
GTTCAAACCTTCATTCAATA+GGG 0.388452 7.3:-83365109 MS.gene44647:CDS
AGTGAAGGATTTGAAATAAT+GGG 0.394576 7.3:-83364538 MS.gene44647:CDS
CCAATACCAAATAACTATCT+TGG 0.397200 7.3:-83364730 MS.gene44647:CDS
TTTAATGTTGCTGTTGTTGT+TGG 0.400453 7.3:+83365710 None:intergenic
TAGAAGAAAACGCGTTCTAC+TGG 0.401491 7.3:+83365594 None:intergenic
TAAAACCACTATTTGGGAAA+AGG 0.407089 7.3:+83365256 None:intergenic
AACGAGACAAAGAAGCTTCA+TGG 0.411299 7.3:+83365340 None:intergenic
GAGAGTGATGATTTGAGATT+CGG 0.414444 7.3:+83365519 None:intergenic
AAGGGTGTGATTGTGAATTT+GGG 0.425297 7.3:+83365565 None:intergenic
AATGAAACTCCACCGTTGTT+GGG 0.430287 7.3:+83365414 None:intergenic
TAGAGGTGTAACAATTGGTT+TGG 0.433565 7.3:-83364431 MS.gene44647:CDS
ATTGTTTGAAAGAAGAAAGA+AGG 0.435032 7.3:+83365546 None:intergenic
ACAAAGATGATAAATGAAAA+GGG 0.439203 7.3:-83364613 MS.gene44647:CDS
CTATCTTGGAAACTGTGTTT+GGG 0.444763 7.3:-83364716 MS.gene44647:CDS
ATAGATAGAGGTGTAACAAT+TGG 0.445177 7.3:-83364436 MS.gene44647:CDS
GAGATAGTGTCGATAGATAG+AGG 0.453075 7.3:-83364448 MS.gene44647:CDS
CAATGAAACTCCACCGTTGT+TGG 0.455744 7.3:+83365413 None:intergenic
GGTTTGGCAGAGAGCAAAGA+TGG 0.455777 7.3:-83364415 MS.gene44647:CDS
ATAAATGATGATAGAGTTTG+TGG 0.456698 7.3:+83364868 None:intergenic
ATGATTTCCCTATTGAATGA+AGG 0.463603 7.3:+83365102 None:intergenic
TGTGTTGCAAAAGCAATTCA+TGG 0.469489 7.3:-83364820 MS.gene44647:CDS
AAAACCACTATTTGGGAAAA+GGG 0.470209 7.3:+83365257 None:intergenic
GGAGAGGAGGGATAGAAGTT+GGG 0.470973 7.3:-83364393 MS.gene44647:CDS
AGATAGTTATTTGGTATTGG+TGG 0.479735 7.3:+83364733 None:intergenic
CAAACAATGTTACCAGCTAA+AGG 0.481339 7.3:+83365474 None:intergenic
GTGGCGGGGTCTCATAGATT+TGG 0.491756 7.3:-83364514 MS.gene44647:CDS
GAAGAAAGTGAGAGGGATTG+AGG 0.492974 7.3:+83365641 None:intergenic
TTTGAAATAATGGGAGTGGC+GGG 0.497904 7.3:-83364529 MS.gene44647:CDS
GAGAATCTGATGATTCTAAG+TGG 0.501577 7.3:+83365307 None:intergenic
AAACAATGTTACCAGCTAAA+GGG 0.504045 7.3:+83365475 None:intergenic
GTGTTGCAAAAGCAATTCAT+GGG 0.505428 7.3:-83364819 MS.gene44647:CDS
CTTATTTCTTTATCAAGTGA+AGG 0.507080 7.3:-83364553 MS.gene44647:CDS
CAGCTAAAGGGAGAAAATGT+TGG 0.510508 7.3:+83365487 None:intergenic
TTGGGATCAAATTGAATGAT+GGG 0.517571 7.3:+83365432 None:intergenic
GTTGGGATCAAATTGAATGA+TGG 0.517630 7.3:+83365431 None:intergenic
AACAAAGAGTGTTATCTACA+TGG 0.526232 7.3:-83364918 MS.gene44647:CDS
GACAAAGAAGCTTCATGGGG+TGG 0.529220 7.3:+83365345 None:intergenic
ACGAGACAAAGAAGCTTCAT+GGG 0.532033 7.3:+83365341 None:intergenic
ACAAAGAAGCTTCATGGGGT+GGG 0.543764 7.3:+83365346 None:intergenic
GAGAGCAAAGATGGGAGAGG+AGG 0.548569 7.3:-83364406 MS.gene44647:CDS
TTGTTTGAAAGAAGAAAGAA+GGG 0.551836 7.3:+83365547 None:intergenic
AGAATCTGATGATTCTAAGT+GGG 0.558214 7.3:+83365308 None:intergenic
AGAGCAAAGATGGGAGAGGA+GGG 0.573224 7.3:-83364405 MS.gene44647:CDS
AGAAAACGCGTTCTACTGGA+TGG 0.575757 7.3:+83365598 None:intergenic
CCCAAACGGAAATAACGTAA+TGG 0.576228 7.3:-83365073 MS.gene44647:CDS
TTTCGTTGATACACAGCCGT+TGG 0.584829 7.3:-83364689 MS.gene44647:CDS
CTAAAGGGAGAAAATGTTGG+AGG 0.587490 7.3:+83365490 None:intergenic
GAAATCATCAAAGACCCAAA+CGG 0.589530 7.3:-83365087 MS.gene44647:CDS
AGGATTTGAAATAATGGGAG+TGG 0.589904 7.3:-83364533 MS.gene44647:CDS
GCAGAGAGCAAAGATGGGAG+AGG 0.598380 7.3:-83364409 MS.gene44647:CDS
GTTTGGCAGAGAGCAAAGAT+GGG 0.598479 7.3:-83364414 MS.gene44647:CDS
GAAAGTGAGAGGGATTGAGG+TGG 0.600737 7.3:+83365644 None:intergenic
GTGTGATTGTGAATTTGGGA+GGG 0.604440 7.3:+83365569 None:intergenic
AGCCCAAGCATTGACGAACA+TGG 0.607159 7.3:+83365185 None:intergenic
ACAAAGAGTGTTATCTACAT+GGG 0.611657 7.3:-83364917 MS.gene44647:CDS
ATTTGAAATAATGGGAGTGG+CGG 0.616341 7.3:-83364530 MS.gene44647:CDS
GGTGTGATTGTGAATTTGGG+AGG 0.618307 7.3:+83365568 None:intergenic
TCAGCGTAGATTGCAGAGCA+AGG 0.630412 7.3:-83364762 MS.gene44647:CDS
AATATGTCGAAGAAAGTGAG+AGG 0.638159 7.3:+83365633 None:intergenic
CAATTTGATCCCAACAACGG+TGG 0.641969 7.3:-83365423 MS.gene44647:CDS
GAATCTGATGATTCTAAGTG+GGG 0.642142 7.3:+83365309 None:intergenic
TTGAAATAATGGGAGTGGCG+GGG 0.646292 7.3:-83364528 MS.gene44647:CDS
TGTTGCAAAAGCAATTCATG+GGG 0.654042 7.3:-83364818 MS.gene44647:CDS
TAAAGGGAGAAAATGTTGGA+GGG 0.659949 7.3:+83365491 None:intergenic
ATTCAATTTGATCCCAACAA+CGG 0.661555 7.3:-83365426 MS.gene44647:CDS
ATATGTCGAAGAAAGTGAGA+GGG 0.668848 7.3:+83365634 None:intergenic
CGAGACAAAGAAGCTTCATG+GGG 0.711268 7.3:+83365342 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATATTAAAATGAGTAA+TGG + chr7.3:83365306-83365325 None:intergenic 10.0%
!! AACAAAGATGATAAATGAAA+AGG - chr7.3:83364982-83365001 MS.gene44647:CDS 20.0%
!! ACAAAGATGATAAATGAAAA+GGG - chr7.3:83364983-83365002 MS.gene44647:CDS 20.0%
!!! TCTTCTTTTATAAAATCCAA+CGG + chr7.3:83364926-83364945 None:intergenic 20.0%
!!! TGAAATTAATTTTCTAGTTC+TGG - chr7.3:83365693-83365712 MS.gene44647:CDS 20.0%
!!! TGGTTCATTTTCAATATAAT+GGG - chr7.3:83365383-83365402 MS.gene44647:CDS 20.0%
!!! TTATGAAATTGATTTTGGTT+GGG - chr7.3:83365108-83365127 MS.gene44647:CDS 20.0%
!!! TTCAACAATTACTTTTTTAG+AGG - chr7.3:83365328-83365347 MS.gene44647:CDS 20.0%
!!! TTCTATAACTTATACTGATT+CGG - chr7.3:83365634-83365653 MS.gene44647:CDS 20.0%
!!! TTGGATTTTATAAAAGAAGA+TGG - chr7.3:83364926-83364945 MS.gene44647:CDS 20.0%
!!! TTTATGAAATTGATTTTGGT+TGG - chr7.3:83365107-83365126 MS.gene44647:CDS 20.0%
! ATAAATGATGATAGAGTTTG+TGG + chr7.3:83364731-83364750 None:intergenic 25.0%
! ATTGTTTGAAAGAAGAAAGA+AGG + chr7.3:83364053-83364072 None:intergenic 25.0%
! TGTTCTTAATAAACCTGTAA+TGG - chr7.3:83365228-83365247 MS.gene44647:CDS 25.0%
! TTGTTTGAAAGAAGAAAGAA+GGG + chr7.3:83364052-83364071 None:intergenic 25.0%
!! AAGTGAAGGATTTGAAATAA+TGG - chr7.3:83365057-83365076 MS.gene44647:CDS 25.0%
!! AGTGAAGGATTTGAAATAAT+GGG - chr7.3:83365058-83365077 MS.gene44647:CDS 25.0%
!! CTTATTTCTTTATCAAGTGA+AGG - chr7.3:83365043-83365062 MS.gene44647:CDS 25.0%
!! GTGGTTCATTTTCAATATAA+TGG - chr7.3:83365382-83365401 MS.gene44647:CDS 25.0%
!!! GGTGTTTATGAAATTGATTT+TGG - chr7.3:83365103-83365122 MS.gene44647:CDS 25.0%
!!! TATCATTTCCTTTTTCACTT+GGG + chr7.3:83364568-83364587 None:intergenic 25.0%
!!! TATGAAATTGATTTTGGTTG+GGG - chr7.3:83365109-83365128 MS.gene44647:CDS 25.0%
!!! TCTATAACTTATACTGATTC+GGG - chr7.3:83365635-83365654 MS.gene44647:CDS 25.0%
AAAACCACTATTTGGGAAAA+GGG + chr7.3:83364342-83364361 None:intergenic 30.0%
AAAATGAACCACACATAAAG+TGG + chr7.3:83365374-83365393 None:intergenic 30.0%
AAACAATGTTACCAGCTAAA+GGG + chr7.3:83364124-83364143 None:intergenic 30.0%
ACATTATTCCCAAGTGAAAA+AGG - chr7.3:83364557-83364576 MS.gene44647:CDS 30.0%
AGAATCTGATGATTCTAAGT+GGG + chr7.3:83364291-83364310 None:intergenic 30.0%
AGTTCAAACCTTCATTCAAT+AGG - chr7.3:83364486-83364505 MS.gene44647:CDS 30.0%
ATGATTTCCCTATTGAATGA+AGG + chr7.3:83364497-83364516 None:intergenic 30.0%
ATGGTTGAACTACTCATTAA+TGG + chr7.3:83365287-83365306 None:intergenic 30.0%
ATTCAATTTGATCCCAACAA+CGG - chr7.3:83364170-83364189 MS.gene44647:intron 30.0%
CAGTTTCCAAGATAGTTATT+TGG + chr7.3:83364875-83364894 None:intergenic 30.0%
CCAAGATAGTTATTTGGTAT+TGG + chr7.3:83364869-83364888 None:intergenic 30.0%
CCAATACCAAATAACTATCT+TGG - chr7.3:83364866-83364885 MS.gene44647:CDS 30.0%
CTATGCAAAAAAGCTATTGA+AGG - chr7.3:83364437-83364456 MS.gene44647:CDS 30.0%
GTTCAAACCTTCATTCAATA+GGG - chr7.3:83364487-83364506 MS.gene44647:CDS 30.0%
TAAAACCACTATTTGGGAAA+AGG + chr7.3:83364343-83364362 None:intergenic 30.0%
TAAAGTTCACGATCACTTTA+AGG - chr7.3:83363905-83363924 MS.gene44647:intron 30.0%
TAAGCTCAAACATGAAGATT+TGG - chr7.3:83364646-83364665 MS.gene44647:CDS 30.0%
TTACAATTCATGAGTACATG+AGG + chr7.3:83365481-83365500 None:intergenic 30.0%
TTGGGATCAAATTGAATGAT+GGG + chr7.3:83364167-83364186 None:intergenic 30.0%
! AATGAAAAGGGTTTTCTTGA+AGG - chr7.3:83364995-83365014 MS.gene44647:CDS 30.0%
! AGATAGTTATTTGGTATTGG+TGG + chr7.3:83364866-83364885 None:intergenic 30.0%
! ATAGATAGAGGTGTAACAAT+TGG - chr7.3:83365160-83365179 MS.gene44647:CDS 30.0%
! ATGAAAAGGGTTTTCTTGAA+GGG - chr7.3:83364996-83365015 MS.gene44647:CDS 30.0%
! TCACTTTCTTCGACATATTT+TGG - chr7.3:83363967-83363986 MS.gene44647:intron 30.0%
! TGATTCGGGAAATTAATTTC+TGG - chr7.3:83365649-83365668 MS.gene44647:CDS 30.0%
! TTTAATGTTGCTGTTGTTGT+TGG + chr7.3:83363889-83363908 None:intergenic 30.0%
! TTTAGCACTTTGTTTCTTGA+AGG - chr7.3:83365256-83365275 MS.gene44647:CDS 30.0%
! TTTTTCACTTGGGAATAATG+TGG + chr7.3:83364558-83364577 None:intergenic 30.0%
!! AACAAAGAGTGTTATCTACA+TGG - chr7.3:83364678-83364697 MS.gene44647:CDS 30.0%
!! ACAAAGAGTGTTATCTACAT+GGG - chr7.3:83364679-83364698 MS.gene44647:CDS 30.0%
!! ATTTCTGGTTGTGAATTTAC+TGG - chr7.3:83365664-83365683 MS.gene44647:CDS 30.0%
!! CTATCATTTCCTTTTTCACT+TGG + chr7.3:83364569-83364588 None:intergenic 30.0%
!!! ATGAAATTGATTTTGGTTGG+GGG - chr7.3:83365110-83365129 MS.gene44647:CDS 30.0%
AAGCAAGTGTAACTATGATG+AGG - chr7.3:83365502-83365521 MS.gene44647:CDS 35.0%
AATATGTCGAAGAAAGTGAG+AGG + chr7.3:83363966-83363985 None:intergenic 35.0%
ACTATCTTGGAAACTGTGTT+TGG - chr7.3:83364879-83364898 MS.gene44647:CDS 35.0%
ATATGTCGAAGAAAGTGAGA+GGG + chr7.3:83363965-83363984 None:intergenic 35.0%
CAAACAATGTTACCAGCTAA+AGG + chr7.3:83364125-83364144 None:intergenic 35.0%
CGATGCTAAAACCACTATTT+GGG + chr7.3:83364349-83364368 None:intergenic 35.0%
CTATCTTGGAAACTGTGTTT+GGG - chr7.3:83364880-83364899 MS.gene44647:CDS 35.0%
CTTTAGCTGGTAACATTGTT+TGG - chr7.3:83364123-83364142 MS.gene44647:intron 35.0%
GAAATCATCAAAGACCCAAA+CGG - chr7.3:83364509-83364528 MS.gene44647:CDS 35.0%
GAATCTGATGATTCTAAGTG+GGG + chr7.3:83364290-83364309 None:intergenic 35.0%
GAGAATCTGATGATTCTAAG+TGG + chr7.3:83364292-83364311 None:intergenic 35.0%
GTGCTAAAGATATCCATTAC+AGG + chr7.3:83365244-83365263 None:intergenic 35.0%
GTGTTGCAAAAGCAATTCAT+GGG - chr7.3:83364777-83364796 MS.gene44647:CDS 35.0%
GTTGGGATCAAATTGAATGA+TGG + chr7.3:83364168-83364187 None:intergenic 35.0%
TAAAGGGAGAAAATGTTGGA+GGG + chr7.3:83364108-83364127 None:intergenic 35.0%
TATTTGGGAAAAGGGTGATT+TGG + chr7.3:83364334-83364353 None:intergenic 35.0%
TGTGTTGCAAAAGCAATTCA+TGG - chr7.3:83364776-83364795 MS.gene44647:CDS 35.0%
TGTTGCAAAAGCAATTCATG+GGG - chr7.3:83364778-83364797 MS.gene44647:CDS 35.0%
! AAGGGTGTGATTGTGAATTT+GGG + chr7.3:83364034-83364053 None:intergenic 35.0%
! AGGATTTGAAATAATGGGAG+TGG - chr7.3:83365063-83365082 MS.gene44647:CDS 35.0%
! ATTTGAAATAATGGGAGTGG+CGG - chr7.3:83365066-83365085 MS.gene44647:CDS 35.0%
! CCATTACGTTATTTCCGTTT+GGG + chr7.3:83364526-83364545 None:intergenic 35.0%
! GAGAGTGATGATTTGAGATT+CGG + chr7.3:83364080-83364099 None:intergenic 35.0%
! TAGAGGTGTAACAATTGGTT+TGG - chr7.3:83365165-83365184 MS.gene44647:CDS 35.0%
! TCCATTACGTTATTTCCGTT+TGG + chr7.3:83364527-83364546 None:intergenic 35.0%
! TGAAAAGGGTTTTCTTGAAG+GGG - chr7.3:83364997-83365016 MS.gene44647:CDS 35.0%
!!! ATGGGTTTTGAAGAATCAGA+AGG + chr7.3:83364149-83364168 None:intergenic 35.0%
!!! CCAAATAGTGGTTTTAGCAT+CGG - chr7.3:83364347-83364366 MS.gene44647:intron 35.0%
!!! GTGGTTTTTGTTGATGATAG+AGG + chr7.3:83363936-83363955 None:intergenic 35.0%
!!! TCTTCGACATATTTTGGCTA+AGG - chr7.3:83363973-83363992 MS.gene44647:intron 35.0%
!!! TCTTCGAGTTTTGGTTCAAA+CGG + chr7.3:83364608-83364627 None:intergenic 35.0%
AACCATGTTCGTCAATGCTT+GGG - chr7.3:83364409-83364428 MS.gene44647:CDS 40.0%
AACGAGACAAAGAAGCTTCA+TGG + chr7.3:83364259-83364278 None:intergenic 40.0%
AATGAAACTCCACCGTTGTT+GGG + chr7.3:83364185-83364204 None:intergenic 40.0%
ACGAGACAAAGAAGCTTCAT+GGG + chr7.3:83364258-83364277 None:intergenic 40.0%
ACTCATCATGCTGTTCTTGA+TGG - chr7.3:83364377-83364396 MS.gene44647:CDS 40.0%
CAGCTAAAGGGAGAAAATGT+TGG + chr7.3:83364112-83364131 None:intergenic 40.0%
CCCAAACGGAAATAACGTAA+TGG - chr7.3:83364523-83364542 MS.gene44647:CDS 40.0%
CCGATGCTAAAACCACTATT+TGG + chr7.3:83364350-83364369 None:intergenic 40.0%
CTAAAGGGAGAAAATGTTGG+AGG + chr7.3:83364109-83364128 None:intergenic 40.0%
GTGTGATTGTGAATTTGGGA+GGG + chr7.3:83364030-83364049 None:intergenic 40.0%
TAGAAGAAAACGCGTTCTAC+TGG + chr7.3:83364005-83364024 None:intergenic 40.0%
! ATCACCCTTTTCCCAAATAG+TGG - chr7.3:83364335-83364354 MS.gene44647:intron 40.0%
! CAACATTTTCTCCCTTTAGC+TGG - chr7.3:83364110-83364129 MS.gene44647:intron 40.0%
! GAAGGGTGTGATTGTGAATT+TGG + chr7.3:83364035-83364054 None:intergenic 40.0%
! TTTGAAATAATGGGAGTGGC+GGG - chr7.3:83365067-83365086 MS.gene44647:CDS 40.0%
!! GAGATAGTGTCGATAGATAG+AGG - chr7.3:83365148-83365167 MS.gene44647:CDS 40.0%
!!! AAATTGATTTTGGTTGGGGG+AGG - chr7.3:83365113-83365132 MS.gene44647:CDS 40.0%
!!! CTTCGAGTTTTGGTTCAAAC+GGG + chr7.3:83364607-83364626 None:intergenic 40.0%
ACAAAGAAGCTTCATGGGGT+GGG + chr7.3:83364253-83364272 None:intergenic 45.0%
AGAAAACGCGTTCTACTGGA+TGG + chr7.3:83364001-83364020 None:intergenic 45.0%
CAACCATGTTCGTCAATGCT+TGG - chr7.3:83364408-83364427 MS.gene44647:CDS 45.0%
CAATGAAACTCCACCGTTGT+TGG + chr7.3:83364186-83364205 None:intergenic 45.0%
CAATTTGATCCCAACAACGG+TGG - chr7.3:83364173-83364192 MS.gene44647:intron 45.0%
CGAGACAAAGAAGCTTCATG+GGG + chr7.3:83364257-83364276 None:intergenic 45.0%
GAAGAAAGTGAGAGGGATTG+AGG + chr7.3:83363958-83363977 None:intergenic 45.0%
GGTGTGATTGTGAATTTGGG+AGG + chr7.3:83364031-83364050 None:intergenic 45.0%
GTTTGGCAGAGAGCAAAGAT+GGG - chr7.3:83365182-83365201 MS.gene44647:CDS 45.0%
TGAACCACACATAAAGTGGC+AGG + chr7.3:83365370-83365389 None:intergenic 45.0%
TTTCGTTGATACACAGCCGT+TGG - chr7.3:83364907-83364926 MS.gene44647:CDS 45.0%
! GACACTATCTCCACCTTTTC+CGG + chr7.3:83365139-83365158 None:intergenic 45.0%
! TTGAAATAATGGGAGTGGCG+GGG - chr7.3:83365068-83365087 MS.gene44647:CDS 45.0%
!! CGAACAGAGTCTTCGAGTTT+TGG + chr7.3:83364617-83364636 None:intergenic 45.0%
!! TGTTCCTGCCACTTTATGTG+TGG - chr7.3:83365363-83365382 MS.gene44647:CDS 45.0%
AGAGCAAAGATGGGAGAGGA+GGG - chr7.3:83365191-83365210 MS.gene44647:CDS 50.0%
GAAAGTGAGAGGGATTGAGG+TGG + chr7.3:83363955-83363974 None:intergenic 50.0%
GACAAAGAAGCTTCATGGGG+TGG + chr7.3:83364254-83364273 None:intergenic 50.0%
GGAGAGGAGGGATAGAAGTT+GGG - chr7.3:83365203-83365222 MS.gene44647:CDS 50.0%
GGTTTGGCAGAGAGCAAAGA+TGG - chr7.3:83365181-83365200 MS.gene44647:CDS 50.0%
TCAGCGTAGATTGCAGAGCA+AGG - chr7.3:83364834-83364853 MS.gene44647:CDS 50.0%
!! AGCCCAAGCATTGACGAACA+TGG + chr7.3:83364414-83364433 None:intergenic 50.0%
GAGAGCAAAGATGGGAGAGG+AGG - chr7.3:83365190-83365209 MS.gene44647:CDS 55.0%
GCAGAGAGCAAAGATGGGAG+AGG - chr7.3:83365187-83365206 MS.gene44647:CDS 55.0%
GGGAGAGGAGGGATAGAAGT+TGG - chr7.3:83365202-83365221 MS.gene44647:CDS 55.0%
! GTGGCGGGGTCTCATAGATT+TGG - chr7.3:83365082-83365101 MS.gene44647:CDS 55.0%
!!! TGATTTTGGTTGGGGGAGGC+CGG - chr7.3:83365117-83365136 MS.gene44647:CDS 55.0%
TGGTTGGGGGAGGCCGGAAA+AGG - chr7.3:83365123-83365142 MS.gene44647:CDS 65.0%
TTGGGGGAGGCCGGAAAAGG+TGG - chr7.3:83365126-83365145 MS.gene44647:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 83363879 83365739 83363879 ID=MS.gene44647
chr7.3 mRNA 83363879 83365739 83363879 ID=MS.gene44647.t1;Parent=MS.gene44647
chr7.3 exon 83364375 83365739 83364375 ID=MS.gene44647.t1.exon1;Parent=MS.gene44647.t1
chr7.3 CDS 83364375 83365739 83364375 ID=cds.MS.gene44647.t1;Parent=MS.gene44647.t1
chr7.3 exon 83363879 83363887 83363879 ID=MS.gene44647.t1.exon2;Parent=MS.gene44647.t1
chr7.3 CDS 83363879 83363887 83363879 ID=cds.MS.gene44647.t1;Parent=MS.gene44647.t1
Gene Sequence

>MS.gene44647

ATGGCTTCCAACAACAACAGCAACATTAAAGTTCACGATCACTTTAAGGTTGTTCCTCTATCATCAACAAAAACCACCTCAATCCCTCTCACTTTCTTCGACATATTTTGGCTAAGGTTCCATCCAGTAGAACGCGTTTTCTTCTACACCCTCCCAAATTCACAATCACACCCTTCTTTCTTCTTTCAAACAATTGTTCCGAATCTCAAATCATCACTCTCTTTAGCCCTCCAACATTTTCTCCCTTTAGCTGGTAACATTGTTTGGCCTTCTGATTCTTCAAAACCCATCATTCAATTTGATCCCAACAACGGTGGAGTTTCATTGATCATTGCAGAATGTGATTCAGATTTCAATCATGTCATTGAAAACCCACCCCATGAAGCTTCTTTGTCTCGTTCATTTATACCCCACTTAGAATCATCAGATTCTCTTGCTTCTATTTTGTCACTCCAAATCACCCTTTTCCCAAATAGTGGTTTTAGCATCGGAATCAGCACTCATCATGCTGTTCTTGATGGAAAATCTTCAACCATGTTCGTCAATGCTTGGGCTTATCTATGCAAAAAAGCTATTGAAGGAGATGAATCACAAACTTTGTTGTCAGAGTTCAAACCTTCATTCAATAGGGAAATCATCAAAGACCCAAACGGAAATAACGTAATGGACGTTGTATCCACATTATTCCCAAGTGAAAAAGGAAATGATAGAAGCTTAAAGATTTTCCCGTTTGAACCAAAACTCGAAGACTCTGTTCGTGCTACTTTTAAGCTCAAACATGAAGATTTGGATAAGATAAAACAAAGAGTGTTATCTACATGGGAAATATTCGATACGAAAGAATCAAAGCCACAAACTCTATCATCATTTATCATCACTTGTGCTTATTCGCTTGTTTGTGTTGCAAAAGCAATTCATGGGGCTCATAATGACAAAGAGAACTTTTCTTTTGTGTTCAGCGTAGATTGCAGAGCAAGGTTAGAGCCACCAATACCAAATAACTATCTTGGAAACTGTGTTTGGGCGTATTTCGTTGATACACAGCCGTTGGATTTTATAAAAGAAGATGGTGTTTTTTTAGTTGCTAAGAGTATTTATGAGAAAACAAAGATGATAAATGAAAAGGGTTTTCTTGAAGGGGCTATTAATGATATGTTTGAGAAACTTATTTCTTTATCAAGTGAAGGATTTGAAATAATGGGAGTGGCGGGGTCTCATAGATTTGGTGTTTATGAAATTGATTTTGGTTGGGGGAGGCCGGAAAAGGTGGAGATAGTGTCGATAGATAGAGGTGTAACAATTGGTTTGGCAGAGAGCAAAGATGGGAGAGGAGGGATAGAAGTTGGGCTTGTTCTTAATAAACCTGTAATGGATATCTTTAGCACTTTGTTTCTTGAAGGATTAACCATTAATGAGTAGTTCAACCATTACTCATTTTAATATTTTATTTTCAACAATTACTTTTTTAGAGGTGAAAATTTTGTTGTTCCTGCCACTTTATGTGTGGTTCATTTTCAATATAATGGGACTCGCATATTTTTCACTAGATAAAAAAGTGAGTGATGAAAAAAAAATGTTCGAATGAGTTTTGTTAACACTCCTCATGTACTCATGAATTGTAACAAGCAAGTGTAACTATGATGAGGAAAGAACATCAAGTTATTTCATTGATAATCAAAGTAACGTGCAAGATTGATTGTAAGATTTTCTTTCATTTTTCACTTTTTGATTCTTTTTTTTTTTTTCACTTTTTGATTCTATAACTTATACTGATTCGGGAAATTAATTTCTGGTTGTGAATTTACTGGCTGTTGTGAAATTAATTTTCTAGTTCTGGTTCTGAATTTTCTAGCTGTTGTGA
Protein sequence

>MS.gene44647.t1

MASNNNSNIKVHDHFKVVPLSSTKTTSIPLTFFDIFWLRFHPVERVFFYTLPNSQSHPSFFFQTIVPNLKSSLSLALQHFLPLAGNIVWPSDSSKPIIQFDPNNGGVSLIIAECDSDFNHVIENPPHEASLSRSFIPHLESSDSLASILSLQITLFPNSGFSIGISTHHAVLDGKSSTMFVNAWAYLCKKAIEGDESQTLLSEFKPSFNREIIKDPNGNNVMDVVSTLFPSEKGNDRSLKIFPFEPKLEDSVRATFKLKHEDLDKIKQRVLSTWEIFDTKESKPQTLSSFIITCAYSLVCVAKAIHGAHNDKENFSFVFSVDCRARLEPPIPNNYLGNCVWAYFVDTQPLDFIKEDGVFLVAKSIYEKTKMINEKGFLEGAINDMFEKLISLSSEGFEIMGVAGSHRFGVYEIDFGWGRPEKVEIVSIDRGVTIGLAESKDGRGGIEVGLVLNKPLL