AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene48581

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48581.t1 MTR_7g014200 94.505 455 25 0 1 455 1 455 0.0 882
MS.gene48581.t1 MTR_7g014160 73.043 460 115 4 1 453 1 458 0.0 688
MS.gene48581.t1 MTR_7g014610 73.866 463 110 5 1 454 1 461 0.0 675
MS.gene48581.t1 MTR_7g014620 64.989 437 126 5 21 454 6 418 0.0 540
MS.gene48581.t1 MTR_7g013850 59.442 466 168 9 6 454 5 466 0.0 523
MS.gene48581.t1 MTR_7g014630 68.227 299 87 2 1 291 1 299 7.26e-141 408
MS.gene48581.t1 MTR_6g015320 42.699 452 238 10 7 454 6 440 1.63e-109 332
MS.gene48581.t1 MTR_6g015315 43.946 446 236 8 10 454 6 438 1.00e-107 327
MS.gene48581.t1 MTR_7g014330 41.060 453 246 11 10 454 3 442 6.44e-105 320
MS.gene48581.t1 MTR_7g014230 41.447 456 246 11 7 454 2 444 6.68e-105 320
MS.gene48581.t1 MTR_6g015830 42.922 438 220 11 26 454 30 446 1.65e-104 319
MS.gene48581.t1 MTR_6g015850 42.166 434 226 10 26 454 30 443 1.90e-103 316
MS.gene48581.t1 MTR_6g015815 41.649 461 244 12 3 454 2 446 3.38e-103 315
MS.gene48581.t1 MTR_7g014250 39.956 453 251 11 10 454 3 442 3.34e-99 305
MS.gene48581.t1 MTR_7g014670 77.515 169 37 1 286 454 78 245 8.18e-90 274
MS.gene48581.t1 MTR_7g014180 77.841 176 36 2 1 176 1 173 2.17e-87 265
MS.gene48581.t1 MTR_2g089765 33.603 494 259 16 3 454 2 468 2.15e-71 234
MS.gene48581.t1 MTR_3g028210 35.000 460 268 13 9 454 8 450 7.49e-71 232
MS.gene48581.t1 MTR_2g089650 34.672 473 262 15 1 452 1 447 2.65e-68 225
MS.gene48581.t1 MTR_7g014360 35.864 382 210 9 78 454 21 372 2.01e-67 221
MS.gene48581.t1 MTR_3g087640 34.279 458 261 14 17 452 17 456 1.72e-65 218
MS.gene48581.t1 MTR_2g089755 34.389 442 252 11 30 452 37 459 1.75e-65 218
MS.gene48581.t1 MTR_2g089670 34.228 447 252 15 26 454 41 463 1.20e-63 213
MS.gene48581.t1 MTR_2g089735 35.831 427 237 16 43 452 39 445 6.16e-63 211
MS.gene48581.t1 MTR_8g012750 33.115 459 255 16 26 454 22 458 1.16e-59 202
MS.gene48581.t1 MTR_7g015860 31.250 480 264 15 6 454 2 446 1.85e-58 199
MS.gene48581.t1 MTR_6g015310 32.915 398 197 10 53 446 2 333 6.44e-57 191
MS.gene48581.t1 MTR_2g089665 27.974 454 186 14 9 442 2 334 5.14e-38 141
MS.gene48581.t1 MTR_7g014310 30.060 336 177 8 118 453 31 308 2.52e-35 133
MS.gene48581.t1 MTR_3g435300 36.574 216 110 7 17 218 11 213 3.64e-35 132
MS.gene48581.t1 MTR_7g086000 24.943 437 275 14 8 421 3 409 2.17e-22 99.8
MS.gene48581.t1 MTR_5g084870 25.943 424 257 16 29 444 25 399 6.34e-22 98.2
MS.gene48581.t1 MTR_6g045553 25.562 489 275 20 8 452 13 456 2.69e-21 96.7
MS.gene48581.t1 MTR_3g070170 25.052 479 297 20 3 452 6 451 3.51e-20 93.2
MS.gene48581.t1 MTR_8g075610 24.611 386 239 17 46 420 30 374 5.42e-20 92.4
MS.gene48581.t1 MTR_4g007540 24.528 424 268 18 9 420 3 386 2.90e-19 90.1
MS.gene48581.t1 MTR_5g084850 25.761 427 253 18 29 444 25 398 1.44e-18 88.2
MS.gene48581.t1 MTR_8g079950 24.299 428 265 15 29 444 25 405 2.28e-18 87.4
MS.gene48581.t1 MTR_4g094828 24.499 449 273 17 26 450 25 431 8.23e-18 85.9
MS.gene48581.t1 MTR_8g079980 25.117 426 263 13 29 444 25 404 8.97e-18 85.5
MS.gene48581.t1 MTR_4g022290 24.892 462 251 18 28 453 28 429 1.04e-17 85.5
MS.gene48581.t1 MTR_8g079970 23.239 426 272 15 29 444 25 405 1.53e-17 85.1
MS.gene48581.t1 MTR_3g086020 24.828 435 249 19 30 452 28 396 4.57e-17 83.6
MS.gene48581.t1 MTR_2g015430 24.819 415 260 16 27 432 22 393 7.45e-17 82.8
MS.gene48581.t1 MTR_3g088420 23.165 436 279 12 28 450 22 414 7.59e-17 82.8
MS.gene48581.t1 MTR_8g467580 22.464 414 262 14 21 420 17 385 1.12e-16 82.4
MS.gene48581.t1 MTR_4g097090 23.129 441 282 16 1 419 1 406 1.77e-16 82.0
MS.gene48581.t1 MTR_3g026310 23.041 434 297 12 28 450 22 429 1.29e-15 79.3
MS.gene48581.t1 MTR_4g022825 22.759 435 289 13 28 450 22 421 4.71e-15 77.4
MS.gene48581.t1 MTR_2g105330 22.897 428 269 19 9 420 3 385 5.92e-15 77.0
MS.gene48581.t1 MTR_7g084910 21.786 459 292 18 9 456 17 419 6.56e-15 77.0
MS.gene48581.t1 MTR_2g064495 22.664 428 270 19 9 420 3 385 1.39e-14 75.9
MS.gene48581.t1 MTR_7g084940 22.328 421 276 14 9 421 16 393 1.77e-14 75.5
MS.gene48581.t1 MTR_7g085930 25.714 350 209 16 9 341 6 321 1.91e-14 75.5
MS.gene48581.t1 MTR_7g085990 24.857 350 212 16 9 341 2 317 2.02e-14 75.5
MS.gene48581.t1 MTR_7g014205 35.714 112 60 2 319 430 15 114 2.85e-14 69.7
MS.gene48581.t1 MTR_3g049150 23.160 462 287 19 9 442 2 423 4.00e-14 74.7
MS.gene48581.t1 MTR_3g102010 23.934 422 275 16 30 443 30 413 6.00e-14 73.9
MS.gene48581.t1 MTR_0190s0020 24.176 455 271 19 20 450 19 423 9.37e-14 73.6
MS.gene48581.t1 MTR_7g014340 52.113 71 28 2 10 77 3 70 1.99e-13 66.6
MS.gene48581.t1 MTR_7g014220 59.259 54 19 1 26 79 14 64 2.13e-13 65.9
MS.gene48581.t1 MTR_3g101960 22.886 402 265 15 30 419 29 397 3.07e-13 72.0
MS.gene48581.t1 MTR_3g102020 24.627 402 263 16 33 424 32 403 3.13e-13 71.6
MS.gene48581.t1 MTR_1g053315 22.078 385 257 12 46 420 58 409 4.45e-13 71.2
MS.gene48581.t1 MTR_3g101900 24.634 410 253 20 30 423 30 399 5.50e-13 70.9
MS.gene48581.t1 MTR_2g007880 23.041 434 255 17 52 454 48 433 5.25e-12 68.2
MS.gene48581.t1 MTR_4g022720 23.737 396 260 15 66 450 52 416 2.20e-11 65.9
MS.gene48581.t1 MTR_3g465980 23.575 386 252 12 9 385 10 361 2.38e-11 65.9
MS.gene48581.t1 MTR_4g094832 27.987 318 174 15 148 450 134 411 3.51e-11 65.5
MS.gene48581.t1 MTR_3g465910 23.810 336 222 8 8 338 9 315 4.62e-11 65.1
MS.gene48581.t1 MTR_4g094842 24.485 388 219 19 66 424 59 401 5.01e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48581.t1 AT5G39050 40.435 460 248 14 7 454 8 453 7.84e-94 291
MS.gene48581.t1 AT3G29670 39.456 441 237 11 20 454 19 435 9.15e-93 288
MS.gene48581.t1 AT5G39080 38.612 461 254 15 7 454 3 447 1.11e-91 286
MS.gene48581.t1 AT3G29590 38.445 463 242 11 4 454 3 434 2.50e-91 285
MS.gene48581.t1 AT5G39090 37.868 441 241 14 21 454 19 433 4.58e-87 273
MS.gene48581.t1 AT3G29635 36.490 433 250 9 28 454 27 440 7.87e-87 273
MS.gene48581.t1 AT5G61160 34.793 434 256 10 28 454 23 436 5.85e-78 250
MS.gene48581.t1 AT3G29680 34.157 445 241 11 28 454 23 433 1.85e-73 238
MS.gene48581.t1 AT1G03940 32.527 455 265 14 19 454 19 450 5.95e-64 214
MS.gene48581.t1 AT1G03495 32.836 335 193 10 19 340 19 334 1.35e-44 159
MS.gene48581.t1 AT5G23940 26.164 451 263 18 30 452 25 433 1.74e-26 112
MS.gene48581.t1 AT3G29720 31.579 209 118 8 105 304 24 216 6.63e-21 91.3
MS.gene48581.t1 AT5G48930 25.116 430 258 20 9 420 3 386 2.07e-20 93.6
MS.gene48581.t1 AT5G67150 26.536 407 255 14 28 424 27 399 2.11e-18 87.8
MS.gene48581.t1 AT2G19070 22.864 433 278 16 9 419 4 402 7.59e-18 85.9
MS.gene48581.t1 AT1G28680 23.204 362 237 11 69 423 62 389 8.16e-17 82.8
MS.gene48581.t1 AT5G57840 24.197 467 288 21 9 454 3 424 3.53e-16 80.9
MS.gene48581.t1 AT2G39980 22.766 470 309 16 16 453 15 462 3.55e-16 81.3
MS.gene48581.t1 AT5G41040 24.650 357 233 14 69 420 69 394 4.35e-16 80.5
MS.gene48581.t1 AT5G41040 24.650 357 233 14 69 420 85 410 5.52e-16 80.5
MS.gene48581.t1 AT5G07850 24.009 429 274 14 16 424 9 405 7.33e-16 80.1
MS.gene48581.t1 AT3G29690 30.323 155 68 5 317 454 33 164 8.24e-16 75.9
MS.gene48581.t1 AT1G28680 24.555 281 176 8 69 343 62 312 1.12e-14 75.9
MS.gene48581.t1 AT5G42830 25.474 369 231 17 64 419 59 396 1.37e-12 69.7
MS.gene48581.t1 AT3G62160 22.717 427 266 18 18 434 11 383 1.73e-12 69.3
MS.gene48581.t1 AT2G23510 21.951 369 245 17 69 428 69 403 2.14e-11 66.2
MS.gene48581.t1 AT5G07860 23.624 436 282 17 33 455 34 431 4.93e-11 65.1
MS.gene48581.t1 AT3G47170 25.890 309 175 13 49 338 57 330 5.52e-11 65.1
MS.gene48581.t1 AT4G31910 22.963 405 231 15 12 389 24 374 5.84e-11 64.7
MS.gene48581.t1 AT2G40230 22.173 451 294 18 6 442 2 409 9.40e-11 63.9
MS.gene48581.t1 AT3G48720 21.978 364 232 14 69 420 61 384 9.40e-11 63.9

Find 84 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAAGTTCACGATCACTTTA+AGG 0.164541 7.2:-83032936 MS.gene48581:CDS
GAAGGGTGTGATTGTGAATT+TGG 0.182607 7.2:+83032809 None:intergenic
TCACTTTCTTCGACATATTT+TGG 0.186190 7.2:-83032874 MS.gene48581:CDS
TCCATTACGTTATTTCCTTT+TGG 0.190336 7.2:+83032317 None:intergenic
CGAACAGAGTCTTCGAGTTT+TGG 0.241278 7.2:+83032227 None:intergenic
CTTTAGCTGGTAACATTGTT+TGG 0.264357 7.2:-83032718 MS.gene48581:CDS
AGTTCGAACCTTCATTCAAT+AGG 0.303200 7.2:-83032355 MS.gene48581:CDS
CAGTTTCCAAGATAGTTATT+TGG 0.312431 7.2:+83031969 None:intergenic
TAAGCTCAAACATGAAGATT+TGG 0.316500 7.2:-83032195 MS.gene48581:CDS
ACATTATTCCCAAGTGAAAA+AGG 0.317573 7.2:-83032284 MS.gene48581:CDS
ACTATCTTGGAAACTGTGTT+TGG 0.321806 7.2:-83031962 MS.gene48581:CDS
GGTGTTTATGAGATTGATTT+TGG 0.325995 7.2:-83031738 MS.gene48581:CDS
AAGTGAAGGATTTGAAATAA+TGG 0.333426 7.2:-83031784 MS.gene48581:CDS
CCAAGATAGTTATTTGGTAT+TGG 0.355456 7.2:+83031975 None:intergenic
GGGAGAGGAGGGATAGAAGT+TGG 0.357090 7.2:-83031639 MS.gene48581:CDS
AACCATGTTCGTCAAAGCTT+GGG 0.374698 7.2:-83032432 MS.gene48581:CDS
ACTCATCATGCTGTTCTTGA+TGG 0.374958 7.2:-83032464 MS.gene48581:CDS
AACAAAGATGATAAATGAAA+AGG 0.386871 7.2:-83031859 MS.gene48581:CDS
AGTGAAGGATTTGAAATAAT+GGG 0.394576 7.2:-83031783 MS.gene48581:CDS
CCAATACCAAATAACTATCT+TGG 0.397200 7.2:-83031975 MS.gene48581:CDS
TTTAATGTTGCTGTTGTTGT+TGG 0.400453 7.2:+83032955 None:intergenic
ATAGATAGAGGTTTAACAAT+TGG 0.401286 7.2:-83031681 MS.gene48581:CDS
TAGAGGTTTAACAATTGGTT+TGG 0.408672 7.2:-83031676 MS.gene48581:CDS
CAACCATGTTCGTCAAAGCT+TGG 0.410362 7.2:-83032433 MS.gene48581:CDS
AACGAGACAAAGAAGCTTCA+TGG 0.411299 7.2:+83032585 None:intergenic
GAGAGTGATGATTTGAGATT+CGG 0.414444 7.2:+83032764 None:intergenic
AAGGGTGTGATTGTGAATTT+GGG 0.425297 7.2:+83032810 None:intergenic
GTTCGAACCTTCATTCAATA+GGG 0.432515 7.2:-83032354 MS.gene48581:CDS
ATTGTTTGAAAGAAGAAAGA+AGG 0.435032 7.2:+83032791 None:intergenic
ACAAAGATGATAAATGAAAA+GGG 0.439203 7.2:-83031858 MS.gene48581:CDS
CTATCTTGGAAACTGTGTTT+GGG 0.444763 7.2:-83031961 MS.gene48581:CDS
GGTTTGGCAGAGAGCAAAGA+TGG 0.455777 7.2:-83031660 MS.gene48581:CDS
ACAAATGATGATAGAGTTTG+TGG 0.455784 7.2:+83032113 None:intergenic
AATGAAACTCCACCGTCGTT+GGG 0.462138 7.2:+83032659 None:intergenic
TGTGTTGCAAAAGCAATTCA+TGG 0.469489 7.2:-83032065 MS.gene48581:CDS
GGAGAGGAGGGATAGAAGTT+GGG 0.470973 7.2:-83031638 MS.gene48581:CDS
AAAACCACTATTTGGGAAAA+GGG 0.472692 7.2:+83032502 None:intergenic
GAGATAGTGTCTATAGATAG+AGG 0.473823 7.2:-83031693 MS.gene48581:CDS
CTCATTTCTTTATCAAGTGA+AGG 0.475428 7.2:-83031798 MS.gene48581:CDS
ACGATTTCCCTATTGAATGA+AGG 0.477830 7.2:+83032347 None:intergenic
TGGTTGGGGAAGGCCGGAAA+AGG 0.479363 7.2:-83031718 MS.gene48581:CDS
CAAACAATGTTACCAGCTAA+AGG 0.481339 7.2:+83032719 None:intergenic
TAGAAGAAAACGCGTTCCAC+TGG 0.483101 7.2:+83032839 None:intergenic
GTTGGGATCAAATTGAATGA+TGG 0.484061 7.2:+83032676 None:intergenic
AGATAGTTATTTGGTATTGG+TGG 0.484142 7.2:+83031978 None:intergenic
AGCAAAGAGTGTTATCTACA+TGG 0.487585 7.2:-83032163 MS.gene48581:CDS
TTGGGATCAAATTGAATGAT+GGG 0.489257 7.2:+83032677 None:intergenic
GTGGCGGGGTCTCATAGATT+TGG 0.491756 7.2:-83031759 MS.gene48581:CDS
GAAGAAAGTGAGAGGGATTG+AGG 0.492974 7.2:+83032886 None:intergenic
CAATGAAACTCCACCGTCGT+TGG 0.494886 7.2:+83032658 None:intergenic
TTTGAAATAATGGGAGTGGC+GGG 0.497904 7.2:-83031774 MS.gene48581:CDS
AAACAATGTTACCAGCTAAA+GGG 0.504045 7.2:+83032720 None:intergenic
GTGTTGCAAAAGCAATTCAT+GGG 0.505428 7.2:-83032064 MS.gene48581:CDS
CAGCTAAAGGGAGAAAATGT+TGG 0.510508 7.2:+83032732 None:intergenic
ACGAGACAAAGAAGCTTCAT+GGG 0.511639 7.2:+83032586 None:intergenic
TTGGCTAAGGTTCCATCCAG+TGG 0.540296 7.2:-83032855 MS.gene48581:CDS
GAGAGCAAAGATGGGAGAGG+AGG 0.548569 7.2:-83031651 MS.gene48581:CDS
CCCAAAAGGAAATAACGTAA+TGG 0.551373 7.2:-83032318 MS.gene48581:CDS
TTGTTTGAAAGAAGAAAGAA+GGG 0.551836 7.2:+83032792 None:intergenic
AGCCCAAGCTTTGACGAACA+TGG 0.552028 7.2:+83032430 None:intergenic
TTGGGGAAGGCCGGAAAAGG+TGG 0.556102 7.2:-83031715 MS.gene48581:CDS
GAAATCGTCAAAGACCCAAA+AGG 0.559440 7.2:-83032332 MS.gene48581:CDS
AGAAAACGCGTTCCACTGGA+TGG 0.567045 7.2:+83032843 None:intergenic
AGAGCAAAGATGGGAGAGGA+GGG 0.573224 7.2:-83031650 MS.gene48581:CDS
TTTCGTTGATACACAGCCGT+TGG 0.584829 7.2:-83031934 MS.gene48581:CDS
AGGATTTGAAATAATGGGAG+TGG 0.589904 7.2:-83031778 MS.gene48581:CDS
GCAAAGAGTGTTATCTACAT+GGG 0.593050 7.2:-83032162 MS.gene48581:CDS
GCAGAGAGCAAAGATGGGAG+AGG 0.598380 7.2:-83031654 MS.gene48581:CDS
GTTTGGCAGAGAGCAAAGAT+GGG 0.598479 7.2:-83031659 MS.gene48581:CDS
GAAAGTGAGAGGGATTGAGG+TGG 0.600737 7.2:+83032889 None:intergenic
GTGTGATTGTGAATTTGGGA+GGG 0.604440 7.2:+83032814 None:intergenic
GAGAATCTGATGATTCTAAG+TGG 0.611695 7.2:+83032552 None:intergenic
ATTTGAAATAATGGGAGTGG+CGG 0.616341 7.2:-83031775 MS.gene48581:CDS
GGTGTGATTGTGAATTTGGG+AGG 0.618307 7.2:+83032813 None:intergenic
CTAAAGGGAGAAAATGTTGG+AGG 0.619278 7.2:+83032735 None:intergenic
TCAGCGTAGATTGCAGAGCA+AGG 0.630412 7.2:-83032007 MS.gene48581:CDS
CAATTTGATCCCAACGACGG+TGG 0.633422 7.2:-83032668 MS.gene48581:CDS
AATATGTCGAAGAAAGTGAG+AGG 0.638159 7.2:+83032878 None:intergenic
TTGAAATAATGGGAGTGGCG+GGG 0.646292 7.2:-83031773 MS.gene48581:CDS
TGTTGCAAAAGCAATTCATG+GGG 0.654042 7.2:-83032063 MS.gene48581:CDS
ATTCAATTTGATCCCAACGA+CGG 0.666911 7.2:-83032671 MS.gene48581:CDS
TAAAGGGAGAAAATGTTGGA+GGG 0.667165 7.2:+83032736 None:intergenic
ATATGTCGAAGAAAGTGAGA+GGG 0.668848 7.2:+83032879 None:intergenic
CGAGACAAAGAAGCTTCATG+GGG 0.713707 7.2:+83032587 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATATTAAAATGAGTAA+TGG + chr7.2:83032595-83032614 None:intergenic 10.0%
!! AACAAAGATGATAAATGAAA+AGG - chr7.2:83032271-83032290 MS.gene48581:CDS 20.0%
!! ACAAAGATGATAAATGAAAA+GGG - chr7.2:83032272-83032291 MS.gene48581:CDS 20.0%
!!! TCTTCTTTTATAAAATCCAA+CGG + chr7.2:83032215-83032234 None:intergenic 20.0%
!!! TGAAATTAATTTTCTAGTTC+TGG - chr7.2:83032956-83032975 MS.gene48581:CDS 20.0%
!!! TGGTTCATTTTCAATATAAT+GGG - chr7.2:83032672-83032691 MS.gene48581:CDS 20.0%
!!! TTCAACAATTACTTTTTTAG+AGG - chr7.2:83032617-83032636 MS.gene48581:CDS 20.0%
!!! TTCTATAACTTATACTGATT+CGG - chr7.2:83032897-83032916 MS.gene48581:CDS 20.0%
!!! TTGGATTTTATAAAAGAAGA+TGG - chr7.2:83032215-83032234 MS.gene48581:CDS 20.0%
! ATAGATAGAGGTTTAACAAT+TGG - chr7.2:83032449-83032468 MS.gene48581:CDS 25.0%
! ATTGTTTGAAAGAAGAAAGA+AGG + chr7.2:83031342-83031361 None:intergenic 25.0%
! TGTTCTTAATAAACCTGTAA+TGG - chr7.2:83032517-83032536 MS.gene48581:CDS 25.0%
! TTGTTTGAAAGAAGAAAGAA+GGG + chr7.2:83031341-83031360 None:intergenic 25.0%
!! AAGTGAAGGATTTGAAATAA+TGG - chr7.2:83032346-83032365 MS.gene48581:CDS 25.0%
!! AGTGAAGGATTTGAAATAAT+GGG - chr7.2:83032347-83032366 MS.gene48581:CDS 25.0%
!! GTGGTTCATTTTCAATATAA+TGG - chr7.2:83032671-83032690 MS.gene48581:CDS 25.0%
!!! TATCATTTCCTTTTTCACTT+GGG + chr7.2:83031857-83031876 None:intergenic 25.0%
!!! TCTATAACTTATACTGATTC+GGG - chr7.2:83032898-83032917 MS.gene48581:CDS 25.0%
!!! TTATGAGATTGATTTTGGTT+GGG - chr7.2:83032397-83032416 MS.gene48581:CDS 25.0%
!!! TTTATGAGATTGATTTTGGT+TGG - chr7.2:83032396-83032415 MS.gene48581:CDS 25.0%
AAAAACCACTATTTGGGAAA+AGG + chr7.2:83031632-83031651 None:intergenic 30.0%
AAAACCACTATTTGGGAAAA+GGG + chr7.2:83031631-83031650 None:intergenic 30.0%
AAAATGAACCACAGATAAAG+TGG + chr7.2:83032663-83032682 None:intergenic 30.0%
AAACAATGTTACCAGCTAAA+GGG + chr7.2:83031413-83031432 None:intergenic 30.0%
ACAAATGATGATAGAGTTTG+TGG + chr7.2:83032020-83032039 None:intergenic 30.0%
ACATTATTCCCAAGTGAAAA+AGG - chr7.2:83031846-83031865 MS.gene48581:CDS 30.0%
ATGGTTGAACTACTCATTAA+TGG + chr7.2:83032576-83032595 None:intergenic 30.0%
CAATGCAAAAACCACTATTT+GGG + chr7.2:83031638-83031657 None:intergenic 30.0%
CAGTTTCCAAGATAGTTATT+TGG + chr7.2:83032164-83032183 None:intergenic 30.0%
CCAAGATAGTTATTTGGTAT+TGG + chr7.2:83032158-83032177 None:intergenic 30.0%
CCAATACCAAATAACTATCT+TGG - chr7.2:83032155-83032174 MS.gene48581:CDS 30.0%
CTATGCAAAAAAGCTATTGA+AGG - chr7.2:83031726-83031745 MS.gene48581:CDS 30.0%
CTCATTTCTTTATCAAGTGA+AGG - chr7.2:83032332-83032351 MS.gene48581:CDS 30.0%
TAAAGTTCACGATCACTTTA+AGG - chr7.2:83031194-83031213 MS.gene48581:intron 30.0%
TAAGCTCAAACATGAAGATT+TGG - chr7.2:83031935-83031954 MS.gene48581:CDS 30.0%
TAGAGGTTTAACAATTGGTT+TGG - chr7.2:83032454-83032473 MS.gene48581:CDS 30.0%
TTACAATTCATGAGTACATG+AGG + chr7.2:83032770-83032789 None:intergenic 30.0%
TTGGGATCAAATTGAATGAT+GGG + chr7.2:83031456-83031475 None:intergenic 30.0%
! AATGAAAAGGGTTTTCTTGA+AGG - chr7.2:83032284-83032303 MS.gene48581:CDS 30.0%
! AGATAGTTATTTGGTATTGG+TGG + chr7.2:83032155-83032174 None:intergenic 30.0%
! ATGAAAAGGGTTTTCTTGAA+GGG - chr7.2:83032285-83032304 MS.gene48581:CDS 30.0%
! TCACTTTCTTCGACATATTT+TGG - chr7.2:83031256-83031275 MS.gene48581:intron 30.0%
! TGATTCGGGAAATTAATTTC+TGG - chr7.2:83032912-83032931 MS.gene48581:CDS 30.0%
! TTTAATGTTGCTGTTGTTGT+TGG + chr7.2:83031178-83031197 None:intergenic 30.0%
! TTTAGCACTTTGTTTCTTGA+AGG - chr7.2:83032545-83032564 MS.gene48581:CDS 30.0%
! TTTTTCACTTGGGAATAATG+TGG + chr7.2:83031847-83031866 None:intergenic 30.0%
!! ATTTCTGGTTCTGAATTTAC+TGG - chr7.2:83032927-83032946 MS.gene48581:CDS 30.0%
!! CCATTACGTTATTTCCTTTT+GGG + chr7.2:83031815-83031834 None:intergenic 30.0%
!! CTATCATTTCCTTTTTCACT+TGG + chr7.2:83031858-83031877 None:intergenic 30.0%
!! GGTGTTTATGAGATTGATTT+TGG - chr7.2:83032392-83032411 MS.gene48581:CDS 30.0%
!! TCCATTACGTTATTTCCTTT+TGG + chr7.2:83031816-83031835 None:intergenic 30.0%
!!! AATGATGGGTTTTGAAGAAT+CGG + chr7.2:83031442-83031461 None:intergenic 30.0%
!!! TATGAGATTGATTTTGGTTG+GGG - chr7.2:83032398-83032417 MS.gene48581:CDS 30.0%
AAGCAAGTGTAACTATGATG+AGG - chr7.2:83032791-83032810 MS.gene48581:CDS 35.0%
AATATGTCGAAGAAAGTGAG+AGG + chr7.2:83031255-83031274 None:intergenic 35.0%
ACGATTTCCCTATTGAATGA+AGG + chr7.2:83031786-83031805 None:intergenic 35.0%
ACTATCTTGGAAACTGTGTT+TGG - chr7.2:83032168-83032187 MS.gene48581:CDS 35.0%
AGTTCGAACCTTCATTCAAT+AGG - chr7.2:83031775-83031794 MS.gene48581:CDS 35.0%
ATATGTCGAAGAAAGTGAGA+GGG + chr7.2:83031254-83031273 None:intergenic 35.0%
ATTCAATTTGATCCCAACGA+CGG - chr7.2:83031459-83031478 MS.gene48581:intron 35.0%
CAAACAATGTTACCAGCTAA+AGG + chr7.2:83031414-83031433 None:intergenic 35.0%
CCAATGCAAAAACCACTATT+TGG + chr7.2:83031639-83031658 None:intergenic 35.0%
CCCAAAAGGAAATAACGTAA+TGG - chr7.2:83031812-83031831 MS.gene48581:CDS 35.0%
CTATCTTGGAAACTGTGTTT+GGG - chr7.2:83032169-83032188 MS.gene48581:CDS 35.0%
CTTTAGCTGGTAACATTGTT+TGG - chr7.2:83031412-83031431 MS.gene48581:intron 35.0%
GAGAATCTGATGATTCTAAG+TGG + chr7.2:83031581-83031600 None:intergenic 35.0%
GAGATAGTGTCTATAGATAG+AGG - chr7.2:83032437-83032456 MS.gene48581:CDS 35.0%
GTGCTAAAGATATCCATTAC+AGG + chr7.2:83032533-83032552 None:intergenic 35.0%
GTGTTGCAAAAGCAATTCAT+GGG - chr7.2:83032066-83032085 MS.gene48581:CDS 35.0%
GTTCGAACCTTCATTCAATA+GGG - chr7.2:83031776-83031795 MS.gene48581:CDS 35.0%
GTTGGGATCAAATTGAATGA+TGG + chr7.2:83031457-83031476 None:intergenic 35.0%
TAAAGGGAGAAAATGTTGGA+GGG + chr7.2:83031397-83031416 None:intergenic 35.0%
TGTGTTGCAAAAGCAATTCA+TGG - chr7.2:83032065-83032084 MS.gene48581:CDS 35.0%
TGTTGCAAAAGCAATTCATG+GGG - chr7.2:83032067-83032086 MS.gene48581:CDS 35.0%
! AAGGGTGTGATTGTGAATTT+GGG + chr7.2:83031323-83031342 None:intergenic 35.0%
! AGGATTTGAAATAATGGGAG+TGG - chr7.2:83032352-83032371 MS.gene48581:CDS 35.0%
! ATTTGAAATAATGGGAGTGG+CGG - chr7.2:83032355-83032374 MS.gene48581:CDS 35.0%
! CCAAATAGTGGTTTTTGCAT+TGG - chr7.2:83031636-83031655 MS.gene48581:CDS 35.0%
! GAGAGTGATGATTTGAGATT+CGG + chr7.2:83031369-83031388 None:intergenic 35.0%
! TGAAAAGGGTTTTCTTGAAG+GGG - chr7.2:83032286-83032305 MS.gene48581:CDS 35.0%
!! AGCAAAGAGTGTTATCTACA+TGG - chr7.2:83031967-83031986 MS.gene48581:CDS 35.0%
!! GCAAAGAGTGTTATCTACAT+GGG - chr7.2:83031968-83031987 MS.gene48581:CDS 35.0%
!!! GTGGTTTTTGTTGATGATAG+AGG + chr7.2:83031225-83031244 None:intergenic 35.0%
!!! TCTTCGACATATTTTGGCTA+AGG - chr7.2:83031262-83031281 MS.gene48581:intron 35.0%
!!! TCTTCGAGTTTTGGTTCAAA+CGG + chr7.2:83031897-83031916 None:intergenic 35.0%
AACCATGTTCGTCAAAGCTT+GGG - chr7.2:83031698-83031717 MS.gene48581:CDS 40.0%
AACGAGACAAAGAAGCTTCA+TGG + chr7.2:83031548-83031567 None:intergenic 40.0%
ACGAGACAAAGAAGCTTCAT+GGG + chr7.2:83031547-83031566 None:intergenic 40.0%
ACTCATCATGCTGTTCTTGA+TGG - chr7.2:83031666-83031685 MS.gene48581:CDS 40.0%
CAGCTAAAGGGAGAAAATGT+TGG + chr7.2:83031401-83031420 None:intergenic 40.0%
CTAAAGGGAGAAAATGTTGG+AGG + chr7.2:83031398-83031417 None:intergenic 40.0%
GAAATCGTCAAAGACCCAAA+AGG - chr7.2:83031798-83031817 MS.gene48581:CDS 40.0%
GTGTGATTGTGAATTTGGGA+GGG + chr7.2:83031319-83031338 None:intergenic 40.0%
! ATCACCCTTTTCCCAAATAG+TGG - chr7.2:83031624-83031643 MS.gene48581:CDS 40.0%
! CAACATTTTCTCCCTTTAGC+TGG - chr7.2:83031399-83031418 MS.gene48581:intron 40.0%
! GAAGGGTGTGATTGTGAATT+TGG + chr7.2:83031324-83031343 None:intergenic 40.0%
! TTTGAAATAATGGGAGTGGC+GGG - chr7.2:83032356-83032375 MS.gene48581:CDS 40.0%
!!! AGATTGATTTTGGTTGGGGA+AGG - chr7.2:83032402-83032421 MS.gene48581:CDS 40.0%
!!! ATGGGTTTTGAAGAATCGGA+AGG + chr7.2:83031438-83031457 None:intergenic 40.0%
!!! CTTCGAGTTTTGGTTCAAAC+GGG + chr7.2:83031896-83031915 None:intergenic 40.0%
AATGAAACTCCACCGTCGTT+GGG + chr7.2:83031474-83031493 None:intergenic 45.0%
CAACCATGTTCGTCAAAGCT+TGG - chr7.2:83031697-83031716 MS.gene48581:CDS 45.0%
CGAGACAAAGAAGCTTCATG+GGG + chr7.2:83031546-83031565 None:intergenic 45.0%
GAAGAAAGTGAGAGGGATTG+AGG + chr7.2:83031247-83031266 None:intergenic 45.0%
GGTGTGATTGTGAATTTGGG+AGG + chr7.2:83031320-83031339 None:intergenic 45.0%
GTTTGGCAGAGAGCAAAGAT+GGG - chr7.2:83032471-83032490 MS.gene48581:CDS 45.0%
TAGAAGAAAACGCGTTCCAC+TGG + chr7.2:83031294-83031313 None:intergenic 45.0%
TGAACCACAGATAAAGTGGC+AGG + chr7.2:83032659-83032678 None:intergenic 45.0%
TTTCGTTGATACACAGCCGT+TGG - chr7.2:83032196-83032215 MS.gene48581:CDS 45.0%
! GACACTATCTCCACCTTTTC+CGG + chr7.2:83032428-83032447 None:intergenic 45.0%
! TTGAAATAATGGGAGTGGCG+GGG - chr7.2:83032357-83032376 MS.gene48581:CDS 45.0%
!! CGAACAGAGTCTTCGAGTTT+TGG + chr7.2:83031906-83031925 None:intergenic 45.0%
!! TGTTCCTGCCACTTTATCTG+TGG - chr7.2:83032652-83032671 MS.gene48581:CDS 45.0%
AGAAAACGCGTTCCACTGGA+TGG + chr7.2:83031290-83031309 None:intergenic 50.0%
AGAGCAAAGATGGGAGAGGA+GGG - chr7.2:83032480-83032499 MS.gene48581:CDS 50.0%
CAATGAAACTCCACCGTCGT+TGG + chr7.2:83031475-83031494 None:intergenic 50.0%
CAATTTGATCCCAACGACGG+TGG - chr7.2:83031462-83031481 MS.gene48581:intron 50.0%
GAAAGTGAGAGGGATTGAGG+TGG + chr7.2:83031244-83031263 None:intergenic 50.0%
GGAGAGGAGGGATAGAAGTT+GGG - chr7.2:83032492-83032511 MS.gene48581:CDS 50.0%
GGTTTGGCAGAGAGCAAAGA+TGG - chr7.2:83032470-83032489 MS.gene48581:CDS 50.0%
TCAGCGTAGATTGCAGAGCA+AGG - chr7.2:83032123-83032142 MS.gene48581:CDS 50.0%
TTGGCTAAGGTTCCATCCAG+TGG - chr7.2:83031275-83031294 MS.gene48581:intron 50.0%
! AGCCCAAGCTTTGACGAACA+TGG + chr7.2:83031703-83031722 None:intergenic 50.0%
!!! TGATTTTGGTTGGGGAAGGC+CGG - chr7.2:83032406-83032425 MS.gene48581:CDS 50.0%
GAGAGCAAAGATGGGAGAGG+AGG - chr7.2:83032479-83032498 MS.gene48581:CDS 55.0%
GCAGAGAGCAAAGATGGGAG+AGG - chr7.2:83032476-83032495 MS.gene48581:CDS 55.0%
GGGAGAGGAGGGATAGAAGT+TGG - chr7.2:83032491-83032510 MS.gene48581:CDS 55.0%
! GTGGCGGGGTCTCATAGATT+TGG - chr7.2:83032371-83032390 MS.gene48581:CDS 55.0%
TGGTTGGGGAAGGCCGGAAA+AGG - chr7.2:83032412-83032431 MS.gene48581:CDS 60.0%
TTGGGGAAGGCCGGAAAAGG+TGG - chr7.2:83032415-83032434 MS.gene48581:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 83031168 83032984 83031168 ID=MS.gene48581
chr7.2 mRNA 83031168 83032984 83031168 ID=MS.gene48581.t1;Parent=MS.gene48581
chr7.2 exon 83031620 83032984 83031620 ID=MS.gene48581.t1.exon1;Parent=MS.gene48581.t1
chr7.2 CDS 83031620 83032984 83031620 ID=cds.MS.gene48581.t1;Parent=MS.gene48581.t1
chr7.2 exon 83031168 83031179 83031168 ID=MS.gene48581.t1.exon2;Parent=MS.gene48581.t1
chr7.2 CDS 83031168 83031179 83031168 ID=cds.MS.gene48581.t1;Parent=MS.gene48581.t1
Gene Sequence

>MS.gene48581

ATGGCTTCCAACAACAACAGCAACATTAAAGTTCACGATCACTTTAAGGTTGTTCCTCTATCATCAACAAAAACCACCTCAATCCCTCTCACTTTCTTCGACATATTTTGGCTAAGGTTCCATCCAGTGGAACGCGTTTTCTTCTACACCCTCCCAAATTCACAATCACACCCTTCTTTCTTCTTTCAAACAATTGTTCCGAATCTCAAATCATCACTCTCTTTAACCCTCCAACATTTTCTCCCTTTAGCTGGTAACATTGTTTGGCCTTCCGATTCTTCAAAACCCATCATTCAATTTGATCCCAACGACGGTGGAGTTTCATTGATCATTGCAGAATGTGATTCAGATTTCAATCATGTCATTGAAAACTCACCCCATGAAGCTTCTTTGTCTCGTTCTTTTATACACCACTTAGAATCATCAGATTCTCTTGCTTCTATTATGTCACTTCAAATCACCCTTTTCCCAAATAGTGGTTTTTGCATTGGAATCAGCACTCATCATGCTGTTCTTGATGGAAAATCTTCAACCATGTTCGTCAAAGCTTGGGCTTATCTATGCAAAAAAGCTATTGAAGGAGATGAATCACAAACTTTGTTGTCAGAGTTCGAACCTTCATTCAATAGGGAAATCGTCAAAGACCCAAAAGGAAATAACGTAATGGACATTGTATCCACATTATTCCCAAGTGAAAAAGGAAATGATAGAAGCTTAAAGATTTTCCCGTTTGAACCAAAACTCGAAGACTCTGTTCGTGCTACTTTTAAGCTCAAACATGAAGATTTGGATAAGATAAAGCAAAGAGTGTTATCTACATGGGAAATATTCGATACGAAAGAATCAAAGCCACAAACTCTATCATCATTTGTCATCACTTGTGCTTATTCGCTTGTTTGTGTTGCAAAAGCAATTCATGGGGCTCATAATGACAAAGAGAAGTTTTCTTTTGTGTTCAGCGTAGATTGCAGAGCAAGGTTAGAACCACCAATACCAAATAACTATCTTGGAAACTGTGTTTGGGCGTATTTCGTTGATACACAGCCGTTGGATTTTATAAAAGAAGATGGTGTTTTTTTAGTTGCTAAGAGTATTTATGAGAAAACAAAGATGATAAATGAAAAGGGTTTTCTTGAAGGGGCTATTAATGATATGTTTGAGAAACTCATTTCTTTATCAAGTGAAGGATTTGAAATAATGGGAGTGGCGGGGTCTCATAGATTTGGTGTTTATGAGATTGATTTTGGTTGGGGAAGGCCGGAAAAGGTGGAGATAGTGTCTATAGATAGAGGTTTAACAATTGGTTTGGCAGAGAGCAAAGATGGGAGAGGAGGGATAGAAGTTGGGCTTGTTCTTAATAAACCTGTAATGGATATCTTTAGCACTTTGTTTCTTGAAGGATTATCCATTAATGAGTAGTTCAACCATTACTCATTTTAATATTTTATTTTCAACAATTACTTTTTTAGAGGTGAAAATTTTGTTGTTCCTGCCACTTTATCTGTGGTTCATTTTCAATATAATGGGACTCGCATATTTTTCACTACATAAAAAAGTGAGTGATGAAAAAAAAATGTTCGAACGAGTTTTGTTAACACTCCTCATGTACTCATGAATTGTAACAAGCAAGTGTAACTATGATGAGGAAAGAACATCAAGTTATTTCATTGATAATCAAAGTAACGTGCAAGATTGATTGTAAGATTTTCTTTCATTTTTCACTTTTTGATTCTATAACTTATACTGATTCGGGAAATTAATTTCTGGTTCTGAATTTACTGGCTGTTGTGAAATTAATTTTCTAGTTCTGGTTCTGA
Protein sequence

>MS.gene48581.t1

MASNNNSNIKVHDHFKVVPLSSTKTTSIPLTFFDIFWLRFHPVERVFFYTLPNSQSHPSFFFQTIVPNLKSSLSLTLQHFLPLAGNIVWPSDSSKPIIQFDPNDGGVSLIIAECDSDFNHVIENSPHEASLSRSFIHHLESSDSLASIMSLQITLFPNSGFCIGISTHHAVLDGKSSTMFVKAWAYLCKKAIEGDESQTLLSEFEPSFNREIVKDPKGNNVMDIVSTLFPSEKGNDRSLKIFPFEPKLEDSVRATFKLKHEDLDKIKQRVLSTWEIFDTKESKPQTLSSFVITCAYSLVCVAKAIHGAHNDKEKFSFVFSVDCRARLEPPIPNNYLGNCVWAYFVDTQPLDFIKEDGVFLVAKSIYEKTKMINEKGFLEGAINDMFEKLISLSSEGFEIMGVAGSHRFGVYEIDFGWGRPEKVEIVSIDRGLTIGLAESKDGRGGIEVGLVLNKPFWF