AlfalfaGEDB | MS.gene48584

M. sativa cultivar XinJiangDaYe / MS.gene48584

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016740 GO:0016746 GO:0016747
PFAM: PF02458.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48584.t1	XP_003621460.2	84.4	326	50	1	1	326	152	476	1.10E-156	562.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48584.t1	Q940Z5	39.5	329	179	11	1	323	153	467	4.4e-58	226.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48584.t1	G7L650	84.4	326	50	1	1	326	152	476	7.7e-157	562.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049232	MS.gene48584	0.855485	6.11E-62	-1.69E-46
MS.gene049396	MS.gene48584	0.81704	4.05E-52	-1.69E-46
MS.gene049633	MS.gene48584	0.806688	7.28E-50	-1.69E-46
MS.gene05010	MS.gene48584	0.804935	1.70E-49	-1.69E-46
MS.gene050170	MS.gene48584	0.849433	3.24E-60	-1.69E-46
MS.gene050216	MS.gene48584	0.820903	5.36E-53	-1.69E-46
MS.gene050323	MS.gene48584	0.862741	4.09E-64	-1.69E-46
MS.gene050496	MS.gene48584	0.839893	1.21E-57	-1.69E-46
MS.gene050662	MS.gene48584	0.800189	1.62E-48	-1.69E-46
MS.gene050697	MS.gene48584	0.841734	3.97E-58	-1.69E-46
MS.gene050868	MS.gene48584	0.850394	1.75E-60	-1.69E-46
MS.gene050871	MS.gene48584	0.852741	3.78E-61	-1.69E-46
MS.gene050904	MS.gene48584	0.806799	6.90E-50	-1.69E-46
MS.gene051268	MS.gene48584	0.816655	4.95E-52	-1.69E-46
MS.gene051874	MS.gene48584	0.802604	5.18E-49	-1.69E-46
MS.gene052066	MS.gene48584	0.843449	1.39E-58	-1.69E-46
MS.gene052966	MS.gene48584	0.812387	4.35E-51	-1.69E-46
MS.gene053633	MS.gene48584	0.883693	3.62E-71	-1.69E-46
MS.gene053824	MS.gene48584	0.811322	7.42E-51	-1.69E-46
MS.gene054003	MS.gene48584	0.804113	2.52E-49	-1.69E-46
MS.gene054595	MS.gene48584	0.810817	9.55E-51	-1.69E-46
MS.gene054954	MS.gene48584	0.809543	1.80E-50	-1.69E-46
MS.gene055750	MS.gene48584	0.804851	1.77E-49	-1.69E-46
MS.gene05589	MS.gene48584	0.819381	1.20E-52	-1.69E-46
MS.gene056046	MS.gene48584	0.850588	1.54E-60	-1.69E-46
MS.gene056387	MS.gene48584	0.859786	3.25E-63	-1.69E-46
MS.gene056504	MS.gene48584	0.844704	6.41E-59	-1.69E-46
MS.gene056510	MS.gene48584	0.820914	5.33E-53	-1.69E-46
MS.gene056618	MS.gene48584	-0.827183	1.80E-54	-1.69E-46
MS.gene057086	MS.gene48584	0.818799	1.62E-52	-1.69E-46
MS.gene057173	MS.gene48584	-0.802937	4.43E-49	-1.69E-46
MS.gene057857	MS.gene48584	0.810848	9.40E-51	-1.69E-46
MS.gene05787	MS.gene48584	0.805521	1.28E-49	-1.69E-46
MS.gene058157	MS.gene48584	0.813529	2.44E-51	-1.69E-46
MS.gene059367	MS.gene48584	0.815197	1.05E-51	-1.69E-46
MS.gene059699	MS.gene48584	-0.806866	6.68E-50	-1.69E-46
MS.gene059703	MS.gene48584	-0.819405	1.18E-52	-1.69E-46
MS.gene059865	MS.gene48584	0.844916	5.62E-59	-1.69E-46
MS.gene05995	MS.gene48584	0.816238	6.13E-52	-1.69E-46
MS.gene06000	MS.gene48584	0.84657	2.00E-59	-1.69E-46
MS.gene060595	MS.gene48584	-0.802518	5.40E-49	-1.69E-46
MS.gene060778	MS.gene48584	0.849605	2.91E-60	-1.69E-46
MS.gene06097	MS.gene48584	0.807617	4.63E-50	-1.69E-46
MS.gene060982	MS.gene48584	0.853037	3.11E-61	-1.69E-46
MS.gene061218	MS.gene48584	0.854819	9.55E-62	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene021914	MS.gene48584	PPI
MS.gene90496	MS.gene48584	PPI
MS.gene48584	MS.gene91596	PPI
MS.gene56795	MS.gene48584	PPI
MS.gene066657	MS.gene48584	PPI
MS.gene56798	MS.gene48584	PPI
MS.gene069423	MS.gene48584	PPI
MS.gene78818	MS.gene48584	PPI
MS.gene48584	MS.gene56799	PPI
MS.gene000763	MS.gene48584	PPI
MS.gene48584	MS.gene069767	PPI
MS.gene91615	MS.gene48584	PPI
MS.gene48584	MS.gene000763	PPI
MS.gene48584	MS.gene91613	PPI
MS.gene38977	MS.gene48584	PPI
MS.gene48584	MS.gene011716	PPI
MS.gene44633	MS.gene48584	PPI
MS.gene48584	MS.gene91595	PPI
MS.gene99223	MS.gene48584	PPI
MS.gene90492	MS.gene48584	PPI
MS.gene91614	MS.gene48584	PPI
MS.gene91612	MS.gene48584	PPI
MS.gene48584	MS.gene043352	PPI
MS.gene48585	MS.gene48584	PPI
MS.gene48584	MS.gene50384	PPI
MS.gene48584	MS.gene021909	PPI
MS.gene48584	MS.gene066658	PPI
MS.gene48584	MS.gene91612	PPI
MS.gene011837	MS.gene48584	PPI
MS.gene066634	MS.gene48584	PPI
MS.gene35741	MS.gene48584	PPI
MS.gene48584	MS.gene021917	PPI
MS.gene043346	MS.gene48584	PPI
MS.gene91599	MS.gene48584	PPI
MS.gene91598	MS.gene48584	PPI
MS.gene49853	MS.gene48584	PPI
MS.gene48584	MS.gene064101	PPI
MS.gene063865	MS.gene48584	PPI
MS.gene021910	MS.gene48584	PPI
MS.gene028996	MS.gene48584	PPI
MS.gene48584	MS.gene50356	PPI
MS.gene48584	MS.gene56797	PPI
MS.gene004218	MS.gene48584	PPI
MS.gene28251	MS.gene48584	PPI
MS.gene91613	MS.gene48584	PPI
MS.gene44644	MS.gene48584	PPI
MS.gene35745	MS.gene48584	PPI
MS.gene48584	MS.gene44630	PPI
MS.gene011796	MS.gene48584	PPI
MS.gene48584	MS.gene069422	PPI
MS.gene011801	MS.gene48584	PPI
MS.gene87537	MS.gene48584	PPI
MS.gene000764	MS.gene48584	PPI
MS.gene48582	MS.gene48584	PPI
MS.gene90493	MS.gene48584	PPI
MS.gene90491	MS.gene48584	PPI
MS.gene48584	MS.gene28251	PPI
MS.gene013787	MS.gene48584	PPI
MS.gene021906	MS.gene48584	PPI
MS.gene31542	MS.gene48584	PPI
MS.gene87532	MS.gene48584	PPI
MS.gene46265	MS.gene48584	PPI
MS.gene063868	MS.gene48584	PPI
MS.gene48584	MS.gene016532	PPI
MS.gene31540	MS.gene48584	PPI
MS.gene066658	MS.gene48584	PPI
MS.gene48581	MS.gene48584	PPI
MS.gene48584	MS.gene44647	PPI
MS.gene48584	MS.gene066701	PPI
MS.gene48584	MS.gene50355	PPI
MS.gene043348	MS.gene48584	PPI
MS.gene99221	MS.gene48584	PPI
MS.gene03202	MS.gene48584	PPI
MS.gene48584	MS.gene011799	PPI
MS.gene48584	MS.gene002544	PPI
MS.gene48584	MS.gene50420	PPI
MS.gene48584	MS.gene070270	PPI
MS.gene007831	MS.gene48584	PPI
MS.gene44642	MS.gene48584	PPI
MS.gene48584	MS.gene50382	PPI
MS.gene56796	MS.gene48584	PPI
MS.gene91609	MS.gene48584	PPI
MS.gene48584	MS.gene87536	PPI
MS.gene069767	MS.gene48584	PPI
MS.gene48584	MS.gene99218	PPI
MS.gene99222	MS.gene48584	PPI
MS.gene60208	MS.gene48584	PPI
MS.gene50355	MS.gene48584	PPI
MS.gene011798	MS.gene48584	PPI
MS.gene032349	MS.gene48584	PPI
MS.gene066656	MS.gene48584	PPI
MS.gene48584	MS.gene016477	PPI
MS.gene48584	MS.gene021913	PPI
MS.gene066660	MS.gene48584	PPI
MS.gene44640	MS.gene48584	PPI
MS.gene48584	MS.gene02573	PPI
MS.gene44463	MS.gene48584	PPI
MS.gene48584	MS.gene35740	PPI
MS.gene48584	MS.gene028994	PPI
MS.gene48584	MS.gene87577	PPI
MS.gene069422	MS.gene48584	PPI
MS.gene48584	MS.gene021905	PPI
MS.gene012360	MS.gene48584	PPI
MS.gene35744	MS.gene48584	PPI
MS.gene44643	MS.gene48584	PPI
MS.gene028999	MS.gene48584	PPI
MS.gene50379	MS.gene48584	PPI
MS.gene028998	MS.gene48584	PPI
MS.gene48584	MS.gene91614	PPI
MS.gene48584	MS.gene043350	PPI
MS.gene31545	MS.gene48584	PPI
MS.gene48584	MS.gene56798	PPI
MS.gene021915	MS.gene48584	PPI
MS.gene91611	MS.gene48584	PPI
MS.gene48584	MS.gene87191	PPI
MS.gene021904	MS.gene48584	PPI
MS.gene073299	MS.gene48584	PPI
MS.gene50382	MS.gene48584	PPI
MS.gene91597	MS.gene48584	PPI
MS.gene021916	MS.gene48584	PPI
MS.gene32261	MS.gene48584	PPI
MS.gene48584	MS.gene96337	PPI
MS.gene31546	MS.gene48584	PPI
MS.gene48584	MS.gene87496	PPI
MS.gene48584	MS.gene070019	PPI
MS.gene99219	MS.gene48584	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48584.t1	MTR_7g014160	84.356	326	50	1	1	326	152	476	0.0	563
MS.gene48584.t1	MTR_7g014610	76.147	327	76	2	2	326	152	478	0.0	516
MS.gene48584.t1	MTR_7g014200	70.336	327	88	3	1	326	153	471	2.48e-165	469
MS.gene48584.t1	MTR_7g014620	63.720	328	88	3	1	326	137	435	6.29e-139	400
MS.gene48584.t1	MTR_7g014670	96.237	186	7	0	141	326	77	262	1.90e-130	372
MS.gene48584.t1	MTR_7g013850	54.079	331	138	8	1	323	156	480	4.88e-115	341
MS.gene48584.t1	MTR_7g014630	86.395	147	20	0	1	147	153	299	6.40e-87	265
MS.gene48584.t1	MTR_7g014230	41.212	330	173	9	1	325	147	460	7.49e-73	232
MS.gene48584.t1	MTR_6g015315	38.154	325	182	7	1	323	145	452	3.81e-68	220
MS.gene48584.t1	MTR_7g014330	38.906	329	178	9	1	323	145	456	6.60e-66	214
MS.gene48584.t1	MTR_7g014250	38.415	328	181	8	1	323	145	456	9.58e-65	211
MS.gene48584.t1	MTR_6g015815	37.730	326	183	8	1	323	152	460	1.08e-63	209
MS.gene48584.t1	MTR_6g015850	36.585	328	181	8	1	323	152	457	9.12e-62	204
MS.gene48584.t1	MTR_6g015320	38.438	333	169	13	1	323	148	454	6.61e-61	201
MS.gene48584.t1	MTR_6g015830	36.000	325	189	8	1	323	153	460	1.50e-60	200
MS.gene48584.t1	MTR_7g014360	35.629	334	162	10	1	323	95	386	3.31e-59	195
MS.gene48584.t1	MTR_3g028210	34.954	329	190	8	1	323	154	464	6.27e-48	167
MS.gene48584.t1	MTR_3g087640	33.133	332	195	10	1	325	163	474	4.44e-42	152
MS.gene48584.t1	MTR_2g089765	32.845	341	188	10	1	323	165	482	2.39e-41	150
MS.gene48584.t1	MTR_6g015310	32.343	303	141	10	1	301	93	333	1.51e-39	142
MS.gene48584.t1	MTR_8g012750	30.588	340	195	8	1	321	153	470	1.32e-38	142
MS.gene48584.t1	MTR_2g089735	32.317	328	199	11	1	324	154	462	1.96e-38	142
MS.gene48584.t1	MTR_2g089670	29.483	329	203	6	1	323	172	477	1.52e-37	140
MS.gene48584.t1	MTR_2g089755	31.212	330	198	12	2	324	169	476	6.95e-34	130
MS.gene48584.t1	MTR_7g014310	29.967	307	148	8	1	306	66	306	1.47e-32	123
MS.gene48584.t1	MTR_2g089650	31.915	329	191	13	2	323	161	463	6.22e-31	121
MS.gene48584.t1	MTR_7g015860	28.483	323	188	10	1	309	153	446	6.74e-30	119
MS.gene48584.t1	MTR_7g014205	35.246	122	66	3	165	285	5	114	1.35e-15	72.4
MS.gene48584.t1	MTR_8g075610	24.169	331	202	11	1	321	128	419	3.71e-14	73.2
MS.gene48584.t1	MTR_3g026950	25.000	332	205	13	6	319	142	447	4.23e-14	73.2
MS.gene48584.t1	MTR_4g127650	23.410	346	205	10	1	323	148	456	3.08e-13	70.5
MS.gene48584.t1	MTR_4g022720	24.839	310	179	10	8	305	149	416	3.74e-12	67.0
MS.gene48584.t1	MTR_3g435290	45.588	68	35	1	257	324	53	118	1.11e-11	61.6
MS.gene48584.t1	MTR_4g022670	24.847	326	191	11	8	314	149	439	1.32e-11	65.5
MS.gene48584.t1	MTR_3g049150	25.284	352	196	16	1	324	136	448	4.72e-11	63.9
MS.gene48584.t1	MTR_3g026310	26.433	314	182	13	8	305	149	429	6.57e-11	63.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48584.t1	AT5G39050	39.394	330	180	11	1	324	153	468	1.33e-69	224
MS.gene48584.t1	AT5G39080	38.125	320	175	11	1	313	148	451	3.67e-63	207
MS.gene48584.t1	AT3G29670	36.810	326	180	7	1	324	149	450	1.60e-60	200
MS.gene48584.t1	AT3G29590	36.280	328	179	8	1	323	146	448	4.48e-59	196
MS.gene48584.t1	AT5G39090	34.146	328	183	12	1	323	148	447	7.47e-54	182
MS.gene48584.t1	AT3G29635	33.030	330	193	8	1	325	150	456	3.15e-51	176
MS.gene48584.t1	AT5G61160	31.056	322	197	7	1	319	147	446	2.88e-44	157
MS.gene48584.t1	AT3G29680	30.699	329	200	9	1	325	145	449	8.36e-43	153
MS.gene48584.t1	AT1G03940	30.233	344	183	12	1	323	157	464	2.12e-38	142
MS.gene48584.t1	AT5G23940	26.582	316	191	14	6	313	157	439	1.99e-17	83.2
MS.gene48584.t1	AT3G29690	28.931	159	92	4	173	323	33	178	7.05e-16	75.1
MS.gene48584.t1	AT5G41040	24.925	333	194	12	1	321	152	440	1.53e-15	77.4
MS.gene48584.t1	AT5G41040	24.925	333	194	12	1	321	168	456	1.58e-15	77.4
MS.gene48584.t1	AT5G57840	24.706	340	207	14	1	326	137	441	5.49e-15	75.9

Find 69 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCACTACCAAATAACTATTT+TGG	0.135502	7.2:-83066790	MS.gene48584:CDS
AATAACCACTTTCTGGAAAA+AGG	0.147614	7.2:+83067340	None:intergenic
CCAATGGAATAACCACTTTC+TGG	0.167311	7.2:+83067333	None:intergenic
AGCAAATGGTGTTATCAAAA+TGG	0.193782	7.2:-83066984	MS.gene48584:CDS
GTGATAAGTTCACAAATAAT+TGG	0.223223	7.2:-83067143	MS.gene48584:CDS
GTTATCAAAATGGGAATTAT+TGG	0.234732	7.2:-83066974	MS.gene48584:CDS
GGTGTTTATGAGATTGATTT+TGG	0.265782	7.2:-83066556	MS.gene48584:CDS
CAATTTCCAAAATAGTTATT+TGG	0.276645	7.2:+83066784	None:intergenic
AGCAATTCATGGAATTAAAA+TGG	0.285041	7.2:-83066869	MS.gene48584:CDS
AATTACAACCGTTGTTTGAT+TGG	0.287075	7.2:-83067194	MS.gene48584:CDS
TGCTTATGCCCTTGTTTGTT+TGG	0.306724	7.2:-83066896	MS.gene48584:CDS
CGAAGAGAGTCTTCGAGCTT+TGG	0.323617	7.2:+83067048	None:intergenic
AAGATGTTCCCAAATGAAAA+AGG	0.331198	7.2:-83067105	MS.gene48584:CDS
GAGAAAGAGAAGTTTGCATT+TGG	0.340510	7.2:-83066847	MS.gene48584:CDS
AACTTATCACCAAGTTCATC+TGG	0.356756	7.2:+83067156	None:intergenic
TTTAGAACGTTGTTTCTTGA+AGG	0.361727	7.2:-83066403	MS.gene48584:CDS
CAACAAAGGTTGTGGTTCTT+CGG	0.369447	7.2:+83067221	None:intergenic
CAACCATGTTTATCAAAGCT+TGG	0.376272	7.2:-83067272	MS.gene48584:CDS
GAAACTATCTCCACCTTTGT+TGG	0.379376	7.2:+83066523	None:intergenic
GTGGATAGAGGTTTAACAAT+TGG	0.394688	7.2:-83066499	MS.gene48584:CDS
AGTGAAGGATTTGAAATAAT+AGG	0.395317	7.2:-83066601	MS.gene48584:CDS
ACTCACCATGCTGTTCTTGA+TGG	0.395647	7.2:-83067303	MS.gene48584:CDS
AAATTGTGTGTGGGGACATT+TGG	0.398292	7.2:-83066767	MS.gene48584:CDS
AACCATGTTTATCAAAGCTT+GGG	0.399105	7.2:-83067271	MS.gene48584:CDS
TACCATCTTTGCTCTCTGCC+AGG	0.406929	7.2:+83066476	None:intergenic
TGTTTGGCAAAAGCAATTCA+TGG	0.416726	7.2:-83066880	MS.gene48584:CDS
GCAAATGGTGTTATCAAAAT+GGG	0.418223	7.2:-83066983	MS.gene48584:CDS
GTGGCGGGATCTAATAGATT+TGG	0.423530	7.2:-83066577	MS.gene48584:CDS
CCAGAAAGTGGTTATTCCAT+TGG	0.423707	7.2:-83067333	MS.gene48584:CDS
AATAAAGATGATAAATGAAA+AGG	0.424032	7.2:-83066674	MS.gene48584:CDS
GGTAAAGGTGGAATTGAAGT+TGG	0.427876	7.2:-83066457	MS.gene48584:CDS
GAGATAGTTTCGGTGGATAG+AGG	0.442745	7.2:-83066511	MS.gene48584:CDS
TACAATTATTTGTCAAGTGA+AGG	0.447336	7.2:-83066616	MS.gene48584:CDS
ATAAAGATGATAAATGAAAA+GGG	0.447547	7.2:-83066673	MS.gene48584:CDS
AATGAAAAGGGTGTTCTTGA+AGG	0.453937	7.2:-83066661	MS.gene48584:CDS
TTGGATTACATAAATGAAGA+TGG	0.466086	7.2:-83066730	MS.gene48584:CDS
GTAAAGGTGGAATTGAAGTT+GGG	0.475472	7.2:-83066456	MS.gene48584:CDS
GAGAACAAACGATGATAAAG+TGG	0.484849	7.2:+83066924	None:intergenic
TTTGAACAAGATAAAGCAAA+TGG	0.487133	7.2:-83066998	MS.gene48584:CDS
GTTCTTGAAGGAGCTAGTGA+TGG	0.493087	7.2:-83066649	MS.gene48584:CDS
TTCTTGAAGGAGCTAGTGAT+GGG	0.497785	7.2:-83066648	MS.gene48584:CDS
AACAAAGGTGGAGATAGTTT+CGG	0.498546	7.2:-83066521	MS.gene48584:CDS
AATTCTTGCAACAAAGGTTG+TGG	0.499343	7.2:+83067213	None:intergenic
GGTTGTAATTCTTGCAACAA+AGG	0.507240	7.2:+83067207	None:intergenic
TTTGGTTGATACAAAACCGT+TGG	0.511791	7.2:-83066749	MS.gene48584:CDS
TGGATTACATAAATGAAGAT+GGG	0.512542	7.2:-83066729	MS.gene48584:CDS
TAGAGGTTTAACAATTGGCC+TGG	0.518320	7.2:-83066494	MS.gene48584:CDS
AAATAGTTATTTGGTAGTGA+TGG	0.521146	7.2:+83066793	None:intergenic
GTCAAAGACCCAGATGAACT+TGG	0.526008	7.2:-83067165	MS.gene48584:CDS
TGTTCTAGATAAGCATGTAA+TGG	0.534624	7.2:-83066431	MS.gene48584:CDS
GGCCTGGCAGAGAGCAAAGA+TGG	0.534800	7.2:-83066478	MS.gene48584:CDS
TAACCTTGCTCTGCAATCTA+CGG	0.549902	7.2:+83066819	None:intergenic
TCTTCATTTATGTAATCCAA+CGG	0.557148	7.2:+83066733	None:intergenic
TTTGAAATAATAGGAGTGGC+GGG	0.565832	7.2:-83066592	MS.gene48584:CDS
AGCCCAAGCTTTGATAAACA+TGG	0.576503	7.2:+83067269	None:intergenic
ATTTGAAATAATAGGAGTGG+CGG	0.578943	7.2:-83066593	MS.gene48584:CDS
GCAGAGAGCAAAGATGGTAA+AGG	0.579752	7.2:-83066472	MS.gene48584:CDS
ACTTATCACCAAGTTCATCT+GGG	0.602703	7.2:+83067157	None:intergenic
ATGGTGAGTGCTTATTCCAA+TGG	0.605931	7.2:+83067317	None:intergenic
TCACCGTAGATTGCAGAGCA+AGG	0.607885	7.2:-83066822	MS.gene48584:CDS
GTGGGGAAGACCAACAAAGG+TGG	0.612550	7.2:-83066533	MS.gene48584:CDS
GAGAGCAAAGATGGTAAAGG+TGG	0.619484	7.2:-83066469	MS.gene48584:CDS
AGGATTTGAAATAATAGGAG+TGG	0.624166	7.2:-83066596	MS.gene48584:CDS
TGGGTGGGGAAGACCAACAA+AGG	0.627130	7.2:-83066536	MS.gene48584:CDS
AAAGGTGGAGATAGTTTCGG+TGG	0.628419	7.2:-83066518	MS.gene48584:CDS
ACGATATTCCAATCAAACAA+CGG	0.632005	7.2:+83067186	None:intergenic
GGATTACATAAATGAAGATG+GGG	0.640685	7.2:-83066728	MS.gene48584:CDS
ACAAACGATGATAAAGTGGG+TGG	0.683948	7.2:+83066928	None:intergenic
AGAACAAACGATGATAAAGT+GGG	0.738379	7.2:+83066925	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATAAAGATGATAAATGAAA+AGG	-	chr7.2:83067058-83067077	MS.gene48584:CDS	15.0%
!!	ATAAAGATGATAAATGAAAA+GGG	-	chr7.2:83067059-83067078	MS.gene48584:CDS	15.0%
!!	CAATTTCCAAAATAGTTATT+TGG	+	chr7.2:83066951-83066970	None:intergenic	20.0%
!	AGCAATTCATGGAATTAAAA+TGG	-	chr7.2:83066863-83066882	MS.gene48584:CDS	25.0%
!	GTGATAAGTTCACAAATAAT+TGG	-	chr7.2:83066589-83066608	MS.gene48584:CDS	25.0%
!	GTTATCAAAATGGGAATTAT+TGG	-	chr7.2:83066758-83066777	MS.gene48584:CDS	25.0%
!	TACAATTATTTGTCAAGTGA+AGG	-	chr7.2:83067116-83067135	MS.gene48584:CDS	25.0%
!	TCTTCATTTATGTAATCCAA+CGG	+	chr7.2:83067002-83067021	None:intergenic	25.0%
!	TGGATTACATAAATGAAGAT+GGG	-	chr7.2:83067003-83067022	MS.gene48584:CDS	25.0%
!	TTGGATTACATAAATGAAGA+TGG	-	chr7.2:83067002-83067021	MS.gene48584:CDS	25.0%
!	TTTGAACAAGATAAAGCAAA+TGG	-	chr7.2:83066734-83066753	MS.gene48584:CDS	25.0%
!!	AGTGAAGGATTTGAAATAAT+AGG	-	chr7.2:83067131-83067150	MS.gene48584:CDS	25.0%
!!	TCACTACCAAATAACTATTT+TGG	-	chr7.2:83066942-83066961	MS.gene48584:CDS	25.0%
!!!	AAATAGTTATTTGGTAGTGA+TGG	+	chr7.2:83066942-83066961	None:intergenic	25.0%
!!!	GTGTTTATGAGATTGATTTT+GGG	-	chr7.2:83067177-83067196	MS.gene48584:CDS	25.0%
!!!	TTTCGTTTCCTTTTTCATTT+GGG	+	chr7.2:83066638-83066657	None:intergenic	25.0%
	AACCATGTTTATCAAAGCTT+GGG	-	chr7.2:83066461-83066480	MS.gene48584:CDS	30.0%
	AAGATGTTCCCAAATGAAAA+AGG	-	chr7.2:83066627-83066646	MS.gene48584:CDS	30.0%
	AATTACAACCGTTGTTTGAT+TGG	-	chr7.2:83066538-83066557	MS.gene48584:CDS	30.0%
	ACGATATTCCAATCAAACAA+CGG	+	chr7.2:83066549-83066568	None:intergenic	30.0%
	AGAACAAACGATGATAAAGT+GGG	+	chr7.2:83066810-83066829	None:intergenic	30.0%
	GGATTACATAAATGAAGATG+GGG	-	chr7.2:83067004-83067023	MS.gene48584:CDS	30.0%
!	AATAACCACTTTCTGGAAAA+AGG	+	chr7.2:83066395-83066414	None:intergenic	30.0%
!	AGGATTTGAAATAATAGGAG+TGG	-	chr7.2:83067136-83067155	MS.gene48584:CDS	30.0%
!	ATAACCACTTTCTGGAAAAA+GGG	+	chr7.2:83066394-83066413	None:intergenic	30.0%
!	ATTTGAAATAATAGGAGTGG+CGG	-	chr7.2:83067139-83067158	MS.gene48584:CDS	30.0%
!	TGTTCTAGATAAGCATGTAA+TGG	-	chr7.2:83067301-83067320	MS.gene48584:CDS	30.0%
!!	ACTATTTTGGAAATTGTGTG+TGG	-	chr7.2:83066955-83066974	MS.gene48584:CDS	30.0%
!!	AGCAAATGGTGTTATCAAAA+TGG	-	chr7.2:83066748-83066767	MS.gene48584:CDS	30.0%
!!	CTATTTTGGAAATTGTGTGT+GGG	-	chr7.2:83066956-83066975	MS.gene48584:CDS	30.0%
!!	CTTTCGTTTCCTTTTTCATT+TGG	+	chr7.2:83066639-83066658	None:intergenic	30.0%
!!	GCAAATGGTGTTATCAAAAT+GGG	-	chr7.2:83066749-83066768	MS.gene48584:CDS	30.0%
!!	GGTGTTTATGAGATTGATTT+TGG	-	chr7.2:83067176-83067195	MS.gene48584:CDS	30.0%
!!	TATTTTGGAAATTGTGTGTG+GGG	-	chr7.2:83066957-83066976	MS.gene48584:CDS	30.0%
!!	TTATGAGATTGATTTTGGGT+GGG	-	chr7.2:83067181-83067200	MS.gene48584:CDS	30.0%
!!	TTTAGAACGTTGTTTCTTGA+AGG	-	chr7.2:83067329-83067348	MS.gene48584:CDS	30.0%
!!	TTTATGAGATTGATTTTGGG+TGG	-	chr7.2:83067180-83067199	MS.gene48584:CDS	30.0%
	AACAAAGGTGGAGATAGTTT+CGG	-	chr7.2:83067211-83067230	MS.gene48584:CDS	35.0%
	AACTTATCACCAAGTTCATC+TGG	+	chr7.2:83066579-83066598	None:intergenic	35.0%
	AATTCTTGCAACAAAGGTTG+TGG	+	chr7.2:83066522-83066541	None:intergenic	35.0%
	ACTTATCACCAAGTTCATCT+GGG	+	chr7.2:83066578-83066597	None:intergenic	35.0%
	CAACCATGTTTATCAAAGCT+TGG	-	chr7.2:83066460-83066479	MS.gene48584:CDS	35.0%
	GAGAAAGAGAAGTTTGCATT+TGG	-	chr7.2:83066885-83066904	MS.gene48584:CDS	35.0%
	GAGAACAAACGATGATAAAG+TGG	+	chr7.2:83066811-83066830	None:intergenic	35.0%
	GGTTGTAATTCTTGCAACAA+AGG	+	chr7.2:83066528-83066547	None:intergenic	35.0%
	GTAAAGGTGGAATTGAAGTT+GGG	-	chr7.2:83067276-83067295	MS.gene48584:CDS	35.0%
	GTGGATAGAGGTTTAACAAT+TGG	-	chr7.2:83067233-83067252	MS.gene48584:CDS	35.0%
	TGTTTGGCAAAAGCAATTCA+TGG	-	chr7.2:83066852-83066871	MS.gene48584:CDS	35.0%
!	ATAACCCTTTTTCCAGAAAG+TGG	-	chr7.2:83066387-83066406	MS.gene48584:CDS	35.0%
!	ATTTTCCATCAAGAACAGCA+TGG	+	chr7.2:83066437-83066456	None:intergenic	35.0%
!	TGCTTTTGCCAAACAAACAA+GGG	+	chr7.2:83066847-83066866	None:intergenic	35.0%
!	TTTGAAATAATAGGAGTGGC+GGG	-	chr7.2:83067140-83067159	MS.gene48584:CDS	35.0%
!	TTTGGTTGATACAAAACCGT+TGG	-	chr7.2:83066983-83067002	MS.gene48584:CDS	35.0%
!!	AATGAAAAGGGTGTTCTTGA+AGG	-	chr7.2:83067071-83067090	MS.gene48584:CDS	35.0%
!!	TATGAGATTGATTTTGGGTG+GGG	-	chr7.2:83067182-83067201	MS.gene48584:CDS	35.0%
!!	TTGCTTTTGCCAAACAAACA+AGG	+	chr7.2:83066848-83066867	None:intergenic	35.0%
	AAATTGTGTGTGGGGACATT+TGG	-	chr7.2:83066965-83066984	MS.gene48584:CDS	40.0%
	ACAAACGATGATAAAGTGGG+TGG	+	chr7.2:83066807-83066826	None:intergenic	40.0%
	ATGGTGAGTGCTTATTCCAA+TGG	+	chr7.2:83066418-83066437	None:intergenic	40.0%
	CAACAAAGGTTGTGGTTCTT+CGG	+	chr7.2:83066514-83066533	None:intergenic	40.0%
	CCAATGGAATAACCACTTTC+TGG	+	chr7.2:83066402-83066421	None:intergenic	40.0%
	CCAGAAAGTGGTTATTCCAT+TGG	-	chr7.2:83066399-83066418	MS.gene48584:CDS	40.0%
	GAAACTATCTCCACCTTTGT+TGG	+	chr7.2:83067212-83067231	None:intergenic	40.0%
	GGTAAAGGTGGAATTGAAGT+TGG	-	chr7.2:83067275-83067294	MS.gene48584:CDS	40.0%
	TAACCTTGCTCTGCAATCTA+CGG	+	chr7.2:83066916-83066935	None:intergenic	40.0%
	TAGAGGTTTAACAATTGGCC+TGG	-	chr7.2:83067238-83067257	MS.gene48584:CDS	40.0%
	TTCTTGAAGGAGCTAGTGAT+GGG	-	chr7.2:83067084-83067103	MS.gene48584:CDS	40.0%
!	AGCCCAAGCTTTGATAAACA+TGG	+	chr7.2:83066466-83066485	None:intergenic	40.0%
!	TGCTTATGCCCTTGTTTGTT+TGG	-	chr7.2:83066836-83066855	MS.gene48584:CDS	40.0%
	AAAGGTGGAGATAGTTTCGG+TGG	-	chr7.2:83067214-83067233	MS.gene48584:CDS	45.0%
	ACTCACCATGCTGTTCTTGA+TGG	-	chr7.2:83066429-83066448	MS.gene48584:CDS	45.0%
	GAGAGCAAAGATGGTAAAGG+TGG	-	chr7.2:83067263-83067282	MS.gene48584:CDS	45.0%
	GAGATAGTTTCGGTGGATAG+AGG	-	chr7.2:83067221-83067240	MS.gene48584:CDS	45.0%
	GCAGAGAGCAAAGATGGTAA+AGG	-	chr7.2:83067260-83067279	MS.gene48584:CDS	45.0%
	GTCAAAGACCCAGATGAACT+TGG	-	chr7.2:83066567-83066586	MS.gene48584:CDS	45.0%
	GTTCTTGAAGGAGCTAGTGA+TGG	-	chr7.2:83067083-83067102	MS.gene48584:CDS	45.0%
!	GTGGCGGGATCTAATAGATT+TGG	-	chr7.2:83067155-83067174	MS.gene48584:CDS	45.0%
	CGAAGAGAGTCTTCGAGCTT+TGG	+	chr7.2:83066687-83066706	None:intergenic	50.0%
	TACCATCTTTGCTCTCTGCC+AGG	+	chr7.2:83067259-83067278	None:intergenic	50.0%
	TCACCGTAGATTGCAGAGCA+AGG	-	chr7.2:83066910-83066929	MS.gene48584:CDS	50.0%
	GTGGGGAAGACCAACAAAGG+TGG	-	chr7.2:83067199-83067218	MS.gene48584:CDS	55.0%
	TGGGTGGGGAAGACCAACAA+AGG	-	chr7.2:83067196-83067215	MS.gene48584:CDS	55.0%
	GGCCTGGCAGAGAGCAAAGA+TGG	-	chr7.2:83067254-83067273	MS.gene48584:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	83066387	83067367	83066387	ID=MS.gene48584
chr7.2	mRNA	83066387	83067367	83066387	ID=MS.gene48584.t1;Parent=MS.gene48584
chr7.2	exon	83066387	83067367	83066387	ID=MS.gene48584.t1.exon1;Parent=MS.gene48584.t1
chr7.2	CDS	83066387	83067367	83066387	ID=cds.MS.gene48584.t1;Parent=MS.gene48584.t1

Gene Sequence

>MS.gene48584

ATAACCCTTTTTCCAGAAAGTGGTTATTCCATTGGAATAAGCACTCACCATGCTGTTCTTGATGGAAAATCTTCAACCATGTTTATCAAAGCTTGGGCTTATCTATGCAACAAAAACATTGAAACCGAAGAACCACAACCTTTGTTGCAAGAATTACAACCGTTGTTTGATTGGAATATCGTCAAAGACCCAGATGAACTTGGTGATAAGTTCACAAATAATTGGAGAGAGATCGTTACTAAGATGTTCCCAAATGAAAAAGGAAACGAAAGATGCTTGAAGATTTTAGCTTTTGAACCAAAGCTCGAAGACTCTCTTCGTGCTACGTTCAAGCTCACACGTGAAGATTTGAACAAGATAAAGCAAATGGTGTTATCAAAATGGGAATTATTGGACACAAATGAATTAAATTCTAAGCCACCCACTTTATCATCGTTTGTTCTCACATGTGCTTATGCCCTTGTTTGTTTGGCAAAAGCAATTCATGGAATTAAAATGGAGAAAGAGAAGTTTGCATTTGGATTCACCGTAGATTGCAGAGCAAGGTTAGAGCCATCACTACCAAATAACTATTTTGGAAATTGTGTGTGGGGACATTTGGTTGATACAAAACCGTTGGATTACATAAATGAAGATGGGGTGTTTTTAGTTGCTAAGTGTATTCATGAGAAAATAAAGATGATAAATGAAAAGGGTGTTCTTGAAGGAGCTAGTGATGGGTTTAGTAAATACAATTATTTGTCAAGTGAAGGATTTGAAATAATAGGAGTGGCGGGATCTAATAGATTTGGTGTTTATGAGATTGATTTTGGGTGGGGAAGACCAACAAAGGTGGAGATAGTTTCGGTGGATAGAGGTTTAACAATTGGCCTGGCAGAGAGCAAAGATGGTAAAGGTGGAATTGAAGTTGGGCTTGTTCTAGATAAGCATGTAATGGATCTCTTTAGAACGTTGTTTCTTGAAGGACTTCACATTAATTGA

Protein sequence

>MS.gene48584.t1

ITLFPESGYSIGISTHHAVLDGKSSTMFIKAWAYLCNKNIETEEPQPLLQELQPLFDWNIVKDPDELGDKFTNNWREIVTKMFPNEKGNERCLKILAFEPKLEDSLRATFKLTREDLNKIKQMVLSKWELLDTNELNSKPPTLSSFVLTCAYALVCLAKAIHGIKMEKEKFAFGFTVDCRARLEPSLPNNYFGNCVWGHLVDTKPLDYINEDGVFLVAKCIHEKIKMINEKGVLEGASDGFSKYNYLSSEGFEIIGVAGSNRFGVYEIDFGWGRPTKVEIVSVDRGLTIGLAESKDGKGGIEVGLVLDKHVMDLFRTLFLEGLHIN