AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene91611

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91611.t1 MTR_7g013850 77.226 483 107 2 1 480 1 483 0.0 766
MS.gene91611.t1 MTR_7g014200 59.213 483 176 12 1 477 1 468 0.0 546
MS.gene91611.t1 MTR_7g014160 57.380 481 190 10 5 480 6 476 0.0 537
MS.gene91611.t1 MTR_7g014610 58.351 485 186 12 1 478 1 476 0.0 516
MS.gene91611.t1 MTR_7g014620 51.828 465 186 9 19 480 6 435 1.35e-150 436
MS.gene91611.t1 MTR_7g014630 49.721 358 161 7 6 350 7 358 6.34e-107 322
MS.gene91611.t1 MTR_6g015320 39.709 481 259 10 1 477 1 454 3.49e-106 323
MS.gene91611.t1 MTR_6g015315 39.789 475 261 10 4 477 2 452 2.01e-105 322
MS.gene91611.t1 MTR_6g015850 40.491 489 247 16 1 477 1 457 2.80e-101 311
MS.gene91611.t1 MTR_6g015815 41.826 471 240 14 11 477 20 460 6.50e-101 310
MS.gene91611.t1 MTR_6g015830 40.041 482 246 13 9 477 9 460 3.45e-99 306
MS.gene91611.t1 MTR_7g014330 37.838 481 260 15 9 477 3 456 3.35e-98 303
MS.gene91611.t1 MTR_7g014230 37.526 477 265 11 9 476 5 457 1.98e-97 301
MS.gene91611.t1 MTR_7g014250 37.578 479 264 14 9 477 3 456 5.26e-97 300
MS.gene91611.t1 MTR_3g028210 35.818 483 271 14 8 477 8 464 8.61e-81 258
MS.gene91611.t1 MTR_7g014180 68.156 179 50 5 1 178 1 173 2.10e-73 229
MS.gene91611.t1 MTR_2g089765 32.724 492 276 15 11 477 21 482 2.99e-73 239
MS.gene91611.t1 MTR_7g014360 32.518 409 226 12 76 477 21 386 2.07e-64 213
MS.gene91611.t1 MTR_7g014670 54.211 190 85 2 289 477 71 259 6.06e-64 208
MS.gene91611.t1 MTR_2g089650 30.309 485 293 12 8 477 9 463 1.30e-63 214
MS.gene91611.t1 MTR_3g087640 31.772 491 285 16 9 477 10 472 2.40e-63 213
MS.gene91611.t1 MTR_2g089670 32.051 468 269 14 26 477 43 477 2.54e-63 213
MS.gene91611.t1 MTR_7g015860 31.959 485 258 15 8 463 5 446 1.11e-62 211
MS.gene91611.t1 MTR_2g089755 31.197 468 275 14 28 477 37 475 1.88e-60 206
MS.gene91611.t1 MTR_2g089735 31.920 448 269 12 41 477 39 461 2.48e-59 202
MS.gene91611.t1 MTR_8g012750 31.343 469 281 16 26 475 24 470 1.58e-57 197
MS.gene91611.t1 MTR_6g015310 32.099 405 202 11 51 455 2 333 4.25e-53 182
MS.gene91611.t1 MTR_7g014310 27.027 333 174 8 130 460 41 306 3.33e-33 128
MS.gene91611.t1 MTR_2g089665 26.211 454 200 15 11 454 7 335 3.58e-29 117
MS.gene91611.t1 MTR_3g435300 32.906 234 132 7 7 229 3 222 2.60e-28 113
MS.gene91611.t1 MTR_3g101900 25.000 452 279 16 28 466 30 434 1.59e-23 103
MS.gene91611.t1 MTR_3g070170 25.054 467 265 21 8 439 12 428 1.65e-21 97.4
MS.gene91611.t1 MTR_4g007540 24.153 443 256 17 8 429 3 386 3.79e-20 93.2
MS.gene91611.t1 MTR_8g075610 23.936 376 225 12 67 429 47 374 4.58e-20 92.8
MS.gene91611.t1 MTR_3g026310 22.737 453 267 16 17 441 14 411 1.88e-18 88.2
MS.gene91611.t1 MTR_5g081960 23.701 481 321 17 13 466 11 472 2.64e-18 87.8
MS.gene91611.t1 MTR_6g045553 24.348 460 282 16 7 439 13 433 3.52e-18 87.4
MS.gene91611.t1 MTR_7g086000 23.980 392 226 13 67 429 60 408 5.27e-18 86.7
MS.gene91611.t1 MTR_3g101930 24.895 474 292 19 13 466 12 441 6.02e-18 86.7
MS.gene91611.t1 MTR_4g094842 24.265 408 222 15 62 439 57 407 1.61e-17 85.1
MS.gene91611.t1 MTR_3g026950 24.658 438 261 18 64 473 51 447 1.81e-17 85.1
MS.gene91611.t1 MTR_3g102020 24.318 440 279 18 13 439 11 409 1.93e-17 85.1
MS.gene91611.t1 MTR_2g007880 24.242 462 240 20 50 470 48 440 5.46e-17 83.6
MS.gene91611.t1 MTR_3g049150 23.992 496 293 20 8 473 2 443 2.51e-16 81.6
MS.gene91611.t1 MTR_2g064495 22.318 466 280 19 31 475 27 431 7.19e-16 80.1
MS.gene91611.t1 MTR_7g014210 34.579 107 68 2 335 439 24 130 7.29e-16 75.5
MS.gene91611.t1 MTR_2g105330 22.318 466 280 19 31 475 27 431 1.95e-15 78.6
MS.gene91611.t1 MTR_3g102010 22.297 444 292 15 8 439 7 409 2.92e-15 78.2
MS.gene91611.t1 MTR_8g467580 24.481 482 260 22 17 467 15 423 3.17e-15 78.2
MS.gene91611.t1 MTR_4g022720 24.944 445 254 19 17 441 14 398 3.26e-15 78.2
MS.gene91611.t1 MTR_0190s0020 24.766 428 257 15 25 439 26 401 1.66e-14 75.9
MS.gene91611.t1 MTR_8g096530 24.330 448 243 15 7 429 6 382 5.15e-14 74.3
MS.gene91611.t1 MTR_2g015430 21.663 457 290 14 8 456 2 398 6.53e-14 73.9
MS.gene91611.t1 MTR_7g085930 24.221 417 258 17 36 429 34 415 1.02e-13 73.6
MS.gene91611.t1 MTR_4g015180 22.453 481 334 15 10 466 8 473 1.04e-13 73.6
MS.gene91611.t1 MTR_8g467590 25.728 412 235 20 44 442 41 394 1.95e-13 72.4
MS.gene91611.t1 MTR_7g014340 54.930 71 25 2 9 75 3 70 6.44e-13 65.1
MS.gene91611.t1 MTR_7g014205 28.226 124 77 2 317 439 2 114 7.75e-13 65.5
MS.gene91611.t1 MTR_4g022670 24.115 452 257 17 17 442 14 405 1.38e-12 69.7
MS.gene91611.t1 MTR_7g084940 23.037 382 229 12 61 429 63 392 1.54e-12 69.7
MS.gene91611.t1 MTR_4g127650 21.145 454 297 14 41 475 43 454 2.53e-12 69.3
MS.gene91611.t1 MTR_4g097090 23.877 423 258 19 26 428 28 406 5.18e-12 68.2
MS.gene91611.t1 MTR_7g014220 60.377 53 18 1 25 77 15 64 5.67e-12 62.0
MS.gene91611.t1 MTR_8g079980 23.831 449 271 18 17 453 15 404 2.03e-11 66.2
MS.gene91611.t1 MTR_4g022760 25.000 356 223 14 17 369 14 328 3.09e-11 65.5
MS.gene91611.t1 MTR_3g435290 35.246 122 72 3 358 477 1 117 3.40e-11 60.8
MS.gene91611.t1 MTR_8g041770 22.449 441 281 17 7 439 13 400 7.02e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91611.t1 AT3G29635 38.950 457 249 11 24 477 25 454 3.65e-92 288
MS.gene91611.t1 AT3G29590 37.074 499 241 12 1 477 1 448 2.71e-91 285
MS.gene91611.t1 AT5G39050 37.787 479 268 14 8 477 10 467 3.82e-88 278
MS.gene91611.t1 AT5G39080 37.764 474 265 15 1 467 1 451 1.73e-86 273
MS.gene91611.t1 AT3G29670 37.161 479 260 14 8 477 3 449 4.97e-86 271
MS.gene91611.t1 AT5G39090 36.250 480 270 12 1 477 1 447 1.79e-84 267
MS.gene91611.t1 AT5G61160 34.820 471 275 13 8 473 3 446 9.42e-81 258
MS.gene91611.t1 AT3G29680 36.820 478 258 14 4 473 2 443 8.73e-78 250
MS.gene91611.t1 AT1G03940 29.800 500 292 13 1 477 1 464 3.10e-65 218
MS.gene91611.t1 AT1G03495 30.728 371 216 12 1 359 1 342 6.30e-42 152
MS.gene91611.t1 AT5G23940 24.242 462 266 20 8 439 3 410 6.06e-21 95.9
MS.gene91611.t1 AT3G29720 35.616 219 100 10 99 303 10 201 1.88e-20 90.1
MS.gene91611.t1 AT3G50280 23.529 425 271 16 19 434 18 397 5.39e-20 92.8
MS.gene91611.t1 AT5G07850 25.503 447 279 19 1 434 1 406 2.08e-16 82.0
MS.gene91611.t1 AT3G29690 31.126 151 98 2 328 477 33 178 3.84e-16 77.0
MS.gene91611.t1 AT5G48930 22.292 480 311 18 8 475 3 432 9.23e-16 79.7
MS.gene91611.t1 AT4G31910 23.077 364 242 11 44 403 46 375 5.41e-13 71.2
MS.gene91611.t1 AT3G50280 23.920 301 185 13 141 434 4 267 6.57e-13 70.1
MS.gene91611.t1 AT2G40230 23.341 437 285 19 8 439 5 396 2.88e-12 68.9
MS.gene91611.t1 AT3G47170 24.138 348 215 12 51 388 61 369 5.84e-12 68.2
MS.gene91611.t1 AT5G41040 22.193 374 244 16 61 429 63 394 1.26e-11 67.0
MS.gene91611.t1 AT5G41040 22.667 375 241 17 61 429 79 410 1.60e-11 66.6
MS.gene91611.t1 AT2G25150 22.717 427 264 18 64 469 64 445 1.86e-11 66.6
MS.gene91611.t1 AT3G48720 24.399 291 173 8 67 352 61 309 6.56e-11 64.7

Find 63 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTACTTCCCTACTCAAAA+AGG 0.196644 7.4:+85113875 None:intergenic
GTAGGGTGTGATTGTGAATT+TGG 0.209360 7.4:+85114350 None:intergenic
GAGAGTGAAGATTTGAGTTT+TGG 0.216722 7.4:+85114305 None:intergenic
GATAATTGGTTTGCAGAATT+AGG 0.230849 7.4:+85114446 None:intergenic
TTGAAAGGTGGTGTTGAATT+TGG 0.233566 7.4:-85113147 MS.gene91611:CDS
ACAAAAGTTGACAATTTCTT+TGG 0.263553 7.4:+85113618 None:intergenic
AACGAAACTCCGTCATCTTT+TGG 0.264453 7.4:+85114200 None:intergenic
GGAGTATATGAGAATGATTT+TGG 0.273264 7.4:-85113246 MS.gene91611:CDS
AAGTGAGAGGAAGAGATAAT+TGG 0.284573 7.4:+85114432 None:intergenic
CGAACGGAATCCTTAACTTT+TGG 0.284718 7.4:+85113741 None:intergenic
TTCTTCAATATTGTTCCATT+TGG 0.285958 7.4:+85113662 None:intergenic
TACATTGTTGTCTAGATATA+TGG 0.286798 7.4:-85113322 MS.gene91611:CDS
CGAGCTCACACGTGAAGATT+TGG 0.319044 7.4:-85113709 MS.gene91611:CDS
CTATAGCCGGTAACATTGTT+TGG 0.330391 7.4:-85114259 MS.gene91611:CDS
CCAATTCCCGAAAACTATTT+CGG 0.342065 7.4:-85113477 MS.gene91611:CDS
TCACTTTCTTAGACTTGAAT+TGG 0.344769 7.4:-85114415 MS.gene91611:CDS
ACACATCATGTTGTTCTTGA+TGG 0.353250 7.4:-85113993 MS.gene91611:CDS
TTGAACTGGATGATAGGTTT+TGG 0.361338 7.4:+85114224 None:intergenic
TTTGGATTGAACTGGATGAT+AGG 0.371297 7.4:+85114218 None:intergenic
TTCAAAAGATTTACATATAG+AGG 0.378686 7.4:+85113938 None:intergenic
GTTGATAGAGGAATAACAAT+TGG 0.386783 7.4:-85113189 MS.gene91611:CDS
TTTCTTTGAACCAAAAGTTA+AGG 0.387961 7.4:-85113751 MS.gene91611:CDS
TAGAAGAAAATACGTTCAAC+TGG 0.403167 7.4:+85114380 None:intergenic
CAAACAATGTTACCGGCTAT+AGG 0.417276 7.4:+85114260 None:intergenic
AAACAATGTTACCGGCTATA+GGG 0.421018 7.4:+85114261 None:intergenic
AGAAGGCCAAACAATGTTAC+CGG 0.429788 7.4:+85114253 None:intergenic
ACAAAAGTGTGTTGTCCAAA+TGG 0.432381 7.4:-85113677 MS.gene91611:CDS
ATTCAAGCAATAGTAGTTGC+AGG 0.433078 7.4:-85113282 MS.gene91611:CDS
ATGACACTCTCAAAACCATT+TGG 0.448666 7.4:+85113846 None:intergenic
TAGGGTGTGATTGTGAATTT+GGG 0.451765 7.4:+85114351 None:intergenic
GTTGCAGGGTCAACAAGGTT+TGG 0.459696 7.4:-85113267 MS.gene91611:CDS
AATCACTACTCCATCTTCCT+TGG 0.464482 7.4:+85113407 None:intergenic
AGAGTGTCATCATAGATAGT+TGG 0.476458 7.4:-85113833 MS.gene91611:CDS
GTGTGATTGTGAATTTGGGA+GGG 0.498440 7.4:+85114355 None:intergenic
TCTAGATATATGGATATTGT+TGG 0.505579 7.4:-85113312 MS.gene91611:CDS
GAAGTAATCAATGATCCAAA+TGG 0.507008 7.4:-85113861 MS.gene91611:CDS
CAACCATGTTCATCAAAGCT+TGG 0.510827 7.4:-85113962 MS.gene91611:CDS
GTGATTGTGAATTTGGGAGG+GGG 0.512685 7.4:+85114357 None:intergenic
GAAAGTAGAGATTTGAAAGG+TGG 0.519737 7.4:-85113159 MS.gene91611:CDS
CGGCTATAGGGAGAAAGTGT+TGG 0.519995 7.4:+85114273 None:intergenic
AAATGGAACAATATTGAAGA+AGG 0.523989 7.4:-85113660 MS.gene91611:CDS
TGTGATTGTGAATTTGGGAG+GGG 0.526202 7.4:+85114356 None:intergenic
GAAGACTTTACCAAGGAAGA+TGG 0.531066 7.4:-85113417 MS.gene91611:CDS
GAAACTACTTCAGTTGATAG+AGG 0.550153 7.4:-85113201 MS.gene91611:CDS
AGTTTCATTCGATAAAATTG+TGG 0.567981 7.4:-85114150 MS.gene91611:CDS
TATATGGATATTGTTGGTGA+AGG 0.574366 7.4:-85113306 MS.gene91611:CDS
ACAGAAAGTAGAGATTTGAA+AGG 0.576880 7.4:-85113162 MS.gene91611:CDS
ATGATGTGTGCTTATTCCAA+TGG 0.580448 7.4:+85114007 None:intergenic
CTATAGGGAGAAAGTGTTGG+AGG 0.581884 7.4:+85114276 None:intergenic
CAACACTTTCTCCCTATAGC+CGG 0.583671 7.4:-85114272 MS.gene91611:CDS
GGTGTGATTGTGAATTTGGG+AGG 0.587405 7.4:+85114354 None:intergenic
TTCAAGTCTAAGAAAGTGAG+AGG 0.591765 7.4:+85114419 None:intergenic
GAATCTGTTGATTCTAAGTG+TGG 0.592105 7.4:+85114083 None:intergenic
GAGCTCGAAGGCGACTCGAA+CGG 0.608489 7.4:+85113725 None:intergenic
GGACCAAGCTTTGATGAACA+TGG 0.617022 7.4:+85113959 None:intergenic
CGAGATAAAGAAGCTTCTTG+TGG 0.621141 7.4:+85114116 None:intergenic
TAGTAGTTGCAGGGTCAACA+AGG 0.621931 7.4:-85113272 MS.gene91611:CDS
ATCTTCACGTGTGAGCTCGA+AGG 0.625150 7.4:+85113713 None:intergenic
CAGTTCAATCCAAAAGATGA+CGG 0.625889 7.4:-85114209 MS.gene91611:CDS
TTCAAGCAATAGTAGTTGCA+GGG 0.626780 7.4:-85113281 MS.gene91611:CDS
TATAGGGAGAAAGTGTTGGA+GGG 0.645723 7.4:+85114277 None:intergenic
AGAAGAAAATACGTTCAACT+GGG 0.676059 7.4:+85114381 None:intergenic
TGAGCGAGAAGACTTTACCA+AGG 0.738299 7.4:-85113424 MS.gene91611:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAACAAAAATTTTGTTT+TGG - chr7.4:85114040-85114059 MS.gene91611:CDS 10.0%
!! AAAATTAGAAAAATGGATAA+GGG - chr7.4:85114211-85114230 MS.gene91611:CDS 15.0%
!! TTATAGCAAAATTAGAAAAA+TGG - chr7.4:85114204-85114223 MS.gene91611:CDS 15.0%
!! CAAAATTAGAAAAATGGATA+AGG - chr7.4:85114210-85114229 MS.gene91611:CDS 20.0%
!! TTCAAAAGATTTACATATAG+AGG + chr7.4:85113639-85113658 None:intergenic 20.0%
! AAATGGAACAATATTGAAGA+AGG - chr7.4:85113914-85113933 MS.gene91611:CDS 25.0%
! ACAAAAGTTGACAATTTCTT+TGG + chr7.4:85113959-85113978 None:intergenic 25.0%
! AGTTTCATTCGATAAAATTG+TGG - chr7.4:85113424-85113443 MS.gene91611:CDS 25.0%
! TACATTGTTGTCTAGATATA+TGG - chr7.4:85114252-85114271 MS.gene91611:CDS 25.0%
! TCTAGATATATGGATATTGT+TGG - chr7.4:85114262-85114281 MS.gene91611:CDS 25.0%
! TTCTTCAATATTGTTCCATT+TGG + chr7.4:85113915-85113934 None:intergenic 25.0%
! TTTCTTTGAACCAAAAGTTA+AGG - chr7.4:85113823-85113842 MS.gene91611:CDS 25.0%
!! ACAATTTCCGAAATAGTTTT+CGG + chr7.4:85114107-85114126 None:intergenic 25.0%
!!! TATGAGAATGATTTTGGTTT+TGG - chr7.4:85114334-85114353 MS.gene91611:CDS 25.0%
ACAGAAAGTAGAGATTTGAA+AGG - chr7.4:85114412-85114431 MS.gene91611:CDS 30.0%
AGAAGAAAATACGTTCAACT+GGG + chr7.4:85113196-85113215 None:intergenic 30.0%
ATTACTTCCCTACTCAAAAA+GGG + chr7.4:85113701-85113720 None:intergenic 30.0%
CTCAAAAAGGGTTTCAAAAA+AGG + chr7.4:85113689-85113708 None:intergenic 30.0%
GAAGTAATCAATGATCCAAA+TGG - chr7.4:85113713-85113732 MS.gene91611:CDS 30.0%
TAGAAGAAAATACGTTCAAC+TGG + chr7.4:85113197-85113216 None:intergenic 30.0%
TCACTTTCTTAGACTTGAAT+TGG - chr7.4:85113159-85113178 MS.gene91611:CDS 30.0%
! ATAACTCTTTTTCCGAAAAG+TGG - chr7.4:85113539-85113558 MS.gene91611:CDS 30.0%
! CAATTTCCGAAATAGTTTTC+GGG + chr7.4:85114106-85114125 None:intergenic 30.0%
! GGAGTATATGAGAATGATTT+TGG - chr7.4:85114328-85114347 MS.gene91611:CDS 30.0%
! GTTGATAGAGGAATAACAAT+TGG - chr7.4:85114385-85114404 MS.gene91611:CDS 30.0%
! TATATGGATATTGTTGGTGA+AGG - chr7.4:85114268-85114287 MS.gene91611:CDS 30.0%
!! ATCTCAACACATTTTGACAA+AGG - chr7.4:85113770-85113789 MS.gene91611:CDS 30.0%
!! GATAATTGGTTTGCAGAATT+AGG + chr7.4:85113131-85113150 None:intergenic 30.0%
!! TTTTGCTTTTGCTATGCAAA+CGG + chr7.4:85114002-85114021 None:intergenic 30.0%
!!! AAGATTTGAGTTTTGGAACA+AGG + chr7.4:85113265-85113284 None:intergenic 30.0%
!!! AGAATGATTTTGGTTTTGGA+AGG - chr7.4:85114338-85114357 MS.gene91611:CDS 30.0%
!!! AGGTTTTGAAAGAAGAAAGT+AGG + chr7.4:85113245-85113264 None:intergenic 30.0%
!!! ATTTTGTTTTGGTTTTGCAG+TGG - chr7.4:85114051-85114070 MS.gene91611:CDS 30.0%
!!! GGTTTTGAAAGAAGAAAGTA+GGG + chr7.4:85113244-85113263 None:intergenic 30.0%
!!! TTCATACCTTTTTTCGTGAA+GGG - chr7.4:85114482-85114501 MS.gene91611:CDS 30.0%
!!! TTTCATACCTTTTTTCGTGA+AGG - chr7.4:85114481-85114500 MS.gene91611:CDS 30.0%
!!! TTTGAAACCCTTTTTGAGTA+GGG - chr7.4:85113691-85113710 MS.gene91611:CDS 30.0%
!!! TTTTGAAACCCTTTTTGAGT+AGG - chr7.4:85113690-85113709 MS.gene91611:CDS 30.0%
AAGTGAGAGGAAGAGATAAT+TGG + chr7.4:85113145-85113164 None:intergenic 35.0%
ACACATCATGTTGTTCTTGA+TGG - chr7.4:85113581-85113600 MS.gene91611:CDS 35.0%
ATGACACTCTCAAAACCATT+TGG + chr7.4:85113731-85113750 None:intergenic 35.0%
ATGATGTGTGCTTATTCCAA+TGG + chr7.4:85113570-85113589 None:intergenic 35.0%
ATTCAAGCAATAGTAGTTGC+AGG - chr7.4:85114292-85114311 MS.gene91611:CDS 35.0%
CAGTTCAATCCAAAAGATGA+CGG - chr7.4:85113365-85113384 MS.gene91611:CDS 35.0%
CCAATTCCCGAAAACTATTT+CGG - chr7.4:85114097-85114116 MS.gene91611:CDS 35.0%
GAAAGTAGAGATTTGAAAGG+TGG - chr7.4:85114415-85114434 MS.gene91611:CDS 35.0%
GATTACTTCCCTACTCAAAA+AGG + chr7.4:85113702-85113721 None:intergenic 35.0%
TAGGGTGTGATTGTGAATTT+GGG + chr7.4:85113226-85113245 None:intergenic 35.0%
TTCAAGCAATAGTAGTTGCA+GGG - chr7.4:85114293-85114312 MS.gene91611:CDS 35.0%
TTCAAGTCTAAGAAAGTGAG+AGG + chr7.4:85113158-85113177 None:intergenic 35.0%
TTTGGATTGAACTGGATGAT+AGG + chr7.4:85113359-85113378 None:intergenic 35.0%
! AAACAATGTTACCGGCTATA+GGG + chr7.4:85113316-85113335 None:intergenic 35.0%
! ACAAAAGTGTGTTGTCCAAA+TGG - chr7.4:85113897-85113916 MS.gene91611:CDS 35.0%
! AGAGTGTCATCATAGATAGT+TGG - chr7.4:85113741-85113760 MS.gene91611:CDS 35.0%
! ATGGATAAGGGTTTTCTTGA+TGG - chr7.4:85114223-85114242 MS.gene91611:CDS 35.0%
! TTGAAAGGTGGTGTTGAATT+TGG - chr7.4:85114427-85114446 MS.gene91611:CDS 35.0%
! TTGAACTGGATGATAGGTTT+TGG + chr7.4:85113353-85113372 None:intergenic 35.0%
!! AAATAGTTTTCGGGAATTGG+TGG + chr7.4:85114097-85114116 None:intergenic 35.0%
!! CCAATGGAAAAACCACTTTT+CGG + chr7.4:85113554-85113573 None:intergenic 35.0%
!! GAAACTACTTCAGTTGATAG+AGG - chr7.4:85114373-85114392 MS.gene91611:CDS 35.0%
!! GAATCTGTTGATTCTAAGTG+TGG + chr7.4:85113494-85113513 None:intergenic 35.0%
!! GAGAGTGAAGATTTGAGTTT+TGG + chr7.4:85113272-85113291 None:intergenic 35.0%
!! TGAAGTAGTTTCCACTTTTG+CGG + chr7.4:85114365-85114384 None:intergenic 35.0%
!!! ATAGGTTTTGGAGAATCAGA+AGG + chr7.4:85113341-85113360 None:intergenic 35.0%
AATCACTACTCCATCTTCCT+TGG + chr7.4:85114170-85114189 None:intergenic 40.0%
AGAAGGCCAAACAATGTTAC+CGG + chr7.4:85113324-85113343 None:intergenic 40.0%
CAACCATGTTCATCAAAGCT+TGG - chr7.4:85113612-85113631 MS.gene91611:CDS 40.0%
CGAGATAAAGAAGCTTCTTG+TGG + chr7.4:85113461-85113480 None:intergenic 40.0%
CTATAGCCGGTAACATTGTT+TGG - chr7.4:85113315-85113334 MS.gene91611:CDS 40.0%
GAAGACTTTACCAAGGAAGA+TGG - chr7.4:85114157-85114176 MS.gene91611:CDS 40.0%
GCATAACCCTTCACGAAAAA+AGG + chr7.4:85114491-85114510 None:intergenic 40.0%
GTAGGGTGTGATTGTGAATT+TGG + chr7.4:85113227-85113246 None:intergenic 40.0%
GTGTGATTGTGAATTTGGGA+GGG + chr7.4:85113222-85113241 None:intergenic 40.0%
TGTGATTGTGAATTTGGGAG+GGG + chr7.4:85113221-85113240 None:intergenic 40.0%
! AACGAAACTCCGTCATCTTT+TGG + chr7.4:85113377-85113396 None:intergenic 40.0%
! CAAACAATGTTACCGGCTAT+AGG + chr7.4:85113317-85113336 None:intergenic 40.0%
! CCGAAAAGTGGTTTTTCCAT+TGG - chr7.4:85113551-85113570 MS.gene91611:CDS 40.0%
! CGAACGGAATCCTTAACTTT+TGG + chr7.4:85113836-85113855 None:intergenic 40.0%
! CGTCATCTTTTGGATTGAAC+TGG + chr7.4:85113367-85113386 None:intergenic 40.0%
!! CCGAAATAGTTTTCGGGAAT+TGG + chr7.4:85114100-85114119 None:intergenic 40.0%
!! GAAGTAGTTTCCACTTTTGC+GGG + chr7.4:85114364-85114383 None:intergenic 40.0%
!! TATAGGGAGAAAGTGTTGGA+GGG + chr7.4:85113300-85113319 None:intergenic 40.0%
CAACACTTTCTCCCTATAGC+CGG - chr7.4:85113302-85113321 MS.gene91611:CDS 45.0%
GGTGTGATTGTGAATTTGGG+AGG + chr7.4:85113223-85113242 None:intergenic 45.0%
GTGATTGTGAATTTGGGAGG+GGG + chr7.4:85113220-85113239 None:intergenic 45.0%
TAGTAGTTGCAGGGTCAACA+AGG - chr7.4:85114302-85114321 MS.gene91611:CDS 45.0%
TGAGCGAGAAGACTTTACCA+AGG - chr7.4:85114150-85114169 MS.gene91611:CDS 45.0%
! GGACCAAGCTTTGATGAACA+TGG + chr7.4:85113618-85113637 None:intergenic 45.0%
!! CTATAGGGAGAAAGTGTTGG+AGG + chr7.4:85113301-85113320 None:intergenic 45.0%
ATCTTCACGTGTGAGCTCGA+AGG + chr7.4:85113864-85113883 None:intergenic 50.0%
CGAGCTCACACGTGAAGATT+TGG - chr7.4:85113865-85113884 MS.gene91611:CDS 50.0%
GTTGCAGGGTCAACAAGGTT+TGG - chr7.4:85114307-85114326 MS.gene91611:CDS 50.0%
! CGGCTATAGGGAGAAAGTGT+TGG + chr7.4:85113304-85113323 None:intergenic 50.0%
!! TTTTGGAAGGCCCGCAAAAG+TGG - chr7.4:85114351-85114370 MS.gene91611:CDS 50.0%
GAGCTCGAAGGCGACTCGAA+CGG + chr7.4:85113852-85113871 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 85113077 85114519 85113077 ID=MS.gene91611
chr7.4 mRNA 85113077 85114519 85113077 ID=MS.gene91611.t1;Parent=MS.gene91611
chr7.4 exon 85113077 85114519 85113077 ID=MS.gene91611.t1.exon1;Parent=MS.gene91611.t1
chr7.4 CDS 85113077 85114519 85113077 ID=cds.MS.gene91611.t1;Parent=MS.gene91611.t1
Gene Sequence

>MS.gene91611

ATGGCTGCTGCAAACAATCACATCAAAATTCATGAAGAATGTAAAGTTTCTCCTAATTCTGCAAACCAATTATCTCTTCCTCTCACTTTCTTAGACTTGAATTGGCTAAGATTTCACCCAGTTGAACGTATTTTCTTCTACCCCCTCCCAAATTCACAATCACACCCTACTTTCTTCTTTCAAAACCTTGTTCCAAAACTCAAATCTTCACTCTCTTTAACCCTCCAACACTTTCTCCCTATAGCCGGTAACATTGTTTGGCCTTCTGATTCTCCAAAACCTATCATCCAGTTCAATCCAAAAGATGACGGAGTTTCGTTGCTCTTAGCGCAATATGATGACGATGTAGTTTCATTCGATAAAATTGTGGAGCAGAATTCACCACAAGAAGCTTCTTTATCTCGTTCTTTTGTACCACACTTAGAATCAACAGATTCTTTTGCTTCTATCATTTCAATTCAGATAACTCTTTTTCCGAAAAGTGGTTTTTCCATTGGAATAAGCACACATCATGTTGTTCTTGATGGAAAATCTTCAACCATGTTCATCAAAGCTTGGTCCTCTATATGTAAATCTTTTGAAGATGAAACACAATCATCAAATTTAGAGCCTTTTTTGAAACCCTTTTTGAGTAGGGAAGTAATCAATGATCCAAATGGTTTTGAGAGTGTCATCATAGATAGTTGGAACAGAATCTCAACACATTTTGACAAAGGCAACGTGAGAAGCTTAAAGATTATGTCAAATTTCTTTGAACCAAAAGTTAAGGATTCCGTTCGAGTCGCCTTCGAGCTCACACGTGAAGATTTGGAGAAATTAAACAAAAGTGTGTTGTCCAAATGGAACAATATTGAAGAAGGATCACAAGAACAAGAACAACCAAAGAAATTGTCAACTTTTGTTCTTACATGTGCTTATGTTTCCGTTTGCATAGCAAAAGCAAAACAACAATCTGAAAGCAATAAAAAACAAAAATTTTGTTTTGGTTTTGCAGTGGATATCAGATCAAGATTAGATCCACCAATTCCCGAAAACTATTTCGGAAATTGTGTAACTAGTCATATAATTGATACTGAGCGAGAAGACTTTACCAAGGAAGATGGAGTAGTGATTGTTGCAAAGAAAATTTATAGCAAAATTAGAAAAATGGATAAGGGTTTTCTTGATGGTGTAGATACATTGTTGTCTAGATATATGGATATTGTTGGTGAAGGAATTCAAGCAATAGTAGTTGCAGGGTCAACAAGGTTTGGAGTATATGAGAATGATTTTGGTTTTGGAAGGCCCGCAAAAGTGGAAACTACTTCAGTTGATAGAGGAATAACAATTGGATTAACAGAAAGTAGAGATTTGAAAGGTGGTGTTGAATTTGGACTTGTTCTTGATAAGAATGTGATGAATTTTTTTCATACCTTTTTTCGTGAAGGGTTATGCTTTGATTGA
Protein sequence

>MS.gene91611.t1

MAAANNHIKIHEECKVSPNSANQLSLPLTFLDLNWLRFHPVERIFFYPLPNSQSHPTFFFQNLVPKLKSSLSLTLQHFLPIAGNIVWPSDSPKPIIQFNPKDDGVSLLLAQYDDDVVSFDKIVEQNSPQEASLSRSFVPHLESTDSFASIISIQITLFPKSGFSIGISTHHVVLDGKSSTMFIKAWSSICKSFEDETQSSNLEPFLKPFLSREVINDPNGFESVIIDSWNRISTHFDKGNVRSLKIMSNFFEPKVKDSVRVAFELTREDLEKLNKSVLSKWNNIEEGSQEQEQPKKLSTFVLTCAYVSVCIAKAKQQSESNKKQKFCFGFAVDIRSRLDPPIPENYFGNCVTSHIIDTEREDFTKEDGVVIVAKKIYSKIRKMDKGFLDGVDTLLSRYMDIVGEGIQAIVVAGSTRFGVYENDFGFGRPAKVETTSVDRGITIGLTESRDLKGGVEFGLVLDKNVMNFFHTFFREGLCFD