AlfalfaGEDB | MS.gene91609

M. sativa cultivar XinJiangDaYe / MS.gene91609

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016740 GO:0016746 GO:0016747
PFAM: PF02458.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91609.t1	RHN44407.1	86.9	480	63	0	1	480	1	480	3.60E-238	833.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91609.t1	Q940Z5	36.5	479	274	14	8	477	10	467	6.4e-74	279.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91609.t1	A0A396H0H8	86.9	480	63	0	1	480	1	480	2.6e-238	833.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049356	MS.gene91609	0.837113	6.29E-57	-1.69E-46
MS.gene050670	MS.gene91609	0.816341	5.81E-52	-1.69E-46
MS.gene052414	MS.gene91609	0.80274	4.86E-49	-1.69E-46
MS.gene055486	MS.gene91609	0.814339	1.62E-51	-1.69E-46
MS.gene055909	MS.gene91609	0.806874	6.65E-50	-1.69E-46
MS.gene055911	MS.gene91609	0.807151	5.82E-50	-1.69E-46
MS.gene056422	MS.gene91609	0.802625	5.13E-49	-1.69E-46
MS.gene056511	MS.gene91609	0.852618	4.10E-61	-1.69E-46
MS.gene057734	MS.gene91609	0.819481	1.14E-52	-1.69E-46
MS.gene058075	MS.gene91609	0.822259	2.61E-53	-1.69E-46
MS.gene058928	MS.gene91609	0.802516	5.41E-49	-1.69E-46
MS.gene059366	MS.gene91609	0.818556	1.84E-52	-1.69E-46
MS.gene059368	MS.gene91609	0.817529	3.15E-52	-1.69E-46
MS.gene059420	MS.gene91609	0.803696	3.08E-49	-1.69E-46
MS.gene059807	MS.gene91609	0.825931	3.58E-54	-1.69E-46
MS.gene060837	MS.gene91609	0.815773	7.79E-52	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene91609	MS.gene56799	PPI
MS.gene91609	MS.gene32261	PPI
MS.gene91609	MS.gene063868	PPI
MS.gene91609	MS.gene44640	PPI
MS.gene91609	MS.gene032349	PPI
MS.gene91609	MS.gene069767	PPI
MS.gene99222	MS.gene91609	PPI
MS.gene91609	MS.gene021904	PPI
MS.gene91609	MS.gene000763	PPI
MS.gene91609	MS.gene91613	PPI
MS.gene91609	MS.gene011716	PPI
MS.gene91609	MS.gene44633	PPI
MS.gene91609	MS.gene91595	PPI
MS.gene91609	MS.gene90491	PPI
MS.gene066660	MS.gene91609	PPI
MS.gene91609	MS.gene043352	PPI
MS.gene91609	MS.gene50384	PPI
MS.gene91609	MS.gene38977	PPI
MS.gene91609	MS.gene021909	PPI
MS.gene91609	MS.gene066658	PPI
MS.gene91609	MS.gene91612	PPI
MS.gene91609	MS.gene021917	PPI
MS.gene069423	MS.gene91609	PPI
MS.gene91609	MS.gene028998	PPI
MS.gene91609	MS.gene064101	PPI
MS.gene35744	MS.gene91609	PPI
MS.gene028999	MS.gene91609	PPI
MS.gene91609	MS.gene99221	PPI
MS.gene91609	MS.gene31545	PPI
MS.gene91609	MS.gene021910	PPI
MS.gene50379	MS.gene91609	PPI
MS.gene91609	MS.gene063865	PPI
MS.gene91609	MS.gene50356	PPI
MS.gene91609	MS.gene56797	PPI
MS.gene91609	MS.gene011837	PPI
MS.gene91609	MS.gene44630	PPI
MS.gene91609	MS.gene069422	PPI
MS.gene91609	MS.gene066634	PPI
MS.gene073299	MS.gene91609	PPI
MS.gene91597	MS.gene91609	PPI
MS.gene021916	MS.gene91609	PPI
MS.gene91609	MS.gene78818	PPI
MS.gene32261	MS.gene91609	PPI
MS.gene91609	MS.gene013787	PPI
MS.gene91609	MS.gene28251	PPI
MS.gene31546	MS.gene91609	PPI
MS.gene35741	MS.gene91609	PPI
MS.gene91609	MS.gene99219	PPI
MS.gene99219	MS.gene91609	PPI
MS.gene91609	MS.gene016532	PPI
MS.gene91609	MS.gene44647	PPI
MS.gene91609	MS.gene066701	PPI
MS.gene91609	MS.gene021915	PPI
MS.gene91609	MS.gene50355	PPI
MS.gene56795	MS.gene91609	PPI
MS.gene91609	MS.gene011799	PPI
MS.gene91609	MS.gene44643	PPI
MS.gene91609	MS.gene002544	PPI
MS.gene028996	MS.gene91609	PPI
MS.gene91609	MS.gene50420	PPI
MS.gene91609	MS.gene46265	PPI
MS.gene91609	MS.gene070270	PPI
MS.gene91615	MS.gene91609	PPI
MS.gene91609	MS.gene50382	PPI
MS.gene48582	MS.gene91609	PPI
MS.gene91609	MS.gene90496	PPI
MS.gene91609	MS.gene87536	PPI
MS.gene91609	MS.gene99218	PPI
MS.gene91598	MS.gene91609	PPI
MS.gene91609	MS.gene44463	PPI
MS.gene99223	MS.gene91609	PPI
MS.gene91609	MS.gene48584	PPI
MS.gene91609	MS.gene004218	PPI
MS.gene91609	MS.gene50379	PPI
MS.gene066634	MS.gene91609	PPI
MS.gene043346	MS.gene91609	PPI
MS.gene91609	MS.gene016477	PPI
MS.gene91609	MS.gene021913	PPI
MS.gene91609	MS.gene069423	PPI
MS.gene91609	MS.gene91611	PPI
MS.gene91609	MS.gene91599	PPI
MS.gene91609	MS.gene02573	PPI
MS.gene91609	MS.gene011798	PPI
MS.gene91609	MS.gene35740	PPI
MS.gene91609	MS.gene028994	PPI
MS.gene49853	MS.gene91609	PPI
MS.gene007831	MS.gene91609	PPI
MS.gene91609	MS.gene87577	PPI
MS.gene91609	MS.gene021905	PPI
MS.gene44644	MS.gene91609	PPI
MS.gene35745	MS.gene91609	PPI
MS.gene011796	MS.gene91609	PPI
MS.gene87537	MS.gene91609	PPI
MS.gene91609	MS.gene000764	PPI
MS.gene60208	MS.gene91609	PPI
MS.gene91609	MS.gene91614	PPI
MS.gene91609	MS.gene90492	PPI
MS.gene91609	MS.gene043350	PPI
MS.gene013787	MS.gene91609	PPI
MS.gene066656	MS.gene91609	PPI
MS.gene91609	MS.gene56798	PPI
MS.gene31542	MS.gene91609	PPI
MS.gene87532	MS.gene91609	PPI
MS.gene91609	MS.gene87191	PPI
MS.gene91609	MS.gene91598	PPI
MS.gene91609	MS.gene56796	PPI
MS.gene91609	MS.gene021906	PPI
MS.gene91609	MS.gene44642	PPI
MS.gene91609	MS.gene96337	PPI
MS.gene91609	MS.gene87496	PPI
MS.gene91609	MS.gene021916	PPI
MS.gene91609	MS.gene070019	PPI
MS.gene91609	MS.gene043348	PPI
MS.gene91609	MS.gene48585	PPI
MS.gene03202	MS.gene91609	PPI
MS.gene91609	MS.gene011801	PPI
MS.gene91609	MS.gene90493	PPI
MS.gene91609	MS.gene87532	PPI
MS.gene91609	MS.gene91596	PPI
MS.gene91609	MS.gene021914	PPI
MS.gene91609	MS.gene48581	PPI
MS.gene91609	MS.gene31540	PPI
MS.gene012360	MS.gene91609	PPI
MS.gene91609	MS.gene066657	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91609.t1	MTR_7g013850	77.640	483	105	2	1	480	1	483	0.0	767
MS.gene91609.t1	MTR_7g014200	59.627	483	174	12	1	477	1	468	0.0	550
MS.gene91609.t1	MTR_7g014160	57.380	481	190	10	5	480	6	476	0.0	540
MS.gene91609.t1	MTR_7g014610	58.557	485	185	12	1	478	1	476	0.0	517
MS.gene91609.t1	MTR_7g014620	51.828	465	186	9	19	480	6	435	8.90e-150	434
MS.gene91609.t1	MTR_6g015320	39.501	481	260	10	1	477	1	454	5.31e-106	323
MS.gene91609.t1	MTR_6g015315	39.368	475	263	10	4	477	2	452	1.11e-104	320
MS.gene91609.t1	MTR_6g015850	40.900	489	245	16	1	477	1	457	1.29e-103	317
MS.gene91609.t1	MTR_7g014630	48.883	358	164	7	6	350	7	358	1.64e-103	313
MS.gene91609.t1	MTR_6g015815	41.895	475	234	15	11	477	20	460	6.62e-102	313
MS.gene91609.t1	MTR_6g015830	40.748	481	244	14	9	477	9	460	2.31e-101	311
MS.gene91609.t1	MTR_7g014330	38.797	482	254	15	9	477	3	456	5.26e-99	305
MS.gene91609.t1	MTR_7g014230	38.622	479	257	12	9	476	5	457	2.26e-98	304
MS.gene91609.t1	MTR_7g014250	37.866	478	264	12	9	477	3	456	2.48e-97	301
MS.gene91609.t1	MTR_3g028210	35.052	485	272	15	8	477	8	464	3.05e-77	249
MS.gene91609.t1	MTR_7g014180	67.039	179	52	5	1	178	1	173	9.23e-71	223
MS.gene91609.t1	MTR_7g014670	56.316	190	81	2	289	477	71	259	7.95e-68	218
MS.gene91609.t1	MTR_2g089765	31.352	488	288	14	11	477	21	482	5.19e-67	223
MS.gene91609.t1	MTR_7g014360	33.171	410	222	12	76	477	21	386	6.31e-65	214
MS.gene91609.t1	MTR_3g087640	31.352	488	291	16	9	477	10	472	3.46e-60	205
MS.gene91609.t1	MTR_2g089650	29.897	485	295	12	8	477	9	463	4.47e-60	204
MS.gene91609.t1	MTR_2g089670	31.410	468	272	14	26	477	43	477	1.58e-59	203
MS.gene91609.t1	MTR_7g015860	32.305	486	266	16	8	468	5	452	1.14e-58	200
MS.gene91609.t1	MTR_2g089755	30.769	468	277	14	28	477	37	475	1.38e-56	195
MS.gene91609.t1	MTR_2g089735	31.473	448	271	12	41	477	39	461	6.69e-56	193
MS.gene91609.t1	MTR_6g015310	32.593	405	200	12	51	455	2	333	3.13e-55	187
MS.gene91609.t1	MTR_8g012750	30.704	469	284	16	26	475	24	470	1.21e-54	190
MS.gene91609.t1	MTR_7g014310	27.246	345	183	9	117	460	29	306	2.11e-34	131
MS.gene91609.t1	MTR_3g435300	33.333	234	131	7	7	229	3	222	3.02e-30	119
MS.gene91609.t1	MTR_2g089665	25.110	454	205	15	11	454	7	335	3.97e-26	109
MS.gene91609.t1	MTR_3g070170	24.892	462	272	21	8	439	12	428	1.98e-21	97.1
MS.gene91609.t1	MTR_4g007540	24.374	439	260	17	8	429	3	386	1.09e-20	94.7
MS.gene91609.t1	MTR_6g045553	24.248	499	291	20	7	467	13	462	5.68e-20	92.8
MS.gene91609.t1	MTR_8g075610	24.365	394	241	16	44	429	30	374	2.32e-19	90.5
MS.gene91609.t1	MTR_3g101900	24.229	454	280	17	28	466	30	434	5.11e-19	89.7
MS.gene91609.t1	MTR_7g086000	23.896	385	235	10	67	429	60	408	1.73e-18	88.2
MS.gene91609.t1	MTR_3g026950	24.138	435	269	17	64	474	51	448	4.10e-18	87.0
MS.gene91609.t1	MTR_3g049150	24.145	497	299	21	8	477	2	447	4.13e-18	87.0
MS.gene91609.t1	MTR_2g064495	22.757	457	289	19	31	475	27	431	7.87e-17	82.8
MS.gene91609.t1	MTR_2g105330	22.976	457	288	19	31	475	27	431	2.11e-16	81.6
MS.gene91609.t1	MTR_5g081960	23.859	482	321	18	13	467	11	473	3.55e-16	81.3
MS.gene91609.t1	MTR_2g007880	24.622	463	235	22	51	470	49	440	5.86e-16	80.5
MS.gene91609.t1	MTR_4g094842	23.833	407	225	17	62	439	57	407	2.39e-15	78.6
MS.gene91609.t1	MTR_7g014210	33.645	107	69	2	335	439	24	130	4.52e-15	73.2
MS.gene91609.t1	MTR_4g097090	24.471	425	253	19	26	428	28	406	4.86e-15	77.4
MS.gene91609.t1	MTR_2g015430	22.876	459	282	15	8	456	2	398	6.17e-15	77.4
MS.gene91609.t1	MTR_3g026310	22.517	453	268	17	17	441	14	411	8.14e-15	77.0
MS.gene91609.t1	MTR_7g085930	24.928	349	227	12	4	349	2	318	3.25e-14	75.1
MS.gene91609.t1	MTR_3g102020	23.841	453	265	21	13	439	11	409	3.86e-14	74.7
MS.gene91609.t1	MTR_4g127650	21.806	454	294	15	41	475	43	454	4.91e-14	74.3
MS.gene91609.t1	MTR_7g014205	29.839	124	75	2	317	439	2	114	9.42e-14	68.2
MS.gene91609.t1	MTR_8g096530	25.287	435	255	17	7	429	6	382	1.16e-13	73.2
MS.gene91609.t1	MTR_8g467580	24.249	433	246	18	17	429	15	385	5.52e-12	68.2
MS.gene91609.t1	MTR_7g014340	53.521	71	26	2	9	75	3	70	6.29e-12	62.4
MS.gene91609.t1	MTR_0190s0020	23.488	430	260	16	25	439	26	401	1.23e-11	67.0
MS.gene91609.t1	MTR_4g015180	21.622	481	338	14	10	466	8	473	3.58e-11	65.5
MS.gene91609.t1	MTR_3g098980	25.260	289	170	12	67	351	66	312	4.96e-11	65.1
MS.gene91609.t1	MTR_7g014220	58.491	53	19	1	25	77	15	64	5.85e-11	58.9
MS.gene91609.t1	MTR_8g467590	24.762	420	229	20	44	442	41	394	7.39e-11	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91609.t1	AT3G29635	39.083	458	247	13	24	477	25	454	6.29e-90	282
MS.gene91609.t1	AT5G39050	37.996	479	267	14	8	477	10	467	6.38e-89	280
MS.gene91609.t1	AT3G29590	36.217	497	248	10	1	477	1	448	8.34e-89	279
MS.gene91609.t1	AT5G39080	37.630	481	270	14	1	474	1	458	3.19e-87	275
MS.gene91609.t1	AT5G39090	36.250	480	270	13	1	477	1	447	2.66e-83	265
MS.gene91609.t1	AT3G29670	36.743	479	262	14	8	477	3	449	8.51e-83	263
MS.gene91609.t1	AT5G61160	34.038	473	280	13	8	475	3	448	3.86e-78	251
MS.gene91609.t1	AT3G29680	35.983	478	262	14	4	473	2	443	5.47e-75	243
MS.gene91609.t1	AT1G03940	29.541	501	292	14	1	477	1	464	9.93e-62	209
MS.gene91609.t1	AT1G03495	30.376	372	216	13	1	359	1	342	6.05e-40	147
MS.gene91609.t1	AT5G23940	24.569	464	262	21	8	439	3	410	7.03e-23	101
MS.gene91609.t1	AT3G29720	35.616	219	100	10	99	303	10	201	5.46e-20	89.0
MS.gene91609.t1	AT5G67150	24.766	428	273	13	16	434	13	400	7.70e-18	86.3
MS.gene91609.t1	AT3G50280	23.776	429	265	18	19	434	18	397	2.11e-17	84.7
MS.gene91609.t1	AT3G29690	31.126	151	98	2	328	477	33	178	3.73e-16	77.0
MS.gene91609.t1	AT2G39980	23.307	502	315	18	1	467	1	467	2.79e-15	78.6
MS.gene91609.t1	AT3G47170	24.620	329	199	12	47	365	57	346	2.20e-12	69.3
MS.gene91609.t1	AT2G25150	22.642	424	256	19	67	466	67	442	2.60e-12	69.3
MS.gene91609.t1	AT4G31910	20.798	351	245	8	44	389	46	368	2.72e-11	65.9

Find 67 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TACATTGTTGTCTAAATATT+TGG	0.130040	7.4:-85085710	MS.gene91609:CDS
GATTACTTCCCTACTCAAAA+AGG	0.196644	7.4:+85086263	None:intergenic
TTGAAAGGTGGTGTTGAATT+TGG	0.214452	7.4:-85085535	MS.gene91609:CDS
GAGAGTGAAGATTTGAGTTT+TGG	0.216722	7.4:+85086693	None:intergenic
GATAATTGGTTTGCAGAATT+AGG	0.230849	7.4:+85086834	None:intergenic
TTCAAAAGATTTACATATAA+AGG	0.255816	7.4:+85086326	None:intergenic
ACAAAAGTTGACAATTTCTT+TGG	0.263553	7.4:+85086006	None:intergenic
AACGAAACTCCGTCATCTTT+TGG	0.264453	7.4:+85086588	None:intergenic
GGAGTATATGAGATTGATTT+TGG	0.273264	7.4:-85085634	MS.gene91609:CDS
AAGTGAGAGGAAGAGATAAT+TGG	0.284573	7.4:+85086820	None:intergenic
CGAACGGAATCCTTAACTTT+TGG	0.284718	7.4:+85086129	None:intergenic
TTCTTCAATATTGTTCCATT+TGG	0.285958	7.4:+85086050	None:intergenic
TCTTCCTTGGTAAAGTCTTC+TGG	0.306022	7.4:+85085808	None:intergenic
CGAGCTCACACGTGAAGATT+TGG	0.319044	7.4:-85086097	MS.gene91609:CDS
TGAAAGGTGGTGTTGAATTT+GGG	0.328871	7.4:-85085534	MS.gene91609:CDS
CTATAGCCGGTAACATTGTT+TGG	0.330391	7.4:-85086647	MS.gene91609:CDS
CCAATTCCCGAAAACTATTT+CGG	0.342065	7.4:-85085865	MS.gene91609:CDS
TCACTTTCTTAGACTTGAAT+TGG	0.344769	7.4:-85086803	MS.gene91609:CDS
GTTGATAGAGGAATAACAAT+TGG	0.386783	7.4:-85085577	MS.gene91609:CDS
TTTCCTTGAACCAAAAGTTA+AGG	0.387961	7.4:-85086139	MS.gene91609:CDS
TCTAAATATTTGGCTATGAT+TGG	0.398839	7.4:-85085700	MS.gene91609:CDS
TAGAAGAAAATACGTTCAAC+TGG	0.403167	7.4:+85086768	None:intergenic
ACACATCATGCTGTTCTTGA+TGG	0.407163	7.4:-85086381	MS.gene91609:CDS
ATTCAAGGAATAGGAGTTGC+AGG	0.408252	7.4:-85085670	MS.gene91609:CDS
CAAACAATGTTACCGGCTAT+AGG	0.417276	7.4:+85086648	None:intergenic
AAACAATGTTACCGGCTATA+GGG	0.421018	7.4:+85086649	None:intergenic
TTTGGATTGAACTGGATGAT+TGG	0.424802	7.4:+85086606	None:intergenic
GGTGAAGGAATTCAAGGAAT+AGG	0.425888	7.4:-85085679	MS.gene91609:CDS
AGAAGGCCAAACAATGTTAC+CGG	0.429788	7.4:+85086641	None:intergenic
ACAAAAGTGTGTTGTCCAAA+TGG	0.432381	7.4:-85086065	MS.gene91609:CDS
GTTGCAGGGTCAACAAGGTT+TGG	0.454958	7.4:-85085655	MS.gene91609:CDS
AGGACACTCTCAAAACCATT+TGG	0.455320	7.4:+85086234	None:intergenic
AATCACTACTCCATCTTCCT+TGG	0.466306	7.4:+85085795	None:intergenic
AGAGTGTCCTCATAGATAGT+TGG	0.473788	7.4:-85086221	MS.gene91609:CDS
TGTTCTTGATAAGCATGTGA+TGG	0.498965	7.4:-85085509	MS.gene91609:CDS
ATGATTGGTGAAGGAATTCA+AGG	0.503765	7.4:-85085685	MS.gene91609:CDS
GAAGTAATCAATGATCCAAA+TGG	0.507008	7.4:-85086249	MS.gene91609:CDS
GTTCTTGATAAGCATGTGAT+GGG	0.507272	7.4:-85085508	MS.gene91609:CDS
CAACCATGTTCATCAAAGCT+TGG	0.510827	7.4:-85086350	MS.gene91609:CDS
GAAAGTAGAGATTTGAAAGG+TGG	0.519737	7.4:-85085547	MS.gene91609:CDS
CGGCTATAGGGAGAAAGTGT+TGG	0.519995	7.4:+85086661	None:intergenic
AAATGGAACAATATTGAAGA+AGG	0.523989	7.4:-85086048	MS.gene91609:CDS
TTATGGAAGGCCTGCAAAAG+TGG	0.524298	7.4:-85085611	MS.gene91609:CDS
TTCTGTTCCAACTATCTATG+AGG	0.538513	7.4:+85086214	None:intergenic
GAAGACTTTACCAAGGAAGA+TGG	0.540696	7.4:-85085805	MS.gene91609:CDS
GTGTGATTGTGAATTTGAGA+GGG	0.543052	7.4:+85086743	None:intergenic
GAGACAACTTCAGTTGATAG+AGG	0.566864	7.4:-85085589	MS.gene91609:CDS
AGTTTCCTTTGATAAAATTG+TGG	0.567981	7.4:-85086538	MS.gene91609:CDS
ACAGAAAGTAGAGATTTGAA+AGG	0.576880	7.4:-85085550	MS.gene91609:CDS
TATTTGGCTATGATTGGTGA+AGG	0.577286	7.4:-85085694	MS.gene91609:CDS
CTATAGGGAGAAAGTGTTGG+AGG	0.581884	7.4:+85086664	None:intergenic
CAACACTTTCTCCCTATAGC+CGG	0.583671	7.4:-85086660	MS.gene91609:CDS
TTCAAGTCTAAGAAAGTGAG+AGG	0.591765	7.4:+85086807	None:intergenic
GAATCTATTGATTCTAAGTG+AGG	0.594969	7.4:+85086471	None:intergenic
GGTGTGATTGTGAATTTGAG+AGG	0.596378	7.4:+85086742	None:intergenic
GGACCAAGCTTTGATGAACA+TGG	0.599628	7.4:+85086347	None:intergenic
GAGCTCGAAGGCGACTCGAA+CGG	0.608489	7.4:+85086113	None:intergenic
TAGGAGTTGCAGGGTCAACA+AGG	0.619039	7.4:-85085660	MS.gene91609:CDS
ATCTTCACGTGTGAGCTCGA+AGG	0.625150	7.4:+85086101	None:intergenic
CAGTTCAATCCAAAAGATGA+CGG	0.625889	7.4:-85086597	MS.gene91609:CDS
TATAGGGAGAAAGTGTTGGA+GGG	0.645723	7.4:+85086665	None:intergenic
ATGATGTGTGCTTATTCCAA+TGG	0.647501	7.4:+85086395	None:intergenic
GTGATTGTGAATTTGAGAGG+GGG	0.652255	7.4:+85086745	None:intergenic
TGTGATTGTGAATTTGAGAG+GGG	0.656243	7.4:+85086744	None:intergenic
TTCAAGGAATAGGAGTTGCA+GGG	0.658447	7.4:-85085669	MS.gene91609:CDS
AGAAGAAAATACGTTCAACT+GGG	0.676059	7.4:+85086769	None:intergenic
TGAGCCAGAAGACTTTACCA+AGG	0.738299	7.4:-85085812	MS.gene91609:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATTAGAAAAATGGATAA+GGG	-	chr7.4:85086599-85086618	MS.gene91609:CDS	15.0%
!!	TTATAGCAAAATTAGAAAAA+TGG	-	chr7.4:85086592-85086611	MS.gene91609:CDS	15.0%
!!	TTCAAAAGATTTACATATAA+AGG	+	chr7.4:85086027-85086046	None:intergenic	15.0%
!!!	CAAAAACAAAAATTTTGTTT+TGG	-	chr7.4:85086428-85086447	MS.gene91609:CDS	15.0%
!!	CAAAATTAGAAAAATGGATA+AGG	-	chr7.4:85086598-85086617	MS.gene91609:CDS	20.0%
!!	TACATTGTTGTCTAAATATT+TGG	-	chr7.4:85086640-85086659	MS.gene91609:CDS	20.0%
!	AAATGGAACAATATTGAAGA+AGG	-	chr7.4:85086302-85086321	MS.gene91609:CDS	25.0%
!	ACAAAAGTTGACAATTTCTT+TGG	+	chr7.4:85086347-85086366	None:intergenic	25.0%
!	AGTTTCCTTTGATAAAATTG+TGG	-	chr7.4:85085812-85085831	MS.gene91609:CDS	25.0%
!	TCTAAATATTTGGCTATGAT+TGG	-	chr7.4:85086650-85086669	MS.gene91609:CDS	25.0%
!	TTCTTCAATATTGTTCCATT+TGG	+	chr7.4:85086303-85086322	None:intergenic	25.0%
!!	ACAATTTCCGAAATAGTTTT+CGG	+	chr7.4:85086495-85086514	None:intergenic	25.0%
!!!	TATGAGATTGATTTTGGTTA+TGG	-	chr7.4:85086722-85086741	MS.gene91609:CDS	25.0%
!!!	TTCATACCATTTTTCATGAA+GGG	-	chr7.4:85086870-85086889	MS.gene91609:CDS	25.0%
!!!	TTTCATACCATTTTTCATGA+AGG	-	chr7.4:85086869-85086888	MS.gene91609:CDS	25.0%
	ACAGAAAGTAGAGATTTGAA+AGG	-	chr7.4:85086800-85086819	MS.gene91609:CDS	30.0%
	AGAAGAAAATACGTTCAACT+GGG	+	chr7.4:85085584-85085603	None:intergenic	30.0%
	ATTACTTCCCTACTCAAAAA+GGG	+	chr7.4:85086089-85086108	None:intergenic	30.0%
	CTCAAAAAGGGTTTCAAAAA+AGG	+	chr7.4:85086077-85086096	None:intergenic	30.0%
	GAAGTAATCAATGATCCAAA+TGG	-	chr7.4:85086101-85086120	MS.gene91609:CDS	30.0%
	TAGAAGAAAATACGTTCAAC+TGG	+	chr7.4:85085585-85085604	None:intergenic	30.0%
	TCACTTTCTTAGACTTGAAT+TGG	-	chr7.4:85085547-85085566	MS.gene91609:CDS	30.0%
	TTTCCTTGAACCAAAAGTTA+AGG	-	chr7.4:85086211-85086230	MS.gene91609:CDS	30.0%
!	ATAACTCTTTTTCCGAAAAG+TGG	-	chr7.4:85085927-85085946	MS.gene91609:CDS	30.0%
!	CAATTTCCGAAATAGTTTTC+GGG	+	chr7.4:85086494-85086513	None:intergenic	30.0%
!	GTTGATAGAGGAATAACAAT+TGG	-	chr7.4:85086773-85086792	MS.gene91609:CDS	30.0%
!!	ATCTCAACACATTTTGACAA+AGG	-	chr7.4:85086158-85086177	MS.gene91609:CDS	30.0%
!!	GAATCTATTGATTCTAAGTG+AGG	+	chr7.4:85085882-85085901	None:intergenic	30.0%
!!	GATAATTGGTTTGCAGAATT+AGG	+	chr7.4:85085519-85085538	None:intergenic	30.0%
!!	GGAGTATATGAGATTGATTT+TGG	-	chr7.4:85086716-85086735	MS.gene91609:CDS	30.0%
!!	TTTTGCTTTTGCTATGCAAA+CGG	+	chr7.4:85086390-85086409	None:intergenic	30.0%
!!!	AAGATTTGAGTTTTGGAACA+AGG	+	chr7.4:85085653-85085672	None:intergenic	30.0%
!!!	AATCCTTAACTTTTGGTTCA+AGG	+	chr7.4:85086217-85086236	None:intergenic	30.0%
!!!	AGATTGATTTTGGTTATGGA+AGG	-	chr7.4:85086726-85086745	MS.gene91609:CDS	30.0%
!!!	AGGTTTTGAAAGAAGAAAGT+AGG	+	chr7.4:85085633-85085652	None:intergenic	30.0%
!!!	ATTGGTTTTGAAGAATCAGA+AGG	+	chr7.4:85085729-85085748	None:intergenic	30.0%
!!!	ATTTTGTTTTGGTTTTGCAG+TGG	-	chr7.4:85086439-85086458	MS.gene91609:CDS	30.0%
!!!	GGTTTTGAAAGAAGAAAGTA+GGG	+	chr7.4:85085632-85085651	None:intergenic	30.0%
!!!	TTTGAAACCCTTTTTGAGTA+GGG	-	chr7.4:85086079-85086098	MS.gene91609:CDS	30.0%
!!!	TTTTGAAACCCTTTTTGAGT+AGG	-	chr7.4:85086078-85086097	MS.gene91609:CDS	30.0%
	AAGTGAGAGGAAGAGATAAT+TGG	+	chr7.4:85085533-85085552	None:intergenic	35.0%
	ATGATGTGTGCTTATTCCAA+TGG	+	chr7.4:85085958-85085977	None:intergenic	35.0%
	ATGATTGGTGAAGGAATTCA+AGG	-	chr7.4:85086665-85086684	MS.gene91609:CDS	35.0%
	CAGTTCAATCCAAAAGATGA+CGG	-	chr7.4:85085753-85085772	MS.gene91609:CDS	35.0%
	CCAATTCCCGAAAACTATTT+CGG	-	chr7.4:85086485-85086504	MS.gene91609:CDS	35.0%
	GAAAGTAGAGATTTGAAAGG+TGG	-	chr7.4:85086803-85086822	MS.gene91609:CDS	35.0%
	GATTACTTCCCTACTCAAAA+AGG	+	chr7.4:85086090-85086109	None:intergenic	35.0%
	GCATAACCCTTCATGAAAAA+TGG	+	chr7.4:85086879-85086898	None:intergenic	35.0%
	GTGTGATTGTGAATTTGAGA+GGG	+	chr7.4:85085610-85085629	None:intergenic	35.0%
	GTTCTTGATAAGCATGTGAT+GGG	-	chr7.4:85086842-85086861	MS.gene91609:CDS	35.0%
	TATTTGGCTATGATTGGTGA+AGG	-	chr7.4:85086656-85086675	MS.gene91609:CDS	35.0%
	TGTGATTGTGAATTTGAGAG+GGG	+	chr7.4:85085609-85085628	None:intergenic	35.0%
	TGTTCTTGATAAGCATGTGA+TGG	-	chr7.4:85086841-85086860	MS.gene91609:CDS	35.0%
	TTCAAGTCTAAGAAAGTGAG+AGG	+	chr7.4:85085546-85085565	None:intergenic	35.0%
	TTCTGTTCCAACTATCTATG+AGG	+	chr7.4:85086139-85086158	None:intergenic	35.0%
	TTTGGATTGAACTGGATGAT+TGG	+	chr7.4:85085747-85085766	None:intergenic	35.0%
!	AAACAATGTTACCGGCTATA+GGG	+	chr7.4:85085704-85085723	None:intergenic	35.0%
!	ACAAAAGTGTGTTGTCCAAA+TGG	-	chr7.4:85086285-85086304	MS.gene91609:CDS	35.0%
!	ATGGATAAGGGTTTTCTTGA+TGG	-	chr7.4:85086611-85086630	MS.gene91609:CDS	35.0%
!	CTGCTCCACAATTTTATCAA+AGG	+	chr7.4:85085820-85085839	None:intergenic	35.0%
!	TGAAAGGTGGTGTTGAATTT+GGG	-	chr7.4:85086816-85086835	MS.gene91609:CDS	35.0%
!	TTGAAAGGTGGTGTTGAATT+TGG	-	chr7.4:85086815-85086834	MS.gene91609:CDS	35.0%
!!	AAATAGTTTTCGGGAATTGG+TGG	+	chr7.4:85086485-85086504	None:intergenic	35.0%
!!	CCAATGGAAAAACCACTTTT+CGG	+	chr7.4:85085942-85085961	None:intergenic	35.0%
!!	GAGAGTGAAGATTTGAGTTT+TGG	+	chr7.4:85085660-85085679	None:intergenic	35.0%
	AATCACTACTCCATCTTCCT+TGG	+	chr7.4:85086558-85086577	None:intergenic	40.0%
	ACACATCATGCTGTTCTTGA+TGG	-	chr7.4:85085969-85085988	MS.gene91609:CDS	40.0%
	AGAAGGCCAAACAATGTTAC+CGG	+	chr7.4:85085712-85085731	None:intergenic	40.0%
	AGGACACTCTCAAAACCATT+TGG	+	chr7.4:85086119-85086138	None:intergenic	40.0%
	ATTCAAGGAATAGGAGTTGC+AGG	-	chr7.4:85086680-85086699	MS.gene91609:CDS	40.0%
	CAACCATGTTCATCAAAGCT+TGG	-	chr7.4:85086000-85086019	MS.gene91609:CDS	40.0%
	CTATAGCCGGTAACATTGTT+TGG	-	chr7.4:85085703-85085722	MS.gene91609:CDS	40.0%
	GAAGACTTTACCAAGGAAGA+TGG	-	chr7.4:85086545-85086564	MS.gene91609:CDS	40.0%
	GGTGAAGGAATTCAAGGAAT+AGG	-	chr7.4:85086671-85086690	MS.gene91609:CDS	40.0%
	GGTGTGATTGTGAATTTGAG+AGG	+	chr7.4:85085611-85085630	None:intergenic	40.0%
	GTGATTGTGAATTTGAGAGG+GGG	+	chr7.4:85085608-85085627	None:intergenic	40.0%
	TCTTCCTTGGTAAAGTCTTC+TGG	+	chr7.4:85086545-85086564	None:intergenic	40.0%
	TTCAAGGAATAGGAGTTGCA+GGG	-	chr7.4:85086681-85086700	MS.gene91609:CDS	40.0%
!	AACGAAACTCCGTCATCTTT+TGG	+	chr7.4:85085765-85085784	None:intergenic	40.0%
!	AGAGTGTCCTCATAGATAGT+TGG	-	chr7.4:85086129-85086148	MS.gene91609:CDS	40.0%
!	CAAACAATGTTACCGGCTAT+AGG	+	chr7.4:85085705-85085724	None:intergenic	40.0%
!	CCGAAAAGTGGTTTTTCCAT+TGG	-	chr7.4:85085939-85085958	MS.gene91609:CDS	40.0%
!	CGAACGGAATCCTTAACTTT+TGG	+	chr7.4:85086224-85086243	None:intergenic	40.0%
!	CGTCATCTTTTGGATTGAAC+TGG	+	chr7.4:85085755-85085774	None:intergenic	40.0%
!!	CCGAAATAGTTTTCGGGAAT+TGG	+	chr7.4:85086488-85086507	None:intergenic	40.0%
!!	GAGACAACTTCAGTTGATAG+AGG	-	chr7.4:85086761-85086780	MS.gene91609:CDS	40.0%
!!	TATAGGGAGAAAGTGTTGGA+GGG	+	chr7.4:85085688-85085707	None:intergenic	40.0%
	CAACACTTTCTCCCTATAGC+CGG	-	chr7.4:85085690-85085709	MS.gene91609:CDS	45.0%
	TGAGCCAGAAGACTTTACCA+AGG	-	chr7.4:85086538-85086557	MS.gene91609:CDS	45.0%
	TTATGGAAGGCCTGCAAAAG+TGG	-	chr7.4:85086739-85086758	MS.gene91609:CDS	45.0%
!	GGACCAAGCTTTGATGAACA+TGG	+	chr7.4:85086006-85086025	None:intergenic	45.0%
!!	CTATAGGGAGAAAGTGTTGG+AGG	+	chr7.4:85085689-85085708	None:intergenic	45.0%
!!	GAAGTTGTCTCCACTTTTGC+AGG	+	chr7.4:85086752-85086771	None:intergenic	45.0%
	ATCTTCACGTGTGAGCTCGA+AGG	+	chr7.4:85086252-85086271	None:intergenic	50.0%
	CGAGCTCACACGTGAAGATT+TGG	-	chr7.4:85086253-85086272	MS.gene91609:CDS	50.0%
	GTTGCAGGGTCAACAAGGTT+TGG	-	chr7.4:85086695-85086714	MS.gene91609:CDS	50.0%
	TAGGAGTTGCAGGGTCAACA+AGG	-	chr7.4:85086690-85086709	MS.gene91609:CDS	50.0%
!	CGGCTATAGGGAGAAAGTGT+TGG	+	chr7.4:85085692-85085711	None:intergenic	50.0%
	GAGCTCGAAGGCGACTCGAA+CGG	+	chr7.4:85086240-85086259	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	85085465	85086907	85085465	ID=MS.gene91609
chr7.4	mRNA	85085465	85086907	85085465	ID=MS.gene91609.t1;Parent=MS.gene91609
chr7.4	exon	85085465	85086907	85085465	ID=MS.gene91609.t1.exon1;Parent=MS.gene91609.t1
chr7.4	CDS	85085465	85086907	85085465	ID=cds.MS.gene91609.t1;Parent=MS.gene91609.t1

Gene Sequence

>MS.gene91609

ATGGCTGCTGCAAACAATCACATCAAAATTCATGAAGAATGTAAAGTTTCTCCTAATTCTGCAAACCAATTATCTCTTCCTCTCACTTTCTTAGACTTGAATTGGCTAAGATTTCACCCAGTTGAACGTATTTTCTTCTACCCCCTCTCAAATTCACAATCACACCCTACTTTCTTCTTTCAAAACCTTGTTCCAAAACTCAAATCTTCACTCTCTTTAACCCTCCAACACTTTCTCCCTATAGCCGGTAACATTGTTTGGCCTTCTGATTCTTCAAAACCAATCATCCAGTTCAATCCAAAAGATGACGGAGTTTCGTTGCTTTTAGCGCAATATGATGACGATGTAGTTTCCTTTGATAAAATTGTGGAGCAGAATTCAACACAAGAAGCTTCTTTATCTCGTTCTTTTGTACCTCACTTAGAATCAATAGATTCTTTTGCTTCTATCATTTCAATTCAGATAACTCTTTTTCCGAAAAGTGGTTTTTCCATTGGAATAAGCACACATCATGCTGTTCTTGATGGAAAATCTTCAACCATGTTCATCAAAGCTTGGTCCTTTATATGTAAATCTTTTGAAGATGAAACACAATCATCAAATTTAGAGCCTTTTTTGAAACCCTTTTTGAGTAGGGAAGTAATCAATGATCCAAATGGTTTTGAGAGTGTCCTCATAGATAGTTGGAACAGAATCTCAACACATTTTGACAAAGGCAACGTGAGAAGCTTAAAGATTATGTCAAATTTCCTTGAACCAAAAGTTAAGGATTCCGTTCGAGTCGCCTTCGAGCTCACACGTGAAGATTTGGAGAAATTAAACAAAAGTGTGTTGTCCAAATGGAACAATATTGAAGAAGGATCACAAGAACAAGAACAACCAAAGAAATTGTCAACTTTTGTTCTTACATGTGCTTATGTTTCCGTTTGCATAGCAAAAGCAAAACAACAATCTGAAAGCAATCAAAAACAAAAATTTTGTTTTGGTTTTGCAGTGGATATCAGATCAAGATTAGATCCACCAATTCCCGAAAACTATTTCGGAAATTGTGTAACTAATCATATAATTGATACTGAGCCAGAAGACTTTACCAAGGAAGATGGAGTAGTGATTTTTGCAAAGAAAATTTATAGCAAAATTAGAAAAATGGATAAGGGTTTTCTTGATGGTGTAGATACATTGTTGTCTAAATATTTGGCTATGATTGGTGAAGGAATTCAAGGAATAGGAGTTGCAGGGTCAACAAGGTTTGGAGTATATGAGATTGATTTTGGTTATGGAAGGCCTGCAAAAGTGGAGACAACTTCAGTTGATAGAGGAATAACAATTGGATTAACAGAAAGTAGAGATTTGAAAGGTGGTGTTGAATTTGGGCTTGTTCTTGATAAGCATGTGATGGGTTTTTTTCATACCATTTTTCATGAAGGGTTATGCTTTGATTGA

Protein sequence

>MS.gene91609.t1

MAAANNHIKIHEECKVSPNSANQLSLPLTFLDLNWLRFHPVERIFFYPLSNSQSHPTFFFQNLVPKLKSSLSLTLQHFLPIAGNIVWPSDSSKPIIQFNPKDDGVSLLLAQYDDDVVSFDKIVEQNSTQEASLSRSFVPHLESIDSFASIISIQITLFPKSGFSIGISTHHAVLDGKSSTMFIKAWSFICKSFEDETQSSNLEPFLKPFLSREVINDPNGFESVLIDSWNRISTHFDKGNVRSLKIMSNFLEPKVKDSVRVAFELTREDLEKLNKSVLSKWNNIEEGSQEQEQPKKLSTFVLTCAYVSVCIAKAKQQSESNQKQKFCFGFAVDIRSRLDPPIPENYFGNCVTNHIIDTEPEDFTKEDGVVIFAKKIYSKIRKMDKGFLDGVDTLLSKYLAMIGEGIQGIGVAGSTRFGVYEIDFGYGRPAKVETTSVDRGITIGLTESRDLKGGVEFGLVLDKHVMGFFHTIFHEGLCFD