AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene043352

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043352.t1 MTR_2g089765 92.798 486 35 0 7 492 3 488 0.0 943
MS.gene043352.t1 MTR_2g089670 70.968 496 121 7 6 492 2 483 0.0 667
MS.gene043352.t1 MTR_8g012750 69.008 484 131 11 18 490 1 476 0.0 624
MS.gene043352.t1 MTR_2g089665 60.360 444 64 6 20 461 2 335 1.29e-173 492
MS.gene043352.t1 MTR_7g015860 43.154 482 233 12 20 486 5 460 8.22e-119 357
MS.gene043352.t1 MTR_3g028210 36.157 484 275 10 18 489 6 467 9.52e-99 305
MS.gene043352.t1 MTR_7g014330 37.924 472 261 12 21 482 3 452 1.25e-88 279
MS.gene043352.t1 MTR_2g089650 36.214 486 274 13 16 489 5 466 2.13e-88 278
MS.gene043352.t1 MTR_6g015850 36.401 489 266 15 14 489 4 460 1.96e-83 265
MS.gene043352.t1 MTR_2g089755 36.226 461 261 12 43 489 37 478 1.09e-82 264
MS.gene043352.t1 MTR_7g014230 36.864 472 266 12 21 482 5 454 2.09e-81 260
MS.gene043352.t1 MTR_6g015815 35.343 481 273 12 21 489 9 463 3.49e-81 259
MS.gene043352.t1 MTR_7g014250 36.017 472 270 12 21 482 3 452 5.78e-80 256
MS.gene043352.t1 MTR_2g089735 35.808 458 256 14 47 489 30 464 6.55e-78 251
MS.gene043352.t1 MTR_6g015830 35.510 490 272 15 14 489 4 463 1.33e-77 250
MS.gene043352.t1 MTR_7g014200 34.921 504 271 17 6 488 3 470 2.04e-77 250
MS.gene043352.t1 MTR_3g087640 35.113 487 273 14 21 487 10 473 2.07e-77 250
MS.gene043352.t1 MTR_6g015320 32.700 474 283 11 25 489 11 457 9.58e-73 238
MS.gene043352.t1 MTR_7g014160 33.665 502 281 16 6 486 3 473 7.56e-72 236
MS.gene043352.t1 MTR_7g014610 33.728 507 270 17 17 491 5 477 4.46e-69 228
MS.gene043352.t1 MTR_6g015315 32.589 448 275 7 47 489 30 455 5.91e-68 225
MS.gene043352.t1 MTR_7g013850 31.712 473 271 13 44 486 30 480 3.10e-62 211
MS.gene043352.t1 MTR_7g014620 33.477 463 252 12 39 486 11 432 1.71e-58 199
MS.gene043352.t1 MTR_7g014360 31.514 403 225 13 89 482 22 382 9.62e-46 164
MS.gene043352.t1 MTR_3g435300 36.170 282 156 8 21 292 4 271 5.34e-43 154
MS.gene043352.t1 MTR_2g089745 89.157 83 9 0 410 492 1 83 7.21e-42 144
MS.gene043352.t1 MTR_7g014630 34.589 292 154 10 17 289 6 279 4.06e-33 129
MS.gene043352.t1 MTR_7g014310 29.942 344 172 10 127 468 29 305 5.21e-31 122
MS.gene043352.t1 MTR_6g015310 27.411 394 202 11 85 464 10 333 6.17e-31 122
MS.gene043352.t1 MTR_7g014670 37.037 189 110 2 299 486 79 259 1.33e-30 120
MS.gene043352.t1 MTR_7g014180 38.764 178 95 5 20 193 7 174 2.61e-26 105
MS.gene043352.t1 MTR_8g041770 26.098 410 240 12 43 438 30 390 1.52e-18 88.2
MS.gene043352.t1 MTR_3g098980 24.331 411 254 12 78 478 65 428 1.55e-18 88.2
MS.gene043352.t1 MTR_7g026860 24.619 394 243 13 57 438 38 389 1.12e-17 85.5
MS.gene043352.t1 MTR_4g022825 25.060 415 237 15 49 438 26 391 1.17e-16 82.4
MS.gene043352.t1 MTR_4g022720 24.138 435 264 14 19 438 3 386 1.37e-16 82.4
MS.gene043352.t1 MTR_8g096530 24.779 452 268 19 53 490 36 429 1.47e-16 82.4
MS.gene043352.t1 MTR_7g014210 39.130 115 68 2 344 456 24 138 1.55e-16 77.4
MS.gene043352.t1 MTR_3g070170 21.915 470 294 16 41 478 31 459 1.82e-15 79.0
MS.gene043352.t1 MTR_4g022670 23.632 402 268 12 43 438 24 392 2.27e-15 78.6
MS.gene043352.t1 MTR_4g022800 23.971 413 254 15 43 438 24 393 3.73e-15 77.8
MS.gene043352.t1 MTR_7g014340 58.824 68 27 1 21 87 3 70 4.51e-15 71.2
MS.gene043352.t1 MTR_0190s0020 25.296 423 257 13 64 471 48 426 4.71e-15 77.8
MS.gene043352.t1 MTR_4g127650 23.909 481 278 15 41 486 29 456 8.74e-15 77.0
MS.gene043352.t1 MTR_7g014220 58.730 63 26 0 35 97 10 72 3.65e-14 68.2
MS.gene043352.t1 MTR_3g026310 23.430 414 261 13 43 438 24 399 7.15e-14 73.9
MS.gene043352.t1 MTR_2g064495 21.689 438 286 13 59 484 39 431 7.26e-14 73.9
MS.gene043352.t1 MTR_7g014205 37.719 114 59 2 342 454 18 120 8.81e-14 68.2
MS.gene043352.t1 MTR_4g094828 21.103 417 269 13 39 438 25 398 1.54e-13 72.8
MS.gene043352.t1 MTR_8g075610 24.018 433 267 17 59 478 30 413 2.21e-13 72.4
MS.gene043352.t1 MTR_3g435290 33.884 121 77 2 367 487 1 118 2.23e-13 67.0
MS.gene043352.t1 MTR_4g097090 23.944 426 248 15 41 437 28 406 2.93e-12 68.9
MS.gene043352.t1 MTR_3g101900 22.391 460 291 17 43 480 30 445 5.87e-12 68.2
MS.gene043352.t1 MTR_3g101960 23.093 485 292 21 19 475 5 436 1.13e-11 67.0
MS.gene043352.t1 MTR_3g049150 26.059 307 167 11 159 438 126 399 1.72e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043352.t1 AT1G03940 38.958 480 265 12 21 491 9 469 1.40e-104 320
MS.gene043352.t1 AT1G03495 39.888 356 182 12 22 368 10 342 1.38e-70 228
MS.gene043352.t1 AT3G29670 32.231 484 278 15 18 486 1 449 1.76e-65 218
MS.gene043352.t1 AT5G61160 31.967 488 279 16 18 488 1 452 4.11e-62 209
MS.gene043352.t1 AT3G29635 30.738 488 291 13 18 489 1 457 1.37e-59 203
MS.gene043352.t1 AT3G29590 32.359 479 278 16 19 487 7 449 2.45e-57 197
MS.gene043352.t1 AT3G29680 30.252 476 294 10 20 487 3 448 3.03e-56 194
MS.gene043352.t1 AT5G39090 30.042 476 293 13 19 486 4 447 1.38e-55 192
MS.gene043352.t1 AT5G39050 29.719 498 299 13 12 488 2 469 3.00e-55 192
MS.gene043352.t1 AT5G39080 27.721 487 304 15 19 486 4 461 4.38e-49 175
MS.gene043352.t1 AT3G48720 22.936 436 271 13 56 478 41 424 1.90e-18 88.2
MS.gene043352.t1 AT3G29690 31.481 162 99 4 331 488 27 180 2.20e-16 77.8
MS.gene043352.t1 AT5G41040 23.598 428 267 14 73 489 63 441 8.27e-16 80.1
MS.gene043352.t1 AT5G41040 23.598 428 267 14 73 489 79 457 1.23e-15 79.7
MS.gene043352.t1 AT1G65450 24.776 335 207 13 163 486 149 449 3.48e-13 72.0

Find 105 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCACTGTTCTTCCAAATTC+TGG 0.075389 2.2:+10165975 MS.gene043352:CDS
TTCATCATTTCATGAAATTC+TGG 0.154366 2.2:+10166048 MS.gene043352:CDS
GGAGGGGTGTTAAGGGAATT+TGG 0.209569 2.2:-10165771 None:intergenic
CAAATAGACAACCCAGAATT+TGG 0.217457 2.2:-10165987 None:intergenic
AAACATTGATGTCTGATTAT+AGG 0.245779 2.2:+10166672 MS.gene043352:CDS
GGTTGCTGGTTCACCGAAAT+TGG 0.246480 2.2:+10166727 MS.gene043352:CDS
TTGAAAGATGTTAATGAAAT+TGG 0.248470 2.2:-10166897 None:intergenic
GTACGAGGTGGAGGTAAATT+TGG 0.264562 2.2:-10165909 None:intergenic
GCTGTTTATGAAACTGATTT+TGG 0.276498 2.2:+10166749 MS.gene043352:CDS
CATCCCTATAATGGCTATTC+AGG 0.277699 2.2:+10165953 MS.gene043352:CDS
TAACCCATTTCTTCAACTTC+TGG 0.280416 2.2:-10166307 None:intergenic
AACATTGATGTCTGATTATA+GGG 0.290004 2.2:+10166673 MS.gene043352:CDS
GTTGGTGATGGAACTGAATT+TGG 0.302858 2.2:-10165564 None:intergenic
GTAAAGAGAGATGAGTTAAT+TGG 0.307415 2.2:+10166569 MS.gene043352:CDS
TTTAAGAGTGATGCTTTGTT+AGG 0.320839 2.2:+10166644 MS.gene043352:CDS
CTTTAACTCTATCTCTTTCA+TGG 0.321227 2.2:-10166148 None:intergenic
GTGACCTGAATAGCCATTAT+AGG 0.322860 2.2:-10165957 None:intergenic
ATGATGAGGTTGGAGGAAAA+AGG 0.329263 2.2:-10165744 None:intergenic
TCACTGTTCTTCCAAATTCT+GGG 0.346380 2.2:+10165976 MS.gene043352:CDS
TGACATGGAATTCGCGGGTT+TGG 0.346535 2.2:+10166226 MS.gene043352:CDS
GTATATAAGGAGGGGTGTTA+AGG 0.354241 2.2:-10165779 None:intergenic
ACTATGTTCTATCACCTTCA+TGG 0.354613 2.2:-10165526 None:intergenic
TTGTAACAAGCTCCTTGATT+TGG 0.359633 2.2:+10166378 MS.gene043352:CDS
TAGAAGAAGATACGTTTAAC+AGG 0.362847 2.2:-10165633 None:intergenic
TGAACTGCATCAGATTTCTT+AGG 0.374068 2.2:-10166782 None:intergenic
AAGTCCAGAAGTTGAAGAAA+TGG 0.376086 2.2:+10166303 MS.gene043352:CDS
GTTTCATAAACAGCCAATTT+CGG 0.380067 2.2:-10166740 None:intergenic
TCATCATTTCATGAAATTCT+GGG 0.381535 2.2:+10166049 MS.gene043352:CDS
TGTGTTTGTGTTTGTGTTTG+TGG 0.385306 2.2:-10165478 None:intergenic
CATTCGTGTCATCCCTATAA+TGG 0.385956 2.2:+10165944 MS.gene043352:CDS
GAAGAAGTGTTGGAGGGTTA+AGG 0.387404 2.2:-10165721 None:intergenic
GGAAAGTGAATGTTTAAGAA+TGG 0.394305 2.2:-10165700 None:intergenic
ATTATTGTTGTTTGTTGCTA+TGG 0.405288 2.2:-10166087 None:intergenic
ATTCACAAGATGCTCAAAAC+TGG 0.405633 2.2:+10165874 MS.gene043352:CDS
AAGTCGGTTACTGTGGTTGC+TGG 0.409781 2.2:+10166713 MS.gene043352:CDS
TCATCAAGATAACGTATATA+AGG 0.410087 2.2:-10165792 None:intergenic
ATTTGGAGGGATGATGAGGT+TGG 0.424629 2.2:-10165754 None:intergenic
TGACCTGAATAGCCATTATA+GGG 0.424676 2.2:-10165956 None:intergenic
TATATAAGGAGGGGTGTTAA+GGG 0.438164 2.2:-10165778 None:intergenic
TCTCTTTCTGATTGTAGAGA+TGG 0.439448 2.2:+10166827 MS.gene043352:CDS
AGATAGAGTTAAAGATCTAA+AGG 0.440490 2.2:+10166157 MS.gene043352:CDS
TGGTATGTCGAAGAAAGTTA+AGG 0.445120 2.2:-10165595 None:intergenic
TGGTGGAATTGAAGTTGGTT+TGG 0.445540 2.2:+10166853 MS.gene043352:CDS
TATCACCTTCATGGTGAACT+TGG 0.449377 2.2:-10165517 None:intergenic
GAAAGTGAATGTTTAAGAAT+GGG 0.454014 2.2:-10165699 None:intergenic
GCAGTTCATCTTGATTCATC+CGG 0.454263 2.2:+10166797 MS.gene043352:CDS
GGTGGTGGTGGAATTGAAGT+TGG 0.467228 2.2:+10166848 MS.gene043352:CDS
GAATGGGAAGTGTTGTTTGA+AGG 0.475295 2.2:-10165683 None:intergenic
GGGATTGTTGTGGCTTCAAT+TGG 0.477890 2.2:+10166599 MS.gene043352:CDS
GATAGAGTTAAAGATCTAAA+GGG 0.478327 2.2:+10166158 MS.gene043352:CDS
GGGAATTTGGAGGGATGATG+AGG 0.481946 2.2:-10165758 None:intergenic
GGTATGTCGAAGAAAGTTAA+GGG 0.489263 2.2:-10165594 None:intergenic
GGTGATGGAACTGAATTTGG+TGG 0.489909 2.2:-10165561 None:intergenic
AGTCCAGAAGTTGAAGAAAT+GGG 0.492194 2.2:+10166304 MS.gene043352:CDS
GTTTGAAGGAAGTGATGAGT+TGG 0.500560 2.2:-10165669 None:intergenic
TTTAACTCTATCTCTTTCAT+GGG 0.506937 2.2:-10166147 None:intergenic
TGGAGGGATGATGAGGTTGG+AGG 0.507278 2.2:-10165751 None:intergenic
AAGGGGAGAATGGTTGGTGA+TGG 0.521926 2.2:-10165576 None:intergenic
AAGTTGGTTTGGCTCTTGAG+AGG 0.523983 2.2:+10166864 MS.gene043352:CDS
CTTTCTGATTGTAGAGATGG+TGG 0.524087 2.2:+10166830 MS.gene043352:CDS
ATCAGAAAGAGAAATCGAAC+CGG 0.526033 2.2:-10166816 None:intergenic
GATCATACAAAACCAAATCA+AGG 0.527811 2.2:-10166390 None:intergenic
ATCAAATGACATGGAATTCG+CGG 0.527943 2.2:+10166220 MS.gene043352:CDS
GATTGTAGAGATGGTGGTGG+TGG 0.534276 2.2:+10166836 MS.gene043352:CDS
ACTCTATCTCTTTCATGGGA+AGG 0.547296 2.2:-10166143 None:intergenic
AAAGTTAAGGGGAGAATGGT+TGG 0.550742 2.2:-10165582 None:intergenic
TTGTCTTGCATCTTATATTG+TGG 0.552776 2.2:+10166544 MS.gene043352:CDS
TCTTGTTCAGTACGAGGTGG+AGG 0.553828 2.2:-10165918 None:intergenic
TCAAATGACATGGAATTCGC+GGG 0.559911 2.2:+10166221 MS.gene043352:CDS
GAAGAAAGTTAAGGGGAGAA+TGG 0.561998 2.2:-10165586 None:intergenic
ACCACAGTAACCGACTTACC+AGG 0.562319 2.2:-10166707 None:intergenic
GATGAGTTGGATGAGGAAAG+TGG 0.563879 2.2:-10165656 None:intergenic
CAAGATAACGTATATAAGGA+GGG 0.570632 2.2:-10165788 None:intergenic
TGAGGATTCAGCGACGGTGA+AGG 0.579651 2.2:-10165826 None:intergenic
GGGGTGTTAAGGGAATTTGG+AGG 0.579772 2.2:-10165768 None:intergenic
CATGGGAAGGAAGATCAAGA+GGG 0.580759 2.2:-10166130 None:intergenic
TCAAGATAACGTATATAAGG+AGG 0.581922 2.2:-10165789 None:intergenic
GATGGAACTGAATTTGGTGG+TGG 0.582071 2.2:-10165558 None:intergenic
TACAACCATCTTGCTGCAGA+CGG 0.585188 2.2:+10166017 MS.gene043352:CDS
GAGGTAAATTTGGAACAAGA+TGG 0.586717 2.2:-10165899 None:intergenic
TAACTTTCTTCGACATACCA+TGG 0.593348 2.2:+10165598 MS.gene043352:CDS
AATATCAAAACCTGGTAAGT+CGG 0.594383 2.2:+10166697 MS.gene043352:CDS
CTTGCAGATTGTCGTGATAG+CGG 0.594957 2.2:+10166488 MS.gene043352:CDS
TTGTATGATCAAATCAGAGG+AGG 0.597606 2.2:+10166403 MS.gene043352:CDS
ACCTGGTAAGTCGGTTACTG+TGG 0.598866 2.2:+10166706 MS.gene043352:CDS
TGAGACCAAGTTCACCATGA+AGG 0.600163 2.2:+10165512 MS.gene043352:CDS
AGGAAGTGATGAGTTGGATG+AGG 0.606178 2.2:-10165663 None:intergenic
GGGTGTTAAGGGAATTTGGA+GGG 0.611740 2.2:-10165767 None:intergenic
TGTATGATCAAATCAGAGGA+GGG 0.615042 2.2:+10166404 MS.gene043352:CDS
AAAAGGGAAGAAGTGTTGGA+GGG 0.615109 2.2:-10165727 None:intergenic
GTCTGCTGAGGATTCAGCGA+CGG 0.615979 2.2:-10165832 None:intergenic
TCTGATTGTAGAGATGGTGG+TGG 0.621226 2.2:+10166833 MS.gene043352:CDS
TATAGGGAAATATCAAAACC+TGG 0.626368 2.2:+10166689 MS.gene043352:CDS
TCATGGGAAGGAAGATCAAG+AGG 0.635606 2.2:-10166131 None:intergenic
AGATTCTGAATCAAATGACA+TGG 0.642714 2.2:+10166211 MS.gene043352:CDS
TTGTCTTGTTCAGTACGAGG+TGG 0.652618 2.2:-10165921 None:intergenic
GTATGTCGAAGAAAGTTAAG+GGG 0.670105 2.2:-10165593 None:intergenic
GAGGATGTTGAAGTCTGCTG+AGG 0.672509 2.2:-10165844 None:intergenic
AAGATAACGTATATAAGGAG+GGG 0.677863 2.2:-10165787 None:intergenic
CATCTTGTGAATCAGATACG+AGG 0.681815 2.2:-10165863 None:intergenic
ATGTTGTCTTGTTCAGTACG+AGG 0.683962 2.2:-10165924 None:intergenic
ACAGGGTGACAATAAAACCA+TGG 0.687760 2.2:-10165615 None:intergenic
AGAAGAAGATACGTTTAACA+GGG 0.696018 2.2:-10165632 None:intergenic
TGGGAAGGAAGATCAAGAGG+GGG 0.698434 2.2:-10166128 None:intergenic
ATGGGAAGGAAGATCAAGAG+GGG 0.712513 2.2:-10166129 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAGTTAATTGGAAAAAAT+GGG + chr2.2:10166579-10166598 MS.gene043352:CDS 20.0%
!! TTGAAAGATGTTAATGAAAT+TGG - chr2.2:10166900-10166919 None:intergenic 20.0%
!!! CAATTTCCAAAATAAGTTTT+TGG - chr2.2:10166527-10166546 None:intergenic 20.0%
!!! TCATTACCAAAAACTTATTT+TGG + chr2.2:10166518-10166537 MS.gene043352:CDS 20.0%
! AAACATTGATGTCTGATTAT+AGG + chr2.2:10166672-10166691 MS.gene043352:CDS 25.0%
! AACATTGATGTCTGATTATA+GGG + chr2.2:10166673-10166692 MS.gene043352:CDS 25.0%
! AGATAGAGTTAAAGATCTAA+AGG + chr2.2:10166157-10166176 MS.gene043352:CDS 25.0%
! GAAAGTGAATGTTTAAGAAT+GGG - chr2.2:10165702-10165721 None:intergenic 25.0%
! GATAGAGTTAAAGATCTAAA+GGG + chr2.2:10166158-10166177 MS.gene043352:CDS 25.0%
! GATGAGTTAATTGGAAAAAA+TGG + chr2.2:10166578-10166597 MS.gene043352:CDS 25.0%
! TCATCAAGATAACGTATATA+AGG - chr2.2:10165795-10165814 None:intergenic 25.0%
! TCATCATTTCATGAAATTCT+GGG + chr2.2:10166049-10166068 MS.gene043352:CDS 25.0%
! TTCATCATTTCATGAAATTC+TGG + chr2.2:10166048-10166067 MS.gene043352:CDS 25.0%
! TTTAACTCTATCTCTTTCAT+GGG - chr2.2:10166150-10166169 None:intergenic 25.0%
!!! ATTATTGTTGTTTGTTGCTA+TGG - chr2.2:10166090-10166109 None:intergenic 25.0%
!!! CTGTTTATGAAACTGATTTT+GGG + chr2.2:10166750-10166769 MS.gene043352:CDS 25.0%
AAGATAACGTATATAAGGAG+GGG - chr2.2:10165790-10165809 None:intergenic 30.0%
AATATCAAAACCTGGTAAGT+CGG + chr2.2:10166697-10166716 MS.gene043352:CDS 30.0%
AGAAGAAGATACGTTTAACA+GGG - chr2.2:10165635-10165654 None:intergenic 30.0%
AGATTCTGAATCAAATGACA+TGG + chr2.2:10166211-10166230 MS.gene043352:CDS 30.0%
CAAGATAACGTATATAAGGA+GGG - chr2.2:10165791-10165810 None:intergenic 30.0%
CTTTAACTCTATCTCTTTCA+TGG - chr2.2:10166151-10166170 None:intergenic 30.0%
GATCATACAAAACCAAATCA+AGG - chr2.2:10166393-10166412 None:intergenic 30.0%
GGAAAGTGAATGTTTAAGAA+TGG - chr2.2:10165703-10165722 None:intergenic 30.0%
GGTGATAAAAAAAGTGATCA+AGG + chr2.2:10166425-10166444 MS.gene043352:CDS 30.0%
GTAAAGAGAGATGAGTTAAT+TGG + chr2.2:10166569-10166588 MS.gene043352:CDS 30.0%
GTTTCATAAACAGCCAATTT+CGG - chr2.2:10166743-10166762 None:intergenic 30.0%
TAGAAGAAGATACGTTTAAC+AGG - chr2.2:10165636-10165655 None:intergenic 30.0%
TATAGGGAAATATCAAAACC+TGG + chr2.2:10166689-10166708 MS.gene043352:CDS 30.0%
TCAAGATAACGTATATAAGG+AGG - chr2.2:10165792-10165811 None:intergenic 30.0%
TTGTCTTGCATCTTATATTG+TGG + chr2.2:10166544-10166563 MS.gene043352:CDS 30.0%
! GTTTTGTATGATCAAATCAG+AGG + chr2.2:10166400-10166419 MS.gene043352:CDS 30.0%
! TTTAAGAGTGATGCTTTGTT+AGG + chr2.2:10166644-10166663 MS.gene043352:CDS 30.0%
!! GCTGTTTATGAAACTGATTT+TGG + chr2.2:10166749-10166768 MS.gene043352:CDS 30.0%
!! TTATGAAACTGATTTTGGGT+GGG + chr2.2:10166754-10166773 MS.gene043352:CDS 30.0%
!! TTTATGAAACTGATTTTGGG+TGG + chr2.2:10166753-10166772 MS.gene043352:CDS 30.0%
AAGTCCAGAAGTTGAAGAAA+TGG + chr2.2:10166303-10166322 MS.gene043352:CDS 35.0%
ACTATGTTCTATCACCTTCA+TGG - chr2.2:10165529-10165548 None:intergenic 35.0%
AGTCCAGAAGTTGAAGAAAT+GGG + chr2.2:10166304-10166323 MS.gene043352:CDS 35.0%
ATCAAATGACATGGAATTCG+CGG + chr2.2:10166220-10166239 MS.gene043352:CDS 35.0%
ATCAGAAAGAGAAATCGAAC+CGG - chr2.2:10166819-10166838 None:intergenic 35.0%
ATTCACAAGATGCTCAAAAC+TGG + chr2.2:10165874-10165893 MS.gene043352:CDS 35.0%
CAAATAGACAACCCAGAATT+TGG - chr2.2:10165990-10166009 None:intergenic 35.0%
GGTATGTCGAAGAAAGTTAA+GGG - chr2.2:10165597-10165616 None:intergenic 35.0%
GTATGTCGAAGAAAGTTAAG+GGG - chr2.2:10165596-10165615 None:intergenic 35.0%
TAACCCATTTCTTCAACTTC+TGG - chr2.2:10166310-10166329 None:intergenic 35.0%
TAACTTTCTTCGACATACCA+TGG + chr2.2:10165598-10165617 MS.gene043352:CDS 35.0%
TCACTGTTCTTCCAAATTCT+GGG + chr2.2:10165976-10165995 MS.gene043352:CDS 35.0%
TCTCTTTCTGATTGTAGAGA+TGG + chr2.2:10166827-10166846 MS.gene043352:CDS 35.0%
TGAACTGCATCAGATTTCTT+AGG - chr2.2:10166785-10166804 None:intergenic 35.0%
TGACCTGAATAGCCATTATA+GGG - chr2.2:10165959-10165978 None:intergenic 35.0%
TGGAAAAAATGGGATTGTTG+TGG + chr2.2:10166589-10166608 MS.gene043352:CDS 35.0%
TGGTATGTCGAAGAAAGTTA+AGG - chr2.2:10165598-10165617 None:intergenic 35.0%
TGTATGATCAAATCAGAGGA+GGG + chr2.2:10166404-10166423 MS.gene043352:CDS 35.0%
TGTGTTTGTGTTTGTGTTTG+TGG - chr2.2:10165481-10165500 None:intergenic 35.0%
TTGTAACAAGCTCCTTGATT+TGG + chr2.2:10166378-10166397 MS.gene043352:CDS 35.0%
TTGTATGATCAAATCAGAGG+AGG + chr2.2:10166403-10166422 MS.gene043352:CDS 35.0%
! GAGGTAAATTTGGAACAAGA+TGG - chr2.2:10165902-10165921 None:intergenic 35.0%
!! TATATAAGGAGGGGTGTTAA+GGG - chr2.2:10165781-10165800 None:intergenic 35.0%
!! TATGAAACTGATTTTGGGTG+GGG + chr2.2:10166755-10166774 MS.gene043352:CDS 35.0%
AAAGTTAAGGGGAGAATGGT+TGG - chr2.2:10165585-10165604 None:intergenic 40.0%
ACAGGGTGACAATAAAACCA+TGG - chr2.2:10165618-10165637 None:intergenic 40.0%
ACTCTATCTCTTTCATGGGA+AGG - chr2.2:10166146-10166165 None:intergenic 40.0%
ATGATGAGGTTGGAGGAAAA+AGG - chr2.2:10165747-10165766 None:intergenic 40.0%
CATCCCTATAATGGCTATTC+AGG + chr2.2:10165953-10165972 MS.gene043352:CDS 40.0%
CATCTTGTGAATCAGATACG+AGG - chr2.2:10165866-10165885 None:intergenic 40.0%
CATTCGTGTCATCCCTATAA+TGG + chr2.2:10165944-10165963 MS.gene043352:CDS 40.0%
CTTTCTGATTGTAGAGATGG+TGG + chr2.2:10166830-10166849 MS.gene043352:CDS 40.0%
GAAGAAAGTTAAGGGGAGAA+TGG - chr2.2:10165589-10165608 None:intergenic 40.0%
GCAGTTCATCTTGATTCATC+CGG + chr2.2:10166797-10166816 MS.gene043352:CDS 40.0%
GTCACTGTTCTTCCAAATTC+TGG + chr2.2:10165975-10165994 MS.gene043352:CDS 40.0%
GTGACCTGAATAGCCATTAT+AGG - chr2.2:10165960-10165979 None:intergenic 40.0%
GTTTGAAGGAAGTGATGAGT+TGG - chr2.2:10165672-10165691 None:intergenic 40.0%
TATCACCTTCATGGTGAACT+TGG - chr2.2:10165520-10165539 None:intergenic 40.0%
TCAAATGACATGGAATTCGC+GGG + chr2.2:10166221-10166240 MS.gene043352:CDS 40.0%
TGATGAGGTTGGAGGAAAAA+GGG - chr2.2:10165746-10165765 None:intergenic 40.0%
! AGGAAAAAGGGAAGAAGTGT+TGG - chr2.2:10165734-10165753 None:intergenic 40.0%
! ATGTTGTCTTGTTCAGTACG+AGG - chr2.2:10165927-10165946 None:intergenic 40.0%
! TGGTGGAATTGAAGTTGGTT+TGG + chr2.2:10166853-10166872 MS.gene043352:CDS 40.0%
!! AAAAAGGGAAGAAGTGTTGG+AGG - chr2.2:10165731-10165750 None:intergenic 40.0%
!! AAAAGGGAAGAAGTGTTGGA+GGG - chr2.2:10165730-10165749 None:intergenic 40.0%
!! ATGAAACTGATTTTGGGTGG+GGG + chr2.2:10166756-10166775 MS.gene043352:CDS 40.0%
!! GAATGGGAAGTGTTGTTTGA+AGG - chr2.2:10165686-10165705 None:intergenic 40.0%
!! GTATATAAGGAGGGGTGTTA+AGG - chr2.2:10165782-10165801 None:intergenic 40.0%
!! GTTGGTGATGGAACTGAATT+TGG - chr2.2:10165567-10165586 None:intergenic 40.0%
!! TGCTATGGCTTTTGAAAGAG+AGG - chr2.2:10166075-10166094 None:intergenic 40.0%
ATGGGAAGGAAGATCAAGAG+GGG - chr2.2:10166132-10166151 None:intergenic 45.0%
ATTTGGAGGGATGATGAGGT+TGG - chr2.2:10165757-10165776 None:intergenic 45.0%
CATGGGAAGGAAGATCAAGA+GGG - chr2.2:10166133-10166152 None:intergenic 45.0%
CTTGCAGATTGTCGTGATAG+CGG + chr2.2:10166488-10166507 MS.gene043352:CDS 45.0%
GGGATTGTTGTGGCTTCAAT+TGG + chr2.2:10166599-10166618 MS.gene043352:CDS 45.0%
GGGTGTTAAGGGAATTTGGA+GGG - chr2.2:10165770-10165789 None:intergenic 45.0%
GTACGAGGTGGAGGTAAATT+TGG - chr2.2:10165912-10165931 None:intergenic 45.0%
TACAACCATCTTGCTGCAGA+CGG + chr2.2:10166017-10166036 MS.gene043352:CDS 45.0%
TCATGGGAAGGAAGATCAAG+AGG - chr2.2:10166134-10166153 None:intergenic 45.0%
TGAGACCAAGTTCACCATGA+AGG + chr2.2:10165512-10165531 MS.gene043352:CDS 45.0%
! AGGAAGTGATGAGTTGGATG+AGG - chr2.2:10165666-10165685 None:intergenic 45.0%
! ATTTTCCGTCTGCAGCAAGA+TGG - chr2.2:10166025-10166044 None:intergenic 45.0%
! GATGAGTTGGATGAGGAAAG+TGG - chr2.2:10165659-10165678 None:intergenic 45.0%
! GATGGAACTGAATTTGGTGG+TGG - chr2.2:10165561-10165580 None:intergenic 45.0%
! TCTGATTGTAGAGATGGTGG+TGG + chr2.2:10166833-10166852 MS.gene043352:CDS 45.0%
! TTGTCTTGTTCAGTACGAGG+TGG - chr2.2:10165924-10165943 None:intergenic 45.0%
!! AAGTTGGTTTGGCTCTTGAG+AGG + chr2.2:10166864-10166883 MS.gene043352:CDS 45.0%
!! GAAGAAGTGTTGGAGGGTTA+AGG - chr2.2:10165724-10165743 None:intergenic 45.0%
!! GGTGATGGAACTGAATTTGG+TGG - chr2.2:10165564-10165583 None:intergenic 45.0%
AAGTCGGTTACTGTGGTTGC+TGG + chr2.2:10166713-10166732 MS.gene043352:CDS 50.0%
ACCTGGTAAGTCGGTTACTG+TGG + chr2.2:10166706-10166725 MS.gene043352:CDS 50.0%
GAGGATGTTGAAGTCTGCTG+AGG - chr2.2:10165847-10165866 None:intergenic 50.0%
GGGAATTTGGAGGGATGATG+AGG - chr2.2:10165761-10165780 None:intergenic 50.0%
TCTTGTTCAGTACGAGGTGG+AGG - chr2.2:10165921-10165940 None:intergenic 50.0%
TGACATGGAATTCGCGGGTT+TGG + chr2.2:10166226-10166245 MS.gene043352:CDS 50.0%
TGGGAAGGAAGATCAAGAGG+GGG - chr2.2:10166131-10166150 None:intergenic 50.0%
! ACCACAGTAACCGACTTACC+AGG - chr2.2:10166710-10166729 None:intergenic 50.0%
! GGGGTGTTAAGGGAATTTGG+AGG - chr2.2:10165771-10165790 None:intergenic 50.0%
!! AAGGGGAGAATGGTTGGTGA+TGG - chr2.2:10165579-10165598 None:intergenic 50.0%
!! GATTGTAGAGATGGTGGTGG+TGG + chr2.2:10166836-10166855 MS.gene043352:CDS 50.0%
!! GGAGGGGTGTTAAGGGAATT+TGG - chr2.2:10165774-10165793 None:intergenic 50.0%
!! GGTGGTGGTGGAATTGAAGT+TGG + chr2.2:10166848-10166867 MS.gene043352:CDS 50.0%
!! GGTTGCTGGTTCACCGAAAT+TGG + chr2.2:10166727-10166746 MS.gene043352:CDS 50.0%
GTCTGCTGAGGATTCAGCGA+CGG - chr2.2:10165835-10165854 None:intergenic 55.0%
TGAGGATTCAGCGACGGTGA+AGG - chr2.2:10165829-10165848 None:intergenic 55.0%
TGGAGGGATGATGAGGTTGG+AGG - chr2.2:10165754-10165773 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 10165471 10166949 10165471 ID=MS.gene043352
chr2.2 mRNA 10165471 10166949 10165471 ID=MS.gene043352.t1;Parent=MS.gene043352
chr2.2 exon 10165471 10166949 10165471 ID=MS.gene043352.t1.exon1;Parent=MS.gene043352.t1
chr2.2 CDS 10165471 10166949 10165471 ID=cds.MS.gene043352.t1;Parent=MS.gene043352.t1
Gene Sequence

>MS.gene043352

ATGAACACCACAAACACAAACACAAACACAAACATAACTCATGAGACCAAGTTCACCATGAAGGTGATAGAACATAGTCAAGTTGCACCACCACCAAATTCAGTTCCATCACCAACCATTCTCCCCTTAACTTTCTTCGACATACCATGGTTTTATTGTCACCCTGTTAAACGTATCTTCTTCTACCACTTTCCTCATCCAACTCATCACTTCCTTCAAACAACACTTCCCATTCTTAAACATTCACTTTCCTTAACCCTCCAACACTTCTTCCCTTTTTCCTCCAACCTCATCATCCCTCCAAATTCCCTTAACACCCCTCCTTATATACGTTATCTTGATGAAGACTCTCTATCCTTCACCGTCGCTGAATCCTCAGCAGACTTCAACATCCTCGTATCTGATTCACAAGATGCTCAAAACTGGCACCATCTTGTTCCAAATTTACCTCCACCTCGTACTGAACAAGACAACATTCGTGTCATCCCTATAATGGCTATTCAGGTCACTGTTCTTCCAAATTCTGGGTTGTCTATTTGCTTAAGTTACAACCATCTTGCTGCAGACGGAAAATCACTTCATCATTTCATGAAATTCTGGGCCTCTCTTTCAAAAGCCATAGCAACAAACAACAATAATTTGTTTCTTGAATACTCTCCCCCTCTTGATCTTCCTTCCCATGAAAGAGATAGAGTTAAAGATCTAAAGGGTCTTATACTTGTATACTTGCAAGAATTAGAAGATTCTGAATCAAATGACATGGAATTCGCGGGTTTGGTTCAAGATTCTTATGTTAACAAGATAAGAACAACTTTAGTATTGAGCTATGAACAAGTCCAGAAGTTGAAGAAATGGGTTACTGATAAATGTAAAGAGAGTTATAGAATACAACACTTGTCAACTTTTGTTGTAACAAGCTCCTTGATTTGGTTTTGTATGATCAAATCAGAGGAGGGTGATAAAAAAAGTGATCAAGGTGATTGTGTTGATGTTGATGATCATTGTTATTTTGTGTTTCTTGCAGATTGTCGTGATAGCGGTGAATTTTCATTACCAAAAACTTATTTTGGAAATTGTCTTGCATCTTATATTGTGGCTGTAAAGAGAGATGAGTTAATTGGAAAAAATGGGATTGTTGTGGCTTCAATTGGTATTGAAAAGAAGATAAGAGATTTTAAGAGTGATGCTTTGTTAGGAGCTGAAACATTGATGTCTGATTATAGGGAAATATCAAAACCTGGTAAGTCGGTTACTGTGGTTGCTGGTTCACCGAAATTGGCTGTTTATGAAACTGATTTTGGGTGGGGGAAGCCTAAGAAATCTGATGCAGTTCATCTTGATTCATCCGGTTCGATTTCTCTTTCTGATTGTAGAGATGGTGGTGGTGGAATTGAAGTTGGTTTGGCTCTTGAGAGGTCTAGAATGACCAATTTCATTAACATCTTTCAAGAACAACTTGATAATATTTGTAGCATGTGA
Protein sequence

>MS.gene043352.t1

MNTTNTNTNTNITHETKFTMKVIEHSQVAPPPNSVPSPTILPLTFFDIPWFYCHPVKRIFFYHFPHPTHHFLQTTLPILKHSLSLTLQHFFPFSSNLIIPPNSLNTPPYIRYLDEDSLSFTVAESSADFNILVSDSQDAQNWHHLVPNLPPPRTEQDNIRVIPIMAIQVTVLPNSGLSICLSYNHLAADGKSLHHFMKFWASLSKAIATNNNNLFLEYSPPLDLPSHERDRVKDLKGLILVYLQELEDSESNDMEFAGLVQDSYVNKIRTTLVLSYEQVQKLKKWVTDKCKESYRIQHLSTFVVTSSLIWFCMIKSEEGDKKSDQGDCVDVDDHCYFVFLADCRDSGEFSLPKTYFGNCLASYIVAVKRDELIGKNGIVVASIGIEKKIRDFKSDALLGAETLMSDYREISKPGKSVTVVAGSPKLAVYETDFGWGKPKKSDAVHLDSSGSISLSDCRDGGGGIEVGLALERSRMTNFINIFQEQLDNICSM