Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89985.t1 | XP_003606250.1 | 100 | 204 | 0 | 0 | 1 | 204 | 103 | 306 | 2.40E-114 | 421.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89985.t1 | G7JKY7 | 100.0 | 204 | 0 | 0 | 1 | 204 | 103 | 306 | 1.7e-114 | 421.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene89985 | 0.861398 | 1.05E-63 | -1.69E-46 |
| MS.gene04918 | MS.gene89985 | 0.827638 | 1.40E-54 | -1.69E-46 |
| MS.gene04920 | MS.gene89985 | 0.81204 | 5.18E-51 | -1.69E-46 |
| MS.gene049254 | MS.gene89985 | 0.848583 | 5.59E-60 | -1.69E-46 |
| MS.gene049283 | MS.gene89985 | 0.826022 | 3.41E-54 | -1.69E-46 |
| MS.gene049350 | MS.gene89985 | 0.861931 | 7.25E-64 | -1.69E-46 |
| MS.gene049368 | MS.gene89985 | 0.829639 | 4.60E-55 | -1.69E-46 |
| MS.gene049499 | MS.gene89985 | 0.839486 | 1.54E-57 | -1.69E-46 |
| MS.gene049711 | MS.gene89985 | 0.859551 | 3.83E-63 | -1.69E-46 |
| MS.gene049807 | MS.gene89985 | 0.840384 | 8.98E-58 | -1.69E-46 |
| MS.gene049821 | MS.gene89985 | 0.810362 | 1.20E-50 | -1.69E-46 |
| MS.gene049850 | MS.gene89985 | 0.823279 | 1.51E-53 | -1.69E-46 |
| MS.gene049983 | MS.gene89985 | 0.856418 | 3.26E-62 | -1.69E-46 |
| MS.gene050253 | MS.gene89985 | 0.803571 | 3.27E-49 | -1.69E-46 |
| MS.gene050273 | MS.gene89985 | 0.807062 | 6.07E-50 | -1.69E-46 |
| MS.gene050300 | MS.gene89985 | -0.810559 | 1.09E-50 | -1.69E-46 |
| MS.gene050414 | MS.gene89985 | 0.825901 | 3.64E-54 | -1.69E-46 |
| MS.gene050559 | MS.gene89985 | 0.800956 | 1.13E-48 | -1.69E-46 |
| MS.gene050646 | MS.gene89985 | 0.83011 | 3.53E-55 | -1.69E-46 |
| MS.gene050654 | MS.gene89985 | 0.820315 | 7.32E-53 | -1.69E-46 |
| MS.gene050901 | MS.gene89985 | 0.828618 | 8.13E-55 | -1.69E-46 |
| MS.gene051003 | MS.gene89985 | 0.802637 | 5.11E-49 | -1.69E-46 |
| MS.gene051198 | MS.gene89985 | 0.807127 | 5.88E-50 | -1.69E-46 |
| MS.gene051201 | MS.gene89985 | 0.814553 | 1.45E-51 | -1.69E-46 |
| MS.gene051234 | MS.gene89985 | 0.814824 | 1.27E-51 | -1.69E-46 |
| MS.gene051247 | MS.gene89985 | 0.829915 | 3.94E-55 | -1.69E-46 |
| MS.gene051279 | MS.gene89985 | 0.821277 | 4.40E-53 | -1.69E-46 |
| MS.gene051332 | MS.gene89985 | 0.830906 | 2.26E-55 | -1.69E-46 |
| MS.gene051343 | MS.gene89985 | 0.818237 | 2.18E-52 | -1.69E-46 |
| MS.gene051490 | MS.gene89985 | 0.814958 | 1.18E-51 | -1.69E-46 |
| MS.gene051628 | MS.gene89985 | 0.814035 | 1.89E-51 | -1.69E-46 |
| MS.gene051676 | MS.gene89985 | 0.814187 | 1.75E-51 | -1.69E-46 |
| MS.gene051700 | MS.gene89985 | 0.82762 | 1.41E-54 | -1.69E-46 |
| MS.gene051719 | MS.gene89985 | 0.841592 | 4.33E-58 | -1.69E-46 |
| MS.gene051720 | MS.gene89985 | 0.856424 | 3.25E-62 | -1.69E-46 |
| MS.gene051744 | MS.gene89985 | 0.830761 | 2.45E-55 | -1.69E-46 |
| MS.gene051800 | MS.gene89985 | 0.801935 | 7.12E-49 | -1.69E-46 |
| MS.gene051852 | MS.gene89985 | 0.822302 | 2.55E-53 | -1.69E-46 |
| MS.gene051902 | MS.gene89985 | 0.812673 | 3.77E-51 | -1.69E-46 |
| MS.gene051975 | MS.gene89985 | 0.815005 | 1.15E-51 | -1.69E-46 |
| MS.gene052016 | MS.gene89985 | 0.85657 | 2.94E-62 | -1.69E-46 |
| MS.gene052072 | MS.gene89985 | 0.822958 | 1.79E-53 | -1.69E-46 |
| MS.gene052168 | MS.gene89985 | 0.816936 | 4.28E-52 | -1.69E-46 |
| MS.gene052239 | MS.gene89985 | -0.810513 | 1.11E-50 | -1.69E-46 |
| MS.gene052307 | MS.gene89985 | 0.831657 | 1.47E-55 | -1.69E-46 |
| MS.gene052335 | MS.gene89985 | 0.800416 | 1.46E-48 | -1.69E-46 |
| MS.gene052463 | MS.gene89985 | 0.902401 | 1.01E-78 | -1.69E-46 |
| MS.gene052516 | MS.gene89985 | 0.844889 | 5.72E-59 | -1.69E-46 |
| MS.gene052519 | MS.gene89985 | 0.811607 | 6.43E-51 | -1.69E-46 |
| MS.gene052533 | MS.gene89985 | 0.839346 | 1.67E-57 | -1.69E-46 |
| MS.gene052534 | MS.gene89985 | 0.82267 | 2.09E-53 | -1.69E-46 |
| MS.gene052582 | MS.gene89985 | 0.820355 | 7.17E-53 | -1.69E-46 |
| MS.gene052584 | MS.gene89985 | 0.822146 | 2.77E-53 | -1.69E-46 |
| MS.gene052609 | MS.gene89985 | 0.819178 | 1.33E-52 | -1.69E-46 |
| MS.gene052673 | MS.gene89985 | -0.810944 | 8.96E-51 | -1.69E-46 |
| MS.gene052773 | MS.gene89985 | 0.809063 | 2.28E-50 | -1.69E-46 |
| MS.gene052775 | MS.gene89985 | 0.800997 | 1.11E-48 | -1.69E-46 |
| MS.gene053079 | MS.gene89985 | 0.830292 | 3.19E-55 | -1.69E-46 |
| MS.gene053143 | MS.gene89985 | 0.803014 | 4.27E-49 | -1.69E-46 |
| MS.gene053144 | MS.gene89985 | 0.800897 | 1.16E-48 | -1.69E-46 |
| MS.gene053226 | MS.gene89985 | 0.840079 | 1.08E-57 | -1.69E-46 |
| MS.gene053272 | MS.gene89985 | -0.807787 | 4.26E-50 | -1.69E-46 |
| MS.gene05350 | MS.gene89985 | 0.811015 | 8.65E-51 | -1.69E-46 |
| MS.gene053581 | MS.gene89985 | 0.832928 | 7.14E-56 | -1.69E-46 |
| MS.gene053599 | MS.gene89985 | 0.80053 | 1.38E-48 | -1.69E-46 |
| MS.gene05364 | MS.gene89985 | 0.810955 | 8.91E-51 | -1.69E-46 |
| MS.gene054142 | MS.gene89985 | 0.822882 | 1.87E-53 | -1.69E-46 |
| MS.gene054178 | MS.gene89985 | 0.842323 | 2.77E-58 | -1.69E-46 |
| MS.gene05422 | MS.gene89985 | 0.814994 | 1.16E-51 | -1.69E-46 |
| MS.gene05423 | MS.gene89985 | 0.858328 | 8.88E-63 | -1.69E-46 |
| MS.gene054479 | MS.gene89985 | 0.808883 | 2.49E-50 | -1.69E-46 |
| MS.gene054576 | MS.gene89985 | 0.81916 | 1.34E-52 | -1.69E-46 |
| MS.gene05458 | MS.gene89985 | 0.866763 | 2.25E-65 | -1.69E-46 |
| MS.gene054651 | MS.gene89985 | 0.859002 | 5.59E-63 | -1.69E-46 |
| MS.gene054709 | MS.gene89985 | 0.811033 | 8.57E-51 | -1.69E-46 |
| MS.gene054775 | MS.gene89985 | 0.83919 | 1.84E-57 | -1.69E-46 |
| MS.gene054892 | MS.gene89985 | 0.802514 | 5.41E-49 | -1.69E-46 |
| MS.gene054893 | MS.gene89985 | 0.805366 | 1.38E-49 | -1.69E-46 |
| MS.gene054991 | MS.gene89985 | 0.851651 | 7.72E-61 | -1.69E-46 |
| MS.gene055229 | MS.gene89985 | 0.805085 | 1.58E-49 | -1.69E-46 |
| MS.gene055310 | MS.gene89985 | 0.800204 | 1.61E-48 | -1.69E-46 |
| MS.gene055313 | MS.gene89985 | 0.822088 | 2.86E-53 | -1.69E-46 |
| MS.gene055386 | MS.gene89985 | 0.822968 | 1.78E-53 | -1.69E-46 |
| MS.gene05539 | MS.gene89985 | 0.813711 | 2.23E-51 | -1.69E-46 |
| MS.gene055465 | MS.gene89985 | 0.833654 | 4.71E-56 | -1.69E-46 |
| MS.gene055466 | MS.gene89985 | 0.823048 | 1.71E-53 | -1.69E-46 |
| MS.gene055468 | MS.gene89985 | 0.81925 | 1.28E-52 | -1.69E-46 |
| MS.gene055469 | MS.gene89985 | 0.827373 | 1.62E-54 | -1.69E-46 |
| MS.gene055513 | MS.gene89985 | 0.87647 | 1.36E-68 | -1.69E-46 |
| MS.gene05552 | MS.gene89985 | 0.84205 | 3.28E-58 | -1.69E-46 |
| MS.gene055580 | MS.gene89985 | -0.801887 | 7.28E-49 | -1.69E-46 |
| MS.gene055586 | MS.gene89985 | 0.801195 | 1.01E-48 | -1.69E-46 |
| MS.gene055724 | MS.gene89985 | 0.812893 | 3.37E-51 | -1.69E-46 |
| MS.gene055841 | MS.gene89985 | 0.801192 | 1.01E-48 | -1.69E-46 |
| MS.gene055864 | MS.gene89985 | 0.821838 | 3.26E-53 | -1.69E-46 |
| MS.gene055926 | MS.gene89985 | 0.828688 | 7.82E-55 | -1.69E-46 |
| MS.gene056045 | MS.gene89985 | 0.832932 | 7.13E-56 | -1.69E-46 |
| MS.gene05604 | MS.gene89985 | 0.805239 | 1.47E-49 | -1.69E-46 |
| MS.gene056082 | MS.gene89985 | 0.842745 | 2.14E-58 | -1.69E-46 |
| MS.gene056174 | MS.gene89985 | 0.828215 | 1.02E-54 | -1.69E-46 |
| MS.gene056181 | MS.gene89985 | 0.812624 | 3.86E-51 | -1.69E-46 |
| MS.gene056215 | MS.gene89985 | 0.825001 | 5.95E-54 | -1.69E-46 |
| MS.gene056308 | MS.gene89985 | 0.86366 | 2.12E-64 | -1.69E-46 |
| MS.gene056390 | MS.gene89985 | 0.831797 | 1.36E-55 | -1.69E-46 |
| MS.gene056509 | MS.gene89985 | 0.82494 | 6.15E-54 | -1.69E-46 |
| MS.gene056595 | MS.gene89985 | 0.81198 | 5.34E-51 | -1.69E-46 |
| MS.gene056677 | MS.gene89985 | 0.802542 | 5.34E-49 | -1.69E-46 |
| MS.gene056699 | MS.gene89985 | 0.856112 | 4.01E-62 | -1.69E-46 |
| MS.gene056776 | MS.gene89985 | 0.831785 | 1.37E-55 | -1.69E-46 |
| MS.gene056779 | MS.gene89985 | 0.80058 | 1.35E-48 | -1.69E-46 |
| MS.gene05686 | MS.gene89985 | 0.846592 | 1.97E-59 | -1.69E-46 |
| MS.gene05690 | MS.gene89985 | 0.82074 | 5.85E-53 | -1.69E-46 |
| MS.gene056973 | MS.gene89985 | 0.811441 | 6.99E-51 | -1.69E-46 |
| MS.gene057034 | MS.gene89985 | 0.836649 | 8.27E-57 | -1.69E-46 |
| MS.gene057102 | MS.gene89985 | 0.870711 | 1.19E-66 | -1.69E-46 |
| MS.gene057208 | MS.gene89985 | 0.833867 | 4.17E-56 | -1.69E-46 |
| MS.gene057294 | MS.gene89985 | 0.804931 | 1.70E-49 | -1.69E-46 |
| MS.gene057371 | MS.gene89985 | 0.811794 | 5.86E-51 | -1.69E-46 |
| MS.gene057611 | MS.gene89985 | 0.807125 | 5.89E-50 | -1.69E-46 |
| MS.gene057915 | MS.gene89985 | 0.813777 | 2.16E-51 | -1.69E-46 |
| MS.gene057919 | MS.gene89985 | 0.814003 | 1.92E-51 | -1.69E-46 |
| MS.gene057921 | MS.gene89985 | 0.816621 | 5.03E-52 | -1.69E-46 |
| MS.gene058159 | MS.gene89985 | 0.820084 | 8.27E-53 | -1.69E-46 |
| MS.gene058279 | MS.gene89985 | 0.830929 | 2.23E-55 | -1.69E-46 |
| MS.gene058289 | MS.gene89985 | 0.80151 | 8.70E-49 | -1.69E-46 |
| MS.gene058330 | MS.gene89985 | 0.809154 | 2.18E-50 | -1.69E-46 |
| MS.gene058339 | MS.gene89985 | 0.827306 | 1.68E-54 | -1.69E-46 |
| MS.gene05847 | MS.gene89985 | 0.805525 | 1.28E-49 | -1.69E-46 |
| MS.gene058492 | MS.gene89985 | 0.80967 | 1.69E-50 | -1.69E-46 |
| MS.gene058625 | MS.gene89985 | 0.818892 | 1.55E-52 | -1.69E-46 |
| MS.gene058668 | MS.gene89985 | 0.808163 | 3.54E-50 | -1.69E-46 |
| MS.gene058681 | MS.gene89985 | 0.811058 | 8.46E-51 | -1.69E-46 |
| MS.gene058781 | MS.gene89985 | 0.801155 | 1.03E-48 | -1.69E-46 |
| MS.gene058783 | MS.gene89985 | 0.801162 | 1.03E-48 | -1.69E-46 |
| MS.gene058852 | MS.gene89985 | 0.80261 | 5.17E-49 | -1.69E-46 |
| MS.gene05894 | MS.gene89985 | 0.802924 | 4.45E-49 | -1.69E-46 |
| MS.gene05897 | MS.gene89985 | 0.810882 | 9.24E-51 | -1.69E-46 |
| MS.gene059146 | MS.gene89985 | 0.802522 | 5.39E-49 | -1.69E-46 |
| MS.gene059170 | MS.gene89985 | 0.846498 | 2.09E-59 | -1.69E-46 |
| MS.gene059439 | MS.gene89985 | 0.820924 | 5.30E-53 | -1.69E-46 |
| MS.gene059570 | MS.gene89985 | 0.801249 | 9.84E-49 | -1.69E-46 |
| MS.gene059625 | MS.gene89985 | 0.817157 | 3.81E-52 | -1.69E-46 |
| MS.gene059778 | MS.gene89985 | 0.806489 | 8.02E-50 | -1.69E-46 |
| MS.gene05985 | MS.gene89985 | -0.851341 | 9.46E-61 | -1.69E-46 |
| MS.gene059965 | MS.gene89985 | 0.847202 | 1.34E-59 | -1.69E-46 |
| MS.gene060365 | MS.gene89985 | 0.83215 | 1.11E-55 | -1.69E-46 |
| MS.gene060373 | MS.gene89985 | 0.805484 | 1.31E-49 | -1.69E-46 |
| MS.gene060435 | MS.gene89985 | 0.801509 | 8.71E-49 | -1.69E-46 |
| MS.gene060519 | MS.gene89985 | 0.822154 | 2.76E-53 | -1.69E-46 |
| MS.gene060527 | MS.gene89985 | 0.801765 | 7.71E-49 | -1.69E-46 |
| MS.gene06052 | MS.gene89985 | 0.806005 | 1.02E-49 | -1.69E-46 |
| MS.gene060587 | MS.gene89985 | 0.802595 | 5.21E-49 | -1.69E-46 |
| MS.gene060777 | MS.gene89985 | 0.836304 | 1.01E-56 | -1.69E-46 |
| MS.gene060932 | MS.gene89985 | 0.835591 | 1.53E-56 | -1.69E-46 |
| MS.gene060933 | MS.gene89985 | 0.828752 | 7.55E-55 | -1.69E-46 |
| MS.gene060935 | MS.gene89985 | 0.832812 | 7.63E-56 | -1.69E-46 |
| MS.gene060937 | MS.gene89985 | 0.845565 | 3.75E-59 | -1.69E-46 |
| MS.gene061100 | MS.gene89985 | 0.876127 | 1.79E-68 | -1.69E-46 |
| MS.gene061160 | MS.gene89985 | 0.820008 | 8.60E-53 | -1.69E-46 |
| MS.gene061188 | MS.gene89985 | 0.840822 | 6.90E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89985.t1 | MTR_4g055230 | 100.000 | 204 | 0 | 0 | 1 | 204 | 103 | 306 | 2.35e-152 | 424 |
| MS.gene89985.t1 | MTR_2g084965 | 73.399 | 203 | 48 | 3 | 4 | 204 | 111 | 309 | 1.04e-100 | 294 |
| MS.gene89985.t1 | MTR_7g103900 | 57.075 | 212 | 75 | 6 | 2 | 203 | 104 | 309 | 7.63e-75 | 228 |
| MS.gene89985.t1 | MTR_7g114790 | 40.367 | 218 | 101 | 6 | 8 | 203 | 95 | 305 | 1.09e-44 | 150 |
| MS.gene89985.t1 | MTR_7g095100 | 38.739 | 222 | 109 | 7 | 2 | 203 | 92 | 306 | 1.91e-42 | 145 |
| MS.gene89985.t1 | MTR_1g081260 | 38.967 | 213 | 105 | 7 | 8 | 203 | 98 | 302 | 1.21e-41 | 142 |
| MS.gene89985.t1 | MTR_7g103900 | 47.727 | 132 | 57 | 4 | 2 | 127 | 104 | 229 | 2.64e-29 | 108 |
| MS.gene89985.t1 | MTR_4g104760 | 33.533 | 167 | 103 | 4 | 9 | 169 | 102 | 266 | 2.78e-29 | 110 |
| MS.gene89985.t1 | MTR_5g069570 | 36.095 | 169 | 102 | 3 | 2 | 169 | 98 | 261 | 6.04e-29 | 110 |
| MS.gene89985.t1 | MTR_3g106250 | 34.940 | 166 | 87 | 6 | 11 | 169 | 109 | 260 | 1.20e-28 | 109 |
| MS.gene89985.t1 | MTR_3g076610 | 34.078 | 179 | 96 | 5 | 9 | 169 | 100 | 274 | 1.86e-28 | 109 |
| MS.gene89985.t1 | MTR_5g096570 | 33.728 | 169 | 105 | 3 | 2 | 169 | 1254 | 1416 | 2.15e-27 | 109 |
| MS.gene89985.t1 | MTR_3g082430 | 35.882 | 170 | 101 | 5 | 2 | 169 | 81 | 244 | 4.32e-27 | 104 |
| MS.gene89985.t1 | MTR_1g013100 | 32.584 | 178 | 94 | 8 | 11 | 169 | 90 | 260 | 2.95e-21 | 89.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89985.t1 | AT2G27770 | 59.024 | 205 | 75 | 6 | 8 | 203 | 116 | 320 | 1.05e-78 | 238 |
| MS.gene89985.t1 | AT5G28150 | 40.404 | 198 | 108 | 4 | 9 | 203 | 98 | 288 | 2.91e-43 | 146 |
| MS.gene89985.t1 | AT3G04860 | 39.086 | 197 | 110 | 4 | 9 | 202 | 98 | 287 | 2.14e-42 | 144 |
| MS.gene89985.t1 | AT2G36470 | 38.793 | 232 | 96 | 6 | 11 | 202 | 101 | 326 | 1.49e-38 | 135 |
| MS.gene89985.t1 | AT5G11000 | 34.804 | 204 | 114 | 5 | 8 | 202 | 112 | 305 | 7.41e-35 | 127 |
| MS.gene89985.t1 | AT4G12690 | 31.905 | 210 | 120 | 5 | 2 | 202 | 88 | 283 | 1.29e-32 | 119 |
| MS.gene89985.t1 | AT4G12690 | 31.905 | 210 | 120 | 5 | 2 | 202 | 88 | 283 | 1.29e-32 | 119 |
| MS.gene89985.t1 | AT2G04220 | 33.146 | 178 | 112 | 3 | 2 | 178 | 92 | 263 | 2.41e-31 | 116 |
| MS.gene89985.t1 | AT3G13229 | 32.778 | 180 | 111 | 5 | 2 | 177 | 72 | 245 | 5.65e-29 | 109 |
| MS.gene89985.t1 | AT5G48270 | 33.526 | 173 | 103 | 5 | 2 | 169 | 104 | 269 | 1.80e-23 | 95.9 |
| MS.gene89985.t1 | AT2G25200 | 34.459 | 148 | 81 | 5 | 9 | 156 | 126 | 257 | 9.34e-18 | 80.5 |
Find 22 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTTCAATCCTCAAGCTTC+TGG | 0.251073 | 4.2:+48122358 | MS.gene89985:CDS |
| GTTTATTGGTTGATTTGCTT+TGG | 0.298116 | 4.2:+48122320 | MS.gene89985:CDS |
| ATTGTTGTTGATTCAGAAAT+CGG | 0.347236 | 4.2:+48121995 | MS.gene89985:CDS |
| TGCTTTGGGATGTTCATGAT+TGG | 0.357421 | 4.2:+48122335 | MS.gene89985:CDS |
| TTTATTGGTTGATTTGCTTT+GGG | 0.368472 | 4.2:+48122321 | MS.gene89985:CDS |
| GTTTAGAACAAGAAGTGGTT+TGG | 0.386790 | 4.2:+48122396 | MS.gene89985:CDS |
| ATCTCGAAGAGAACATTGTT+CGG | 0.407078 | 4.2:+48122093 | MS.gene89985:CDS |
| TCAAATGCTAAATATGAAAC+AGG | 0.418090 | 4.2:+48121941 | MS.gene89985:CDS |
| TCAATATTTACGCTTAAAAC+CGG | 0.421635 | 4.2:-48122226 | None:intergenic |
| TCTCGAAGAGAACATTGTTC+GGG | 0.437578 | 4.2:+48122094 | MS.gene89985:CDS |
| TATGCATGTAAGAGCTCATA+AGG | 0.446585 | 4.2:+48122490 | MS.gene89985:CDS |
| AATGAAAGATAGTGTTCCTA+TGG | 0.472093 | 4.2:+48122054 | MS.gene89985:CDS |
| GTTTAATAATAAATCTTCAC+CGG | 0.501382 | 4.2:+48122207 | MS.gene89985:CDS |
| TCAGAAATCGGTTTAATCCT+CGG | 0.502608 | 4.2:+48122007 | MS.gene89985:CDS |
| AAATGCAGCGTCGAAAATGA+AGG | 0.507502 | 4.2:+48122184 | MS.gene89985:CDS |
| TTCATGTTTAGAACAAGAAG+TGG | 0.540769 | 4.2:+48122391 | MS.gene89985:CDS |
| AGAATGTGATTCGTGTGAAG+AGG | 0.623101 | 4.2:+48122254 | MS.gene89985:CDS |
| GTGGTTTGGATAGTAGATTG+TGG | 0.623521 | 4.2:+48122410 | MS.gene89985:CDS |
| TTCGTGTGAAGAGGTTGCAA+TGG | 0.634580 | 4.2:+48122263 | MS.gene89985:CDS |
| ACAGCATAACCAGAAGCTTG+AGG | 0.635672 | 4.2:-48122367 | None:intergenic |
| AATGCAGCGTCGAAAATGAA+GGG | 0.637286 | 4.2:+48122185 | MS.gene89985:CDS |
| TAACAGTTTCGGAATCTCCG+AGG | 0.682405 | 4.2:-48122024 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTTAATAATAAATCTTCAC+CGG | + | chr4.2:48122207-48122226 | MS.gene89985:CDS | 20.0% |
| !!! | CATTTTTTTAGTAACAGTTT+CGG | - | chr4.2:48122038-48122057 | None:intergenic | 20.0% |
| ! | CAATAAACCATCAACAAAAA+TGG | - | chr4.2:48122308-48122327 | None:intergenic | 25.0% |
| ! | TCAAATGCTAAATATGAAAC+AGG | + | chr4.2:48121941-48121960 | MS.gene89985:CDS | 25.0% |
| ! | TCAATATTTACGCTTAAAAC+CGG | - | chr4.2:48122229-48122248 | None:intergenic | 25.0% |
| !!! | AATCAAACCATTTTTGTTGA+TGG | + | chr4.2:48122298-48122317 | MS.gene89985:CDS | 25.0% |
| !!! | ATTGTTGTTGATTCAGAAAT+CGG | + | chr4.2:48121995-48122014 | MS.gene89985:CDS | 25.0% |
| !!! | ATTTTAAGGTAACTGTTTTG+TGG | + | chr4.2:48121912-48121931 | MS.gene89985:CDS | 25.0% |
| !!! | CATTTTTGTTGATGGTTTAT+TGG | + | chr4.2:48122306-48122325 | MS.gene89985:CDS | 25.0% |
| !!! | GCTAGATTTTGAAGATTTTA+AGG | + | chr4.2:48121898-48121917 | MS.gene89985:CDS | 25.0% |
| !!! | TTTATTGGTTGATTTGCTTT+GGG | + | chr4.2:48122321-48122340 | MS.gene89985:CDS | 25.0% |
| !!! | TTTTAAGGTAACTGTTTTGT+GGG | + | chr4.2:48121913-48121932 | MS.gene89985:CDS | 25.0% |
| AATGAAAGATAGTGTTCCTA+TGG | + | chr4.2:48122054-48122073 | MS.gene89985:CDS | 30.0% | |
| ! | AAAGCTCAATTTTGTGATAG+TGG | + | chr4.2:48122139-48122158 | MS.gene89985:CDS | 30.0% |
| ! | TTCATGTTTAGAACAAGAAG+TGG | + | chr4.2:48122391-48122410 | MS.gene89985:CDS | 30.0% |
| !! | ACTACATAAAAAGCATCAAC+AGG | - | chr4.2:48121974-48121993 | None:intergenic | 30.0% |
| !! | GTTTATTGGTTGATTTGCTT+TGG | + | chr4.2:48122320-48122339 | MS.gene89985:CDS | 30.0% |
| ATCTCGAAGAGAACATTGTT+CGG | + | chr4.2:48122093-48122112 | MS.gene89985:CDS | 35.0% | |
| ! | AATAGCGAAATTTTCGCCAT+AGG | - | chr4.2:48122073-48122092 | None:intergenic | 35.0% |
| ! | AGGTTGCAATGGAATTTTAG+AGG | + | chr4.2:48122274-48122293 | MS.gene89985:CDS | 35.0% |
| ! | GGTTGCAATGGAATTTTAGA+GGG | + | chr4.2:48122275-48122294 | MS.gene89985:CDS | 35.0% |
| ! | GTTTAGAACAAGAAGTGGTT+TGG | + | chr4.2:48122396-48122415 | MS.gene89985:CDS | 35.0% |
| ! | TCAGAAATCGGTTTAATCCT+CGG | + | chr4.2:48122007-48122026 | MS.gene89985:CDS | 35.0% |
| !! | TAAAAAGCATCAACAGGTTC+TGG | - | chr4.2:48121968-48121987 | None:intergenic | 35.0% |
| AGAATGTGATTCGTGTGAAG+AGG | + | chr4.2:48122254-48122273 | MS.gene89985:CDS | 40.0% | |
| TCTCGAAGAGAACATTGTTC+GGG | + | chr4.2:48122094-48122113 | MS.gene89985:CDS | 40.0% | |
| TTCTTCAATCCTCAAGCTTC+TGG | + | chr4.2:48122358-48122377 | MS.gene89985:CDS | 40.0% | |
| ! | AAATGCAGCGTCGAAAATGA+AGG | + | chr4.2:48122184-48122203 | MS.gene89985:CDS | 40.0% |
| ! | AATGCAGCGTCGAAAATGAA+GGG | + | chr4.2:48122185-48122204 | MS.gene89985:CDS | 40.0% |
| ! | GTGGTTTGGATAGTAGATTG+TGG | + | chr4.2:48122410-48122429 | MS.gene89985:CDS | 40.0% |
| !! | TGCTTTGGGATGTTCATGAT+TGG | + | chr4.2:48122335-48122354 | MS.gene89985:CDS | 40.0% |
| ACAGCATAACCAGAAGCTTG+AGG | - | chr4.2:48122370-48122389 | None:intergenic | 45.0% | |
| TAACAGTTTCGGAATCTCCG+AGG | - | chr4.2:48122027-48122046 | None:intergenic | 45.0% | |
| TTCGTGTGAAGAGGTTGCAA+TGG | + | chr4.2:48122263-48122282 | MS.gene89985:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 48121896 | 48122510 | 48121896 | ID=MS.gene89985 |
| chr4.2 | mRNA | 48121896 | 48122510 | 48121896 | ID=MS.gene89985.t1;Parent=MS.gene89985 |
| chr4.2 | exon | 48121896 | 48122510 | 48121896 | ID=MS.gene89985.t1.exon1;Parent=MS.gene89985.t1 |
| chr4.2 | CDS | 48121896 | 48122510 | 48121896 | ID=cds.MS.gene89985.t1;Parent=MS.gene89985.t1 |
| Gene Sequence |
| Protein sequence |
