Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87922.t1 | XP_013451545.1 | 97.9 | 187 | 4 | 0 | 1 | 187 | 1 | 187 | 1.50E-72 | 283.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87922.t1 | Q9SE95 | 69.2 | 169 | 49 | 1 | 6 | 174 | 5 | 170 | 1.5e-44 | 181.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87922.t1 | A0A072U8Z3 | 97.9 | 187 | 4 | 0 | 1 | 187 | 1 | 187 | 1.1e-72 | 283.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene87922 | 0.849817 | 2.53E-60 | -1.69E-46 |
| MS.gene04918 | MS.gene87922 | 0.807721 | 4.40E-50 | -1.69E-46 |
| MS.gene049254 | MS.gene87922 | 0.804746 | 1.86E-49 | -1.69E-46 |
| MS.gene049283 | MS.gene87922 | 0.80426 | 2.35E-49 | -1.69E-46 |
| MS.gene049350 | MS.gene87922 | 0.846054 | 2.76E-59 | -1.69E-46 |
| MS.gene049368 | MS.gene87922 | 0.853593 | 2.16E-61 | -1.69E-46 |
| MS.gene049499 | MS.gene87922 | 0.803864 | 2.84E-49 | -1.69E-46 |
| MS.gene049711 | MS.gene87922 | 0.814087 | 1.84E-51 | -1.69E-46 |
| MS.gene049807 | MS.gene87922 | 0.815265 | 1.01E-51 | -1.69E-46 |
| MS.gene049983 | MS.gene87922 | 0.826766 | 2.27E-54 | -1.69E-46 |
| MS.gene050300 | MS.gene87922 | -0.818555 | 1.84E-52 | -1.69E-46 |
| MS.gene050551 | MS.gene87922 | 0.802264 | 6.09E-49 | -1.69E-46 |
| MS.gene050646 | MS.gene87922 | 0.840345 | 9.20E-58 | -1.69E-46 |
| MS.gene050654 | MS.gene87922 | 0.802462 | 5.55E-49 | -1.69E-46 |
| MS.gene050689 | MS.gene87922 | 0.816843 | 4.49E-52 | -1.69E-46 |
| MS.gene050752 | MS.gene87922 | 0.800833 | 1.20E-48 | -1.69E-46 |
| MS.gene050838 | MS.gene87922 | 0.822087 | 2.86E-53 | -1.69E-46 |
| MS.gene051003 | MS.gene87922 | 0.848757 | 5.00E-60 | -1.69E-46 |
| MS.gene051234 | MS.gene87922 | 0.837204 | 5.97E-57 | -1.69E-46 |
| MS.gene051247 | MS.gene87922 | 0.80787 | 4.09E-50 | -1.69E-46 |
| MS.gene051343 | MS.gene87922 | 0.804308 | 2.30E-49 | -1.69E-46 |
| MS.gene051700 | MS.gene87922 | 0.823865 | 1.10E-53 | -1.69E-46 |
| MS.gene051744 | MS.gene87922 | 0.800085 | 1.70E-48 | -1.69E-46 |
| MS.gene05188 | MS.gene87922 | 0.818099 | 2.34E-52 | -1.69E-46 |
| MS.gene051902 | MS.gene87922 | 0.814759 | 1.31E-51 | -1.69E-46 |
| MS.gene051975 | MS.gene87922 | 0.831535 | 1.58E-55 | -1.69E-46 |
| MS.gene052072 | MS.gene87922 | 0.836905 | 7.11E-57 | -1.69E-46 |
| MS.gene052307 | MS.gene87922 | 0.812165 | 4.86E-51 | -1.69E-46 |
| MS.gene052434 | MS.gene87922 | 0.809383 | 1.94E-50 | -1.69E-46 |
| MS.gene052463 | MS.gene87922 | 0.853898 | 1.76E-61 | -1.69E-46 |
| MS.gene052516 | MS.gene87922 | 0.815435 | 9.26E-52 | -1.69E-46 |
| MS.gene052518 | MS.gene87922 | 0.823763 | 1.16E-53 | -1.69E-46 |
| MS.gene052519 | MS.gene87922 | 0.803689 | 3.09E-49 | -1.69E-46 |
| MS.gene052533 | MS.gene87922 | 0.805089 | 1.58E-49 | -1.69E-46 |
| MS.gene052609 | MS.gene87922 | 0.801481 | 8.82E-49 | -1.69E-46 |
| MS.gene052889 | MS.gene87922 | 0.803978 | 2.69E-49 | -1.69E-46 |
| MS.gene053058 | MS.gene87922 | 0.802755 | 4.83E-49 | -1.69E-46 |
| MS.gene053079 | MS.gene87922 | 0.820969 | 5.18E-53 | -1.69E-46 |
| MS.gene053122 | MS.gene87922 | 0.806672 | 7.34E-50 | -1.69E-46 |
| MS.gene053226 | MS.gene87922 | 0.843806 | 1.12E-58 | -1.69E-46 |
| MS.gene05364 | MS.gene87922 | 0.81841 | 1.99E-52 | -1.69E-46 |
| MS.gene054142 | MS.gene87922 | 0.804722 | 1.89E-49 | -1.69E-46 |
| MS.gene054403 | MS.gene87922 | 0.801423 | 9.07E-49 | -1.69E-46 |
| MS.gene05458 | MS.gene87922 | 0.851942 | 6.39E-61 | -1.69E-46 |
| MS.gene054651 | MS.gene87922 | 0.816809 | 4.57E-52 | -1.69E-46 |
| MS.gene05517 | MS.gene87922 | 0.81836 | 2.04E-52 | -1.69E-46 |
| MS.gene055468 | MS.gene87922 | 0.808471 | 3.05E-50 | -1.69E-46 |
| MS.gene055513 | MS.gene87922 | 0.865514 | 5.59E-65 | -1.69E-46 |
| MS.gene055580 | MS.gene87922 | -0.813948 | 1.98E-51 | -1.69E-46 |
| MS.gene056043 | MS.gene87922 | -0.821558 | 3.79E-53 | -1.69E-46 |
| MS.gene056181 | MS.gene87922 | 0.829108 | 6.19E-55 | -1.69E-46 |
| MS.gene056308 | MS.gene87922 | 0.838825 | 2.29E-57 | -1.69E-46 |
| MS.gene056390 | MS.gene87922 | 0.810039 | 1.41E-50 | -1.69E-46 |
| MS.gene056595 | MS.gene87922 | 0.800551 | 1.37E-48 | -1.69E-46 |
| MS.gene056699 | MS.gene87922 | 0.810092 | 1.37E-50 | -1.69E-46 |
| MS.gene056786 | MS.gene87922 | 0.800309 | 1.53E-48 | -1.69E-46 |
| MS.gene05686 | MS.gene87922 | 0.822753 | 2.00E-53 | -1.69E-46 |
| MS.gene056986 | MS.gene87922 | 0.808705 | 2.72E-50 | -1.69E-46 |
| MS.gene057034 | MS.gene87922 | 0.813297 | 2.75E-51 | -1.69E-46 |
| MS.gene057102 | MS.gene87922 | 0.8549 | 9.04E-62 | -1.69E-46 |
| MS.gene057208 | MS.gene87922 | 0.819468 | 1.14E-52 | -1.69E-46 |
| MS.gene05781 | MS.gene87922 | 0.807186 | 5.72E-50 | -1.69E-46 |
| MS.gene058149 | MS.gene87922 | 0.800999 | 1.11E-48 | -1.69E-46 |
| MS.gene058279 | MS.gene87922 | 0.801297 | 9.62E-49 | -1.69E-46 |
| MS.gene058625 | MS.gene87922 | 0.801653 | 8.13E-49 | -1.69E-46 |
| MS.gene058783 | MS.gene87922 | 0.813323 | 2.71E-51 | -1.69E-46 |
| MS.gene059146 | MS.gene87922 | 0.833199 | 6.12E-56 | -1.69E-46 |
| MS.gene059170 | MS.gene87922 | 0.811813 | 5.80E-51 | -1.69E-46 |
| MS.gene059227 | MS.gene87922 | 0.808746 | 2.66E-50 | -1.69E-46 |
| MS.gene059778 | MS.gene87922 | 0.805627 | 1.22E-49 | -1.69E-46 |
| MS.gene059842 | MS.gene87922 | 0.817423 | 3.32E-52 | -1.69E-46 |
| MS.gene05985 | MS.gene87922 | -0.829318 | 5.50E-55 | -1.69E-46 |
| MS.gene059901 | MS.gene87922 | -0.807881 | 4.07E-50 | -1.69E-46 |
| MS.gene059965 | MS.gene87922 | 0.800913 | 1.15E-48 | -1.69E-46 |
| MS.gene06011 | MS.gene87922 | 0.804075 | 2.57E-49 | -1.69E-46 |
| MS.gene060365 | MS.gene87922 | 0.809418 | 1.91E-50 | -1.69E-46 |
| MS.gene060429 | MS.gene87922 | 0.814239 | 1.70E-51 | -1.69E-46 |
| MS.gene060763 | MS.gene87922 | 0.858944 | 5.82E-63 | -1.69E-46 |
| MS.gene060933 | MS.gene87922 | 0.815495 | 8.98E-52 | -1.69E-46 |
| MS.gene060935 | MS.gene87922 | 0.838247 | 3.22E-57 | -1.69E-46 |
| MS.gene060937 | MS.gene87922 | 0.843899 | 1.05E-58 | -1.69E-46 |
| MS.gene061100 | MS.gene87922 | 0.84358 | 1.28E-58 | -1.69E-46 |
| MS.gene061188 | MS.gene87922 | 0.826014 | 3.42E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene023557 | MS.gene87922 | PPI |
| MS.gene87922 | MS.gene53384 | PPI |
| MS.gene87922 | MS.gene09068 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87922.t1 | MTR_6g027790 | 99.007 | 302 | 3 | 0 | 1 | 302 | 1 | 302 | 0.0 | 612 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87922.t1 | AT5G55000 | 81.145 | 297 | 53 | 1 | 6 | 302 | 5 | 298 | 1.77e-178 | 494 |
| MS.gene87922.t1 | AT5G55000 | 82.437 | 279 | 46 | 1 | 6 | 284 | 5 | 280 | 2.35e-170 | 474 |
Find 66 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCGAGGGGTTAATCTTTC+TGG | 0.140143 | 6.1:-10377547 | MS.gene87922:CDS |
| AAATCTTCGTGGTGCTTTAT+TGG | 0.215956 | 6.1:-10376392 | MS.gene87922:CDS |
| CTTCTTCCTTCCTCTTATTA+AGG | 0.218270 | 6.1:+10377635 | None:intergenic |
| TCTTCAATGTGCAAAGTTTC+GGG | 0.233983 | 6.1:-10376956 | MS.gene87922:intron |
| ATCTTCAATGTGCAAAGTTT+CGG | 0.272003 | 6.1:-10376957 | MS.gene87922:CDS |
| TTAGCGATTGTGTACGTTAT+TGG | 0.280603 | 6.1:+10380348 | None:intergenic |
| TAGCGATTGTGTACGTTATT+GGG | 0.299151 | 6.1:+10380349 | None:intergenic |
| TCGCAGGAGGGAAGAAATTC+TGG | 0.301822 | 6.1:-10380456 | MS.gene87922:intron |
| CTTCGTGGTGCTTTATTGGC+TGG | 0.321965 | 6.1:-10376388 | MS.gene87922:CDS |
| TGGGATCTTGGGAGAGAGTA+TGG | 0.324242 | 6.1:+10380368 | None:intergenic |
| GACACATTCTCAATTGGTTA+AGG | 0.327644 | 6.1:-10378390 | MS.gene87922:CDS |
| TAGGGAAGATGTGAATTTAC+TGG | 0.329065 | 6.1:-10376418 | MS.gene87922:intron |
| TCTGGAAGGAGCAAATTTAA+AGG | 0.330460 | 6.1:-10374262 | MS.gene87922:intron |
| CTGAATATTCAGAGCTGTTA+AGG | 0.330521 | 6.1:-10378339 | MS.gene87922:CDS |
| TGTGTACGTTATTGGGATCT+TGG | 0.339243 | 6.1:+10380356 | None:intergenic |
| TCCTCTTATTAAGGACATCT+TGG | 0.341120 | 6.1:+10377644 | None:intergenic |
| TCTGATTGCTCCGAGCAAAT+TGG | 0.346394 | 6.1:+10372442 | None:intergenic |
| CAATCTGGAGGGAGCTAATC+TGG | 0.361067 | 6.1:-10374280 | MS.gene87922:CDS |
| GTGTACGTTATTGGGATCTT+GGG | 0.373178 | 6.1:+10380357 | None:intergenic |
| AAATGTTAGGGGCTTATAGA+TGG | 0.404279 | 6.1:-10377670 | MS.gene87922:intron |
| ACACATTCTCAATTGGTTAA+GGG | 0.428609 | 6.1:-10378389 | MS.gene87922:CDS |
| TGGCATCGACCTCTCTAAAC+TGG | 0.430625 | 6.1:-10377527 | MS.gene87922:intron |
| ACTTCAGACACATTCTCAAT+TGG | 0.430939 | 6.1:-10378396 | MS.gene87922:CDS |
| ACGAAGCGCGGAAGATAAAT+CGG | 0.452142 | 6.1:+10380584 | None:intergenic |
| CAGATTAGCTCCCTCCAGAT+TGG | 0.453075 | 6.1:+10374281 | None:intergenic |
| TTCGTGGTGCTTTATTGGCT+GGG | 0.455181 | 6.1:-10376387 | MS.gene87922:CDS |
| TTGGAAGGAGCAAAGCTTGA+TGG | 0.460846 | 6.1:-10372467 | MS.gene87922:CDS |
| GTATACAATCTGAGAGAGTC+AGG | 0.465422 | 6.1:-10377570 | MS.gene87922:CDS |
| TGATCTTACTAATGCCAATC+TGG | 0.476212 | 6.1:-10374295 | MS.gene87922:CDS |
| TGAATATTCAGAGCTGTTAA+GGG | 0.481904 | 6.1:-10378338 | MS.gene87922:CDS |
| GACGCAGATAAGCTCGTTGA+AGG | 0.487337 | 6.1:+10373327 | None:intergenic |
| CCTGCGATCTGCACATCTAC+AGG | 0.491495 | 6.1:-10375827 | MS.gene87922:intron |
| ATAGCAGCGAGCATTGAATC+AGG | 0.492744 | 6.1:+10380403 | None:intergenic |
| TCCAAGATGTCCTTAATAAG+AGG | 0.492750 | 6.1:-10377645 | MS.gene87922:CDS |
| CAAAGTTGCATTATGAAAGA+TGG | 0.497185 | 6.1:+10376518 | None:intergenic |
| TTAGTAGGATGTAGCTTCTG+TGG | 0.501874 | 6.1:-10375856 | MS.gene87922:CDS |
| TCTGAGAGAGTCAGGTTTCG+AGG | 0.507145 | 6.1:-10377562 | MS.gene87922:CDS |
| AGGGAAGATGTGAATTTACT+GGG | 0.512755 | 6.1:-10376417 | MS.gene87922:intron |
| CTTGATGGTGCCAATTTGCT+CGG | 0.516763 | 6.1:-10372452 | MS.gene87922:CDS |
| CTTCAAAGCGCAAATCTACA+AGG | 0.519894 | 6.1:-10376358 | MS.gene87922:intron |
| CTGAGAGAGTCAGGTTTCGA+GGG | 0.520649 | 6.1:-10377561 | MS.gene87922:CDS |
| CACAAGTAGCTGATAGTACT+CGG | 0.521027 | 6.1:+10378312 | None:intergenic |
| TTTATCAATCAGGACTTGGA+AGG | 0.525684 | 6.1:-10372482 | MS.gene87922:intron |
| TTATAGGATGTTGATGCTGA+GGG | 0.533321 | 6.1:-10376543 | MS.gene87922:intron |
| TGGCGTGGTGCCCACTCTCG+AGG | 0.534975 | 6.1:-10378365 | MS.gene87922:CDS |
| AAGGTTAGCATTCTTCAACT+TGG | 0.536677 | 6.1:+10373346 | None:intergenic |
| TTTACTGGGGCAAATCTTCG+TGG | 0.542197 | 6.1:-10376403 | MS.gene87922:CDS |
| CCTGTAGATGTGCAGATCGC+AGG | 0.553123 | 6.1:+10375827 | None:intergenic |
| TAATAAGAGGAAGGAAGAAG+AGG | 0.574612 | 6.1:-10377632 | MS.gene87922:CDS |
| TCCGCGCTTCGTCTCAACAT+CGG | 0.582992 | 6.1:-10380573 | MS.gene87922:intron |
| GCGCGGAAGATAAATCGGAT+TGG | 0.584182 | 6.1:+10380589 | None:intergenic |
| TTTATAGGATGTTGATGCTG+AGG | 0.585562 | 6.1:-10376544 | MS.gene87922:intron |
| ATTCTCAATTGGTTAAGGGA+TGG | 0.600914 | 6.1:-10378385 | MS.gene87922:CDS |
| ATATTCAGCTTCCTCGAGAG+TGG | 0.601502 | 6.1:+10378354 | None:intergenic |
| AGATGTCCTTAATAAGAGGA+AGG | 0.603876 | 6.1:-10377641 | MS.gene87922:CDS |
| AAGAATTTACCAGTTTAGAG+AGG | 0.607566 | 6.1:+10377518 | None:intergenic |
| ACCGATGTTGAGACGAAGCG+CGG | 0.616509 | 6.1:+10380572 | None:intergenic |
| CTTACTAATGCCAATCTGGA+GGG | 0.617585 | 6.1:-10374291 | MS.gene87922:CDS |
| TATTCAGCTTCCTCGAGAGT+GGG | 0.618214 | 6.1:+10378355 | None:intergenic |
| CTGGAGGGAGCTAATCTGGA+AGG | 0.618931 | 6.1:-10374276 | MS.gene87922:CDS |
| ATGCTCGCTGCTATGTTCAG+TGG | 0.625132 | 6.1:-10380394 | MS.gene87922:CDS |
| CAATTGGTTAAGGGATGGCG+TGG | 0.626299 | 6.1:-10378380 | MS.gene87922:CDS |
| TCTTACTAATGCCAATCTGG+AGG | 0.628999 | 6.1:-10374292 | MS.gene87922:CDS |
| CAGTACGTGTCAATTCTGTA+CGG | 0.635724 | 6.1:+10377605 | None:intergenic |
| TGAGAGAGTCAGGTTTCGAG+GGG | 0.661863 | 6.1:-10377560 | MS.gene87922:CDS |
| GGGAAGATGTGAATTTACTG+GGG | 0.736367 | 6.1:-10376416 | MS.gene87922:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 10372439 | 10380622 | 10372439 | ID=MS.gene87922 |
| chr6.1 | mRNA | 10372439 | 10380622 | 10372439 | ID=MS.gene87922.t1;Parent=MS.gene87922 |
| chr6.1 | exon | 10380574 | 10380622 | 10380574 | ID=MS.gene87922.t1.exon1;Parent=MS.gene87922.t1 |
| chr6.1 | CDS | 10380574 | 10380622 | 10380574 | ID=cds.MS.gene87922.t1;Parent=MS.gene87922.t1 |
| chr6.1 | exon | 10380363 | 10380472 | 10380363 | ID=MS.gene87922.t1.exon2;Parent=MS.gene87922.t1 |
| chr6.1 | CDS | 10380363 | 10380472 | 10380363 | ID=cds.MS.gene87922.t1;Parent=MS.gene87922.t1 |
| chr6.1 | exon | 10378315 | 10378449 | 10378315 | ID=MS.gene87922.t1.exon3;Parent=MS.gene87922.t1 |
| chr6.1 | CDS | 10378315 | 10378449 | 10378315 | ID=cds.MS.gene87922.t1;Parent=MS.gene87922.t1 |
| chr6.1 | exon | 10377528 | 10377683 | 10377528 | ID=MS.gene87922.t1.exon4;Parent=MS.gene87922.t1 |
| chr6.1 | CDS | 10377528 | 10377683 | 10377528 | ID=cds.MS.gene87922.t1;Parent=MS.gene87922.t1 |
| chr6.1 | exon | 10376957 | 10377040 | 10376957 | ID=MS.gene87922.t1.exon5;Parent=MS.gene87922.t1 |
| chr6.1 | CDS | 10376957 | 10377040 | 10376957 | ID=cds.MS.gene87922.t1;Parent=MS.gene87922.t1 |
| chr6.1 | exon | 10376513 | 10376559 | 10376513 | ID=MS.gene87922.t1.exon6;Parent=MS.gene87922.t1 |
| chr6.1 | CDS | 10376513 | 10376559 | 10376513 | ID=cds.MS.gene87922.t1;Parent=MS.gene87922.t1 |
| chr6.1 | exon | 10376359 | 10376432 | 10376359 | ID=MS.gene87922.t1.exon7;Parent=MS.gene87922.t1 |
| chr6.1 | CDS | 10376359 | 10376432 | 10376359 | ID=cds.MS.gene87922.t1;Parent=MS.gene87922.t1 |
| chr6.1 | exon | 10375828 | 10375886 | 10375828 | ID=MS.gene87922.t1.exon8;Parent=MS.gene87922.t1 |
| chr6.1 | CDS | 10375828 | 10375886 | 10375828 | ID=cds.MS.gene87922.t1;Parent=MS.gene87922.t1 |
| chr6.1 | exon | 10374263 | 10374322 | 10374263 | ID=MS.gene87922.t1.exon9;Parent=MS.gene87922.t1 |
| chr6.1 | CDS | 10374263 | 10374322 | 10374263 | ID=cds.MS.gene87922.t1;Parent=MS.gene87922.t1 |
| chr6.1 | exon | 10373307 | 10373387 | 10373307 | ID=MS.gene87922.t1.exon10;Parent=MS.gene87922.t1 |
| chr6.1 | CDS | 10373307 | 10373387 | 10373307 | ID=cds.MS.gene87922.t1;Parent=MS.gene87922.t1 |
| chr6.1 | exon | 10372439 | 10372492 | 10372439 | ID=MS.gene87922.t1.exon11;Parent=MS.gene87922.t1 |
| chr6.1 | CDS | 10372439 | 10372492 | 10372439 | ID=cds.MS.gene87922.t1;Parent=MS.gene87922.t1 |
| Gene Sequence |
| Protein sequence |
