Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73078.t1 | XP_003598777.1 | 78.7 | 676 | 142 | 2 | 1 | 675 | 1 | 675 | 2.30E-270 | 941.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73078.t1 | A0A1P8BH59 | 59.7 | 72 | 29 | 0 | 580 | 651 | 1412 | 1483 | 1.1e-15 | 86.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73078.t1 | G7IW85 | 78.7 | 676 | 142 | 2 | 1 | 675 | 1 | 675 | 1.6e-270 | 941.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene73078 | 0.859242 | 4.74E-63 | -1.69E-46 |
| MS.gene04918 | MS.gene73078 | 0.825251 | 5.19E-54 | -1.69E-46 |
| MS.gene04919 | MS.gene73078 | 0.803267 | 3.78E-49 | -1.69E-46 |
| MS.gene04920 | MS.gene73078 | 0.817815 | 2.71E-52 | -1.69E-46 |
| MS.gene049254 | MS.gene73078 | 0.812418 | 4.28E-51 | -1.69E-46 |
| MS.gene049350 | MS.gene73078 | 0.824989 | 5.99E-54 | -1.69E-46 |
| MS.gene049368 | MS.gene73078 | 0.843693 | 1.20E-58 | -1.69E-46 |
| MS.gene049419 | MS.gene73078 | -0.814435 | 1.54E-51 | -1.69E-46 |
| MS.gene049499 | MS.gene73078 | 0.863089 | 3.19E-64 | -1.69E-46 |
| MS.gene049585 | MS.gene73078 | 0.82489 | 6.32E-54 | -1.69E-46 |
| MS.gene049711 | MS.gene73078 | 0.813581 | 2.38E-51 | -1.69E-46 |
| MS.gene049807 | MS.gene73078 | 0.804767 | 1.84E-49 | -1.69E-46 |
| MS.gene049983 | MS.gene73078 | 0.842267 | 2.87E-58 | -1.69E-46 |
| MS.gene050300 | MS.gene73078 | -0.812286 | 4.58E-51 | -1.69E-46 |
| MS.gene050359 | MS.gene73078 | 0.801858 | 7.39E-49 | -1.69E-46 |
| MS.gene050414 | MS.gene73078 | 0.818219 | 2.20E-52 | -1.69E-46 |
| MS.gene050646 | MS.gene73078 | 0.833414 | 5.41E-56 | -1.69E-46 |
| MS.gene050654 | MS.gene73078 | 0.82979 | 4.23E-55 | -1.69E-46 |
| MS.gene050941 | MS.gene73078 | 0.825728 | 4.00E-54 | -1.69E-46 |
| MS.gene051279 | MS.gene73078 | 0.805052 | 1.61E-49 | -1.69E-46 |
| MS.gene051332 | MS.gene73078 | 0.801464 | 8.90E-49 | -1.69E-46 |
| MS.gene051490 | MS.gene73078 | 0.80378 | 2.96E-49 | -1.69E-46 |
| MS.gene051676 | MS.gene73078 | 0.820961 | 5.20E-53 | -1.69E-46 |
| MS.gene052016 | MS.gene73078 | 0.820392 | 7.03E-53 | -1.69E-46 |
| MS.gene052168 | MS.gene73078 | 0.814161 | 1.77E-51 | -1.69E-46 |
| MS.gene052307 | MS.gene73078 | 0.831945 | 1.25E-55 | -1.69E-46 |
| MS.gene052335 | MS.gene73078 | 0.803203 | 3.90E-49 | -1.69E-46 |
| MS.gene052463 | MS.gene73078 | 0.855993 | 4.34E-62 | -1.69E-46 |
| MS.gene052493 | MS.gene73078 | 0.802928 | 4.45E-49 | -1.69E-46 |
| MS.gene052516 | MS.gene73078 | 0.805683 | 1.19E-49 | -1.69E-46 |
| MS.gene052518 | MS.gene73078 | 0.851897 | 6.58E-61 | -1.69E-46 |
| MS.gene052534 | MS.gene73078 | 0.804536 | 2.06E-49 | -1.69E-46 |
| MS.gene052609 | MS.gene73078 | 0.835192 | 1.94E-56 | -1.69E-46 |
| MS.gene052674 | MS.gene73078 | 0.800403 | 1.47E-48 | -1.69E-46 |
| MS.gene052675 | MS.gene73078 | 0.804541 | 2.06E-49 | -1.69E-46 |
| MS.gene052677 | MS.gene73078 | 0.801882 | 7.30E-49 | -1.69E-46 |
| MS.gene052932 | MS.gene73078 | 0.811011 | 8.66E-51 | -1.69E-46 |
| MS.gene053079 | MS.gene73078 | 0.806623 | 7.52E-50 | -1.69E-46 |
| MS.gene05350 | MS.gene73078 | 0.821852 | 3.24E-53 | -1.69E-46 |
| MS.gene053726 | MS.gene73078 | 0.802174 | 6.36E-49 | -1.69E-46 |
| MS.gene05423 | MS.gene73078 | 0.819252 | 1.28E-52 | -1.69E-46 |
| MS.gene054479 | MS.gene73078 | 0.830464 | 2.89E-55 | -1.69E-46 |
| MS.gene05454 | MS.gene73078 | 0.810641 | 1.04E-50 | -1.69E-46 |
| MS.gene05458 | MS.gene73078 | 0.835142 | 1.99E-56 | -1.69E-46 |
| MS.gene054641 | MS.gene73078 | 0.835026 | 2.13E-56 | -1.69E-46 |
| MS.gene054651 | MS.gene73078 | 0.810574 | 1.08E-50 | -1.69E-46 |
| MS.gene054709 | MS.gene73078 | 0.811542 | 6.65E-51 | -1.69E-46 |
| MS.gene054772 | MS.gene73078 | 0.80708 | 6.02E-50 | -1.69E-46 |
| MS.gene054892 | MS.gene73078 | 0.823286 | 1.50E-53 | -1.69E-46 |
| MS.gene054893 | MS.gene73078 | 0.828908 | 6.92E-55 | -1.69E-46 |
| MS.gene055141 | MS.gene73078 | 0.802631 | 5.12E-49 | -1.69E-46 |
| MS.gene055313 | MS.gene73078 | 0.807912 | 4.01E-50 | -1.69E-46 |
| MS.gene055327 | MS.gene73078 | 0.802842 | 4.63E-49 | -1.69E-46 |
| MS.gene055386 | MS.gene73078 | 0.801595 | 8.36E-49 | -1.69E-46 |
| MS.gene055469 | MS.gene73078 | 0.816359 | 5.76E-52 | -1.69E-46 |
| MS.gene055513 | MS.gene73078 | 0.848342 | 6.52E-60 | -1.69E-46 |
| MS.gene055724 | MS.gene73078 | 0.807104 | 5.95E-50 | -1.69E-46 |
| MS.gene055864 | MS.gene73078 | 0.860672 | 1.75E-63 | -1.69E-46 |
| MS.gene055926 | MS.gene73078 | 0.815701 | 8.08E-52 | -1.69E-46 |
| MS.gene056045 | MS.gene73078 | 0.810112 | 1.36E-50 | -1.69E-46 |
| MS.gene056174 | MS.gene73078 | 0.828956 | 6.74E-55 | -1.69E-46 |
| MS.gene056308 | MS.gene73078 | 0.819565 | 1.09E-52 | -1.69E-46 |
| MS.gene056699 | MS.gene73078 | 0.824483 | 7.88E-54 | -1.69E-46 |
| MS.gene05686 | MS.gene73078 | 0.825409 | 4.76E-54 | -1.69E-46 |
| MS.gene05690 | MS.gene73078 | 0.839105 | 1.93E-57 | -1.69E-46 |
| MS.gene057102 | MS.gene73078 | 0.831664 | 1.47E-55 | -1.69E-46 |
| MS.gene057208 | MS.gene73078 | 0.813299 | 2.75E-51 | -1.69E-46 |
| MS.gene057598 | MS.gene73078 | 0.814116 | 1.82E-51 | -1.69E-46 |
| MS.gene058010 | MS.gene73078 | 0.812822 | 3.49E-51 | -1.69E-46 |
| MS.gene058081 | MS.gene73078 | 0.822205 | 2.68E-53 | -1.69E-46 |
| MS.gene058159 | MS.gene73078 | 0.836384 | 9.66E-57 | -1.69E-46 |
| MS.gene058289 | MS.gene73078 | 0.80505 | 1.61E-49 | -1.69E-46 |
| MS.gene058292 | MS.gene73078 | 0.800412 | 1.46E-48 | -1.69E-46 |
| MS.gene058429 | MS.gene73078 | 0.821689 | 3.53E-53 | -1.69E-46 |
| MS.gene058836 | MS.gene73078 | 0.827432 | 1.57E-54 | -1.69E-46 |
| MS.gene05897 | MS.gene73078 | 0.847887 | 8.70E-60 | -1.69E-46 |
| MS.gene059144 | MS.gene73078 | 0.802986 | 4.32E-49 | -1.69E-46 |
| MS.gene059170 | MS.gene73078 | 0.827469 | 1.54E-54 | -1.69E-46 |
| MS.gene059283 | MS.gene73078 | 0.826307 | 2.91E-54 | -1.69E-46 |
| MS.gene059439 | MS.gene73078 | 0.838333 | 3.06E-57 | -1.69E-46 |
| MS.gene05952 | MS.gene73078 | 0.804058 | 2.59E-49 | -1.69E-46 |
| MS.gene059570 | MS.gene73078 | 0.808754 | 2.65E-50 | -1.69E-46 |
| MS.gene05985 | MS.gene73078 | -0.81466 | 1.38E-51 | -1.69E-46 |
| MS.gene060085 | MS.gene73078 | 0.806655 | 7.40E-50 | -1.69E-46 |
| MS.gene06010 | MS.gene73078 | 0.801217 | 9.99E-49 | -1.69E-46 |
| MS.gene060259 | MS.gene73078 | 0.814846 | 1.25E-51 | -1.69E-46 |
| MS.gene060435 | MS.gene73078 | 0.823355 | 1.45E-53 | -1.69E-46 |
| MS.gene06052 | MS.gene73078 | 0.816441 | 5.52E-52 | -1.69E-46 |
| MS.gene060777 | MS.gene73078 | 0.80917 | 2.16E-50 | -1.69E-46 |
| MS.gene060785 | MS.gene73078 | 0.843303 | 1.52E-58 | -1.69E-46 |
| MS.gene060850 | MS.gene73078 | 0.800365 | 1.49E-48 | -1.69E-46 |
| MS.gene06089 | MS.gene73078 | 0.813525 | 2.45E-51 | -1.69E-46 |
| MS.gene060932 | MS.gene73078 | 0.85269 | 3.91E-61 | -1.69E-46 |
| MS.gene060933 | MS.gene73078 | 0.848368 | 6.41E-60 | -1.69E-46 |
| MS.gene060935 | MS.gene73078 | 0.854289 | 1.36E-61 | -1.69E-46 |
| MS.gene060937 | MS.gene73078 | 0.864654 | 1.04E-64 | -1.69E-46 |
| MS.gene061100 | MS.gene73078 | 0.858992 | 5.63E-63 | -1.69E-46 |
| MS.gene061160 | MS.gene73078 | 0.806988 | 6.30E-50 | -1.69E-46 |
| MS.gene061188 | MS.gene73078 | 0.832701 | 8.13E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73078.t1 | MTR_3g020820 | 90.680 | 676 | 61 | 2 | 1 | 675 | 1 | 675 | 0.0 | 1191 |
| MS.gene73078.t1 | MTR_4g071030 | 38.384 | 792 | 307 | 33 | 1 | 674 | 1 | 729 | 2.97e-107 | 342 |
| MS.gene73078.t1 | MTR_7g090500 | 61.111 | 90 | 34 | 1 | 564 | 653 | 1210 | 1298 | 4.71e-26 | 115 |
| MS.gene73078.t1 | MTR_7g090500 | 41.954 | 174 | 85 | 5 | 82 | 241 | 41 | 212 | 6.15e-25 | 111 |
| MS.gene73078.t1 | MTR_7g090500 | 47.959 | 98 | 47 | 2 | 559 | 653 | 788 | 884 | 3.28e-17 | 86.7 |
| MS.gene73078.t1 | MTR_7g090500 | 46.939 | 98 | 48 | 2 | 559 | 653 | 440 | 536 | 6.03e-16 | 82.8 |
| MS.gene73078.t1 | MTR_7g075800 | 40.260 | 154 | 72 | 7 | 97 | 244 | 349 | 488 | 9.59e-15 | 78.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73078.t1 | AT2G38800 | 50.000 | 172 | 62 | 6 | 85 | 247 | 64 | 220 | 1.76e-34 | 139 |
| MS.gene73078.t1 | AT3G54570 | 58.333 | 84 | 31 | 1 | 571 | 654 | 338 | 417 | 2.09e-22 | 100 |
| MS.gene73078.t1 | AT3G54570 | 30.982 | 326 | 140 | 12 | 79 | 401 | 36 | 279 | 5.87e-20 | 93.6 |
| MS.gene73078.t1 | AT5G04020 | 54.545 | 88 | 39 | 1 | 564 | 651 | 1397 | 1483 | 1.29e-19 | 94.7 |
| MS.gene73078.t1 | AT5G04020 | 47.727 | 88 | 45 | 1 | 564 | 651 | 1143 | 1229 | 3.56e-17 | 86.7 |
| MS.gene73078.t1 | AT5G04020 | 47.222 | 72 | 28 | 1 | 592 | 653 | 521 | 592 | 1.91e-11 | 68.2 |
| MS.gene73078.t1 | AT5G04020 | 54.545 | 88 | 39 | 1 | 564 | 651 | 1397 | 1483 | 1.29e-19 | 94.7 |
| MS.gene73078.t1 | AT5G04020 | 47.727 | 88 | 45 | 1 | 564 | 651 | 1143 | 1229 | 3.56e-17 | 86.7 |
| MS.gene73078.t1 | AT5G04020 | 47.222 | 72 | 28 | 1 | 592 | 653 | 521 | 592 | 1.91e-11 | 68.2 |
Find 85 sgRNAs with CRISPR-Local
Find 110 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACAAAGACTACTAGCTTTA+AGG | 0.115739 | 3.2:-10761658 | MS.gene73078:CDS |
| CTGATTTAACATGTTCATTT+TGG | 0.128866 | 3.2:+10762093 | None:intergenic |
| TATACTTATTGTTCTCTTAA+TGG | 0.173869 | 3.2:-10761495 | MS.gene73078:CDS |
| TCAAAAGCTGAATCCTTATC+TGG | 0.223425 | 3.2:-10760907 | MS.gene73078:CDS |
| CTTGTTGGCTTCATGTAATT+TGG | 0.232774 | 3.2:+10761840 | None:intergenic |
| AAAAGGAAAAGGTTGATTTA+AGG | 0.235181 | 3.2:-10760330 | MS.gene73078:CDS |
| ATAGATGATTTCTTCAGATT+TGG | 0.284198 | 3.2:+10760792 | None:intergenic |
| AATCTATGGTAATGAGTTAA+AGG | 0.296459 | 3.2:-10761262 | MS.gene73078:CDS |
| GATTTCTTCATTGAAATCTA+TGG | 0.300238 | 3.2:-10761276 | MS.gene73078:CDS |
| GATCTTATCAAAGCCTTTGT+AGG | 0.302558 | 3.2:+10761708 | None:intergenic |
| CTTAAATGTGACTCACATTT+CGG | 0.313570 | 3.2:+10760172 | None:intergenic |
| TCATCTCATCAACACTTAGA+TGG | 0.319701 | 3.2:-10762035 | MS.gene73078:CDS |
| AGCAATGAGACTCAAGAAAT+TGG | 0.324372 | 3.2:-10760712 | MS.gene73078:CDS |
| TTTGCATGTGAACTGCTTGT+TGG | 0.325066 | 3.2:+10761825 | None:intergenic |
| TGATTCGGAATCCGAAAATA+TGG | 0.327207 | 3.2:-10761010 | MS.gene73078:CDS |
| AAGATCAACTAGTTTGAGTT+TGG | 0.327392 | 3.2:-10761691 | MS.gene73078:CDS |
| CTCAACCTCTTCAAGAAAAT+CGG | 0.331243 | 3.2:+10761203 | None:intergenic |
| TGTTCTCATCTTCGTTCTCT+TGG | 0.352163 | 3.2:+10760593 | None:intergenic |
| TGCTTCGAATTTCATCTTCA+AGG | 0.353332 | 3.2:+10760731 | None:intergenic |
| TCACATTTAAGAAACAAATC+AGG | 0.363401 | 3.2:-10760160 | MS.gene73078:CDS |
| AATCAAATATTTCATTTGCC+AGG | 0.365507 | 3.2:+10760653 | None:intergenic |
| ATGTAATTTGGTGAACCATC+TGG | 0.372192 | 3.2:+10761852 | None:intergenic |
| AGGAAAACACAAGAGAGAAT+TGG | 0.404742 | 3.2:-10760456 | MS.gene73078:CDS |
| GGTACTAATGGAAGGAAATT+CGG | 0.412268 | 3.2:+10760361 | None:intergenic |
| AGAGAAAGAATGCAGAGGAT+TGG | 0.414057 | 3.2:-10760291 | MS.gene73078:CDS |
| GTCTCATGAAGAAATGAGTA+TGG | 0.420379 | 3.2:-10761061 | MS.gene73078:CDS |
| GCCACTTTCTTCTTCCTAGC+AGG | 0.425788 | 3.2:+10760226 | None:intergenic |
| TTAAAGGATGCAAAATCAAC+AGG | 0.436041 | 3.2:-10761246 | MS.gene73078:CDS |
| TCTTCAATCTTCACCTTCAC+CGG | 0.446622 | 3.2:-10761940 | MS.gene73078:CDS |
| TTCCACCAGGATTAAGCATA+AGG | 0.448625 | 3.2:+10761556 | None:intergenic |
| CCTGGTGGAACTGAGTTAGA+AGG | 0.450702 | 3.2:-10761543 | MS.gene73078:CDS |
| CCCTCGTGGTCGAAGATTGA+AGG | 0.451781 | 3.2:-10761382 | MS.gene73078:CDS |
| TTTCATTTGCCAGGGAAGAT+TGG | 0.452112 | 3.2:+10760662 | None:intergenic |
| AGAAGGAACTTCAGTTATGA+AGG | 0.453073 | 3.2:-10761526 | MS.gene73078:CDS |
| ATCAAAGATATTCAAGATCA+AGG | 0.463943 | 3.2:-10760517 | MS.gene73078:CDS |
| TAACAATGATGATTCTGATT+CGG | 0.471750 | 3.2:-10761025 | MS.gene73078:CDS |
| CTCATGATGTATCAGTTACA+TGG | 0.480491 | 3.2:-10760879 | MS.gene73078:CDS |
| GGAAGGAAATTCGGCTCTCT+CGG | 0.486466 | 3.2:+10760370 | None:intergenic |
| TGATTTAAGGCATCAAATGA+TGG | 0.487620 | 3.2:-10760317 | MS.gene73078:CDS |
| GAAAATGATGGCATAGAAGT+AGG | 0.489902 | 3.2:-10761150 | MS.gene73078:CDS |
| AAACTCATCATGTCATTATG+AGG | 0.490510 | 3.2:+10760757 | None:intergenic |
| TCATACCTTATGCTTAATCC+TGG | 0.495217 | 3.2:-10761561 | MS.gene73078:CDS |
| ATGGCAGTTTCATCATATGA+AGG | 0.496697 | 3.2:+10761300 | None:intergenic |
| AGGTCTACAATTGAAAATGA+TGG | 0.500117 | 3.2:-10761162 | MS.gene73078:CDS |
| AAGCAGTTCACATGCAAAGA+AGG | 0.509530 | 3.2:-10761820 | MS.gene73078:CDS |
| TGTCATCATAATAGCTGCTA+AGG | 0.511369 | 3.2:+10760845 | None:intergenic |
| AAAGAATGCAGAGGATTGGA+TGG | 0.516505 | 3.2:-10760287 | MS.gene73078:CDS |
| CCTTCTAACTCAGTTCCACC+AGG | 0.518876 | 3.2:+10761543 | None:intergenic |
| GGTCGAAGATTGAAGGCTAC+TGG | 0.519739 | 3.2:-10761375 | MS.gene73078:CDS |
| CACATTTAAGAAACAAATCA+GGG | 0.535743 | 3.2:-10760159 | MS.gene73078:CDS |
| ATCATGAGAACTCCCAGATA+AGG | 0.536602 | 3.2:+10760894 | None:intergenic |
| AGAAGTAGGATTTCTTAAAG+AGG | 0.536795 | 3.2:-10761136 | MS.gene73078:CDS |
| CAAAAGCTGAATCCTTATCT+GGG | 0.541306 | 3.2:-10760906 | MS.gene73078:CDS |
| ATCAAATATTTCATTTGCCA+GGG | 0.543883 | 3.2:+10760654 | None:intergenic |
| GGATTAAGCATAAGGTATGA+AGG | 0.549187 | 3.2:+10761564 | None:intergenic |
| AAGTTGTGTAATTGATGAAG+AGG | 0.552210 | 3.2:-10760476 | MS.gene73078:CDS |
| TTTCGGTATTACAGTTTCGA+AGG | 0.554157 | 3.2:+10760189 | None:intergenic |
| ATGTAGATCAACAAAGTAGT+TGG | 0.555361 | 3.2:-10761083 | MS.gene73078:CDS |
| AACAAATCAGGGTTTGCTCA+TGG | 0.556841 | 3.2:-10760148 | MS.gene73078:CDS |
| AGGATCATGAGGATATCATC+AGG | 0.558154 | 3.2:-10761182 | MS.gene73078:CDS |
| AATCAAGCTTTCTGATCTTG+AGG | 0.564641 | 3.2:-10761964 | MS.gene73078:CDS |
| TACCTTATGCTTAATCCTGG+TGG | 0.569094 | 3.2:-10761558 | MS.gene73078:CDS |
| CGGTTGCTACCAATCTTCCC+TGG | 0.571699 | 3.2:-10760671 | MS.gene73078:CDS |
| GTAGAACTCGGAACCGGTGA+AGG | 0.578258 | 3.2:+10761927 | None:intergenic |
| AAGATGATGACGATAAAATG+AGG | 0.584211 | 3.2:-10760402 | MS.gene73078:CDS |
| GAAGACATAAAAGTCTTCAA+AGG | 0.584823 | 3.2:+10761447 | None:intergenic |
| TTCATATGATGAAACTGCCA+TGG | 0.585852 | 3.2:-10761298 | MS.gene73078:CDS |
| TTGGTAGCAACCGATGTCAT+CGG | 0.589354 | 3.2:+10760681 | None:intergenic |
| TTAACAAGCTTGCTCCTGCT+AGG | 0.594927 | 3.2:-10760240 | MS.gene73078:CDS |
| GATATTCAAGATCAAGGCAA+AGG | 0.596583 | 3.2:-10760511 | MS.gene73078:CDS |
| GACATAAAAGTCTTCAAAGG+AGG | 0.599107 | 3.2:+10761450 | None:intergenic |
| TGAAGAGGTTGAGGATCATG+AGG | 0.600460 | 3.2:-10761193 | MS.gene73078:CDS |
| TCTGGAGTTGTTCTCACCAA+AGG | 0.600740 | 3.2:+10761870 | None:intergenic |
| TTGGTGAGAACAACTCCAGA+TGG | 0.604224 | 3.2:-10761867 | MS.gene73078:CDS |
| GGTTACTCCTCCGATGACAT+CGG | 0.610168 | 3.2:-10760691 | MS.gene73078:CDS |
| AACACAAGAGAGAATTGGAA+AGG | 0.616964 | 3.2:-10760451 | MS.gene73078:CDS |
| GCCTTCAATCTTCGACCACG+AGG | 0.619143 | 3.2:+10761381 | None:intergenic |
| CTCGAATCTCTGAGCCCTCG+TGG | 0.619870 | 3.2:-10761396 | MS.gene73078:CDS |
| TCCTGCTAGGAAGAAGAAAG+TGG | 0.632688 | 3.2:-10760227 | MS.gene73078:CDS |
| GGATGAGAGAAAGAATGCAG+AGG | 0.637454 | 3.2:-10760296 | MS.gene73078:CDS |
| GATTTCAATGAAGAAATCCA+TGG | 0.638428 | 3.2:+10761281 | None:intergenic |
| AAGGTGTGATTCGAAGAAAG+AGG | 0.642021 | 3.2:-10760432 | MS.gene73078:CDS |
| AGATGAGAACATCAATAGAG+AGG | 0.642510 | 3.2:-10760581 | MS.gene73078:CDS |
| GTAGCAACCGATGTCATCGG+AGG | 0.692341 | 3.2:+10760684 | None:intergenic |
| CCTTCAATCTTCGACCACGA+GGG | 0.692597 | 3.2:+10761382 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATACTTATTGTTCTCTTAA+TGG | - | chr3.2:10760756-10760775 | MS.gene73078:CDS | 20.0% |
| !!! | ATATGAAGGTTTTAAATCAA+AGG | + | chr3.2:10760940-10760959 | None:intergenic | 20.0% |
| ! | AATCAAATATTTCATTTGCC+AGG | + | chr3.2:10761601-10761620 | None:intergenic | 25.0% |
| ! | ATAGATGATTTCTTCAGATT+TGG | + | chr3.2:10761462-10761481 | None:intergenic | 25.0% |
| ! | ATCAAAGATATTCAAGATCA+AGG | - | chr3.2:10761734-10761753 | MS.gene73078:CDS | 25.0% |
| ! | ATCAAATATTTCATTTGCCA+GGG | + | chr3.2:10761600-10761619 | None:intergenic | 25.0% |
| ! | ATTAGTACCTGAAAAAGAAA+AGG | - | chr3.2:10761904-10761923 | MS.gene73078:CDS | 25.0% |
| ! | CACATTTAAGAAACAAATCA+GGG | - | chr3.2:10762092-10762111 | MS.gene73078:CDS | 25.0% |
| ! | GATTTCTTCATTGAAATCTA+TGG | - | chr3.2:10760975-10760994 | MS.gene73078:CDS | 25.0% |
| ! | TAACAATGATGATTCTGATT+CGG | - | chr3.2:10761226-10761245 | MS.gene73078:CDS | 25.0% |
| ! | TATATGTAAAAAGCCTACAA+AGG | - | chr3.2:10760530-10760549 | MS.gene73078:CDS | 25.0% |
| ! | TCACATTTAAGAAACAAATC+AGG | - | chr3.2:10762091-10762110 | MS.gene73078:CDS | 25.0% |
| !! | AAAAGGAAAAGGTTGATTTA+AGG | - | chr3.2:10761921-10761940 | MS.gene73078:CDS | 25.0% |
| !! | AATCTATGGTAATGAGTTAA+AGG | - | chr3.2:10760989-10761008 | MS.gene73078:CDS | 25.0% |
| !! | CTGATTTAACATGTTCATTT+TGG | + | chr3.2:10760161-10760180 | None:intergenic | 25.0% |
| !! | TGTGATGAAGAACAATTTTA+TGG | - | chr3.2:10761266-10761285 | MS.gene73078:CDS | 25.0% |
| !!! | TTTTCTTTTTCAGGTACTAA+TGG | + | chr3.2:10761905-10761924 | None:intergenic | 25.0% |
| AAACTCATCATGTCATTATG+AGG | + | chr3.2:10761497-10761516 | None:intergenic | 30.0% | |
| AAGTTGTGTAATTGATGAAG+AGG | - | chr3.2:10761775-10761794 | MS.gene73078:CDS | 30.0% | |
| ACCTGAAAAAGAAAAGGAAA+AGG | - | chr3.2:10761910-10761929 | MS.gene73078:CDS | 30.0% | |
| AGAAGTAGGATTTCTTAAAG+AGG | - | chr3.2:10761115-10761134 | MS.gene73078:CDS | 30.0% | |
| AGGTCTACAATTGAAAATGA+TGG | - | chr3.2:10761089-10761108 | MS.gene73078:CDS | 30.0% | |
| AGTCTAAGAAAAACTCAATC+TGG | - | chr3.2:10760468-10760487 | MS.gene73078:CDS | 30.0% | |
| ATGTAGATCAACAAAGTAGT+TGG | - | chr3.2:10761168-10761187 | MS.gene73078:CDS | 30.0% | |
| CTTAAATGTGACTCACATTT+CGG | + | chr3.2:10762082-10762101 | None:intergenic | 30.0% | |
| GAAGACATAAAAGTCTTCAA+AGG | + | chr3.2:10760807-10760826 | None:intergenic | 30.0% | |
| GATTTCAATGAAGAAATCCA+TGG | + | chr3.2:10760973-10760992 | None:intergenic | 30.0% | |
| TTAAAGGATGCAAAATCAAC+AGG | - | chr3.2:10761005-10761024 | MS.gene73078:CDS | 30.0% | |
| ! | AAGATCAACTAGTTTGAGTT+TGG | - | chr3.2:10760560-10760579 | MS.gene73078:CDS | 30.0% |
| ! | AAGATGATGACGATAAAATG+AGG | - | chr3.2:10761849-10761868 | MS.gene73078:CDS | 30.0% |
| ! | ACCTTTTCCTTTTCTTTTTC+AGG | + | chr3.2:10761914-10761933 | None:intergenic | 30.0% |
| ! | TGATTTAAGGCATCAAATGA+TGG | - | chr3.2:10761934-10761953 | MS.gene73078:CDS | 30.0% |
| ! | TTCATCACACTCCATATTTT+CGG | + | chr3.2:10761255-10761274 | None:intergenic | 30.0% |
| !! | AAAAACCGATTTTCTTGAAG+AGG | - | chr3.2:10761043-10761062 | MS.gene73078:CDS | 30.0% |
| !!! | TCAAAGAAAGGTTTTAAGCA+AGG | - | chr3.2:10760128-10760147 | MS.gene73078:CDS | 30.0% |
| AACACAAGAGAGAATTGGAA+AGG | - | chr3.2:10761800-10761819 | MS.gene73078:CDS | 35.0% | |
| AATCAAGCTTTCTGATCTTG+AGG | - | chr3.2:10760287-10760306 | MS.gene73078:CDS | 35.0% | |
| AGAAGGAACTTCAGTTATGA+AGG | - | chr3.2:10760725-10760744 | MS.gene73078:CDS | 35.0% | |
| AGATGAGAACATCAATAGAG+AGG | - | chr3.2:10761670-10761689 | MS.gene73078:CDS | 35.0% | |
| AGCAATGAGACTCAAGAAAT+TGG | - | chr3.2:10761539-10761558 | MS.gene73078:CDS | 35.0% | |
| AGGAAAACACAAGAGAGAAT+TGG | - | chr3.2:10761795-10761814 | MS.gene73078:CDS | 35.0% | |
| ATGGCAGTTTCATCATATGA+AGG | + | chr3.2:10760954-10760973 | None:intergenic | 35.0% | |
| CAAAAGCTGAATCCTTATCT+GGG | - | chr3.2:10761345-10761364 | MS.gene73078:CDS | 35.0% | |
| CTCAACCTCTTCAAGAAAAT+CGG | + | chr3.2:10761051-10761070 | None:intergenic | 35.0% | |
| CTCATGATGTATCAGTTACA+TGG | - | chr3.2:10761372-10761391 | MS.gene73078:CDS | 35.0% | |
| GAAAATGATGGCATAGAAGT+AGG | - | chr3.2:10761101-10761120 | MS.gene73078:CDS | 35.0% | |
| GACATAAAAGTCTTCAAAGG+AGG | + | chr3.2:10760804-10760823 | None:intergenic | 35.0% | |
| GATATTCAAGATCAAGGCAA+AGG | - | chr3.2:10761740-10761759 | MS.gene73078:CDS | 35.0% | |
| GATCTTATCAAAGCCTTTGT+AGG | + | chr3.2:10760546-10760565 | None:intergenic | 35.0% | |
| GGATTAAGCATAAGGTATGA+AGG | + | chr3.2:10760690-10760709 | None:intergenic | 35.0% | |
| GGTACTAATGGAAGGAAATT+CGG | + | chr3.2:10761893-10761912 | None:intergenic | 35.0% | |
| GTCTCATGAAGAAATGAGTA+TGG | - | chr3.2:10761190-10761209 | MS.gene73078:CDS | 35.0% | |
| TCAAAAGCTGAATCCTTATC+TGG | - | chr3.2:10761344-10761363 | MS.gene73078:CDS | 35.0% | |
| TCATACCTTATGCTTAATCC+TGG | - | chr3.2:10760690-10760709 | MS.gene73078:CDS | 35.0% | |
| TCATCTCATCAACACTTAGA+TGG | - | chr3.2:10760216-10760235 | MS.gene73078:CDS | 35.0% | |
| TGCTTCGAATTTCATCTTCA+AGG | + | chr3.2:10761523-10761542 | None:intergenic | 35.0% | |
| TGTCATCATAATAGCTGCTA+AGG | + | chr3.2:10761409-10761428 | None:intergenic | 35.0% | |
| TTCATATGATGAAACTGCCA+TGG | - | chr3.2:10760953-10760972 | MS.gene73078:CDS | 35.0% | |
| TTTCGGTATTACAGTTTCGA+AGG | + | chr3.2:10762065-10762084 | None:intergenic | 35.0% | |
| ! | ATGTAATTTGGTGAACCATC+TGG | + | chr3.2:10760402-10760421 | None:intergenic | 35.0% |
| ! | TGAAGACTTTTATGTCTTCG+AGG | - | chr3.2:10760808-10760827 | MS.gene73078:CDS | 35.0% |
| ! | TGATTCGGAATCCGAAAATA+TGG | - | chr3.2:10761241-10761260 | MS.gene73078:CDS | 35.0% |
| ! | TGTCTCGTCAACATTTTCTT+CGG | + | chr3.2:10761636-10761655 | None:intergenic | 35.0% |
| !! | CTTGTTGGCTTCATGTAATT+TGG | + | chr3.2:10760414-10760433 | None:intergenic | 35.0% |
| !! | GACAAAGACTACTAGCTTTA+AGG | - | chr3.2:10760593-10760612 | MS.gene73078:CDS | 35.0% |
| !!! | AGAAAGGTTTTAAGCAAGGT+TGG | - | chr3.2:10760132-10760151 | MS.gene73078:CDS | 35.0% |
| !!! | CTTTTTCAGGTACTAATGGA+AGG | + | chr3.2:10761901-10761920 | None:intergenic | 35.0% |
| !!! | GTAGCTCTTGTTGATTTTCT+AGG | + | chr3.2:10760636-10760655 | None:intergenic | 35.0% |
| AAAGAATGCAGAGGATTGGA+TGG | - | chr3.2:10761964-10761983 | MS.gene73078:CDS | 40.0% | |
| AAGCAGTTCACATGCAAAGA+AGG | - | chr3.2:10760431-10760450 | MS.gene73078:CDS | 40.0% | |
| AAGGTGTGATTCGAAGAAAG+AGG | - | chr3.2:10761819-10761838 | MS.gene73078:CDS | 40.0% | |
| ACCACAAAAACAGCATCCTT+TGG | - | chr3.2:10760365-10760384 | MS.gene73078:CDS | 40.0% | |
| AGAGAAAGAATGCAGAGGAT+TGG | - | chr3.2:10761960-10761979 | MS.gene73078:CDS | 40.0% | |
| AGGATCATGAGGATATCATC+AGG | - | chr3.2:10761069-10761088 | MS.gene73078:CDS | 40.0% | |
| ATCATGAGAACTCCCAGATA+AGG | + | chr3.2:10761360-10761379 | None:intergenic | 40.0% | |
| TACCTTATGCTTAATCCTGG+TGG | - | chr3.2:10760693-10760712 | MS.gene73078:CDS | 40.0% | |
| TCTTCAATCTTCACCTTCAC+CGG | - | chr3.2:10760311-10760330 | MS.gene73078:CDS | 40.0% | |
| TGTTCTCATCTTCGTTCTCT+TGG | + | chr3.2:10761661-10761680 | None:intergenic | 40.0% | |
| TTCCACCAGGATTAAGCATA+AGG | + | chr3.2:10760698-10760717 | None:intergenic | 40.0% | |
| TTTCATTTGCCAGGGAAGAT+TGG | + | chr3.2:10761592-10761611 | None:intergenic | 40.0% | |
| TTTGCATGTGAACTGCTTGT+TGG | + | chr3.2:10760429-10760448 | None:intergenic | 40.0% | |
| ! | AACAAATCAGGGTTTGCTCA+TGG | - | chr3.2:10762103-10762122 | MS.gene73078:CDS | 40.0% |
| ! | AGACTTTTATGTCTTCGAGG+AGG | - | chr3.2:10760811-10760830 | MS.gene73078:CDS | 40.0% |
| ! | CATTTTCTTCGGCATTTGTC+AGG | + | chr3.2:10761625-10761644 | None:intergenic | 40.0% |
| ! | CGATTTTCTTGAAGAGGTTG+AGG | - | chr3.2:10761049-10761068 | MS.gene73078:CDS | 40.0% |
| !! | GGTACTGCTTTTGTAGAACT+CGG | + | chr3.2:10760339-10760358 | None:intergenic | 40.0% |
| !!! | ACCAAAGGATGCTGTTTTTG+TGG | + | chr3.2:10760369-10760388 | None:intergenic | 40.0% |
| GGATGAGAGAAAGAATGCAG+AGG | - | chr3.2:10761955-10761974 | MS.gene73078:CDS | 45.0% | |
| TCCTGCTAGGAAGAAGAAAG+TGG | - | chr3.2:10762024-10762043 | MS.gene73078:CDS | 45.0% | |
| TGAAGAGGTTGAGGATCATG+AGG | - | chr3.2:10761058-10761077 | MS.gene73078:CDS | 45.0% | |
| TTGGTGAGAACAACTCCAGA+TGG | - | chr3.2:10760384-10760403 | MS.gene73078:CDS | 45.0% | |
| ! | GCTTTTGTAGAACTCGGAAC+CGG | + | chr3.2:10760333-10760352 | None:intergenic | 45.0% |
| ! | TCTGGAGTTGTTCTCACCAA+AGG | + | chr3.2:10760384-10760403 | None:intergenic | 45.0% |
| ! | TTAACAAGCTTGCTCCTGCT+AGG | - | chr3.2:10762011-10762030 | MS.gene73078:CDS | 45.0% |
| ! | TTGGTAGCAACCGATGTCAT+CGG | + | chr3.2:10761573-10761592 | None:intergenic | 45.0% |
| !!! | AGGATGCTGTTTTTGTGGTG+AGG | + | chr3.2:10760364-10760383 | None:intergenic | 45.0% |
| CCTGGTGGAACTGAGTTAGA+AGG | - | chr3.2:10760708-10760727 | MS.gene73078:CDS | 50.0% | |
| CCTTCAATCTTCGACCACGA+GGG | + | chr3.2:10760872-10760891 | None:intergenic | 50.0% | |
| CCTTCTAACTCAGTTCCACC+AGG | + | chr3.2:10760711-10760730 | None:intergenic | 50.0% | |
| GGAAGGAAATTCGGCTCTCT+CGG | + | chr3.2:10761884-10761903 | None:intergenic | 50.0% | |
| GGTCGAAGATTGAAGGCTAC+TGG | - | chr3.2:10760876-10760895 | MS.gene73078:CDS | 50.0% | |
| GGTTACTCCTCCGATGACAT+CGG | - | chr3.2:10761560-10761579 | MS.gene73078:CDS | 50.0% | |
| ! | GCCACTTTCTTCTTCCTAGC+AGG | + | chr3.2:10762028-10762047 | None:intergenic | 50.0% |
| !!! | TGCTGTTTTTGTGGTGAGGC+TGG | + | chr3.2:10760360-10760379 | None:intergenic | 50.0% |
| CCCTCGTGGTCGAAGATTGA+AGG | - | chr3.2:10760869-10760888 | MS.gene73078:CDS | 55.0% | |
| CGGTTGCTACCAATCTTCCC+TGG | - | chr3.2:10761580-10761599 | MS.gene73078:CDS | 55.0% | |
| GCCTTCAATCTTCGACCACG+AGG | + | chr3.2:10760873-10760892 | None:intergenic | 55.0% | |
| GTAGAACTCGGAACCGGTGA+AGG | + | chr3.2:10760327-10760346 | None:intergenic | 55.0% | |
| GTAGCAACCGATGTCATCGG+AGG | + | chr3.2:10761570-10761589 | None:intergenic | 55.0% | |
| CTCGAATCTCTGAGCCCTCG+TGG | - | chr3.2:10760855-10760874 | MS.gene73078:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 10760123 | 10762150 | 10760123 | ID=MS.gene73078 |
| chr3.2 | mRNA | 10760123 | 10762150 | 10760123 | ID=MS.gene73078.t1;Parent=MS.gene73078 |
| chr3.2 | exon | 10760123 | 10762150 | 10760123 | ID=MS.gene73078.t1.exon1;Parent=MS.gene73078.t1 |
| chr3.2 | CDS | 10760123 | 10762150 | 10760123 | ID=cds.MS.gene73078.t1;Parent=MS.gene73078.t1 |
| Gene Sequence |
| Protein sequence |
