Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94674.t1 | XP_013449157.1 | 97.7 | 176 | 3 | 1 | 1 | 175 | 118 | 293 | 1.40E-30 | 142.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94674.t1 | Q9SDW0 | 52.6 | 196 | 69 | 1 | 2 | 173 | 118 | 313 | 1.2e-09 | 64.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94674.t1 | A0A072U082 | 97.7 | 176 | 3 | 1 | 1 | 175 | 118 | 293 | 1.0e-30 | 142.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene94674 | 0.813844 | 2.08E-51 | -1.69E-46 |
| MS.gene04918 | MS.gene94674 | 0.885784 | 6.03E-72 | -1.69E-46 |
| MS.gene04919 | MS.gene94674 | 0.865207 | 6.98E-65 | -1.69E-46 |
| MS.gene04920 | MS.gene94674 | 0.885494 | 7.75E-72 | -1.69E-46 |
| MS.gene049254 | MS.gene94674 | 0.839886 | 1.21E-57 | -1.69E-46 |
| MS.gene049283 | MS.gene94674 | 0.862177 | 6.10E-64 | -1.69E-46 |
| MS.gene049350 | MS.gene94674 | 0.815258 | 1.01E-51 | -1.69E-46 |
| MS.gene049368 | MS.gene94674 | 0.838532 | 2.72E-57 | -1.69E-46 |
| MS.gene049419 | MS.gene94674 | -0.844515 | 7.21E-59 | -1.69E-46 |
| MS.gene049452 | MS.gene94674 | 0.817461 | 3.26E-52 | -1.69E-46 |
| MS.gene049499 | MS.gene94674 | 0.877034 | 8.68E-69 | -1.69E-46 |
| MS.gene049680 | MS.gene94674 | 0.803278 | 3.76E-49 | -1.69E-46 |
| MS.gene049711 | MS.gene94674 | 0.817102 | 3.93E-52 | -1.69E-46 |
| MS.gene049983 | MS.gene94674 | 0.882189 | 1.28E-70 | -1.69E-46 |
| MS.gene04998 | MS.gene94674 | 0.806784 | 6.95E-50 | -1.69E-46 |
| MS.gene050238 | MS.gene94674 | 0.806434 | 8.24E-50 | -1.69E-46 |
| MS.gene050300 | MS.gene94674 | -0.801442 | 8.99E-49 | -1.69E-46 |
| MS.gene050414 | MS.gene94674 | 0.852732 | 3.81E-61 | -1.69E-46 |
| MS.gene050571 | MS.gene94674 | 0.816121 | 6.51E-52 | -1.69E-46 |
| MS.gene050646 | MS.gene94674 | 0.842378 | 2.68E-58 | -1.69E-46 |
| MS.gene050654 | MS.gene94674 | 0.85094 | 1.23E-60 | -1.69E-46 |
| MS.gene050737 | MS.gene94674 | 0.801031 | 1.09E-48 | -1.69E-46 |
| MS.gene050941 | MS.gene94674 | 0.829196 | 5.89E-55 | -1.69E-46 |
| MS.gene050945 | MS.gene94674 | 0.818434 | 1.96E-52 | -1.69E-46 |
| MS.gene051003 | MS.gene94674 | 0.81607 | 6.68E-52 | -1.69E-46 |
| MS.gene051019 | MS.gene94674 | 0.819233 | 1.29E-52 | -1.69E-46 |
| MS.gene051043 | MS.gene94674 | 0.821556 | 3.79E-53 | -1.69E-46 |
| MS.gene051234 | MS.gene94674 | 0.824594 | 7.42E-54 | -1.69E-46 |
| MS.gene051275 | MS.gene94674 | 0.808709 | 2.71E-50 | -1.69E-46 |
| MS.gene051279 | MS.gene94674 | 0.807439 | 5.05E-50 | -1.69E-46 |
| MS.gene051332 | MS.gene94674 | 0.825275 | 5.12E-54 | -1.69E-46 |
| MS.gene051343 | MS.gene94674 | 0.808077 | 3.70E-50 | -1.69E-46 |
| MS.gene051363 | MS.gene94674 | 0.801316 | 9.54E-49 | -1.69E-46 |
| MS.gene051490 | MS.gene94674 | 0.812456 | 4.20E-51 | -1.69E-46 |
| MS.gene051541 | MS.gene94674 | 0.800447 | 1.43E-48 | -1.69E-46 |
| MS.gene051676 | MS.gene94674 | 0.825276 | 5.12E-54 | -1.69E-46 |
| MS.gene051699 | MS.gene94674 | 0.81504 | 1.13E-51 | -1.69E-46 |
| MS.gene051831 | MS.gene94674 | 0.801926 | 7.15E-49 | -1.69E-46 |
| MS.gene051832 | MS.gene94674 | 0.818765 | 1.65E-52 | -1.69E-46 |
| MS.gene051848 | MS.gene94674 | -0.808915 | 2.45E-50 | -1.69E-46 |
| MS.gene051852 | MS.gene94674 | 0.813599 | 2.36E-51 | -1.69E-46 |
| MS.gene05188 | MS.gene94674 | 0.803227 | 3.86E-49 | -1.69E-46 |
| MS.gene051902 | MS.gene94674 | 0.808571 | 2.90E-50 | -1.69E-46 |
| MS.gene051975 | MS.gene94674 | 0.829072 | 6.32E-55 | -1.69E-46 |
| MS.gene052016 | MS.gene94674 | 0.858272 | 9.23E-63 | -1.69E-46 |
| MS.gene052072 | MS.gene94674 | 0.815475 | 9.07E-52 | -1.69E-46 |
| MS.gene052118 | MS.gene94674 | 0.845866 | 3.11E-59 | -1.69E-46 |
| MS.gene052296 | MS.gene94674 | 0.817574 | 3.07E-52 | -1.69E-46 |
| MS.gene052307 | MS.gene94674 | 0.81485 | 1.25E-51 | -1.69E-46 |
| MS.gene052342 | MS.gene94674 | 0.820656 | 6.11E-53 | -1.69E-46 |
| MS.gene052463 | MS.gene94674 | 0.854306 | 1.34E-61 | -1.69E-46 |
| MS.gene052493 | MS.gene94674 | 0.811365 | 7.26E-51 | -1.69E-46 |
| MS.gene052518 | MS.gene94674 | 0.838568 | 2.66E-57 | -1.69E-46 |
| MS.gene052519 | MS.gene94674 | 0.811283 | 7.56E-51 | -1.69E-46 |
| MS.gene052533 | MS.gene94674 | 0.851919 | 6.48E-61 | -1.69E-46 |
| MS.gene052534 | MS.gene94674 | 0.83765 | 4.59E-57 | -1.69E-46 |
| MS.gene052582 | MS.gene94674 | 0.811598 | 6.46E-51 | -1.69E-46 |
| MS.gene052584 | MS.gene94674 | 0.80195 | 7.07E-49 | -1.69E-46 |
| MS.gene052685 | MS.gene94674 | 0.826188 | 3.11E-54 | -1.69E-46 |
| MS.gene052793 | MS.gene94674 | 0.826514 | 2.60E-54 | -1.69E-46 |
| MS.gene052855 | MS.gene94674 | 0.82569 | 4.09E-54 | -1.69E-46 |
| MS.gene052888 | MS.gene94674 | 0.811496 | 6.80E-51 | -1.69E-46 |
| MS.gene052889 | MS.gene94674 | 0.815939 | 7.15E-52 | -1.69E-46 |
| MS.gene052890 | MS.gene94674 | 0.809981 | 1.45E-50 | -1.69E-46 |
| MS.gene053228 | MS.gene94674 | 0.82066 | 6.10E-53 | -1.69E-46 |
| MS.gene053272 | MS.gene94674 | -0.840223 | 9.89E-58 | -1.69E-46 |
| MS.gene053298 | MS.gene94674 | 0.84315 | 1.67E-58 | -1.69E-46 |
| MS.gene053456 | MS.gene94674 | 0.809654 | 1.70E-50 | -1.69E-46 |
| MS.gene05350 | MS.gene94674 | 0.801281 | 9.69E-49 | -1.69E-46 |
| MS.gene053564 | MS.gene94674 | 0.801923 | 7.16E-49 | -1.69E-46 |
| MS.gene053726 | MS.gene94674 | 0.820978 | 5.15E-53 | -1.69E-46 |
| MS.gene053786 | MS.gene94674 | 0.803474 | 3.43E-49 | -1.69E-46 |
| MS.gene053879 | MS.gene94674 | 0.803503 | 3.38E-49 | -1.69E-46 |
| MS.gene053931 | MS.gene94674 | 0.82156 | 3.78E-53 | -1.69E-46 |
| MS.gene053963 | MS.gene94674 | 0.829746 | 4.33E-55 | -1.69E-46 |
| MS.gene054142 | MS.gene94674 | 0.803055 | 4.18E-49 | -1.69E-46 |
| MS.gene054178 | MS.gene94674 | 0.841755 | 3.92E-58 | -1.69E-46 |
| MS.gene054222 | MS.gene94674 | 0.825047 | 5.80E-54 | -1.69E-46 |
| MS.gene05423 | MS.gene94674 | 0.837568 | 4.81E-57 | -1.69E-46 |
| MS.gene054479 | MS.gene94674 | 0.826004 | 3.44E-54 | -1.69E-46 |
| MS.gene054489 | MS.gene94674 | 0.818166 | 2.26E-52 | -1.69E-46 |
| MS.gene05458 | MS.gene94674 | 0.83453 | 2.84E-56 | -1.69E-46 |
| MS.gene054641 | MS.gene94674 | 0.805897 | 1.07E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene94674 | 0.832585 | 8.69E-56 | -1.69E-46 |
| MS.gene05469 | MS.gene94674 | 0.807235 | 5.58E-50 | -1.69E-46 |
| MS.gene054772 | MS.gene94674 | 0.814954 | 1.18E-51 | -1.69E-46 |
| MS.gene054892 | MS.gene94674 | 0.867192 | 1.64E-65 | -1.69E-46 |
| MS.gene054893 | MS.gene94674 | 0.871986 | 4.50E-67 | -1.69E-46 |
| MS.gene054957 | MS.gene94674 | 0.815061 | 1.12E-51 | -1.69E-46 |
| MS.gene055150 | MS.gene94674 | 0.825808 | 3.83E-54 | -1.69E-46 |
| MS.gene055229 | MS.gene94674 | 0.808877 | 2.50E-50 | -1.69E-46 |
| MS.gene055386 | MS.gene94674 | 0.820833 | 5.57E-53 | -1.69E-46 |
| MS.gene05539 | MS.gene94674 | 0.813084 | 3.06E-51 | -1.69E-46 |
| MS.gene055513 | MS.gene94674 | 0.837992 | 3.75E-57 | -1.69E-46 |
| MS.gene05552 | MS.gene94674 | 0.805314 | 1.42E-49 | -1.69E-46 |
| MS.gene055724 | MS.gene94674 | 0.812112 | 4.99E-51 | -1.69E-46 |
| MS.gene055801 | MS.gene94674 | 0.819886 | 9.18E-53 | -1.69E-46 |
| MS.gene055841 | MS.gene94674 | 0.815731 | 7.96E-52 | -1.69E-46 |
| MS.gene055864 | MS.gene94674 | 0.818082 | 2.36E-52 | -1.69E-46 |
| MS.gene056045 | MS.gene94674 | 0.813288 | 2.76E-51 | -1.69E-46 |
| MS.gene056082 | MS.gene94674 | 0.848528 | 5.79E-60 | -1.69E-46 |
| MS.gene056174 | MS.gene94674 | 0.809245 | 2.08E-50 | -1.69E-46 |
| MS.gene056363 | MS.gene94674 | 0.800182 | 1.62E-48 | -1.69E-46 |
| MS.gene056677 | MS.gene94674 | 0.813741 | 2.19E-51 | -1.69E-46 |
| MS.gene056699 | MS.gene94674 | 0.848529 | 5.78E-60 | -1.69E-46 |
| MS.gene056776 | MS.gene94674 | 0.839847 | 1.24E-57 | -1.69E-46 |
| MS.gene056779 | MS.gene94674 | 0.809233 | 2.09E-50 | -1.69E-46 |
| MS.gene056825 | MS.gene94674 | 0.803981 | 2.69E-49 | -1.69E-46 |
| MS.gene056834 | MS.gene94674 | 0.813563 | 2.40E-51 | -1.69E-46 |
| MS.gene05686 | MS.gene94674 | 0.844568 | 6.98E-59 | -1.69E-46 |
| MS.gene05690 | MS.gene94674 | 0.83414 | 3.56E-56 | -1.69E-46 |
| MS.gene056973 | MS.gene94674 | 0.817853 | 2.66E-52 | -1.69E-46 |
| MS.gene056986 | MS.gene94674 | 0.811139 | 8.13E-51 | -1.69E-46 |
| MS.gene057034 | MS.gene94674 | 0.810907 | 9.13E-51 | -1.69E-46 |
| MS.gene057102 | MS.gene94674 | 0.867336 | 1.48E-65 | -1.69E-46 |
| MS.gene057155 | MS.gene94674 | 0.835901 | 1.28E-56 | -1.69E-46 |
| MS.gene057174 | MS.gene94674 | 0.802673 | 5.02E-49 | -1.69E-46 |
| MS.gene057208 | MS.gene94674 | 0.854832 | 9.46E-62 | -1.69E-46 |
| MS.gene057371 | MS.gene94674 | 0.834996 | 2.17E-56 | -1.69E-46 |
| MS.gene057611 | MS.gene94674 | 0.817063 | 4.01E-52 | -1.69E-46 |
| MS.gene057622 | MS.gene94674 | 0.831654 | 1.48E-55 | -1.69E-46 |
| MS.gene057890 | MS.gene94674 | -0.821238 | 4.49E-53 | -1.69E-46 |
| MS.gene057893 | MS.gene94674 | -0.815581 | 8.59E-52 | -1.69E-46 |
| MS.gene057919 | MS.gene94674 | 0.817286 | 3.57E-52 | -1.69E-46 |
| MS.gene058081 | MS.gene94674 | 0.809946 | 1.47E-50 | -1.69E-46 |
| MS.gene058095 | MS.gene94674 | 0.823367 | 1.44E-53 | -1.69E-46 |
| MS.gene058159 | MS.gene94674 | 0.85739 | 1.69E-62 | -1.69E-46 |
| MS.gene058279 | MS.gene94674 | 0.835394 | 1.72E-56 | -1.69E-46 |
| MS.gene058429 | MS.gene94674 | 0.839208 | 1.82E-57 | -1.69E-46 |
| MS.gene058592 | MS.gene94674 | 0.829278 | 5.63E-55 | -1.69E-46 |
| MS.gene058781 | MS.gene94674 | 0.844557 | 7.02E-59 | -1.69E-46 |
| MS.gene058783 | MS.gene94674 | 0.827766 | 1.31E-54 | -1.69E-46 |
| MS.gene05878 | MS.gene94674 | 0.821417 | 4.08E-53 | -1.69E-46 |
| MS.gene05897 | MS.gene94674 | 0.809503 | 1.83E-50 | -1.69E-46 |
| MS.gene059146 | MS.gene94674 | 0.849626 | 2.87E-60 | -1.69E-46 |
| MS.gene059170 | MS.gene94674 | 0.857169 | 1.96E-62 | -1.69E-46 |
| MS.gene05952 | MS.gene94674 | 0.874288 | 7.59E-68 | -1.69E-46 |
| MS.gene05956 | MS.gene94674 | 0.808363 | 3.21E-50 | -1.69E-46 |
| MS.gene05985 | MS.gene94674 | -0.867684 | 1.14E-65 | -1.69E-46 |
| MS.gene059901 | MS.gene94674 | -0.822837 | 1.91E-53 | -1.69E-46 |
| MS.gene06011 | MS.gene94674 | 0.801632 | 8.22E-49 | -1.69E-46 |
| MS.gene060244 | MS.gene94674 | 0.819164 | 1.34E-52 | -1.69E-46 |
| MS.gene060257 | MS.gene94674 | 0.800918 | 1.15E-48 | -1.69E-46 |
| MS.gene060259 | MS.gene94674 | 0.873034 | 2.01E-67 | -1.69E-46 |
| MS.gene060365 | MS.gene94674 | 0.834067 | 3.71E-56 | -1.69E-46 |
| MS.gene060412 | MS.gene94674 | 0.82161 | 3.68E-53 | -1.69E-46 |
| MS.gene060413 | MS.gene94674 | 0.803003 | 4.29E-49 | -1.69E-46 |
| MS.gene060435 | MS.gene94674 | 0.824346 | 8.48E-54 | -1.69E-46 |
| MS.gene060520 | MS.gene94674 | 0.803181 | 3.94E-49 | -1.69E-46 |
| MS.gene060522 | MS.gene94674 | 0.825352 | 4.91E-54 | -1.69E-46 |
| MS.gene060527 | MS.gene94674 | 0.830599 | 2.68E-55 | -1.69E-46 |
| MS.gene060777 | MS.gene94674 | 0.828287 | 9.78E-55 | -1.69E-46 |
| MS.gene060785 | MS.gene94674 | 0.805824 | 1.11E-49 | -1.69E-46 |
| MS.gene060932 | MS.gene94674 | 0.866302 | 3.15E-65 | -1.69E-46 |
| MS.gene060933 | MS.gene94674 | 0.837193 | 6.01E-57 | -1.69E-46 |
| MS.gene060935 | MS.gene94674 | 0.857481 | 1.59E-62 | -1.69E-46 |
| MS.gene060937 | MS.gene94674 | 0.872992 | 2.08E-67 | -1.69E-46 |
| MS.gene061100 | MS.gene94674 | 0.866002 | 3.92E-65 | -1.69E-46 |
| MS.gene061160 | MS.gene94674 | 0.846099 | 2.69E-59 | -1.69E-46 |
| MS.gene061188 | MS.gene94674 | 0.867123 | 1.73E-65 | -1.69E-46 |
| MS.gene06121 | MS.gene94674 | 0.803356 | 3.62E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94674.t1 | MTR_7g068770 | 97.727 | 176 | 3 | 1 | 1 | 175 | 118 | 293 | 3.83e-119 | 338 |
| MS.gene94674.t1 | MTR_4g015680 | 71.676 | 173 | 49 | 0 | 1 | 173 | 107 | 279 | 7.87e-61 | 190 |
| MS.gene94674.t1 | MTR_5g026540 | 42.473 | 186 | 87 | 6 | 8 | 174 | 117 | 301 | 1.97e-21 | 89.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94674.t1 | AT5G01380 | 47.959 | 196 | 78 | 3 | 2 | 173 | 118 | 313 | 7.37e-32 | 117 |
| MS.gene94674.t1 | AT2G38250 | 52.151 | 186 | 70 | 3 | 1 | 173 | 107 | 286 | 8.77e-27 | 102 |
Find 43 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTTGAAGCTCATTGTAAAA+TGG | 0.335464 | 7.3:+36456922 | None:intergenic |
| TGAGAGGAGATTGAAGGAGA+TGG | 0.420471 | 7.3:-36456611 | MS.gene94674:CDS |
| TGGAACAAAGATGGAGAGAA+AGG | 0.438127 | 7.3:-36456543 | MS.gene94674:CDS |
| GAAGGATTAGAGAGGAAGCT+AGG | 0.463838 | 7.3:-36456510 | MS.gene94674:CDS |
| GGAGATTGAAGGAGATGGAG+TGG | 0.466184 | 7.3:-36456606 | MS.gene94674:CDS |
| AGATTCTTGAGGAGTTTATG+AGG | 0.475642 | 7.3:-36456687 | MS.gene94674:CDS |
| TCAATGGATGGAAGCATTTG+AGG | 0.478413 | 7.3:-36456644 | MS.gene94674:CDS |
| TCTGAGGATGAAGAAGAGTT+AGG | 0.481694 | 7.3:-36456820 | MS.gene94674:CDS |
| AAGGAGGGTCAAAGAAGAAA+GGG | 0.488338 | 7.3:-36456855 | MS.gene94674:CDS |
| GATGGAGTGGAGACAAAGAA+TGG | 0.488486 | 7.3:-36456593 | MS.gene94674:CDS |
| GGAGAATGAGAGGAGATTGA+AGG | 0.494385 | 7.3:-36456617 | MS.gene94674:CDS |
| AAATGCAAATGGAAGCTCAA+TGG | 0.497271 | 7.3:-36456660 | MS.gene94674:CDS |
| TATGAGGCAACAAATGCAAA+TGG | 0.498234 | 7.3:-36456671 | MS.gene94674:CDS |
| GGTTAATGATGGAACAAAGA+TGG | 0.500019 | 7.3:-36456552 | MS.gene94674:CDS |
| GTTAGGCAATGAAGAAAGTG+AGG | 0.500463 | 7.3:-36456803 | MS.gene94674:CDS |
| GAAGGAGGGTCAAAGAAGAA+AGG | 0.513151 | 7.3:-36456856 | MS.gene94674:CDS |
| GAGACAAAGAATGGAAGCAT+TGG | 0.518130 | 7.3:-36456584 | MS.gene94674:CDS |
| AAAGGTAAAATGATAATTGG+TGG | 0.519046 | 7.3:-36456745 | MS.gene94674:CDS |
| CAAGGATGCAAAGAATGCTA+TGG | 0.520600 | 7.3:-36456888 | MS.gene94674:CDS |
| GAGAAAGGGAAGAACAAAGA+AGG | 0.520747 | 7.3:-36456528 | MS.gene94674:CDS |
| GGAACAAAGATGGAGAGAAA+GGG | 0.522554 | 7.3:-36456542 | MS.gene94674:CDS |
| TAGTGGTAGCAACAATTTGA+AGG | 0.523300 | 7.3:-36456710 | MS.gene94674:CDS |
| GGTGGAGGAAATGATAATAG+TGG | 0.531218 | 7.3:-36456727 | MS.gene94674:CDS |
| TGGAAGCATTGGAGAATGAG+AGG | 0.532379 | 7.3:-36456573 | MS.gene94674:CDS |
| GGAGAATGAGAGGTTAATGA+TGG | 0.535043 | 7.3:-36456563 | MS.gene94674:CDS |
| CAATTTGAAGGAGATTCTTG+AGG | 0.551112 | 7.3:-36456698 | MS.gene94674:CDS |
| GGTAAAATGATAATTGGTGG+AGG | 0.559022 | 7.3:-36456742 | MS.gene94674:CDS |
| AAATGGAAATTGTTGTCTCA+TGG | 0.560448 | 7.3:+36456939 | None:intergenic |
| AAGGATTAGAGAGGAAGCTA+GGG | 0.562239 | 7.3:-36456509 | MS.gene94674:CDS |
| ATGCTATGGGCTGAAGCAGA+AGG | 0.573045 | 7.3:-36456874 | MS.gene94674:CDS |
| GGATGGAAGCATTTGAGGCA+AGG | 0.573712 | 7.3:-36456639 | MS.gene94674:CDS |
| TTGAGGCAAGGGAGAATGAG+AGG | 0.584023 | 7.3:-36456627 | MS.gene94674:CDS |
| GCAAATGGAAGCTCAATGGA+TGG | 0.584747 | 7.3:-36456656 | MS.gene94674:CDS |
| GATGGAAGCATTTGAGGCAA+GGG | 0.587229 | 7.3:-36456638 | MS.gene94674:CDS |
| AGAAAGTGAGGGAGATCACA+AGG | 0.594638 | 7.3:-36456791 | MS.gene94674:CDS |
| TTAGGCAATGAAGAAAGTGA+GGG | 0.597635 | 7.3:-36456802 | MS.gene94674:CDS |
| CTATGGGCTGAAGCAGAAGG+AGG | 0.599474 | 7.3:-36456871 | MS.gene94674:CDS |
| AGAACAAAGAAGGATTAGAG+AGG | 0.604574 | 7.3:-36456518 | MS.gene94674:CDS |
| AGGGGTGCATGTATCTTCTG+AGG | 0.631436 | 7.3:-36456836 | MS.gene94674:CDS |
| AAGGATGCAAAGAATGCTAT+GGG | 0.642173 | 7.3:-36456887 | MS.gene94674:CDS |
| AGGAGGGTCAAAGAAGAAAG+GGG | 0.670389 | 7.3:-36456854 | MS.gene94674:CDS |
| TATGGGCTGAAGCAGAAGGA+GGG | 0.673793 | 7.3:-36456870 | MS.gene94674:CDS |
| GAAAGTGAGGGAGATCACAA+GGG | 0.730407 | 7.3:-36456790 | MS.gene94674:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAGGTAAAATGATAAT+TGG | - | chr7.3:36456651-36456670 | MS.gene94674:CDS | 15.0% |
| !! | ATTAAAAAGAAGAAGAAAAA+AGG | - | chr7.3:36456636-36456655 | MS.gene94674:CDS | 15.0% |
| ! | AAAGGTAAAATGATAATTGG+TGG | - | chr7.3:36456654-36456673 | MS.gene94674:CDS | 25.0% |
| AAATGGAAATTGTTGTCTCA+TGG | + | chr7.3:36456463-36456482 | None:intergenic | 30.0% | |
| !!! | TTCAAGCTATTTTTAGTGCA+AGG | - | chr7.3:36456493-36456512 | MS.gene94674:CDS | 30.0% |
| AAATGCAAATGGAAGCTCAA+TGG | - | chr7.3:36456739-36456758 | MS.gene94674:CDS | 35.0% | |
| AAGGATGCAAAGAATGCTAT+GGG | - | chr7.3:36456512-36456531 | MS.gene94674:CDS | 35.0% | |
| AGAACAAAGAAGGATTAGAG+AGG | - | chr7.3:36456881-36456900 | MS.gene94674:CDS | 35.0% | |
| AGATTCTTGAGGAGTTTATG+AGG | - | chr7.3:36456712-36456731 | MS.gene94674:CDS | 35.0% | |
| CAATTTGAAGGAGATTCTTG+AGG | - | chr7.3:36456701-36456720 | MS.gene94674:CDS | 35.0% | |
| GCTTGAAGCTCATTGTAAAA+TGG | + | chr7.3:36456480-36456499 | None:intergenic | 35.0% | |
| GGTAAAATGATAATTGGTGG+AGG | - | chr7.3:36456657-36456676 | MS.gene94674:CDS | 35.0% | |
| TAGTGGTAGCAACAATTTGA+AGG | - | chr7.3:36456689-36456708 | MS.gene94674:CDS | 35.0% | |
| TATGAGGCAACAAATGCAAA+TGG | - | chr7.3:36456728-36456747 | MS.gene94674:CDS | 35.0% | |
| TTAGGCAATGAAGAAAGTGA+GGG | - | chr7.3:36456597-36456616 | MS.gene94674:CDS | 35.0% | |
| ! | GGTTAATGATGGAACAAAGA+TGG | - | chr7.3:36456847-36456866 | MS.gene94674:CDS | 35.0% |
| AAGGAGGGTCAAAGAAGAAA+GGG | - | chr7.3:36456544-36456563 | MS.gene94674:CDS | 40.0% | |
| AAGGATTAGAGAGGAAGCTA+GGG | - | chr7.3:36456890-36456909 | MS.gene94674:CDS | 40.0% | |
| CAAGGATGCAAAGAATGCTA+TGG | - | chr7.3:36456511-36456530 | MS.gene94674:CDS | 40.0% | |
| GAGAAAGGGAAGAACAAAGA+AGG | - | chr7.3:36456871-36456890 | MS.gene94674:CDS | 40.0% | |
| GGAACAAAGATGGAGAGAAA+GGG | - | chr7.3:36456857-36456876 | MS.gene94674:CDS | 40.0% | |
| GGAGAATGAGAGGTTAATGA+TGG | - | chr7.3:36456836-36456855 | MS.gene94674:CDS | 40.0% | |
| GGTGGAGGAAATGATAATAG+TGG | - | chr7.3:36456672-36456691 | MS.gene94674:CDS | 40.0% | |
| GTTAGGCAATGAAGAAAGTG+AGG | - | chr7.3:36456596-36456615 | MS.gene94674:CDS | 40.0% | |
| TCTGAGGATGAAGAAGAGTT+AGG | - | chr7.3:36456579-36456598 | MS.gene94674:CDS | 40.0% | |
| TGGAACAAAGATGGAGAGAA+AGG | - | chr7.3:36456856-36456875 | MS.gene94674:CDS | 40.0% | |
| ! | GAGACAAAGAATGGAAGCAT+TGG | - | chr7.3:36456815-36456834 | MS.gene94674:CDS | 40.0% |
| !! | TCAATGGATGGAAGCATTTG+AGG | - | chr7.3:36456755-36456774 | MS.gene94674:CDS | 40.0% |
| AGAAAGTGAGGGAGATCACA+AGG | - | chr7.3:36456608-36456627 | MS.gene94674:CDS | 45.0% | |
| AGGAGGGTCAAAGAAGAAAG+GGG | - | chr7.3:36456545-36456564 | MS.gene94674:CDS | 45.0% | |
| GAAAGTGAGGGAGATCACAA+GGG | - | chr7.3:36456609-36456628 | MS.gene94674:CDS | 45.0% | |
| GAAGGAGGGTCAAAGAAGAA+AGG | - | chr7.3:36456543-36456562 | MS.gene94674:CDS | 45.0% | |
| GAAGGATTAGAGAGGAAGCT+AGG | - | chr7.3:36456889-36456908 | MS.gene94674:CDS | 45.0% | |
| GATGGAGTGGAGACAAAGAA+TGG | - | chr7.3:36456806-36456825 | MS.gene94674:CDS | 45.0% | |
| GCAAATGGAAGCTCAATGGA+TGG | - | chr7.3:36456743-36456762 | MS.gene94674:CDS | 45.0% | |
| GGAGAATGAGAGGAGATTGA+AGG | - | chr7.3:36456782-36456801 | MS.gene94674:CDS | 45.0% | |
| TGAGAGGAGATTGAAGGAGA+TGG | - | chr7.3:36456788-36456807 | MS.gene94674:CDS | 45.0% | |
| !! | GATGGAAGCATTTGAGGCAA+GGG | - | chr7.3:36456761-36456780 | MS.gene94674:CDS | 45.0% |
| !! | TGGAAGCATTGGAGAATGAG+AGG | - | chr7.3:36456826-36456845 | MS.gene94674:CDS | 45.0% |
| GGAGATTGAAGGAGATGGAG+TGG | - | chr7.3:36456793-36456812 | MS.gene94674:CDS | 50.0% | |
| TTGAGGCAAGGGAGAATGAG+AGG | - | chr7.3:36456772-36456791 | MS.gene94674:CDS | 50.0% | |
| ! | AGGGGTGCATGTATCTTCTG+AGG | - | chr7.3:36456563-36456582 | MS.gene94674:CDS | 50.0% |
| ! | ATGCTATGGGCTGAAGCAGA+AGG | - | chr7.3:36456525-36456544 | MS.gene94674:CDS | 50.0% |
| ! | TATGGGCTGAAGCAGAAGGA+GGG | - | chr7.3:36456529-36456548 | MS.gene94674:CDS | 50.0% |
| !! | GGATGGAAGCATTTGAGGCA+AGG | - | chr7.3:36456760-36456779 | MS.gene94674:CDS | 50.0% |
| ! | CTATGGGCTGAAGCAGAAGG+AGG | - | chr7.3:36456528-36456547 | MS.gene94674:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 36456447 | 36456974 | 36456447 | ID=MS.gene94674 |
| chr7.3 | mRNA | 36456447 | 36456974 | 36456447 | ID=MS.gene94674.t1;Parent=MS.gene94674 |
| chr7.3 | exon | 36456447 | 36456974 | 36456447 | ID=MS.gene94674.t1.exon1;Parent=MS.gene94674.t1 |
| chr7.3 | CDS | 36456447 | 36456974 | 36456447 | ID=cds.MS.gene94674.t1;Parent=MS.gene94674.t1 |
| Gene Sequence |
| Protein sequence |
