Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88147.t1 | AES87683.2 | 97 | 233 | 7 | 0 | 1 | 233 | 199 | 431 | 3.50E-126 | 461.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88147.t1 | G7JN46 | 97.0 | 233 | 7 | 0 | 1 | 233 | 199 | 431 | 2.5e-126 | 461.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene88147 | 0.834255 | 3.33E-56 | -1.69E-46 |
| MS.gene04919 | MS.gene88147 | 0.828898 | 6.96E-55 | -1.69E-46 |
| MS.gene04920 | MS.gene88147 | 0.846497 | 2.09E-59 | -1.69E-46 |
| MS.gene049254 | MS.gene88147 | 0.80056 | 1.36E-48 | -1.69E-46 |
| MS.gene049283 | MS.gene88147 | 0.830361 | 3.07E-55 | -1.69E-46 |
| MS.gene049350 | MS.gene88147 | 0.82933 | 5.47E-55 | -1.69E-46 |
| MS.gene049499 | MS.gene88147 | 0.838911 | 2.17E-57 | -1.69E-46 |
| MS.gene049680 | MS.gene88147 | 0.813069 | 3.08E-51 | -1.69E-46 |
| MS.gene049807 | MS.gene88147 | 0.820655 | 6.12E-53 | -1.69E-46 |
| MS.gene049983 | MS.gene88147 | 0.824803 | 6.62E-54 | -1.69E-46 |
| MS.gene050300 | MS.gene88147 | -0.809578 | 1.77E-50 | -1.69E-46 |
| MS.gene050414 | MS.gene88147 | 0.84975 | 2.65E-60 | -1.69E-46 |
| MS.gene050646 | MS.gene88147 | 0.822685 | 2.08E-53 | -1.69E-46 |
| MS.gene050654 | MS.gene88147 | 0.807239 | 5.57E-50 | -1.69E-46 |
| MS.gene050752 | MS.gene88147 | 0.805083 | 1.58E-49 | -1.69E-46 |
| MS.gene050838 | MS.gene88147 | 0.815937 | 7.16E-52 | -1.69E-46 |
| MS.gene050945 | MS.gene88147 | 0.839004 | 2.05E-57 | -1.69E-46 |
| MS.gene050955 | MS.gene88147 | 0.809718 | 1.65E-50 | -1.69E-46 |
| MS.gene051003 | MS.gene88147 | 0.82979 | 4.23E-55 | -1.69E-46 |
| MS.gene051043 | MS.gene88147 | 0.814098 | 1.83E-51 | -1.69E-46 |
| MS.gene051234 | MS.gene88147 | 0.844936 | 5.55E-59 | -1.69E-46 |
| MS.gene051343 | MS.gene88147 | 0.834255 | 3.33E-56 | -1.69E-46 |
| MS.gene051597 | MS.gene88147 | 0.800863 | 1.18E-48 | -1.69E-46 |
| MS.gene051628 | MS.gene88147 | 0.803988 | 2.68E-49 | -1.69E-46 |
| MS.gene051699 | MS.gene88147 | 0.836055 | 1.17E-56 | -1.69E-46 |
| MS.gene051852 | MS.gene88147 | 0.821533 | 3.84E-53 | -1.69E-46 |
| MS.gene051902 | MS.gene88147 | 0.812499 | 4.11E-51 | -1.69E-46 |
| MS.gene052016 | MS.gene88147 | 0.812145 | 4.91E-51 | -1.69E-46 |
| MS.gene052072 | MS.gene88147 | 0.829092 | 6.25E-55 | -1.69E-46 |
| MS.gene052242 | MS.gene88147 | -0.807314 | 5.37E-50 | -1.69E-46 |
| MS.gene052296 | MS.gene88147 | 0.814646 | 1.39E-51 | -1.69E-46 |
| MS.gene052307 | MS.gene88147 | 0.825621 | 4.24E-54 | -1.69E-46 |
| MS.gene052335 | MS.gene88147 | 0.81364 | 2.31E-51 | -1.69E-46 |
| MS.gene052342 | MS.gene88147 | 0.823207 | 1.57E-53 | -1.69E-46 |
| MS.gene052463 | MS.gene88147 | 0.81982 | 9.50E-53 | -1.69E-46 |
| MS.gene052479 | MS.gene88147 | 0.807336 | 5.31E-50 | -1.69E-46 |
| MS.gene052516 | MS.gene88147 | 0.810976 | 8.82E-51 | -1.69E-46 |
| MS.gene052533 | MS.gene88147 | 0.817808 | 2.72E-52 | -1.69E-46 |
| MS.gene052793 | MS.gene88147 | 0.828512 | 8.63E-55 | -1.69E-46 |
| MS.gene053272 | MS.gene88147 | -0.82406 | 9.91E-54 | -1.69E-46 |
| MS.gene054004 | MS.gene88147 | 0.820464 | 6.76E-53 | -1.69E-46 |
| MS.gene054178 | MS.gene88147 | 0.835142 | 1.99E-56 | -1.69E-46 |
| MS.gene054222 | MS.gene88147 | 0.805851 | 1.09E-49 | -1.69E-46 |
| MS.gene054403 | MS.gene88147 | 0.809677 | 1.68E-50 | -1.69E-46 |
| MS.gene054709 | MS.gene88147 | 0.827427 | 1.57E-54 | -1.69E-46 |
| MS.gene054957 | MS.gene88147 | 0.800521 | 1.39E-48 | -1.69E-46 |
| MS.gene055513 | MS.gene88147 | 0.825733 | 3.99E-54 | -1.69E-46 |
| MS.gene055555 | MS.gene88147 | 0.800504 | 1.40E-48 | -1.69E-46 |
| MS.gene056082 | MS.gene88147 | 0.819459 | 1.15E-52 | -1.69E-46 |
| MS.gene056272 | MS.gene88147 | 0.804679 | 1.92E-49 | -1.69E-46 |
| MS.gene056308 | MS.gene88147 | 0.838705 | 2.45E-57 | -1.69E-46 |
| MS.gene056578 | MS.gene88147 | 0.819028 | 1.44E-52 | -1.69E-46 |
| MS.gene056677 | MS.gene88147 | 0.817803 | 2.73E-52 | -1.69E-46 |
| MS.gene056699 | MS.gene88147 | 0.807849 | 4.13E-50 | -1.69E-46 |
| MS.gene056776 | MS.gene88147 | 0.814972 | 1.17E-51 | -1.69E-46 |
| MS.gene057102 | MS.gene88147 | 0.861395 | 1.06E-63 | -1.69E-46 |
| MS.gene05781 | MS.gene88147 | 0.803576 | 3.26E-49 | -1.69E-46 |
| MS.gene057890 | MS.gene88147 | -0.827102 | 1.88E-54 | -1.69E-46 |
| MS.gene057893 | MS.gene88147 | -0.827606 | 1.43E-54 | -1.69E-46 |
| MS.gene057919 | MS.gene88147 | 0.818363 | 2.04E-52 | -1.69E-46 |
| MS.gene058008 | MS.gene88147 | 0.817594 | 3.04E-52 | -1.69E-46 |
| MS.gene058159 | MS.gene88147 | 0.823665 | 1.23E-53 | -1.69E-46 |
| MS.gene058279 | MS.gene88147 | 0.803076 | 4.14E-49 | -1.69E-46 |
| MS.gene058330 | MS.gene88147 | 0.826675 | 2.38E-54 | -1.69E-46 |
| MS.gene058781 | MS.gene88147 | 0.809384 | 1.94E-50 | -1.69E-46 |
| MS.gene059146 | MS.gene88147 | 0.835817 | 1.35E-56 | -1.69E-46 |
| MS.gene059170 | MS.gene88147 | 0.819318 | 1.24E-52 | -1.69E-46 |
| MS.gene059778 | MS.gene88147 | 0.807581 | 4.71E-50 | -1.69E-46 |
| MS.gene05985 | MS.gene88147 | -0.834982 | 2.19E-56 | -1.69E-46 |
| MS.gene060365 | MS.gene88147 | 0.817908 | 2.58E-52 | -1.69E-46 |
| MS.gene060932 | MS.gene88147 | 0.803275 | 3.77E-49 | -1.69E-46 |
| MS.gene060933 | MS.gene88147 | 0.80168 | 8.03E-49 | -1.69E-46 |
| MS.gene060935 | MS.gene88147 | 0.831879 | 1.30E-55 | -1.69E-46 |
| MS.gene060937 | MS.gene88147 | 0.842601 | 2.34E-58 | -1.69E-46 |
| MS.gene061100 | MS.gene88147 | 0.886209 | 4.17E-72 | -1.69E-46 |
| MS.gene061160 | MS.gene88147 | 0.847425 | 1.17E-59 | -1.69E-46 |
| MS.gene061243 | MS.gene88147 | 0.80765 | 4.56E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88147.t1 | MTR_4g032190 | 95.378 | 238 | 11 | 0 | 1 | 238 | 199 | 436 | 5.07e-169 | 474 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88147.t1 | AT5G42670 | 37.168 | 226 | 97 | 4 | 1 | 226 | 98 | 278 | 1.15e-38 | 137 |
| MS.gene88147.t1 | AT5G52070 | 34.483 | 232 | 96 | 5 | 1 | 230 | 22 | 199 | 2.51e-36 | 129 |
| MS.gene88147.t1 | AT5G52070 | 34.483 | 232 | 96 | 5 | 1 | 230 | 25 | 202 | 2.78e-36 | 129 |
| MS.gene88147.t1 | AT5G52070 | 34.483 | 232 | 96 | 5 | 1 | 230 | 25 | 202 | 2.78e-36 | 129 |
| MS.gene88147.t1 | AT5G52070 | 34.483 | 232 | 96 | 5 | 1 | 230 | 25 | 202 | 2.78e-36 | 129 |
| MS.gene88147.t1 | AT5G52070 | 34.483 | 232 | 96 | 5 | 1 | 230 | 74 | 251 | 1.35e-35 | 128 |
| MS.gene88147.t1 | AT5G52070 | 34.483 | 232 | 96 | 5 | 1 | 230 | 74 | 251 | 1.35e-35 | 128 |
Find 70 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGAATGGAAGGTTGGGGATT+TGG | 0.234306 | 4.3:-69027376 | MS.gene88147:CDS |
| TATTTCAAGCTCACATATTA+TGG | 0.236755 | 4.3:-69022079 | MS.gene88147:CDS |
| CAAGGACCTGCGCCCTTCTT+TGG | 0.263417 | 4.3:-69025056 | MS.gene88147:CDS |
| AACTTGTAAATAAGATTAAA+TGG | 0.284890 | 4.3:-69021906 | MS.gene88147:CDS |
| TACAATGGAATCAGCAATTC+TGG | 0.286651 | 4.3:-69021938 | MS.gene88147:CDS |
| TTTGAGTGAGTTTATGCAAT+AGG | 0.319693 | 4.3:-69019208 | None:intergenic |
| CACACAGTCCAACCTTTCTC+TGG | 0.352617 | 4.3:+69025025 | None:intergenic |
| ATTTGGAAATGATAAAGTTC+AGG | 0.352956 | 4.3:-69027304 | MS.gene88147:intron |
| AAGTGTGAGGTGCCTTTATC+AGG | 0.355820 | 4.3:-69021868 | MS.gene88147:intron |
| GGGCGTTTGGCACCTGATAA+AGG | 0.369471 | 4.3:+69021856 | None:intergenic |
| GGAAAAGTGACTGAAGTATT+TGG | 0.372820 | 4.3:-69027321 | MS.gene88147:CDS |
| CAACTCTGTTGGAAACAGTA+TGG | 0.405352 | 4.3:-69022139 | MS.gene88147:CDS |
| CTGGAAGCTCTATCATTATC+TGG | 0.413751 | 4.3:-69019267 | MS.gene88147:CDS |
| ACCTGCGCCCTTCTTTGGAC+TGG | 0.433874 | 4.3:-69025051 | MS.gene88147:CDS |
| ATAATGAATCCAGCAAGTTC+AGG | 0.435565 | 4.3:-69023223 | MS.gene88147:intron |
| TTGCAAAATTTGCAGGAAGA+AGG | 0.438561 | 4.3:-69023265 | MS.gene88147:intron |
| TTGCTTCTCATCTTCTTAGC+AGG | 0.438736 | 4.3:+69021994 | None:intergenic |
| TTGATGACGAATGGAAGGTT+GGG | 0.451757 | 4.3:-69027383 | MS.gene88147:CDS |
| TATAGTGGTCATCCTTCCTC+TGG | 0.457587 | 4.3:-69019286 | MS.gene88147:intron |
| GGAATCAGCAATTCTGGACT+TGG | 0.460492 | 4.3:-69021932 | MS.gene88147:CDS |
| CAAAGAAGGGCGCAGGTCCT+TGG | 0.465031 | 4.3:+69025057 | None:intergenic |
| AGGTTGGGGATTTGGTAGAT+TGG | 0.470989 | 4.3:-69027368 | MS.gene88147:CDS |
| TCTGGGCTCCAGTCCAAAGA+AGG | 0.490355 | 4.3:+69025043 | None:intergenic |
| CTACCACCTCCGCTAGGTGA+AGG | 0.491762 | 4.3:-69025100 | MS.gene88147:CDS |
| ATCATTACATCGACTGTTTG+AGG | 0.496897 | 4.3:+69022026 | None:intergenic |
| GACCCTTCACCTAGCGGAGG+TGG | 0.508332 | 4.3:+69025097 | None:intergenic |
| AGAGAGCAACGCAACTCTGT+TGG | 0.511363 | 4.3:-69022150 | MS.gene88147:CDS |
| GTTGATGACGAATGGAAGGT+TGG | 0.511379 | 4.3:-69027384 | MS.gene88147:CDS |
| TTGGACTGGAGCCCAGAGAA+AGG | 0.515083 | 4.3:-69025037 | MS.gene88147:CDS |
| TCCAGTCCAAAGAAGGGCGC+AGG | 0.520174 | 4.3:+69025050 | None:intergenic |
| TCACTCAAAGGTATACCTTC+CGG | 0.526591 | 4.3:+69019222 | None:intergenic |
| GCGTTGCTCTCTCTTCTCCA+CGG | 0.530527 | 4.3:+69022161 | None:intergenic |
| TTCCATTGTATTCGAAGACA+TGG | 0.537313 | 4.3:+69021951 | None:intergenic |
| GCTGGATTCATTATCCGAGC+AGG | 0.538397 | 4.3:+69023232 | None:intergenic |
| GAGGAGGCTGGCAGTAAAAG+TGG | 0.541052 | 4.3:-69022111 | MS.gene88147:CDS |
| TGGAAACAGTATGGATATTG+AGG | 0.542898 | 4.3:-69022130 | MS.gene88147:CDS |
| TCTGATGGTTGCTATTGGTG+TGG | 0.546729 | 4.3:-69027342 | MS.gene88147:CDS |
| ACTGGAGCCCAGAGAAAGGT+TGG | 0.549135 | 4.3:-69025033 | MS.gene88147:CDS |
| TCACTTTCACGATAGATGGT+AGG | 0.553522 | 4.3:+69027447 | None:intergenic |
| TTCACGATAGATGGTAGGAA+AGG | 0.556485 | 4.3:+69027452 | None:intergenic |
| ATTTGCAGGAAGAAGGATGC+AGG | 0.565446 | 4.3:-69023258 | MS.gene88147:intron |
| TCACGATAGATGGTAGGAAA+GGG | 0.565643 | 4.3:+69027453 | None:intergenic |
| ATGATAGAGCTTCCAGAGGA+AGG | 0.567474 | 4.3:+69019274 | None:intergenic |
| TAGTTATTGTTGATGACGAA+TGG | 0.567724 | 4.3:-69027392 | MS.gene88147:CDS |
| TGATGACGAATGGAAGGTTG+GGG | 0.570750 | 4.3:-69027382 | MS.gene88147:CDS |
| TACCACCTCCGCTAGGTGAA+GGG | 0.571753 | 4.3:-69025099 | MS.gene88147:CDS |
| AATAACTATCACTTCTGAGA+AGG | 0.572635 | 4.3:+69027407 | None:intergenic |
| AAGGATGCAGGTGTCCTGCT+CGG | 0.582503 | 4.3:-69023246 | MS.gene88147:CDS |
| TGGAATTCAGACAAAGGCTA+AGG | 0.584533 | 4.3:+69021971 | None:intergenic |
| ACATGAAGATACAACGCTGC+CGG | 0.587475 | 4.3:-69019241 | MS.gene88147:CDS |
| TTCCATGTCTTCGAATACAA+TGG | 0.593562 | 4.3:-69021953 | MS.gene88147:CDS |
| ACAGATGGATCAGTGAGCAT+TGG | 0.594650 | 4.3:-69022201 | MS.gene88147:intron |
| ACACAGTCCAACCTTTCTCT+GGG | 0.595931 | 4.3:+69025026 | None:intergenic |
| CTGGGCTCCAGTCCAAAGAA+GGG | 0.597405 | 4.3:+69025044 | None:intergenic |
| TATTGCATAAACTCACTCAA+AGG | 0.598127 | 4.3:+69019210 | None:intergenic |
| TATTGTTGATGACGAATGGA+AGG | 0.603504 | 4.3:-69027388 | MS.gene88147:CDS |
| GATTTGCTACCACCTCCGCT+AGG | 0.605782 | 4.3:-69025106 | MS.gene88147:CDS |
| ATCGTATGAAGCTTTAACCA+AGG | 0.606477 | 4.3:-69025074 | MS.gene88147:CDS |
| AGTATGGATATTGAGGAGGC+TGG | 0.608407 | 4.3:-69022123 | MS.gene88147:CDS |
| TTGGACTGTGTGTATGCCTA+CGG | 0.613510 | 4.3:-69025014 | MS.gene88147:intron |
| CGATAGATGGTAGGAAAGGG+AGG | 0.634082 | 4.3:+69027456 | None:intergenic |
| GAAGATACAACGCTGCCGGA+AGG | 0.637030 | 4.3:-69019237 | MS.gene88147:CDS |
| TACGATGACCCTTCACCTAG+CGG | 0.652144 | 4.3:+69025091 | None:intergenic |
| AAGACATGGAATTCAGACAA+AGG | 0.670613 | 4.3:+69021965 | None:intergenic |
| AAACAGTATGGATATTGAGG+AGG | 0.683902 | 4.3:-69022127 | MS.gene88147:CDS |
| GAGGCTGGCAGTAAAAGTGG+AGG | 0.688773 | 4.3:-69022108 | MS.gene88147:CDS |
| AAGAGCTGTGTTGAAGTGTG+AGG | 0.706586 | 4.3:-69021881 | MS.gene88147:CDS |
| GATGACCCTTCACCTAGCGG+AGG | 0.713753 | 4.3:+69025094 | None:intergenic |
| GATAATGATAGAGCTTCCAG+AGG | 0.713772 | 4.3:+69019270 | None:intergenic |
| TCAGTCTTCATACAGAACCG+TGG | 0.722004 | 4.3:-69022178 | MS.gene88147:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 69019209 | 69027487 | 69019209 | ID=MS.gene88147 |
| chr4.3 | mRNA | 69019209 | 69027487 | 69019209 | ID=MS.gene88147.t1;Parent=MS.gene88147 |
| chr4.3 | exon | 69027305 | 69027487 | 69027305 | ID=MS.gene88147.t1.exon1;Parent=MS.gene88147.t1 |
| chr4.3 | CDS | 69027305 | 69027487 | 69027305 | ID=cds.MS.gene88147.t1;Parent=MS.gene88147.t1 |
| chr4.3 | exon | 69025015 | 69025131 | 69025015 | ID=MS.gene88147.t1.exon2;Parent=MS.gene88147.t1 |
| chr4.3 | CDS | 69025015 | 69025131 | 69025015 | ID=cds.MS.gene88147.t1;Parent=MS.gene88147.t1 |
| chr4.3 | exon | 69023224 | 69023272 | 69023224 | ID=MS.gene88147.t1.exon3;Parent=MS.gene88147.t1 |
| chr4.3 | CDS | 69023224 | 69023272 | 69023224 | ID=cds.MS.gene88147.t1;Parent=MS.gene88147.t1 |
| chr4.3 | exon | 69021869 | 69022219 | 69021869 | ID=MS.gene88147.t1.exon4;Parent=MS.gene88147.t1 |
| chr4.3 | CDS | 69021869 | 69022219 | 69021869 | ID=cds.MS.gene88147.t1;Parent=MS.gene88147.t1 |
| chr4.3 | exon | 69019209 | 69019303 | 69019209 | ID=MS.gene88147.t1.exon5;Parent=MS.gene88147.t1 |
| chr4.3 | CDS | 69019209 | 69019303 | 69019209 | ID=cds.MS.gene88147.t1;Parent=MS.gene88147.t1 |
| Gene Sequence |
| Protein sequence |
