Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57576.t1 | XP_013458468.1 | 92.9 | 126 | 2 | 3 | 1 | 122 | 1 | 123 | 3.70E-54 | 220.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57576.t1 | I3SBP3 | 92.9 | 126 | 2 | 3 | 1 | 122 | 1 | 123 | 2.7e-54 | 220.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04920 | MS.gene57576 | 0.803383 | 3.58E-49 | -1.69E-46 |
| MS.gene049254 | MS.gene57576 | 0.811766 | 5.94E-51 | -1.69E-46 |
| MS.gene049283 | MS.gene57576 | 0.827721 | 1.34E-54 | -1.69E-46 |
| MS.gene049350 | MS.gene57576 | 0.800395 | 1.47E-48 | -1.69E-46 |
| MS.gene049499 | MS.gene57576 | 0.801031 | 1.09E-48 | -1.69E-46 |
| MS.gene049711 | MS.gene57576 | 0.812829 | 3.48E-51 | -1.69E-46 |
| MS.gene049807 | MS.gene57576 | 0.827985 | 1.16E-54 | -1.69E-46 |
| MS.gene04998 | MS.gene57576 | 0.824819 | 6.56E-54 | -1.69E-46 |
| MS.gene050253 | MS.gene57576 | 0.812776 | 3.58E-51 | -1.69E-46 |
| MS.gene050300 | MS.gene57576 | -0.808098 | 3.66E-50 | -1.69E-46 |
| MS.gene050414 | MS.gene57576 | 0.854444 | 1.23E-61 | -1.69E-46 |
| MS.gene050556 | MS.gene57576 | 0.823079 | 1.68E-53 | -1.69E-46 |
| MS.gene050654 | MS.gene57576 | 0.806797 | 6.91E-50 | -1.69E-46 |
| MS.gene050945 | MS.gene57576 | 0.800885 | 1.17E-48 | -1.69E-46 |
| MS.gene051029 | MS.gene57576 | 0.822147 | 2.77E-53 | -1.69E-46 |
| MS.gene051043 | MS.gene57576 | 0.839079 | 1.96E-57 | -1.69E-46 |
| MS.gene051201 | MS.gene57576 | 0.811035 | 8.56E-51 | -1.69E-46 |
| MS.gene051234 | MS.gene57576 | 0.809355 | 1.97E-50 | -1.69E-46 |
| MS.gene051279 | MS.gene57576 | 0.829876 | 4.03E-55 | -1.69E-46 |
| MS.gene051343 | MS.gene57576 | 0.803315 | 3.70E-49 | -1.69E-46 |
| MS.gene051676 | MS.gene57576 | 0.823535 | 1.31E-53 | -1.69E-46 |
| MS.gene051852 | MS.gene57576 | 0.800734 | 1.25E-48 | -1.69E-46 |
| MS.gene051902 | MS.gene57576 | 0.836551 | 8.76E-57 | -1.69E-46 |
| MS.gene052016 | MS.gene57576 | 0.870108 | 1.87E-66 | -1.69E-46 |
| MS.gene052307 | MS.gene57576 | 0.801351 | 9.38E-49 | -1.69E-46 |
| MS.gene052335 | MS.gene57576 | 0.801456 | 8.93E-49 | -1.69E-46 |
| MS.gene052463 | MS.gene57576 | 0.846727 | 1.81E-59 | -1.69E-46 |
| MS.gene052493 | MS.gene57576 | 0.803905 | 2.79E-49 | -1.69E-46 |
| MS.gene052533 | MS.gene57576 | 0.81872 | 1.69E-52 | -1.69E-46 |
| MS.gene052582 | MS.gene57576 | 0.80328 | 3.76E-49 | -1.69E-46 |
| MS.gene052584 | MS.gene57576 | 0.80773 | 4.38E-50 | -1.69E-46 |
| MS.gene052673 | MS.gene57576 | -0.803946 | 2.74E-49 | -1.69E-46 |
| MS.gene053079 | MS.gene57576 | 0.831686 | 1.45E-55 | -1.69E-46 |
| MS.gene053298 | MS.gene57576 | 0.807798 | 4.24E-50 | -1.69E-46 |
| MS.gene053620 | MS.gene57576 | 0.810813 | 9.57E-51 | -1.69E-46 |
| MS.gene053786 | MS.gene57576 | 0.810837 | 9.45E-51 | -1.69E-46 |
| MS.gene054178 | MS.gene57576 | 0.874116 | 8.68E-68 | -1.69E-46 |
| MS.gene054222 | MS.gene57576 | 0.814273 | 1.68E-51 | -1.69E-46 |
| MS.gene05423 | MS.gene57576 | 0.813418 | 2.59E-51 | -1.69E-46 |
| MS.gene054576 | MS.gene57576 | 0.801265 | 9.77E-49 | -1.69E-46 |
| MS.gene054617 | MS.gene57576 | 0.801641 | 8.18E-49 | -1.69E-46 |
| MS.gene054772 | MS.gene57576 | 0.801239 | 9.89E-49 | -1.69E-46 |
| MS.gene054892 | MS.gene57576 | 0.832847 | 7.48E-56 | -1.69E-46 |
| MS.gene054893 | MS.gene57576 | 0.835512 | 1.61E-56 | -1.69E-46 |
| MS.gene055229 | MS.gene57576 | 0.824507 | 7.77E-54 | -1.69E-46 |
| MS.gene05539 | MS.gene57576 | 0.834895 | 2.30E-56 | -1.69E-46 |
| MS.gene055513 | MS.gene57576 | 0.86344 | 2.49E-64 | -1.69E-46 |
| MS.gene056045 | MS.gene57576 | 0.803766 | 2.98E-49 | -1.69E-46 |
| MS.gene056082 | MS.gene57576 | 0.830227 | 3.31E-55 | -1.69E-46 |
| MS.gene056308 | MS.gene57576 | 0.816646 | 4.97E-52 | -1.69E-46 |
| MS.gene056390 | MS.gene57576 | 0.81668 | 4.88E-52 | -1.69E-46 |
| MS.gene056677 | MS.gene57576 | 0.804594 | 2.00E-49 | -1.69E-46 |
| MS.gene057102 | MS.gene57576 | 0.848971 | 4.36E-60 | -1.69E-46 |
| MS.gene057919 | MS.gene57576 | 0.82394 | 1.06E-53 | -1.69E-46 |
| MS.gene058279 | MS.gene57576 | 0.805509 | 1.29E-49 | -1.69E-46 |
| MS.gene058330 | MS.gene57576 | 0.80254 | 5.34E-49 | -1.69E-46 |
| MS.gene058492 | MS.gene57576 | 0.820391 | 7.03E-53 | -1.69E-46 |
| MS.gene058681 | MS.gene57576 | 0.801528 | 8.63E-49 | -1.69E-46 |
| MS.gene058721 | MS.gene57576 | 0.801877 | 7.32E-49 | -1.69E-46 |
| MS.gene059170 | MS.gene57576 | 0.808475 | 3.04E-50 | -1.69E-46 |
| MS.gene059227 | MS.gene57576 | 0.806194 | 9.26E-50 | -1.69E-46 |
| MS.gene059774 | MS.gene57576 | 0.803505 | 3.38E-49 | -1.69E-46 |
| MS.gene05985 | MS.gene57576 | -0.835111 | 2.03E-56 | -1.69E-46 |
| MS.gene059965 | MS.gene57576 | 0.821196 | 4.59E-53 | -1.69E-46 |
| MS.gene060259 | MS.gene57576 | 0.801267 | 9.76E-49 | -1.69E-46 |
| MS.gene060365 | MS.gene57576 | 0.814291 | 1.66E-51 | -1.69E-46 |
| MS.gene060603 | MS.gene57576 | 0.80265 | 5.07E-49 | -1.69E-46 |
| MS.gene060697 | MS.gene57576 | 0.800443 | 1.44E-48 | -1.69E-46 |
| MS.gene060932 | MS.gene57576 | 0.824587 | 7.45E-54 | -1.69E-46 |
| MS.gene061100 | MS.gene57576 | 0.863373 | 2.61E-64 | -1.69E-46 |
| MS.gene061160 | MS.gene57576 | 0.802991 | 4.31E-49 | -1.69E-46 |
| MS.gene061188 | MS.gene57576 | 0.80584 | 1.10E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57576.t1 | MTR_4g125990 | 92.857 | 126 | 2 | 3 | 1 | 122 | 1 | 123 | 8.64e-78 | 225 |
| MS.gene57576.t1 | MTR_3g104640 | 55.200 | 125 | 38 | 4 | 1 | 122 | 1 | 110 | 1.84e-28 | 100 |
| MS.gene57576.t1 | MTR_2g099630 | 50.000 | 120 | 44 | 5 | 3 | 121 | 2 | 106 | 2.97e-28 | 99.8 |
| MS.gene57576.t1 | MTR_8g018320 | 52.137 | 117 | 40 | 4 | 3 | 118 | 2 | 103 | 2.71e-27 | 97.4 |
| MS.gene57576.t1 | MTR_8g018360 | 50.000 | 120 | 40 | 4 | 3 | 119 | 2 | 104 | 3.05e-26 | 97.1 |
| MS.gene57576.t1 | MTR_1g015810 | 49.206 | 126 | 48 | 6 | 1 | 120 | 1 | 116 | 1.82e-18 | 75.1 |
| MS.gene57576.t1 | MTR_7g109290 | 43.182 | 88 | 40 | 2 | 43 | 122 | 6 | 91 | 4.16e-18 | 73.6 |
| MS.gene57576.t1 | MTR_1g087730 | 51.923 | 52 | 25 | 0 | 71 | 122 | 55 | 106 | 2.72e-16 | 69.3 |
| MS.gene57576.t1 | MTR_1g098310 | 54.386 | 57 | 23 | 1 | 68 | 121 | 29 | 85 | 8.16e-12 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57576.t1 | AT2G19460 | 54.331 | 127 | 40 | 6 | 1 | 120 | 1 | 116 | 2.83e-34 | 115 |
| MS.gene57576.t1 | AT2G19460 | 54.331 | 127 | 40 | 6 | 1 | 120 | 53 | 168 | 5.19e-34 | 116 |
| MS.gene57576.t1 | AT5G11970 | 53.659 | 123 | 37 | 6 | 1 | 122 | 1 | 104 | 4.70e-31 | 107 |
| MS.gene57576.t1 | AT3G13910 | 46.392 | 97 | 46 | 2 | 20 | 113 | 4 | 97 | 3.81e-19 | 76.6 |
| MS.gene57576.t1 | AT3G13910 | 46.392 | 97 | 46 | 2 | 20 | 113 | 4 | 97 | 5.45e-19 | 76.6 |
| MS.gene57576.t1 | AT3G05725 | 53.571 | 56 | 25 | 1 | 66 | 121 | 69 | 123 | 2.90e-16 | 69.7 |
| MS.gene57576.t1 | AT3G62640 | 48.077 | 52 | 27 | 0 | 70 | 121 | 59 | 110 | 8.48e-15 | 65.5 |
| MS.gene57576.t1 | AT2G47480 | 45.902 | 61 | 33 | 0 | 60 | 120 | 48 | 108 | 9.54e-15 | 65.5 |
| MS.gene57576.t1 | AT1G72720 | 43.956 | 91 | 42 | 3 | 33 | 121 | 42 | 125 | 1.90e-14 | 65.1 |
| MS.gene57576.t1 | AT4G09890 | 59.459 | 37 | 15 | 0 | 78 | 114 | 39 | 75 | 8.67e-11 | 54.7 |
Find 22 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTGTTACCAATCTGGTTT+TGG | 0.243522 | 8.4:+9137826 | None:intergenic |
| CCTTCCATCAGCGTAGGATT+TGG | 0.309332 | 8.4:+9137950 | None:intergenic |
| GTAAACTCAAAGGCTCTCTT+AGG | 0.367994 | 8.4:-9137674 | MS.gene57576:CDS |
| TGTTGTTGTTGTTACCAATC+TGG | 0.372889 | 8.4:+9137820 | None:intergenic |
| AAGAAAGGTAAATCCACAAA+TGG | 0.395506 | 8.4:-9137783 | MS.gene57576:CDS |
| TACAAGGTTTATTCTGTTGA+AGG | 0.413127 | 8.4:-9137696 | MS.gene57576:CDS |
| TTTGAAGAAGGTGCAGAGTA+AGG | 0.455372 | 8.4:+9137912 | None:intergenic |
| TACTCTGCACCTTCTTCAAA+CGG | 0.463760 | 8.4:-9137909 | MS.gene57576:CDS |
| AGATGCAACAGAGTTGTTCA+TGG | 0.485910 | 8.4:-9137627 | MS.gene57576:CDS |
| AACGATGTTAAGTTCAAGAA+AGG | 0.501790 | 8.4:-9137798 | MS.gene57576:CDS |
| CATCCAACCCAAAACCAGAT+TGG | 0.504703 | 8.4:-9137834 | MS.gene57576:CDS |
| ATGCCTCCTCCGTTTGAAGA+AGG | 0.518627 | 8.4:+9137900 | None:intergenic |
| AGAAAGGTAAATCCACAAAT+GGG | 0.520575 | 8.4:-9137782 | MS.gene57576:CDS |
| CTGCATCCTTCCATCAGCGT+AGG | 0.527015 | 8.4:+9137944 | None:intergenic |
| GCAACAGAGTTGTTCATGGC+TGG | 0.536888 | 8.4:-9137623 | MS.gene57576:CDS |
| TTTGGATCTGAAATCTTCCA+TGG | 0.557041 | 8.4:+9137968 | None:intergenic |
| AGATCCAAATCCTACGCTGA+TGG | 0.558533 | 8.4:-9137954 | MS.gene57576:CDS |
| TCTGTTGAAGGTAAACTCAA+AGG | 0.562516 | 8.4:-9137684 | MS.gene57576:CDS |
| GCACCTTCTTCAAACGGAGG+AGG | 0.581419 | 8.4:-9137903 | MS.gene57576:CDS |
| CCAAATCCTACGCTGATGGA+AGG | 0.584749 | 8.4:-9137950 | MS.gene57576:CDS |
| TCAGTGATCCTGAATTGCAG+AGG | 0.668157 | 8.4:-9137737 | MS.gene57576:CDS |
| TCTGCACCTTCTTCAAACGG+AGG | 0.687076 | 8.4:-9137906 | MS.gene57576:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAAGAGTTGCTAGTTACA+AGG | - | chr8.4:9137874-9137893 | MS.gene57576:CDS | 30.0% | |
| AACGATGTTAAGTTCAAGAA+AGG | - | chr8.4:9137788-9137807 | MS.gene57576:CDS | 30.0% | |
| AAGAAAGGTAAATCCACAAA+TGG | - | chr8.4:9137803-9137822 | MS.gene57576:CDS | 30.0% | |
| AGAAAGGTAAATCCACAAAT+GGG | - | chr8.4:9137804-9137823 | MS.gene57576:CDS | 30.0% | |
| TACAAGGTTTATTCTGTTGA+AGG | - | chr8.4:9137890-9137909 | MS.gene57576:CDS | 30.0% | |
| ATGGGTCAACTTCAAAAAGT+TGG | - | chr8.4:9137822-9137841 | MS.gene57576:CDS | 35.0% | |
| TCTGTTGAAGGTAAACTCAA+AGG | - | chr8.4:9137902-9137921 | MS.gene57576:CDS | 35.0% | |
| ! | TGTTGTTGTTGTTACCAATC+TGG | + | chr8.4:9137769-9137788 | None:intergenic | 35.0% |
| !! | TGTTGTTACCAATCTGGTTT+TGG | + | chr8.4:9137763-9137782 | None:intergenic | 35.0% |
| !!! | CTCTTAGGAAGAGTTTTAAG+TGG | - | chr8.4:9137927-9137946 | MS.gene57576:CDS | 35.0% |
| !!! | CTTTTTTTCCTCTGCAATTC+AGG | + | chr8.4:9137860-9137879 | None:intergenic | 35.0% |
| !!! | GTTGTTACCAATCTGGTTTT+GGG | + | chr8.4:9137762-9137781 | None:intergenic | 35.0% |
| !!! | TTTTGAAGTTGACCCATTTG+TGG | + | chr8.4:9137819-9137838 | None:intergenic | 35.0% |
| AGATGCAACAGAGTTGTTCA+TGG | - | chr8.4:9137959-9137978 | MS.gene57576:CDS | 40.0% | |
| GTAAACTCAAAGGCTCTCTT+AGG | - | chr8.4:9137912-9137931 | MS.gene57576:CDS | 40.0% | |
| TACTCTGCACCTTCTTCAAA+CGG | - | chr8.4:9137677-9137696 | MS.gene57576:CDS | 40.0% | |
| ! | TTTGAAGAAGGTGCAGAGTA+AGG | + | chr8.4:9137677-9137696 | None:intergenic | 40.0% |
| !!! | TTACCAATCTGGTTTTGGGT+TGG | + | chr8.4:9137758-9137777 | None:intergenic | 40.0% |
| AGATCCAAATCCTACGCTGA+TGG | - | chr8.4:9137632-9137651 | MS.gene57576:CDS | 45.0% | |
| CATCCAACCCAAAACCAGAT+TGG | - | chr8.4:9137752-9137771 | MS.gene57576:CDS | 45.0% | |
| TCAGTGATCCTGAATTGCAG+AGG | - | chr8.4:9137849-9137868 | MS.gene57576:CDS | 45.0% | |
| !!! | GTTTTGGGTTGGATGAACAG+AGG | + | chr8.4:9137747-9137766 | None:intergenic | 45.0% |
| ATGCCTCCTCCGTTTGAAGA+AGG | + | chr8.4:9137689-9137708 | None:intergenic | 50.0% | |
| CCAAATCCTACGCTGATGGA+AGG | - | chr8.4:9137636-9137655 | MS.gene57576:CDS | 50.0% | |
| CCTTCCATCAGCGTAGGATT+TGG | + | chr8.4:9137639-9137658 | None:intergenic | 50.0% | |
| GCAACAGAGTTGTTCATGGC+TGG | - | chr8.4:9137963-9137982 | MS.gene57576:CDS | 50.0% | |
| TCTGCACCTTCTTCAAACGG+AGG | - | chr8.4:9137680-9137699 | MS.gene57576:CDS | 50.0% | |
| CTGCATCCTTCCATCAGCGT+AGG | + | chr8.4:9137645-9137664 | None:intergenic | 55.0% | |
| GCACCTTCTTCAAACGGAGG+AGG | - | chr8.4:9137683-9137702 | MS.gene57576:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 9137620 | 9137988 | 9137620 | ID=MS.gene57576 |
| chr8.4 | mRNA | 9137620 | 9137988 | 9137620 | ID=MS.gene57576.t1;Parent=MS.gene57576 |
| chr8.4 | exon | 9137620 | 9137988 | 9137620 | ID=MS.gene57576.t1.exon1;Parent=MS.gene57576.t1 |
| chr8.4 | CDS | 9137620 | 9137988 | 9137620 | ID=cds.MS.gene57576.t1;Parent=MS.gene57576.t1 |
| Gene Sequence |
| Protein sequence |
