AlfalfaGEDB | MS.gene75626

M. sativa cultivar XinJiangDaYe / MS.gene75626

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75626.t1	XP_003601757.1	84.6	195	18	3	1	194	1	184	1.70E-77	298.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75626.t1	G7JBF3	84.6	195	18	3	1	194	1	184	1.2e-77	298.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049230	MS.gene75626	0.828549	8.45E-55	-1.69E-46
MS.gene049378	MS.gene75626	0.833862	4.18E-56	-1.69E-46
MS.gene049419	MS.gene75626	-0.822511	2.28E-53	-1.69E-46
MS.gene049669	MS.gene75626	0.827934	1.19E-54	-1.69E-46
MS.gene050105	MS.gene75626	0.870852	1.07E-66	-1.69E-46
MS.gene050227	MS.gene75626	0.802721	4.91E-49	-1.69E-46
MS.gene050228	MS.gene75626	0.808703	2.72E-50	-1.69E-46
MS.gene050312	MS.gene75626	0.817471	3.24E-52	-1.69E-46
MS.gene050442	MS.gene75626	0.824172	9.32E-54	-1.69E-46
MS.gene050508	MS.gene75626	0.850279	1.88E-60	-1.69E-46
MS.gene050605	MS.gene75626	0.830331	3.12E-55	-1.69E-46
MS.gene050623	MS.gene75626	-0.801574	8.44E-49	-1.69E-46
MS.gene050642	MS.gene75626	0.809257	2.07E-50	-1.69E-46
MS.gene050680	MS.gene75626	0.846945	1.58E-59	-1.69E-46
MS.gene050750	MS.gene75626	0.852323	4.98E-61	-1.69E-46
MS.gene050953	MS.gene75626	0.854632	1.08E-61	-1.69E-46
MS.gene050987	MS.gene75626	0.802968	4.36E-49	-1.69E-46
MS.gene051064	MS.gene75626	0.819159	1.34E-52	-1.69E-46
MS.gene051122	MS.gene75626	0.850181	2.00E-60	-1.69E-46
MS.gene051368	MS.gene75626	0.886224	4.12E-72	-1.69E-46
MS.gene051370	MS.gene75626	0.803795	2.94E-49	-1.69E-46
MS.gene051373	MS.gene75626	0.817532	3.14E-52	-1.69E-46
MS.gene051924	MS.gene75626	-0.809979	1.45E-50	-1.69E-46
MS.gene051926	MS.gene75626	-0.828352	9.43E-55	-1.69E-46
MS.gene051983	MS.gene75626	0.823455	1.37E-53	-1.69E-46
MS.gene05216	MS.gene75626	0.818303	2.10E-52	-1.69E-46
MS.gene052176	MS.gene75626	0.854642	1.07E-61	-1.69E-46
MS.gene052190	MS.gene75626	0.801855	7.39E-49	-1.69E-46
MS.gene052333	MS.gene75626	0.882019	1.48E-70	-1.69E-46
MS.gene052389	MS.gene75626	0.844538	7.11E-59	-1.69E-46
MS.gene052426	MS.gene75626	0.807009	6.23E-50	-1.69E-46
MS.gene052500	MS.gene75626	0.811197	7.90E-51	-1.69E-46
MS.gene052502	MS.gene75626	0.818867	1.57E-52	-1.69E-46
MS.gene052586	MS.gene75626	0.850213	1.96E-60	-1.69E-46
MS.gene052674	MS.gene75626	0.832658	8.34E-56	-1.69E-46
MS.gene052675	MS.gene75626	0.831304	1.80E-55	-1.69E-46
MS.gene052677	MS.gene75626	0.835895	1.29E-56	-1.69E-46
MS.gene052824	MS.gene75626	0.800439	1.44E-48	-1.69E-46
MS.gene052826	MS.gene75626	0.814409	1.56E-51	-1.69E-46
MS.gene052954	MS.gene75626	0.844752	6.22E-59	-1.69E-46
MS.gene052965	MS.gene75626	-0.807578	4.72E-50	-1.69E-46
MS.gene053006	MS.gene75626	0.808455	3.07E-50	-1.69E-46
MS.gene053166	MS.gene75626	0.841889	3.61E-58	-1.69E-46
MS.gene053494	MS.gene75626	0.800135	1.66E-48	-1.69E-46
MS.gene05415	MS.gene75626	0.804488	2.11E-49	-1.69E-46
MS.gene054291	MS.gene75626	-0.833349	5.61E-56	-1.69E-46
MS.gene054405	MS.gene75626	0.846224	2.48E-59	-1.69E-46
MS.gene054468	MS.gene75626	0.810811	9.57E-51	-1.69E-46
MS.gene054479	MS.gene75626	0.835006	2.16E-56	-1.69E-46
MS.gene054559	MS.gene75626	0.809479	1.85E-50	-1.69E-46
MS.gene054657	MS.gene75626	0.812543	4.02E-51	-1.69E-46
MS.gene05510	MS.gene75626	0.835215	1.91E-56	-1.69E-46
MS.gene055225	MS.gene75626	0.854802	9.65E-62	-1.69E-46
MS.gene055367	MS.gene75626	0.839557	1.48E-57	-1.69E-46
MS.gene055701	MS.gene75626	0.900404	7.58E-78	-1.69E-46
MS.gene05595	MS.gene75626	0.817135	3.86E-52	-1.69E-46
MS.gene056087	MS.gene75626	0.821965	3.05E-53	-1.69E-46
MS.gene056480	MS.gene75626	0.808277	3.35E-50	-1.69E-46
MS.gene056506	MS.gene75626	0.887692	1.14E-72	-1.69E-46
MS.gene056779	MS.gene75626	0.816158	6.39E-52	-1.69E-46
MS.gene056873	MS.gene75626	0.813888	2.04E-51	-1.69E-46
MS.gene056979	MS.gene75626	0.810244	1.27E-50	-1.69E-46
MS.gene057026	MS.gene75626	0.814684	1.36E-51	-1.69E-46
MS.gene057156	MS.gene75626	-0.87996	8.13E-70	-1.69E-46
MS.gene057208	MS.gene75626	0.807749	4.34E-50	-1.69E-46
MS.gene05761	MS.gene75626	0.830756	2.46E-55	-1.69E-46
MS.gene058146	MS.gene75626	0.80515	1.53E-49	-1.69E-46
MS.gene058223	MS.gene75626	-0.854922	8.91E-62	-1.69E-46
MS.gene058435	MS.gene75626	0.811095	8.31E-51	-1.69E-46
MS.gene058548	MS.gene75626	0.820342	7.22E-53	-1.69E-46
MS.gene058595	MS.gene75626	0.804858	1.77E-49	-1.69E-46
MS.gene059161	MS.gene75626	0.806317	8.72E-50	-1.69E-46
MS.gene059710	MS.gene75626	0.844511	7.23E-59	-1.69E-46
MS.gene060011	MS.gene75626	0.823144	1.62E-53	-1.69E-46
MS.gene060246	MS.gene75626	0.800401	1.47E-48	-1.69E-46
MS.gene060297	MS.gene75626	0.801466	8.89E-49	-1.69E-46
MS.gene060322	MS.gene75626	0.811802	5.84E-51	-1.69E-46
MS.gene060683	MS.gene75626	0.815334	9.75E-52	-1.69E-46
MS.gene060685	MS.gene75626	0.808763	2.64E-50	-1.69E-46
MS.gene060840	MS.gene75626	0.800585	1.35E-48	-1.69E-46
MS.gene060850	MS.gene75626	0.813786	2.15E-51	-1.69E-46
MS.gene060851	MS.gene75626	0.827414	1.58E-54	-1.69E-46
MS.gene060852	MS.gene75626	0.826551	2.55E-54	-1.69E-46
MS.gene060879	MS.gene75626	0.850486	1.65E-60	-1.69E-46
MS.gene060998	MS.gene75626	0.803174	3.95E-49	-1.69E-46
MS.gene061040	MS.gene75626	-0.820363	7.14E-53	-1.69E-46
MS.gene061114	MS.gene75626	-0.820319	7.30E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75626.t1	MTR_3g085060	84.615	195	18	3	1	194	1	184	9.85e-114	322

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75626.t1	AT1G27300	33.168	202	105	5	1	184	1	190	1.21e-21	88.2

Find 45 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTCATTCCTTTCCGAAATT+TGG	0.127733	3.3:-71085170	None:intergenic
ATTCAGTTTCGTGGTCTTTA+AGG	0.248448	3.3:-71084572	None:intergenic
ATACTCCCAACGGATCTAAA+TGG	0.272675	3.3:-71085026	None:intergenic
ACATCCATGTTGGTCATATC+TGG	0.303699	3.3:-71085146	None:intergenic
GCAGAAGCCACCTGCTTTGA+AGG	0.325878	3.3:-71084801	None:intergenic
AACATGGATGTACCAAATTT+CGG	0.350139	3.3:+71085158	MS.gene75626:CDS
GATAATCAAAGAGCTGAAAA+AGG	0.382164	3.3:+71084887	MS.gene75626:CDS
CGGCGGTTATGGAGCAAGAT+AGG	0.419703	3.3:+71084478	None:intergenic
CCACCTACTCTCTGTCTCTC+AGG	0.446309	3.3:+71084611	MS.gene75626:CDS
TGTTTCTAGCAGCCATGGTC+AGG	0.446396	3.3:+71085070	MS.gene75626:CDS
CTTTGAAGGACTAGATGCTT+CGG	0.454664	3.3:-71084787	None:intergenic
TACTCCCAACGGATCTAAAT+GGG	0.474977	3.3:-71085025	None:intergenic
AACCACCCATTTAGATCCGT+TGG	0.484895	3.3:+71085020	MS.gene75626:CDS
TAGCAGTTGGAAATGTCTAA+AGG	0.502031	3.3:+71084744	MS.gene75626:intron
ACCACCCATTTAGATCCGTT+GGG	0.504389	3.3:+71085021	MS.gene75626:CDS
TCTGCAATTGAGAAAACAAA+AGG	0.510878	3.3:+71084819	MS.gene75626:CDS
CCTGAGAGACAGAGAGTAGG+TGG	0.533620	3.3:-71084611	None:intergenic
AGCATCTAGTCCTTCAAAGC+AGG	0.533942	3.3:+71084791	MS.gene75626:CDS
TGATTGTCCTCACTGTGGCT+TGG	0.535081	3.3:+71084952	MS.gene75626:CDS
CTTAAGAGAAGGAAGAGATG+TGG	0.542676	3.3:-71085120	None:intergenic
AGGATGCATGGGAAGTGAAA+AGG	0.545731	3.3:+71084860	MS.gene75626:CDS
AAGGACTAGATGCTTCGGAT+TGG	0.550420	3.3:-71084782	None:intergenic
GAAATTTGGTACATCCATGT+TGG	0.556305	3.3:-71085156	None:intergenic
TGTCATCAGCATCCTGACCA+TGG	0.563700	3.3:-71085082	None:intergenic
CTCTTTGAACATACTCCCAA+CGG	0.567570	3.3:-71085036	None:intergenic
TGTGGCTTGGCAACTATCAG+AGG	0.572775	3.3:+71084965	MS.gene75626:CDS
AAAGGTGAAACTGTGGATGA+TGG	0.574054	3.3:+71084837	MS.gene75626:CDS
AAAGATGTTTCTAGCAGCCA+TGG	0.580571	3.3:+71085065	MS.gene75626:CDS
TGTGGATGATGGAGGATGCA+TGG	0.587950	3.3:+71084848	MS.gene75626:CDS
ATATCTGGAATCTTAAGAGA+AGG	0.599963	3.3:-71085131	None:intergenic
GTGGATGATGGAGGATGCAT+GGG	0.603480	3.3:+71084849	MS.gene75626:CDS
CGGTTATGGAGCAAGATAGG+AGG	0.604157	3.3:+71084481	None:intergenic
ATCTAGTCCTTCAAAGCAGG+TGG	0.612263	3.3:+71084794	MS.gene75626:CDS
GGATGTACCAAATTTCGGAA+AGG	0.619495	3.3:+71085163	MS.gene75626:CDS
CTTGTGATGAGAGTGAAGGA+TGG	0.629930	3.3:+71084993	MS.gene75626:CDS
AGCAATGATTGTCCTCACTG+TGG	0.630188	3.3:+71084947	MS.gene75626:CDS
GGTGAAACTGTGGATGATGG+AGG	0.632117	3.3:+71084840	MS.gene75626:CDS
CTGAGAGACAGAGAGTAGGT+GGG	0.637959	3.3:-71084610	None:intergenic
GATTCCAGATATGACCAACA+TGG	0.664463	3.3:+71085142	MS.gene75626:CDS
TCCCAACGGATCTAAATGGG+TGG	0.668953	3.3:-71085022	None:intergenic
GAAAACAAAAGGTGAAACTG+TGG	0.678024	3.3:+71084830	MS.gene75626:CDS
GTACCTGAGAGACAGAGAGT+AGG	0.684915	3.3:-71084614	None:intergenic
TTCACTTGTGATGAGAGTGA+AGG	0.685850	3.3:+71084989	MS.gene75626:CDS
CAGTTGGAAATGTCTAAAGG+AGG	0.689175	3.3:+71084747	MS.gene75626:intron
ATAGTTGCCAAGCCACAGTG+AGG	0.743956	3.3:-71084959	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CAATTTGATTTATTAGCAGT+TGG	+	chr3.3:71084731-71084750	MS.gene75626:intron	25.0%
!!!	GAATATGTTTTTGAATTGCT+TGG	-	chr3.3:71084521-71084540	None:intergenic	25.0%
	AACATGGATGTACCAAATTT+CGG	+	chr3.3:71085158-71085177	MS.gene75626:CDS	30.0%
	GATAATCAAAGAGCTGAAAA+AGG	+	chr3.3:71084887-71084906	MS.gene75626:CDS	30.0%
	TCTGCAATTGAGAAAACAAA+AGG	+	chr3.3:71084819-71084838	MS.gene75626:CDS	30.0%
!	ATATCTGGAATCTTAAGAGA+AGG	-	chr3.3:71085134-71085153	None:intergenic	30.0%
	ATTCAGTTTCGTGGTCTTTA+AGG	-	chr3.3:71084575-71084594	None:intergenic	35.0%
	GAAAACAAAAGGTGAAACTG+TGG	+	chr3.3:71084830-71084849	MS.gene75626:CDS	35.0%
	GAAATTTGGTACATCCATGT+TGG	-	chr3.3:71085159-71085178	None:intergenic	35.0%
	TAGCAGTTGGAAATGTCTAA+AGG	+	chr3.3:71084744-71084763	MS.gene75626:intron	35.0%
!!	CTTTTTGGTATTCAGTTTCG+TGG	-	chr3.3:71084584-71084603	None:intergenic	35.0%
	AAAGGTGAAACTGTGGATGA+TGG	+	chr3.3:71084837-71084856	MS.gene75626:CDS	40.0%
	ACATCCATGTTGGTCATATC+TGG	-	chr3.3:71085149-71085168	None:intergenic	40.0%
	ATACTCCCAACGGATCTAAA+TGG	-	chr3.3:71085029-71085048	None:intergenic	40.0%
	CAGTTGGAAATGTCTAAAGG+AGG	+	chr3.3:71084747-71084766	MS.gene75626:intron	40.0%
	CTCTTTGAACATACTCCCAA+CGG	-	chr3.3:71085039-71085058	None:intergenic	40.0%
	CTTAAGAGAAGGAAGAGATG+TGG	-	chr3.3:71085123-71085142	None:intergenic	40.0%
	GATTCCAGATATGACCAACA+TGG	+	chr3.3:71085142-71085161	MS.gene75626:CDS	40.0%
	GGATGTACCAAATTTCGGAA+AGG	+	chr3.3:71085163-71085182	MS.gene75626:CDS	40.0%
	TACTCCCAACGGATCTAAAT+GGG	-	chr3.3:71085028-71085047	None:intergenic	40.0%
	TTCACTTGTGATGAGAGTGA+AGG	+	chr3.3:71084989-71085008	MS.gene75626:CDS	40.0%
!	CTTTGAAGGACTAGATGCTT+CGG	-	chr3.3:71084790-71084809	None:intergenic	40.0%
!!	AAAGATGTTTCTAGCAGCCA+TGG	+	chr3.3:71085065-71085084	MS.gene75626:CDS	40.0%
	AACCACCCATTTAGATCCGT+TGG	+	chr3.3:71085020-71085039	MS.gene75626:CDS	45.0%
	AAGGACTAGATGCTTCGGAT+TGG	-	chr3.3:71084785-71084804	None:intergenic	45.0%
	ACCACCCATTTAGATCCGTT+GGG	+	chr3.3:71085021-71085040	MS.gene75626:CDS	45.0%
	AGCAATGATTGTCCTCACTG+TGG	+	chr3.3:71084947-71084966	MS.gene75626:CDS	45.0%
	AGCATCTAGTCCTTCAAAGC+AGG	+	chr3.3:71084791-71084810	MS.gene75626:CDS	45.0%
	AGGATGCATGGGAAGTGAAA+AGG	+	chr3.3:71084860-71084879	MS.gene75626:CDS	45.0%
	ATCTAGTCCTTCAAAGCAGG+TGG	+	chr3.3:71084794-71084813	MS.gene75626:CDS	45.0%
	CTTGTGATGAGAGTGAAGGA+TGG	+	chr3.3:71084993-71085012	MS.gene75626:CDS	45.0%
	ATAGTTGCCAAGCCACAGTG+AGG	-	chr3.3:71084962-71084981	None:intergenic	50.0%
	CTGAGAGACAGAGAGTAGGT+GGG	-	chr3.3:71084613-71084632	None:intergenic	50.0%
	GTACCTGAGAGACAGAGAGT+AGG	-	chr3.3:71084617-71084636	None:intergenic	50.0%
	TCCCAACGGATCTAAATGGG+TGG	-	chr3.3:71085025-71085044	None:intergenic	50.0%
	TGATTGTCCTCACTGTGGCT+TGG	+	chr3.3:71084952-71084971	MS.gene75626:CDS	50.0%
	TGTCATCAGCATCCTGACCA+TGG	-	chr3.3:71085085-71085104	None:intergenic	50.0%
!	GGTGAAACTGTGGATGATGG+AGG	+	chr3.3:71084840-71084859	MS.gene75626:CDS	50.0%
!	GTAGGTGGGAGAGAGCTTTT+TGG	-	chr3.3:71084599-71084618	None:intergenic	50.0%
!	TGTGGCTTGGCAACTATCAG+AGG	+	chr3.3:71084965-71084984	MS.gene75626:CDS	50.0%
!!	GTGGATGATGGAGGATGCAT+GGG	+	chr3.3:71084849-71084868	MS.gene75626:CDS	50.0%
!!	TGTGGATGATGGAGGATGCA+TGG	+	chr3.3:71084848-71084867	MS.gene75626:CDS	50.0%
!!	TGTTTCTAGCAGCCATGGTC+AGG	+	chr3.3:71085070-71085089	MS.gene75626:CDS	50.0%
	CCACCTACTCTCTGTCTCTC+AGG	+	chr3.3:71084611-71084630	MS.gene75626:CDS	55.0%
	CCTGAGAGACAGAGAGTAGG+TGG	-	chr3.3:71084614-71084633	None:intergenic	55.0%
	GCAGAAGCCACCTGCTTTGA+AGG	-	chr3.3:71084804-71084823	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	71084486	71085190	71084486	ID=MS.gene75626
chr3.3	mRNA	71084486	71085190	71084486	ID=MS.gene75626.t1;Parent=MS.gene75626
chr3.3	exon	71084486	71084632	71084486	ID=MS.gene75626.t1.exon1;Parent=MS.gene75626.t1
chr3.3	CDS	71084486	71084632	71084486	ID=cds.MS.gene75626.t1;Parent=MS.gene75626.t1
chr3.3	exon	71084750	71085190	71084750	ID=MS.gene75626.t1.exon2;Parent=MS.gene75626.t1
chr3.3	CDS	71084750	71085190	71084750	ID=cds.MS.gene75626.t1;Parent=MS.gene75626.t1

Gene Sequence

>MS.gene75626

ATGGAGCAAGATAGGAGGCTTGAACTCATCGACCAAGCAATTCAAAAACATATTCATAACAAAAGTGACAATAATAATAATCATAACCTTAAAGACCACGAAACTGAATACCAAAAAGCTCTCTCCCACCTACTCTCTGTCTCTCAGGTACTTTATGTTCATTTTGCATGTCAATATTATTTATTTACATGTTAATATGTCATATGTTGTCTCTCAAATTGAACACGTTTTATGTTCATCTTGCTCAATTTGATTTATTAGCAGTTGGAAATGTCTAAAGGAGGTGAAACAGTTAACCAATCCGAAGCATCTAGTCCTTCAAAGCAGGTGGCTTCTGCAATTGAGAAAACAAAAGGTGAAACTGTGGATGATGGAGGATGCATGGGAAGTGAAAAGGATGAGATAATCAAAGAGCTGAAAAAGGTGAAGAAGCAGAATTTTGTGACTCATTGTCTTCTTTCAGCAATGATTGTCCTCACTGTGGCTTGGCAACTATCAGAGGTTTCACTTGTGATGAGAGTGAAGGATGGAATAAACCACCCATTTAGATCCGTTGGGAGTATGTTCAAAGAGATAGTGAAAGATGTTTCTAGCAGCCATGGTCAGGATGCTGATGACAAAGAAAACAATGAATCCACATCTCTTCCTTCTCTTAAGATTCCAGATATGACCAACATGGATGTACCAAATTTCGGAAAGGAATGA

Protein sequence

>MS.gene75626.t1

MEQDRRLELIDQAIQKHIHNKSDNNNNHNLKDHETEYQKALSHLLSVSQLEMSKGGETVNQSEASSPSKQVASAIEKTKGETVDDGGCMGSEKDEIIKELKKVKKQNFVTHCLLSAMIVLTVAWQLSEVSLVMRVKDGINHPFRSVGSMFKEIVKDVSSSHGQDADDKENNESTSLPSLKIPDMTNMDVPNFGKE