AlfalfaGEDB | MS.gene88976

M. sativa cultivar XinJiangDaYe / MS.gene88976

KEGG: -
GO: -
PFAM: PF05347.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88976.t1	XP_013466199.1	95.6	137	5	1	1	137	1	136	2.60E-52	214.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88976.t1	I3S747	95.6	137	5	1	1	137	1	136	1.9e-52	214.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04915	MS.gene88976	0.806098	9.70E-50	-1.69E-46
MS.gene049166	MS.gene88976	0.805405	1.36E-49	-1.69E-46
MS.gene04917	MS.gene88976	0.804699	1.91E-49	-1.69E-46
MS.gene049756	MS.gene88976	0.80297	4.36E-49	-1.69E-46
MS.gene04984	MS.gene88976	0.804748	1.86E-49	-1.69E-46
MS.gene049887	MS.gene88976	0.820262	7.53E-53	-1.69E-46
MS.gene050114	MS.gene88976	0.826843	2.17E-54	-1.69E-46
MS.gene050277	MS.gene88976	0.814788	1.29E-51	-1.69E-46
MS.gene050288	MS.gene88976	0.803206	3.89E-49	-1.69E-46
MS.gene050317	MS.gene88976	0.840514	8.31E-58	-1.69E-46
MS.gene050320	MS.gene88976	0.83154	1.58E-55	-1.69E-46
MS.gene050386	MS.gene88976	0.815467	9.11E-52	-1.69E-46
MS.gene050752	MS.gene88976	0.832126	1.13E-55	-1.69E-46
MS.gene050838	MS.gene88976	0.865597	5.26E-65	-1.69E-46
MS.gene051003	MS.gene88976	0.848278	6.79E-60	-1.69E-46
MS.gene051063	MS.gene88976	0.816033	6.81E-52	-1.69E-46
MS.gene051233	MS.gene88976	0.816796	4.60E-52	-1.69E-46
MS.gene051245	MS.gene88976	0.86731	1.50E-65	-1.69E-46
MS.gene051328	MS.gene88976	0.809692	1.67E-50	-1.69E-46
MS.gene051343	MS.gene88976	0.838044	3.63E-57	-1.69E-46
MS.gene051746	MS.gene88976	0.825062	5.75E-54	-1.69E-46
MS.gene051748	MS.gene88976	0.842304	2.81E-58	-1.69E-46
MS.gene051756	MS.gene88976	0.84552	3.86E-59	-1.69E-46
MS.gene051757	MS.gene88976	0.802492	5.47E-49	-1.69E-46
MS.gene051903	MS.gene88976	0.823815	1.13E-53	-1.69E-46
MS.gene052335	MS.gene88976	0.810631	1.05E-50	-1.69E-46
MS.gene052434	MS.gene88976	0.809988	1.44E-50	-1.69E-46
MS.gene052527	MS.gene88976	0.80848	3.03E-50	-1.69E-46
MS.gene052947	MS.gene88976	0.806078	9.80E-50	-1.69E-46
MS.gene053058	MS.gene88976	0.839182	1.85E-57	-1.69E-46
MS.gene053109	MS.gene88976	0.847357	1.22E-59	-1.69E-46
MS.gene053590	MS.gene88976	0.815493	8.99E-52	-1.69E-46
MS.gene05380	MS.gene88976	0.815059	1.12E-51	-1.69E-46
MS.gene053952	MS.gene88976	0.804975	1.67E-49	-1.69E-46
MS.gene054403	MS.gene88976	0.825575	4.35E-54	-1.69E-46
MS.gene054823	MS.gene88976	0.813207	2.88E-51	-1.69E-46
MS.gene055174	MS.gene88976	0.817462	3.26E-52	-1.69E-46
MS.gene055311	MS.gene88976	0.835272	1.85E-56	-1.69E-46
MS.gene055314	MS.gene88976	0.817894	2.60E-52	-1.69E-46
MS.gene055441	MS.gene88976	0.81131	7.46E-51	-1.69E-46
MS.gene056097	MS.gene88976	0.822348	2.49E-53	-1.69E-46
MS.gene056181	MS.gene88976	0.840054	1.10E-57	-1.69E-46
MS.gene056432	MS.gene88976	0.812776	3.58E-51	-1.69E-46
MS.gene056580	MS.gene88976	0.83943	1.59E-57	-1.69E-46
MS.gene056631	MS.gene88976	0.804846	1.78E-49	-1.69E-46
MS.gene056752	MS.gene88976	0.847702	9.78E-60	-1.69E-46
MS.gene056787	MS.gene88976	0.843011	1.82E-58	-1.69E-46
MS.gene056849	MS.gene88976	0.811052	8.49E-51	-1.69E-46
MS.gene056986	MS.gene88976	0.803367	3.61E-49	-1.69E-46
MS.gene057057	MS.gene88976	0.855591	5.69E-62	-1.69E-46
MS.gene057094	MS.gene88976	0.84701	1.52E-59	-1.69E-46
MS.gene057140	MS.gene88976	0.804502	2.09E-49	-1.69E-46
MS.gene057234	MS.gene88976	0.85907	5.33E-63	-1.69E-46
MS.gene057451	MS.gene88976	-0.838738	2.41E-57	-1.69E-46
MS.gene057452	MS.gene88976	-0.875866	2.20E-68	-1.69E-46
MS.gene05781	MS.gene88976	0.822907	1.84E-53	-1.69E-46
MS.gene057999	MS.gene88976	0.821893	3.17E-53	-1.69E-46
MS.gene058054	MS.gene88976	0.820208	7.74E-53	-1.69E-46
MS.gene058055	MS.gene88976	0.839198	1.83E-57	-1.69E-46
MS.gene058074	MS.gene88976	0.801497	8.76E-49	-1.69E-46
MS.gene058182	MS.gene88976	0.827669	1.38E-54	-1.69E-46
MS.gene058238	MS.gene88976	0.851221	1.02E-60	-1.69E-46
MS.gene058638	MS.gene88976	0.803306	3.71E-49	-1.69E-46
MS.gene058639	MS.gene88976	0.818234	2.18E-52	-1.69E-46
MS.gene058646	MS.gene88976	0.832504	9.10E-56	-1.69E-46
MS.gene058654	MS.gene88976	0.847133	1.40E-59	-1.69E-46
MS.gene058758	MS.gene88976	0.820446	6.83E-53	-1.69E-46
MS.gene058906	MS.gene88976	0.836756	7.77E-57	-1.69E-46
MS.gene059171	MS.gene88976	0.848667	5.30E-60	-1.69E-46
MS.gene06001	MS.gene88976	0.82927	5.66E-55	-1.69E-46
MS.gene060084	MS.gene88976	0.806377	8.47E-50	-1.69E-46
MS.gene06010	MS.gene88976	0.801872	7.34E-49	-1.69E-46
MS.gene06011	MS.gene88976	0.80918	2.15E-50	-1.69E-46
MS.gene060296	MS.gene88976	0.801268	9.76E-49	-1.69E-46
MS.gene060297	MS.gene88976	0.808619	2.83E-50	-1.69E-46
MS.gene060463	MS.gene88976	0.802864	4.58E-49	-1.69E-46
MS.gene060465	MS.gene88976	0.814137	1.80E-51	-1.69E-46
MS.gene060563	MS.gene88976	0.829271	5.65E-55	-1.69E-46
MS.gene060702	MS.gene88976	0.811991	5.31E-51	-1.69E-46
MS.gene060869	MS.gene88976	0.830347	3.09E-55	-1.69E-46
MS.gene060870	MS.gene88976	0.804056	2.59E-49	-1.69E-46
MS.gene061006	MS.gene88976	0.810292	1.24E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88976.t1	MTR_1g026078	91.875	160	6	3	1	154	1	159	2.97e-94	270

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88976.t1	AT1G76060	65.101	149	42	4	4	144	8	154	8.03e-58	177

Find 33 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGACACCGTGGAAGAGCTTC+TGG	0.320024	1.4:-264624	MS.gene88976:CDS
ATTGTCACTCGGTTGCTTAT+CGG	0.353596	1.4:-264371	MS.gene88976:CDS
CCGCCACGCGCAACCGTTTG+CGG	0.355220	1.4:+264664	None:intergenic
ACGATGCCAAAGGCGTTCTA+TGG	0.381120	1.4:-264460	MS.gene88976:CDS
CAGTGCTTCTCGGCGCGTGC+TGG	0.390621	1.4:+264529	None:intergenic
GAGGAAAATGGCGAAGCCTT+TGG	0.391236	1.4:-264709	MS.gene88976:CDS
AGAAGCTCTTCCACGGTGTC+AGG	0.435099	1.4:+264626	None:intergenic
TGTAGAGACTCAGTGCTTCT+CGG	0.445236	1.4:+264519	None:intergenic
CGTCGCGGTGGCAGTGGCAG+TGG	0.450220	1.4:-264290	MS.gene88976:CDS
AAGGATCGTCGCGGTGGCAG+TGG	0.467706	1.4:-264296	MS.gene88976:CDS
AAGAAGAAGAGTTGCTGAAT+CGG	0.468368	1.4:-264562	MS.gene88976:CDS
TGCTAGGTTTGAGACGGATC+CGG	0.469269	1.4:-264396	MS.gene88976:CDS
GTAAAGAGTTTGAGCTTGCT+AGG	0.476524	1.4:-264412	MS.gene88976:CDS
ATCACGCCATAGAACGCCTT+TGG	0.477887	1.4:+264454	None:intergenic
AGCAACCGAGTGACAATCTC+CGG	0.496816	1.4:+264377	None:intergenic
CGCGGTGGCAGTGGCAGTGG+CGG	0.502495	1.4:-264287	MS.gene88976:CDS
TTCACATGGCACGATGCCAA+AGG	0.565614	1.4:-264470	MS.gene88976:CDS
GTTTCAGATTCGAATTCCAA+AGG	0.567422	1.4:+264693	None:intergenic
TGAGCTTGCTAGGTTTGAGA+CGG	0.572947	1.4:-264402	MS.gene88976:CDS
CCGTTCCAGAAGCTCTTCCA+CGG	0.587827	1.4:+264619	None:intergenic
CCGCAAACGGTTGCGCGTGG+CGG	0.594281	1.4:-264664	MS.gene88976:CDS
GTCACTCGGTTGCTTATCGG+TGG	0.599413	1.4:-264368	MS.gene88976:CDS
CCGTGGAAGAGCTTCTGGAA+CGG	0.622869	1.4:-264619	MS.gene88976:CDS
AAACCGCAAACGGTTGCGCG+TGG	0.625582	1.4:-264667	MS.gene88976:CDS
GTTGCTTATCGGTGGACGTG+AGG	0.631369	1.4:-264360	MS.gene88976:CDS
CAACGCTATTGATAAGCTTG+CGG	0.647873	1.4:-264330	MS.gene88976:CDS
AAGAAGAGTTGCTGAATCGG+CGG	0.650336	1.4:-264559	MS.gene88976:CDS
ATTCAGAAGGATCGTCGCGG+TGG	0.657849	1.4:-264302	MS.gene88976:CDS
GCTTGCGGAACAGATTCAGA+AGG	0.661253	1.4:-264315	MS.gene88976:CDS
CGGATCCGGAGATTGTCACT+CGG	0.686306	1.4:-264382	MS.gene88976:CDS
ACACGACGGTCCTGACACCG+TGG	0.698737	1.4:-264636	MS.gene88976:CDS
GCGTGGCGGTTATTACACGA+CGG	0.720396	1.4:-264650	MS.gene88976:CDS
CAGATTCAGAAGGATCGTCG+CGG	0.756987	1.4:-264305	MS.gene88976:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGAAGAAGAGTTGCTGAAT+CGG	-	chr1.4:264422-264441	MS.gene88976:CDS	35.0%
	GTTTCAGATTCGAATTCCAA+AGG	+	chr1.4:264294-264313	None:intergenic	35.0%
	ATTGTCACTCGGTTGCTTAT+CGG	-	chr1.4:264613-264632	MS.gene88976:CDS	40.0%
	CAACGCTATTGATAAGCTTG+CGG	-	chr1.4:264654-264673	MS.gene88976:CDS	40.0%
	CTGAAACCAAAAACCGCAAA+CGG	-	chr1.4:264307-264326	MS.gene88976:CDS	40.0%
!	GTAAAGAGTTTGAGCTTGCT+AGG	-	chr1.4:264572-264591	MS.gene88976:CDS	40.0%
!	GTGCTTCACGATTTTTCACA+TGG	-	chr1.4:264500-264519	MS.gene88976:CDS	40.0%
	AAGAAGAGTTGCTGAATCGG+CGG	-	chr1.4:264425-264444	MS.gene88976:CDS	45.0%
	TGTAGAGACTCAGTGCTTCT+CGG	+	chr1.4:264468-264487	None:intergenic	45.0%
!!	TGAGCTTGCTAGGTTTGAGA+CGG	-	chr1.4:264582-264601	MS.gene88976:CDS	45.0%
	AGCAACCGAGTGACAATCTC+CGG	+	chr1.4:264610-264629	None:intergenic	50.0%
	ATCACGCCATAGAACGCCTT+TGG	+	chr1.4:264533-264552	None:intergenic	50.0%
	CAGATTCAGAAGGATCGTCG+CGG	-	chr1.4:264679-264698	MS.gene88976:CDS	50.0%
	GAGGAAAATGGCGAAGCCTT+TGG	-	chr1.4:264275-264294	MS.gene88976:CDS	50.0%
	GCTTGCGGAACAGATTCAGA+AGG	-	chr1.4:264669-264688	MS.gene88976:CDS	50.0%
	TTCACATGGCACGATGCCAA+AGG	-	chr1.4:264514-264533	MS.gene88976:CDS	50.0%
!	TGCTAGGTTTGAGACGGATC+CGG	-	chr1.4:264588-264607	MS.gene88976:CDS	50.0%
!!	ACGATGCCAAAGGCGTTCTA+TGG	-	chr1.4:264524-264543	MS.gene88976:CDS	50.0%
	AGAAGCTCTTCCACGGTGTC+AGG	+	chr1.4:264361-264380	None:intergenic	55.0%
	ATTCAGAAGGATCGTCGCGG+TGG	-	chr1.4:264682-264701	MS.gene88976:CDS	55.0%
	CCGTTCCAGAAGCTCTTCCA+CGG	+	chr1.4:264368-264387	None:intergenic	55.0%
	CGGATCCGGAGATTGTCACT+CGG	-	chr1.4:264602-264621	MS.gene88976:CDS	55.0%
	GCGTGGCGGTTATTACACGA+CGG	-	chr1.4:264334-264353	MS.gene88976:CDS	55.0%
	GTCACTCGGTTGCTTATCGG+TGG	-	chr1.4:264616-264635	MS.gene88976:CDS	55.0%
	GTTGCTTATCGGTGGACGTG+AGG	-	chr1.4:264624-264643	MS.gene88976:CDS	55.0%
	TGACACCGTGGAAGAGCTTC+TGG	-	chr1.4:264360-264379	MS.gene88976:CDS	55.0%
!	CCGTGGAAGAGCTTCTGGAA+CGG	-	chr1.4:264365-264384	MS.gene88976:CDS	55.0%
!	GCGCAACCGTTTGCGGTTTT+TGG	+	chr1.4:264316-264335	None:intergenic	55.0%
	AAACCGCAAACGGTTGCGCG+TGG	-	chr1.4:264317-264336	MS.gene88976:CDS	60.0%
	AAGGATCGTCGCGGTGGCAG+TGG	-	chr1.4:264688-264707	MS.gene88976:CDS	65.0%
	ACACGACGGTCCTGACACCG+TGG	-	chr1.4:264348-264367	MS.gene88976:CDS	65.0%
	CAGTGCTTCTCGGCGCGTGC+TGG	+	chr1.4:264458-264477	None:intergenic	70.0%
	CCGCAAACGGTTGCGCGTGG+CGG	-	chr1.4:264320-264339	MS.gene88976:CDS	70.0%
	CCGCCACGCGCAACCGTTTG+CGG	+	chr1.4:264323-264342	None:intergenic	70.0%
	CGCGGTGGCAGTGGCAGTGG+CGG	-	chr1.4:264697-264716	MS.gene88976:CDS	75.0%
	CGTCGCGGTGGCAGTGGCAG+TGG	-	chr1.4:264694-264713	MS.gene88976:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	264271	264735	264271	ID=MS.gene88976
chr1.4	mRNA	264271	264735	264271	ID=MS.gene88976.t1;Parent=MS.gene88976
chr1.4	exon	264271	264735	264271	ID=MS.gene88976.t1.exon1;Parent=MS.gene88976.t1
chr1.4	CDS	264271	264735	264271	ID=cds.MS.gene88976.t1;Parent=MS.gene88976.t1

Gene Sequence

>MS.gene88976

ATGAGAGGAAAATGGCGAAGCCTTTGGAATTCGAATCTGAAACCAAAAACCGCAAACGGTTGCGCGTGGCGGTTATTACACGACGGTCCTGACACCGTGGAAGAGCTTCTGGAACGGCATCTAGTGAAGAAAGTTATCAACGATGATGAAGAAGAAGAAGAGTTGCTGAATCGGCGGCGACTCACCAGCACGCGCCGAGAAGCACTGAGTCTCTACAGAGACATTCTTCGTGCTTCACGATTTTTCACATGGCACGATGCCAAAGGCGTTCTATGGCGTGATCTTCTTAGAGATAGCGCTCGTAAAGAGTTTGAGCTTGCTAGGTTTGAGACGGATCCGGAGATTGTCACTCGGTTGCTTATCGGTGGACGTGAGGCTGTCAACAACGCTATTGATAAGCTTGCGGAACAGATTCAGAAGGATCGTCGCGGTGGCAGTGGCAGTGGCGGTTTTGATCAAACGTGA

Protein sequence

>MS.gene88976.t1

MRGKWRSLWNSNLKPKTANGCAWRLLHDGPDTVEELLERHLVKKVINDDEEEEELLNRRRLTSTRREALSLYRDILRASRFFTWHDAKGVLWRDLLRDSARKEFELARFETDPEIVTRLLIGGREAVNNAIDKLAEQIQKDRRGGSGSGGFDQT