AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene36818


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36818.t1 MTR_8g013990 95.385 195 8 1 1 195 1 194 6.80e-137 380
MS.gene36818.t1 MTR_2g101140 66.332 199 58 5 3 195 1 196 2.37e-84 248
MS.gene36818.t1 MTR_8g021190 46.212 132 58 5 61 185 29 154 4.28e-31 111
MS.gene36818.t1 MTR_1g103550 47.778 90 39 3 62 148 2 86 3.48e-23 90.1
MS.gene36818.t1 MTR_3g105340 59.091 66 26 1 59 123 8 73 3.74e-22 87.4
MS.gene36818.t1 MTR_5g006430 43.750 96 45 3 62 154 2 91 2.34e-21 84.7
MS.gene36818.t1 MTR_3g109860 34.211 152 87 4 31 179 4 145 6.13e-19 79.7
MS.gene36818.t1 MTR_4g125030 47.222 72 37 1 53 123 13 84 1.43e-17 75.1
MS.gene36818.t1 MTR_4g125010 37.234 94 52 2 62 148 24 117 1.53e-17 76.3
MS.gene36818.t1 MTR_1g014930 40.217 92 47 2 47 134 1 88 9.84e-16 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36818.t1 AT5G25840 50.543 184 54 5 35 195 4 173 2.52e-53 168
MS.gene36818.t1 AT1G79770 52.903 155 59 3 35 189 27 167 1.26e-46 151
MS.gene36818.t1 AT4G14819 42.727 110 51 3 62 171 2 99 1.70e-23 90.1
MS.gene36818.t1 AT2G25780 36.774 155 83 4 35 184 7 151 2.23e-22 88.6
MS.gene36818.t1 AT3G22540 40.449 89 50 1 62 150 2 87 2.19e-21 84.7
MS.gene36818.t1 AT1G72510 39.706 136 67 7 62 185 33 165 1.19e-20 84.7
MS.gene36818.t1 AT1G72510 39.706 136 67 7 62 185 33 165 1.19e-20 84.7
MS.gene36818.t1 AT1G54095 39.706 136 69 5 61 185 14 147 3.25e-19 80.5
MS.gene36818.t1 AT5G20670 33.582 134 82 3 59 185 19 152 4.36e-19 80.1
MS.gene36818.t1 AT2G09970 34.058 138 73 7 62 185 33 166 3.97e-16 72.8

Find 42 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAACATGGATGGGAAAATT+AGG 0.194429 8.3:-74810997 MS.gene36818:CDS
CGACATTTCCTTCGAGTTGA+TGG 0.257929 8.3:-74811329 MS.gene36818:CDS
CCTCCTCCTTTCTTGTCCCT+TGG 0.275546 8.3:+74810959 None:intergenic
AAAATAGATAGATAGTTAAT+TGG 0.284549 8.3:+74810868 None:intergenic
TGTGTTAAGTTTAACAAATA+TGG 0.300385 8.3:-74811076 MS.gene36818:CDS
TATATTTCTTATCATGGTTA+TGG 0.301068 8.3:-74811461 None:intergenic
GCTTGTGCTTGAAACAAAAC+TGG 0.379794 8.3:+74811043 None:intergenic
GACAAGAAAGGAGGAGGAGG+AGG 0.400333 8.3:-74810953 MS.gene36818:CDS
GAAAGTTTCGTTGGTTTGTT+TGG 0.408422 8.3:+74811382 None:intergenic
TAACATGGATGGGAAAATTA+GGG 0.434099 8.3:-74810996 MS.gene36818:CDS
ATTTGTGTGCGGTTTATGTT+CGG 0.438573 8.3:-74811173 MS.gene36818:CDS
TTGATCTCTCTTGTAAGTGC+TGG 0.461637 8.3:+74810905 None:intergenic
ACACTTTGCATCTTCAACTT+CGG 0.466067 8.3:+74811267 None:intergenic
TGTCGGAGACCGTTCTTTGA+AGG 0.474473 8.3:+74811347 None:intergenic
TTAAGTTTAACAAATATGGT+AGG 0.490124 8.3:-74811072 MS.gene36818:CDS
AGGGACAAGAAAGGAGGAGG+AGG 0.494514 8.3:-74810956 MS.gene36818:CDS
TGTTGTCAAGTTCATTCTCA+AGG 0.519029 8.3:-74811420 MS.gene36818:CDS
TTCTTTCGCCATCAACTCGA+AGG 0.530628 8.3:+74811321 None:intergenic
TAAGTTTAACAAATATGGTA+GGG 0.538103 8.3:-74811071 MS.gene36818:CDS
TCGGAAGTTGAAAGTTTCGT+TGG 0.539469 8.3:+74811373 None:intergenic
GGAGGCGGTGAAAGAAGAGT+TGG 0.546775 8.3:-74811152 MS.gene36818:CDS
GACCGTTCTTTGAAGGTGGT+CGG 0.559329 8.3:+74811354 None:intergenic
ATCATGGTTATGGCACCTCA+TGG 0.562684 8.3:-74811451 MS.gene36818:CDS
CCAAGGGACAAGAAAGGAGG+AGG 0.566147 8.3:-74810959 MS.gene36818:CDS
CGGAGACCGTTCTTTGAAGG+TGG 0.567308 8.3:+74811350 None:intergenic
GGGCTAAGTCAATTAGTCCA+AGG 0.570684 8.3:-74810976 MS.gene36818:CDS
ATTAGTCCAAGGGACAAGAA+AGG 0.576487 8.3:-74810965 MS.gene36818:CDS
ACGAACACGATCTATGTACT+CGG 0.578833 8.3:+74811213 None:intergenic
CGTGTTCGTAACAAGTTCGA+AGG 0.581435 8.3:-74811199 MS.gene36818:CDS
ACAAGAAAGGAGGAGGAGGA+GGG 0.591395 8.3:-74810952 MS.gene36818:CDS
GGCTAAGTCAATTAGTCCAA+GGG 0.598516 8.3:-74810975 MS.gene36818:CDS
TGTGTGCGGTTTATGTTCGG+AGG 0.599984 8.3:-74811170 MS.gene36818:CDS
TTCCGACCACCTTCAAAGAA+CGG 0.607623 8.3:-74811356 MS.gene36818:CDS
CATCAACTCGAAGGAAATGT+CGG 0.632792 8.3:+74811330 None:intergenic
TCTTCAACTTCGGATATCGA+TGG 0.633325 8.3:+74811277 None:intergenic
GTGTTCGTAACAAGTTCGAA+GGG 0.639090 8.3:-74811198 MS.gene36818:CDS
TTCGAAGGGAAATTTGTGTG+CGG 0.644656 8.3:-74811184 MS.gene36818:CDS
CGAACACGATCTATGTACTC+GGG 0.649900 8.3:+74811214 None:intergenic
GTGCGGTTTATGTTCGGAGG+CGG 0.662587 8.3:-74811167 MS.gene36818:CDS
GATGCAAAGTGTGAGTGTTG+TGG 0.665594 8.3:-74811256 MS.gene36818:CDS
AGTCCAAGGGACAAGAAAGG+AGG 0.666513 8.3:-74810962 MS.gene36818:CDS
GACAACAAACATTCTCCATG+AGG 0.716500 8.3:+74811436 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAGTTTAACAAATATGGTA+GGG - chr8.3:74811260-74811279 MS.gene36818:CDS 20.0%
!! TGTGTTAAGTTTAACAAATA+TGG - chr8.3:74811255-74811274 MS.gene36818:CDS 20.0%
!! TTAAGTTTAACAAATATGGT+AGG - chr8.3:74811259-74811278 MS.gene36818:CDS 20.0%
! AATGTTGAAAAAGAGTAACA+TGG - chr8.3:74811320-74811339 MS.gene36818:CDS 25.0%
AAAGAAGAGTTGGAGAAAAA+TGG - chr8.3:74811189-74811208 MS.gene36818:CDS 30.0%
TAACATGGATGGGAAAATTA+GGG - chr8.3:74811335-74811354 MS.gene36818:CDS 30.0%
TGAAAAAGAGTAACATGGAT+GGG - chr8.3:74811325-74811344 MS.gene36818:CDS 30.0%
TTGAAAAAGAGTAACATGGA+TGG - chr8.3:74811324-74811343 MS.gene36818:CDS 30.0%
AAGAGTTGGAGAAAAATGGT+GGG - chr8.3:74811193-74811212 MS.gene36818:CDS 35.0%
ACACTTTGCATCTTCAACTT+CGG + chr8.3:74811067-74811086 None:intergenic 35.0%
ATTTGTGTGCGGTTTATGTT+CGG - chr8.3:74811158-74811177 MS.gene36818:CDS 35.0%
GTAACATGGATGGGAAAATT+AGG - chr8.3:74811334-74811353 MS.gene36818:CDS 35.0%
TGTTGTCAAGTTCATTCTCA+AGG - chr8.3:74810911-74810930 MS.gene36818:CDS 35.0%
!! GAAAGTTTCGTTGGTTTGTT+TGG + chr8.3:74810952-74810971 None:intergenic 35.0%
ACGAACACGATCTATGTACT+CGG + chr8.3:74811121-74811140 None:intergenic 40.0%
ATTAGTCCAAGGGACAAGAA+AGG - chr8.3:74811366-74811385 MS.gene36818:CDS 40.0%
CATCAACTCGAAGGAAATGT+CGG + chr8.3:74811004-74811023 None:intergenic 40.0%
GAAGAGTTGGAGAAAAATGG+TGG - chr8.3:74811192-74811211 MS.gene36818:CDS 40.0%
GACAACAAACATTCTCCATG+AGG + chr8.3:74810898-74810917 None:intergenic 40.0%
GCTTGTGCTTGAAACAAAAC+TGG + chr8.3:74811291-74811310 None:intergenic 40.0%
GGCTAAGTCAATTAGTCCAA+GGG - chr8.3:74811356-74811375 MS.gene36818:CDS 40.0%
GTGTTCGTAACAAGTTCGAA+GGG - chr8.3:74811133-74811152 MS.gene36818:CDS 40.0%
TCGGAAGTTGAAAGTTTCGT+TGG + chr8.3:74810961-74810980 None:intergenic 40.0%
TCTTCAACTTCGGATATCGA+TGG + chr8.3:74811057-74811076 None:intergenic 40.0%
TTCGAAGGGAAATTTGTGTG+CGG - chr8.3:74811147-74811166 MS.gene36818:CDS 40.0%
TTGATCTCTCTTGTAAGTGC+TGG + chr8.3:74811429-74811448 None:intergenic 40.0%
CGAACACGATCTATGTACTC+GGG + chr8.3:74811120-74811139 None:intergenic 45.0%
CGTGTTCGTAACAAGTTCGA+AGG - chr8.3:74811132-74811151 MS.gene36818:CDS 45.0%
GGGCTAAGTCAATTAGTCCA+AGG - chr8.3:74811355-74811374 MS.gene36818:CDS 45.0%
TTCCGACCACCTTCAAAGAA+CGG - chr8.3:74810975-74810994 MS.gene36818:CDS 45.0%
TTCTTTCGCCATCAACTCGA+AGG + chr8.3:74811013-74811032 None:intergenic 45.0%
! GATGCAAAGTGTGAGTGTTG+TGG - chr8.3:74811075-74811094 MS.gene36818:CDS 45.0%
!! CGACATTTCCTTCGAGTTGA+TGG - chr8.3:74811002-74811021 MS.gene36818:CDS 45.0%
ACAAGAAAGGAGGAGGAGGA+GGG - chr8.3:74811379-74811398 MS.gene36818:CDS 50.0%
AGTCCAAGGGACAAGAAAGG+AGG - chr8.3:74811369-74811388 MS.gene36818:CDS 50.0%
TGTCGGAGACCGTTCTTTGA+AGG + chr8.3:74810987-74811006 None:intergenic 50.0%
TGTGTGCGGTTTATGTTCGG+AGG - chr8.3:74811161-74811180 MS.gene36818:CDS 50.0%
! GACCGTTCTTTGAAGGTGGT+CGG + chr8.3:74810980-74810999 None:intergenic 50.0%
AGGGACAAGAAAGGAGGAGG+AGG - chr8.3:74811375-74811394 MS.gene36818:CDS 55.0%
CCAAGGGACAAGAAAGGAGG+AGG - chr8.3:74811372-74811391 MS.gene36818:CDS 55.0%
CCTCCTCCTTTCTTGTCCCT+TGG + chr8.3:74811375-74811394 None:intergenic 55.0%
GACAAGAAAGGAGGAGGAGG+AGG - chr8.3:74811378-74811397 MS.gene36818:CDS 55.0%
GGAGGCGGTGAAAGAAGAGT+TGG - chr8.3:74811179-74811198 MS.gene36818:CDS 55.0%
GTGCGGTTTATGTTCGGAGG+CGG - chr8.3:74811164-74811183 MS.gene36818:CDS 55.0%
! CGGAGACCGTTCTTTGAAGG+TGG + chr8.3:74810984-74811003 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 74810883 74811470 74810883 ID=MS.gene36818
chr8.3 mRNA 74810883 74811470 74810883 ID=MS.gene36818.t1;Parent=MS.gene36818
chr8.3 exon 74810883 74811470 74810883 ID=MS.gene36818.t1.exon1;Parent=MS.gene36818.t1
chr8.3 CDS 74810883 74811470 74810883 ID=cds.MS.gene36818.t1;Parent=MS.gene36818.t1
Gene Sequence

>MS.gene36818

ATGGTTATGGCACCTCATGGAGAATGTTTGTTGTCAAGTTCATTCTCAAGGAACAACAACAACACACCAAACAAACCAACGAAACTTTCAACTTCCGACCACCTTCAAAGAACGGTCTCCGACATTTCCTTCGAGTTGATGGCGAAAGAAGCAATCGATAATTTAAAACTACCATCGATATCCGAAGTTGAAGATGCAAAGTGTGAGTGTTGTGGCATGTCTGAAGAGTGCACACCCGAGTACATAGATCGTGTTCGTAACAAGTTCGAAGGGAAATTTGTGTGCGGTTTATGTTCGGAGGCGGTGAAAGAAGAGTTGGAGAAAAATGGTGGGAAAAAATTAGAAGAAGCTTTAAACACACATATGAATGCATGTGTTAAGTTTAACAAATATGGTAGGGCTTTTCCAGTTTTGTTTCAAGCACAAGCTATGAAAGAAATGTTGAAAAAGAGTAACATGGATGGGAAAATTAGGGCTAAGTCAATTAGTCCAAGGGACAAGAAAGGAGGAGGAGGAGGGCTTGCTCGTAGTACAAGTTGTATTCCAGCACTTACAAGAGAGATCAACAATATCAAAATAGCCAATTAA

Protein sequence

>MS.gene36818.t1

MVMAPHGECLLSSSFSRNNNNTPNKPTKLSTSDHLQRTVSDISFELMAKEAIDNLKLPSISEVEDAKCECCGMSEECTPEYIDRVRNKFEGKFVCGLCSEAVKEELEKNGGKKLEEALNTHMNACVKFNKYGRAFPVLFQAQAMKEMLKKSNMDGKIRAKSISPRDKKGGGGGLARSTSCIPALTREINNIKIAN