AlfalfaGEDB | MS.gene27100

M. sativa cultivar XinJiangDaYe / MS.gene27100

KEGG: -
GO: GO:0008150 GO:0009987 GO:0019725 GO:0042592 GO:0045454 GO:0050789 GO:0050794 GO:0065007 GO:0065008
PFAM: PF00085.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27100.t1	PNY14915.1	84.8	309	35	3	4	307	3	304	1.10E-142	516.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27100.t1	Q9SGS4	77.1	231	53	0	77	307	72	302	1.9e-103	377.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27100.t1	A0A2K3PI43	84.8	309	35	3	4	307	3	304	7.8e-143	516.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049137	MS.gene27100	0.804419	2.18E-49	-1.69E-46
MS.gene049150	MS.gene27100	0.808946	2.41E-50	-1.69E-46
MS.gene049153	MS.gene27100	0.803777	2.97E-49	-1.69E-46
MS.gene049170	MS.gene27100	0.823509	1.33E-53	-1.69E-46
MS.gene049346	MS.gene27100	0.829182	5.94E-55	-1.69E-46
MS.gene049351	MS.gene27100	0.826661	2.40E-54	-1.69E-46
MS.gene049356	MS.gene27100	0.870048	1.96E-66	-1.69E-46
MS.gene049369	MS.gene27100	0.832682	8.22E-56	-1.69E-46
MS.gene049609	MS.gene27100	0.815772	7.79E-52	-1.69E-46
MS.gene049661	MS.gene27100	0.817535	3.14E-52	-1.69E-46
MS.gene049662	MS.gene27100	0.818975	1.48E-52	-1.69E-46
MS.gene049758	MS.gene27100	0.817385	3.39E-52	-1.69E-46
MS.gene049910	MS.gene27100	0.813202	2.88E-51	-1.69E-46
MS.gene049993	MS.gene27100	0.817508	3.18E-52	-1.69E-46
MS.gene050122	MS.gene27100	0.818052	2.40E-52	-1.69E-46
MS.gene050301	MS.gene27100	0.844948	5.51E-59	-1.69E-46
MS.gene050397	MS.gene27100	0.864304	1.34E-64	-1.69E-46
MS.gene050400	MS.gene27100	0.809921	1.49E-50	-1.69E-46
MS.gene050406	MS.gene27100	0.82382	1.13E-53	-1.69E-46
MS.gene050426	MS.gene27100	-0.876027	1.94E-68	-1.69E-46
MS.gene050432	MS.gene27100	0.803984	2.68E-49	-1.69E-46
MS.gene050464	MS.gene27100	0.831397	1.71E-55	-1.69E-46
MS.gene050529	MS.gene27100	0.854912	8.97E-62	-1.69E-46
MS.gene050593	MS.gene27100	0.831342	1.76E-55	-1.69E-46
MS.gene050670	MS.gene27100	0.92251	8.96E-89	-1.69E-46
MS.gene050812	MS.gene27100	0.833006	6.83E-56	-1.69E-46
MS.gene050971	MS.gene27100	0.841984	3.41E-58	-1.69E-46
MS.gene05105	MS.gene27100	0.907345	5.60E-81	-1.69E-46
MS.gene051255	MS.gene27100	0.806995	6.27E-50	-1.69E-46
MS.gene051360	MS.gene27100	0.833966	3.94E-56	-1.69E-46
MS.gene051387	MS.gene27100	0.853917	1.74E-61	-1.69E-46
MS.gene051525	MS.gene27100	0.903104	4.90E-79	-1.69E-46
MS.gene051582	MS.gene27100	0.84856	5.67E-60	-1.69E-46
MS.gene051605	MS.gene27100	0.814691	1.35E-51	-1.69E-46
MS.gene051666	MS.gene27100	0.800141	1.66E-48	-1.69E-46
MS.gene051860	MS.gene27100	0.831669	1.46E-55	-1.69E-46
MS.gene051983	MS.gene27100	0.80902	2.33E-50	-1.69E-46
MS.gene052011	MS.gene27100	-0.903947	2.05E-79	-1.69E-46
MS.gene052077	MS.gene27100	0.874845	4.91E-68	-1.69E-46
MS.gene052128	MS.gene27100	-0.824401	8.24E-54	-1.69E-46
MS.gene052134	MS.gene27100	0.84008	1.08E-57	-1.69E-46
MS.gene052135	MS.gene27100	0.848123	7.49E-60	-1.69E-46
MS.gene052136	MS.gene27100	0.836068	1.16E-56	-1.69E-46
MS.gene052188	MS.gene27100	0.889973	1.50E-73	-1.69E-46
MS.gene052253	MS.gene27100	0.897029	2.09E-76	-1.69E-46
MS.gene052255	MS.gene27100	0.814552	1.45E-51	-1.69E-46
MS.gene052310	MS.gene27100	0.878629	2.41E-69	-1.69E-46
MS.gene052322	MS.gene27100	0.821212	4.55E-53	-1.69E-46
MS.gene052340	MS.gene27100	0.883725	3.52E-71	-1.69E-46
MS.gene052346	MS.gene27100	0.844046	9.64E-59	-1.69E-46
MS.gene052414	MS.gene27100	0.866093	3.67E-65	-1.69E-46
MS.gene052421	MS.gene27100	0.853911	1.75E-61	-1.69E-46
MS.gene052469	MS.gene27100	0.821473	3.96E-53	-1.69E-46
MS.gene052481	MS.gene27100	0.840698	7.43E-58	-1.69E-46
MS.gene052484	MS.gene27100	0.802275	6.06E-49	-1.69E-46
MS.gene052485	MS.gene27100	0.873948	9.90E-68	-1.69E-46
MS.gene052501	MS.gene27100	0.830965	2.18E-55	-1.69E-46
MS.gene052505	MS.gene27100	0.841828	3.75E-58	-1.69E-46
MS.gene052762	MS.gene27100	0.815666	8.23E-52	-1.69E-46
MS.gene052982	MS.gene27100	0.846764	1.77E-59	-1.69E-46
MS.gene052999	MS.gene27100	0.807495	4.92E-50	-1.69E-46
MS.gene053103	MS.gene27100	0.802653	5.06E-49	-1.69E-46
MS.gene053169	MS.gene27100	0.860545	1.92E-63	-1.69E-46
MS.gene053209	MS.gene27100	0.852946	3.31E-61	-1.69E-46
MS.gene053330	MS.gene27100	0.811133	8.15E-51	-1.69E-46
MS.gene05335	MS.gene27100	0.817781	2.76E-52	-1.69E-46
MS.gene053449	MS.gene27100	0.823399	1.41E-53	-1.69E-46
MS.gene053557	MS.gene27100	0.831969	1.23E-55	-1.69E-46
MS.gene053758	MS.gene27100	0.842068	3.24E-58	-1.69E-46
MS.gene053836	MS.gene27100	0.841623	4.25E-58	-1.69E-46
MS.gene053873	MS.gene27100	0.825888	3.67E-54	-1.69E-46
MS.gene053928	MS.gene27100	0.868799	4.99E-66	-1.69E-46
MS.gene054010	MS.gene27100	0.826179	3.13E-54	-1.69E-46
MS.gene054011	MS.gene27100	0.823054	1.70E-53	-1.69E-46
MS.gene054012	MS.gene27100	0.823513	1.33E-53	-1.69E-46
MS.gene054013	MS.gene27100	0.818987	1.47E-52	-1.69E-46
MS.gene054102	MS.gene27100	0.808979	2.37E-50	-1.69E-46
MS.gene054107	MS.gene27100	0.80379	2.95E-49	-1.69E-46
MS.gene054110	MS.gene27100	0.809414	1.91E-50	-1.69E-46
MS.gene054123	MS.gene27100	0.827727	1.33E-54	-1.69E-46
MS.gene05413	MS.gene27100	0.803475	3.43E-49	-1.69E-46
MS.gene054183	MS.gene27100	0.872055	4.27E-67	-1.69E-46
MS.gene054349	MS.gene27100	0.879798	9.29E-70	-1.69E-46
MS.gene054556	MS.gene27100	0.888693	4.71E-73	-1.69E-46
MS.gene054719	MS.gene27100	0.861577	9.30E-64	-1.69E-46
MS.gene054918	MS.gene27100	0.812532	4.04E-51	-1.69E-46
MS.gene054920	MS.gene27100	0.82868	7.86E-55	-1.69E-46
MS.gene054930	MS.gene27100	0.805843	1.10E-49	-1.69E-46
MS.gene05505	MS.gene27100	0.83993	1.18E-57	-1.69E-46
MS.gene055116	MS.gene27100	0.918712	1.11E-86	-1.69E-46
MS.gene055277	MS.gene27100	0.84149	4.60E-58	-1.69E-46
MS.gene055289	MS.gene27100	0.810243	1.27E-50	-1.69E-46
MS.gene055369	MS.gene27100	0.889463	2.37E-73	-1.69E-46
MS.gene055422	MS.gene27100	0.812272	4.61E-51	-1.69E-46
MS.gene055428	MS.gene27100	0.804908	1.72E-49	-1.69E-46
MS.gene055539	MS.gene27100	0.862691	4.24E-64	-1.69E-46
MS.gene05553	MS.gene27100	0.884751	1.47E-71	-1.69E-46
MS.gene055564	MS.gene27100	0.811169	8.01E-51	-1.69E-46
MS.gene055604	MS.gene27100	0.838787	2.34E-57	-1.69E-46
MS.gene055666	MS.gene27100	0.817918	2.57E-52	-1.69E-46
MS.gene055741	MS.gene27100	0.873674	1.22E-67	-1.69E-46
MS.gene055744	MS.gene27100	0.88339	4.67E-71	-1.69E-46
MS.gene055859	MS.gene27100	0.803123	4.05E-49	-1.69E-46
MS.gene055909	MS.gene27100	0.845507	3.89E-59	-1.69E-46
MS.gene055911	MS.gene27100	0.851804	6.99E-61	-1.69E-46
MS.gene05594	MS.gene27100	0.838584	2.64E-57	-1.69E-46
MS.gene05602	MS.gene27100	0.809075	2.26E-50	-1.69E-46
MS.gene056191	MS.gene27100	0.850354	1.79E-60	-1.69E-46
MS.gene056337	MS.gene27100	0.816954	4.24E-52	-1.69E-46
MS.gene056407	MS.gene27100	0.892107	2.15E-74	-1.69E-46
MS.gene056410	MS.gene27100	0.810209	1.29E-50	-1.69E-46
MS.gene056511	MS.gene27100	0.878579	2.51E-69	-1.69E-46
MS.gene056514	MS.gene27100	0.855489	6.09E-62	-1.69E-46
MS.gene056612	MS.gene27100	0.810298	1.24E-50	-1.69E-46
MS.gene056659	MS.gene27100	0.893691	4.97E-75	-1.69E-46
MS.gene056723	MS.gene27100	0.877284	7.11E-69	-1.69E-46
MS.gene056796	MS.gene27100	0.833944	3.99E-56	-1.69E-46
MS.gene056876	MS.gene27100	0.806416	8.31E-50	-1.69E-46
MS.gene056877	MS.gene27100	0.811827	5.76E-51	-1.69E-46
MS.gene056899	MS.gene27100	-0.828301	9.70E-55	-1.69E-46
MS.gene056902	MS.gene27100	0.82656	2.54E-54	-1.69E-46
MS.gene057019	MS.gene27100	0.810723	1.00E-50	-1.69E-46
MS.gene05702	MS.gene27100	0.85442	1.25E-61	-1.69E-46
MS.gene057076	MS.gene27100	0.815391	9.47E-52	-1.69E-46
MS.gene057121	MS.gene27100	0.8078	4.23E-50	-1.69E-46
MS.gene057124	MS.gene27100	0.877387	6.55E-69	-1.69E-46
MS.gene057396	MS.gene27100	0.870167	1.79E-66	-1.69E-46
MS.gene057448	MS.gene27100	0.82874	7.60E-55	-1.69E-46
MS.gene057449	MS.gene27100	0.803863	2.85E-49	-1.69E-46
MS.gene057450	MS.gene27100	0.8197	1.01E-52	-1.69E-46
MS.gene057469	MS.gene27100	0.805018	1.63E-49	-1.69E-46
MS.gene057476	MS.gene27100	0.857134	2.01E-62	-1.69E-46
MS.gene057749	MS.gene27100	0.891377	4.20E-74	-1.69E-46
MS.gene057823	MS.gene27100	0.89366	5.11E-75	-1.69E-46
MS.gene057825	MS.gene27100	0.840309	9.40E-58	-1.69E-46
MS.gene057888	MS.gene27100	0.814999	1.16E-51	-1.69E-46
MS.gene057927	MS.gene27100	0.805731	1.16E-49	-1.69E-46
MS.gene058002	MS.gene27100	0.859757	3.32E-63	-1.69E-46
MS.gene058007	MS.gene27100	0.866593	2.55E-65	-1.69E-46
MS.gene058019	MS.gene27100	0.807856	4.12E-50	-1.69E-46
MS.gene058082	MS.gene27100	0.811356	7.30E-51	-1.69E-46
MS.gene058090	MS.gene27100	0.803747	3.01E-49	-1.69E-46
MS.gene05811	MS.gene27100	0.845456	4.02E-59	-1.69E-46
MS.gene058120	MS.gene27100	0.819652	1.04E-52	-1.69E-46
MS.gene058142	MS.gene27100	0.836768	7.71E-57	-1.69E-46
MS.gene058233	MS.gene27100	0.846871	1.65E-59	-1.69E-46
MS.gene058276	MS.gene27100	0.813647	2.30E-51	-1.69E-46
MS.gene058293	MS.gene27100	0.828221	1.01E-54	-1.69E-46
MS.gene058365	MS.gene27100	0.842367	2.70E-58	-1.69E-46
MS.gene058376	MS.gene27100	0.861039	1.36E-63	-1.69E-46
MS.gene058444	MS.gene27100	0.858023	1.09E-62	-1.69E-46
MS.gene058660	MS.gene27100	0.836286	1.02E-56	-1.69E-46
MS.gene058700	MS.gene27100	0.888262	6.90E-73	-1.69E-46
MS.gene058710	MS.gene27100	0.87241	3.25E-67	-1.69E-46
MS.gene058787	MS.gene27100	0.805922	1.06E-49	-1.69E-46
MS.gene05886	MS.gene27100	0.834874	2.33E-56	-1.69E-46
MS.gene058928	MS.gene27100	0.849399	3.32E-60	-1.69E-46
MS.gene058948	MS.gene27100	0.865515	5.59E-65	-1.69E-46
MS.gene058949	MS.gene27100	0.85038	1.76E-60	-1.69E-46
MS.gene058995	MS.gene27100	0.819534	1.10E-52	-1.69E-46
MS.gene059011	MS.gene27100	0.859161	5.01E-63	-1.69E-46
MS.gene05907	MS.gene27100	0.877886	4.38E-69	-1.69E-46
MS.gene059080	MS.gene27100	0.821242	4.48E-53	-1.69E-46
MS.gene059082	MS.gene27100	0.863185	2.98E-64	-1.69E-46
MS.gene059141	MS.gene27100	0.829334	5.45E-55	-1.69E-46
MS.gene059285	MS.gene27100	0.840988	6.24E-58	-1.69E-46
MS.gene059294	MS.gene27100	0.837245	5.82E-57	-1.69E-46
MS.gene059366	MS.gene27100	0.852622	4.09E-61	-1.69E-46
MS.gene059420	MS.gene27100	0.923745	1.77E-89	-1.69E-46
MS.gene059462	MS.gene27100	0.860764	1.65E-63	-1.69E-46
MS.gene059499	MS.gene27100	0.800512	1.39E-48	-1.69E-46
MS.gene059597	MS.gene27100	0.826206	3.08E-54	-1.69E-46
MS.gene05959	MS.gene27100	0.805191	1.50E-49	-1.69E-46
MS.gene059674	MS.gene27100	0.828222	1.01E-54	-1.69E-46
MS.gene059676	MS.gene27100	0.852827	3.57E-61	-1.69E-46
MS.gene059688	MS.gene27100	0.895354	1.04E-75	-1.69E-46
MS.gene059692	MS.gene27100	0.887814	1.02E-72	-1.69E-46
MS.gene059695	MS.gene27100	0.89162	3.36E-74	-1.69E-46
MS.gene059807	MS.gene27100	0.866252	3.27E-65	-1.69E-46
MS.gene05984	MS.gene27100	0.856439	3.22E-62	-1.69E-46
MS.gene059876	MS.gene27100	0.843553	1.31E-58	-1.69E-46
MS.gene059894	MS.gene27100	0.805487	1.30E-49	-1.69E-46
MS.gene059933	MS.gene27100	0.814313	1.64E-51	-1.69E-46
MS.gene060247	MS.gene27100	0.814337	1.62E-51	-1.69E-46
MS.gene060372	MS.gene27100	0.832059	1.17E-55	-1.69E-46
MS.gene06037	MS.gene27100	0.81678	4.64E-52	-1.69E-46
MS.gene060403	MS.gene27100	0.880969	3.54E-70	-1.69E-46
MS.gene060407	MS.gene27100	0.81688	4.40E-52	-1.69E-46
MS.gene060569	MS.gene27100	0.811457	6.94E-51	-1.69E-46
MS.gene060804	MS.gene27100	0.833097	6.48E-56	-1.69E-46
MS.gene060835	MS.gene27100	0.841928	3.53E-58	-1.69E-46
MS.gene060837	MS.gene27100	0.855471	6.17E-62	-1.69E-46
MS.gene06090	MS.gene27100	0.800568	1.36E-48	-1.69E-46
MS.gene060986	MS.gene27100	0.837017	6.66E-57	-1.69E-46
MS.gene061041	MS.gene27100	0.819467	1.14E-52	-1.69E-46
MS.gene061049	MS.gene27100	0.872204	3.81E-67	-1.69E-46
MS.gene061051	MS.gene27100	-0.835384	1.73E-56	-1.69E-46
MS.gene061154	MS.gene27100	0.925985	8.72E-91	-1.69E-46
MS.gene061159	MS.gene27100	0.833133	6.35E-56	-1.69E-46
MS.gene061214	MS.gene27100	0.809927	1.49E-50	-1.69E-46
MS.gene061215	MS.gene27100	0.808398	3.16E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27100.t1	MTR_1g026125	89.474	304	16	4	10	307	5	298	0.0	533

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27100.t1	AT1G76080	63.924	316	91	6	1	307	1	302	7.58e-137	389

Find 48 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATTGAGATATACCCTTTCT+TGG	0.236936	1.1:+253812	MS.gene27100:CDS
TGTGGTAGGTATGTTGGTTC+AGG	0.274596	1.1:+254317	MS.gene27100:CDS
TGTTGAGTTTCTTCTTGTTA+TGG	0.292877	1.1:+253860	MS.gene27100:CDS
GAGGGATATGGAAGTTGTTC+AGG	0.305188	1.1:+254259	MS.gene27100:CDS
ACATCACCAACAAGCATATC+TGG	0.321339	1.1:-253996	None:intergenic
GTTGAGTTTCTTCTTGTTAT+GGG	0.323733	1.1:+253861	MS.gene27100:CDS
TGAGGGTTTGCATATGAAAT+TGG	0.324749	1.1:-253499	None:intergenic
AGCTGCTGTGCCTGTTGCTT+TGG	0.336628	1.1:-253658	None:intergenic
GAATGAAGGGTAAGGAAGTT+AGG	0.357510	1.1:-253546	None:intergenic
AAGCTTATTGTTCTTGATGT+TGG	0.379209	1.1:+254110	MS.gene27100:CDS
TACTACGGTGATAACCATTC+AGG	0.418710	1.1:+254023	MS.gene27100:CDS
CATCGTTACATGTTCGGCTT+AGG	0.421411	1.1:-253839	None:intergenic
ACATTGTGGGCCATGTGTTA+AGG	0.424051	1.1:+254139	MS.gene27100:CDS
GTGGTAGGTATGTTGGTTCA+GGG	0.432018	1.1:+254318	MS.gene27100:CDS
AACTCATTGGTGAAATTCTC+AGG	0.435220	1.1:+254348	MS.gene27100:CDS
ATTAGAGATGGTAAGATTTG+TGG	0.439626	1.1:+254299	MS.gene27100:CDS
GAAATTGAAGGAGGGATTGA+GGG	0.440827	1.1:-253516	None:intergenic
CGGCTTAGGTCAACCAAGAA+AGG	0.444183	1.1:-253825	None:intergenic
AAGATTTGTGGTAGGTATGT+TGG	0.445880	1.1:+254311	MS.gene27100:CDS
TTAGGATGAGTTGAAATTGA+AGG	0.445917	1.1:-253528	None:intergenic
TGAAATTGAAGGAGGGATTG+AGG	0.455754	1.1:-253517	None:intergenic
GTTGTTATCACCAAAGCAAC+AGG	0.459253	1.1:+253648	MS.gene27100:CDS
ACTCAACATCGTTACATGTT+CGG	0.469979	1.1:-253845	None:intergenic
ATTCATGAAGAAGAAGCTAT+TGG	0.470180	1.1:+253972	MS.gene27100:CDS
AAGAAGAACGAAGAGAATGA+AGG	0.480454	1.1:-253560	None:intergenic
AGGTATGTTGGTTCAGGGAA+AGG	0.482216	1.1:+254323	MS.gene27100:CDS
ATGTTGGTCTTAAACATTGT+GGG	0.490241	1.1:+254126	MS.gene27100:CDS
AGAAGAACGAAGAGAATGAA+GGG	0.507243	1.1:-253559	None:intergenic
AACGAAGAGAATGAAGGGTA+AGG	0.514490	1.1:-253554	None:intergenic
AGAACTCTGCAAAAGAGAAA+AGG	0.515029	1.1:+253905	MS.gene27100:CDS
GGATGAGTTGAAATTGAAGG+AGG	0.516103	1.1:-253525	None:intergenic
AATAAGTAACACGAACTCCT+TGG	0.520146	1.1:-254373	None:intergenic
TCAGGGAAAGGTGAACTCAT+TGG	0.523543	1.1:+254335	MS.gene27100:CDS
CTTGACAGCTTAATCACTGT+CGG	0.540890	1.1:-254167	None:intergenic
GATGAGTTGAAATTGAAGGA+GGG	0.552894	1.1:-253524	None:intergenic
AGATGTTGAGAAACTTATTG+AGG	0.553117	1.1:+254070	MS.gene27100:CDS
ATTCATTCTACTTCTCGGGT+AGG	0.557229	1.1:+253612	MS.gene27100:CDS
ATTGGTCCAGATATGCTTGT+TGG	0.557827	1.1:+253990	MS.gene27100:CDS
GGCTTAGGTCAACCAAGAAA+GGG	0.570139	1.1:-253824	None:intergenic
GATGTTGGTCTTAAACATTG+TGG	0.582468	1.1:+254125	MS.gene27100:CDS
GTTGGTGATGTGTTATACTA+CGG	0.591943	1.1:+254008	MS.gene27100:CDS
GTGAGTGATTGTAGTAGCCA+TGG	0.593491	1.1:-253472	None:intergenic
GAAGTTGTACTACACCTGAA+TGG	0.613386	1.1:-254037	None:intergenic
TGAGTGATTGTAGTAGCCAT+GGG	0.617890	1.1:-253471	None:intergenic
GAGATGGTAAGATTTGTGGT+AGG	0.631571	1.1:+254303	MS.gene27100:CDS
GTCGGATAAACCTTAACACA+TGG	0.636213	1.1:-254149	None:intergenic
GGTGAAATTCTCAGGTACCA+AGG	0.658840	1.1:+254356	MS.gene27100:CDS
CTCTTGTAGAAAGTGAAGTG+TGG	0.692063	1.1:-253939	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAAAATTCATTCTACTTCTC+GGG	+	chr1.1:253608-253627	MS.gene27100:CDS	25.0%
!	CAAAAATTCATTCTACTTCT+CGG	+	chr1.1:253607-253626	MS.gene27100:CDS	25.0%
!	TCTCTAATGAACAAAAAAGT+AGG	-	chr1.1:254287-254306	None:intergenic	25.0%
!!!	ACTTTTTTGTTCATTAGAGA+TGG	+	chr1.1:254287-254306	MS.gene27100:CDS	25.0%
!!!	ATGATACTGTTATTTTTGCA+AGG	+	chr1.1:254198-254217	MS.gene27100:CDS	25.0%
	AAGCTTATTGTTCTTGATGT+TGG	+	chr1.1:254110-254129	MS.gene27100:CDS	30.0%
	AGATGTTGAGAAACTTATTG+AGG	+	chr1.1:254070-254089	MS.gene27100:CDS	30.0%
	ATTAGAGATGGTAAGATTTG+TGG	+	chr1.1:254299-254318	MS.gene27100:CDS	30.0%
	ATTCATGAAGAAGAAGCTAT+TGG	+	chr1.1:253972-253991	MS.gene27100:CDS	30.0%
	TGTTGAGTTTCTTCTTGTTA+TGG	+	chr1.1:253860-253879	MS.gene27100:CDS	30.0%
!	ATAGTTGTATGCAGTTTTTG+AGG	+	chr1.1:254240-254259	MS.gene27100:CDS	30.0%
!	CGAGAAGTAGAATGAATTTT+TGG	-	chr1.1:253609-253628	None:intergenic	30.0%
!	GTTGAGTTTCTTCTTGTTAT+GGG	+	chr1.1:253861-253880	MS.gene27100:CDS	30.0%
!	TAGTTGTATGCAGTTTTTGA+GGG	+	chr1.1:254241-254260	MS.gene27100:CDS	30.0%
!	TTAGGATGAGTTGAAATTGA+AGG	-	chr1.1:253531-253550	None:intergenic	30.0%
!!	ATGTTGGTCTTAAACATTGT+GGG	+	chr1.1:254126-254145	MS.gene27100:CDS	30.0%
!!!	GTTATTTTTGCAAGGATGAA+TGG	+	chr1.1:254206-254225	MS.gene27100:CDS	30.0%
	AACTCATTGGTGAAATTCTC+AGG	+	chr1.1:254348-254367	MS.gene27100:CDS	35.0%
	AAGAAGAACGAAGAGAATGA+AGG	-	chr1.1:253563-253582	None:intergenic	35.0%
	AAGATTTGTGGTAGGTATGT+TGG	+	chr1.1:254311-254330	MS.gene27100:CDS	35.0%
	AATAAGTAACACGAACTCCT+TGG	-	chr1.1:254376-254395	None:intergenic	35.0%
	ACTCAACATCGTTACATGTT+CGG	-	chr1.1:253848-253867	None:intergenic	35.0%
	AGAACTCTGCAAAAGAGAAA+AGG	+	chr1.1:253905-253924	MS.gene27100:CDS	35.0%
	AGAAGAACGAAGAGAATGAA+GGG	-	chr1.1:253562-253581	None:intergenic	35.0%
	CATTGAGATATACCCTTTCT+TGG	+	chr1.1:253812-253831	MS.gene27100:CDS	35.0%
	TGAGGGTTTGCATATGAAAT+TGG	-	chr1.1:253502-253521	None:intergenic	35.0%
!	GATGAGTTGAAATTGAAGGA+GGG	-	chr1.1:253527-253546	None:intergenic	35.0%
!!	GATGTTGGTCTTAAACATTG+TGG	+	chr1.1:254125-254144	MS.gene27100:CDS	35.0%
!!	GTTGGTGATGTGTTATACTA+CGG	+	chr1.1:254008-254027	MS.gene27100:CDS	35.0%
!!	TATGCAGTTTTTGAGGGATA+TGG	+	chr1.1:254247-254266	MS.gene27100:CDS	35.0%
	AACGAAGAGAATGAAGGGTA+AGG	-	chr1.1:253557-253576	None:intergenic	40.0%
	ACATCACCAACAAGCATATC+TGG	-	chr1.1:253999-254018	None:intergenic	40.0%
	ATTCATTCTACTTCTCGGGT+AGG	+	chr1.1:253612-253631	MS.gene27100:CDS	40.0%
	ATTGGTCCAGATATGCTTGT+TGG	+	chr1.1:253990-254009	MS.gene27100:CDS	40.0%
	CTCTTGTAGAAAGTGAAGTG+TGG	-	chr1.1:253942-253961	None:intergenic	40.0%
	CTTGACAGCTTAATCACTGT+CGG	-	chr1.1:254170-254189	None:intergenic	40.0%
	GAAATTGAAGGAGGGATTGA+GGG	-	chr1.1:253519-253538	None:intergenic	40.0%
	GAAGTTGTACTACACCTGAA+TGG	-	chr1.1:254040-254059	None:intergenic	40.0%
	GAATGAAGGGTAAGGAAGTT+AGG	-	chr1.1:253549-253568	None:intergenic	40.0%
	GAGATGGTAAGATTTGTGGT+AGG	+	chr1.1:254303-254322	MS.gene27100:CDS	40.0%
	GTCGGATAAACCTTAACACA+TGG	-	chr1.1:254152-254171	None:intergenic	40.0%
	GTTGTTATCACCAAAGCAAC+AGG	+	chr1.1:253648-253667	MS.gene27100:CDS	40.0%
	TACTACGGTGATAACCATTC+AGG	+	chr1.1:254023-254042	MS.gene27100:CDS	40.0%
	TGAAATTGAAGGAGGGATTG+AGG	-	chr1.1:253520-253539	None:intergenic	40.0%
!	GGATGAGTTGAAATTGAAGG+AGG	-	chr1.1:253528-253547	None:intergenic	40.0%
	ACATTGTGGGCCATGTGTTA+AGG	+	chr1.1:254139-254158	MS.gene27100:CDS	45.0%
	CATCGTTACATGTTCGGCTT+AGG	-	chr1.1:253842-253861	None:intergenic	45.0%
	GAGGGATATGGAAGTTGTTC+AGG	+	chr1.1:254259-254278	MS.gene27100:CDS	45.0%
	GGCTTAGGTCAACCAAGAAA+GGG	-	chr1.1:253827-253846	None:intergenic	45.0%
	GGTGAAATTCTCAGGTACCA+AGG	+	chr1.1:254356-254375	MS.gene27100:CDS	45.0%
	TCAGGGAAAGGTGAACTCAT+TGG	+	chr1.1:254335-254354	MS.gene27100:CDS	45.0%
!!	AGGTATGTTGGTTCAGGGAA+AGG	+	chr1.1:254323-254342	MS.gene27100:CDS	45.0%
!!	GTGGTAGGTATGTTGGTTCA+GGG	+	chr1.1:254318-254337	MS.gene27100:CDS	45.0%
!!	TGTGGTAGGTATGTTGGTTC+AGG	+	chr1.1:254317-254336	MS.gene27100:CDS	45.0%
	CGGCTTAGGTCAACCAAGAA+AGG	-	chr1.1:253828-253847	None:intergenic	50.0%
!	AGCTGCTGTGCCTGTTGCTT+TGG	-	chr1.1:253661-253680	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	253474	254397	253474	ID=MS.gene27100
chr1.1	mRNA	253474	254397	253474	ID=MS.gene27100.t1;Parent=MS.gene27100
chr1.1	exon	253474	254397	253474	ID=MS.gene27100.t1.exon1;Parent=MS.gene27100.t1
chr1.1	CDS	253474	254397	253474	ID=cds.MS.gene27100.t1;Parent=MS.gene27100.t1

Gene Sequence

>MS.gene27100

ATGGCTACTACAATCACTCACTGCACCAATTTCATATGCAAACCCTCAATCCCTCCTTCAATTTCAACTCATCCTAACTTCCTTACCCTTCATTCTCTTCGTTCTTCTTCTTCTTCTTCTTCTTCTTCTTTACCAAAAATTCATTCTACTTCTCGGGTAGGACTGCAGAGACGTGTTGTTATCACCAAAGCAACAGGCACAGCAGCTTCAGATGCTAAGAAGAAAAAAGATGAAAGAGTTCAAAGAGTTCACAGCATAGAAGAGTTCGATGCAGCACTTCAATCTGCAAAAGACAAACTCGTTGTTGTTGAGTACGCTACAAGCGACGACTCAGAAAGCATTGAGATATACCCTTTCTTGGTTGACCTAAGCCGAACATGTAACGATGTTGAGTTTCTTCTTGTTATGGGAGATGAATCAGAGAAAACAAAAGAACTCTGCAAAAGAGAAAAGGTTGATAAAGTACCACACTTCACTTTCTACAAGAGCAAAGAGAAGATTCATGAAGAAGAAGCTATTGGTCCAGATATGCTTGTTGGTGATGTGTTATACTACGGTGATAACCATTCAGGTGTAGTACAACTTCACAACAGAGAAGATGTTGAGAAACTTATTGAGGATCATAAGATTGATCATAAGCTTATTGTTCTTGATGTTGGTCTTAAACATTGTGGGCCATGTGTTAAGGTTTATCCGACAGTGATTAAGCTGTCAAGACAAATGAATGATACTGTTATTTTTGCAAGGATGAATGGTGATGAGAATGATAGTTGTATGCAGTTTTTGAGGGATATGGAAGTTGTTCAGGTTCCTACTTTTTTGTTCATTAGAGATGGTAAGATTTGTGGTAGGTATGTTGGTTCAGGGAAAGGTGAACTCATTGGTGAAATTCTCAGGTACCAAGGAGTTCGTGTTACTTATTAA

Protein sequence

>MS.gene27100.t1

MATTITHCTNFICKPSIPPSISTHPNFLTLHSLRSSSSSSSSSLPKIHSTSRVGLQRRVVITKATGTAASDAKKKKDERVQRVHSIEEFDAALQSAKDKLVVVEYATSDDSESIEIYPFLVDLSRTCNDVEFLLVMGDESEKTKELCKREKVDKVPHFTFYKSKEKIHEEEAIGPDMLVGDVLYYGDNHSGVVQLHNREDVEKLIEDHKIDHKLIVLDVGLKHCGPCVKVYPTVIKLSRQMNDTVIFARMNGDENDSCMQFLRDMEVVQVPTFLFIRDGKICGRYVGSGKGELIGEILRYQGVRVTY