AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene29631


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29631.t1 MTR_5g082490 97.561 82 2 0 10 91 48 129 9.23e-56 168
MS.gene29631.t1 MTR_3g070330 76.543 81 19 0 10 90 71 151 1.09e-41 134
MS.gene29631.t1 MTR_7g068250 72.727 66 18 0 10 75 48 113 2.59e-30 104
MS.gene29631.t1 MTR_3g070330 83.636 55 9 0 10 64 71 125 1.97e-29 102
MS.gene29631.t1 MTR_8g027255 56.250 64 28 0 4 67 38 101 2.65e-23 85.9
MS.gene29631.t1 MTR_2g078670 49.425 87 44 0 5 91 43 129 5.18e-23 85.9
MS.gene29631.t1 MTR_8g058785 65.517 58 20 0 10 67 48 105 8.09e-23 85.5
MS.gene29631.t1 MTR_8g061270 65.517 58 20 0 10 67 48 105 8.09e-23 85.5
MS.gene29631.t1 MTR_4g088505 59.375 64 25 1 4 67 43 105 2.85e-21 81.3
MS.gene29631.t1 MTR_3g091840 53.125 64 30 0 4 67 38 101 1.44e-20 79.0
MS.gene29631.t1 MTR_1g077760 51.852 54 26 0 10 63 1 54 1.35e-17 72.0
MS.gene29631.t1 MTR_1g077750 46.296 54 29 0 4 57 38 91 4.34e-15 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29631.t1 AT2G40110 81.707 82 14 1 11 91 49 130 3.48e-43 137
MS.gene29631.t1 AT3G11230 67.045 88 28 1 4 91 43 129 3.10e-37 122
MS.gene29631.t1 AT3G11230 67.045 88 28 1 4 91 76 162 6.16e-37 122
MS.gene29631.t1 AT3G55890 67.073 82 19 1 10 91 48 121 3.18e-32 108
MS.gene29631.t1 AT3G55890 67.073 82 19 1 10 91 48 121 3.18e-32 108
MS.gene29631.t1 AT2G40110 85.455 55 8 0 11 65 49 103 1.37e-29 102
MS.gene29631.t1 AT3G08990 69.697 66 20 0 10 75 48 113 6.70e-29 100
MS.gene29631.t1 AT3G08990 69.697 66 20 0 10 75 48 113 6.70e-29 100
MS.gene29631.t1 AT3G08990 69.697 66 20 0 10 75 48 113 6.70e-29 100
MS.gene29631.t1 AT5G53940 56.627 83 36 0 9 91 47 129 2.08e-27 97.1
MS.gene29631.t1 AT3G55890 79.630 54 11 0 10 63 48 101 2.55e-26 93.6
MS.gene29631.t1 AT3G55890 79.630 54 11 0 10 63 48 101 2.55e-26 93.6
MS.gene29631.t1 AT3G11230 70.968 62 17 1 4 65 43 103 1.33e-24 89.4
MS.gene29631.t1 AT3G11230 72.131 61 16 1 4 64 76 135 2.23e-24 89.7
MS.gene29631.t1 AT4G27745 52.941 68 32 0 4 71 38 105 7.27e-22 82.4
MS.gene29631.t1 AT4G27745 52.941 68 32 0 4 71 38 105 7.27e-22 82.4
MS.gene29631.t1 AT4G27745 52.941 68 32 0 4 71 38 105 7.27e-22 82.4
MS.gene29631.t1 AT4G27745 52.941 68 32 0 4 71 38 105 7.27e-22 82.4
MS.gene29631.t1 AT2G40110 80.000 35 7 0 11 45 49 83 1.22e-14 63.5
MS.gene29631.t1 AT2G40110 80.000 35 7 0 11 45 49 83 1.22e-14 63.5
MS.gene29631.t1 AT4G27740 40.000 60 36 0 9 68 43 102 4.03e-12 57.4

Find 19 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCCTGATGGCAGCAATTAT+TGG 0.259271 5.1:-64575622 MS.gene29631:CDS
TACAGTGTGAATGTTTCTAT+TGG 0.322176 5.1:-64576148 MS.gene29631:intron
TTGACCAGGTTCAAGGTGTC+TGG 0.365136 5.1:-64575644 MS.gene29631:intron
TCCTGATGGCAGCAATTATT+GGG 0.368015 5.1:-64575621 MS.gene29631:CDS
TGTTTCTATTGGAGAATTAG+AGG 0.372709 5.1:-64576137 MS.gene29631:CDS
ACCCAATAATTGCTGCCATC+AGG 0.450327 5.1:+64575620 None:intergenic
GAGGACAGAAATATGATGAC+TGG 0.475545 5.1:-64576118 MS.gene29631:CDS
TTCAAGGTGTCTGGTCCTGA+TGG 0.507863 5.1:-64575635 MS.gene29631:CDS
TTTCAACACCATGTGTTGCA+TGG 0.544712 5.1:+64575594 None:intergenic
GTCGGTTGTGGATCAATCCT+CGG 0.549786 5.1:-64576067 MS.gene29631:CDS
AAAAGCCAGAAGTACAAAGA+AGG 0.556508 5.1:-64575938 MS.gene29631:CDS
TTTCCACAGCAGACATGGGA+AGG 0.566067 5.1:-64576460 None:intergenic
AAGGAAAATCTGTTCTTGAA+CGG 0.572251 5.1:-64575919 MS.gene29631:intron
GTTGTGGATCAATCCTCGGT+TGG 0.576717 5.1:-64576063 MS.gene29631:CDS
TGGGTTAGCCATGCAACACA+TGG 0.601436 5.1:-64575602 MS.gene29631:CDS
AGGACAGAAATATGATGACT+GGG 0.607783 5.1:-64576117 MS.gene29631:CDS
GAAGGCTTATCTCTTCCGTA+AGG 0.633340 5.1:-64576442 MS.gene29631:intron
GTCTTACATATGTCCAACCG+AGG 0.682634 5.1:+64576050 None:intergenic
GATGACTGGGATGCATACTG+TGG 0.714746 5.1:-64576104 MS.gene29631:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTGATAATAATTAGAACAA+GGG - chr5.1:64576233-64576252 MS.gene29631:intron 15.0%
!!! ACAGTTATGTTTTTTATTCA+TGG - chr5.1:64575757-64575776 MS.gene29631:intron 20.0%
!!! CATTGATAATAATTAGAACA+AGG - chr5.1:64576232-64576251 MS.gene29631:intron 20.0%
! ACTAACTGCAAAATATAATG+CGG + chr5.1:64575814-64575833 None:intergenic 25.0%
!!! ATGTTTTTTATTCATGGCTT+TGG - chr5.1:64575763-64575782 MS.gene29631:intron 25.0%
!!! TGTTTTTTATTCATGGCTTT+GGG - chr5.1:64575764-64575783 MS.gene29631:intron 25.0%
AAGGAAAATCTGTTCTTGAA+CGG - chr5.1:64576101-64576120 MS.gene29631:CDS 30.0%
ACCTTTGATTACAAACTACA+AGG + chr5.1:64575727-64575746 None:intergenic 30.0%
CATTAAGTTAAGTTACCCTT+TGG - chr5.1:64576034-64576053 MS.gene29631:intron 30.0%
GATTTCGGTTTCATTGAAAA+TGG + chr5.1:64575667-64575686 None:intergenic 30.0%
! ATTTTCCTTCTTTGTACTTC+TGG + chr5.1:64576090-64576109 None:intergenic 30.0%
! GTTTGTAATCAAAGGTGTAA+AGG - chr5.1:64575731-64575750 MS.gene29631:intron 30.0%
! TACAGTGTGAATGTTTCTAT+TGG - chr5.1:64575872-64575891 MS.gene29631:intron 30.0%
! TGTTTCTATTGGAGAATTAG+AGG - chr5.1:64575883-64575902 MS.gene29631:intron 30.0%
ACTTAATGCAGCATAGAAAC+AGG + chr5.1:64576022-64576041 None:intergenic 35.0%
AGCGATATATAAGTATGTCG+TGG - chr5.1:64576334-64576353 MS.gene29631:intron 35.0%
AGGACAGAAATATGATGACT+GGG - chr5.1:64575903-64575922 MS.gene29631:intron 35.0%
ATGCAGGACAAAAGGTTAAA+GGG + chr5.1:64575695-64575714 None:intergenic 35.0%
ATGGTGAAACCAAAGTTATC+AGG + chr5.1:64575648-64575667 None:intergenic 35.0%
CAAGGATAACTTACACCTTA+CGG + chr5.1:64575596-64575615 None:intergenic 35.0%
GTGTAAGTTATCCTTGACAT+TGG - chr5.1:64575600-64575619 MS.gene29631:CDS 35.0%
! AAAAGCCAGAAGTACAAAGA+AGG - chr5.1:64576082-64576101 MS.gene29631:CDS 35.0%
! CTTTTGTCCTGCATCATTTT+GGG - chr5.1:64575701-64575720 MS.gene29631:intron 35.0%
! GCCTTGTAGTTTGTAATCAA+AGG - chr5.1:64575723-64575742 MS.gene29631:intron 35.0%
AGTCTCCTGAAAGAAACCAA+AGG + chr5.1:64576053-64576072 None:intergenic 40.0%
CCAAAATGATGCAGGACAAA+AGG + chr5.1:64575703-64575722 None:intergenic 40.0%
GAGGACAGAAATATGATGAC+TGG - chr5.1:64575902-64575921 MS.gene29631:intron 40.0%
GATGCAGGACAAAAGGTTAA+AGG + chr5.1:64575696-64575715 None:intergenic 40.0%
GGTTAAAGGGTGGAAGATTT+CGG + chr5.1:64575682-64575701 None:intergenic 40.0%
GTCTCCTGAAAGAAACCAAA+GGG + chr5.1:64576052-64576071 None:intergenic 40.0%
GTGTTCTGTCCTGATAACTT+TGG - chr5.1:64575636-64575655 MS.gene29631:CDS 40.0%
TAATTACTCTGTCAGTGTCG+TGG - chr5.1:64576269-64576288 MS.gene29631:intron 40.0%
TATAATGCGGCTAGAGCTAA+TGG + chr5.1:64575801-64575820 None:intergenic 40.0%
TATATCGCTCAAAACCCATG+TGG + chr5.1:64576323-64576342 None:intergenic 40.0%
TCCTGATGGCAGCAATTATT+GGG - chr5.1:64576399-64576418 MS.gene29631:intron 40.0%
TGCCAAACATCAACACATGT+CGG - chr5.1:64576295-64576314 MS.gene29631:intron 40.0%
TTTCAACACCATGTGTTGCA+TGG + chr5.1:64576429-64576448 None:intergenic 40.0%
! CCTTTTGTCCTGCATCATTT+TGG - chr5.1:64575700-64575719 MS.gene29631:intron 40.0%
! GAGTTTTTGCTCTTTTGACC+AGG - chr5.1:64576362-64576381 MS.gene29631:intron 40.0%
! GTTACCCTTTGGTTTCTTTC+AGG - chr5.1:64576045-64576064 MS.gene29631:intron 40.0%
!! AGTGAAAGGTACCAATGTCA+AGG + chr5.1:64575614-64575633 None:intergenic 40.0%
ACCCAATAATTGCTGCCATC+AGG + chr5.1:64576403-64576422 None:intergenic 45.0%
AGGACAGAACACGTAGTGAA+AGG + chr5.1:64575628-64575647 None:intergenic 45.0%
CAGGACAAAAGGTTAAAGGG+TGG + chr5.1:64575692-64575711 None:intergenic 45.0%
GAAGGCTTATCTCTTCCGTA+AGG - chr5.1:64575578-64575597 MS.gene29631:CDS 45.0%
GGCTAGAGCTAATGGAGATT+CGG + chr5.1:64575793-64575812 None:intergenic 45.0%
GTCCTGATGGCAGCAATTAT+TGG - chr5.1:64576398-64576417 MS.gene29631:intron 45.0%
GTCTTACATATGTCCAACCG+AGG + chr5.1:64575973-64575992 None:intergenic 45.0%
TACAAGGCCCAAAATGATGC+AGG + chr5.1:64575711-64575730 None:intergenic 45.0%
TAGAGCTAATGGAGATTCGG+AGG + chr5.1:64575790-64575809 None:intergenic 45.0%
! CACCGACATGTGTTGATGTT+TGG + chr5.1:64576300-64576319 None:intergenic 45.0%
! GTGGCTGACATTTTCTGTGT+CGG - chr5.1:64575935-64575954 MS.gene29631:CDS 45.0%
! TGCTCTTTTGACCAGGTTCA+AGG - chr5.1:64576369-64576388 MS.gene29631:intron 45.0%
!!! GACATTTTCTGTGTCGGTTG+TGG - chr5.1:64575941-64575960 MS.gene29631:CDS 45.0%
GATGACTGGGATGCATACTG+TGG - chr5.1:64575916-64575935 MS.gene29631:intron 50.0%
GTCGGTTGTGGATCAATCCT+CGG - chr5.1:64575953-64575972 MS.gene29631:CDS 50.0%
GTTGTGGATCAATCCTCGGT+TGG - chr5.1:64575957-64575976 MS.gene29631:intron 50.0%
! TTCAAGGTGTCTGGTCCTGA+TGG - chr5.1:64576385-64576404 MS.gene29631:intron 50.0%
! TTGACCAGGTTCAAGGTGTC+TGG - chr5.1:64576376-64576395 MS.gene29631:intron 50.0%
!! AACACATGTCGGTGCCACAT+GGG - chr5.1:64576306-64576325 MS.gene29631:intron 50.0%
!! TGGGTTAGCCATGCAACACA+TGG - chr5.1:64576418-64576437 MS.gene29631:intron 50.0%
AGGACCAGACACCTTGAACC+TGG + chr5.1:64576383-64576402 None:intergenic 55.0%
!! CAACACATGTCGGTGCCACA+TGG - chr5.1:64576305-64576324 MS.gene29631:intron 55.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 64575574 64576468 64575574 ID=MS.gene29631
chr5.1 mRNA 64575574 64576468 64575574 ID=MS.gene29631.t1;Parent=MS.gene29631
chr5.1 exon 64576443 64576468 64576443 ID=MS.gene29631.t1.exon1;Parent=MS.gene29631.t1
chr5.1 CDS 64576443 64576468 64576443 ID=cds.MS.gene29631.t1;Parent=MS.gene29631.t1
chr5.1 exon 64576057 64576165 64576057 ID=MS.gene29631.t1.exon2;Parent=MS.gene29631.t1
chr5.1 CDS 64576057 64576165 64576057 ID=cds.MS.gene29631.t1;Parent=MS.gene29631.t1
chr5.1 exon 64575920 64575975 64575920 ID=MS.gene29631.t1.exon3;Parent=MS.gene29631.t1
chr5.1 CDS 64575920 64575975 64575920 ID=cds.MS.gene29631.t1;Parent=MS.gene29631.t1
chr5.1 exon 64575574 64575658 64575574 ID=MS.gene29631.t1.exon4;Parent=MS.gene29631.t1
chr5.1 CDS 64575574 64575658 64575574 ID=cds.MS.gene29631.t1;Parent=MS.gene29631.t1
Gene Sequence

>MS.gene29631

ATGGGAAGGCTTATCTCTTCCGTAAGGTGTAAGTTATCCTTGACATTGGTACCTTTCACTACGTGTTCTGTCCTGATAACTTTGGTTTCACCATTTTCAATGAAACCGAAATCTTCCACCCTTTAACCTTTTGTCCTGCATCATTTTGGGCCTTGTAGTTTGTAATCAAAGGTGTAAAGGAGAACAGTTATGTTTTTTATTCATGGCTTTGGGCCTCCGAATCTCCATTAGCTCTAGCCGCATTATATTTTGCAGTTAGTTCTGTTCATGCATTGTAGTTAAAAATTTTCTATGCTTTTACAGTGTGAATGTTTCTATTGGAGAATTAGAGGACAGAAATATGATGACTGGGATGCATACTGTGGCTGACATTTTCTGTGTCGGTTGTGGATCAATCCTCGGTTGGACATATGTAAGACTTATCACGACACAGTTTTTTTTCTCTCCTGTTTCTATGCTGCATTAAGTTAAGTTACCCTTTGGTTTCTTTCAGGAGACTGCTCATGAAAAAAGCCAGAAGTACAAAGAAGGAAAATCTGTTCTTGAACGGTTAGTAGAAGTGATGTGTGAGAAAACATTTTTAATCTAATGTGATTGTGATGTTGTTTTGAGCTATGAAGCACATTCACGAACACTAGACACGACACTGACACGTCGTCATTGATAATAATTAGAACAAGGGAAGAAAATGAATGTAATTACTCTGTCAGTGTCGTGGCAGTGCCAAACATCAACACATGTCGGTGCCACATGGGTTTTGAGCGATATATAAGTATGTCGTGGATTATGAGTTTTTGCTCTTTTGACCAGGTTCAAGGTGTCTGGTCCTGATGGCAGCAATTATTGGGTTAGCCATGCAACACATGGTGTTGAAAGTGATGCAGATGATGCTTAA

Protein sequence

>MS.gene29631.t1

MGRLISSVSVNVSIGELEDRNMMTGMHTVADIFCVGCGSILGWTYETAHEKSQKYKEGKSVLERFKVSGPDGSNYWVSHATHGVESDADDA