AlfalfaGEDB | MS.gene30177

M. sativa cultivar XinJiangDaYe / MS.gene30177

KEGG: K03363
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00400.35 PF12894.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30177.t1	XP_003600092.1	94.3	459	20	2	1	453	1	459	3.70E-256	893.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30177.t1	Q9SZA4	76.2	453	99	4	7	453	8	457	5.3e-203	708.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30177.t1	G7IY01	94.3	459	20	2	1	453	1	459	2.6e-256	893.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene30177	MS.gene62819	PPI
MS.gene71204	MS.gene30177	PPI
MS.gene30177	MS.gene65775	PPI
MS.gene30177	MS.gene80163	PPI
MS.gene77885	MS.gene30177	PPI
MS.gene30177	MS.gene033401	PPI
MS.gene26672	MS.gene30177	PPI
MS.gene97379	MS.gene30177	PPI
MS.gene050456	MS.gene30177	PPI
MS.gene42253	MS.gene30177	PPI
MS.gene058128	MS.gene30177	PPI
MS.gene20160	MS.gene30177	PPI
MS.gene30177	MS.gene06177	PPI
MS.gene30177	MS.gene51278	PPI
MS.gene011448	MS.gene30177	PPI
MS.gene30177	MS.gene87218	PPI
MS.gene066501	MS.gene30177	PPI
MS.gene25244	MS.gene30177	PPI
MS.gene068273	MS.gene30177	PPI
MS.gene014931	MS.gene30177	PPI
MS.gene63085	MS.gene30177	PPI
MS.gene55883	MS.gene30177	PPI
MS.gene056455	MS.gene30177	PPI
MS.gene30177	MS.gene014321	PPI
MS.gene30177	MS.gene064862	PPI
MS.gene30177	MS.gene23881	PPI
MS.gene89986	MS.gene30177	PPI
MS.gene26019	MS.gene30177	PPI
MS.gene30174	MS.gene30177	PPI
MS.gene064762	MS.gene30177	PPI
MS.gene062662	MS.gene30177	PPI
MS.gene049022	MS.gene30177	PPI
MS.gene35613	MS.gene30177	PPI
MS.gene30177	MS.gene84466	PPI
MS.gene69965	MS.gene30177	PPI
MS.gene066500	MS.gene30177	PPI
MS.gene30177	MS.gene008833	PPI
MS.gene21888	MS.gene30177	PPI
MS.gene067321	MS.gene30177	PPI
MS.gene82740	MS.gene30177	PPI
MS.gene90626	MS.gene30177	PPI
MS.gene30177	MS.gene48655	PPI
MS.gene049021	MS.gene30177	PPI
MS.gene30172	MS.gene30177	PPI
MS.gene067322	MS.gene30177	PPI
MS.gene045937	MS.gene30177	PPI
MS.gene060496	MS.gene30177	PPI
MS.gene23852	MS.gene30177	PPI
MS.gene69966	MS.gene30177	PPI
MS.gene97943	MS.gene30177	PPI
MS.gene071113	MS.gene30177	PPI
MS.gene027071	MS.gene30177	PPI
MS.gene30177	MS.gene027072	PPI
MS.gene67157	MS.gene30177	PPI
MS.gene30177	MS.gene65463	PPI
MS.gene69396	MS.gene30177	PPI
MS.gene012203	MS.gene30177	PPI
MS.gene022109	MS.gene30177	PPI
MS.gene34651	MS.gene30177	PPI
MS.gene010910	MS.gene30177	PPI
MS.gene052188	MS.gene30177	PPI
MS.gene025229	MS.gene30177	PPI
MS.gene30177	MS.gene067705	PPI
MS.gene045133	MS.gene30177	PPI
MS.gene063222	MS.gene30177	PPI
MS.gene25515	MS.gene30177	PPI
MS.gene060495	MS.gene30177	PPI
MS.gene07706	MS.gene30177	PPI
MS.gene027074	MS.gene30177	PPI
MS.gene069995	MS.gene30177	PPI
MS.gene30177	MS.gene37226	PPI
MS.gene69687	MS.gene30177	PPI
MS.gene30177	MS.gene99973	PPI
MS.gene30177	MS.gene30174	PPI
MS.gene23811	MS.gene30177	PPI
MS.gene51258	MS.gene30177	PPI
MS.gene23810	MS.gene30177	PPI
MS.gene46611	MS.gene30177	PPI
MS.gene30177	MS.gene064435	PPI
MS.gene51335	MS.gene30177	PPI
MS.gene82021	MS.gene30177	PPI
MS.gene024753	MS.gene30177	PPI
MS.gene81658	MS.gene30177	PPI
MS.gene84465	MS.gene30177	PPI
MS.gene78281	MS.gene30177	PPI
MS.gene55882	MS.gene30177	PPI
MS.gene037950	MS.gene30177	PPI
MS.gene70008	MS.gene30177	PPI
MS.gene068249	MS.gene30177	PPI
MS.gene64441	MS.gene30177	PPI
MS.gene24806	MS.gene30177	PPI
MS.gene004844	MS.gene30177	PPI
MS.gene52355	MS.gene30177	PPI
MS.gene30176	MS.gene30177	PPI
MS.gene24951	MS.gene30177	PPI
MS.gene014419	MS.gene30177	PPI
MS.gene30177	MS.gene037184	PPI
MS.gene066464	MS.gene30177	PPI
MS.gene20161	MS.gene30177	PPI
MS.gene51262	MS.gene30177	PPI
MS.gene050270	MS.gene30177	PPI
MS.gene24922	MS.gene30177	PPI
MS.gene027073	MS.gene30177	PPI
MS.gene33406	MS.gene30177	PPI
MS.gene010912	MS.gene30177	PPI
MS.gene050457	MS.gene30177	PPI
MS.gene30177	MS.gene26021	PPI
MS.gene30177	MS.gene29842	PPI
MS.gene75059	MS.gene30177	PPI
MS.gene34712	MS.gene30177	PPI
MS.gene92674	MS.gene30177	PPI
MS.gene30177	MS.gene32723	PPI
MS.gene064035	MS.gene30177	PPI
MS.gene30177	MS.gene053363	PPI
MS.gene027441	MS.gene30177	PPI
MS.gene81663	MS.gene30177	PPI
MS.gene36842	MS.gene30177	PPI
MS.gene30177	MS.gene75493	PPI
MS.gene99554	MS.gene30177	PPI
MS.gene81660	MS.gene30177	PPI
MS.gene066046	MS.gene30177	PPI
MS.gene30177	MS.gene32726	PPI
MS.gene30177	MS.gene71122	PPI
MS.gene99588	MS.gene30177	PPI
MS.gene027075	MS.gene30177	PPI
MS.gene41291	MS.gene30177	PPI
MS.gene27223	MS.gene30177	PPI
MS.gene66342	MS.gene30177	PPI
MS.gene038519	MS.gene30177	PPI
MS.gene050272	MS.gene30177	PPI
MS.gene06176	MS.gene30177	PPI
MS.gene030294	MS.gene30177	PPI
MS.gene91851	MS.gene30177	PPI
MS.gene30177	MS.gene21888	PPI
MS.gene052956	MS.gene30177	PPI
MS.gene91775	MS.gene30177	PPI
MS.gene29768	MS.gene30177	PPI
MS.gene039682	MS.gene30177	PPI
MS.gene98540	MS.gene30177	PPI
MS.gene043742	MS.gene30177	PPI
MS.gene022502	MS.gene30177	PPI
MS.gene85534	MS.gene30177	PPI
MS.gene042783	MS.gene30177	PPI
MS.gene066467	MS.gene30177	PPI
MS.gene82020	MS.gene30177	PPI
MS.gene21734	MS.gene30177	PPI
MS.gene66343	MS.gene30177	PPI
MS.gene33377	MS.gene30177	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30177.t1	MTR_3g051690	94.336	459	20	2	1	453	1	459	0.0	899
MS.gene30177.t1	MTR_5g008010	89.520	458	39	4	1	453	1	454	0.0	847
MS.gene30177.t1	MTR_7g106870	72.506	451	111	7	12	453	11	457	0.0	679
MS.gene30177.t1	MTR_7g451830	65.324	447	129	9	15	453	3	431	0.0	569
MS.gene30177.t1	MTR_7g047640	65.324	447	129	9	15	453	3	431	0.0	569
MS.gene30177.t1	MTR_7g057540	62.864	447	137	8	14	453	2	426	0.0	539
MS.gene30177.t1	MTR_7g055590	62.780	446	137	8	14	452	2	425	0.0	536
MS.gene30177.t1	MTR_7g053100	60.398	452	149	9	15	453	2	436	0.0	520
MS.gene30177.t1	MTR_7g034625	70.533	319	87	2	128	439	13	331	1.38e-166	472
MS.gene30177.t1	MTR_2g100540	49.886	439	165	12	25	453	4	397	2.96e-128	377
MS.gene30177.t1	MTR_2g100460	56.560	343	135	5	111	451	52	382	2.53e-126	372
MS.gene30177.t1	MTR_2g060710	44.282	411	207	8	33	429	26	428	1.36e-119	357
MS.gene30177.t1	MTR_3g067940	44.048	420	213	7	33	440	57	466	3.32e-115	347
MS.gene30177.t1	MTR_4g102510	42.482	419	209	7	33	432	51	456	6.19e-109	330
MS.gene30177.t1	MTR_4g415070	54.412	68	31	0	378	445	57	124	5.34e-17	82.0
MS.gene30177.t1	MTR_2g012630	27.397	219	148	8	158	373	35	245	1.69e-15	79.7
MS.gene30177.t1	MTR_2g012630	27.397	219	148	8	158	373	35	245	1.99e-15	79.3
MS.gene30177.t1	MTR_1g054300	26.263	297	161	10	178	427	114	399	2.79e-15	78.2
MS.gene30177.t1	MTR_1g054300	26.493	268	169	11	165	425	235	481	9.08e-15	76.6
MS.gene30177.t1	MTR_6g004040	28.326	233	153	10	144	370	19	243	5.11e-15	77.8
MS.gene30177.t1	MTR_5g016370	23.693	287	163	8	147	425	312	550	1.09e-14	76.6
MS.gene30177.t1	MTR_5g016350	23.077	286	178	7	141	425	294	538	8.42e-13	70.9
MS.gene30177.t1	MTR_5g016350	22.997	287	179	7	141	426	230	475	9.23e-13	70.5
MS.gene30177.t1	MTR_4g095058	24.382	283	168	11	147	426	295	534	5.98e-12	68.2
MS.gene30177.t1	MTR_4g095058	24.382	283	168	11	147	426	293	532	7.91e-12	67.8
MS.gene30177.t1	MTR_3g106080	24.026	308	192	10	159	434	326	623	2.38e-11	66.2
MS.gene30177.t1	MTR_1g049000	40.426	94	46	3	204	288	8	100	3.11e-11	62.8
MS.gene30177.t1	MTR_7g086360	23.415	205	150	4	224	426	53	252	3.93e-11	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30177.t1	AT4G33270	76.159	453	99	5	7	453	8	457	0.0	712
MS.gene30177.t1	AT4G33260	76.351	444	96	5	16	453	7	447	0.0	685
MS.gene30177.t1	AT4G33260	74.473	427	100	5	16	436	7	430	0.0	652
MS.gene30177.t1	AT5G27570	67.473	455	132	7	7	453	4	450	0.0	613
MS.gene30177.t1	AT5G27080	66.438	438	132	6	23	453	13	442	0.0	590
MS.gene30177.t1	AT5G26900	64.302	451	146	6	9	453	3	444	0.0	581
MS.gene30177.t1	AT5G27945	61.456	371	123	5	99	453	62	428	1.37e-156	450
MS.gene30177.t1	AT4G22910	42.995	414	211	7	33	432	62	464	4.02e-112	339
MS.gene30177.t1	AT4G11920	42.118	425	212	8	33	438	53	462	9.80e-110	332
MS.gene30177.t1	AT5G13840	51.148	305	146	3	131	435	164	465	1.20e-109	332
MS.gene30177.t1	AT5G13840	51.148	305	146	3	131	435	164	465	1.20e-109	332
MS.gene30177.t1	AT1G11160	27.670	206	132	7	150	344	16	215	4.18e-16	81.6
MS.gene30177.t1	AT1G11160	27.670	206	132	7	150	344	16	215	4.77e-16	81.6
MS.gene30177.t1	AT1G11160	27.670	206	132	7	150	344	16	215	5.08e-16	81.3
MS.gene30177.t1	AT5G08390	29.060	234	136	10	140	370	37	243	7.50e-16	80.9
MS.gene30177.t1	AT1G11160	29.630	162	106	6	185	344	18	173	5.58e-15	78.2
MS.gene30177.t1	AT1G61210	25.676	222	146	8	142	350	6	221	1.12e-14	77.0
MS.gene30177.t1	AT1G61210	25.893	224	143	8	142	350	6	221	1.37e-14	77.0
MS.gene30177.t1	AT1G61210	25.893	224	143	8	142	350	6	221	1.42e-14	77.0
MS.gene30177.t1	AT5G52820	28.629	248	153	12	186	427	244	473	2.87e-14	75.1
MS.gene30177.t1	AT5G23430	28.774	212	138	9	144	350	19	222	1.06e-13	73.9
MS.gene30177.t1	AT5G23430	28.774	212	138	9	144	350	19	222	1.06e-13	73.9
MS.gene30177.t1	AT5G23430	28.774	212	138	9	144	350	19	222	1.11e-13	73.9
MS.gene30177.t1	AT5G25150	24.850	334	212	11	135	440	305	627	1.22e-12	70.5
MS.gene30177.t1	AT5G25150	25.449	334	210	12	135	440	305	627	1.35e-12	70.5
MS.gene30177.t1	AT2G32700	25.203	246	164	11	186	425	553	784	1.36e-12	70.5
MS.gene30177.t1	AT2G32700	25.203	246	164	11	186	425	555	786	1.38e-12	70.5
MS.gene30177.t1	AT2G32700	25.203	246	164	11	186	425	555	786	1.38e-12	70.5
MS.gene30177.t1	AT2G32700	25.203	246	164	11	186	425	555	786	1.38e-12	70.5
MS.gene30177.t1	AT2G32700	25.203	246	164	11	186	425	555	786	1.38e-12	70.5
MS.gene30177.t1	AT2G32700	25.203	246	164	11	186	425	555	786	1.38e-12	70.5
MS.gene30177.t1	AT2G32700	25.203	246	164	11	186	425	574	805	1.43e-12	70.5
MS.gene30177.t1	AT5G67320	25.000	284	165	12	147	426	330	569	2.76e-12	69.3
MS.gene30177.t1	AT3G49660	23.600	250	177	8	182	425	71	312	8.17e-12	66.6
MS.gene30177.t1	AT5G13480	31.319	182	115	7	162	340	218	392	1.26e-11	67.4
MS.gene30177.t1	AT5G13480	26.549	226	155	8	186	408	114	331	3.86e-11	65.9
MS.gene30177.t1	AT5G13480	31.319	182	115	7	162	340	241	415	1.32e-11	67.0
MS.gene30177.t1	AT5G13480	26.549	226	155	8	186	408	137	354	3.79e-11	65.9
MS.gene30177.t1	AT5G13480	31.319	182	115	7	162	340	218	392	1.54e-11	67.0
MS.gene30177.t1	AT5G13480	26.549	226	155	8	186	408	114	331	4.67e-11	65.5
MS.gene30177.t1	AT2G41500	24.797	246	135	8	186	429	342	539	1.75e-11	66.6
MS.gene30177.t1	AT2G41500	24.797	246	135	8	186	429	342	539	1.75e-11	66.6
MS.gene30177.t1	AT5G13480	31.319	182	115	7	162	340	230	404	1.88e-11	66.6
MS.gene30177.t1	AT5G13480	26.549	226	155	8	186	408	126	343	5.53e-11	65.1
MS.gene30177.t1	AT2G33340	25.521	192	135	5	238	426	236	422	3.02e-11	65.9
MS.gene30177.t1	AT2G33340	25.521	192	135	5	238	426	236	422	3.02e-11	65.9
MS.gene30177.t1	AT2G33340	25.521	192	135	5	238	426	236	422	3.34e-11	65.5
MS.gene30177.t1	AT4G02730	26.852	216	145	9	216	426	34	241	6.46e-11	63.9
MS.gene30177.t1	AT1G04510	24.651	215	153	5	238	448	236	445	8.14e-11	64.3
MS.gene30177.t1	AT1G04510	24.651	215	153	5	238	448	236	445	8.89e-11	64.3

Find 129 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGATGAAACCTTGAGGTTT+TGG	0.193519	3.3:+43158105	MS.gene30177:CDS
CTGGAAGGAGAGCACACTTC+TGG	0.221276	3.3:-43156568	None:intergenic
TGACTATTACCTCAATTTAC+TGG	0.257532	3.3:+43157164	MS.gene30177:CDS
GTTGCCACTGTCCAACCATC+TGG	0.273358	3.3:-43158074	None:intergenic
TTACCTCAATTTACTGGATT+GGG	0.283267	3.3:+43157170	MS.gene30177:CDS
AATGTTCTTGCAATTGGATT+AGG	0.295419	3.3:+43157201	MS.gene30177:CDS
CTCCTTCCAGAGAATCTTAC+AGG	0.326925	3.3:+43156581	MS.gene30177:CDS
AGCGCCAATGTTCTTGCAAT+TGG	0.327966	3.3:+43157195	MS.gene30177:CDS
GTTCTTGAAAGCTAGAATTC+TGG	0.331154	3.3:-43156638	None:intergenic
TTCTCTCTAACAATGGCTTC+AGG	0.336781	3.3:+43156209	None:intergenic
GCAGACTTCTGAGAGGATAT+TGG	0.341248	3.3:+43157122	MS.gene30177:intron
GGATTGAAATGGTCGGCTTC+TGG	0.343844	3.3:+43157666	MS.gene30177:CDS
ATGATAATCAACTTTACATC+TGG	0.350065	3.3:+43157712	MS.gene30177:CDS
AACGGCTCAACTCTTGCTTT+TGG	0.355302	3.3:-43158167	None:intergenic
GCGATGAAACCATCAAGTTT+TGG	0.364930	3.3:+43157850	MS.gene30177:CDS
ATTACCTCAATTTACTGGAT+TGG	0.371678	3.3:+43157169	MS.gene30177:CDS
TCTCATTCTTGTTCCACAAC+AGG	0.376647	3.3:-43157929	None:intergenic
AATAATTCTCCGGGGCTTGA+TGG	0.385492	3.3:-43156734	None:intergenic
TAGCTTCCTGTAAGATTCTC+TGG	0.386788	3.3:-43156587	None:intergenic
GCTCCTGATGGCCGTCATAT+TGG	0.389395	3.3:+43157318	MS.gene30177:CDS
ATTTCAATCCGCAAACTTCT+CGG	0.394694	3.3:-43157653	None:intergenic
CCCTATCACATCTGTAAGTT+GGG	0.396604	3.3:+43157296	MS.gene30177:CDS
TCTGCCATCTTCACCATTGA+AGG	0.397901	3.3:-43158008	None:intergenic
CATACCGAATAGATCAGTTA+TGG	0.404214	3.3:+43156486	MS.gene30177:CDS
CTCAGTTGACACTGGGTCTC+AGG	0.408962	3.3:+43157899	MS.gene30177:CDS
CAGAAGCTTTAAACATGAAC+AGG	0.411753	3.3:+43156614	MS.gene30177:CDS
TAGGAAACACATTGTATTTG+TGG	0.412235	3.3:+43157220	MS.gene30177:CDS
CAAGGGAATTTACTAGCTAC+TGG	0.417964	3.3:+43157816	MS.gene30177:CDS
GCCCTATCACATCTGTAAGT+TGG	0.420766	3.3:+43157295	MS.gene30177:CDS
GAGAAGTTTGCGGATTGAAA+TGG	0.425267	3.3:+43157655	MS.gene30177:CDS
CTCAGGTATGTTCCCTGTTG+TGG	0.426699	3.3:+43157916	MS.gene30177:CDS
TAATAGTCATCCACAATGTC+TGG	0.428065	3.3:-43157150	None:intergenic
CTTGCTTGGTGTCCTTTCCA+AGG	0.428743	3.3:+43157798	MS.gene30177:CDS
GTGGGTATTCCAAAACTTGA+TGG	0.428833	3.3:-43157859	None:intergenic
TAGGCAGCGAGTAGGGTCTT+TGG	0.428851	3.3:+43157527	MS.gene30177:CDS
TGGAAGGAGAGCACACTTCT+GGG	0.443148	3.3:-43156567	None:intergenic
GTCGGCTTCTGGTCTACAAT+TGG	0.444050	3.3:+43157677	MS.gene30177:CDS
AAATCCATAACTGATCTATT+CGG	0.445104	3.3:-43156490	None:intergenic
CACAGAATCAGCTAACACTT+TGG	0.446675	3.3:+43157979	MS.gene30177:CDS
GCCCAACTTACAGATGTGAT+AGG	0.450468	3.3:-43157297	None:intergenic
AGGAAACACATTGTATTTGT+GGG	0.452538	3.3:+43157221	MS.gene30177:CDS
CTTCAACAAGGTGGCTTCAC+AGG	0.460645	3.3:+43157745	MS.gene30177:CDS
CTCCGTAAGTGTTCCTGAAG+AGG	0.466289	3.3:-43156272	None:intergenic
TTGCTTGGTGTCCTTTCCAA+GGG	0.471233	3.3:+43157799	MS.gene30177:CDS
ACACCTGAGGAATAATTCTC+CGG	0.481736	3.3:-43156744	None:intergenic
TCATGAGAGAAGAAATCAAC+TGG	0.484334	3.3:-43156676	None:intergenic
ACAACTCTGAAGTGCAGCTA+TGG	0.484474	3.3:+43157352	MS.gene30177:CDS
GGATACAGCTTCAGATAGAC+AGG	0.484963	3.3:+43157374	MS.gene30177:CDS
ATGCCAATATGACGGCCATC+AGG	0.485428	3.3:-43157321	None:intergenic
TTCCTCTTCAGGAACACTTA+CGG	0.486142	3.3:+43156270	MS.gene30177:CDS
CTAGCTACTGGCGGAGGCAG+TGG	0.487153	3.3:+43157828	MS.gene30177:CDS
CACTTCAAGAGTTCTTCATA+TGG	0.487383	3.3:+43158043	MS.gene30177:CDS
TTGATTATCATTCCCTCCAC+TGG	0.491219	3.3:-43157700	None:intergenic
TTGCCACTGTCCAACCATCT+GGG	0.492660	3.3:-43158073	None:intergenic
ACTTTGGAAATATCCTTCAA+TGG	0.495344	3.3:+43157995	MS.gene30177:CDS
GATGGCCGTCATATTGGCAT+TGG	0.495923	3.3:+43157324	MS.gene30177:CDS
ATATCCTTCAATGGTGAAGA+TGG	0.498891	3.3:+43158004	MS.gene30177:CDS
CGCACTACATGGTGACTGAA+GGG	0.507766	3.3:+43156521	MS.gene30177:CDS
AAGAAATCAACTGGCGGCGT+AGG	0.510142	3.3:-43156667	None:intergenic
ATGCGGCTCAAATGTGAAAA+CGG	0.515552	3.3:-43158185	None:intergenic
GGGCATGACCGAGAAGTTTG+CGG	0.518071	3.3:+43157645	MS.gene30177:CDS
CTGCAGTTGAGAACTTTGAA+AGG	0.529944	3.3:+43157498	MS.gene30177:intron
GAACTTTGAAAGGTGGGCAT+AGG	0.530523	3.3:+43157508	MS.gene30177:CDS
AATAACCACATACTCACAAC+CGG	0.531617	3.3:+43157555	MS.gene30177:CDS
TCCCTCCGGTTGTGAGTATG+TGG	0.533027	3.3:-43157560	None:intergenic
GCCCCGGAGAATTATTCCTC+AGG	0.535877	3.3:+43156741	MS.gene30177:CDS
CTCATTCTTGTTCCACAACA+GGG	0.537252	3.3:-43157928	None:intergenic
CTGCAGTAAAAGCTCTTGCT+TGG	0.539760	3.3:+43157784	MS.gene30177:CDS
CACCTGAGGAATAATTCTCC+GGG	0.542926	3.3:-43156743	None:intergenic
TGATAATCAACTTTACATCT+GGG	0.543770	3.3:+43157713	MS.gene30177:CDS
TTTCGATTATGCGCACTACA+TGG	0.544215	3.3:+43156510	MS.gene30177:CDS
TGCTTGAACTCAGTTGACAC+TGG	0.544299	3.3:+43157891	MS.gene30177:CDS
GACAGTGGCAACAGCTGCAG+CGG	0.545471	3.3:+43158085	MS.gene30177:CDS
AGGGAGCTACTCAGCTCTCA+TGG	0.545560	3.3:+43157951	MS.gene30177:CDS
AACGGTAACAAGTTCTGAAG+TGG	0.549127	3.3:-43157259	None:intergenic
TATTTGTGGGATGCATCAAA+CGG	0.551368	3.3:+43157234	MS.gene30177:CDS
AAATCAACTGGCGGCGTAGG+TGG	0.551699	3.3:-43156664	None:intergenic
CAACTCTGAAGTGCAGCTAT+GGG	0.552386	3.3:+43157353	MS.gene30177:CDS
TAATCCAATTGCAAGAACAT+TGG	0.554610	3.3:-43157199	None:intergenic
CTCACAACCGGAGGGATGGA+TGG	0.555824	3.3:+43157567	MS.gene30177:CDS
CATATGGCGCAGAGCCCAGA+TGG	0.555918	3.3:+43158059	MS.gene30177:CDS
GTGGGCATAGGCAGCGAGTA+GGG	0.557831	3.3:+43157520	MS.gene30177:CDS
AGTTGAGAACTTTGAAAGGT+GGG	0.559397	3.3:+43157502	MS.gene30177:intron
TGGCGCAGAGCCCAGATGGT+TGG	0.562028	3.3:+43158063	MS.gene30177:CDS
TACCTCAATTTACTGGATTG+GGG	0.562441	3.3:+43157171	MS.gene30177:CDS
GCTAGTAAATTCCCTTGGAA+AGG	0.568517	3.3:-43157810	None:intergenic
GTCTACAATTGGCCAGTGGA+GGG	0.570881	3.3:+43157688	MS.gene30177:CDS
GTGAAGATGGCAGAACTCAA+TGG	0.571074	3.3:+43158017	MS.gene30177:CDS
GCGCACTACATGGTGACTGA+AGG	0.571364	3.3:+43156520	MS.gene30177:CDS
CATACTCACAACCGGAGGGA+TGG	0.571933	3.3:+43157563	MS.gene30177:CDS
CAGTTGAGAACTTTGAAAGG+TGG	0.572101	3.3:+43157501	MS.gene30177:intron
GGTCTACAATTGGCCAGTGG+AGG	0.575013	3.3:+43157687	MS.gene30177:CDS
TTCCCCAATCCAGTAAATTG+AGG	0.576674	3.3:-43157173	None:intergenic
ACTGAAGGGGCTAAAGCTAA+AGG	0.581037	3.3:+43156535	MS.gene30177:CDS
AGGGGCTAAAGCTAAAGGTA+AGG	0.581254	3.3:+43156540	MS.gene30177:CDS
TCTGTAAGTTGGGCTCCTGA+TGG	0.584539	3.3:+43157306	MS.gene30177:CDS
CCCAACTTACAGATGTGATA+GGG	0.585245	3.3:-43157296	None:intergenic
AATTCTACCATCCATCCCTC+CGG	0.585408	3.3:-43157574	None:intergenic
AAAAGCATTCCAAAACCTCA+AGG	0.587395	3.3:-43158114	None:intergenic
GGTGGGCATAGGCAGCGAGT+AGG	0.596714	3.3:+43157519	MS.gene30177:CDS
AGCGAGTAGGGTCTTTGGCA+TGG	0.601597	3.3:+43157532	MS.gene30177:CDS
TTCCTGTAAGATTCTCTGGA+AGG	0.603011	3.3:-43156583	None:intergenic
AAGATAAAACCATCAAGCCC+CGG	0.604296	3.3:+43156725	MS.gene30177:CDS
GTTACCGTTGATGATGAAGA+TGG	0.607070	3.3:+43157273	MS.gene30177:CDS
ATTGGATGCTCCAGACATTG+TGG	0.612151	3.3:+43157140	MS.gene30177:CDS
TGTTGTGGAACAAGAATGAG+AGG	0.618210	3.3:+43157931	MS.gene30177:CDS
AGGGCCATCTTCATCATCAA+CGG	0.626287	3.3:-43157277	None:intergenic
GCTTGAACTCAGTTGACACT+GGG	0.627877	3.3:+43157892	MS.gene30177:CDS
CAGTAGCTAGTAAATTCCCT+TGG	0.629888	3.3:-43157815	None:intergenic
AATTTACTAGCTACTGGCGG+AGG	0.633586	3.3:+43157822	MS.gene30177:CDS
TCAAGCCAATGCCAATATGA+CGG	0.636859	3.3:-43157329	None:intergenic
GAGCCCAGATGGTTGGACAG+TGG	0.641101	3.3:+43158070	MS.gene30177:CDS
TCTGGTCTACAATTGGCCAG+TGG	0.642557	3.3:+43157684	MS.gene30177:CDS
ACCACATACTCACAACCGGA+GGG	0.650856	3.3:+43157559	MS.gene30177:CDS
GCACTACATGGTGACTGAAG+GGG	0.656801	3.3:+43156522	MS.gene30177:CDS
AGTTTGCGGATTGAAATGGT+CGG	0.668119	3.3:+43157659	MS.gene30177:CDS
GTTCAAGCATGCACCTGTGT+GGG	0.668846	3.3:-43157877	None:intergenic
CTGCAGCGGATGAAACCTTG+AGG	0.678181	3.3:+43158099	MS.gene30177:CDS
AGTTCAAGCATGCACCTGTG+TGG	0.678713	3.3:-43157878	None:intergenic
ACGTTGTTGAAACATACAGA+GGG	0.688874	3.3:+43157625	MS.gene30177:CDS
AACCACATACTCACAACCGG+AGG	0.695066	3.3:+43157558	MS.gene30177:CDS
TGAGAGAAGAAATCAACTGG+CGG	0.700989	3.3:-43156673	None:intergenic
GGGATAGAAGTACTTCAACA+AGG	0.702662	3.3:+43157733	MS.gene30177:CDS
GGGAATTTACTAGCTACTGG+CGG	0.715639	3.3:+43157819	MS.gene30177:CDS
CACGTTGTTGAAACATACAG+AGG	0.715895	3.3:+43157624	MS.gene30177:CDS
ATGTTACTACTTATCGAATG+CGG	0.717524	3.3:-43158202	None:intergenic
ACCTGAGGAATAATTCTCCG+GGG	0.725388	3.3:-43156742	None:intergenic
GTTGTGGAACAAGAATGAGA+GGG	0.727155	3.3:+43157932	MS.gene30177:CDS
ATAGAAGTACTTCAACAAGG+TGG	0.753913	3.3:+43157736	MS.gene30177:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAGAAATTTTTTCATTATTA+GGG	+	chr3.3:43156316-43156335	MS.gene30177:intron	10.0%
!!!	TAAGAAATTTTTTCATTATT+AGG	+	chr3.3:43156315-43156334	MS.gene30177:intron	10.0%
!!!	ATATTTTATCATTATCGATT+TGG	+	chr3.3:43156815-43156834	MS.gene30177:intron	15.0%
!!!	CAAGTACAAAATTTTAAAAT+CGG	-	chr3.3:43156886-43156905	None:intergenic	15.0%
!!	AATATTAGAAAACTAGTAGT+AGG	-	chr3.3:43157463-43157482	None:intergenic	20.0%
!!!	ATAATAATGTATCGATTGAA+GGG	-	chr3.3:43157420-43157439	None:intergenic	20.0%
!!!	ATTAGTTTTTGAAAATCAAG+TGG	+	chr3.3:43156906-43156925	MS.gene30177:intron	20.0%
!!!	CACAAAATTGCAAAATATTT+TGG	-	chr3.3:43157079-43157098	None:intergenic	20.0%
!!!	TAAATGAACTCTAGTTTAAA+CGG	+	chr3.3:43156853-43156872	MS.gene30177:intron	20.0%
!!!	TTAGTTTTTGAAAATCAAGT+GGG	+	chr3.3:43156907-43156926	MS.gene30177:intron	20.0%
!	AAATCCATAACTGATCTATT+CGG	-	chr3.3:43156493-43156512	None:intergenic	25.0%
!	ATGATAATCAACTTTACATC+TGG	+	chr3.3:43157712-43157731	MS.gene30177:CDS	25.0%
!	TGATAATCAACTTTACATCT+GGG	+	chr3.3:43157713-43157732	MS.gene30177:CDS	25.0%
!!	GATTTGAGAATTTTGAACTT+CGG	+	chr3.3:43157014-43157033	MS.gene30177:intron	25.0%
!!!	CATAATAATGTATCGATTGA+AGG	-	chr3.3:43157421-43157440	None:intergenic	25.0%
	AATGTTCTTGCAATTGGATT+AGG	+	chr3.3:43157201-43157220	MS.gene30177:CDS	30.0%
	AGAAAACTAGTAGTAGGAAA+TGG	-	chr3.3:43157457-43157476	None:intergenic	30.0%
	AGGAAACACATTGTATTTGT+GGG	+	chr3.3:43157221-43157240	MS.gene30177:CDS	30.0%
	ATTACCTCAATTTACTGGAT+TGG	+	chr3.3:43157169-43157188	MS.gene30177:CDS	30.0%
	ATTATTTGTTTGTGACCAGT+TGG	+	chr3.3:43156456-43156475	MS.gene30177:intron	30.0%
	CTATGTCATGATAACTAACT+AGG	+	chr3.3:43156388-43156407	MS.gene30177:intron	30.0%
	TAATCCAATTGCAAGAACAT+TGG	-	chr3.3:43157202-43157221	None:intergenic	30.0%
	TAGGAAACACATTGTATTTG+TGG	+	chr3.3:43157220-43157239	MS.gene30177:CDS	30.0%
	TGACTATTACCTCAATTTAC+TGG	+	chr3.3:43157164-43157183	MS.gene30177:CDS	30.0%
	TTACCTCAATTTACTGGATT+GGG	+	chr3.3:43157170-43157189	MS.gene30177:CDS	30.0%
!	ACTTTGGAAATATCCTTCAA+TGG	+	chr3.3:43157995-43158014	MS.gene30177:CDS	30.0%
!	TGTTTTCGTAATCGTTTGTA+TGG	+	chr3.3:43156937-43156956	MS.gene30177:intron	30.0%
!!	AAAACGTGTTTGAGTTTTGA+TGG	-	chr3.3:43156252-43156271	None:intergenic	30.0%
!!	AGACAGGTTTTATACTTATC+TGG	+	chr3.3:43157390-43157409	MS.gene30177:intron	30.0%
!!	TCTTATCTGAAAGTGTTGTA+TGG	+	chr3.3:43157047-43157066	MS.gene30177:intron	30.0%
!!	TCTTGTGCTAATTGATTTGT+TGG	+	chr3.3:43156985-43157004	MS.gene30177:intron	30.0%
	AATAACCACATACTCACAAC+CGG	+	chr3.3:43157555-43157574	MS.gene30177:CDS	35.0%
	ACGTTGTTGAAACATACAGA+GGG	+	chr3.3:43157625-43157644	MS.gene30177:CDS	35.0%
	ATATCCTTCAATGGTGAAGA+TGG	+	chr3.3:43158004-43158023	MS.gene30177:CDS	35.0%
	ATTTCAATCCGCAAACTTCT+CGG	-	chr3.3:43157656-43157675	None:intergenic	35.0%
	CACTTCAAGAGTTCTTCATA+TGG	+	chr3.3:43158043-43158062	MS.gene30177:CDS	35.0%
	CATACCGAATAGATCAGTTA+TGG	+	chr3.3:43156486-43156505	MS.gene30177:CDS	35.0%
	GTTCTTGAAAGCTAGAATTC+TGG	-	chr3.3:43156641-43156660	None:intergenic	35.0%
	TAATAGTCATCCACAATGTC+TGG	-	chr3.3:43157153-43157172	None:intergenic	35.0%
	TACCTCAATTTACTGGATTG+GGG	+	chr3.3:43157171-43157190	MS.gene30177:CDS	35.0%
	TATTTGTGGGATGCATCAAA+CGG	+	chr3.3:43157234-43157253	MS.gene30177:CDS	35.0%
	TCATGAGAGAAGAAATCAAC+TGG	-	chr3.3:43156679-43156698	None:intergenic	35.0%
!	AGTTGAGAACTTTGAAAGGT+GGG	+	chr3.3:43157502-43157521	MS.gene30177:intron	35.0%
!!	AAAAGCATTCCAAAACCTCA+AGG	-	chr3.3:43158117-43158136	None:intergenic	35.0%
!!	ATAGAAGTACTTCAACAAGG+TGG	+	chr3.3:43157736-43157755	MS.gene30177:CDS	35.0%
!!	CAGAAGCTTTAAACATGAAC+AGG	+	chr3.3:43156614-43156633	MS.gene30177:CDS	35.0%
!!!	TTGAGGTTTTGGAATGCTTT+TGG	+	chr3.3:43158116-43158135	MS.gene30177:CDS	35.0%
	AAGATAAAACCATCAAGCCC+CGG	+	chr3.3:43156725-43156744	MS.gene30177:CDS	40.0%
	ACACCTGAGGAATAATTCTC+CGG	-	chr3.3:43156747-43156766	None:intergenic	40.0%
	ATGCGGCTCAAATGTGAAAA+CGG	-	chr3.3:43158188-43158207	None:intergenic	40.0%
	CAAGGGAATTTACTAGCTAC+TGG	+	chr3.3:43157816-43157835	MS.gene30177:CDS	40.0%
	CACAGAATCAGCTAACACTT+TGG	+	chr3.3:43157979-43157998	MS.gene30177:CDS	40.0%
	CACGTTGTTGAAACATACAG+AGG	+	chr3.3:43157624-43157643	MS.gene30177:CDS	40.0%
	CAGTAGCTAGTAAATTCCCT+TGG	-	chr3.3:43157818-43157837	None:intergenic	40.0%
	CCCAACTTACAGATGTGATA+GGG	-	chr3.3:43157299-43157318	None:intergenic	40.0%
	CCCTATCACATCTGTAAGTT+GGG	+	chr3.3:43157296-43157315	MS.gene30177:CDS	40.0%
	CTCATTCTTGTTCCACAACA+GGG	-	chr3.3:43157931-43157950	None:intergenic	40.0%
	CTGCAGTTGAGAACTTTGAA+AGG	+	chr3.3:43157498-43157517	MS.gene30177:intron	40.0%
	GCTAGTAAATTCCCTTGGAA+AGG	-	chr3.3:43157813-43157832	None:intergenic	40.0%
	GTGGGTATTCCAAAACTTGA+TGG	-	chr3.3:43157862-43157881	None:intergenic	40.0%
	GTTGTGGAACAAGAATGAGA+GGG	+	chr3.3:43157932-43157951	MS.gene30177:CDS	40.0%
	TAGCTTCCTGTAAGATTCTC+TGG	-	chr3.3:43156590-43156609	None:intergenic	40.0%
	TCAAGCCAATGCCAATATGA+CGG	-	chr3.3:43157332-43157351	None:intergenic	40.0%
	TCGGTATGAATCTATCCAAC+TGG	-	chr3.3:43156474-43156493	None:intergenic	40.0%
	TCTCATTCTTGTTCCACAAC+AGG	-	chr3.3:43157932-43157951	None:intergenic	40.0%
	TGAGAGAAGAAATCAACTGG+CGG	-	chr3.3:43156676-43156695	None:intergenic	40.0%
	TGTTGTGGAACAAGAATGAG+AGG	+	chr3.3:43157931-43157950	MS.gene30177:CDS	40.0%
	TTCCCCAATCCAGTAAATTG+AGG	-	chr3.3:43157176-43157195	None:intergenic	40.0%
	TTCCTCTTCAGGAACACTTA+CGG	+	chr3.3:43156270-43156289	MS.gene30177:CDS	40.0%
	TTCCTGTAAGATTCTCTGGA+AGG	-	chr3.3:43156586-43156605	None:intergenic	40.0%
	TTGATTATCATTCCCTCCAC+TGG	-	chr3.3:43157703-43157722	None:intergenic	40.0%
	TTTCGATTATGCGCACTACA+TGG	+	chr3.3:43156510-43156529	MS.gene30177:CDS	40.0%
!	AGTTTGCGGATTGAAATGGT+CGG	+	chr3.3:43157659-43157678	MS.gene30177:CDS	40.0%
!	CAGTTGAGAACTTTGAAAGG+TGG	+	chr3.3:43157501-43157520	MS.gene30177:intron	40.0%
!	GAGAAGTTTGCGGATTGAAA+TGG	+	chr3.3:43157655-43157674	MS.gene30177:CDS	40.0%
!	GCGATGAAACCATCAAGTTT+TGG	+	chr3.3:43157850-43157869	MS.gene30177:CDS	40.0%
!	TCAAACACGTTTTCCTCTTC+AGG	+	chr3.3:43156259-43156278	MS.gene30177:CDS	40.0%
!!	AACGGTAACAAGTTCTGAAG+TGG	-	chr3.3:43157262-43157281	None:intergenic	40.0%
!!	ACGTGTTTGAGTTTTGATGG+CGG	-	chr3.3:43156249-43156268	None:intergenic	40.0%
!!	GGGATAGAAGTACTTCAACA+AGG	+	chr3.3:43157733-43157752	MS.gene30177:CDS	40.0%
!!	GTTACCGTTGATGATGAAGA+TGG	+	chr3.3:43157273-43157292	MS.gene30177:CDS	40.0%
!!	TTGTTTTGCAGACTTCTGAG+AGG	+	chr3.3:43157115-43157134	MS.gene30177:intron	40.0%
!!!	AAGTTTTGGAATACCCACAC+AGG	+	chr3.3:43157864-43157883	MS.gene30177:CDS	40.0%
	AATAATTCTCCGGGGCTTGA+TGG	-	chr3.3:43156737-43156756	None:intergenic	45.0%
	AATTCTACCATCCATCCCTC+CGG	-	chr3.3:43157577-43157596	None:intergenic	45.0%
	AATTTACTAGCTACTGGCGG+AGG	+	chr3.3:43157822-43157841	MS.gene30177:CDS	45.0%
	ACCTGAGGAATAATTCTCCG+GGG	-	chr3.3:43156745-43156764	None:intergenic	45.0%
	ACTGAAGGGGCTAAAGCTAA+AGG	+	chr3.3:43156535-43156554	MS.gene30177:CDS	45.0%
	AGCGCCAATGTTCTTGCAAT+TGG	+	chr3.3:43157195-43157214	MS.gene30177:CDS	45.0%
	AGGGCCATCTTCATCATCAA+CGG	-	chr3.3:43157280-43157299	None:intergenic	45.0%
	AGGGGCTAAAGCTAAAGGTA+AGG	+	chr3.3:43156540-43156559	MS.gene30177:CDS	45.0%
	ATTGGATGCTCCAGACATTG+TGG	+	chr3.3:43157140-43157159	MS.gene30177:CDS	45.0%
	CACCTGAGGAATAATTCTCC+GGG	-	chr3.3:43156746-43156765	None:intergenic	45.0%
	CTCCTTCCAGAGAATCTTAC+AGG	+	chr3.3:43156581-43156600	MS.gene30177:CDS	45.0%
	CTGCAGTAAAAGCTCTTGCT+TGG	+	chr3.3:43157784-43157803	MS.gene30177:CDS	45.0%
	GCCCAACTTACAGATGTGAT+AGG	-	chr3.3:43157300-43157319	None:intergenic	45.0%
	GCCCTATCACATCTGTAAGT+TGG	+	chr3.3:43157295-43157314	MS.gene30177:CDS	45.0%
	GCTTGAACTCAGTTGACACT+GGG	+	chr3.3:43157892-43157911	MS.gene30177:CDS	45.0%
	GGATACAGCTTCAGATAGAC+AGG	+	chr3.3:43157374-43157393	MS.gene30177:CDS	45.0%
	GGGAATTTACTAGCTACTGG+CGG	+	chr3.3:43157819-43157838	MS.gene30177:CDS	45.0%
	GTGAAGATGGCAGAACTCAA+TGG	+	chr3.3:43158017-43158036	MS.gene30177:CDS	45.0%
	TCTGCCATCTTCACCATTGA+AGG	-	chr3.3:43158011-43158030	None:intergenic	45.0%
	TGCTTGAACTCAGTTGACAC+TGG	+	chr3.3:43157891-43157910	MS.gene30177:CDS	45.0%
!	AACGGCTCAACTCTTGCTTT+TGG	-	chr3.3:43158170-43158189	None:intergenic	45.0%
!	CGGATGAAACCTTGAGGTTT+TGG	+	chr3.3:43158105-43158124	MS.gene30177:CDS	45.0%
!	GAACTTTGAAAGGTGGGCAT+AGG	+	chr3.3:43157508-43157527	MS.gene30177:CDS	45.0%
!	GCAGACTTCTGAGAGGATAT+TGG	+	chr3.3:43157122-43157141	MS.gene30177:intron	45.0%
!!	ACAACTCTGAAGTGCAGCTA+TGG	+	chr3.3:43157352-43157371	MS.gene30177:CDS	45.0%
!!	CAACTCTGAAGTGCAGCTAT+GGG	+	chr3.3:43157353-43157372	MS.gene30177:CDS	45.0%
!!	GCAGCTTTTGTTGCTACTTC+TGG	-	chr3.3:43158146-43158165	None:intergenic	45.0%
!!	TTGCTTGGTGTCCTTTCCAA+GGG	+	chr3.3:43157799-43157818	MS.gene30177:CDS	45.0%
	AACCACATACTCACAACCGG+AGG	+	chr3.3:43157558-43157577	MS.gene30177:CDS	50.0%
	AAGAAATCAACTGGCGGCGT+AGG	-	chr3.3:43156670-43156689	None:intergenic	50.0%
	ACCACATACTCACAACCGGA+GGG	+	chr3.3:43157559-43157578	MS.gene30177:CDS	50.0%
	AGTTCAAGCATGCACCTGTG+TGG	-	chr3.3:43157881-43157900	None:intergenic	50.0%
	ATGCCAATATGACGGCCATC+AGG	-	chr3.3:43157324-43157343	None:intergenic	50.0%
	CGCACTACATGGTGACTGAA+GGG	+	chr3.3:43156521-43156540	MS.gene30177:CDS	50.0%
	CTCAGGTATGTTCCCTGTTG+TGG	+	chr3.3:43157916-43157935	MS.gene30177:CDS	50.0%
	CTCCGTAAGTGTTCCTGAAG+AGG	-	chr3.3:43156275-43156294	None:intergenic	50.0%
	CTTCAACAAGGTGGCTTCAC+AGG	+	chr3.3:43157745-43157764	MS.gene30177:CDS	50.0%
	GATGGCCGTCATATTGGCAT+TGG	+	chr3.3:43157324-43157343	MS.gene30177:CDS	50.0%
	GCACTACATGGTGACTGAAG+GGG	+	chr3.3:43156522-43156541	MS.gene30177:CDS	50.0%
	GTCTACAATTGGCCAGTGGA+GGG	+	chr3.3:43157688-43157707	MS.gene30177:CDS	50.0%
	GTTCAAGCATGCACCTGTGT+GGG	-	chr3.3:43157880-43157899	None:intergenic	50.0%
	TCTGGTCTACAATTGGCCAG+TGG	+	chr3.3:43157684-43157703	MS.gene30177:CDS	50.0%
	TTGCCACTGTCCAACCATCT+GGG	-	chr3.3:43158076-43158095	None:intergenic	50.0%
!	GGATTGAAATGGTCGGCTTC+TGG	+	chr3.3:43157666-43157685	MS.gene30177:CDS	50.0%
!	GTCGGCTTCTGGTCTACAAT+TGG	+	chr3.3:43157677-43157696	MS.gene30177:CDS	50.0%
!	TCTGTAAGTTGGGCTCCTGA+TGG	+	chr3.3:43157306-43157325	MS.gene30177:CDS	50.0%
!	TGGAAGGAGAGCACACTTCT+GGG	-	chr3.3:43156570-43156589	None:intergenic	50.0%
!!	CTTGCTTGGTGTCCTTTCCA+AGG	+	chr3.3:43157798-43157817	MS.gene30177:CDS	50.0%
	AAATCAACTGGCGGCGTAGG+TGG	-	chr3.3:43156667-43156686	None:intergenic	55.0%
	AGCGAGTAGGGTCTTTGGCA+TGG	+	chr3.3:43157532-43157551	MS.gene30177:CDS	55.0%
	AGGGAGCTACTCAGCTCTCA+TGG	+	chr3.3:43157951-43157970	MS.gene30177:CDS	55.0%
	CACACACACACACACACCTG+AGG	-	chr3.3:43156760-43156779	None:intergenic	55.0%
	CATACTCACAACCGGAGGGA+TGG	+	chr3.3:43157563-43157582	MS.gene30177:CDS	55.0%
	CTCAGTTGACACTGGGTCTC+AGG	+	chr3.3:43157899-43157918	MS.gene30177:CDS	55.0%
	CTGCAGCGGATGAAACCTTG+AGG	+	chr3.3:43158099-43158118	MS.gene30177:CDS	55.0%
	GCCCCGGAGAATTATTCCTC+AGG	+	chr3.3:43156741-43156760	MS.gene30177:CDS	55.0%
	GCGCACTACATGGTGACTGA+AGG	+	chr3.3:43156520-43156539	MS.gene30177:CDS	55.0%
	GCTCCTGATGGCCGTCATAT+TGG	+	chr3.3:43157318-43157337	MS.gene30177:CDS	55.0%
	GGGCATGACCGAGAAGTTTG+CGG	+	chr3.3:43157645-43157664	MS.gene30177:CDS	55.0%
	GGTCTACAATTGGCCAGTGG+AGG	+	chr3.3:43157687-43157706	MS.gene30177:CDS	55.0%
	GTTGCCACTGTCCAACCATC+TGG	-	chr3.3:43158077-43158096	None:intergenic	55.0%
	TAGGCAGCGAGTAGGGTCTT+TGG	+	chr3.3:43157527-43157546	MS.gene30177:CDS	55.0%
	TCCCTCCGGTTGTGAGTATG+TGG	-	chr3.3:43157563-43157582	None:intergenic	55.0%
!	CTGGAAGGAGAGCACACTTC+TGG	-	chr3.3:43156571-43156590	None:intergenic	55.0%
	CATATGGCGCAGAGCCCAGA+TGG	+	chr3.3:43158059-43158078	MS.gene30177:CDS	60.0%
	CTCACAACCGGAGGGATGGA+TGG	+	chr3.3:43157567-43157586	MS.gene30177:CDS	60.0%
	GACAGTGGCAACAGCTGCAG+CGG	+	chr3.3:43158085-43158104	MS.gene30177:CDS	60.0%
	GAGCCCAGATGGTTGGACAG+TGG	+	chr3.3:43158070-43158089	MS.gene30177:CDS	60.0%
	GTGGGCATAGGCAGCGAGTA+GGG	+	chr3.3:43157520-43157539	MS.gene30177:CDS	60.0%
	CTAGCTACTGGCGGAGGCAG+TGG	+	chr3.3:43157828-43157847	MS.gene30177:CDS	65.0%
	GGTGGGCATAGGCAGCGAGT+AGG	+	chr3.3:43157519-43157538	MS.gene30177:CDS	65.0%
	TGGCGCAGAGCCCAGATGGT+TGG	+	chr3.3:43158063-43158082	MS.gene30177:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	43156221	43158214	43156221	ID=MS.gene30177
chr3.3	mRNA	43156221	43158214	43156221	ID=MS.gene30177.t1;Parent=MS.gene30177
chr3.3	exon	43156221	43156313	43156221	ID=MS.gene30177.t1.exon1;Parent=MS.gene30177.t1
chr3.3	CDS	43156221	43156313	43156221	ID=cds.MS.gene30177.t1;Parent=MS.gene30177.t1
chr3.3	exon	43156475	43156762	43156475	ID=MS.gene30177.t1.exon2;Parent=MS.gene30177.t1
chr3.3	CDS	43156475	43156762	43156475	ID=cds.MS.gene30177.t1;Parent=MS.gene30177.t1
chr3.3	exon	43157126	43157395	43157126	ID=MS.gene30177.t1.exon3;Parent=MS.gene30177.t1
chr3.3	CDS	43157126	43157395	43157126	ID=cds.MS.gene30177.t1;Parent=MS.gene30177.t1
chr3.3	exon	43157504	43158214	43157504	ID=MS.gene30177.t1.exon4;Parent=MS.gene30177.t1
chr3.3	CDS	43157504	43158214	43157504	ID=cds.MS.gene30177.t1;Parent=MS.gene30177.t1

Gene Sequence

>MS.gene30177

ATGGCTTCAGGTTCTTTGAGTTCTTCCGCCATCAAAACTCAAACACGTTTTCCTCTTCAGGAACACTTACGGAGAAAGACTTCAAATGAAAATGTAAGAAATTTTTTCATTATTAGGGTTTCAAACTTTCAATGTAATTCTGTTTTGAAAAAGAATGAATTTTGAAACTATGTCATGATAACTAACTAGGTACTTTATTGTGAGAATTTCTTTATAAAAATTAAGTAGTTTATTAATTATTTGTTTGTGACCAGTTGGATAGATTCATACCGAATAGATCAGTTATGGATTTCGATTATGCGCACTACATGGTGACTGAAGGGGCTAAAGCTAAAGGTAAGGAAAACCCAGAAGTGTGCTCTCCTTCCAGAGAATCTTACAGGAAGCTACTTGCAGAAGCTTTAAACATGAACAGGACCAGAATTCTAGCTTTCAAGAACAAGCCACCTACGCCGCCAGTTGATTTCTTCTCTCATGATATCATTACTTCATCAACTCTTCGACAAGATAAAACCATCAAGCCCCGGAGAATTATTCCTCAGGTGTGTGTGTGTGTGTGTGTGTATTTCTTAATGATATCTATTCATATTTCATATATTTTATCATTATCGATTTGGTTGCATGAACTCGTTTAAATGAACTCTAGTTTAAACGGCAAAAGTCCGATTTTAAAATTTTGTACTTGATTAGTTTTTGAAAATCAAGTGGGTCTGAGTTGTTTTCGTAATCGTTTGTATGGTGTGTGTAATCACAATAGTTTGCAATCTTGTGCTAATTGATTTGTTGGTTATCTGATTTGAGAATTTTGAACTTCGGTTTTCTTAATTCTTATCTGAAAGTGTTGTATGGTGTAAACCAAAATATTTTGCAATTTTGTGCTAATTGATTGATTTGTTGTTTTGCAGACTTCTGAGAGGATATTGGATGCTCCAGACATTGTGGATGACTATTACCTCAATTTACTGGATTGGGGAAGCGCCAATGTTCTTGCAATTGGATTAGGAAACACATTGTATTTGTGGGATGCATCAAACGGTTCCACTTCAGAACTTGTTACCGTTGATGATGAAGATGGCCCTATCACATCTGTAAGTTGGGCTCCTGATGGCCGTCATATTGGCATTGGCTTGAACAACTCTGAAGTGCAGCTATGGGATACAGCTTCAGATAGACAGGTTTTATACTTATCTGGTTTACCCTTCAATCGATACATTATTATGAGCTATTCAGTATCCATTTCCTACTACTAGTTTTCTAATATTATTCATGTTATGTTTCTGCAGTTGAGAACTTTGAAAGGTGGGCATAGGCAGCGAGTAGGGTCTTTGGCATGGAATAACCACATACTCACAACCGGAGGGATGGATGGTAGAATTATCAATAATGATGTAAGAATTAGAGCACACGTTGTTGAAACATACAGAGGGCATGACCGAGAAGTTTGCGGATTGAAATGGTCGGCTTCTGGTCTACAATTGGCCAGTGGAGGGAATGATAATCAACTTTACATCTGGGATAGAAGTACTTCAACAAGGTGGCTTCACAGGCTTGAAGATCATACATCTGCAGTAAAAGCTCTTGCTTGGTGTCCTTTCCAAGGGAATTTACTAGCTACTGGCGGAGGCAGTGGCGATGAAACCATCAAGTTTTGGAATACCCACACAGGTGCATGCTTGAACTCAGTTGACACTGGGTCTCAGGTATGTTCCCTGTTGTGGAACAAGAATGAGAGGGAGCTACTCAGCTCTCATGGTTTTACACAGAATCAGCTAACACTTTGGAAATATCCTTCAATGGTGAAGATGGCAGAACTCAATGGTCACACTTCAAGAGTTCTTCATATGGCGCAGAGCCCAGATGGTTGGACAGTGGCAACAGCTGCAGCGGATGAAACCTTGAGGTTTTGGAATGCTTTTGGCACTCCAGAAGTAGCAACAAAAGCTGCACCAAAAGCAAGAGTTGAGCCGTTTTCACATTTGAGCCGCATTCGATAA

Protein sequence

>MS.gene30177.t1

MASGSLSSSAIKTQTRFPLQEHLRRKTSNENLDRFIPNRSVMDFDYAHYMVTEGAKAKGKENPEVCSPSRESYRKLLAEALNMNRTRILAFKNKPPTPPVDFFSHDIITSSTLRQDKTIKPRRIIPQTSERILDAPDIVDDYYLNLLDWGSANVLAIGLGNTLYLWDASNGSTSELVTVDDEDGPITSVSWAPDGRHIGIGLNNSEVQLWDTASDRQLRTLKGGHRQRVGSLAWNNHILTTGGMDGRIINNDVRIRAHVVETYRGHDREVCGLKWSASGLQLASGGNDNQLYIWDRSTSTRWLHRLEDHTSAVKALAWCPFQGNLLATGGGSGDETIKFWNTHTGACLNSVDTGSQVCSLLWNKNERELLSSHGFTQNQLTLWKYPSMVKMAELNGHTSRVLHMAQSPDGWTVATAAADETLRFWNAFGTPEVATKAAPKARVEPFSHLSRIR