AlfalfaGEDB | MS.gene050457

M. sativa cultivar XinJiangDaYe / MS.gene050457

KEGG: K03363
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00400.35 PF12894.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050457.t1	XP_003600092.1	94.6	428	17	2	1	422	32	459	9.00E-241	842.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050457.t1	Q9SZA4	77.3	427	89	3	1	422	34	457	6.8e-197	688.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050457.t1	G7IY01	94.6	428	17	2	1	422	32	459	6.5e-241	842.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene050457	MS.gene26021	PPI
MS.gene064035	MS.gene050457	PPI
MS.gene027441	MS.gene050457	PPI
MS.gene81663	MS.gene050457	PPI
MS.gene36842	MS.gene050457	PPI
MS.gene050457	MS.gene91775	PPI
MS.gene99554	MS.gene050457	PPI
MS.gene81660	MS.gene050457	PPI
MS.gene066046	MS.gene050457	PPI
MS.gene058128	MS.gene050457	PPI
MS.gene99588	MS.gene050457	PPI
MS.gene027075	MS.gene050457	PPI
MS.gene41291	MS.gene050457	PPI
MS.gene27223	MS.gene050457	PPI
MS.gene66342	MS.gene050457	PPI
MS.gene050272	MS.gene050457	PPI
MS.gene06176	MS.gene050457	PPI
MS.gene030294	MS.gene050457	PPI
MS.gene91851	MS.gene050457	PPI
MS.gene052956	MS.gene050457	PPI
MS.gene29768	MS.gene050457	PPI
MS.gene35613	MS.gene050457	PPI
MS.gene039682	MS.gene050457	PPI
MS.gene98540	MS.gene050457	PPI
MS.gene043742	MS.gene050457	PPI
MS.gene050457	MS.gene038519	PPI
MS.gene022502	MS.gene050457	PPI
MS.gene85534	MS.gene050457	PPI
MS.gene067321	MS.gene050457	PPI
MS.gene042783	MS.gene050457	PPI
MS.gene82020	MS.gene050457	PPI
MS.gene21734	MS.gene050457	PPI
MS.gene66343	MS.gene050457	PPI
MS.gene33377	MS.gene050457	PPI
MS.gene049021	MS.gene050457	PPI
MS.gene71204	MS.gene050457	PPI
MS.gene050457	MS.gene050456	PPI
MS.gene77885	MS.gene050457	PPI
MS.gene050457	MS.gene51258	PPI
MS.gene050457	MS.gene32723	PPI
MS.gene26672	MS.gene050457	PPI
MS.gene050457	MS.gene29842	PPI
MS.gene97379	MS.gene050457	PPI
MS.gene050457	MS.gene053363	PPI
MS.gene90626	MS.gene050457	PPI
MS.gene050456	MS.gene050457	PPI
MS.gene42253	MS.gene050457	PPI
MS.gene050457	MS.gene75493	PPI
MS.gene011448	MS.gene050457	PPI
MS.gene066501	MS.gene050457	PPI
MS.gene25244	MS.gene050457	PPI
MS.gene050457	MS.gene32726	PPI
MS.gene050457	MS.gene71122	PPI
MS.gene068273	MS.gene050457	PPI
MS.gene63085	MS.gene050457	PPI
MS.gene056455	MS.gene050457	PPI
MS.gene89986	MS.gene050457	PPI
MS.gene26019	MS.gene050457	PPI
MS.gene064762	MS.gene050457	PPI
MS.gene069995	MS.gene050457	PPI
MS.gene050457	MS.gene014321	PPI
MS.gene050457	MS.gene23881	PPI
MS.gene049022	MS.gene050457	PPI
MS.gene050457	MS.gene014931	PPI
MS.gene050457	MS.gene21888	PPI
MS.gene69965	MS.gene050457	PPI
MS.gene066500	MS.gene050457	PPI
MS.gene82740	MS.gene050457	PPI
MS.gene024753	MS.gene050457	PPI
MS.gene55882	MS.gene050457	PPI
MS.gene30172	MS.gene050457	PPI
MS.gene067322	MS.gene050457	PPI
MS.gene050457	MS.gene62819	PPI
MS.gene045937	MS.gene050457	PPI
MS.gene060496	MS.gene050457	PPI
MS.gene23852	MS.gene050457	PPI
MS.gene69966	MS.gene050457	PPI
MS.gene97943	MS.gene050457	PPI
MS.gene071113	MS.gene050457	PPI
MS.gene050457	MS.gene65775	PPI
MS.gene050457	MS.gene80163	PPI
MS.gene027071	MS.gene050457	PPI
MS.gene67157	MS.gene050457	PPI
MS.gene050457	MS.gene033401	PPI
MS.gene69396	MS.gene050457	PPI
MS.gene050457	MS.gene027072	PPI
MS.gene012203	MS.gene050457	PPI
MS.gene022109	MS.gene050457	PPI
MS.gene34651	MS.gene050457	PPI
MS.gene010910	MS.gene050457	PPI
MS.gene052188	MS.gene050457	PPI
MS.gene050457	MS.gene06177	PPI
MS.gene050457	MS.gene51278	PPI
MS.gene025229	MS.gene050457	PPI
MS.gene050457	MS.gene87218	PPI
MS.gene045133	MS.gene050457	PPI
MS.gene063222	MS.gene050457	PPI
MS.gene25515	MS.gene050457	PPI
MS.gene060495	MS.gene050457	PPI
MS.gene07706	MS.gene050457	PPI
MS.gene027074	MS.gene050457	PPI
MS.gene20160	MS.gene050457	PPI
MS.gene050457	MS.gene064862	PPI
MS.gene69687	MS.gene050457	PPI
MS.gene050457	MS.gene37226	PPI
MS.gene010912	MS.gene050457	PPI
MS.gene050457	MS.gene062662	PPI
MS.gene23811	MS.gene050457	PPI
MS.gene23810	MS.gene050457	PPI
MS.gene46611	MS.gene050457	PPI
MS.gene050457	MS.gene30177	PPI
MS.gene050457	MS.gene84466	PPI
MS.gene51335	MS.gene050457	PPI
MS.gene050457	MS.gene008833	PPI
MS.gene82021	MS.gene050457	PPI
MS.gene81658	MS.gene050457	PPI
MS.gene84465	MS.gene050457	PPI
MS.gene78281	MS.gene050457	PPI
MS.gene050457	MS.gene064435	PPI
MS.gene037950	MS.gene050457	PPI
MS.gene70008	MS.gene050457	PPI
MS.gene068249	MS.gene050457	PPI
MS.gene050457	MS.gene48655	PPI
MS.gene64441	MS.gene050457	PPI
MS.gene24806	MS.gene050457	PPI
MS.gene004844	MS.gene050457	PPI
MS.gene52355	MS.gene050457	PPI
MS.gene30176	MS.gene050457	PPI
MS.gene24951	MS.gene050457	PPI
MS.gene050457	MS.gene65463	PPI
MS.gene014419	MS.gene050457	PPI
MS.gene066464	MS.gene050457	PPI
MS.gene050457	MS.gene037184	PPI
MS.gene20161	MS.gene050457	PPI
MS.gene050457	MS.gene067705	PPI
MS.gene55883	MS.gene050457	PPI
MS.gene51262	MS.gene050457	PPI
MS.gene050270	MS.gene050457	PPI
MS.gene24922	MS.gene050457	PPI
MS.gene027073	MS.gene050457	PPI
MS.gene33406	MS.gene050457	PPI
MS.gene066467	MS.gene050457	PPI
MS.gene050457	MS.gene99973	PPI
MS.gene75059	MS.gene050457	PPI
MS.gene34712	MS.gene050457	PPI
MS.gene92674	MS.gene050457	PPI
MS.gene050457	MS.gene30174	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050457.t1	MTR_3g051690	94.626	428	17	2	1	422	32	459	0.0	843
MS.gene050457.t1	MTR_5g008010	90.398	427	32	4	1	422	32	454	0.0	797
MS.gene050457.t1	MTR_7g106870	74.825	429	97	6	1	422	33	457	0.0	668
MS.gene050457.t1	MTR_7g451830	67.366	429	116	8	2	422	19	431	0.0	566
MS.gene050457.t1	MTR_7g047640	67.366	429	116	8	2	422	19	431	0.0	566
MS.gene050457.t1	MTR_7g057540	64.884	430	125	8	1	422	15	426	0.0	534
MS.gene050457.t1	MTR_7g055590	64.802	429	125	8	1	421	15	425	0.0	531
MS.gene050457.t1	MTR_7g053100	62.005	429	139	7	1	422	25	436	0.0	518
MS.gene050457.t1	MTR_7g034625	70.755	318	86	2	97	407	13	330	1.81e-166	471
MS.gene050457.t1	MTR_2g100540	50.580	431	158	12	2	422	12	397	1.48e-127	374
MS.gene050457.t1	MTR_2g100460	56.560	343	135	5	80	420	52	382	1.63e-126	371
MS.gene050457.t1	MTR_2g060710	44.282	411	207	8	2	398	26	428	2.48e-119	355
MS.gene050457.t1	MTR_3g067940	44.286	420	212	7	2	409	57	466	3.97e-116	348
MS.gene050457.t1	MTR_4g102510	43.198	419	206	7	2	401	51	456	1.04e-110	334
MS.gene050457.t1	MTR_4g415070	58.621	58	24	0	347	404	57	114	1.90e-16	80.1
MS.gene050457.t1	MTR_2g012630	27.397	219	148	8	127	342	35	245	2.76e-15	78.6
MS.gene050457.t1	MTR_1g054300	26.263	297	161	10	147	396	114	399	2.99e-15	77.8
MS.gene050457.t1	MTR_1g054300	26.119	268	170	11	134	394	235	481	2.20e-14	75.1
MS.gene050457.t1	MTR_2g012630	27.397	219	148	8	127	342	35	245	3.23e-15	78.6
MS.gene050457.t1	MTR_6g004040	28.326	233	153	10	113	339	19	243	6.90e-15	77.4
MS.gene050457.t1	MTR_5g016370	23.693	287	163	8	116	394	312	550	1.21e-14	76.3
MS.gene050457.t1	MTR_5g016350	22.997	287	179	7	110	395	294	539	5.78e-13	70.9
MS.gene050457.t1	MTR_5g016350	22.997	287	179	7	110	395	230	475	6.91e-13	70.5
MS.gene050457.t1	MTR_4g095058	24.382	283	168	11	116	395	295	534	5.37e-12	68.2
MS.gene050457.t1	MTR_4g095058	24.382	283	168	11	116	395	293	532	6.46e-12	67.8
MS.gene050457.t1	MTR_3g106080	24.351	308	191	10	128	403	326	623	9.18e-12	67.4
MS.gene050457.t1	MTR_7g086360	23.415	205	150	4	193	395	53	252	2.42e-11	65.1
MS.gene050457.t1	MTR_1g049000	39.362	94	47	3	173	257	8	100	4.80e-11	62.0
MS.gene050457.t1	MTR_4g119620	22.581	248	177	7	155	394	81	321	5.36e-11	63.9
MS.gene050457.t1	MTR_5g022690	27.011	174	120	5	139	310	266	434	8.50e-11	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050457.t1	AT4G33270	77.283	427	89	4	1	422	34	457	0.0	687
MS.gene050457.t1	AT4G33260	77.283	427	89	4	1	422	24	447	0.0	668
MS.gene050457.t1	AT4G33260	75.122	410	94	4	1	405	24	430	0.0	635
MS.gene050457.t1	AT5G27570	68.531	429	120	6	1	422	30	450	0.0	590
MS.gene050457.t1	AT5G27080	67.133	429	126	6	1	422	22	442	0.0	580
MS.gene050457.t1	AT5G26900	66.589	428	129	5	1	422	25	444	0.0	572
MS.gene050457.t1	AT5G27945	61.456	371	123	5	68	422	62	428	1.21e-156	449
MS.gene050457.t1	AT4G22910	42.822	411	216	7	2	401	62	464	8.70e-113	339
MS.gene050457.t1	AT5G13840	51.475	305	145	3	100	404	164	465	1.56e-110	333
MS.gene050457.t1	AT5G13840	51.475	305	145	3	100	404	164	465	1.56e-110	333
MS.gene050457.t1	AT4G11920	42.118	425	212	8	2	407	53	462	1.91e-110	333
MS.gene050457.t1	AT1G11160	28.155	206	131	7	119	313	16	215	4.47e-16	81.3
MS.gene050457.t1	AT1G11160	28.155	206	131	7	119	313	16	215	4.67e-16	81.3
MS.gene050457.t1	AT1G11160	28.155	206	131	7	119	313	16	215	5.53e-16	80.9
MS.gene050457.t1	AT5G08390	29.060	234	136	10	109	339	37	243	1.16e-15	79.7
MS.gene050457.t1	AT1G11160	30.247	162	105	6	154	313	18	173	6.14e-15	77.8
MS.gene050457.t1	AT1G61210	26.339	224	142	8	111	319	6	221	1.68e-14	76.3
MS.gene050457.t1	AT1G61210	26.126	222	145	8	111	319	6	221	1.81e-14	76.3
MS.gene050457.t1	AT1G61210	26.339	224	142	8	111	319	6	221	1.95e-14	76.3
MS.gene050457.t1	AT5G52820	28.226	248	154	12	155	396	244	473	4.13e-14	74.3
MS.gene050457.t1	AT5G23430	28.774	212	138	9	113	319	19	222	1.64e-13	73.2
MS.gene050457.t1	AT5G23430	28.774	212	138	9	113	319	19	222	1.64e-13	73.2
MS.gene050457.t1	AT5G23430	28.774	212	138	9	113	319	19	222	1.79e-13	72.8
MS.gene050457.t1	AT5G25150	25.749	334	209	12	104	409	305	627	3.96e-13	71.6
MS.gene050457.t1	AT5G25150	25.749	334	209	12	104	409	305	627	4.70e-13	71.6
MS.gene050457.t1	AT2G32700	25.610	246	163	11	155	394	574	805	5.98e-13	71.2
MS.gene050457.t1	AT2G32700	25.610	246	163	11	155	394	553	784	6.06e-13	71.2
MS.gene050457.t1	AT2G32700	25.610	246	163	11	155	394	555	786	6.29e-13	71.2
MS.gene050457.t1	AT2G32700	25.610	246	163	11	155	394	555	786	6.29e-13	71.2
MS.gene050457.t1	AT2G32700	25.610	246	163	11	155	394	555	786	6.29e-13	71.2
MS.gene050457.t1	AT2G32700	25.610	246	163	11	155	394	555	786	6.29e-13	71.2
MS.gene050457.t1	AT2G32700	25.610	246	163	11	155	394	555	786	6.29e-13	71.2
MS.gene050457.t1	AT5G67320	25.000	284	165	12	116	395	330	569	1.94e-12	69.3
MS.gene050457.t1	AT3G49660	23.600	250	177	8	151	394	71	312	3.93e-12	67.4
MS.gene050457.t1	AT5G13480	31.319	182	115	7	131	309	218	392	1.02e-11	67.4
MS.gene050457.t1	AT5G13480	27.391	230	148	10	155	377	114	331	1.74e-11	66.6
MS.gene050457.t1	AT2G33340	26.042	192	134	5	207	395	236	422	1.11e-11	67.0
MS.gene050457.t1	AT2G33340	26.042	192	134	5	207	395	236	422	1.11e-11	67.0
MS.gene050457.t1	AT2G41500	25.203	246	134	8	155	398	342	539	1.16e-11	67.0
MS.gene050457.t1	AT2G41500	25.203	246	134	8	155	398	342	539	1.16e-11	67.0
MS.gene050457.t1	AT5G13480	31.319	182	115	7	131	309	241	415	1.17e-11	67.0
MS.gene050457.t1	AT5G13480	27.391	230	148	10	155	377	137	354	1.72e-11	66.6
MS.gene050457.t1	AT5G13480	31.319	182	115	7	131	309	218	392	1.22e-11	67.0
MS.gene050457.t1	AT5G13480	27.391	230	148	10	155	377	114	331	2.12e-11	66.2
MS.gene050457.t1	AT1G04510	25.116	215	152	5	207	417	236	445	1.34e-11	66.6
MS.gene050457.t1	AT2G33340	26.042	192	134	5	207	395	236	422	1.37e-11	66.6
MS.gene050457.t1	AT1G04510	25.116	215	152	5	207	417	236	445	1.55e-11	66.6
MS.gene050457.t1	AT5G13480	31.319	182	115	7	131	309	230	404	1.57e-11	66.6
MS.gene050457.t1	AT5G13480	27.391	230	148	10	155	377	126	343	2.41e-11	66.2
MS.gene050457.t1	AT4G02730	26.852	216	145	9	185	395	34	241	3.89e-11	64.3
MS.gene050457.t1	AT5G56130	23.786	206	113	5	232	395	16	219	7.10e-11	63.5

Find 125 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGATGAAACCTTGAGGTTT+TGG	0.193519	3.2:-44256059	MS.gene050457:CDS
TGACTATTACCTCAATTTAC+TGG	0.257532	3.2:-44257000	MS.gene050457:CDS
GTTGCCACTGTCCAACCATC+TGG	0.273358	3.2:+44256090	None:intergenic
AACGGCTCAGCTCTTGCTTT+TGG	0.277205	3.2:+44255997	None:intergenic
TTACCTCAATTTACTGGATT+GGG	0.283267	3.2:-44256994	MS.gene050457:CDS
AATGTTCTTGCAATTGGATT+AGG	0.295419	3.2:-44256963	MS.gene050457:CDS
CTCCTTCCAGAGAAGCTTAC+AGG	0.301895	3.2:-44257675	MS.gene050457:CDS
AGCGCCAATGTTCTTGCAAT+TGG	0.327966	3.2:-44256969	MS.gene050457:CDS
GTTCTTGAAAGCTAGAATTC+TGG	0.331154	3.2:+44257618	None:intergenic
GCAGACTTCTGAGAGGATAT+TGG	0.341248	3.2:-44257042	MS.gene050457:intron
GGATTGAAATGGTCGGCTTC+TGG	0.343844	3.2:-44256498	MS.gene050457:CDS
ATGATAATCAACTTTACATC+TGG	0.350065	3.2:-44256452	MS.gene050457:CDS
GCGATGAAACCATCAAGTTT+TGG	0.364930	3.2:-44256314	MS.gene050457:CDS
ATTACCTCAATTTACTGGAT+TGG	0.371678	3.2:-44256995	MS.gene050457:CDS
TAGCTTCCTGTAAGCTTCTC+TGG	0.375997	3.2:+44257669	None:intergenic
TCTCATTCTTGTTCCACAAC+AGG	0.376647	3.2:+44256235	None:intergenic
GCTCCTGATGGCCGTCATAT+TGG	0.389395	3.2:-44256846	MS.gene050457:CDS
ATTTCAATCCGCAAACTTCT+CGG	0.394694	3.2:+44256511	None:intergenic
AATAATTCGCCGGGGCTTGA+TGG	0.396021	3.2:+44257522	None:intergenic
CCCTATCACATCTGTAAGTT+GGG	0.396604	3.2:-44256868	MS.gene050457:CDS
TCTGCCATCTTCACCATTGA+AGG	0.397901	3.2:+44256156	None:intergenic
CTCAGTTGACACTGGGTCTC+AGG	0.408962	3.2:-44256265	MS.gene050457:CDS
TAGGAAACACATTGTATTTG+TGG	0.412235	3.2:-44256944	MS.gene050457:CDS
CAAGGGAATTTACTAGCTAC+TGG	0.417964	3.2:-44256348	MS.gene050457:CDS
GCCCTATCACATCTGTAAGT+TGG	0.420766	3.2:-44256869	MS.gene050457:CDS
GAGAAGTTTGCGGATTGAAA+TGG	0.425267	3.2:-44256509	MS.gene050457:CDS
CTCAGGTATGTTCCCTGTTG+TGG	0.426699	3.2:-44256248	MS.gene050457:CDS
TAATAGTCATCCACAATGTC+TGG	0.428065	3.2:+44257014	None:intergenic
CTTGCTTGGTGTCCTTTCCA+AGG	0.428743	3.2:-44256366	MS.gene050457:CDS
GTGGGTATTCCAAAACTTGA+TGG	0.428833	3.2:+44256305	None:intergenic
TAGGCAGCGAGTAGGGTCTT+TGG	0.428851	3.2:-44256637	MS.gene050457:CDS
CAGAAGCTCTAAACATGAAC+AGG	0.436496	3.2:-44257642	MS.gene050457:CDS
GTCGGCTTCTGGTCTACAAT+TGG	0.444050	3.2:-44256487	MS.gene050457:CDS
AAATCCATAGCTGATCTATT+CGG	0.447023	3.2:+44257766	None:intergenic
GCCCAACTTACAGATGTGAT+AGG	0.450468	3.2:+44256867	None:intergenic
AGGAAACACATTGTATTTGT+GGG	0.452538	3.2:-44256943	MS.gene050457:CDS
CTTCAACAAGGTGGCTTCAC+AGG	0.460645	3.2:-44256419	MS.gene050457:CDS
TTGCTTGGTGTCCTTTCCAA+GGG	0.471233	3.2:-44256365	MS.gene050457:CDS
CACAGAATCAGATAACACTT+TGG	0.480456	3.2:-44256185	MS.gene050457:CDS
TCATGAGAGAAGAAATCAAC+TGG	0.484334	3.2:+44257580	None:intergenic
ACAACTCTGAAGTGCAGCTA+TGG	0.484474	3.2:-44256812	MS.gene050457:CDS
GGATACAGCTTCAGATAGAC+AGG	0.484963	3.2:-44256790	MS.gene050457:intron
ATGCCAATATGACGGCCATC+AGG	0.485428	3.2:+44256843	None:intergenic
CTAGCTACTGGCGGAGGCAG+TGG	0.487153	3.2:-44256336	MS.gene050457:CDS
CACTTCAAGAGTTCTTCATA+TGG	0.487383	3.2:-44256121	MS.gene050457:CDS
TTGATTATCATTCCCTCCAC+TGG	0.491219	3.2:+44256464	None:intergenic
TTGCCACTGTCCAACCATCT+GGG	0.492660	3.2:+44256091	None:intergenic
TGGAAGGAGAGCACACTTGT+GGG	0.493184	3.2:+44257689	None:intergenic
ACTTTGGAAATATCCTTCAA+TGG	0.495344	3.2:-44256169	MS.gene050457:CDS
GATGGCCGTCATATTGGCAT+TGG	0.495923	3.2:-44256840	MS.gene050457:CDS
ATATCCTTCAATGGTGAAGA+TGG	0.498891	3.2:-44256160	MS.gene050457:CDS
CGCACTACATGGTGACTGAA+GGG	0.507766	3.2:-44257735	MS.gene050457:CDS
CTGGAAGGAGAGCACACTTG+TGG	0.508404	3.2:+44257688	None:intergenic
AAGAAATCAACTGGCGGCGT+AGG	0.510142	3.2:+44257589	None:intergenic
ATGCGGCTCAAATGTGAAAA+CGG	0.515552	3.2:+44255979	None:intergenic
GGGCATGACCGAGAAGTTTG+CGG	0.518071	3.2:-44256519	MS.gene050457:CDS
AACAGTAACAAGTTCTGAAG+TGG	0.523430	3.2:+44256905	None:intergenic
CTGCAGTTGAGAACTTTGAA+AGG	0.529944	3.2:-44256666	MS.gene050457:intron
GAACTTTGAAAGGTGGGCAT+AGG	0.530523	3.2:-44256656	MS.gene050457:CDS
AATAACCACATACTCACAAC+CGG	0.531617	3.2:-44256609	MS.gene050457:CDS
TCCCTCCGGTTGTGAGTATG+TGG	0.533027	3.2:+44256604	None:intergenic
GCCCCGGCGAATTATTCCTC+AGG	0.533857	3.2:-44257515	MS.gene050457:intron
CTCATTCTTGTTCCACAACA+GGG	0.537252	3.2:+44256236	None:intergenic
CTGCAGTAAAAGCTCTTGCT+TGG	0.539760	3.2:-44256380	MS.gene050457:CDS
ACACCTGAGGAATAATTCGC+CGG	0.542147	3.2:+44257512	None:intergenic
TGATAATCAACTTTACATCT+GGG	0.543770	3.2:-44256451	MS.gene050457:CDS
TTTCGATTATGCGCACTACA+TGG	0.544215	3.2:-44257746	MS.gene050457:CDS
TGCTTGAACTCAGTTGACAC+TGG	0.544299	3.2:-44256273	MS.gene050457:CDS
GACAGTGGCAACAGCTGCAG+CGG	0.545471	3.2:-44256079	MS.gene050457:CDS
AGGGAGCTACTCAGCTCTCA+TGG	0.545560	3.2:-44256213	MS.gene050457:CDS
TATTTGTGGGATGCATCAAA+CGG	0.551368	3.2:-44256930	MS.gene050457:CDS
AAATCAACTGGCGGCGTAGG+TGG	0.551699	3.2:+44257592	None:intergenic
CAACTCTGAAGTGCAGCTAT+GGG	0.552386	3.2:-44256811	MS.gene050457:CDS
TAATCCAATTGCAAGAACAT+TGG	0.554610	3.2:+44256965	None:intergenic
CTCACAACCGGAGGGATGGA+TGG	0.555824	3.2:-44256597	MS.gene050457:CDS
CATATGGCGCAGAGCCCAGA+TGG	0.555918	3.2:-44256105	MS.gene050457:CDS
GTGGGCATAGGCAGCGAGTA+GGG	0.557831	3.2:-44256644	MS.gene050457:CDS
CACCTGAGGAATAATTCGCC+GGG	0.559324	3.2:+44257513	None:intergenic
AGTTGAGAACTTTGAAAGGT+GGG	0.559397	3.2:-44256662	MS.gene050457:CDS
TGGCGCAGAGCCCAGATGGT+TGG	0.562028	3.2:-44256101	MS.gene050457:CDS
TACCTCAATTTACTGGATTG+GGG	0.562441	3.2:-44256993	MS.gene050457:CDS
GCTAGTAAATTCCCTTGGAA+AGG	0.568517	3.2:+44256354	None:intergenic
GTCTACAATTGGCCAGTGGA+GGG	0.570881	3.2:-44256476	MS.gene050457:CDS
GTGAAGATGGCAGAACTCAA+TGG	0.571074	3.2:-44256147	MS.gene050457:CDS
GCGCACTACATGGTGACTGA+AGG	0.571364	3.2:-44257736	MS.gene050457:CDS
CATACTCACAACCGGAGGGA+TGG	0.571933	3.2:-44256601	MS.gene050457:CDS
CAGTTGAGAACTTTGAAAGG+TGG	0.572101	3.2:-44256663	MS.gene050457:CDS
GGTCTACAATTGGCCAGTGG+AGG	0.575013	3.2:-44256477	MS.gene050457:CDS
GTTACTGTTGATGATGAAGA+TGG	0.576596	3.2:-44256891	MS.gene050457:CDS
TTCCCCAATCCAGTAAATTG+AGG	0.576674	3.2:+44256991	None:intergenic
TTCCTGTAAGCTTCTCTGGA+AGG	0.579286	3.2:+44257673	None:intergenic
ACTGAAGGGGCTAAAGCTAA+AGG	0.581037	3.2:-44257721	MS.gene050457:CDS
AGGGGCTAAAGCTAAAGGTA+AGG	0.581254	3.2:-44257716	MS.gene050457:CDS
TCTGTAAGTTGGGCTCCTGA+TGG	0.584539	3.2:-44256858	MS.gene050457:CDS
CCCAACTTACAGATGTGATA+GGG	0.585245	3.2:+44256868	None:intergenic
AATTCTACCATCCATCCCTC+CGG	0.585408	3.2:+44256590	None:intergenic
AAAAGCATTCCAAAACCTCA+AGG	0.587395	3.2:+44256050	None:intergenic
GGTGGGCATAGGCAGCGAGT+AGG	0.596714	3.2:-44256645	MS.gene050457:CDS
CATACCGAATAGATCAGCTA+TGG	0.601151	3.2:-44257770	MS.gene050457:CDS
AGCGAGTAGGGTCTTTGGCA+TGG	0.601597	3.2:-44256632	MS.gene050457:CDS
ATTGGATGCTCCAGACATTG+TGG	0.612151	3.2:-44257024	MS.gene050457:CDS
AAGATAAAACCATCAAGCCC+CGG	0.617625	3.2:-44257531	MS.gene050457:CDS
TGTTGTGGAACAAGAATGAG+AGG	0.618210	3.2:-44256233	MS.gene050457:CDS
GCTTGAACTCAGTTGACACT+GGG	0.627877	3.2:-44256272	MS.gene050457:CDS
CAGTAGCTAGTAAATTCCCT+TGG	0.629888	3.2:+44256349	None:intergenic
AATTTACTAGCTACTGGCGG+AGG	0.633586	3.2:-44256342	MS.gene050457:CDS
TCAAGCCAATGCCAATATGA+CGG	0.636859	3.2:+44256835	None:intergenic
GAGCCCAGATGGTTGGACAG+TGG	0.641101	3.2:-44256094	MS.gene050457:CDS
TCTGGTCTACAATTGGCCAG+TGG	0.642557	3.2:-44256480	MS.gene050457:CDS
ACCACATACTCACAACCGGA+GGG	0.650856	3.2:-44256605	MS.gene050457:CDS
GCACTACATGGTGACTGAAG+GGG	0.656801	3.2:-44257734	MS.gene050457:CDS
AGTTTGCGGATTGAAATGGT+CGG	0.668119	3.2:-44256505	MS.gene050457:CDS
GTTCAAGCATGCACCTGTGT+GGG	0.668846	3.2:+44256287	None:intergenic
CTGCAGCGGATGAAACCTTG+AGG	0.678181	3.2:-44256065	MS.gene050457:CDS
AGTTCAAGCATGCACCTGTG+TGG	0.678713	3.2:+44256286	None:intergenic
ACATTGTTGAAACATACAGA+GGG	0.688874	3.2:-44256539	MS.gene050457:CDS
AACCACATACTCACAACCGG+AGG	0.695066	3.2:-44256606	MS.gene050457:CDS
TGAGAGAAGAAATCAACTGG+CGG	0.700989	3.2:+44257583	None:intergenic
CACATTGTTGAAACATACAG+AGG	0.702329	3.2:-44256540	MS.gene050457:CDS
GGGATAGAAGTACTTCAACA+AGG	0.702662	3.2:-44256431	MS.gene050457:CDS
GGGAATTTACTAGCTACTGG+CGG	0.715639	3.2:-44256345	MS.gene050457:CDS
ATGTTACTACTTATCGAATG+CGG	0.717524	3.2:+44255962	None:intergenic
GTTGTGGAACAAGAATGAGA+GGG	0.727155	3.2:-44256232	MS.gene050457:CDS
ACCTGAGGAATAATTCGCCG+GGG	0.733838	3.2:+44257514	None:intergenic
ATAGAAGTACTTCAACAAGG+TGG	0.753913	3.2:-44256428	MS.gene050457:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATATTAGAAAACTAGTAGT+AGG	+	chr3.2:44257052-44257071	None:intergenic	20.0%
!!!	CACAAAATTGCAAAATATTT+TGG	+	chr3.2:44256668-44256687	None:intergenic	20.0%
!!!	GATTTAAGAATTTTGAACTT+TGG	-	chr3.2:44256496-44256515	MS.gene050457:CDS	20.0%
!!!	TGTTTTCTTAATCGTTTTTA+TGG	-	chr3.2:44256419-44256438	MS.gene050457:CDS	20.0%
!	ATGATAATCAACTTTACATC+TGG	-	chr3.2:44257301-44257320	MS.gene050457:intron	25.0%
!	TGATAATCAACTTTACATCT+GGG	-	chr3.2:44257302-44257321	MS.gene050457:intron	25.0%
!!	CACAAAATTGCAAAATCTTT+TGG	+	chr3.2:44256561-44256580	None:intergenic	25.0%
!!	GATTTGAGAATTTTGAACTT+CGG	-	chr3.2:44256603-44256622	MS.gene050457:CDS	25.0%
!!!	ATCAGTTTTTGAAAATCAAG+TGG	-	chr3.2:44256388-44256407	MS.gene050457:CDS	25.0%
!!!	TCAGTTTTTGAAAATCAAGT+GGG	-	chr3.2:44256389-44256408	MS.gene050457:CDS	25.0%
!!!	TTTTGTGCTAATTGATTTGT+TGG	-	chr3.2:44256574-44256593	MS.gene050457:CDS	25.0%
	AAATCCATAGCTGATCTATT+CGG	+	chr3.2:44255990-44256009	None:intergenic	30.0%
	AATGTTCTTGCAATTGGATT+AGG	-	chr3.2:44256790-44256809	MS.gene050457:intron	30.0%
	ACATTGTTGAAACATACAGA+GGG	-	chr3.2:44257214-44257233	MS.gene050457:intron	30.0%
	AGGAAACACATTGTATTTGT+GGG	-	chr3.2:44256810-44256829	MS.gene050457:CDS	30.0%
	ATAACGATGTATCGTTTGAA+GGG	+	chr3.2:44257009-44257028	None:intergenic	30.0%
	ATTACCTCAATTTACTGGAT+TGG	-	chr3.2:44256758-44256777	MS.gene050457:intron	30.0%
	TAATCCAATTGCAAGAACAT+TGG	+	chr3.2:44256791-44256810	None:intergenic	30.0%
	TAGGAAACACATTGTATTTG+TGG	-	chr3.2:44256809-44256828	MS.gene050457:CDS	30.0%
	TGACTATTACCTCAATTTAC+TGG	-	chr3.2:44256753-44256772	MS.gene050457:intron	30.0%
	TTACCTCAATTTACTGGATT+GGG	-	chr3.2:44256759-44256778	MS.gene050457:intron	30.0%
!	ACTTTGGAAATATCCTTCAA+TGG	-	chr3.2:44257584-44257603	MS.gene050457:CDS	30.0%
!!	AGACAGGTTTTATACTTATC+TGG	-	chr3.2:44256979-44256998	MS.gene050457:CDS	30.0%
!!	TCTTATCTGAAAGTGTTGAA+TGG	-	chr3.2:44256636-44256655	MS.gene050457:CDS	30.0%
!!	TCTTGTGCTAATTGATTTGT+TGG	-	chr3.2:44256467-44256486	MS.gene050457:CDS	30.0%
	AATAACCACATACTCACAAC+CGG	-	chr3.2:44257144-44257163	MS.gene050457:intron	35.0%
	ATATCCTTCAATGGTGAAGA+TGG	-	chr3.2:44257593-44257612	MS.gene050457:CDS	35.0%
	ATTTCAATCCGCAAACTTCT+CGG	+	chr3.2:44257245-44257264	None:intergenic	35.0%
	CACAGAATCAGATAACACTT+TGG	-	chr3.2:44257568-44257587	MS.gene050457:CDS	35.0%
	CACATTGTTGAAACATACAG+AGG	-	chr3.2:44257213-44257232	MS.gene050457:intron	35.0%
	CACTTCAAGAGTTCTTCATA+TGG	-	chr3.2:44257632-44257651	MS.gene050457:CDS	35.0%
	CATAACGATGTATCGTTTGA+AGG	+	chr3.2:44257010-44257029	None:intergenic	35.0%
	GTTCTTGAAAGCTAGAATTC+TGG	+	chr3.2:44256138-44256157	None:intergenic	35.0%
	TAATAGTCATCCACAATGTC+TGG	+	chr3.2:44256742-44256761	None:intergenic	35.0%
	TACCTCAATTTACTGGATTG+GGG	-	chr3.2:44256760-44256779	MS.gene050457:intron	35.0%
	TATTTGTGGGATGCATCAAA+CGG	-	chr3.2:44256823-44256842	MS.gene050457:CDS	35.0%
	TCATGAGAGAAGAAATCAAC+TGG	+	chr3.2:44256176-44256195	None:intergenic	35.0%
!	AGTTGAGAACTTTGAAAGGT+GGG	-	chr3.2:44257091-44257110	MS.gene050457:intron	35.0%
!	TGCATGAACTCTAGTTCAAA+CGG	-	chr3.2:44256335-44256354	MS.gene050457:CDS	35.0%
!!	AAAAGCATTCCAAAACCTCA+AGG	+	chr3.2:44257706-44257725	None:intergenic	35.0%
!!	AACAGTAACAAGTTCTGAAG+TGG	+	chr3.2:44256851-44256870	None:intergenic	35.0%
!!	ATAGAAGTACTTCAACAAGG+TGG	-	chr3.2:44257325-44257344	MS.gene050457:intron	35.0%
!!	GTTACTGTTGATGATGAAGA+TGG	-	chr3.2:44256862-44256881	MS.gene050457:CDS	35.0%
!!!	TTGAGGTTTTGGAATGCTTT+TGG	-	chr3.2:44257705-44257724	MS.gene050457:CDS	35.0%
	AAGATAAAACCATCAAGCCC+CGG	-	chr3.2:44256222-44256241	MS.gene050457:CDS	40.0%
	ATGCGGCTCAAATGTGAAAA+CGG	+	chr3.2:44257777-44257796	None:intergenic	40.0%
	CAAGGGAATTTACTAGCTAC+TGG	-	chr3.2:44257405-44257424	MS.gene050457:intron	40.0%
	CAGTAGCTAGTAAATTCCCT+TGG	+	chr3.2:44257407-44257426	None:intergenic	40.0%
	CATACCGAATAGATCAGCTA+TGG	-	chr3.2:44255983-44256002	MS.gene050457:CDS	40.0%
	CCCAACTTACAGATGTGATA+GGG	+	chr3.2:44256888-44256907	None:intergenic	40.0%
	CCCTATCACATCTGTAAGTT+GGG	-	chr3.2:44256885-44256904	MS.gene050457:CDS	40.0%
	CTAGTTCAAACGGCAAAAGT+CGG	-	chr3.2:44256345-44256364	MS.gene050457:CDS	40.0%
	CTCAACTGCAGAAACATAAC+AGG	+	chr3.2:44257078-44257097	None:intergenic	40.0%
	CTCATTCTTGTTCCACAACA+GGG	+	chr3.2:44257520-44257539	None:intergenic	40.0%
	CTGCAGTTGAGAACTTTGAA+AGG	-	chr3.2:44257087-44257106	MS.gene050457:intron	40.0%
	GCTAGTAAATTCCCTTGGAA+AGG	+	chr3.2:44257402-44257421	None:intergenic	40.0%
	GTGGGTATTCCAAAACTTGA+TGG	+	chr3.2:44257451-44257470	None:intergenic	40.0%
	GTTGTGGAACAAGAATGAGA+GGG	-	chr3.2:44257521-44257540	MS.gene050457:CDS	40.0%
	TCAAGCCAATGCCAATATGA+CGG	+	chr3.2:44256921-44256940	None:intergenic	40.0%
	TCTCATTCTTGTTCCACAAC+AGG	+	chr3.2:44257521-44257540	None:intergenic	40.0%
	TGAGAGAAGAAATCAACTGG+CGG	+	chr3.2:44256173-44256192	None:intergenic	40.0%
	TGTTGTGGAACAAGAATGAG+AGG	-	chr3.2:44257520-44257539	MS.gene050457:CDS	40.0%
	TTCCCCAATCCAGTAAATTG+AGG	+	chr3.2:44256765-44256784	None:intergenic	40.0%
	TTGATTATCATTCCCTCCAC+TGG	+	chr3.2:44257292-44257311	None:intergenic	40.0%
	TTTCGATTATGCGCACTACA+TGG	-	chr3.2:44256007-44256026	MS.gene050457:CDS	40.0%
!	AGTTTGCGGATTGAAATGGT+CGG	-	chr3.2:44257248-44257267	MS.gene050457:intron	40.0%
!	CAGTTGAGAACTTTGAAAGG+TGG	-	chr3.2:44257090-44257109	MS.gene050457:intron	40.0%
!	GAGAAGTTTGCGGATTGAAA+TGG	-	chr3.2:44257244-44257263	MS.gene050457:intron	40.0%
!	GCGATGAAACCATCAAGTTT+TGG	-	chr3.2:44257439-44257458	MS.gene050457:intron	40.0%
!!	CAGAAGCTCTAAACATGAAC+AGG	-	chr3.2:44256111-44256130	MS.gene050457:CDS	40.0%
!!	GGGATAGAAGTACTTCAACA+AGG	-	chr3.2:44257322-44257341	MS.gene050457:intron	40.0%
!!	TTGTTTTGCAGACTTCTGAG+AGG	-	chr3.2:44256704-44256723	MS.gene050457:intron	40.0%
!!!	AAGTTTTGGAATACCCACAC+AGG	-	chr3.2:44257453-44257472	MS.gene050457:intron	40.0%
	AATTCTACCATCCATCCCTC+CGG	+	chr3.2:44257166-44257185	None:intergenic	45.0%
	AATTTACTAGCTACTGGCGG+AGG	-	chr3.2:44257411-44257430	MS.gene050457:intron	45.0%
	ACACCTGAGGAATAATTCGC+CGG	+	chr3.2:44256244-44256263	None:intergenic	45.0%
	ACTGAAGGGGCTAAAGCTAA+AGG	-	chr3.2:44256032-44256051	MS.gene050457:CDS	45.0%
	AGCGCCAATGTTCTTGCAAT+TGG	-	chr3.2:44256784-44256803	MS.gene050457:intron	45.0%
	AGGGGCTAAAGCTAAAGGTA+AGG	-	chr3.2:44256037-44256056	MS.gene050457:CDS	45.0%
	ATTGGATGCTCCAGACATTG+TGG	-	chr3.2:44256729-44256748	MS.gene050457:intron	45.0%
	CTGCAGTAAAAGCTCTTGCT+TGG	-	chr3.2:44257373-44257392	MS.gene050457:intron	45.0%
	GCCCAACTTACAGATGTGAT+AGG	+	chr3.2:44256889-44256908	None:intergenic	45.0%
	GCCCTATCACATCTGTAAGT+TGG	-	chr3.2:44256884-44256903	MS.gene050457:CDS	45.0%
	GCTTGAACTCAGTTGACACT+GGG	-	chr3.2:44257481-44257500	MS.gene050457:intron	45.0%
	GGATACAGCTTCAGATAGAC+AGG	-	chr3.2:44256963-44256982	MS.gene050457:CDS	45.0%
	GGGAATTTACTAGCTACTGG+CGG	-	chr3.2:44257408-44257427	MS.gene050457:intron	45.0%
	GTGAAGATGGCAGAACTCAA+TGG	-	chr3.2:44257606-44257625	MS.gene050457:CDS	45.0%
	TAGCTTCCTGTAAGCTTCTC+TGG	+	chr3.2:44256087-44256106	None:intergenic	45.0%
	TCTGCCATCTTCACCATTGA+AGG	+	chr3.2:44257600-44257619	None:intergenic	45.0%
	TGCTTGAACTCAGTTGACAC+TGG	-	chr3.2:44257480-44257499	MS.gene050457:intron	45.0%
	TTCCTGTAAGCTTCTCTGGA+AGG	+	chr3.2:44256083-44256102	None:intergenic	45.0%
!	CGGATGAAACCTTGAGGTTT+TGG	-	chr3.2:44257694-44257713	MS.gene050457:CDS	45.0%
!	GAACTTTGAAAGGTGGGCAT+AGG	-	chr3.2:44257097-44257116	MS.gene050457:intron	45.0%
!	GCAGACTTCTGAGAGGATAT+TGG	-	chr3.2:44256711-44256730	MS.gene050457:intron	45.0%
!!	ACAACTCTGAAGTGCAGCTA+TGG	-	chr3.2:44256941-44256960	MS.gene050457:CDS	45.0%
!!	CAACTCTGAAGTGCAGCTAT+GGG	-	chr3.2:44256942-44256961	MS.gene050457:CDS	45.0%
!!	GCAGCTTTTGTTGCTACTTC+TGG	+	chr3.2:44257735-44257754	None:intergenic	45.0%
!!	TTGCTTGGTGTCCTTTCCAA+GGG	-	chr3.2:44257388-44257407	MS.gene050457:intron	45.0%
!!!	ATATTTTATCATTATTATTT+TGG	-	chr3.2:44256311-44256330	MS.gene050457:CDS	5.0%
	AACCACATACTCACAACCGG+AGG	-	chr3.2:44257147-44257166	MS.gene050457:intron	50.0%
	AAGAAATCAACTGGCGGCGT+AGG	+	chr3.2:44256167-44256186	None:intergenic	50.0%
	AATAATTCGCCGGGGCTTGA+TGG	+	chr3.2:44256234-44256253	None:intergenic	50.0%
	ACCACATACTCACAACCGGA+GGG	-	chr3.2:44257148-44257167	MS.gene050457:intron	50.0%
	ACCTGAGGAATAATTCGCCG+GGG	+	chr3.2:44256242-44256261	None:intergenic	50.0%
	AGTTCAAGCATGCACCTGTG+TGG	+	chr3.2:44257470-44257489	None:intergenic	50.0%
	ATGCCAATATGACGGCCATC+AGG	+	chr3.2:44256913-44256932	None:intergenic	50.0%
	CACCTGAGGAATAATTCGCC+GGG	+	chr3.2:44256243-44256262	None:intergenic	50.0%
	CGCACTACATGGTGACTGAA+GGG	-	chr3.2:44256018-44256037	MS.gene050457:CDS	50.0%
	CTCAGGTATGTTCCCTGTTG+TGG	-	chr3.2:44257505-44257524	MS.gene050457:intron	50.0%
	CTCCTTCCAGAGAAGCTTAC+AGG	-	chr3.2:44256078-44256097	MS.gene050457:CDS	50.0%
	CTTCAACAAGGTGGCTTCAC+AGG	-	chr3.2:44257334-44257353	MS.gene050457:intron	50.0%
	GATGGCCGTCATATTGGCAT+TGG	-	chr3.2:44256913-44256932	MS.gene050457:CDS	50.0%
	GCACTACATGGTGACTGAAG+GGG	-	chr3.2:44256019-44256038	MS.gene050457:CDS	50.0%
	GTCTACAATTGGCCAGTGGA+GGG	-	chr3.2:44257277-44257296	MS.gene050457:intron	50.0%
	GTTCAAGCATGCACCTGTGT+GGG	+	chr3.2:44257469-44257488	None:intergenic	50.0%
	TCTGGTCTACAATTGGCCAG+TGG	-	chr3.2:44257273-44257292	MS.gene050457:intron	50.0%
	TTGCCACTGTCCAACCATCT+GGG	+	chr3.2:44257665-44257684	None:intergenic	50.0%
!	AACGGCTCAGCTCTTGCTTT+TGG	+	chr3.2:44257759-44257778	None:intergenic	50.0%
!	GGATTGAAATGGTCGGCTTC+TGG	-	chr3.2:44257255-44257274	MS.gene050457:intron	50.0%
!	GTCGGCTTCTGGTCTACAAT+TGG	-	chr3.2:44257266-44257285	MS.gene050457:intron	50.0%
!	TCTGTAAGTTGGGCTCCTGA+TGG	-	chr3.2:44256895-44256914	MS.gene050457:CDS	50.0%
!	TGGAAGGAGAGCACACTTGT+GGG	+	chr3.2:44256067-44256086	None:intergenic	50.0%
!!	CTTGCTTGGTGTCCTTTCCA+AGG	-	chr3.2:44257387-44257406	MS.gene050457:intron	50.0%
	AAATCAACTGGCGGCGTAGG+TGG	+	chr3.2:44256164-44256183	None:intergenic	55.0%
	AGCGAGTAGGGTCTTTGGCA+TGG	-	chr3.2:44257121-44257140	MS.gene050457:intron	55.0%
	AGGGAGCTACTCAGCTCTCA+TGG	-	chr3.2:44257540-44257559	MS.gene050457:CDS	55.0%
	CACACACACACACACACCTG+AGG	+	chr3.2:44256257-44256276	None:intergenic	55.0%
	CATACTCACAACCGGAGGGA+TGG	-	chr3.2:44257152-44257171	MS.gene050457:intron	55.0%
	CTCAGTTGACACTGGGTCTC+AGG	-	chr3.2:44257488-44257507	MS.gene050457:intron	55.0%
	CTGCAGCGGATGAAACCTTG+AGG	-	chr3.2:44257688-44257707	MS.gene050457:CDS	55.0%
	GCGCACTACATGGTGACTGA+AGG	-	chr3.2:44256017-44256036	MS.gene050457:CDS	55.0%
	GCTCCTGATGGCCGTCATAT+TGG	-	chr3.2:44256907-44256926	MS.gene050457:CDS	55.0%
	GGGCATGACCGAGAAGTTTG+CGG	-	chr3.2:44257234-44257253	MS.gene050457:intron	55.0%
	GGTCTACAATTGGCCAGTGG+AGG	-	chr3.2:44257276-44257295	MS.gene050457:intron	55.0%
	GTTGCCACTGTCCAACCATC+TGG	+	chr3.2:44257666-44257685	None:intergenic	55.0%
	TAGGCAGCGAGTAGGGTCTT+TGG	-	chr3.2:44257116-44257135	MS.gene050457:intron	55.0%
	TCCCTCCGGTTGTGAGTATG+TGG	+	chr3.2:44257152-44257171	None:intergenic	55.0%
!	CTGGAAGGAGAGCACACTTG+TGG	+	chr3.2:44256068-44256087	None:intergenic	55.0%
	CATATGGCGCAGAGCCCAGA+TGG	-	chr3.2:44257648-44257667	MS.gene050457:CDS	60.0%
	CTCACAACCGGAGGGATGGA+TGG	-	chr3.2:44257156-44257175	MS.gene050457:intron	60.0%
	GACAGTGGCAACAGCTGCAG+CGG	-	chr3.2:44257674-44257693	MS.gene050457:CDS	60.0%
	GAGCCCAGATGGTTGGACAG+TGG	-	chr3.2:44257659-44257678	MS.gene050457:CDS	60.0%
	GCCCCGGCGAATTATTCCTC+AGG	-	chr3.2:44256238-44256257	MS.gene050457:CDS	60.0%
	GTGGGCATAGGCAGCGAGTA+GGG	-	chr3.2:44257109-44257128	MS.gene050457:intron	60.0%
	CTAGCTACTGGCGGAGGCAG+TGG	-	chr3.2:44257417-44257436	MS.gene050457:intron	65.0%
	GGTGGGCATAGGCAGCGAGT+AGG	-	chr3.2:44257108-44257127	MS.gene050457:intron	65.0%
	TGGCGCAGAGCCCAGATGGT+TGG	-	chr3.2:44257652-44257671	MS.gene050457:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	44255972	44257803	44255972	ID=MS.gene050457
chr3.2	mRNA	44255972	44257803	44255972	ID=MS.gene050457.t1;Parent=MS.gene050457
chr3.2	exon	44257516	44257803	44257516	ID=MS.gene050457.t1.exon1;Parent=MS.gene050457.t1
chr3.2	CDS	44257516	44257803	44257516	ID=cds.MS.gene050457.t1;Parent=MS.gene050457.t1
chr3.2	exon	44256791	44257060	44256791	ID=MS.gene050457.t1.exon2;Parent=MS.gene050457.t1
chr3.2	CDS	44256791	44257060	44256791	ID=cds.MS.gene050457.t1;Parent=MS.gene050457.t1
chr3.2	exon	44255972	44256682	44255972	ID=MS.gene050457.t1.exon3;Parent=MS.gene050457.t1
chr3.2	CDS	44255972	44256682	44255972	ID=cds.MS.gene050457.t1;Parent=MS.gene050457.t1

Gene Sequence

>MS.gene050457

TTGGATAGATTCATACCGAATAGATCAGCTATGGATTTCGATTATGCGCACTACATGGTGACTGAAGGGGCTAAAGCTAAAGGTAAGGAAAACCCACAAGTGTGCTCTCCTTCCAGAGAAGCTTACAGGAAGCTACTTGCAGAAGCTCTAAACATGAACAGGACCAGAATTCTAGCTTTCAAGAACAAGCCACCTACGCCGCCAGTTGATTTCTTCTCTCATGATATCATTACTTCATCAACTCTTCGACAAGATAAAACCATCAAGCCCCGGCGAATTATTCCTCAGGTGTGTGTGTGTGTGTGTGTGTTTCTTAATGATATCTGATTCATATTTCATATATTTTATCATTATTATTTTGGTTGCATGAACTCTAGTTCAAACGGCAAAAGTCGGATTTTAAAATTTTGCACTTGATCAGTTTTTGAAAATCAAGTGGGTCTGAATTGTTTTCTTAATCGTTTTTATGGTGTGTGAAATCACAATAGTTTGCAATCTTGTGCTAATTGATTTGTTGGTTATCTGATTTAAGAATTTTGAACTTTGGTTTTCTTAATTCTTATCTGAAAGTGTTGTATGTTGTAAACCAAAAGATTTTGCAATTTTGTGCTAATTGATTTGTTGGTTATCTGATTTGAGAATTTTGAACTTCGGTTTTCTTAATTCTTATCTGAAAGTGTTGAATGGTGTAAACCAAAATATTTTGCAATTTTGTGCTAATTGATTGATTTGTTGTTTTGCAGACTTCTGAGAGGATATTGGATGCTCCAGACATTGTGGATGACTATTACCTCAATTTACTGGATTGGGGAAGCGCCAATGTTCTTGCAATTGGATTAGGAAACACATTGTATTTGTGGGATGCATCAAACGGTTCCACTTCAGAACTTGTTACTGTTGATGATGAAGATGGCCCTATCACATCTGTAAGTTGGGCTCCTGATGGCCGTCATATTGGCATTGGCTTGAACAACTCTGAAGTGCAGCTATGGGATACAGCTTCAGATAGACAGGTTTTATACTTATCTGGTTTACCCTTCAAACGATACATCGTTATGAGCTATTGAGTATATAATTCCTACTACTAGTTTTCTAATATTATTCCTGTTATGTTTCTGCAGTTGAGAACTTTGAAAGGTGGGCATAGGCAGCGAGTAGGGTCTTTGGCATGGAATAACCACATACTCACAACCGGAGGGATGGATGGTAGAATTATCAATAATGATGTAAGAATTAGAGCACACATTGTTGAAACATACAGAGGGCATGACCGAGAAGTTTGCGGATTGAAATGGTCGGCTTCTGGTCTACAATTGGCCAGTGGAGGGAATGATAATCAACTTTACATCTGGGATAGAAGTACTTCAACAAGGTGGCTTCACAGGCTTGAAGATCATACATCTGCAGTAAAAGCTCTTGCTTGGTGTCCTTTCCAAGGGAATTTACTAGCTACTGGCGGAGGCAGTGGCGATGAAACCATCAAGTTTTGGAATACCCACACAGGTGCATGCTTGAACTCAGTTGACACTGGGTCTCAGGTATGTTCCCTGTTGTGGAACAAGAATGAGAGGGAGCTACTCAGCTCTCATGGTTTTACACAGAATCAGATAACACTTTGGAAATATCCTTCAATGGTGAAGATGGCAGAACTCAATGGTCACACTTCAAGAGTTCTTCATATGGCGCAGAGCCCAGATGGTTGGACAGTGGCAACAGCTGCAGCGGATGAAACCTTGAGGTTTTGGAATGCTTTTGGCACTCCAGAAGTAGCAACAAAAGCTGCACCAAAAGCAAGAGCTGAGCCGTTTTCACATTTGAGCCGCATTCGATAA

Protein sequence

>MS.gene050457.t1

LDRFIPNRSAMDFDYAHYMVTEGAKAKGKENPQVCSPSREAYRKLLAEALNMNRTRILAFKNKPPTPPVDFFSHDIITSSTLRQDKTIKPRRIIPQTSERILDAPDIVDDYYLNLLDWGSANVLAIGLGNTLYLWDASNGSTSELVTVDDEDGPITSVSWAPDGRHIGIGLNNSEVQLWDTASDRQLRTLKGGHRQRVGSLAWNNHILTTGGMDGRIINNDVRIRAHIVETYRGHDREVCGLKWSASGLQLASGGNDNQLYIWDRSTSTRWLHRLEDHTSAVKALAWCPFQGNLLATGGGSGDETIKFWNTHTGACLNSVDTGSQVCSLLWNKNERELLSSHGFTQNQITLWKYPSMVKMAELNGHTSRVLHMAQSPDGWTVATAAADETLRFWNAFGTPEVATKAAPKARAEPFSHLSRIR