AlfalfaGEDB | MS.gene30172

M. sativa cultivar XinJiangDaYe / MS.gene30172

KEGG: K03363
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00400.35 PF12894.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30172.t1	XP_003600092.1	94.1	459	21	2	1	453	1	459	1.80E-255	891.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30172.t1	Q9SZA4	76.2	453	99	4	7	453	8	457	1.2e-202	707.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30172.t1	G7IY01	94.1	459	21	2	1	453	1	459	1.3e-255	891.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene30172	MS.gene066500	PPI
MS.gene30172	MS.gene23852	PPI
MS.gene30172	MS.gene91851	PPI
MS.gene30172	MS.gene46611	PPI
MS.gene30172	MS.gene36842	PPI
MS.gene30172	MS.gene066464	PPI
MS.gene30172	MS.gene07706	PPI
MS.gene30172	MS.gene012203	PPI
MS.gene30172	MS.gene014419	PPI
MS.gene30172	MS.gene022109	PPI
MS.gene30172	MS.gene98540	PPI
MS.gene30172	MS.gene62819	PPI
MS.gene30172	MS.gene30176	PPI
MS.gene30172	MS.gene65775	PPI
MS.gene30172	MS.gene99554	PPI
MS.gene30172	MS.gene80163	PPI
MS.gene30172	MS.gene42253	PPI
MS.gene30172	MS.gene049022	PPI
MS.gene30172	MS.gene55883	PPI
MS.gene30172	MS.gene033401	PPI
MS.gene30172	MS.gene33377	PPI
MS.gene30172	MS.gene51335	PPI
MS.gene30172	MS.gene82020	PPI
MS.gene30172	MS.gene045937	PPI
MS.gene30172	MS.gene068273	PPI
MS.gene30172	MS.gene06177	PPI
MS.gene30172	MS.gene51278	PPI
MS.gene30172	MS.gene69966	PPI
MS.gene30172	MS.gene011448	PPI
MS.gene30172	MS.gene052188	PPI
MS.gene30172	MS.gene87218	PPI
MS.gene30172	MS.gene85534	PPI
MS.gene064762	MS.gene30172	PPI
MS.gene30172	MS.gene060496	PPI
MS.gene30172	MS.gene014321	PPI
MS.gene30172	MS.gene064862	PPI
MS.gene30172	MS.gene23881	PPI
MS.gene30172	MS.gene37226	PPI
MS.gene30172	MS.gene066501	PPI
MS.gene30172	MS.gene062662	PPI
MS.gene30172	MS.gene027441	PPI
MS.gene30172	MS.gene21734	PPI
MS.gene30172	MS.gene30177	PPI
MS.gene30172	MS.gene84466	PPI
MS.gene30172	MS.gene70008	PPI
MS.gene30172	MS.gene008833	PPI
MS.gene30172	MS.gene67157	PPI
MS.gene30172	MS.gene23811	PPI
MS.gene30172	MS.gene071113	PPI
MS.gene30172	MS.gene25244	PPI
MS.gene30172	MS.gene69965	PPI
MS.gene30172	MS.gene82740	PPI
MS.gene30172	MS.gene48655	PPI
MS.gene30172	MS.gene64441	PPI
MS.gene30172	MS.gene066046	PPI
MS.gene30172	MS.gene027071	PPI
MS.gene027071	MS.gene30172	PPI
MS.gene30172	MS.gene23810	PPI
MS.gene30172	MS.gene027073	PPI
MS.gene30172	MS.gene027072	PPI
MS.gene30172	MS.gene65463	PPI
MS.gene30172	MS.gene29768	PPI
MS.gene063222	MS.gene30172	PPI
MS.gene30172	MS.gene039682	PPI
MS.gene30172	MS.gene067705	PPI
MS.gene30172	MS.gene71204	PPI
MS.gene30172	MS.gene025229	PPI
MS.gene30172	MS.gene41291	PPI
MS.gene30172	MS.gene049021	PPI
MS.gene30172	MS.gene043742	PPI
MS.gene010912	MS.gene30172	PPI
MS.gene30172	MS.gene027075	PPI
MS.gene30172	MS.gene022502	PPI
MS.gene30172	MS.gene050457	PPI
MS.gene30172	MS.gene66343	PPI
MS.gene30172	MS.gene99973	PPI
MS.gene30172	MS.gene69396	PPI
MS.gene30172	MS.gene30174	PPI
MS.gene30172	MS.gene26021	PPI
MS.gene81658	MS.gene30172	PPI
MS.gene30172	MS.gene064435	PPI
MS.gene84465	MS.gene30172	PPI
MS.gene30172	MS.gene91775	PPI
MS.gene30172	MS.gene25515	PPI
MS.gene30172	MS.gene056455	PPI
MS.gene30172	MS.gene89986	PPI
MS.gene30172	MS.gene78281	PPI
MS.gene30172	MS.gene81663	PPI
MS.gene30172	MS.gene050270	PPI
MS.gene30172	MS.gene34712	PPI
MS.gene30172	MS.gene058128	PPI
MS.gene30172	MS.gene20161	PPI
MS.gene30172	MS.gene81660	PPI
MS.gene30172	MS.gene52355	PPI
MS.gene30172	MS.gene037184	PPI
MS.gene30172	MS.gene004844	PPI
MS.gene30172	MS.gene068249	PPI
MS.gene30172	MS.gene99588	PPI
MS.gene30172	MS.gene82021	PPI
MS.gene30172	MS.gene24806	PPI
MS.gene30172	MS.gene038519	PPI
MS.gene30172	MS.gene27223	PPI
MS.gene30172	MS.gene045133	PPI
MS.gene30172	MS.gene042783	PPI
MS.gene30172	MS.gene20160	PPI
MS.gene30172	MS.gene26019	PPI
MS.gene30172	MS.gene97943	PPI
MS.gene30172	MS.gene067322	PPI
MS.gene30172	MS.gene050272	PPI
MS.gene30172	MS.gene63085	PPI
MS.gene30172	MS.gene69687	PPI
MS.gene30172	MS.gene050456	PPI
MS.gene064035	MS.gene30172	PPI
MS.gene30172	MS.gene51258	PPI
MS.gene30172	MS.gene32723	PPI
MS.gene30172	MS.gene067321	PPI
MS.gene30172	MS.gene010910	PPI
MS.gene30172	MS.gene29842	PPI
MS.gene30172	MS.gene053363	PPI
MS.gene30172	MS.gene90626	PPI
MS.gene30172	MS.gene066467	PPI
MS.gene30172	MS.gene024753	PPI
MS.gene30172	MS.gene34651	PPI
MS.gene30172	MS.gene75493	PPI
MS.gene30172	MS.gene32726	PPI
MS.gene30172	MS.gene71122	PPI
MS.gene30172	MS.gene55882	PPI
MS.gene30172	MS.gene33406	PPI
MS.gene66342	MS.gene30172	PPI
MS.gene30172	MS.gene35613	PPI
MS.gene30172	MS.gene24922	PPI
MS.gene30172	MS.gene060495	PPI
MS.gene30172	MS.gene052956	PPI
MS.gene30172	MS.gene037950	PPI
MS.gene030294	MS.gene30172	PPI
MS.gene30172	MS.gene027074	PPI
MS.gene30172	MS.gene51262	PPI
MS.gene30172	MS.gene75059	PPI
MS.gene30172	MS.gene24951	PPI
MS.gene30172	MS.gene069995	PPI
MS.gene30172	MS.gene26672	PPI
MS.gene30172	MS.gene06176	PPI
MS.gene30172	MS.gene92674	PPI
MS.gene30172	MS.gene014931	PPI
MS.gene30172	MS.gene77885	PPI
MS.gene30172	MS.gene21888	PPI
MS.gene30172	MS.gene97379	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30172.t1	MTR_3g051690	94.118	459	21	2	1	453	1	459	0.0	897
MS.gene30172.t1	MTR_5g008010	89.301	458	40	4	1	453	1	454	0.0	845
MS.gene30172.t1	MTR_7g106870	72.727	451	110	7	12	453	11	457	0.0	679
MS.gene30172.t1	MTR_7g451830	65.101	447	130	9	15	453	3	431	0.0	568
MS.gene30172.t1	MTR_7g047640	65.101	447	130	9	15	453	3	431	0.0	568
MS.gene30172.t1	MTR_7g057540	63.170	448	134	9	14	453	2	426	0.0	539
MS.gene30172.t1	MTR_7g055590	63.087	447	134	9	14	452	2	425	0.0	536
MS.gene30172.t1	MTR_7g053100	60.177	452	150	9	15	453	2	436	0.0	519
MS.gene30172.t1	MTR_7g034625	70.126	318	88	2	128	438	13	330	3.83e-165	468
MS.gene30172.t1	MTR_2g100540	49.886	439	165	12	25	453	4	397	8.13e-128	376
MS.gene30172.t1	MTR_2g100460	55.977	343	137	5	111	451	52	382	7.61e-125	368
MS.gene30172.t1	MTR_2g060710	44.282	411	207	8	33	429	26	428	1.50e-119	357
MS.gene30172.t1	MTR_3g067940	44.286	420	212	7	33	440	57	466	1.49e-115	347
MS.gene30172.t1	MTR_4g102510	42.959	419	207	7	33	432	51	456	7.29e-110	333
MS.gene30172.t1	MTR_4g415070	51.471	68	33	0	378	445	57	124	3.21e-16	79.7
MS.gene30172.t1	MTR_1g054300	26.736	288	174	13	155	427	134	399	2.31e-15	78.6
MS.gene30172.t1	MTR_1g054300	26.119	268	170	11	165	425	235	481	1.23e-14	76.3
MS.gene30172.t1	MTR_2g012630	26.941	219	149	8	158	373	35	245	7.61e-15	77.8
MS.gene30172.t1	MTR_2g012630	26.941	219	149	8	158	373	35	245	8.02e-15	77.4
MS.gene30172.t1	MTR_5g016370	23.693	287	163	8	147	425	312	550	1.02e-14	76.6
MS.gene30172.t1	MTR_6g004040	27.897	233	154	10	144	370	19	243	1.86e-14	76.3
MS.gene30172.t1	MTR_5g016350	22.997	287	179	7	141	426	230	475	5.10e-13	71.2
MS.gene30172.t1	MTR_5g016350	23.077	286	178	7	141	425	294	538	5.13e-13	71.6
MS.gene30172.t1	MTR_4g095058	24.382	283	168	11	147	426	293	532	3.61e-12	68.9
MS.gene30172.t1	MTR_4g095058	24.382	283	168	11	147	426	295	534	3.72e-12	68.9
MS.gene30172.t1	MTR_1g049000	40.426	94	46	3	204	288	8	100	8.59e-12	64.3
MS.gene30172.t1	MTR_3g106080	24.026	308	192	10	159	434	326	623	1.49e-11	67.0
MS.gene30172.t1	MTR_7g086360	23.415	205	150	4	224	426	53	252	2.12e-11	65.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30172.t1	AT4G33270	76.159	453	99	5	7	453	8	457	0.0	711
MS.gene30172.t1	AT4G33260	76.351	444	96	5	16	453	7	447	0.0	685
MS.gene30172.t1	AT4G33260	74.473	427	100	5	16	436	7	430	0.0	651
MS.gene30172.t1	AT5G27570	67.473	455	132	7	7	453	4	450	0.0	612
MS.gene30172.t1	AT5G27080	66.438	438	132	6	23	453	13	442	0.0	588
MS.gene30172.t1	AT5G26900	64.302	451	146	6	9	453	3	444	0.0	579
MS.gene30172.t1	AT5G27945	60.916	371	125	5	99	453	62	428	2.25e-155	447
MS.gene30172.t1	AT4G22910	42.512	414	213	7	33	432	62	464	1.66e-111	337
MS.gene30172.t1	AT4G11920	42.118	425	212	8	33	438	53	462	6.02e-110	333
MS.gene30172.t1	AT5G13840	51.475	305	145	3	131	435	164	465	6.92e-110	333
MS.gene30172.t1	AT5G13840	51.475	305	145	3	131	435	164	465	6.92e-110	333
MS.gene30172.t1	AT1G11160	28.155	206	131	7	150	344	16	215	6.07e-16	81.3
MS.gene30172.t1	AT1G11160	28.155	206	131	7	150	344	16	215	6.45e-16	80.9
MS.gene30172.t1	AT1G11160	28.155	206	131	7	150	344	16	215	6.80e-16	80.9
MS.gene30172.t1	AT5G08390	28.632	234	137	10	140	370	37	243	2.83e-15	79.0
MS.gene30172.t1	AT1G11160	30.247	162	105	6	185	344	18	173	7.88e-15	77.8
MS.gene30172.t1	AT1G61210	26.126	222	145	8	142	350	6	221	1.68e-14	76.6
MS.gene30172.t1	AT1G61210	26.126	222	145	8	142	350	6	221	1.83e-14	76.6
MS.gene30172.t1	AT1G61210	26.339	224	142	8	142	350	6	221	1.98e-14	76.6
MS.gene30172.t1	AT5G52820	28.226	248	154	12	186	427	244	473	2.58e-14	75.1
MS.gene30172.t1	AT5G23430	28.302	212	139	9	144	350	19	222	3.92e-13	72.0
MS.gene30172.t1	AT5G23430	28.302	212	139	9	144	350	19	222	3.92e-13	72.0
MS.gene30172.t1	AT5G23430	28.302	212	139	9	144	350	19	222	4.02e-13	72.0
MS.gene30172.t1	AT2G32700	23.361	244	171	8	186	425	555	786	7.96e-13	71.2
MS.gene30172.t1	AT2G32700	23.361	244	171	8	186	425	555	786	7.96e-13	71.2
MS.gene30172.t1	AT2G32700	23.361	244	171	8	186	425	555	786	7.96e-13	71.2
MS.gene30172.t1	AT2G32700	23.361	244	171	8	186	425	555	786	7.96e-13	71.2
MS.gene30172.t1	AT2G32700	23.361	244	171	8	186	425	555	786	7.96e-13	71.2
MS.gene30172.t1	AT2G32700	23.361	244	171	8	186	425	574	805	8.05e-13	71.2
MS.gene30172.t1	AT2G32700	23.361	244	171	8	186	425	553	784	8.24e-13	71.2
MS.gene30172.t1	AT5G25150	25.449	334	210	12	135	440	305	627	1.28e-12	70.5
MS.gene30172.t1	AT5G67320	25.000	284	165	12	147	426	330	569	1.41e-12	70.1
MS.gene30172.t1	AT5G25150	25.449	334	210	12	135	440	305	627	1.48e-12	70.1
MS.gene30172.t1	AT5G13480	31.319	182	115	7	162	340	241	415	1.06e-11	67.4
MS.gene30172.t1	AT5G13480	26.957	230	149	10	186	408	137	354	6.42e-11	65.1
MS.gene30172.t1	AT5G13480	31.319	182	115	7	162	340	218	392	1.22e-11	67.4
MS.gene30172.t1	AT5G13480	26.957	230	149	10	186	408	114	331	8.66e-11	64.7
MS.gene30172.t1	AT2G41500	25.203	246	134	8	186	429	342	539	1.29e-11	67.0
MS.gene30172.t1	AT2G41500	25.203	246	134	8	186	429	342	539	1.29e-11	67.0
MS.gene30172.t1	AT5G13480	31.319	182	115	7	162	340	218	392	1.35e-11	67.0
MS.gene30172.t1	AT5G13480	26.957	230	149	10	186	408	114	331	8.70e-11	64.7
MS.gene30172.t1	AT5G13480	31.319	182	115	7	162	340	230	404	1.70e-11	67.0
MS.gene30172.t1	AT1G04510	25.116	215	152	5	238	448	236	445	2.21e-11	66.2
MS.gene30172.t1	AT1G04510	25.116	215	152	5	238	448	236	445	2.29e-11	66.2
MS.gene30172.t1	AT2G33340	25.521	192	135	5	238	426	236	422	3.04e-11	65.9
MS.gene30172.t1	AT2G33340	25.521	192	135	5	238	426	236	422	3.04e-11	65.9
MS.gene30172.t1	AT2G33340	25.521	192	135	5	238	426	236	422	3.23e-11	65.5
MS.gene30172.t1	AT3G49660	22.800	250	179	8	182	425	71	312	5.06e-11	64.3

Find 129 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGATGAAACCTTGAGGTTT+TGG	0.193519	3.3:+43030258	MS.gene30172:CDS
GTGGGTATTCCAAAACTTAA+TGG	0.216864	3.3:-43030012	None:intergenic
TGACTATTACCTCAATTTAC+TGG	0.257532	3.3:+43029317	MS.gene30172:CDS
GTTGCCACTGTCCAACCATC+TGG	0.273358	3.3:-43030227	None:intergenic
TTACCTCAATTTACTGGATT+GGG	0.283267	3.3:+43029323	MS.gene30172:CDS
AATGTTCTTGCAATTGGATT+AGG	0.295419	3.3:+43029354	MS.gene30172:CDS
CTCCTTCCAGAGAAGCTTAC+AGG	0.301895	3.3:+43028753	MS.gene30172:CDS
GTGATGAAACCATTAAGTTT+TGG	0.317251	3.3:+43030003	MS.gene30172:CDS
ATGCGGCTCAAATGTGAAAA+TGG	0.324208	3.3:-43030338	None:intergenic
AGCGCCAATGTTCTTGCAAT+TGG	0.327966	3.3:+43029348	MS.gene30172:CDS
GTTCTTGAAAGCTAGAATTC+TGG	0.331154	3.3:-43028810	None:intergenic
TTCTCTCTAACAATGGCTTC+AGG	0.336781	3.3:+43028369	None:intergenic
TCAGACTTCTGAGAGGATAT+TGG	0.341248	3.3:+43029275	MS.gene30172:intron
GGATTGAAATGGTCGGCTTC+TGG	0.343844	3.3:+43029819	MS.gene30172:CDS
ATGATAATCAACTTTACATC+TGG	0.350065	3.3:+43029865	MS.gene30172:CDS
AATGGCTCAACTCTTGCTTT+TGG	0.357983	3.3:-43030320	None:intergenic
ATTACCTCAATTTACTGGAT+TGG	0.371678	3.3:+43029322	MS.gene30172:CDS
TAGCTTCCTGTAAGCTTCTC+TGG	0.375997	3.3:-43028759	None:intergenic
TCTCATTCTTGTTCCACAAC+AGG	0.376647	3.3:-43030082	None:intergenic
AAATCCATCGCTGATCTATT+CGG	0.383791	3.3:-43028662	None:intergenic
GCTCCTGATGGCCGTCATAT+TGG	0.389395	3.3:+43029471	MS.gene30172:CDS
ATTTCAATCCGCAAACTTCT+CGG	0.394694	3.3:-43029806	None:intergenic
AATAATTCGCCGGGGCTTGA+TGG	0.396021	3.3:-43028906	None:intergenic
CCCTATCACATCTGTAAGTT+GGG	0.396604	3.3:+43029449	MS.gene30172:CDS
TCTGCCATCTTCACCATTGA+AGG	0.397901	3.3:-43030161	None:intergenic
TAATAGTCATCCACGATGTC+TGG	0.406812	3.3:-43029303	None:intergenic
CTCAGTTGACACTGGGTCTC+AGG	0.408962	3.3:+43030052	MS.gene30172:CDS
CAGAAGCTTTAAACATGAAC+AGG	0.411753	3.3:+43028786	MS.gene30172:CDS
TAGGAAACACATTGTATTTG+TGG	0.412235	3.3:+43029373	MS.gene30172:CDS
CAAGGGAATTTACTAGCTAC+TGG	0.417964	3.3:+43029969	MS.gene30172:CDS
GCCCTATCACATCTGTAAGT+TGG	0.420766	3.3:+43029448	MS.gene30172:CDS
GAGAAGTTTGCGGATTGAAA+TGG	0.425267	3.3:+43029808	MS.gene30172:CDS
CTCAGGTATGTTCCCTGTTG+TGG	0.426699	3.3:+43030069	MS.gene30172:CDS
CTTGCTTGGTGTCCTTTCCA+AGG	0.428743	3.3:+43029951	MS.gene30172:CDS
TAGGCAGCGAGTAGGGTCTT+TGG	0.428851	3.3:+43029680	MS.gene30172:CDS
GTCGGCTTCTGGTCTACAAT+TGG	0.444050	3.3:+43029830	MS.gene30172:CDS
GCCCAACTTACAGATGTGAT+AGG	0.450468	3.3:-43029450	None:intergenic
AGGAAACACATTGTATTTGT+GGG	0.452538	3.3:+43029374	MS.gene30172:CDS
CTTCAACAAGGTGGCTTCAC+AGG	0.460645	3.3:+43029898	MS.gene30172:CDS
CTCCGTAAGTGTTCCTGAAG+AGG	0.466289	3.3:-43028432	None:intergenic
TTGCTTGGTGTCCTTTCCAA+GGG	0.471233	3.3:+43029952	MS.gene30172:CDS
CACAGAATCAGATAACACTT+TGG	0.480456	3.3:+43030132	MS.gene30172:CDS
TCATGAGAGAAGAAATCAAC+TGG	0.484334	3.3:-43028848	None:intergenic
ACAACTCTGAAGTGCAGCTA+TGG	0.484474	3.3:+43029505	MS.gene30172:CDS
GGATACAGCTTCAGATAGAC+AGG	0.484963	3.3:+43029527	MS.gene30172:CDS
ATGCCAATATGACGGCCATC+AGG	0.485428	3.3:-43029474	None:intergenic
TTCCTCTTCAGGAACACTTA+CGG	0.486142	3.3:+43028430	MS.gene30172:CDS
CAGTTCAAGAGTTCTTCATA+TGG	0.487010	3.3:+43030196	MS.gene30172:CDS
TTGATTATCATTCCCTCCAC+TGG	0.491219	3.3:-43029853	None:intergenic
TTGCCACTGTCCAACCATCT+GGG	0.492660	3.3:-43030226	None:intergenic
TGGAAGGAGAGCACACTTGT+GGG	0.493184	3.3:-43028739	None:intergenic
CTAGCTACTGGCGGAGGCAG+TGG	0.493388	3.3:+43029981	MS.gene30172:CDS
ACTTTGGAAATATCCTTCAA+TGG	0.495344	3.3:+43030148	MS.gene30172:CDS
GATGGCCGTCATATTGGCAT+TGG	0.495923	3.3:+43029477	MS.gene30172:CDS
ATATCCTTCAATGGTGAAGA+TGG	0.498891	3.3:+43030157	MS.gene30172:CDS
AGCTGAGAAATTTGAAAGGT+GGG	0.499223	3.3:+43029655	MS.gene30172:intron
CGCACTACATGGTGACTGAA+GGG	0.507766	3.3:+43028693	MS.gene30172:CDS
CTGGAAGGAGAGCACACTTG+TGG	0.508404	3.3:-43028740	None:intergenic
AAGAAATCAACTGGCGGCGT+AGG	0.510142	3.3:-43028839	None:intergenic
GGGCATGACCGAGAAGTTTG+CGG	0.518071	3.3:+43029798	MS.gene30172:CDS
AATAACCACATACTCACAAC+CGG	0.531617	3.3:+43029708	MS.gene30172:CDS
TCCCTCCGGTTGTGAGTATG+TGG	0.533027	3.3:-43029713	None:intergenic
GCCCCGGCGAATTATTCCTC+AGG	0.533857	3.3:+43028913	MS.gene30172:CDS
CTCATTCTTGTTCCACAACA+GGG	0.537252	3.3:-43030081	None:intergenic
CTGCAGTAAAAGCTCTTGCT+TGG	0.539760	3.3:+43029937	MS.gene30172:CDS
ACACCTGAGGAATAATTCGC+CGG	0.542147	3.3:-43028916	None:intergenic
TGATAATCAACTTTACATCT+GGG	0.543770	3.3:+43029866	MS.gene30172:CDS
TTTCGATTATGCGCACTACA+TGG	0.544215	3.3:+43028682	MS.gene30172:CDS
TGCTTGAACTCAGTTGACAC+TGG	0.544299	3.3:+43030044	MS.gene30172:CDS
GACAGTGGCAACAGCTGCAG+CGG	0.545471	3.3:+43030238	MS.gene30172:CDS
AGGGAGCTACTCAGCTCTCA+TGG	0.545560	3.3:+43030104	MS.gene30172:CDS
AACGGTAACAAGTTCTGAAG+TGG	0.549127	3.3:-43029412	None:intergenic
TATTTGTGGGATGCATCAAA+CGG	0.551368	3.3:+43029387	MS.gene30172:CDS
AAATCAACTGGCGGCGTAGG+TGG	0.551699	3.3:-43028836	None:intergenic
CAACTCTGAAGTGCAGCTAT+GGG	0.552386	3.3:+43029506	MS.gene30172:CDS
TAATCCAATTGCAAGAACAT+TGG	0.554610	3.3:-43029352	None:intergenic
CTCACAACCGGAGGGATGGA+TGG	0.555824	3.3:+43029720	MS.gene30172:CDS
CATATGGCGCAGAGCCCAGA+TGG	0.555918	3.3:+43030212	MS.gene30172:CDS
GTGGGCATAGGCAGCGAGTA+GGG	0.557831	3.3:+43029673	MS.gene30172:CDS
CACCTGAGGAATAATTCGCC+GGG	0.559324	3.3:-43028915	None:intergenic
GAAATTTGAAAGGTGGGCAT+AGG	0.561086	3.3:+43029661	MS.gene30172:CDS
TGGCGCAGAGCCCAGATGGT+TGG	0.562028	3.3:+43030216	MS.gene30172:CDS
TACCTCAATTTACTGGATTG+GGG	0.562441	3.3:+43029324	MS.gene30172:CDS
GCTAGTAAATTCCCTTGGAA+AGG	0.568517	3.3:-43029963	None:intergenic
CTGCAGCTGAGAAATTTGAA+AGG	0.569872	3.3:+43029651	MS.gene30172:intron
GTCTACAATTGGCCAGTGGA+GGG	0.570881	3.3:+43029841	MS.gene30172:CDS
GTGAAGATGGCAGAACTCAA+TGG	0.571074	3.3:+43030170	MS.gene30172:CDS
GCGCACTACATGGTGACTGA+AGG	0.571364	3.3:+43028692	MS.gene30172:CDS
CATACTCACAACCGGAGGGA+TGG	0.571933	3.3:+43029716	MS.gene30172:CDS
GGTCTACAATTGGCCAGTGG+AGG	0.575013	3.3:+43029840	MS.gene30172:CDS
TTCCCCAATCCAGTAAATTG+AGG	0.576674	3.3:-43029326	None:intergenic
TTCCTGTAAGCTTCTCTGGA+AGG	0.579286	3.3:-43028755	None:intergenic
ACTGAAGGGGCTAAAGCTAA+AGG	0.581037	3.3:+43028707	MS.gene30172:CDS
AGGGGCTAAAGCTAAAGGTA+AGG	0.581254	3.3:+43028712	MS.gene30172:CDS
TCTGTAAGTTGGGCTCCTGA+TGG	0.584539	3.3:+43029459	MS.gene30172:CDS
CCCAACTTACAGATGTGATA+GGG	0.585245	3.3:-43029449	None:intergenic
AATTCTACCATCCATCCCTC+CGG	0.585408	3.3:-43029727	None:intergenic
AAAAGCATTCCAAAACCTCA+AGG	0.587395	3.3:-43030267	None:intergenic
CAGCTGAGAAATTTGAAAGG+TGG	0.593578	3.3:+43029654	MS.gene30172:intron
GGTGGGCATAGGCAGCGAGT+AGG	0.596714	3.3:+43029672	MS.gene30172:CDS
AGCGAGTAGGGTCTTTGGCA+TGG	0.601597	3.3:+43029685	MS.gene30172:CDS
GTTACCGTTGATGATGAAGA+TGG	0.607070	3.3:+43029426	MS.gene30172:CDS
AAGATAAAACCATCAAGCCC+CGG	0.617625	3.3:+43028897	MS.gene30172:CDS
TGTTGTGGAACAAGAATGAG+AGG	0.618210	3.3:+43030084	MS.gene30172:CDS
AGGGCCATCTTCATCATCAA+CGG	0.626287	3.3:-43029430	None:intergenic
GCTTGAACTCAGTTGACACT+GGG	0.627877	3.3:+43030045	MS.gene30172:CDS
CAGTAGCTAGTAAATTCCCT+TGG	0.629888	3.3:-43029968	None:intergenic
AATTTACTAGCTACTGGCGG+AGG	0.633586	3.3:+43029975	MS.gene30172:CDS
TCAAGCCAATGCCAATATGA+CGG	0.636859	3.3:-43029482	None:intergenic
GAGCCCAGATGGTTGGACAG+TGG	0.641101	3.3:+43030223	MS.gene30172:CDS
TCTGGTCTACAATTGGCCAG+TGG	0.642557	3.3:+43029837	MS.gene30172:CDS
ATGTTACTGCTTATCGAATG+CGG	0.643437	3.3:-43030355	None:intergenic
ACCACATACTCACAACCGGA+GGG	0.650856	3.3:+43029712	MS.gene30172:CDS
CATACCGAATAGATCAGCGA+TGG	0.652740	3.3:+43028658	MS.gene30172:CDS
GCACTACATGGTGACTGAAG+GGG	0.656801	3.3:+43028694	MS.gene30172:CDS
AGTTTGCGGATTGAAATGGT+CGG	0.668119	3.3:+43029812	MS.gene30172:CDS
GTTCAAGCATGCACCTGTGT+GGG	0.668846	3.3:-43030030	None:intergenic
ATTGGATGCTCCAGACATCG+TGG	0.669103	3.3:+43029293	MS.gene30172:CDS
CTGCAGCGGATGAAACCTTG+AGG	0.678181	3.3:+43030252	MS.gene30172:CDS
AGTTCAAGCATGCACCTGTG+TGG	0.678713	3.3:-43030031	None:intergenic
ACATTGTTGAAACATACAGA+GGG	0.688874	3.3:+43029778	MS.gene30172:CDS
AACCACATACTCACAACCGG+AGG	0.695066	3.3:+43029711	MS.gene30172:CDS
TGAGAGAAGAAATCAACTGG+CGG	0.700989	3.3:-43028845	None:intergenic
CACATTGTTGAAACATACAG+AGG	0.702329	3.3:+43029777	MS.gene30172:CDS
GGGATAGAAGTACTTCAACA+AGG	0.702662	3.3:+43029886	MS.gene30172:CDS
GGGAATTTACTAGCTACTGG+CGG	0.715639	3.3:+43029972	MS.gene30172:CDS
GTTGTGGAACAAGAATGAGA+GGG	0.727155	3.3:+43030085	MS.gene30172:CDS
ACCTGAGGAATAATTCGCCG+GGG	0.733838	3.3:-43028914	None:intergenic
ATAGAAGTACTTCAACAAGG+TGG	0.753913	3.3:+43029889	MS.gene30172:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAGAAATTTTTTCATTATTA+GGG	+	chr3.3:43028476-43028495	MS.gene30172:intron	10.0%
!!!	TAAGAAATTTTTTCATTATT+AGG	+	chr3.3:43028475-43028494	MS.gene30172:intron	10.0%
!!!	ATATTTTATCATTATCGATT+TGG	+	chr3.3:43028981-43029000	MS.gene30172:intron	15.0%
!!!	CAAGTACAAAATTTTAAAAT+CGG	-	chr3.3:43029039-43029058	None:intergenic	15.0%
!!!	ATAATAATGTATCGATTGAA+GGG	-	chr3.3:43029573-43029592	None:intergenic	20.0%
!!!	ATTAGTTTTTGAAAATCAAG+TGG	+	chr3.3:43029059-43029078	MS.gene30172:intron	20.0%
!!!	CACAAAATAGCAAAATATTT+TGG	-	chr3.3:43029232-43029251	None:intergenic	20.0%
!!!	TTAGTTTTTGAAAATCAAGT+GGG	+	chr3.3:43029060-43029079	MS.gene30172:intron	20.0%
!	AATATTAGAAACCTAGTAGT+AGG	-	chr3.3:43029616-43029635	None:intergenic	25.0%
!	ATGATAATCAACTTTACATC+TGG	+	chr3.3:43029865-43029884	MS.gene30172:CDS	25.0%
!	TGATAATCAACTTTACATCT+GGG	+	chr3.3:43029866-43029885	MS.gene30172:CDS	25.0%
!!	GATTTGAGAATTTTGAACTT+CGG	+	chr3.3:43029167-43029186	MS.gene30172:intron	25.0%
!!	TGTTTTCGTAATTGTTTGTA+TGG	+	chr3.3:43029090-43029109	MS.gene30172:intron	25.0%
!!!	CATAATAATGTATCGATTGA+AGG	-	chr3.3:43029574-43029593	None:intergenic	25.0%
	AATGTTCTTGCAATTGGATT+AGG	+	chr3.3:43029354-43029373	MS.gene30172:CDS	30.0%
	ACATTGTTGAAACATACAGA+GGG	+	chr3.3:43029778-43029797	MS.gene30172:CDS	30.0%
	AGGAAACACATTGTATTTGT+GGG	+	chr3.3:43029374-43029393	MS.gene30172:CDS	30.0%
	ATTACCTCAATTTACTGGAT+TGG	+	chr3.3:43029322-43029341	MS.gene30172:CDS	30.0%
	CTATGTCATGATAACTAACT+AGG	+	chr3.3:43028548-43028567	MS.gene30172:intron	30.0%
	TAATCCAATTGCAAGAACAT+TGG	-	chr3.3:43029355-43029374	None:intergenic	30.0%
	TAGGAAACACATTGTATTTG+TGG	+	chr3.3:43029373-43029392	MS.gene30172:CDS	30.0%
	TGACTATTACCTCAATTTAC+TGG	+	chr3.3:43029317-43029336	MS.gene30172:CDS	30.0%
	TTACCTCAATTTACTGGATT+GGG	+	chr3.3:43029323-43029342	MS.gene30172:CDS	30.0%
!	ACTTTGGAAATATCCTTCAA+TGG	+	chr3.3:43030148-43030167	MS.gene30172:CDS	30.0%
!	GTGATGAAACCATTAAGTTT+TGG	+	chr3.3:43030003-43030022	MS.gene30172:CDS	30.0%
!!	AAAACGTGTTTGAGTTTTGA+TGG	-	chr3.3:43028412-43028431	None:intergenic	30.0%
!!	AGACAGGTTTTATACTTATC+TGG	+	chr3.3:43029543-43029562	MS.gene30172:intron	30.0%
!!	TCTTATCTGAAAGTGTTGTA+TGG	+	chr3.3:43029200-43029219	MS.gene30172:intron	30.0%
!!	TCTTGTGCTAATTGATTTGT+TGG	+	chr3.3:43029138-43029157	MS.gene30172:intron	30.0%
	AAATCCATCGCTGATCTATT+CGG	-	chr3.3:43028665-43028684	None:intergenic	35.0%
	AATAACCACATACTCACAAC+CGG	+	chr3.3:43029708-43029727	MS.gene30172:CDS	35.0%
	AGAAACCTAGTAGTAGGAAA+TGG	-	chr3.3:43029610-43029629	None:intergenic	35.0%
	AGCTGAGAAATTTGAAAGGT+GGG	+	chr3.3:43029655-43029674	MS.gene30172:intron	35.0%
	AGTATCCATTTCCTACTACT+AGG	+	chr3.3:43029602-43029621	MS.gene30172:intron	35.0%
	ATATCCTTCAATGGTGAAGA+TGG	+	chr3.3:43030157-43030176	MS.gene30172:CDS	35.0%
	ATTTCAATCCGCAAACTTCT+CGG	-	chr3.3:43029809-43029828	None:intergenic	35.0%
	CACAGAATCAGATAACACTT+TGG	+	chr3.3:43030132-43030151	MS.gene30172:CDS	35.0%
	CACATTGTTGAAACATACAG+AGG	+	chr3.3:43029777-43029796	MS.gene30172:CDS	35.0%
	CAGTTCAAGAGTTCTTCATA+TGG	+	chr3.3:43030196-43030215	MS.gene30172:CDS	35.0%
	GTGGGTATTCCAAAACTTAA+TGG	-	chr3.3:43030015-43030034	None:intergenic	35.0%
	GTTCTTGAAAGCTAGAATTC+TGG	-	chr3.3:43028813-43028832	None:intergenic	35.0%
	TACCTCAATTTACTGGATTG+GGG	+	chr3.3:43029324-43029343	MS.gene30172:CDS	35.0%
	TATTTGTGGGATGCATCAAA+CGG	+	chr3.3:43029387-43029406	MS.gene30172:CDS	35.0%
	TCATGAGAGAAGAAATCAAC+TGG	-	chr3.3:43028851-43028870	None:intergenic	35.0%
!	GCATGAGACTCTAGTTTAAA+CGG	+	chr3.3:43029006-43029025	MS.gene30172:intron	35.0%
!!	AAAAGCATTCCAAAACCTCA+AGG	-	chr3.3:43030270-43030289	None:intergenic	35.0%
!!	ATAGAAGTACTTCAACAAGG+TGG	+	chr3.3:43029889-43029908	MS.gene30172:CDS	35.0%
!!	ATGTTTTTCAGACTTCTGAG+AGG	+	chr3.3:43029268-43029287	MS.gene30172:intron	35.0%
!!	CAGAAGCTTTAAACATGAAC+AGG	+	chr3.3:43028786-43028805	MS.gene30172:CDS	35.0%
!!	TTGATTTGTTTGTGACCAGT+TGG	+	chr3.3:43028628-43028647	MS.gene30172:intron	35.0%
!!!	TTGAGGTTTTGGAATGCTTT+TGG	+	chr3.3:43030269-43030288	MS.gene30172:CDS	35.0%
	AAGATAAAACCATCAAGCCC+CGG	+	chr3.3:43028897-43028916	MS.gene30172:CDS	40.0%
	ATGCGGCTCAAATGTGAAAA+TGG	-	chr3.3:43030341-43030360	None:intergenic	40.0%
	CAAGGGAATTTACTAGCTAC+TGG	+	chr3.3:43029969-43029988	MS.gene30172:CDS	40.0%
	CAGCTGAGAAATTTGAAAGG+TGG	+	chr3.3:43029654-43029673	MS.gene30172:intron	40.0%
	CAGTAGCTAGTAAATTCCCT+TGG	-	chr3.3:43029971-43029990	None:intergenic	40.0%
	CCCAACTTACAGATGTGATA+GGG	-	chr3.3:43029452-43029471	None:intergenic	40.0%
	CCCTATCACATCTGTAAGTT+GGG	+	chr3.3:43029449-43029468	MS.gene30172:CDS	40.0%
	CTCATTCTTGTTCCACAACA+GGG	-	chr3.3:43030084-43030103	None:intergenic	40.0%
	CTGCAGCTGAGAAATTTGAA+AGG	+	chr3.3:43029651-43029670	MS.gene30172:intron	40.0%
	GAAATTTGAAAGGTGGGCAT+AGG	+	chr3.3:43029661-43029680	MS.gene30172:CDS	40.0%
	GCTAGTAAATTCCCTTGGAA+AGG	-	chr3.3:43029966-43029985	None:intergenic	40.0%
	GTTGTGGAACAAGAATGAGA+GGG	+	chr3.3:43030085-43030104	MS.gene30172:CDS	40.0%
	TAATAGTCATCCACGATGTC+TGG	-	chr3.3:43029306-43029325	None:intergenic	40.0%
	TCAAGCCAATGCCAATATGA+CGG	-	chr3.3:43029485-43029504	None:intergenic	40.0%
	TCGGTATGAATCTATCCAAC+TGG	-	chr3.3:43028646-43028665	None:intergenic	40.0%
	TCTCATTCTTGTTCCACAAC+AGG	-	chr3.3:43030085-43030104	None:intergenic	40.0%
	TGAGAGAAGAAATCAACTGG+CGG	-	chr3.3:43028848-43028867	None:intergenic	40.0%
	TGTTGTGGAACAAGAATGAG+AGG	+	chr3.3:43030084-43030103	MS.gene30172:CDS	40.0%
	TTCCCCAATCCAGTAAATTG+AGG	-	chr3.3:43029329-43029348	None:intergenic	40.0%
	TTCCTCTTCAGGAACACTTA+CGG	+	chr3.3:43028430-43028449	MS.gene30172:CDS	40.0%
	TTGATTATCATTCCCTCCAC+TGG	-	chr3.3:43029856-43029875	None:intergenic	40.0%
	TTTCGATTATGCGCACTACA+TGG	+	chr3.3:43028682-43028701	MS.gene30172:CDS	40.0%
!	AATGGCTCAACTCTTGCTTT+TGG	-	chr3.3:43030323-43030342	None:intergenic	40.0%
!	AGTTTGCGGATTGAAATGGT+CGG	+	chr3.3:43029812-43029831	MS.gene30172:CDS	40.0%
!	GAGAAGTTTGCGGATTGAAA+TGG	+	chr3.3:43029808-43029827	MS.gene30172:CDS	40.0%
!	TCAAACACGTTTTCCTCTTC+AGG	+	chr3.3:43028419-43028438	MS.gene30172:CDS	40.0%
!	TCAGACTTCTGAGAGGATAT+TGG	+	chr3.3:43029275-43029294	MS.gene30172:intron	40.0%
!!	AACGGTAACAAGTTCTGAAG+TGG	-	chr3.3:43029415-43029434	None:intergenic	40.0%
!!	ACGTGTTTGAGTTTTGATGG+CGG	-	chr3.3:43028409-43028428	None:intergenic	40.0%
!!	GGGATAGAAGTACTTCAACA+AGG	+	chr3.3:43029886-43029905	MS.gene30172:CDS	40.0%
!!	GTTACCGTTGATGATGAAGA+TGG	+	chr3.3:43029426-43029445	MS.gene30172:CDS	40.0%
!!!	AAGTTTTGGAATACCCACAC+AGG	+	chr3.3:43030017-43030036	MS.gene30172:CDS	40.0%
	AATTCTACCATCCATCCCTC+CGG	-	chr3.3:43029730-43029749	None:intergenic	45.0%
	AATTTACTAGCTACTGGCGG+AGG	+	chr3.3:43029975-43029994	MS.gene30172:CDS	45.0%
	ACACCTGAGGAATAATTCGC+CGG	-	chr3.3:43028919-43028938	None:intergenic	45.0%
	ACTGAAGGGGCTAAAGCTAA+AGG	+	chr3.3:43028707-43028726	MS.gene30172:CDS	45.0%
	AGCGCCAATGTTCTTGCAAT+TGG	+	chr3.3:43029348-43029367	MS.gene30172:CDS	45.0%
	AGGGCCATCTTCATCATCAA+CGG	-	chr3.3:43029433-43029452	None:intergenic	45.0%
	AGGGGCTAAAGCTAAAGGTA+AGG	+	chr3.3:43028712-43028731	MS.gene30172:CDS	45.0%
	CATACCGAATAGATCAGCGA+TGG	+	chr3.3:43028658-43028677	MS.gene30172:CDS	45.0%
	CTGCAGTAAAAGCTCTTGCT+TGG	+	chr3.3:43029937-43029956	MS.gene30172:CDS	45.0%
	GCCCAACTTACAGATGTGAT+AGG	-	chr3.3:43029453-43029472	None:intergenic	45.0%
	GCCCTATCACATCTGTAAGT+TGG	+	chr3.3:43029448-43029467	MS.gene30172:CDS	45.0%
	GCTTGAACTCAGTTGACACT+GGG	+	chr3.3:43030045-43030064	MS.gene30172:CDS	45.0%
	GGATACAGCTTCAGATAGAC+AGG	+	chr3.3:43029527-43029546	MS.gene30172:CDS	45.0%
	GGGAATTTACTAGCTACTGG+CGG	+	chr3.3:43029972-43029991	MS.gene30172:CDS	45.0%
	GTGAAGATGGCAGAACTCAA+TGG	+	chr3.3:43030170-43030189	MS.gene30172:CDS	45.0%
	TAGCTTCCTGTAAGCTTCTC+TGG	-	chr3.3:43028762-43028781	None:intergenic	45.0%
	TCTGCCATCTTCACCATTGA+AGG	-	chr3.3:43030164-43030183	None:intergenic	45.0%
	TGCTTGAACTCAGTTGACAC+TGG	+	chr3.3:43030044-43030063	MS.gene30172:CDS	45.0%
	TTCCTGTAAGCTTCTCTGGA+AGG	-	chr3.3:43028758-43028777	None:intergenic	45.0%
!	CGGATGAAACCTTGAGGTTT+TGG	+	chr3.3:43030258-43030277	MS.gene30172:CDS	45.0%
!!	ACAACTCTGAAGTGCAGCTA+TGG	+	chr3.3:43029505-43029524	MS.gene30172:CDS	45.0%
!!	CAACTCTGAAGTGCAGCTAT+GGG	+	chr3.3:43029506-43029525	MS.gene30172:CDS	45.0%
!!	GCAGCTTTTGTTGCTACTTC+TGG	-	chr3.3:43030299-43030318	None:intergenic	45.0%
!!	TTGCTTGGTGTCCTTTCCAA+GGG	+	chr3.3:43029952-43029971	MS.gene30172:CDS	45.0%
	AACCACATACTCACAACCGG+AGG	+	chr3.3:43029711-43029730	MS.gene30172:CDS	50.0%
	AAGAAATCAACTGGCGGCGT+AGG	-	chr3.3:43028842-43028861	None:intergenic	50.0%
	AATAATTCGCCGGGGCTTGA+TGG	-	chr3.3:43028909-43028928	None:intergenic	50.0%
	ACCACATACTCACAACCGGA+GGG	+	chr3.3:43029712-43029731	MS.gene30172:CDS	50.0%
	ACCTGAGGAATAATTCGCCG+GGG	-	chr3.3:43028917-43028936	None:intergenic	50.0%
	AGTTCAAGCATGCACCTGTG+TGG	-	chr3.3:43030034-43030053	None:intergenic	50.0%
	ATGCCAATATGACGGCCATC+AGG	-	chr3.3:43029477-43029496	None:intergenic	50.0%
	ATTGGATGCTCCAGACATCG+TGG	+	chr3.3:43029293-43029312	MS.gene30172:CDS	50.0%
	CACCTGAGGAATAATTCGCC+GGG	-	chr3.3:43028918-43028937	None:intergenic	50.0%
	CGCACTACATGGTGACTGAA+GGG	+	chr3.3:43028693-43028712	MS.gene30172:CDS	50.0%
	CTCAGGTATGTTCCCTGTTG+TGG	+	chr3.3:43030069-43030088	MS.gene30172:CDS	50.0%
	CTCCGTAAGTGTTCCTGAAG+AGG	-	chr3.3:43028435-43028454	None:intergenic	50.0%
	CTCCTTCCAGAGAAGCTTAC+AGG	+	chr3.3:43028753-43028772	MS.gene30172:CDS	50.0%
	CTTCAACAAGGTGGCTTCAC+AGG	+	chr3.3:43029898-43029917	MS.gene30172:CDS	50.0%
	GATGGCCGTCATATTGGCAT+TGG	+	chr3.3:43029477-43029496	MS.gene30172:CDS	50.0%
	GCACTACATGGTGACTGAAG+GGG	+	chr3.3:43028694-43028713	MS.gene30172:CDS	50.0%
	GTCTACAATTGGCCAGTGGA+GGG	+	chr3.3:43029841-43029860	MS.gene30172:CDS	50.0%
	GTTCAAGCATGCACCTGTGT+GGG	-	chr3.3:43030033-43030052	None:intergenic	50.0%
	TACACACACACACACACCTG+AGG	-	chr3.3:43028932-43028951	None:intergenic	50.0%
	TCTGGTCTACAATTGGCCAG+TGG	+	chr3.3:43029837-43029856	MS.gene30172:CDS	50.0%
	TTGCCACTGTCCAACCATCT+GGG	-	chr3.3:43030229-43030248	None:intergenic	50.0%
!	GGATTGAAATGGTCGGCTTC+TGG	+	chr3.3:43029819-43029838	MS.gene30172:CDS	50.0%
!	GTCGGCTTCTGGTCTACAAT+TGG	+	chr3.3:43029830-43029849	MS.gene30172:CDS	50.0%
!	TCTGTAAGTTGGGCTCCTGA+TGG	+	chr3.3:43029459-43029478	MS.gene30172:CDS	50.0%
!	TGGAAGGAGAGCACACTTGT+GGG	-	chr3.3:43028742-43028761	None:intergenic	50.0%
!!	CTTGCTTGGTGTCCTTTCCA+AGG	+	chr3.3:43029951-43029970	MS.gene30172:CDS	50.0%
	AAATCAACTGGCGGCGTAGG+TGG	-	chr3.3:43028839-43028858	None:intergenic	55.0%
	AGCGAGTAGGGTCTTTGGCA+TGG	+	chr3.3:43029685-43029704	MS.gene30172:CDS	55.0%
	AGGGAGCTACTCAGCTCTCA+TGG	+	chr3.3:43030104-43030123	MS.gene30172:CDS	55.0%
	CATACTCACAACCGGAGGGA+TGG	+	chr3.3:43029716-43029735	MS.gene30172:CDS	55.0%
	CTCAGTTGACACTGGGTCTC+AGG	+	chr3.3:43030052-43030071	MS.gene30172:CDS	55.0%
	CTGCAGCGGATGAAACCTTG+AGG	+	chr3.3:43030252-43030271	MS.gene30172:CDS	55.0%
	GCGCACTACATGGTGACTGA+AGG	+	chr3.3:43028692-43028711	MS.gene30172:CDS	55.0%
	GCTCCTGATGGCCGTCATAT+TGG	+	chr3.3:43029471-43029490	MS.gene30172:CDS	55.0%
	GGGCATGACCGAGAAGTTTG+CGG	+	chr3.3:43029798-43029817	MS.gene30172:CDS	55.0%
	GGTCTACAATTGGCCAGTGG+AGG	+	chr3.3:43029840-43029859	MS.gene30172:CDS	55.0%
	GTTGCCACTGTCCAACCATC+TGG	-	chr3.3:43030230-43030249	None:intergenic	55.0%
	TAGGCAGCGAGTAGGGTCTT+TGG	+	chr3.3:43029680-43029699	MS.gene30172:CDS	55.0%
	TCCCTCCGGTTGTGAGTATG+TGG	-	chr3.3:43029716-43029735	None:intergenic	55.0%
!	CTGGAAGGAGAGCACACTTG+TGG	-	chr3.3:43028743-43028762	None:intergenic	55.0%
	CATATGGCGCAGAGCCCAGA+TGG	+	chr3.3:43030212-43030231	MS.gene30172:CDS	60.0%
	CTCACAACCGGAGGGATGGA+TGG	+	chr3.3:43029720-43029739	MS.gene30172:CDS	60.0%
	GACAGTGGCAACAGCTGCAG+CGG	+	chr3.3:43030238-43030257	MS.gene30172:CDS	60.0%
	GAGCCCAGATGGTTGGACAG+TGG	+	chr3.3:43030223-43030242	MS.gene30172:CDS	60.0%
	GCCCCGGCGAATTATTCCTC+AGG	+	chr3.3:43028913-43028932	MS.gene30172:CDS	60.0%
	GTGGGCATAGGCAGCGAGTA+GGG	+	chr3.3:43029673-43029692	MS.gene30172:CDS	60.0%
	CTAGCTACTGGCGGAGGCAG+TGG	+	chr3.3:43029981-43030000	MS.gene30172:CDS	65.0%
	GGTGGGCATAGGCAGCGAGT+AGG	+	chr3.3:43029672-43029691	MS.gene30172:CDS	65.0%
	TGGCGCAGAGCCCAGATGGT+TGG	+	chr3.3:43030216-43030235	MS.gene30172:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	43028381	43030367	43028381	ID=MS.gene30172
chr3.3	mRNA	43028381	43030367	43028381	ID=MS.gene30172.t1;Parent=MS.gene30172
chr3.3	exon	43028381	43028473	43028381	ID=MS.gene30172.t1.exon1;Parent=MS.gene30172.t1
chr3.3	CDS	43028381	43028473	43028381	ID=cds.MS.gene30172.t1;Parent=MS.gene30172.t1
chr3.3	exon	43028647	43028934	43028647	ID=MS.gene30172.t1.exon2;Parent=MS.gene30172.t1
chr3.3	CDS	43028647	43028934	43028647	ID=cds.MS.gene30172.t1;Parent=MS.gene30172.t1
chr3.3	exon	43029279	43029548	43029279	ID=MS.gene30172.t1.exon3;Parent=MS.gene30172.t1
chr3.3	CDS	43029279	43029548	43029279	ID=cds.MS.gene30172.t1;Parent=MS.gene30172.t1
chr3.3	exon	43029657	43030367	43029657	ID=MS.gene30172.t1.exon4;Parent=MS.gene30172.t1
chr3.3	CDS	43029657	43030367	43029657	ID=cds.MS.gene30172.t1;Parent=MS.gene30172.t1

Gene Sequence

>MS.gene30172

ATGGCTTCAGGTTCTTTGAGTTCTTCCGCCATCAAAACTCAAACACGTTTTCCTCTTCAGGAACACTTACGGAGAAAGACTTCAAATGAAAATGTAAGAAATTTTTTCATTATTAGGGTTTCAAACTTTCAATGTAATTCTGTTTTGAAAAAGAATGAATTTTGAAACTATGTCATGATAACTAACTAGGTACTTTATTGTGAGAATTTCTTTATAAAAATTAAGTAGTTTATTAATTATTTGATGTTTGATTTGTTTGTGACCAGTTGGATAGATTCATACCGAATAGATCAGCGATGGATTTCGATTATGCGCACTACATGGTGACTGAAGGGGCTAAAGCTAAAGGTAAGGAAAACCCACAAGTGTGCTCTCCTTCCAGAGAAGCTTACAGGAAGCTACTTGCAGAAGCTTTAAACATGAACAGGACCAGAATTCTAGCTTTCAAGAACAAGCCACCTACGCCGCCAGTTGATTTCTTCTCTCATGATATCATTACTTCATCAACTCTTCGACAAGATAAAACCATCAAGCCCCGGCGAATTATTCCTCAGGTGTGTGTGTGTGTGTATTTCTTAATGATATCTATTCATATTTCATATATTTTATCATTATCGATTTGGTTGCATGAGACTCTAGTTTAAACGGCAAAAGTCCGATTTTAAAATTTTGTACTTGATTAGTTTTTGAAAATCAAGTGGGTCTGAGTTGTTTTCGTAATTGTTTGTATGGTGTGTGTAATCACAATAGTTTGCAATCTTGTGCTAATTGATTTGTTGGTTATCTGATTTGAGAATTTTGAACTTCGGTTTTCTTAATTCTTATCTGAAAGTGTTGTATGGTGTAAACCAAAATATTTTGCTATTTTGTGCTAATTGATTGATATGATGTTTTTCAGACTTCTGAGAGGATATTGGATGCTCCAGACATCGTGGATGACTATTACCTCAATTTACTGGATTGGGGAAGCGCCAATGTTCTTGCAATTGGATTAGGAAACACATTGTATTTGTGGGATGCATCAAACGGTTCCACTTCAGAACTTGTTACCGTTGATGATGAAGATGGCCCTATCACATCTGTAAGTTGGGCTCCTGATGGCCGTCATATTGGCATTGGCTTGAACAACTCTGAAGTGCAGCTATGGGATACAGCTTCAGATAGACAGGTTTTATACTTATCTGGTTTACCCTTCAATCGATACATTATTATGAGCTATTCAGTATCCATTTCCTACTACTAGGTTTCTAATATTATTCTTGTTATATTTCTGCAGCTGAGAAATTTGAAAGGTGGGCATAGGCAGCGAGTAGGGTCTTTGGCATGGAATAACCACATACTCACAACCGGAGGGATGGATGGTAGAATTATCAATAATGATGTAAGAATTAGAGCACACATTGTTGAAACATACAGAGGGCATGACCGAGAAGTTTGCGGATTGAAATGGTCGGCTTCTGGTCTACAATTGGCCAGTGGAGGGAATGATAATCAACTTTACATCTGGGATAGAAGTACTTCAACAAGGTGGCTTCACAGGCTTGAAGATCATACATCTGCAGTAAAAGCTCTTGCTTGGTGTCCTTTCCAAGGGAATTTACTAGCTACTGGCGGAGGCAGTGGTGATGAAACCATTAAGTTTTGGAATACCCACACAGGTGCATGCTTGAACTCAGTTGACACTGGGTCTCAGGTATGTTCCCTGTTGTGGAACAAGAATGAGAGGGAGCTACTCAGCTCTCATGGTTTTACACAGAATCAGATAACACTTTGGAAATATCCTTCAATGGTGAAGATGGCAGAACTCAATGGTCACAGTTCAAGAGTTCTTCATATGGCGCAGAGCCCAGATGGTTGGACAGTGGCAACAGCTGCAGCGGATGAAACCTTGAGGTTTTGGAATGCTTTTGGCACTCCAGAAGTAGCAACAAAAGCTGCACCAAAAGCAAGAGTTGAGCCATTTTCACATTTGAGCCGCATTCGATAA

Protein sequence

>MS.gene30172.t1

MASGSLSSSAIKTQTRFPLQEHLRRKTSNENLDRFIPNRSAMDFDYAHYMVTEGAKAKGKENPQVCSPSREAYRKLLAEALNMNRTRILAFKNKPPTPPVDFFSHDIITSSTLRQDKTIKPRRIIPQTSERILDAPDIVDDYYLNLLDWGSANVLAIGLGNTLYLWDASNGSTSELVTVDDEDGPITSVSWAPDGRHIGIGLNNSEVQLWDTASDRQLRNLKGGHRQRVGSLAWNNHILTTGGMDGRIINNDVRIRAHIVETYRGHDREVCGLKWSASGLQLASGGNDNQLYIWDRSTSTRWLHRLEDHTSAVKALAWCPFQGNLLATGGGSGDETIKFWNTHTGACLNSVDTGSQVCSLLWNKNERELLSSHGFTQNQITLWKYPSMVKMAELNGHSSRVLHMAQSPDGWTVATAAADETLRFWNAFGTPEVATKAAPKARVEPFSHLSRIR