AlfalfaGEDB | MS.gene29768

M. sativa cultivar XinJiangDaYe / MS.gene29768

KEGG: K09584
GO: GO:0003674 GO:0003824 GO:0008150 GO:0009987 GO:0016853 GO:0019725 GO:0042592 GO:0045454 GO:0050789 GO:0050794 GO:0065007 GO:0065008
PFAM: PF00085.23

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29768.t1	XP_003616851.1	99.1	435	4	0	1	435	1	435	7.10E-249	869.4

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29768.t1	Q67UF5	79.3	420	80	2	22	435	23	441	6.4e-198	691.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29768.t1	G7KH66	99.1	435	4	0	1	435	1	435	5.1e-249	869.4

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene006547	MS.gene29768	PPI
MS.gene014696	MS.gene29768	PPI
MS.gene027071	MS.gene29768	PPI
MS.gene027074	MS.gene29768	PPI
MS.gene028674	MS.gene29768	PPI
MS.gene030294	MS.gene29768	PPI
MS.gene049022	MS.gene29768	PPI
MS.gene058128	MS.gene29768	PPI
MS.gene060495	MS.gene29768	PPI
MS.gene060496	MS.gene29768	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29768.t1	MTR_5g084930	99.080	435	4	0	1	435	1	435	0.0	884
MS.gene29768.t1	MTR_2g021750	84.962	133	20	0	1	133	1	133	5.17e-73	233
MS.gene29768.t1	MTR_2g021750	44.037	109	60	1	156	264	26	133	2.06e-23	100
MS.gene29768.t1	MTR_7g112040	33.824	272	154	8	1	267	3	253	3.36e-40	147
MS.gene29768.t1	MTR_7g112040	42.308	130	67	4	157	280	23	150	2.80e-21	95.1
MS.gene29768.t1	MTR_1g087520	35.000	240	136	5	33	267	34	258	1.11e-39	146
MS.gene29768.t1	MTR_1g087520	40.541	148	70	4	28	172	147	279	1.83e-24	104
MS.gene29768.t1	MTR_1g087520	40.625	128	68	4	163	284	34	159	2.81e-19	89.4
MS.gene29768.t1	MTR_1g096790	40.351	114	59	4	30	136	28	139	7.13e-17	82.8
MS.gene29768.t1	MTR_1g096790	29.075	227	129	7	163	382	31	232	3.98e-15	77.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29768.t1	AT1G04980	72.748	444	111	4	1	435	5	447	0.0	680
MS.gene29768.t1	AT2G32920	73.709	426	107	3	14	435	16	440	0.0	656
MS.gene29768.t1	AT2G47470	36.250	240	133	5	33	267	28	252	4.17e-44	155
MS.gene29768.t1	AT2G47470	54.369	103	42	3	30	128	143	244	1.38e-27	110
MS.gene29768.t1	AT2G47470	39.370	127	71	3	163	284	28	153	1.13e-19	89.0
MS.gene29768.t1	AT2G47470	36.250	240	133	5	33	267	28	252	9.12e-44	156
MS.gene29768.t1	AT2G47470	54.369	103	42	3	30	128	143	244	1.34e-27	112
MS.gene29768.t1	AT2G47470	39.370	127	71	3	163	284	28	153	2.73e-19	89.0
MS.gene29768.t1	AT2G47470	36.250	240	133	5	33	267	28	252	9.89e-44	156
MS.gene29768.t1	AT2G47470	54.369	103	42	3	30	128	143	244	1.57e-27	112

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Find 108 sgRNAs with CRISPR-Local

Find 277 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGATGGAGAAATAATTG+AGG	0.562273	5.3:-68663522	MS.gene29768:CDS
AAACCACCAGTTGATTACCA+AGG	0.716041	5.3:-68665761	MS.gene29768:CDS
AAAGTTGGTGTTGGTGGGTA+TGG	0.390826	5.3:-68663808	MS.gene29768:CDS
AACACCTTTCTTAACGTTCA+AGG	0.373568	5.3:+68663768	None:intergenic
AACCGTAGAGAGCGTGAGAA+AGG	0.451953	5.3:+68668295	None:intergenic
AAGCTGGACGTGGAGGCAAA+GGG	0.583780	5.3:-68663601	MS.gene29768:CDS
AAGGCAGCTACTGTATTAAA+GGG	0.517489	5.3:-68666772	MS.gene29768:CDS
AATTCTTTGCTCCATGGTGC+GGG	0.458259	5.3:-68666825	MS.gene29768:CDS
ACCTTGGACTTGAAGTTGTT+AGG	0.419712	5.3:+68668246	None:intergenic
ACGTTCAAGGCTACTAATGC+TGG	0.481617	5.3:+68663781	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATAGATTCTACTTGAAGC+TGG	+	chr5.3:68664392-68664411	None:intergenic	30.0%
AACTCAAATTAAGGTCTGTT+TGG	-	chr5.3:68664537-68664556	MS.gene29768:CDS	30.0%
AATAAGGGGAAAACAACTTA+TGG	+	chr5.3:68664645-68664664	None:intergenic	30.0%
AATATTATGCCATAAAGCAC+CGG	-	chr5.3:68663968-68663987	MS.gene29768:intron	30.0%
AATTGTAACACTACACATGT+TGG	+	chr5.3:68666180-68666199	None:intergenic	30.0%
ACATGTGTAGTGTTACAATT+TGG	-	chr5.3:68666180-68666199	MS.gene29768:intron	30.0%
AGGAATGAAGAAATGAACAA+TGG	+	chr5.3:68665119-68665138	None:intergenic	30.0%
ATGCAGATAGCAAAAAATAG+CGG	+	chr5.3:68667020-68667039	None:intergenic	30.0%
ATTAACTCATGAGTCATAGA+GGG	+	chr5.3:68667623-68667642	None:intergenic	30.0%
CCTGTTATGATTTACATGTT+CGG	-	chr5.3:68665886-68665905	MS.gene29768:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	68663460	68668370	68663460	ID=MS.gene29768
chr5.3	mRNA	68663460	68668370	68663460	ID=MS.gene29768.t1;Parent=MS.gene29768
chr5.3	exon	68668248	68668370	68668248	ID=MS.gene29768.t1.exon1;Parent=MS.gene29768.t1
chr5.3	CDS	68668248	68668370	68668248	ID=cds.MS.gene29768.t1;Parent=MS.gene29768.t1
chr5.3	exon	68666720	68666878	68666720	ID=MS.gene29768.t1.exon2;Parent=MS.gene29768.t1
chr5.3	CDS	68666720	68666878	68666720	ID=cds.MS.gene29768.t1;Parent=MS.gene29768.t1
chr5.3	exon	68665718	68665831	68665718	ID=MS.gene29768.t1.exon3;Parent=MS.gene29768.t1
chr5.3	CDS	68665718	68665831	68665718	ID=cds.MS.gene29768.t1;Parent=MS.gene29768.t1
chr5.3	exon	68665216	68665379	68665216	ID=MS.gene29768.t1.exon4;Parent=MS.gene29768.t1
chr5.3	CDS	68665216	68665379	68665216	ID=cds.MS.gene29768.t1;Parent=MS.gene29768.t1
chr5.3	exon	68665003	68665102	68665003	ID=MS.gene29768.t1.exon5;Parent=MS.gene29768.t1
chr5.3	CDS	68665003	68665102	68665003	ID=cds.MS.gene29768.t1;Parent=MS.gene29768.t1
chr5.3	exon	68664495	68664671	68664495	ID=MS.gene29768.t1.exon6;Parent=MS.gene29768.t1
chr5.3	CDS	68664495	68664671	68664495	ID=cds.MS.gene29768.t1;Parent=MS.gene29768.t1
chr5.3	exon	68664260	68664405	68664260	ID=MS.gene29768.t1.exon7;Parent=MS.gene29768.t1
chr5.3	CDS	68664260	68664405	68664260	ID=cds.MS.gene29768.t1;Parent=MS.gene29768.t1
chr5.3	exon	68663727	68663873	68663727	ID=MS.gene29768.t1.exon8;Parent=MS.gene29768.t1
chr5.3	CDS	68663727	68663873	68663727	ID=cds.MS.gene29768.t1;Parent=MS.gene29768.t1
chr5.3	exon	68663460	68663637	68663460	ID=MS.gene29768.t1.exon9;Parent=MS.gene29768.t1
chr5.3	CDS	68663460	68663637	68663460	ID=cds.MS.gene29768.t1;Parent=MS.gene29768.t1

Gene Sequence

>MS.gene29768

ATGGCGAAACTCTCATTCATTGCTGTAACTATCTTTCTCTTCAACAATCTCATCCTTTCTCACGCTCTCTACGGTTCTTCCTCACCCGTTCTTCAACTCACTCCTAACAACTTCAAGTCCAAGGTTTAATCTTTTTTCAATTTTTCATTGACCCAGTTCGTGATCCCACTTTTTTAGCTTGTTTTTTCAAATGGGTATTGCGTAGAATTGTTAAATTTTGTAGTTTTATGCTTGAAAACTATCTGGGTGTTTTGTGTTTGGAATTTTGCTATGTTGCTGTATTGTGGTGTTTCTGTTTTTGTTGATTAGTGTGTGAATATGGTTGATTTTATGATTCTGGGTTTTGTGGAATTACAATTTTTTGATTTGTGTGATATATATAATGTAACAAATCTGTTGATTCATACTATTATGCAAGTATGTGTTAATGTTGTGTTTTATCATCTGAAAAAAATTAATAGGTTTTAATTTATATAAACTCTTAAAAAGATCTTTTAATTCATGTGTTAATATTATGCCATAAAGCACCGGCACTCTTCGGATTAGGCCTGTCCGACACAGACACTGACACATTTGTTTACATGGAATTATGTCATTTTCTAAAATTATTATCGATTTCCACATGTCAGTGTCCATGTCGTATTAGGTGTCCGTGCTTTCATAGATGTTGTGTATGTTTCGTTCTGTGGTGAGAAAAATTGATTTTGAAAGAATTGATTATGTGAAATTGATTAGCACTAAAATTTAGTTGAAGGTAAAGTGATTTTATGTTTGGATAGGTTCATGTAAAAGTGAGGTGAACAATAAATTTGAGTGTAAAAAACAATACCAGAATTAGAAGCTACAATTTCCAGCTTCAAGTAGAATCTACTTTGGAGGCAAAATCAATTATATTCTAAAAAAAATTCTAGAATTAGAAGCTACAATGTCCAGCTTCAAGTAGAATCTATTTTGAAGGCAAAATCAATTCTACTCATGGTGATTCATTAAGGACGTTTCGATTTAATCATAACTACTTCTAAAGTCACACATATGATTGCATGTGATTCATTGCTGTAAATATTTTTATACTAACTAAACTCAAATTAAGGTCTGTTTGGATTGTTTTATATGAACTTATCTATTTACATTAGCACTTATAAGACTGTTTGAGAGAACTTACGAAAATTGCTCATGACGTGTCCATAAGTTGTTTTCCCCTTATTTTCATAGGCTCTTCAAGATAGCTTATGAAAACAGTCTATAACTTGTATGAAAACATTTTGATTTTATTTGATCTTTTGTTATAAAGATAAATTATACATATGCACTTAATTAAGTTGTTTATCCAAACAGTCTTTTATACTATTGTATTATGATTGGACTTTGATATGATGAAATACTAGCCCCTCCCCAAGAAAATACGAATAATTTTTATGAGTTAGCTTTTTCTTTGCTTTATCTAGTTTGTAATGCATCAGGTTTTTATGGGTGCCATTTTGAATAATGTCAGGTTCTGAATTCAAATGGAGTTGTTCTTGTTGAATTCTTTGCTCCATGGTGCGGGCACTGTAAGGCTCTGACTCCTATATGGGAGAAGGCAGCTACTGTATTAAAGGGTGTAGTTACTGTTGCAGCACTTGATGCTGATGCTCACCAATCTCTGGCTCAGGTTTTCCATTGTTCATTTCTTCATTCCTCAGATTCAGTATTTAAATTTAAATGACATATATTAAAGTAAGACTATGCTGCATGTGTTGTTTTCATCTTACATTTAACCTTTATGAATATGAATATGTGTTATTATATGTTTCACATTTCTGTACAATGTACACTGGAAATACATACCTTTGATGGACCACGGACGATGCAAAACATGGTATTGAACCTGTTTAGATTGGTTTATTCGAGTTAACTATTGACATAAGTACCTGTTAGACAGTTAGGGAAAGTTTATGAAAACTGTGTATGACATGTTCATAAGCTCTATGATAACTTATGAAAGCAGCTTATAGCTTATATGAAAAACAGGTTGAATTTGTTTTATCTTTTTTATAGAAATAGTTTCTACATAAACACTTATATGATAAGTGCTTAATTAAGTTGTTAATCCCAATAGGGTCAAGTGTCCTCCTGTAACTATTTTAAATATGTGGCATATGATTAATTTGACGTCATCATTTTGATCATTTTAGATATAAAATTGTTTGATGTTTTAGGGCATGCCCAAGATCCTACCATGCTCTACATATTGTATATCCTTATATGTAAAGTTTTTCCTTCGTTACATTCCTAGTCTTCCCATTCTATGTAAAGCAACCAAAGAATAAATTACATTAGTGAATAAGAATATGTTTGTTGAGATTATTTATTTTCCTGTCGCTTACATGACATTTGTTATTGTTTGCATGATGAAATATTTATTCATCTAATGCTTGTATCTTTCTCCTGTTATGATTTACATGTTCGGCTATTTTCTTCATTTATTTTATTATAACTGTAAATTGCAAAATTTGAAAAAATCATTTAGATTTTTGTGTCTTTTCTTAATTCCCTTCAGGAATATGGTATTAGAGGATTTCCAACTATAAAGGTGTTTGCACCTGGAAAACCACCAGTTGATTACCAAGGAGCCAGAGATGTTAAACCAATCGCAGAATTTGCACTTCAACAGGTTTGTTGGAATCTCTTCTTGGATGTTTTAACATTTCCCTCCCTAAAGTTTCAATCAAATATTTCCAACATGTGTAGTGTTACAATTTGGATTTTGGATAGAGTAGATAGATGTCTTCTTTTTGTGGAAAACAACAATTTCTTGTACTTTTATGAAAAATTACTACAAATAAAGGAGAATTTGATTAATAACTTGAAAGGGAAACTCTTCTTGCAACATCACTTGGTAGTGTACAAAATTTCAGCAGAGTGAGAATATCAATGTATTGGGATTAGGGTTGACTGTTCAGTGACTACTGCTTGTAATATTGTGTGATTATAGTTTATTTGCATGTACAGGTAAAGGCTCTTTTGAAGGAACGGTTAAATGGAAAAGCAACAGGTGGATCAAATGAAAAAAAAGAAACCACCGCTTCTTCTTCGGTAGAACTAAACTCTAGTAACTTCGATGAATTGGTTATCAAAAGCAAAGAACTTTGGATTGTAGAATTTTTCGCACCTTGGTGAGTACTTCCTATATTTCAATTATTTATTACCTGACTGAACATGTAATTCTACTTTCAGATTTGCAGTTTCTAAATCTAATAACAACTTTTTCTTTTTTTATTTCTCAAAGGTGTGGCCATTGTAAAAAATTAGCTCCTGAGTGGAAAAGAGCATCTAATAATTTGAAAGGGAAGGTTAAACTGGGTCATGTTGACTGTGATGCTGACAAGGTAAGCTTATGGAGTATATCTAATCTCATAGTAGTTGATAAAAATTAGAATCAGTTTCGTCAAATAGCGGCTATAGCGTAGCAGAATTTTAACAAACTGTTATTGTTCGGTGATATACAATTTAGTAGAAAGTGTTGTCAAATAGCCAGCTATAGAAGCACTATAGCACTGTAGTGGAACTTTAACAAACCGCTATTTTTTGCTATCTGCATGATGATCAAGATGATCTCAACCTTACTTTTCAATTTATTTATATTATTAGTGTTCAATATTAAGCTTTTTATTCTGCCTTTCAGTTTATATTATTCTGACAGGATATTTTTTTTGTCAGTCTCTAATGAGCAGGTTCAATGTTCAAGGATTCCCAACAATCTTGGTATTTGGAGCCGATAAAGATACTCCAATTCCTTACGAGGGTGCTAGAACTGCATCAGCCATCGAATCATTCGCACTAGAGCAGCTGGAAACAAATGTTGCTCCTCCAGAAGTGACAGAGCTATATAGTCCAGTGAGTGTTTGTTCTCCTGCAAACTTTACTTGATTGTACACTGATTTGAAAGAAAGAATATAAGATATAAACATGAGTGTTGTCTATAGGATGTTTTGGAAGAGAAATGTGGTTCTGCTGCTATCTGTTTTGTTTCCTTCCTTCCCGACATTTTGGATTCCAAAGCGGAGGGGAGAAACAGATACCTTCAGCAGTTATTAACAGTTGCAGAGAAGTTCAAAAGGAGTCCTTACAGGCAAGTAAAAAAACATTTTGCTTACATTTCATAGTTCATACTTTCACATCCCTCTATGACTCATGAGTTAATCTTTCAAATTCAACTTTTTTGGTTTATACTATTGATTTTGTAAATGCTTTACAAACATGTTTAAATCAAGATAAAAGGCAACATGCATTGTCAGTGTAAACTAGTTTTACACTGACGTTCAATGTGAATCGTGCATGTTGCAGTTATTTTTAAATTGCGGTTGCAAAGTGTACTTACATGGAAATATAATGGGTTCTCATTGGATATCAGTGTAAAACTATTTTGCACCAACAATGCATCTTCATCAAACTCTTAAACCAGTCTTTGTTGACGAATGCTTAATGGTTTTTTGTTTGAATTTGGGTCTTGAACAGCTATGTATGGGCAGCTGCCGGTAAGCAGCCAGACCTTGAGCAGAAAGTTGGTGTTGGTGGGTATGGTTATCCAGCATTAGTAGCCTTGAACGTTAAGAAAGGTGTTTATGCTCCTCTCAAGAGTGCTTTTGAACTCGACCAAATTATGTAAGTCTATTTTTCTAATTTTATGCTAAATAGTTGGCGCTTTACTGAAGCAAACAAGTACTTGAACTTTATTGATTTGTTTTCTTCAGAGAATTTGTGAAAGAAGCTGGACGTGGAGGCAAAGGGAACTTGCCCCTAGGTGACACTCCTCCCACCATTGTAAAGACAGAACCATGGGATGGAAAAGATGGAGAAATAATTGAGGAAGATGAATTTTCTCTTGAAGAACTCATGGGAGAAGATGCCTCAACCAAAGATGAACTATAA

Protein sequence

>MS.gene29768.t1

MAKLSFIAVTIFLFNNLILSHALYGSSSPVLQLTPNNFKSKVLNSNGVVLVEFFAPWCGHCKALTPIWEKAATVLKGVVTVAALDADAHQSLAQEYGIRGFPTIKVFAPGKPPVDYQGARDVKPIAEFALQQVKALLKERLNGKATGGSNEKKETTASSSVELNSSNFDELVIKSKELWIVEFFAPWCGHCKKLAPEWKRASNNLKGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPIPYEGARTASAIESFALEQLETNVAPPEVTELYSPDVLEEKCGSAAICFVSFLPDILDSKAEGRNRYLQQLLTVAEKFKRSPYSYVWAAAGKQPDLEQKVGVGGYGYPALVALNVKKGVYAPLKSAFELDQIIEFVKEAGRGGKGNLPLGDTPPTIVKTEPWDGKDGEIIEEDEFSLEELMGEDASTKDEL