AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene027072

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027072.t1 MTR_3g051690 94.626 428 17 2 1 422 32 459 0.0 843
MS.gene027072.t1 MTR_5g008010 90.398 427 32 4 1 422 32 454 0.0 797
MS.gene027072.t1 MTR_7g106870 74.825 429 97 6 1 422 33 457 0.0 668
MS.gene027072.t1 MTR_7g451830 67.366 429 116 8 2 422 19 431 0.0 566
MS.gene027072.t1 MTR_7g047640 67.366 429 116 8 2 422 19 431 0.0 566
MS.gene027072.t1 MTR_7g057540 64.884 430 125 8 1 422 15 426 0.0 534
MS.gene027072.t1 MTR_7g055590 64.802 429 125 8 1 421 15 425 0.0 531
MS.gene027072.t1 MTR_7g053100 62.005 429 139 7 1 422 25 436 0.0 518
MS.gene027072.t1 MTR_7g034625 70.755 318 86 2 97 407 13 330 1.81e-166 471
MS.gene027072.t1 MTR_2g100540 50.580 431 158 12 2 422 12 397 1.48e-127 374
MS.gene027072.t1 MTR_2g100460 56.560 343 135 5 80 420 52 382 1.63e-126 371
MS.gene027072.t1 MTR_2g060710 44.282 411 207 8 2 398 26 428 2.48e-119 355
MS.gene027072.t1 MTR_3g067940 44.286 420 212 7 2 409 57 466 3.97e-116 348
MS.gene027072.t1 MTR_4g102510 43.198 419 206 7 2 401 51 456 1.04e-110 334
MS.gene027072.t1 MTR_4g415070 58.621 58 24 0 347 404 57 114 1.90e-16 80.1
MS.gene027072.t1 MTR_2g012630 27.397 219 148 8 127 342 35 245 2.76e-15 78.6
MS.gene027072.t1 MTR_1g054300 26.263 297 161 10 147 396 114 399 2.99e-15 77.8
MS.gene027072.t1 MTR_1g054300 26.119 268 170 11 134 394 235 481 2.20e-14 75.1
MS.gene027072.t1 MTR_2g012630 27.397 219 148 8 127 342 35 245 3.23e-15 78.6
MS.gene027072.t1 MTR_6g004040 28.326 233 153 10 113 339 19 243 6.90e-15 77.4
MS.gene027072.t1 MTR_5g016370 23.693 287 163 8 116 394 312 550 1.21e-14 76.3
MS.gene027072.t1 MTR_5g016350 22.997 287 179 7 110 395 294 539 5.78e-13 70.9
MS.gene027072.t1 MTR_5g016350 22.997 287 179 7 110 395 230 475 6.91e-13 70.5
MS.gene027072.t1 MTR_4g095058 24.382 283 168 11 116 395 295 534 5.37e-12 68.2
MS.gene027072.t1 MTR_4g095058 24.382 283 168 11 116 395 293 532 6.46e-12 67.8
MS.gene027072.t1 MTR_3g106080 24.351 308 191 10 128 403 326 623 9.18e-12 67.4
MS.gene027072.t1 MTR_7g086360 23.415 205 150 4 193 395 53 252 2.42e-11 65.1
MS.gene027072.t1 MTR_1g049000 39.362 94 47 3 173 257 8 100 4.80e-11 62.0
MS.gene027072.t1 MTR_4g119620 22.581 248 177 7 155 394 81 321 5.36e-11 63.9
MS.gene027072.t1 MTR_5g022690 27.011 174 120 5 139 310 266 434 8.50e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027072.t1 AT4G33270 77.283 427 89 4 1 422 34 457 0.0 687
MS.gene027072.t1 AT4G33260 77.283 427 89 4 1 422 24 447 0.0 668
MS.gene027072.t1 AT4G33260 75.122 410 94 4 1 405 24 430 0.0 635
MS.gene027072.t1 AT5G27570 68.531 429 120 6 1 422 30 450 0.0 590
MS.gene027072.t1 AT5G27080 67.133 429 126 6 1 422 22 442 0.0 580
MS.gene027072.t1 AT5G26900 66.589 428 129 5 1 422 25 444 0.0 572
MS.gene027072.t1 AT5G27945 61.456 371 123 5 68 422 62 428 1.21e-156 449
MS.gene027072.t1 AT4G22910 42.822 411 216 7 2 401 62 464 8.70e-113 339
MS.gene027072.t1 AT5G13840 51.475 305 145 3 100 404 164 465 1.56e-110 333
MS.gene027072.t1 AT5G13840 51.475 305 145 3 100 404 164 465 1.56e-110 333
MS.gene027072.t1 AT4G11920 42.118 425 212 8 2 407 53 462 1.91e-110 333
MS.gene027072.t1 AT1G11160 28.155 206 131 7 119 313 16 215 4.47e-16 81.3
MS.gene027072.t1 AT1G11160 28.155 206 131 7 119 313 16 215 4.67e-16 81.3
MS.gene027072.t1 AT1G11160 28.155 206 131 7 119 313 16 215 5.53e-16 80.9
MS.gene027072.t1 AT5G08390 29.060 234 136 10 109 339 37 243 1.16e-15 79.7
MS.gene027072.t1 AT1G11160 30.247 162 105 6 154 313 18 173 6.14e-15 77.8
MS.gene027072.t1 AT1G61210 26.339 224 142 8 111 319 6 221 1.68e-14 76.3
MS.gene027072.t1 AT1G61210 26.126 222 145 8 111 319 6 221 1.81e-14 76.3
MS.gene027072.t1 AT1G61210 26.339 224 142 8 111 319 6 221 1.95e-14 76.3
MS.gene027072.t1 AT5G52820 28.226 248 154 12 155 396 244 473 4.13e-14 74.3
MS.gene027072.t1 AT5G23430 28.774 212 138 9 113 319 19 222 1.64e-13 73.2
MS.gene027072.t1 AT5G23430 28.774 212 138 9 113 319 19 222 1.64e-13 73.2
MS.gene027072.t1 AT5G23430 28.774 212 138 9 113 319 19 222 1.79e-13 72.8
MS.gene027072.t1 AT5G25150 25.749 334 209 12 104 409 305 627 3.96e-13 71.6
MS.gene027072.t1 AT5G25150 25.749 334 209 12 104 409 305 627 4.70e-13 71.6
MS.gene027072.t1 AT2G32700 25.610 246 163 11 155 394 574 805 5.98e-13 71.2
MS.gene027072.t1 AT2G32700 25.610 246 163 11 155 394 553 784 6.06e-13 71.2
MS.gene027072.t1 AT2G32700 25.610 246 163 11 155 394 555 786 6.29e-13 71.2
MS.gene027072.t1 AT2G32700 25.610 246 163 11 155 394 555 786 6.29e-13 71.2
MS.gene027072.t1 AT2G32700 25.610 246 163 11 155 394 555 786 6.29e-13 71.2
MS.gene027072.t1 AT2G32700 25.610 246 163 11 155 394 555 786 6.29e-13 71.2
MS.gene027072.t1 AT2G32700 25.610 246 163 11 155 394 555 786 6.29e-13 71.2
MS.gene027072.t1 AT5G67320 25.000 284 165 12 116 395 330 569 1.94e-12 69.3
MS.gene027072.t1 AT3G49660 23.600 250 177 8 151 394 71 312 3.93e-12 67.4
MS.gene027072.t1 AT5G13480 31.319 182 115 7 131 309 218 392 1.02e-11 67.4
MS.gene027072.t1 AT5G13480 27.391 230 148 10 155 377 114 331 1.74e-11 66.6
MS.gene027072.t1 AT2G33340 26.042 192 134 5 207 395 236 422 1.11e-11 67.0
MS.gene027072.t1 AT2G33340 26.042 192 134 5 207 395 236 422 1.11e-11 67.0
MS.gene027072.t1 AT2G41500 25.203 246 134 8 155 398 342 539 1.16e-11 67.0
MS.gene027072.t1 AT2G41500 25.203 246 134 8 155 398 342 539 1.16e-11 67.0
MS.gene027072.t1 AT5G13480 31.319 182 115 7 131 309 241 415 1.17e-11 67.0
MS.gene027072.t1 AT5G13480 27.391 230 148 10 155 377 137 354 1.72e-11 66.6
MS.gene027072.t1 AT5G13480 31.319 182 115 7 131 309 218 392 1.22e-11 67.0
MS.gene027072.t1 AT5G13480 27.391 230 148 10 155 377 114 331 2.12e-11 66.2
MS.gene027072.t1 AT1G04510 25.116 215 152 5 207 417 236 445 1.34e-11 66.6
MS.gene027072.t1 AT2G33340 26.042 192 134 5 207 395 236 422 1.37e-11 66.6
MS.gene027072.t1 AT1G04510 25.116 215 152 5 207 417 236 445 1.55e-11 66.6
MS.gene027072.t1 AT5G13480 31.319 182 115 7 131 309 230 404 1.57e-11 66.6
MS.gene027072.t1 AT5G13480 27.391 230 148 10 155 377 126 343 2.41e-11 66.2
MS.gene027072.t1 AT4G02730 26.852 216 145 9 185 395 34 241 3.89e-11 64.3
MS.gene027072.t1 AT5G56130 23.786 206 113 5 232 395 16 219 7.10e-11 63.5

Find 125 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGATGAAACCTTGAGGTTT+TGG 0.193519 3.2:+44487034 MS.gene027072:CDS
TGACTATTACCTCAATTTAC+TGG 0.257532 3.2:+44486093 MS.gene027072:CDS
GTTGCCACTGTCCAACCATC+TGG 0.273358 3.2:-44487003 None:intergenic
AACGGCTCAGCTCTTGCTTT+TGG 0.277205 3.2:-44487096 None:intergenic
TTACCTCAATTTACTGGATT+GGG 0.283267 3.2:+44486099 MS.gene027072:CDS
AATGTTCTTGCAATTGGATT+AGG 0.295419 3.2:+44486130 MS.gene027072:CDS
CTCCTTCCAGAGAAGCTTAC+AGG 0.301895 3.2:+44485418 MS.gene027072:CDS
AGCGCCAATGTTCTTGCAAT+TGG 0.327966 3.2:+44486124 MS.gene027072:CDS
GTTCTTGAAAGCTAGAATTC+TGG 0.331154 3.2:-44485475 None:intergenic
GCAGACTTCTGAGAGGATAT+TGG 0.341248 3.2:+44486051 MS.gene027072:intron
GGATTGAAATGGTCGGCTTC+TGG 0.343844 3.2:+44486595 MS.gene027072:CDS
ATGATAATCAACTTTACATC+TGG 0.350065 3.2:+44486641 MS.gene027072:CDS
GCGATGAAACCATCAAGTTT+TGG 0.364930 3.2:+44486779 MS.gene027072:CDS
ATTACCTCAATTTACTGGAT+TGG 0.371678 3.2:+44486098 MS.gene027072:CDS
TAGCTTCCTGTAAGCTTCTC+TGG 0.375997 3.2:-44485424 None:intergenic
TCTCATTCTTGTTCCACAAC+AGG 0.376647 3.2:-44486858 None:intergenic
GCTCCTGATGGCCGTCATAT+TGG 0.389395 3.2:+44486247 MS.gene027072:CDS
ATTTCAATCCGCAAACTTCT+CGG 0.394694 3.2:-44486582 None:intergenic
AATAATTCGCCGGGGCTTGA+TGG 0.396021 3.2:-44485571 None:intergenic
CCCTATCACATCTGTAAGTT+GGG 0.396604 3.2:+44486225 MS.gene027072:CDS
TCTGCCATCTTCACCATTGA+AGG 0.397901 3.2:-44486937 None:intergenic
CTCAGTTGACACTGGGTCTC+AGG 0.408962 3.2:+44486828 MS.gene027072:CDS
TAGGAAACACATTGTATTTG+TGG 0.412235 3.2:+44486149 MS.gene027072:CDS
CAAGGGAATTTACTAGCTAC+TGG 0.417964 3.2:+44486745 MS.gene027072:CDS
GCCCTATCACATCTGTAAGT+TGG 0.420766 3.2:+44486224 MS.gene027072:CDS
GAGAAGTTTGCGGATTGAAA+TGG 0.425267 3.2:+44486584 MS.gene027072:CDS
CTCAGGTATGTTCCCTGTTG+TGG 0.426699 3.2:+44486845 MS.gene027072:CDS
TAATAGTCATCCACAATGTC+TGG 0.428065 3.2:-44486079 None:intergenic
CTTGCTTGGTGTCCTTTCCA+AGG 0.428743 3.2:+44486727 MS.gene027072:CDS
GTGGGTATTCCAAAACTTGA+TGG 0.428833 3.2:-44486788 None:intergenic
TAGGCAGCGAGTAGGGTCTT+TGG 0.428851 3.2:+44486456 MS.gene027072:CDS
CAGAAGCTCTAAACATGAAC+AGG 0.436496 3.2:+44485451 MS.gene027072:CDS
GTCGGCTTCTGGTCTACAAT+TGG 0.444050 3.2:+44486606 MS.gene027072:CDS
AAATCCATAGCTGATCTATT+CGG 0.447023 3.2:-44485327 None:intergenic
GCCCAACTTACAGATGTGAT+AGG 0.450468 3.2:-44486226 None:intergenic
AGGAAACACATTGTATTTGT+GGG 0.452538 3.2:+44486150 MS.gene027072:CDS
CTTCAACAAGGTGGCTTCAC+AGG 0.460645 3.2:+44486674 MS.gene027072:CDS
TTGCTTGGTGTCCTTTCCAA+GGG 0.471233 3.2:+44486728 MS.gene027072:CDS
CACAGAATCAGATAACACTT+TGG 0.480456 3.2:+44486908 MS.gene027072:CDS
TCATGAGAGAAGAAATCAAC+TGG 0.484334 3.2:-44485513 None:intergenic
ACAACTCTGAAGTGCAGCTA+TGG 0.484474 3.2:+44486281 MS.gene027072:CDS
GGATACAGCTTCAGATAGAC+AGG 0.484963 3.2:+44486303 MS.gene027072:CDS
ATGCCAATATGACGGCCATC+AGG 0.485428 3.2:-44486250 None:intergenic
CTAGCTACTGGCGGAGGCAG+TGG 0.487153 3.2:+44486757 MS.gene027072:CDS
CACTTCAAGAGTTCTTCATA+TGG 0.487383 3.2:+44486972 MS.gene027072:CDS
TTGATTATCATTCCCTCCAC+TGG 0.491219 3.2:-44486629 None:intergenic
TTGCCACTGTCCAACCATCT+GGG 0.492660 3.2:-44487002 None:intergenic
TGGAAGGAGAGCACACTTGT+GGG 0.493184 3.2:-44485404 None:intergenic
ACTTTGGAAATATCCTTCAA+TGG 0.495344 3.2:+44486924 MS.gene027072:CDS
GATGGCCGTCATATTGGCAT+TGG 0.495923 3.2:+44486253 MS.gene027072:CDS
ATATCCTTCAATGGTGAAGA+TGG 0.498891 3.2:+44486933 MS.gene027072:CDS
CGCACTACATGGTGACTGAA+GGG 0.507766 3.2:+44485358 MS.gene027072:CDS
CTGGAAGGAGAGCACACTTG+TGG 0.508404 3.2:-44485405 None:intergenic
AAGAAATCAACTGGCGGCGT+AGG 0.510142 3.2:-44485504 None:intergenic
ATGCGGCTCAAATGTGAAAA+CGG 0.515552 3.2:-44487114 None:intergenic
GGGCATGACCGAGAAGTTTG+CGG 0.518071 3.2:+44486574 MS.gene027072:CDS
AACAGTAACAAGTTCTGAAG+TGG 0.523430 3.2:-44486188 None:intergenic
CTGCAGTTGAGAACTTTGAA+AGG 0.529944 3.2:+44486427 MS.gene027072:intron
GAACTTTGAAAGGTGGGCAT+AGG 0.530523 3.2:+44486437 MS.gene027072:CDS
AATAACCACATACTCACAAC+CGG 0.531617 3.2:+44486484 MS.gene027072:CDS
TCCCTCCGGTTGTGAGTATG+TGG 0.533027 3.2:-44486489 None:intergenic
GCCCCGGCGAATTATTCCTC+AGG 0.533857 3.2:+44485578 MS.gene027072:CDS
CTCATTCTTGTTCCACAACA+GGG 0.537252 3.2:-44486857 None:intergenic
CTGCAGTAAAAGCTCTTGCT+TGG 0.539760 3.2:+44486713 MS.gene027072:CDS
ACACCTGAGGAATAATTCGC+CGG 0.542147 3.2:-44485581 None:intergenic
TGATAATCAACTTTACATCT+GGG 0.543770 3.2:+44486642 MS.gene027072:CDS
TTTCGATTATGCGCACTACA+TGG 0.544215 3.2:+44485347 MS.gene027072:CDS
TGCTTGAACTCAGTTGACAC+TGG 0.544299 3.2:+44486820 MS.gene027072:CDS
GACAGTGGCAACAGCTGCAG+CGG 0.545471 3.2:+44487014 MS.gene027072:CDS
AGGGAGCTACTCAGCTCTCA+TGG 0.545560 3.2:+44486880 MS.gene027072:CDS
TATTTGTGGGATGCATCAAA+CGG 0.551368 3.2:+44486163 MS.gene027072:CDS
AAATCAACTGGCGGCGTAGG+TGG 0.551699 3.2:-44485501 None:intergenic
CAACTCTGAAGTGCAGCTAT+GGG 0.552386 3.2:+44486282 MS.gene027072:CDS
TAATCCAATTGCAAGAACAT+TGG 0.554610 3.2:-44486128 None:intergenic
CTCACAACCGGAGGGATGGA+TGG 0.555824 3.2:+44486496 MS.gene027072:CDS
CATATGGCGCAGAGCCCAGA+TGG 0.555918 3.2:+44486988 MS.gene027072:CDS
GTGGGCATAGGCAGCGAGTA+GGG 0.557831 3.2:+44486449 MS.gene027072:CDS
CACCTGAGGAATAATTCGCC+GGG 0.559324 3.2:-44485580 None:intergenic
AGTTGAGAACTTTGAAAGGT+GGG 0.559397 3.2:+44486431 MS.gene027072:intron
TGGCGCAGAGCCCAGATGGT+TGG 0.562028 3.2:+44486992 MS.gene027072:CDS
TACCTCAATTTACTGGATTG+GGG 0.562441 3.2:+44486100 MS.gene027072:CDS
GCTAGTAAATTCCCTTGGAA+AGG 0.568517 3.2:-44486739 None:intergenic
GTCTACAATTGGCCAGTGGA+GGG 0.570881 3.2:+44486617 MS.gene027072:CDS
GTGAAGATGGCAGAACTCAA+TGG 0.571074 3.2:+44486946 MS.gene027072:CDS
GCGCACTACATGGTGACTGA+AGG 0.571364 3.2:+44485357 MS.gene027072:CDS
CATACTCACAACCGGAGGGA+TGG 0.571933 3.2:+44486492 MS.gene027072:CDS
CAGTTGAGAACTTTGAAAGG+TGG 0.572101 3.2:+44486430 MS.gene027072:intron
GGTCTACAATTGGCCAGTGG+AGG 0.575013 3.2:+44486616 MS.gene027072:CDS
GTTACTGTTGATGATGAAGA+TGG 0.576596 3.2:+44486202 MS.gene027072:CDS
TTCCCCAATCCAGTAAATTG+AGG 0.576674 3.2:-44486102 None:intergenic
TTCCTGTAAGCTTCTCTGGA+AGG 0.579286 3.2:-44485420 None:intergenic
ACTGAAGGGGCTAAAGCTAA+AGG 0.581037 3.2:+44485372 MS.gene027072:CDS
AGGGGCTAAAGCTAAAGGTA+AGG 0.581254 3.2:+44485377 MS.gene027072:CDS
TCTGTAAGTTGGGCTCCTGA+TGG 0.584539 3.2:+44486235 MS.gene027072:CDS
CCCAACTTACAGATGTGATA+GGG 0.585245 3.2:-44486225 None:intergenic
AATTCTACCATCCATCCCTC+CGG 0.585408 3.2:-44486503 None:intergenic
AAAAGCATTCCAAAACCTCA+AGG 0.587395 3.2:-44487043 None:intergenic
GGTGGGCATAGGCAGCGAGT+AGG 0.596714 3.2:+44486448 MS.gene027072:CDS
CATACCGAATAGATCAGCTA+TGG 0.601151 3.2:+44485323 MS.gene027072:CDS
AGCGAGTAGGGTCTTTGGCA+TGG 0.601597 3.2:+44486461 MS.gene027072:CDS
ATTGGATGCTCCAGACATTG+TGG 0.612151 3.2:+44486069 MS.gene027072:CDS
AAGATAAAACCATCAAGCCC+CGG 0.617625 3.2:+44485562 MS.gene027072:CDS
TGTTGTGGAACAAGAATGAG+AGG 0.618210 3.2:+44486860 MS.gene027072:CDS
GCTTGAACTCAGTTGACACT+GGG 0.627877 3.2:+44486821 MS.gene027072:CDS
CAGTAGCTAGTAAATTCCCT+TGG 0.629888 3.2:-44486744 None:intergenic
AATTTACTAGCTACTGGCGG+AGG 0.633586 3.2:+44486751 MS.gene027072:CDS
TCAAGCCAATGCCAATATGA+CGG 0.636859 3.2:-44486258 None:intergenic
GAGCCCAGATGGTTGGACAG+TGG 0.641101 3.2:+44486999 MS.gene027072:CDS
TCTGGTCTACAATTGGCCAG+TGG 0.642557 3.2:+44486613 MS.gene027072:CDS
ACCACATACTCACAACCGGA+GGG 0.650856 3.2:+44486488 MS.gene027072:CDS
GCACTACATGGTGACTGAAG+GGG 0.656801 3.2:+44485359 MS.gene027072:CDS
AGTTTGCGGATTGAAATGGT+CGG 0.668119 3.2:+44486588 MS.gene027072:CDS
GTTCAAGCATGCACCTGTGT+GGG 0.668846 3.2:-44486806 None:intergenic
CTGCAGCGGATGAAACCTTG+AGG 0.678181 3.2:+44487028 MS.gene027072:CDS
AGTTCAAGCATGCACCTGTG+TGG 0.678713 3.2:-44486807 None:intergenic
ACATTGTTGAAACATACAGA+GGG 0.688874 3.2:+44486554 MS.gene027072:CDS
AACCACATACTCACAACCGG+AGG 0.695066 3.2:+44486487 MS.gene027072:CDS
TGAGAGAAGAAATCAACTGG+CGG 0.700989 3.2:-44485510 None:intergenic
CACATTGTTGAAACATACAG+AGG 0.702329 3.2:+44486553 MS.gene027072:CDS
GGGATAGAAGTACTTCAACA+AGG 0.702662 3.2:+44486662 MS.gene027072:CDS
GGGAATTTACTAGCTACTGG+CGG 0.715639 3.2:+44486748 MS.gene027072:CDS
ATGTTACTACTTATCGAATG+CGG 0.717524 3.2:-44487131 None:intergenic
GTTGTGGAACAAGAATGAGA+GGG 0.727155 3.2:+44486861 MS.gene027072:CDS
ACCTGAGGAATAATTCGCCG+GGG 0.733838 3.2:-44485579 None:intergenic
ATAGAAGTACTTCAACAAGG+TGG 0.753913 3.2:+44486665 MS.gene027072:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATTAGAAAACTAGTAGT+AGG - chr3.2:44486392-44486411 None:intergenic 20.0%
!!! CACAAAATTGCAAAATATTT+TGG - chr3.2:44486008-44486027 None:intergenic 20.0%
!!! GATTTAAGAATTTTGAACTT+TGG + chr3.2:44485836-44485855 MS.gene027072:intron 20.0%
!!! TGTTTTCTTAATCGTTTTTA+TGG + chr3.2:44485759-44485778 MS.gene027072:intron 20.0%
! ATGATAATCAACTTTACATC+TGG + chr3.2:44486641-44486660 MS.gene027072:CDS 25.0%
! TGATAATCAACTTTACATCT+GGG + chr3.2:44486642-44486661 MS.gene027072:CDS 25.0%
!! CACAAAATTGCAAAATCTTT+TGG - chr3.2:44485901-44485920 None:intergenic 25.0%
!! GATTTGAGAATTTTGAACTT+CGG + chr3.2:44485943-44485962 MS.gene027072:intron 25.0%
!!! ATCAGTTTTTGAAAATCAAG+TGG + chr3.2:44485728-44485747 MS.gene027072:intron 25.0%
!!! TCAGTTTTTGAAAATCAAGT+GGG + chr3.2:44485729-44485748 MS.gene027072:intron 25.0%
!!! TTTTGTGCTAATTGATTTGT+TGG + chr3.2:44485914-44485933 MS.gene027072:intron 25.0%
AAATCCATAGCTGATCTATT+CGG - chr3.2:44485330-44485349 None:intergenic 30.0%
AATGTTCTTGCAATTGGATT+AGG + chr3.2:44486130-44486149 MS.gene027072:CDS 30.0%
ACATTGTTGAAACATACAGA+GGG + chr3.2:44486554-44486573 MS.gene027072:CDS 30.0%
AGGAAACACATTGTATTTGT+GGG + chr3.2:44486150-44486169 MS.gene027072:CDS 30.0%
ATAACGATGTATCGTTTGAA+GGG - chr3.2:44486349-44486368 None:intergenic 30.0%
ATTACCTCAATTTACTGGAT+TGG + chr3.2:44486098-44486117 MS.gene027072:CDS 30.0%
TAATCCAATTGCAAGAACAT+TGG - chr3.2:44486131-44486150 None:intergenic 30.0%
TAGGAAACACATTGTATTTG+TGG + chr3.2:44486149-44486168 MS.gene027072:CDS 30.0%
TGACTATTACCTCAATTTAC+TGG + chr3.2:44486093-44486112 MS.gene027072:CDS 30.0%
TTACCTCAATTTACTGGATT+GGG + chr3.2:44486099-44486118 MS.gene027072:CDS 30.0%
! ACTTTGGAAATATCCTTCAA+TGG + chr3.2:44486924-44486943 MS.gene027072:CDS 30.0%
!! AGACAGGTTTTATACTTATC+TGG + chr3.2:44486319-44486338 MS.gene027072:intron 30.0%
!! TCTTATCTGAAAGTGTTGAA+TGG + chr3.2:44485976-44485995 MS.gene027072:intron 30.0%
!! TCTTGTGCTAATTGATTTGT+TGG + chr3.2:44485807-44485826 MS.gene027072:intron 30.0%
AATAACCACATACTCACAAC+CGG + chr3.2:44486484-44486503 MS.gene027072:CDS 35.0%
ATATCCTTCAATGGTGAAGA+TGG + chr3.2:44486933-44486952 MS.gene027072:CDS 35.0%
ATTTCAATCCGCAAACTTCT+CGG - chr3.2:44486585-44486604 None:intergenic 35.0%
CACAGAATCAGATAACACTT+TGG + chr3.2:44486908-44486927 MS.gene027072:CDS 35.0%
CACATTGTTGAAACATACAG+AGG + chr3.2:44486553-44486572 MS.gene027072:CDS 35.0%
CACTTCAAGAGTTCTTCATA+TGG + chr3.2:44486972-44486991 MS.gene027072:CDS 35.0%
CATAACGATGTATCGTTTGA+AGG - chr3.2:44486350-44486369 None:intergenic 35.0%
GTTCTTGAAAGCTAGAATTC+TGG - chr3.2:44485478-44485497 None:intergenic 35.0%
TAATAGTCATCCACAATGTC+TGG - chr3.2:44486082-44486101 None:intergenic 35.0%
TACCTCAATTTACTGGATTG+GGG + chr3.2:44486100-44486119 MS.gene027072:CDS 35.0%
TATTTGTGGGATGCATCAAA+CGG + chr3.2:44486163-44486182 MS.gene027072:CDS 35.0%
TCATGAGAGAAGAAATCAAC+TGG - chr3.2:44485516-44485535 None:intergenic 35.0%
! AGTTGAGAACTTTGAAAGGT+GGG + chr3.2:44486431-44486450 MS.gene027072:intron 35.0%
! TGCATGAACTCTAGTTCAAA+CGG + chr3.2:44485675-44485694 MS.gene027072:intron 35.0%
!! AAAAGCATTCCAAAACCTCA+AGG - chr3.2:44487046-44487065 None:intergenic 35.0%
!! AACAGTAACAAGTTCTGAAG+TGG - chr3.2:44486191-44486210 None:intergenic 35.0%
!! ATAGAAGTACTTCAACAAGG+TGG + chr3.2:44486665-44486684 MS.gene027072:CDS 35.0%
!! GTTACTGTTGATGATGAAGA+TGG + chr3.2:44486202-44486221 MS.gene027072:CDS 35.0%
!!! TTGAGGTTTTGGAATGCTTT+TGG + chr3.2:44487045-44487064 MS.gene027072:CDS 35.0%
AAGATAAAACCATCAAGCCC+CGG + chr3.2:44485562-44485581 MS.gene027072:CDS 40.0%
ATGCGGCTCAAATGTGAAAA+CGG - chr3.2:44487117-44487136 None:intergenic 40.0%
CAAGGGAATTTACTAGCTAC+TGG + chr3.2:44486745-44486764 MS.gene027072:CDS 40.0%
CAGTAGCTAGTAAATTCCCT+TGG - chr3.2:44486747-44486766 None:intergenic 40.0%
CATACCGAATAGATCAGCTA+TGG + chr3.2:44485323-44485342 MS.gene027072:CDS 40.0%
CCCAACTTACAGATGTGATA+GGG - chr3.2:44486228-44486247 None:intergenic 40.0%
CCCTATCACATCTGTAAGTT+GGG + chr3.2:44486225-44486244 MS.gene027072:CDS 40.0%
CTAGTTCAAACGGCAAAAGT+CGG + chr3.2:44485685-44485704 MS.gene027072:intron 40.0%
CTCAACTGCAGAAACATAAC+AGG - chr3.2:44486418-44486437 None:intergenic 40.0%
CTCATTCTTGTTCCACAACA+GGG - chr3.2:44486860-44486879 None:intergenic 40.0%
CTGCAGTTGAGAACTTTGAA+AGG + chr3.2:44486427-44486446 MS.gene027072:intron 40.0%
GCTAGTAAATTCCCTTGGAA+AGG - chr3.2:44486742-44486761 None:intergenic 40.0%
GTGGGTATTCCAAAACTTGA+TGG - chr3.2:44486791-44486810 None:intergenic 40.0%
GTTGTGGAACAAGAATGAGA+GGG + chr3.2:44486861-44486880 MS.gene027072:CDS 40.0%
TCAAGCCAATGCCAATATGA+CGG - chr3.2:44486261-44486280 None:intergenic 40.0%
TCTCATTCTTGTTCCACAAC+AGG - chr3.2:44486861-44486880 None:intergenic 40.0%
TGAGAGAAGAAATCAACTGG+CGG - chr3.2:44485513-44485532 None:intergenic 40.0%
TGTTGTGGAACAAGAATGAG+AGG + chr3.2:44486860-44486879 MS.gene027072:CDS 40.0%
TTCCCCAATCCAGTAAATTG+AGG - chr3.2:44486105-44486124 None:intergenic 40.0%
TTGATTATCATTCCCTCCAC+TGG - chr3.2:44486632-44486651 None:intergenic 40.0%
TTTCGATTATGCGCACTACA+TGG + chr3.2:44485347-44485366 MS.gene027072:CDS 40.0%
! AGTTTGCGGATTGAAATGGT+CGG + chr3.2:44486588-44486607 MS.gene027072:CDS 40.0%
! CAGTTGAGAACTTTGAAAGG+TGG + chr3.2:44486430-44486449 MS.gene027072:intron 40.0%
! GAGAAGTTTGCGGATTGAAA+TGG + chr3.2:44486584-44486603 MS.gene027072:CDS 40.0%
! GCGATGAAACCATCAAGTTT+TGG + chr3.2:44486779-44486798 MS.gene027072:CDS 40.0%
!! CAGAAGCTCTAAACATGAAC+AGG + chr3.2:44485451-44485470 MS.gene027072:CDS 40.0%
!! GGGATAGAAGTACTTCAACA+AGG + chr3.2:44486662-44486681 MS.gene027072:CDS 40.0%
!! TTGTTTTGCAGACTTCTGAG+AGG + chr3.2:44486044-44486063 MS.gene027072:intron 40.0%
!!! AAGTTTTGGAATACCCACAC+AGG + chr3.2:44486793-44486812 MS.gene027072:CDS 40.0%
AATTCTACCATCCATCCCTC+CGG - chr3.2:44486506-44486525 None:intergenic 45.0%
AATTTACTAGCTACTGGCGG+AGG + chr3.2:44486751-44486770 MS.gene027072:CDS 45.0%
ACACCTGAGGAATAATTCGC+CGG - chr3.2:44485584-44485603 None:intergenic 45.0%
ACTGAAGGGGCTAAAGCTAA+AGG + chr3.2:44485372-44485391 MS.gene027072:CDS 45.0%
AGCGCCAATGTTCTTGCAAT+TGG + chr3.2:44486124-44486143 MS.gene027072:CDS 45.0%
AGGGGCTAAAGCTAAAGGTA+AGG + chr3.2:44485377-44485396 MS.gene027072:CDS 45.0%
ATTGGATGCTCCAGACATTG+TGG + chr3.2:44486069-44486088 MS.gene027072:CDS 45.0%
CTGCAGTAAAAGCTCTTGCT+TGG + chr3.2:44486713-44486732 MS.gene027072:CDS 45.0%
GCCCAACTTACAGATGTGAT+AGG - chr3.2:44486229-44486248 None:intergenic 45.0%
GCCCTATCACATCTGTAAGT+TGG + chr3.2:44486224-44486243 MS.gene027072:CDS 45.0%
GCTTGAACTCAGTTGACACT+GGG + chr3.2:44486821-44486840 MS.gene027072:CDS 45.0%
GGATACAGCTTCAGATAGAC+AGG + chr3.2:44486303-44486322 MS.gene027072:CDS 45.0%
GGGAATTTACTAGCTACTGG+CGG + chr3.2:44486748-44486767 MS.gene027072:CDS 45.0%
GTGAAGATGGCAGAACTCAA+TGG + chr3.2:44486946-44486965 MS.gene027072:CDS 45.0%
TAGCTTCCTGTAAGCTTCTC+TGG - chr3.2:44485427-44485446 None:intergenic 45.0%
TCTGCCATCTTCACCATTGA+AGG - chr3.2:44486940-44486959 None:intergenic 45.0%
TGCTTGAACTCAGTTGACAC+TGG + chr3.2:44486820-44486839 MS.gene027072:CDS 45.0%
TTCCTGTAAGCTTCTCTGGA+AGG - chr3.2:44485423-44485442 None:intergenic 45.0%
! CGGATGAAACCTTGAGGTTT+TGG + chr3.2:44487034-44487053 MS.gene027072:CDS 45.0%
! GAACTTTGAAAGGTGGGCAT+AGG + chr3.2:44486437-44486456 MS.gene027072:CDS 45.0%
! GCAGACTTCTGAGAGGATAT+TGG + chr3.2:44486051-44486070 MS.gene027072:intron 45.0%
!! ACAACTCTGAAGTGCAGCTA+TGG + chr3.2:44486281-44486300 MS.gene027072:CDS 45.0%
!! CAACTCTGAAGTGCAGCTAT+GGG + chr3.2:44486282-44486301 MS.gene027072:CDS 45.0%
!! GCAGCTTTTGTTGCTACTTC+TGG - chr3.2:44487075-44487094 None:intergenic 45.0%
!! TTGCTTGGTGTCCTTTCCAA+GGG + chr3.2:44486728-44486747 MS.gene027072:CDS 45.0%
!!! ATATTTTATCATTATTATTT+TGG + chr3.2:44485651-44485670 MS.gene027072:intron 5.0%
AACCACATACTCACAACCGG+AGG + chr3.2:44486487-44486506 MS.gene027072:CDS 50.0%
AAGAAATCAACTGGCGGCGT+AGG - chr3.2:44485507-44485526 None:intergenic 50.0%
AATAATTCGCCGGGGCTTGA+TGG - chr3.2:44485574-44485593 None:intergenic 50.0%
ACCACATACTCACAACCGGA+GGG + chr3.2:44486488-44486507 MS.gene027072:CDS 50.0%
ACCTGAGGAATAATTCGCCG+GGG - chr3.2:44485582-44485601 None:intergenic 50.0%
AGTTCAAGCATGCACCTGTG+TGG - chr3.2:44486810-44486829 None:intergenic 50.0%
ATGCCAATATGACGGCCATC+AGG - chr3.2:44486253-44486272 None:intergenic 50.0%
CACCTGAGGAATAATTCGCC+GGG - chr3.2:44485583-44485602 None:intergenic 50.0%
CGCACTACATGGTGACTGAA+GGG + chr3.2:44485358-44485377 MS.gene027072:CDS 50.0%
CTCAGGTATGTTCCCTGTTG+TGG + chr3.2:44486845-44486864 MS.gene027072:CDS 50.0%
CTCCTTCCAGAGAAGCTTAC+AGG + chr3.2:44485418-44485437 MS.gene027072:CDS 50.0%
CTTCAACAAGGTGGCTTCAC+AGG + chr3.2:44486674-44486693 MS.gene027072:CDS 50.0%
GATGGCCGTCATATTGGCAT+TGG + chr3.2:44486253-44486272 MS.gene027072:CDS 50.0%
GCACTACATGGTGACTGAAG+GGG + chr3.2:44485359-44485378 MS.gene027072:CDS 50.0%
GTCTACAATTGGCCAGTGGA+GGG + chr3.2:44486617-44486636 MS.gene027072:CDS 50.0%
GTTCAAGCATGCACCTGTGT+GGG - chr3.2:44486809-44486828 None:intergenic 50.0%
TCTGGTCTACAATTGGCCAG+TGG + chr3.2:44486613-44486632 MS.gene027072:CDS 50.0%
TTGCCACTGTCCAACCATCT+GGG - chr3.2:44487005-44487024 None:intergenic 50.0%
! AACGGCTCAGCTCTTGCTTT+TGG - chr3.2:44487099-44487118 None:intergenic 50.0%
! GGATTGAAATGGTCGGCTTC+TGG + chr3.2:44486595-44486614 MS.gene027072:CDS 50.0%
! GTCGGCTTCTGGTCTACAAT+TGG + chr3.2:44486606-44486625 MS.gene027072:CDS 50.0%
! TCTGTAAGTTGGGCTCCTGA+TGG + chr3.2:44486235-44486254 MS.gene027072:CDS 50.0%
! TGGAAGGAGAGCACACTTGT+GGG - chr3.2:44485407-44485426 None:intergenic 50.0%
!! CTTGCTTGGTGTCCTTTCCA+AGG + chr3.2:44486727-44486746 MS.gene027072:CDS 50.0%
AAATCAACTGGCGGCGTAGG+TGG - chr3.2:44485504-44485523 None:intergenic 55.0%
AGCGAGTAGGGTCTTTGGCA+TGG + chr3.2:44486461-44486480 MS.gene027072:CDS 55.0%
AGGGAGCTACTCAGCTCTCA+TGG + chr3.2:44486880-44486899 MS.gene027072:CDS 55.0%
CACACACACACACACACCTG+AGG - chr3.2:44485597-44485616 None:intergenic 55.0%
CATACTCACAACCGGAGGGA+TGG + chr3.2:44486492-44486511 MS.gene027072:CDS 55.0%
CTCAGTTGACACTGGGTCTC+AGG + chr3.2:44486828-44486847 MS.gene027072:CDS 55.0%
CTGCAGCGGATGAAACCTTG+AGG + chr3.2:44487028-44487047 MS.gene027072:CDS 55.0%
GCGCACTACATGGTGACTGA+AGG + chr3.2:44485357-44485376 MS.gene027072:CDS 55.0%
GCTCCTGATGGCCGTCATAT+TGG + chr3.2:44486247-44486266 MS.gene027072:CDS 55.0%
GGGCATGACCGAGAAGTTTG+CGG + chr3.2:44486574-44486593 MS.gene027072:CDS 55.0%
GGTCTACAATTGGCCAGTGG+AGG + chr3.2:44486616-44486635 MS.gene027072:CDS 55.0%
GTTGCCACTGTCCAACCATC+TGG - chr3.2:44487006-44487025 None:intergenic 55.0%
TAGGCAGCGAGTAGGGTCTT+TGG + chr3.2:44486456-44486475 MS.gene027072:CDS 55.0%
TCCCTCCGGTTGTGAGTATG+TGG - chr3.2:44486492-44486511 None:intergenic 55.0%
! CTGGAAGGAGAGCACACTTG+TGG - chr3.2:44485408-44485427 None:intergenic 55.0%
CATATGGCGCAGAGCCCAGA+TGG + chr3.2:44486988-44487007 MS.gene027072:CDS 60.0%
CTCACAACCGGAGGGATGGA+TGG + chr3.2:44486496-44486515 MS.gene027072:CDS 60.0%
GACAGTGGCAACAGCTGCAG+CGG + chr3.2:44487014-44487033 MS.gene027072:CDS 60.0%
GAGCCCAGATGGTTGGACAG+TGG + chr3.2:44486999-44487018 MS.gene027072:CDS 60.0%
GCCCCGGCGAATTATTCCTC+AGG + chr3.2:44485578-44485597 MS.gene027072:CDS 60.0%
GTGGGCATAGGCAGCGAGTA+GGG + chr3.2:44486449-44486468 MS.gene027072:CDS 60.0%
CTAGCTACTGGCGGAGGCAG+TGG + chr3.2:44486757-44486776 MS.gene027072:CDS 65.0%
GGTGGGCATAGGCAGCGAGT+AGG + chr3.2:44486448-44486467 MS.gene027072:CDS 65.0%
TGGCGCAGAGCCCAGATGGT+TGG + chr3.2:44486992-44487011 MS.gene027072:CDS 65.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 44485312 44487143 44485312 ID=MS.gene027072
chr3.2 mRNA 44485312 44487143 44485312 ID=MS.gene027072.t1;Parent=MS.gene027072
chr3.2 exon 44485312 44485599 44485312 ID=MS.gene027072.t1.exon1;Parent=MS.gene027072.t1
chr3.2 CDS 44485312 44485599 44485312 ID=cds.MS.gene027072.t1;Parent=MS.gene027072.t1
chr3.2 exon 44486055 44486324 44486055 ID=MS.gene027072.t1.exon2;Parent=MS.gene027072.t1
chr3.2 CDS 44486055 44486324 44486055 ID=cds.MS.gene027072.t1;Parent=MS.gene027072.t1
chr3.2 exon 44486433 44487143 44486433 ID=MS.gene027072.t1.exon3;Parent=MS.gene027072.t1
chr3.2 CDS 44486433 44487143 44486433 ID=cds.MS.gene027072.t1;Parent=MS.gene027072.t1
Gene Sequence

>MS.gene027072

TTGGATAGATTCATACCGAATAGATCAGCTATGGATTTCGATTATGCGCACTACATGGTGACTGAAGGGGCTAAAGCTAAAGGTAAGGAAAACCCACAAGTGTGCTCTCCTTCCAGAGAAGCTTACAGGAAGCTACTTGCAGAAGCTCTAAACATGAACAGGACCAGAATTCTAGCTTTCAAGAACAAGCCACCTACGCCGCCAGTTGATTTCTTCTCTCATGATATCATTACTTCATCAACTCTTCGACAAGATAAAACCATCAAGCCCCGGCGAATTATTCCTCAGGTGTGTGTGTGTGTGTGTGTGTTTCTTAATGATATCTGATTCATATTTCATATATTTTATCATTATTATTTTGGTTGCATGAACTCTAGTTCAAACGGCAAAAGTCGGATTTTAAAATTTTGCACTTGATCAGTTTTTGAAAATCAAGTGGGTCTGAATTGTTTTCTTAATCGTTTTTATGGTGTGTGAAATCACAATAGTTTGCAATCTTGTGCTAATTGATTTGTTGGTTATCTGATTTAAGAATTTTGAACTTTGGTTTTCTTAATTCTTATCTGAAAGTGTTGTATGTTGTAAACCAAAAGATTTTGCAATTTTGTGCTAATTGATTTGTTGGTTATCTGATTTGAGAATTTTGAACTTCGGTTTTCTTAATTCTTATCTGAAAGTGTTGAATGGTGTAAACCAAAATATTTTGCAATTTTGTGCTAATTGATTGATTTGTTGTTTTGCAGACTTCTGAGAGGATATTGGATGCTCCAGACATTGTGGATGACTATTACCTCAATTTACTGGATTGGGGAAGCGCCAATGTTCTTGCAATTGGATTAGGAAACACATTGTATTTGTGGGATGCATCAAACGGTTCCACTTCAGAACTTGTTACTGTTGATGATGAAGATGGCCCTATCACATCTGTAAGTTGGGCTCCTGATGGCCGTCATATTGGCATTGGCTTGAACAACTCTGAAGTGCAGCTATGGGATACAGCTTCAGATAGACAGGTTTTATACTTATCTGGTTTACCCTTCAAACGATACATCGTTATGAGCTATTGAGTATATAATTCCTACTACTAGTTTTCTAATATTATTCCTGTTATGTTTCTGCAGTTGAGAACTTTGAAAGGTGGGCATAGGCAGCGAGTAGGGTCTTTGGCATGGAATAACCACATACTCACAACCGGAGGGATGGATGGTAGAATTATCAATAATGATGTAAGAATTAGAGCACACATTGTTGAAACATACAGAGGGCATGACCGAGAAGTTTGCGGATTGAAATGGTCGGCTTCTGGTCTACAATTGGCCAGTGGAGGGAATGATAATCAACTTTACATCTGGGATAGAAGTACTTCAACAAGGTGGCTTCACAGGCTTGAAGATCATACATCTGCAGTAAAAGCTCTTGCTTGGTGTCCTTTCCAAGGGAATTTACTAGCTACTGGCGGAGGCAGTGGCGATGAAACCATCAAGTTTTGGAATACCCACACAGGTGCATGCTTGAACTCAGTTGACACTGGGTCTCAGGTATGTTCCCTGTTGTGGAACAAGAATGAGAGGGAGCTACTCAGCTCTCATGGTTTTACACAGAATCAGATAACACTTTGGAAATATCCTTCAATGGTGAAGATGGCAGAACTCAATGGTCACACTTCAAGAGTTCTTCATATGGCGCAGAGCCCAGATGGTTGGACAGTGGCAACAGCTGCAGCGGATGAAACCTTGAGGTTTTGGAATGCTTTTGGCACTCCAGAAGTAGCAACAAAAGCTGCACCAAAAGCAAGAGCTGAGCCGTTTTCACATTTGAGCCGCATTCGATAA
Protein sequence

>MS.gene027072.t1

LDRFIPNRSAMDFDYAHYMVTEGAKAKGKENPQVCSPSREAYRKLLAEALNMNRTRILAFKNKPPTPPVDFFSHDIITSSTLRQDKTIKPRRIIPQTSERILDAPDIVDDYYLNLLDWGSANVLAIGLGNTLYLWDASNGSTSELVTVDDEDGPITSVSWAPDGRHIGIGLNNSEVQLWDTASDRQLRTLKGGHRQRVGSLAWNNHILTTGGMDGRIINNDVRIRAHIVETYRGHDREVCGLKWSASGLQLASGGNDNQLYIWDRSTSTRWLHRLEDHTSAVKALAWCPFQGNLLATGGGSGDETIKFWNTHTGACLNSVDTGSQVCSLLWNKNERELLSSHGFTQNQITLWKYPSMVKMAELNGHTSRVLHMAQSPDGWTVATAAADETLRFWNAFGTPEVATKAAPKARAEPFSHLSRIR