AlfalfaGEDB | MS.gene21888

M. sativa cultivar XinJiangDaYe / MS.gene21888

KEGG: K03363
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00400.35 PF12894.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21888.t1	XP_003610876.1	99.1	454	4	0	1	454	1	454	1.50E-265	924.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21888.t1	Q9SZA4	79.8	450	89	2	7	454	8	457	2.4e-216	752.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21888.t1	G7K6J6	99.1	454	4	0	1	454	1	454	1.1e-265	924.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049298	MS.gene21888	0.85852	7.79E-63	-1.69E-46
MS.gene050707	MS.gene21888	0.81774	2.82E-52	-1.69E-46
MS.gene050875	MS.gene21888	0.812538	4.03E-51	-1.69E-46
MS.gene051816	MS.gene21888	0.811519	6.72E-51	-1.69E-46
MS.gene051896	MS.gene21888	0.829572	4.78E-55	-1.69E-46
MS.gene051914	MS.gene21888	0.864988	8.18E-65	-1.69E-46
MS.gene051955	MS.gene21888	0.836073	1.16E-56	-1.69E-46
MS.gene052143	MS.gene21888	0.806393	8.41E-50	-1.69E-46
MS.gene052209	MS.gene21888	0.813205	2.88E-51	-1.69E-46
MS.gene052308	MS.gene21888	0.904362	1.33E-79	-1.69E-46
MS.gene052367	MS.gene21888	0.83636	9.79E-57	-1.69E-46
MS.gene053173	MS.gene21888	0.867757	1.08E-65	-1.69E-46
MS.gene053348	MS.gene21888	0.876378	1.46E-68	-1.69E-46
MS.gene054551	MS.gene21888	0.892286	1.83E-74	-1.69E-46
MS.gene054552	MS.gene21888	0.824799	6.64E-54	-1.69E-46
MS.gene054593	MS.gene21888	0.893375	6.66E-75	-1.69E-46
MS.gene054633	MS.gene21888	0.856002	4.32E-62	-1.69E-46
MS.gene054662	MS.gene21888	0.847008	1.52E-59	-1.69E-46
MS.gene054850	MS.gene21888	0.829295	5.58E-55	-1.69E-46
MS.gene054885	MS.gene21888	0.826771	2.26E-54	-1.69E-46
MS.gene054941	MS.gene21888	0.862988	3.43E-64	-1.69E-46
MS.gene055308	MS.gene21888	0.90446	1.20E-79	-1.69E-46
MS.gene055578	MS.gene21888	0.879557	1.13E-69	-1.69E-46
MS.gene055579	MS.gene21888	0.819171	1.34E-52	-1.69E-46
MS.gene055957	MS.gene21888	0.854697	1.04E-61	-1.69E-46
MS.gene056041	MS.gene21888	0.88577	6.11E-72	-1.69E-46
MS.gene056042	MS.gene21888	0.822835	1.92E-53	-1.69E-46
MS.gene05640	MS.gene21888	0.90401	1.92E-79	-1.69E-46
MS.gene056526	MS.gene21888	0.812528	4.05E-51	-1.69E-46
MS.gene056528	MS.gene21888	0.863189	2.97E-64	-1.69E-46
MS.gene05652	MS.gene21888	0.895186	1.22E-75	-1.69E-46
MS.gene056618	MS.gene21888	0.830686	2.55E-55	-1.69E-46
MS.gene057101	MS.gene21888	0.84983	2.51E-60	-1.69E-46
MS.gene057367	MS.gene21888	0.858364	8.67E-63	-1.69E-46
MS.gene057458	MS.gene21888	0.846575	1.99E-59	-1.69E-46
MS.gene057669	MS.gene21888	0.853262	2.68E-61	-1.69E-46
MS.gene058013	MS.gene21888	0.833863	4.18E-56	-1.69E-46
MS.gene058128	MS.gene21888	0.97099	1.83E-132	-1.69E-46
MS.gene058827	MS.gene21888	0.805561	1.26E-49	-1.69E-46
MS.gene058828	MS.gene21888	0.800677	1.29E-48	-1.69E-46
MS.gene058853	MS.gene21888	0.929836	3.92E-93	-1.69E-46
MS.gene05926	MS.gene21888	0.811645	6.31E-51	-1.69E-46
MS.gene059553	MS.gene21888	0.859797	3.23E-63	-1.69E-46
MS.gene059616	MS.gene21888	0.893987	3.76E-75	-1.69E-46
MS.gene059905	MS.gene21888	0.836731	7.88E-57	-1.69E-46
MS.gene060473	MS.gene21888	0.841561	4.41E-58	-1.69E-46
MS.gene060565	MS.gene21888	0.823061	1.70E-53	-1.69E-46
MS.gene06093	MS.gene21888	0.879525	1.16E-69	-1.69E-46
MS.gene060985	MS.gene21888	0.80686	6.70E-50	-1.69E-46
MS.gene061099	MS.gene21888	0.879536	1.15E-69	-1.69E-46
MS.gene061260	MS.gene21888	0.842394	2.66E-58	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene045937	MS.gene21888	PPI
MS.gene23852	MS.gene21888	PPI
MS.gene69966	MS.gene21888	PPI
MS.gene97943	MS.gene21888	PPI
MS.gene071113	MS.gene21888	PPI
MS.gene21888	MS.gene62819	PPI
MS.gene027071	MS.gene21888	PPI
MS.gene67157	MS.gene21888	PPI
MS.gene69396	MS.gene21888	PPI
MS.gene21888	MS.gene65775	PPI
MS.gene21888	MS.gene80163	PPI
MS.gene012203	MS.gene21888	PPI
MS.gene21888	MS.gene29842	PPI
MS.gene022109	MS.gene21888	PPI
MS.gene21888	MS.gene033401	PPI
MS.gene34651	MS.gene21888	PPI
MS.gene014931	MS.gene21888	PPI
MS.gene010910	MS.gene21888	PPI
MS.gene052188	MS.gene21888	PPI
MS.gene025229	MS.gene21888	PPI
MS.gene045133	MS.gene21888	PPI
MS.gene063222	MS.gene21888	PPI
MS.gene25515	MS.gene21888	PPI
MS.gene060495	MS.gene21888	PPI
MS.gene21888	MS.gene06177	PPI
MS.gene21888	MS.gene51278	PPI
MS.gene07706	MS.gene21888	PPI
MS.gene027074	MS.gene21888	PPI
MS.gene069995	MS.gene21888	PPI
MS.gene21888	MS.gene87218	PPI
MS.gene062662	MS.gene21888	PPI
MS.gene20160	MS.gene21888	PPI
MS.gene69687	MS.gene21888	PPI
MS.gene21888	MS.gene014321	PPI
MS.gene21888	MS.gene064862	PPI
MS.gene21888	MS.gene23881	PPI
MS.gene23811	MS.gene21888	PPI
MS.gene51258	MS.gene21888	PPI
MS.gene23810	MS.gene21888	PPI
MS.gene46611	MS.gene21888	PPI
MS.gene82021	MS.gene21888	PPI
MS.gene024753	MS.gene21888	PPI
MS.gene81658	MS.gene21888	PPI
MS.gene84465	MS.gene21888	PPI
MS.gene78281	MS.gene21888	PPI
MS.gene55882	MS.gene21888	PPI
MS.gene21888	MS.gene30177	PPI
MS.gene21888	MS.gene84466	PPI
MS.gene037950	MS.gene21888	PPI
MS.gene70008	MS.gene21888	PPI
MS.gene060496	MS.gene21888	PPI
MS.gene64441	MS.gene21888	PPI
MS.gene24806	MS.gene21888	PPI
MS.gene004844	MS.gene21888	PPI
MS.gene52355	MS.gene21888	PPI
MS.gene30176	MS.gene21888	PPI
MS.gene24951	MS.gene21888	PPI
MS.gene21888	MS.gene027072	PPI
MS.gene066464	MS.gene21888	PPI
MS.gene21888	MS.gene65463	PPI
MS.gene20161	MS.gene21888	PPI
MS.gene51262	MS.gene21888	PPI
MS.gene050270	MS.gene21888	PPI
MS.gene24922	MS.gene21888	PPI
MS.gene027073	MS.gene21888	PPI
MS.gene33406	MS.gene21888	PPI
MS.gene21888	MS.gene067705	PPI
MS.gene010912	MS.gene21888	PPI
MS.gene050457	MS.gene21888	PPI
MS.gene21888	MS.gene37226	PPI
MS.gene75059	MS.gene21888	PPI
MS.gene34712	MS.gene21888	PPI
MS.gene92674	MS.gene21888	PPI
MS.gene51335	MS.gene21888	PPI
MS.gene21888	MS.gene99973	PPI
MS.gene21888	MS.gene30174	PPI
MS.gene027441	MS.gene21888	PPI
MS.gene81663	MS.gene21888	PPI
MS.gene21888	MS.gene008833	PPI
MS.gene21888	MS.gene064435	PPI
MS.gene068249	MS.gene21888	PPI
MS.gene81660	MS.gene21888	PPI
MS.gene066046	MS.gene21888	PPI
MS.gene41291	MS.gene21888	PPI
MS.gene21888	MS.gene48655	PPI
MS.gene27223	MS.gene21888	PPI
MS.gene66342	MS.gene21888	PPI
MS.gene038519	MS.gene21888	PPI
MS.gene050272	MS.gene21888	PPI
MS.gene06176	MS.gene21888	PPI
MS.gene030294	MS.gene21888	PPI
MS.gene014419	MS.gene21888	PPI
MS.gene91851	MS.gene21888	PPI
MS.gene29768	MS.gene21888	PPI
MS.gene039682	MS.gene21888	PPI
MS.gene043742	MS.gene21888	PPI
MS.gene21888	MS.gene037184	PPI
MS.gene022502	MS.gene21888	PPI
MS.gene85534	MS.gene21888	PPI
MS.gene042783	MS.gene21888	PPI
MS.gene066467	MS.gene21888	PPI
MS.gene82020	MS.gene21888	PPI
MS.gene21734	MS.gene21888	PPI
MS.gene66343	MS.gene21888	PPI
MS.gene33377	MS.gene21888	PPI
MS.gene71204	MS.gene21888	PPI
MS.gene064035	MS.gene21888	PPI
MS.gene77885	MS.gene21888	PPI
MS.gene21888	MS.gene26021	PPI
MS.gene26672	MS.gene21888	PPI
MS.gene36842	MS.gene21888	PPI
MS.gene97379	MS.gene21888	PPI
MS.gene99554	MS.gene21888	PPI
MS.gene90626	MS.gene21888	PPI
MS.gene050456	MS.gene21888	PPI
MS.gene42253	MS.gene21888	PPI
MS.gene21888	MS.gene32723	PPI
MS.gene058128	MS.gene21888	PPI
MS.gene99588	MS.gene21888	PPI
MS.gene027075	MS.gene21888	PPI
MS.gene21888	MS.gene053363	PPI
MS.gene011448	MS.gene21888	PPI
MS.gene066501	MS.gene21888	PPI
MS.gene25244	MS.gene21888	PPI
MS.gene068273	MS.gene21888	PPI
MS.gene21888	MS.gene75493	PPI
MS.gene63085	MS.gene21888	PPI
MS.gene55883	MS.gene21888	PPI
MS.gene056455	MS.gene21888	PPI
MS.gene21888	MS.gene32726	PPI
MS.gene21888	MS.gene71122	PPI
MS.gene89986	MS.gene21888	PPI
MS.gene26019	MS.gene21888	PPI
MS.gene30174	MS.gene21888	PPI
MS.gene064762	MS.gene21888	PPI
MS.gene052956	MS.gene21888	PPI
MS.gene049022	MS.gene21888	PPI
MS.gene91775	MS.gene21888	PPI
MS.gene35613	MS.gene21888	PPI
MS.gene30177	MS.gene21888	PPI
MS.gene98540	MS.gene21888	PPI
MS.gene69965	MS.gene21888	PPI
MS.gene066500	MS.gene21888	PPI
MS.gene067321	MS.gene21888	PPI
MS.gene82740	MS.gene21888	PPI
MS.gene049021	MS.gene21888	PPI
MS.gene30172	MS.gene21888	PPI
MS.gene067322	MS.gene21888	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21888.t1	MTR_5g008010	99.119	454	4	0	1	454	1	454	0.0	937
MS.gene21888.t1	MTR_3g051690	91.503	459	34	4	1	454	1	459	0.0	872
MS.gene21888.t1	MTR_7g106870	75.223	448	105	5	12	454	11	457	0.0	707
MS.gene21888.t1	MTR_7g451830	66.966	445	126	7	15	454	3	431	0.0	591
MS.gene21888.t1	MTR_7g047640	66.966	445	126	7	15	454	3	431	0.0	591
MS.gene21888.t1	MTR_7g057540	65.011	443	135	7	14	454	2	426	0.0	563
MS.gene21888.t1	MTR_7g055590	64.932	442	135	7	14	453	2	425	0.0	561
MS.gene21888.t1	MTR_7g053100	61.607	448	147	8	17	454	4	436	0.0	537
MS.gene21888.t1	MTR_7g034625	71.160	319	90	1	124	440	13	331	1.14e-172	488
MS.gene21888.t1	MTR_2g100540	51.945	437	160	13	25	454	4	397	2.69e-135	395
MS.gene21888.t1	MTR_2g100460	56.322	348	133	6	107	452	52	382	2.03e-129	380
MS.gene21888.t1	MTR_2g060710	45.388	412	202	8	33	430	26	428	9.05e-123	365
MS.gene21888.t1	MTR_3g067940	45.012	411	201	7	33	429	57	456	2.79e-115	347
MS.gene21888.t1	MTR_4g102510	41.611	447	218	9	11	433	29	456	4.74e-113	341
MS.gene21888.t1	MTR_2g012630	27.386	241	156	9	140	374	18	245	1.42e-17	86.3
MS.gene21888.t1	MTR_2g012630	27.386	241	156	9	140	374	18	245	1.61e-17	85.9
MS.gene21888.t1	MTR_4g415070	61.111	54	21	0	379	432	57	110	6.98e-16	79.0
MS.gene21888.t1	MTR_6g004040	26.891	238	155	9	140	371	19	243	3.61e-15	78.6
MS.gene21888.t1	MTR_5g016370	23.630	292	162	8	143	426	312	550	8.55e-15	77.0
MS.gene21888.t1	MTR_4g095058	26.336	262	168	11	177	427	287	534	2.81e-14	75.5
MS.gene21888.t1	MTR_4g095058	26.336	262	168	11	177	427	285	532	3.23e-14	75.1
MS.gene21888.t1	MTR_1g054300	27.778	252	152	11	182	426	253	481	3.68e-14	74.7
MS.gene21888.t1	MTR_1g054300	25.510	294	156	10	182	428	122	399	1.53e-13	72.8
MS.gene21888.t1	MTR_5g016350	23.410	346	191	11	83	426	201	474	2.15e-13	72.4
MS.gene21888.t1	MTR_5g016350	23.410	346	191	11	83	426	265	538	2.37e-13	72.4
MS.gene21888.t1	MTR_3g106080	24.194	310	192	9	155	434	326	622	1.60e-12	70.1
MS.gene21888.t1	MTR_3g106080	27.273	198	129	7	150	345	434	618	2.80e-12	69.3
MS.gene21888.t1	MTR_1g049000	40.426	94	46	3	200	284	8	100	1.48e-11	63.5
MS.gene21888.t1	MTR_5g022690	27.374	179	118	5	166	342	266	434	2.73e-11	65.9
MS.gene21888.t1	MTR_7g086360	22.857	210	150	4	220	427	53	252	7.15e-11	63.9
MS.gene21888.t1	MTR_1g072140	26.768	198	128	6	150	341	126	312	8.79e-11	63.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21888.t1	AT4G33270	79.778	450	89	2	7	454	8	457	0.0	759
MS.gene21888.t1	AT4G33260	80.317	442	85	2	15	454	6	447	0.0	737
MS.gene21888.t1	AT4G33260	80.535	411	78	2	15	423	6	416	0.0	701
MS.gene21888.t1	AT5G27570	69.623	451	130	5	7	454	4	450	0.0	642
MS.gene21888.t1	AT5G27080	69.585	434	126	4	23	454	13	442	0.0	623
MS.gene21888.t1	AT5G26900	68.578	436	132	3	20	454	13	444	0.0	612
MS.gene21888.t1	AT5G27945	62.634	372	123	5	95	454	61	428	1.40e-162	466
MS.gene21888.t1	AT4G22910	42.822	411	217	4	33	433	62	464	3.27e-115	347
MS.gene21888.t1	AT5G13840	51.974	304	138	3	126	429	163	458	1.13e-110	335
MS.gene21888.t1	AT5G13840	51.974	304	138	3	126	429	163	458	1.13e-110	335
MS.gene21888.t1	AT4G11920	41.556	450	227	9	5	433	22	456	2.28e-110	334
MS.gene21888.t1	AT1G11160	29.101	189	119	7	159	345	40	215	3.82e-16	81.6
MS.gene21888.t1	AT1G11160	29.101	189	119	7	159	345	40	215	4.21e-16	81.6
MS.gene21888.t1	AT1G11160	29.101	189	119	7	159	345	40	215	4.33e-16	81.6
MS.gene21888.t1	AT5G08390	26.891	238	155	9	140	371	19	243	9.43e-16	80.5
MS.gene21888.t1	AT1G61210	26.201	229	141	8	138	351	6	221	1.50e-15	80.1
MS.gene21888.t1	AT1G61210	26.201	229	141	8	138	351	6	221	1.70e-15	79.7
MS.gene21888.t1	AT1G61210	26.201	229	141	8	138	351	6	221	1.70e-15	79.7
MS.gene21888.t1	AT1G11160	29.341	167	105	6	181	345	18	173	1.96e-15	79.3
MS.gene21888.t1	AT5G23430	27.189	217	140	8	140	351	19	222	1.35e-14	76.6
MS.gene21888.t1	AT5G23430	27.189	217	140	8	140	351	19	222	1.40e-14	76.6
MS.gene21888.t1	AT5G23430	27.189	217	140	8	140	351	19	222	1.40e-14	76.6
MS.gene21888.t1	AT5G52820	27.451	255	152	12	182	428	244	473	7.93e-14	73.9
MS.gene21888.t1	AT5G25150	26.000	300	183	10	155	428	329	615	2.41e-13	72.8
MS.gene21888.t1	AT5G25150	27.551	196	127	7	150	343	433	615	1.96e-11	66.6
MS.gene21888.t1	AT5G25150	26.000	300	183	10	155	428	329	615	2.86e-13	72.4
MS.gene21888.t1	AT5G25150	27.551	196	127	7	150	343	433	615	2.17e-11	66.6
MS.gene21888.t1	AT3G49660	23.922	255	175	9	178	426	71	312	7.45e-13	69.7
MS.gene21888.t1	AT3G49660	25.373	201	125	9	150	341	128	312	9.88e-11	63.2
MS.gene21888.t1	AT5G56130	23.077	208	117	4	259	427	16	219	1.05e-11	66.2
MS.gene21888.t1	AT5G67320	24.221	289	166	12	143	427	330	569	1.54e-11	67.0
MS.gene21888.t1	AT2G41500	25.614	285	153	11	149	430	311	539	1.59e-11	66.6
MS.gene21888.t1	AT2G41500	25.614	285	153	11	149	430	311	539	1.59e-11	66.6
MS.gene21888.t1	AT5G16750	25.238	210	144	7	149	354	32	232	1.89e-11	67.0
MS.gene21888.t1	AT2G32700	24.453	274	180	13	159	426	532	784	4.40e-11	65.9
MS.gene21888.t1	AT2G32700	24.453	274	180	13	159	426	534	786	4.56e-11	65.5
MS.gene21888.t1	AT2G32700	24.453	274	180	13	159	426	534	786	4.56e-11	65.5
MS.gene21888.t1	AT2G32700	24.453	274	180	13	159	426	534	786	4.56e-11	65.5
MS.gene21888.t1	AT2G32700	24.453	274	180	13	159	426	534	786	4.56e-11	65.5
MS.gene21888.t1	AT2G32700	24.453	274	180	13	159	426	534	786	4.56e-11	65.5
MS.gene21888.t1	AT2G32700	24.453	274	180	13	159	426	553	805	5.16e-11	65.5
MS.gene21888.t1	AT5G13480	26.383	235	149	10	182	409	137	354	6.92e-11	65.1
MS.gene21888.t1	AT5G13480	26.383	235	149	10	182	409	114	331	8.15e-11	64.7
MS.gene21888.t1	AT5G13480	26.383	235	149	10	182	409	114	331	8.18e-11	64.7
MS.gene21888.t1	AT5G13480	26.383	235	149	10	182	409	126	343	9.99e-11	64.3

Find 128 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATAATTCGCCTGGGCTTTA+TGG	0.172694	5.1:-2215661	None:intergenic
CAGACGAAACATTGAGGTTT+TGG	0.210103	5.1:+2217012	MS.gene21888:CDS
CTGGAAGGGGAGCACACTTC+TGG	0.268112	5.1:-2215501	None:intergenic
CTCTCTCTAACAATGGCTTC+AGG	0.319079	5.1:+2214870	None:intergenic
ATGCGGCTCACATGTGAAAA+TGG	0.326418	5.1:-2217092	None:intergenic
GTTCTTGAAAGCTAGAATTC+TGG	0.331154	5.1:-2215571	None:intergenic
ATGATAATCTACTTTACATC+TGG	0.357040	5.1:+2216604	MS.gene21888:CDS
GCGATCGAACAATCAAGTTT+TGG	0.357857	5.1:+2216757	MS.gene21888:CDS
TAGCTTCCTGTACGCTTCTC+TGG	0.359002	5.1:-2215520	None:intergenic
AATGGCTCAGCTCTTGCTTT+CGG	0.359726	5.1:-2217074	None:intergenic
CCCTATCACATCTGTGAGTT+GGG	0.371621	5.1:+2216203	MS.gene21888:CDS
CACTTCAAGAGTTCTTTATA+TGG	0.372662	5.1:+2216950	MS.gene21888:CDS
TCCATAGCAGGGAACATACT+TGG	0.385318	5.1:-2216824	None:intergenic
GTTGCCACTGTGCAACCATC+CGG	0.387110	5.1:-2216981	None:intergenic
AAATCCATCGCCGATCTATT+CGG	0.388532	5.1:-2215429	None:intergenic
GCTCCTGATGGGCGTCATAT+TGG	0.389395	5.1:+2216225	MS.gene21888:CDS
CCTGAAGAGGAAAACGTGAT+TGG	0.389938	5.1:-2214920	None:intergenic
GGATACAGCTTCAGATAAAC+AGG	0.391975	5.1:+2216281	MS.gene21888:CDS
CAGAGTCTTTAAACATGAAC+AGG	0.397977	5.1:+2215547	MS.gene21888:CDS
GAACTTTGAAAGGTGGTCAT+AGG	0.402652	5.1:+2216400	MS.gene21888:CDS
ATTACCTCAATTTATTAGAC+TGG	0.406978	5.1:+2216076	MS.gene21888:CDS
CTGAAGAGGAAAACGTGATT+GGG	0.412005	5.1:-2214919	None:intergenic
TCTGCCATCTTCACCATTGA+AGG	0.425176	5.1:-2216915	None:intergenic
AAGAAGTTTGCGGATTGAAA+TGG	0.432752	5.1:+2216547	MS.gene21888:CDS
CCCAAGTATGTTCCCTGCTA+TGG	0.435862	5.1:+2216823	MS.gene21888:CDS
TAGGCAGCGAGTAGGGTCTT+TGG	0.438523	5.1:+2216419	MS.gene21888:CDS
TAATAGTCATCCACAAGGTC+TGG	0.439377	5.1:-2216057	None:intergenic
TTTCATTCTTGTTCCATAGC+AGG	0.442202	5.1:-2216836	None:intergenic
CACAGAATCAGCTAACACTT+TGG	0.446675	5.1:+2216886	MS.gene21888:CDS
GCCCAACTCACAGATGTGAT+AGG	0.447194	5.1:-2216204	None:intergenic
GCCCTATCACATCTGTGAGT+TGG	0.447852	5.1:+2216202	MS.gene21888:CDS
CAAGGGAATTTGCTAGCTAC+TGG	0.450097	5.1:+2216723	MS.gene21888:CDS
CTTGCTTGGTGTCCATTCCA+AGG	0.450852	5.1:+2216705	MS.gene21888:CDS
CTCTGTAAGTGTTCCTGAAG+AGG	0.453227	5.1:-2214933	None:intergenic
TGCTTGAACTCGGTTGACAC+TGG	0.455069	5.1:+2216798	MS.gene21888:CDS
TCATGAGGAATCAAATCAAC+TGG	0.456090	5.1:-2215606	None:intergenic
TTGGAAACACAGTGTATTTG+TGG	0.457657	5.1:+2216127	MS.gene21888:CDS
TCTTTATATGGCGCAGAGCC+CGG	0.458141	5.1:+2216962	MS.gene21888:CDS
CTCCTACACAGTGGCTTCAC+AGG	0.468541	5.1:+2216652	MS.gene21888:CDS
TCAGACTTCTGAGAGGACTT+TGG	0.470401	5.1:+2216029	MS.gene21888:intron
TCCTGTACGCTTCTCTGGAA+GGG	0.470590	5.1:-2215515	None:intergenic
GATGGGCGTCATATTGGCAT+TGG	0.470950	5.1:+2216231	MS.gene21888:CDS
CTAGCTACTGGTGGAGGCAG+TGG	0.480596	5.1:+2216735	MS.gene21888:CDS
TTCATTCTTGTTCCATAGCA+GGG	0.481145	5.1:-2216835	None:intergenic
ACAACTCTGAAGTGCAGCTA+TGG	0.484474	5.1:+2216259	MS.gene21888:CDS
ATGCCAATATGACGCCCATC+AGG	0.488601	5.1:-2216228	None:intergenic
ACTTTGGAAATATCCTTCAA+TGG	0.495344	5.1:+2216902	MS.gene21888:CDS
ATATCCTTCAATGGTGAAGA+TGG	0.498891	5.1:+2216911	MS.gene21888:CDS
AACAACCACATACTCACAAC+CGG	0.499451	5.1:+2216447	MS.gene21888:CDS
TGCTATGGAACAAGAATGAA+AGG	0.505330	5.1:+2216838	MS.gene21888:CDS
TTCCTGTACGCTTCTCTGGA+AGG	0.508481	5.1:-2215516	None:intergenic
TTGCCACTGTGCAACCATCC+GGG	0.511124	5.1:-2216980	None:intergenic
TTGCTTGGTGTCCATTCCAA+GGG	0.513094	5.1:+2216706	MS.gene21888:CDS
TCTGTGAGTTGGGCTCCTGA+TGG	0.513830	5.1:+2216213	MS.gene21888:CDS
CACCGGTGCATGCTTGAACT+CGG	0.514125	5.1:+2216788	MS.gene21888:CDS
TTTCGATTATGCACACTACA+TGG	0.515001	5.1:+2215449	MS.gene21888:CDS
CTGTGAGTTGGGCTCCTGAT+GGG	0.515660	5.1:+2216214	MS.gene21888:CDS
CCCCTTCCAGAGAAGCGTAC+AGG	0.515877	5.1:+2215514	MS.gene21888:CDS
AGATCATACATCTGCAGTAA+AGG	0.521203	5.1:+2216680	MS.gene21888:CDS
AGGGAGTTACTCAGCTCTCA+TGG	0.521335	5.1:+2216858	MS.gene21888:CDS
CACCTGAGGAATAATTCGCC+TGG	0.521725	5.1:-2215670	None:intergenic
CACACTACATGGTGACAGAA+GGG	0.522072	5.1:+2215460	MS.gene21888:CDS
GGGCATGAACAAGAAGTTTG+CGG	0.522362	5.1:+2216537	MS.gene21888:CDS
TAGATTATCATTCCCTCCAC+TGG	0.527433	5.1:-2216592	None:intergenic
CTGCAGTTGAGAACTTTGAA+AGG	0.529944	5.1:+2216390	MS.gene21888:intron
TTACCTCAATTTATTAGACT+GGG	0.529967	5.1:+2216077	MS.gene21888:CDS
AATGTTCTTGCAATAGCACT+TGG	0.530924	5.1:+2216108	MS.gene21888:CDS
CCTGTACGCTTCTCTGGAAG+GGG	0.531549	5.1:-2215514	None:intergenic
GCCCAGGCGAATTATTCCTC+AGG	0.535877	5.1:+2215668	MS.gene21888:CDS
GACAGAAGGGGCTAAAGGTA+AGG	0.539789	5.1:+2215473	MS.gene21888:CDS
ATGGTGACAGAAGGGGCTAA+AGG	0.542605	5.1:+2215468	MS.gene21888:CDS
GCTAGCAAATTCCCTTGGAA+TGG	0.543063	5.1:-2216717	None:intergenic
GCTTGAACTCGGTTGACACT+GGG	0.546774	5.1:+2216799	MS.gene21888:CDS
ACCTGAGGAATAATTCGCCT+GGG	0.547062	5.1:-2215669	None:intergenic
TTTGGACGCTCCAGACCTTG+TGG	0.549436	5.1:+2216047	MS.gene21888:CDS
CAACTCTGAAGTGCAGCTAT+GGG	0.552386	5.1:+2216260	MS.gene21888:CDS
CTGCAGTAAAGGCTCTTGCT+TGG	0.552397	5.1:+2216691	MS.gene21888:CDS
GTGGTCATAGGCAGCGAGTA+GGG	0.553407	5.1:+2216412	MS.gene21888:CDS
GGAATCAAATCAACTGGAGT+AGG	0.555155	5.1:-2215600	None:intergenic
GTTCAAGCATGCACCGGTGT+GGG	0.559486	5.1:-2216784	None:intergenic
GTGAAGATGGCAGAGCTCAA+TGG	0.559588	5.1:+2216924	MS.gene21888:CDS
TGATAATCTACTTTACATCT+GGG	0.563072	5.1:+2216605	MS.gene21888:CDS
CCCAACTCACAGATGTGATA+GGG	0.567907	5.1:-2216203	None:intergenic
GCACACTACATGGTGACAGA+AGG	0.570973	5.1:+2215459	MS.gene21888:CDS
CTACTTTACATCTGGGACAG+AGG	0.574017	5.1:+2216612	MS.gene21888:CDS
TTCCCCAGTCTAATAAATTG+AGG	0.574191	5.1:-2216080	None:intergenic
CATACTCACAACCGGAGGGA+TGG	0.577159	5.1:+2216455	MS.gene21888:CDS
GATGATGAAGTAAGCTCATG+AGG	0.578213	5.1:-2215621	None:intergenic
TCCCTCCGGTTGTGAGTATG+TGG	0.579028	5.1:-2216452	None:intergenic
TATATGGCGCAGAGCCCGGA+TGG	0.579495	5.1:+2216966	MS.gene21888:CDS
CAGATTTATACCGAATAGAT+CGG	0.585167	5.1:+2215419	MS.gene21888:CDS
TATTTGTGGGATGCATCAAA+TGG	0.585182	5.1:+2216141	MS.gene21888:CDS
CAGTAGCTAGCAAATTCCCT+TGG	0.590454	5.1:-2216722	None:intergenic
CTCACAACCGGAGGGATGGA+CGG	0.590810	5.1:+2216459	MS.gene21888:CDS
AACCGAGTTCAAGCATGCAC+CGG	0.591621	5.1:-2216790	None:intergenic
TGGAAACACAGTGTATTTGT+GGG	0.594599	5.1:+2216128	MS.gene21888:CDS
GGTGGTCATAGGCAGCGAGT+AGG	0.596714	5.1:+2216411	MS.gene21888:CDS
AATTTGCTAGCTACTGGTGG+AGG	0.597622	5.1:+2216729	MS.gene21888:CDS
CAGCAGCAGACGAAACATTG+AGG	0.598798	5.1:+2217006	MS.gene21888:CDS
GGGAATTTGCTAGCTACTGG+TGG	0.599431	5.1:+2216726	MS.gene21888:CDS
TATACCGAATAGATCGGCGA+TGG	0.599857	5.1:+2215425	MS.gene21888:CDS
ATCAAATCAACTGGAGTAGG+TGG	0.601482	5.1:-2215597	None:intergenic
AGCGAGTAGGGTCTTTGGCA+TGG	0.601597	5.1:+2216424	MS.gene21888:CDS
GGATTGAAATGGTCGGCTAA+CGG	0.603805	5.1:+2216558	MS.gene21888:CDS
AGTTCAAGCATGCACCGGTG+TGG	0.604168	5.1:-2216785	None:intergenic
ATGTTACTGCTTATCGAATG+CGG	0.611907	5.1:-2217109	None:intergenic
AAGATAAAACCATAAAGCCC+AGG	0.612129	5.1:+2215652	MS.gene21888:CDS
CCATAGCAGGGAACATACTT+GGG	0.613427	5.1:-2216823	None:intergenic
AGGGCCGTCTTCTTCTTCGA+CGG	0.614774	5.1:-2216184	None:intergenic
AAGTGCTATTGCAAGAACAT+TGG	0.617527	5.1:-2216106	None:intergenic
CAGTTGAGAACTTTGAAAGG+TGG	0.617886	5.1:+2216393	MS.gene21888:intron
GTCAACAATTAGCCAGTGGA+GGG	0.619435	5.1:+2216580	MS.gene21888:CDS
GGTCAACAATTAGCCAGTGG+AGG	0.633558	5.1:+2216579	MS.gene21888:CDS
TACCTCAATTTATTAGACTG+GGG	0.642053	5.1:+2216078	MS.gene21888:CDS
GCTATGGAACAAGAATGAAA+GGG	0.642631	5.1:+2216839	MS.gene21888:CDS
ACCACATACTCACAACCGGA+GGG	0.650856	5.1:+2216451	MS.gene21888:CDS
AGCCTGTGAAGCCACTGTGT+AGG	0.662766	5.1:-2216654	None:intergenic
ACACTACATGGTGACAGAAG+GGG	0.666148	5.1:+2215461	MS.gene21888:CDS
AACGGTCAACAATTAGCCAG+TGG	0.669539	5.1:+2216576	MS.gene21888:CDS
CTTACAGAGAAAGAGTTCCA+AGG	0.680127	5.1:+2214947	MS.gene21888:CDS
GTTACCGTCGAAGAAGAAGA+CGG	0.688864	5.1:+2216180	MS.gene21888:CDS
ACATTGTTGAAACATACAGA+GGG	0.688874	5.1:+2216517	MS.gene21888:CDS
AACCACATACTCACAACCGG+AGG	0.695066	5.1:+2216450	MS.gene21888:CDS
CACATTGTTGAAACATACAG+AGG	0.702329	5.1:+2216516	MS.gene21888:CDS
AGTTTGCGGATTGAAATGGT+CGG	0.710202	5.1:+2216551	MS.gene21888:CDS
TGAGGTAATAGTCATCCACA+AGG	0.712406	5.1:-2216062	None:intergenic
CTTCTAGTTCTCCTACACAG+TGG	0.755247	5.1:+2216643	MS.gene21888:CDS
GAGCCCGGATGGTTGCACAG+TGG	0.763260	5.1:+2216977	MS.gene21888:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTATTAATTCTTAGTTAAAA+GGG	+	chr5.1:2215814-2215833	MS.gene21888:intron	10.0%
!!	TTTATTAATTCTTAGTTAAA+AGG	+	chr5.1:2215813-2215832	MS.gene21888:intron	10.0%
!!!	AAGAATAAATTTTGAAATTT+TGG	+	chr5.1:2215097-2215116	MS.gene21888:intron	10.0%
!!!	AGAATAAATTTTGAAATTTT+GGG	+	chr5.1:2215098-2215117	MS.gene21888:intron	10.0%
!!!	TGTGAATAAATTTTAAAATT+GGG	+	chr5.1:2215134-2215153	MS.gene21888:intron	10.0%
!!!	TTGTGAATAAATTTTAAAAT+TGG	+	chr5.1:2215133-2215152	MS.gene21888:intron	10.0%
!!	AATAACAATGATAAAACATA+AGG	-	chr5.1:2215720-2215739	None:intergenic	15.0%
!!	ACTCATTCAAAATTTAAATT+TGG	-	chr5.1:2215766-2215785	None:intergenic	15.0%
!!!	AATAAATTTTGAAATTTTGG+GGG	+	chr5.1:2215100-2215119	MS.gene21888:intron	15.0%
!!!	ATGTTTTATCATTGTTATTT+TGG	+	chr5.1:2215721-2215740	MS.gene21888:intron	15.0%
!!!	GAATAAATTTTGAAATTTTG+GGG	+	chr5.1:2215099-2215118	MS.gene21888:intron	15.0%
!!!	GGATTTTAATTATCAAATTT+TGG	+	chr5.1:2215032-2215051	MS.gene21888:intron	15.0%
!!	ATTCAGATCTTTCAAAATTA+GGG	+	chr5.1:2214994-2215013	MS.gene21888:intron	20.0%
!!	CACAAAATTGCAAAATATTA+TGG	-	chr5.1:2215986-2216005	None:intergenic	20.0%
!!	TATTCAGATCTTTCAAAATT+AGG	+	chr5.1:2214993-2215012	MS.gene21888:intron	20.0%
!!!	ATTAGGGTTTTAATGTAAAT+TGG	+	chr5.1:2215010-2215029	MS.gene21888:intron	20.0%
!!!	ATTATCAAATTTTGGTGTTT+TGG	+	chr5.1:2215040-2215059	MS.gene21888:intron	20.0%
!!!	ATTTTTAATTTTCGAATTGG+GGG	+	chr5.1:2215157-2215176	MS.gene21888:intron	20.0%
!!!	GATTTTTAATTTTCGAATTG+GGG	+	chr5.1:2215156-2215175	MS.gene21888:intron	20.0%
!!!	GGATTTTTAATTTTCGAATT+GGG	+	chr5.1:2215155-2215174	MS.gene21888:intron	20.0%
!!!	TTAGGGTTTTAATGTAAATT+GGG	+	chr5.1:2215011-2215030	MS.gene21888:intron	20.0%
!	AAACCAAACAAGAATAATGT+TGG	-	chr5.1:2216370-2216389	None:intergenic	25.0%
!	ATGATAATCTACTTTACATC+TGG	+	chr5.1:2216604-2216623	MS.gene21888:CDS	25.0%
!	ATTACCTCAATTTATTAGAC+TGG	+	chr5.1:2216076-2216095	MS.gene21888:CDS	25.0%
!	TGATAATCTACTTTACATCT+GGG	+	chr5.1:2216605-2216624	MS.gene21888:CDS	25.0%
!	TTACCTCAATTTATTAGACT+GGG	+	chr5.1:2216077-2216096	MS.gene21888:CDS	25.0%
!	TTAGACTACTAATCCAATTA+TGG	-	chr5.1:2215066-2215085	None:intergenic	25.0%
!!	AAATGTTCTTACATTTTCCT+TGG	-	chr5.1:2214967-2214986	None:intergenic	25.0%
!!	AGGAATTTGATAGTTCATAA+AGG	-	chr5.1:2216338-2216357	None:intergenic	25.0%
!!!	AATGAGTTTTGAAAATCAAG+TGG	+	chr5.1:2215779-2215798	MS.gene21888:intron	25.0%
!!!	ATGAGTTTTGAAAATCAAGT+GGG	+	chr5.1:2215780-2215799	MS.gene21888:intron	25.0%
!!!	GGGATTTTTAATTTTCGAAT+TGG	+	chr5.1:2215154-2215173	MS.gene21888:intron	25.0%
!!!	TCATTGTTATTTTGGTTCTT+TGG	+	chr5.1:2215729-2215748	MS.gene21888:intron	25.0%
!!!	TTAGGTTTTTGAAATTCAAG+TGG	+	chr5.1:2215904-2215923	MS.gene21888:intron	25.0%
	AAGGTGTATTAATGTTAACC+AGG	-	chr5.1:2216319-2216338	None:intergenic	30.0%
	ACATTGTTGAAACATACAGA+GGG	+	chr5.1:2216517-2216536	MS.gene21888:CDS	30.0%
	CACTTCAAGAGTTCTTTATA+TGG	+	chr5.1:2216950-2216969	MS.gene21888:CDS	30.0%
	CAGATTTATACCGAATAGAT+CGG	+	chr5.1:2215419-2215438	MS.gene21888:CDS	30.0%
	CTTAGTTAAAAGGGTTTGTA+TGG	+	chr5.1:2215823-2215842	MS.gene21888:intron	30.0%
	TACCTCAATTTATTAGACTG+GGG	+	chr5.1:2216078-2216097	MS.gene21888:CDS	30.0%
	TTGCCAACATTATTCTTGTT+TGG	+	chr5.1:2216364-2216383	MS.gene21888:intron	30.0%
!	ACTTTGGAAATATCCTTCAA+TGG	+	chr5.1:2216902-2216921	MS.gene21888:CDS	30.0%
!!	TCTTAGTTGAAAGTGTTGTA+TGG	+	chr5.1:2215947-2215966	MS.gene21888:intron	30.0%
	AAGATAAAACCATAAAGCCC+AGG	+	chr5.1:2215652-2215671	MS.gene21888:CDS	35.0%
	AATAATGTTGGCAAACTAGC+AGG	-	chr5.1:2216358-2216377	None:intergenic	35.0%
	AATGTTCTTGCAATAGCACT+TGG	+	chr5.1:2216108-2216127	MS.gene21888:CDS	35.0%
	AGATCATACATCTGCAGTAA+AGG	+	chr5.1:2216680-2216699	MS.gene21888:CDS	35.0%
	ATATCCTTCAATGGTGAAGA+TGG	+	chr5.1:2216911-2216930	MS.gene21888:CDS	35.0%
	CACATTGTTGAAACATACAG+AGG	+	chr5.1:2216516-2216535	MS.gene21888:CDS	35.0%
	CAGAGTCTTTAAACATGAAC+AGG	+	chr5.1:2215547-2215566	MS.gene21888:CDS	35.0%
	GCTATGGAACAAGAATGAAA+GGG	+	chr5.1:2216839-2216858	MS.gene21888:CDS	35.0%
	GTTCTTGAAAGCTAGAATTC+TGG	-	chr5.1:2215574-2215593	None:intergenic	35.0%
	TATTTGTGGGATGCATCAAA+TGG	+	chr5.1:2216141-2216160	MS.gene21888:CDS	35.0%
	TCATGAGGAATCAAATCAAC+TGG	-	chr5.1:2215609-2215628	None:intergenic	35.0%
	TGCTATGGAACAAGAATGAA+AGG	+	chr5.1:2216838-2216857	MS.gene21888:CDS	35.0%
	TGGAAACACAGTGTATTTGT+GGG	+	chr5.1:2216128-2216147	MS.gene21888:CDS	35.0%
	TTCATTCTTGTTCCATAGCA+GGG	-	chr5.1:2216838-2216857	None:intergenic	35.0%
	TTCCCCAGTCTAATAAATTG+AGG	-	chr5.1:2216083-2216102	None:intergenic	35.0%
	TTGGAAACACAGTGTATTTG+TGG	+	chr5.1:2216127-2216146	MS.gene21888:CDS	35.0%
	TTTCATTCTTGTTCCATAGC+AGG	-	chr5.1:2216839-2216858	None:intergenic	35.0%
	TTTCGATTATGCACACTACA+TGG	+	chr5.1:2215449-2215468	MS.gene21888:CDS	35.0%
!	AACAGGTTTTTACACTTACC+TGG	+	chr5.1:2216298-2216317	MS.gene21888:intron	35.0%
!	AAGAAGTTTGCGGATTGAAA+TGG	+	chr5.1:2216547-2216566	MS.gene21888:CDS	35.0%
!	AAGTGCTATTGCAAGAACAT+TGG	-	chr5.1:2216109-2216128	None:intergenic	35.0%
!	GATTAGTTGGCTATCTGATT+AGG	+	chr5.1:2215886-2215905	MS.gene21888:intron	35.0%
!!	AAAACGTGATTGGGTTTTGA+TGG	-	chr5.1:2214913-2214932	None:intergenic	35.0%
!!	TGTGCTAGTTGATGATTAGT+TGG	+	chr5.1:2215873-2215892	MS.gene21888:intron	35.0%
!!	TTGATTTGTTTGTTGCCAGT+TGG	+	chr5.1:2215395-2215414	MS.gene21888:intron	35.0%
!!	TTGTTTTTCAGACTTCTGAG+AGG	+	chr5.1:2216022-2216041	MS.gene21888:intron	35.0%
!!!	TTGAGGTTTTGGAATGCTTT+TGG	+	chr5.1:2217023-2217042	MS.gene21888:CDS	35.0%
!!!	TTTGGTGTTTTGGCCATAAT+TGG	+	chr5.1:2215050-2215069	MS.gene21888:intron	35.0%
	AAATCCATCGCCGATCTATT+CGG	-	chr5.1:2215432-2215451	None:intergenic	40.0%
	AACAACCACATACTCACAAC+CGG	+	chr5.1:2216447-2216466	MS.gene21888:CDS	40.0%
	AATAATTCGCCTGGGCTTTA+TGG	-	chr5.1:2215664-2215683	None:intergenic	40.0%
	AGATTTCAGTAACACACCTG+AGG	-	chr5.1:2215687-2215706	None:intergenic	40.0%
	ATCAAATCAACTGGAGTAGG+TGG	-	chr5.1:2215600-2215619	None:intergenic	40.0%
	CACAGAATCAGCTAACACTT+TGG	+	chr5.1:2216886-2216905	MS.gene21888:CDS	40.0%
	CTGAAGAGGAAAACGTGATT+GGG	-	chr5.1:2214922-2214941	None:intergenic	40.0%
	CTGCAGTTGAGAACTTTGAA+AGG	+	chr5.1:2216390-2216409	MS.gene21888:intron	40.0%
	CTTACAGAGAAAGAGTTCCA+AGG	+	chr5.1:2214947-2214966	MS.gene21888:CDS	40.0%
	GATGATGAAGTAAGCTCATG+AGG	-	chr5.1:2215624-2215643	None:intergenic	40.0%
	GGAATCAAATCAACTGGAGT+AGG	-	chr5.1:2215603-2215622	None:intergenic	40.0%
	GGATACAGCTTCAGATAAAC+AGG	+	chr5.1:2216281-2216300	MS.gene21888:CDS	40.0%
	TAATAGTCATCCACAAGGTC+TGG	-	chr5.1:2216060-2216079	None:intergenic	40.0%
	TAGATTATCATTCCCTCCAC+TGG	-	chr5.1:2216595-2216614	None:intergenic	40.0%
	TCGGTATAAATCTGTCCAAC+TGG	-	chr5.1:2215413-2215432	None:intergenic	40.0%
	TGAGGTAATAGTCATCCACA+AGG	-	chr5.1:2216065-2216084	None:intergenic	40.0%
!	AGTTTGCGGATTGAAATGGT+CGG	+	chr5.1:2216551-2216570	MS.gene21888:CDS	40.0%
!	CAGACGAAACATTGAGGTTT+TGG	+	chr5.1:2217012-2217031	MS.gene21888:CDS	40.0%
!	CAGTTGAGAACTTTGAAAGG+TGG	+	chr5.1:2216393-2216412	MS.gene21888:intron	40.0%
!	GAACTTTGAAAGGTGGTCAT+AGG	+	chr5.1:2216400-2216419	MS.gene21888:CDS	40.0%
!	GCGATCGAACAATCAAGTTT+TGG	+	chr5.1:2216757-2216776	MS.gene21888:CDS	40.0%
!	TTGGGGGTTTTCTTAGATTG+TGG	+	chr5.1:2215116-2215135	MS.gene21888:intron	40.0%
!!!	AAGTTTTGGAATACCCACAC+CGG	+	chr5.1:2216771-2216790	MS.gene21888:CDS	40.0%
	AACGGTCAACAATTAGCCAG+TGG	+	chr5.1:2216576-2216595	MS.gene21888:CDS	45.0%
	AATGGCTCAGCTCTTGCTTT+CGG	-	chr5.1:2217077-2217096	None:intergenic	45.0%
	ACACTACATGGTGACAGAAG+GGG	+	chr5.1:2215461-2215480	MS.gene21888:CDS	45.0%
	ACCTGAGGAATAATTCGCCT+GGG	-	chr5.1:2215672-2215691	None:intergenic	45.0%
	ATGCGGCTCACATGTGAAAA+TGG	-	chr5.1:2217095-2217114	None:intergenic	45.0%
	CACACTACATGGTGACAGAA+GGG	+	chr5.1:2215460-2215479	MS.gene21888:CDS	45.0%
	CAGTAGCTAGCAAATTCCCT+TGG	-	chr5.1:2216725-2216744	None:intergenic	45.0%
	CCATAGCAGGGAACATACTT+GGG	-	chr5.1:2216826-2216845	None:intergenic	45.0%
	CCCAACTCACAGATGTGATA+GGG	-	chr5.1:2216206-2216225	None:intergenic	45.0%
	CCCTATCACATCTGTGAGTT+GGG	+	chr5.1:2216203-2216222	MS.gene21888:CDS	45.0%
	CCTGAAGAGGAAAACGTGAT+TGG	-	chr5.1:2214923-2214942	None:intergenic	45.0%
	CTCTGTAAGTGTTCCTGAAG+AGG	-	chr5.1:2214936-2214955	None:intergenic	45.0%
	GCTAGCAAATTCCCTTGGAA+TGG	-	chr5.1:2216720-2216739	None:intergenic	45.0%
	GGATTGAAATGGTCGGCTAA+CGG	+	chr5.1:2216558-2216577	MS.gene21888:CDS	45.0%
	GGGCATGAACAAGAAGTTTG+CGG	+	chr5.1:2216537-2216556	MS.gene21888:CDS	45.0%
	GTCAACAATTAGCCAGTGGA+GGG	+	chr5.1:2216580-2216599	MS.gene21888:CDS	45.0%
	GTTACCGTCGAAGAAGAAGA+CGG	+	chr5.1:2216180-2216199	MS.gene21888:CDS	45.0%
	TATACCGAATAGATCGGCGA+TGG	+	chr5.1:2215425-2215444	MS.gene21888:CDS	45.0%
	TCCATAGCAGGGAACATACT+TGG	-	chr5.1:2216827-2216846	None:intergenic	45.0%
	TCTGCCATCTTCACCATTGA+AGG	-	chr5.1:2216918-2216937	None:intergenic	45.0%
!	AATTTTACCGTCCATCCCTC+CGG	-	chr5.1:2216469-2216488	None:intergenic	45.0%
!	CCAATCACGTTTTCCTCTTC+AGG	+	chr5.1:2214920-2214939	MS.gene21888:CDS	45.0%
!	CTACTTTACATCTGGGACAG+AGG	+	chr5.1:2216612-2216631	MS.gene21888:CDS	45.0%
!	GCAGCTTTTGCTACTACTTC+TGG	-	chr5.1:2217053-2217072	None:intergenic	45.0%
!	TCAGACTTCTGAGAGGACTT+TGG	+	chr5.1:2216029-2216048	MS.gene21888:intron	45.0%
!!	AATTTGCTAGCTACTGGTGG+AGG	+	chr5.1:2216729-2216748	MS.gene21888:CDS	45.0%
!!	ACAACTCTGAAGTGCAGCTA+TGG	+	chr5.1:2216259-2216278	MS.gene21888:CDS	45.0%
!!	ACGTGATTGGGTTTTGATGG+CGG	-	chr5.1:2214910-2214929	None:intergenic	45.0%
!!	CAACTCTGAAGTGCAGCTAT+GGG	+	chr5.1:2216260-2216279	MS.gene21888:CDS	45.0%
!!	CAAGGGAATTTGCTAGCTAC+TGG	+	chr5.1:2216723-2216742	MS.gene21888:CDS	45.0%
!!	CTTCTAGTTCTCCTACACAG+TGG	+	chr5.1:2216643-2216662	MS.gene21888:CDS	45.0%
!!	TTGCTTGGTGTCCATTCCAA+GGG	+	chr5.1:2216706-2216725	MS.gene21888:CDS	45.0%
	AACCACATACTCACAACCGG+AGG	+	chr5.1:2216450-2216469	MS.gene21888:CDS	50.0%
	AACCGAGTTCAAGCATGCAC+CGG	-	chr5.1:2216793-2216812	None:intergenic	50.0%
	ACCACATACTCACAACCGGA+GGG	+	chr5.1:2216451-2216470	MS.gene21888:CDS	50.0%
	AGGGAGTTACTCAGCTCTCA+TGG	+	chr5.1:2216858-2216877	MS.gene21888:CDS	50.0%
	ATGCCAATATGACGCCCATC+AGG	-	chr5.1:2216231-2216250	None:intergenic	50.0%
	ATGGTGACAGAAGGGGCTAA+AGG	+	chr5.1:2215468-2215487	MS.gene21888:CDS	50.0%
	CACCTGAGGAATAATTCGCC+TGG	-	chr5.1:2215673-2215692	None:intergenic	50.0%
	CAGCAGCAGACGAAACATTG+AGG	+	chr5.1:2217006-2217025	MS.gene21888:CDS	50.0%
	CCCAAGTATGTTCCCTGCTA+TGG	+	chr5.1:2216823-2216842	MS.gene21888:CDS	50.0%
	CTGCAGTAAAGGCTCTTGCT+TGG	+	chr5.1:2216691-2216710	MS.gene21888:CDS	50.0%
	GACAGAAGGGGCTAAAGGTA+AGG	+	chr5.1:2215473-2215492	MS.gene21888:CDS	50.0%
	GATGGGCGTCATATTGGCAT+TGG	+	chr5.1:2216231-2216250	MS.gene21888:CDS	50.0%
	GCACACTACATGGTGACAGA+AGG	+	chr5.1:2215459-2215478	MS.gene21888:CDS	50.0%
	GCCCAACTCACAGATGTGAT+AGG	-	chr5.1:2216207-2216226	None:intergenic	50.0%
	GCCCTATCACATCTGTGAGT+TGG	+	chr5.1:2216202-2216221	MS.gene21888:CDS	50.0%
	GCTTGAACTCGGTTGACACT+GGG	+	chr5.1:2216799-2216818	MS.gene21888:CDS	50.0%
	GGTCAACAATTAGCCAGTGG+AGG	+	chr5.1:2216579-2216598	MS.gene21888:CDS	50.0%
	GTGAAGATGGCAGAGCTCAA+TGG	+	chr5.1:2216924-2216943	MS.gene21888:CDS	50.0%
	TAGCTTCCTGTACGCTTCTC+TGG	-	chr5.1:2215523-2215542	None:intergenic	50.0%
	TCTTTATATGGCGCAGAGCC+CGG	+	chr5.1:2216962-2216981	MS.gene21888:CDS	50.0%
	TGCTTGAACTCGGTTGACAC+TGG	+	chr5.1:2216798-2216817	MS.gene21888:CDS	50.0%
	TTCCTGTACGCTTCTCTGGA+AGG	-	chr5.1:2215519-2215538	None:intergenic	50.0%
!	TCCTGTACGCTTCTCTGGAA+GGG	-	chr5.1:2215518-2215537	None:intergenic	50.0%
!!	CGTGATTGGGTTTTGATGGC+GGG	-	chr5.1:2214909-2214928	None:intergenic	50.0%
!!	CTTGCTTGGTGTCCATTCCA+AGG	+	chr5.1:2216705-2216724	MS.gene21888:CDS	50.0%
!!	GGGAATTTGCTAGCTACTGG+TGG	+	chr5.1:2216726-2216745	MS.gene21888:CDS	50.0%
	AGCCTGTGAAGCCACTGTGT+AGG	-	chr5.1:2216657-2216676	None:intergenic	55.0%
	AGCGAGTAGGGTCTTTGGCA+TGG	+	chr5.1:2216424-2216443	MS.gene21888:CDS	55.0%
	AGGGCCGTCTTCTTCTTCGA+CGG	-	chr5.1:2216187-2216206	None:intergenic	55.0%
	CACCGGTGCATGCTTGAACT+CGG	+	chr5.1:2216788-2216807	MS.gene21888:CDS	55.0%
	CATACTCACAACCGGAGGGA+TGG	+	chr5.1:2216455-2216474	MS.gene21888:CDS	55.0%
	CTCCTACACAGTGGCTTCAC+AGG	+	chr5.1:2216652-2216671	MS.gene21888:CDS	55.0%
	GCCCAGGCGAATTATTCCTC+AGG	+	chr5.1:2215668-2215687	MS.gene21888:CDS	55.0%
	GTGGTCATAGGCAGCGAGTA+GGG	+	chr5.1:2216412-2216431	MS.gene21888:CDS	55.0%
	GTTGCCACTGTGCAACCATC+CGG	-	chr5.1:2216984-2217003	None:intergenic	55.0%
	TAGGCAGCGAGTAGGGTCTT+TGG	+	chr5.1:2216419-2216438	MS.gene21888:CDS	55.0%
	TCCCTCCGGTTGTGAGTATG+TGG	-	chr5.1:2216455-2216474	None:intergenic	55.0%
	TTGCCACTGTGCAACCATCC+GGG	-	chr5.1:2216983-2217002	None:intergenic	55.0%
	TTTGGACGCTCCAGACCTTG+TGG	+	chr5.1:2216047-2216066	MS.gene21888:CDS	55.0%
!	AGTTCAAGCATGCACCGGTG+TGG	-	chr5.1:2216788-2216807	None:intergenic	55.0%
!	CCTGTACGCTTCTCTGGAAG+GGG	-	chr5.1:2215517-2215536	None:intergenic	55.0%
!	CTGTGAGTTGGGCTCCTGAT+GGG	+	chr5.1:2216214-2216233	MS.gene21888:CDS	55.0%
!	GCTCCTGATGGGCGTCATAT+TGG	+	chr5.1:2216225-2216244	MS.gene21888:CDS	55.0%
!	GTTCAAGCATGCACCGGTGT+GGG	-	chr5.1:2216787-2216806	None:intergenic	55.0%
!	TCTGTGAGTTGGGCTCCTGA+TGG	+	chr5.1:2216213-2216232	MS.gene21888:CDS	55.0%
	CTAGCTACTGGTGGAGGCAG+TGG	+	chr5.1:2216735-2216754	MS.gene21888:CDS	60.0%
	CTCACAACCGGAGGGATGGA+CGG	+	chr5.1:2216459-2216478	MS.gene21888:CDS	60.0%
	GGTGGTCATAGGCAGCGAGT+AGG	+	chr5.1:2216411-2216430	MS.gene21888:CDS	60.0%
	TATATGGCGCAGAGCCCGGA+TGG	+	chr5.1:2216966-2216985	MS.gene21888:CDS	60.0%
!	CCCCTTCCAGAGAAGCGTAC+AGG	+	chr5.1:2215514-2215533	MS.gene21888:CDS	60.0%
!	CTGGAAGGGGAGCACACTTC+TGG	-	chr5.1:2215504-2215523	None:intergenic	60.0%
	GAGCCCGGATGGTTGCACAG+TGG	+	chr5.1:2216977-2216996	MS.gene21888:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	2214882	2217121	2214882	ID=MS.gene21888
chr5.1	mRNA	2214882	2217121	2214882	ID=MS.gene21888.t1;Parent=MS.gene21888
chr5.1	exon	2214882	2214974	2214882	ID=MS.gene21888.t1.exon1;Parent=MS.gene21888.t1
chr5.1	CDS	2214882	2214974	2214882	ID=cds.MS.gene21888.t1;Parent=MS.gene21888.t1
chr5.1	exon	2215414	2215689	2215414	ID=MS.gene21888.t1.exon2;Parent=MS.gene21888.t1
chr5.1	CDS	2215414	2215689	2215414	ID=cds.MS.gene21888.t1;Parent=MS.gene21888.t1
chr5.1	exon	2216033	2216302	2216033	ID=MS.gene21888.t1.exon3;Parent=MS.gene21888.t1
chr5.1	CDS	2216033	2216302	2216033	ID=cds.MS.gene21888.t1;Parent=MS.gene21888.t1
chr5.1	exon	2216396	2217121	2216396	ID=MS.gene21888.t1.exon4;Parent=MS.gene21888.t1
chr5.1	CDS	2216396	2217121	2216396	ID=cds.MS.gene21888.t1;Parent=MS.gene21888.t1

Gene Sequence

>MS.gene21888

ATGGCTTCAGGTTCTTTGAGTTCTCCCGCCATCAAAACCCAATCACGTTTTCCTCTTCAGGAACACTTACAGAGAAAGAGTTCCAAGGAAAATGTAAGAACATTTTTCAATTATTCAGATCTTTCAAAATTAGGGTTTTAATGTAAATTGGGATTTTAATTATCAAATTTTGGTGTTTTGGCCATAATTGGATTAGTAGTCTAAAAACTGTGATAAAGAATAAATTTTGAAATTTTGGGGGTTTTCTTAGATTGTGAATAAATTTTAAAATTGGGATTTTTAATTTTCGAATTGGGGGTTTTCTTAGATTGTGGATTAATTTTGAAAATATGTTAAAAAAGAATGAATTTTGACATTATGTCATGAAAAAGAATGAATTTTGACATTATGTCGTGAAAAAGAATGAATTTTGAAATTATGCTAACTTTTTATTGACTGAGTTGAAATTATGATATGATAAGTTGATAATATTCTGAATTTGTTTATGAAAATCAAGTATTGATTCTTTATTGTTTGATTTGTTTGTTGCCAGTTGGACAGATTTATACCGAATAGATCGGCGATGGATTTCGATTATGCACACTACATGGTGACAGAAGGGGCTAAAGGTAAGGAAAATCCAGAAGTGTGCTCCCCTTCCAGAGAAGCGTACAGGAAGCTACTTGCAGAGTCTTTAAACATGAACAGGACCAGAATTCTAGCTTTCAAGAACAAGCCACCTACTCCAGTTGATTTGATTCCTCATGAGCTTACTTCATCATCTCTTCAACAAGATAAAACCATAAAGCCCAGGCGAATTATTCCTCAGGTGTGTTACTGAAATCTGATTGATATTCCTTATGTTTTATCATTGTTATTTTGGTTCTTTGGTTGCATGAACTCCAAATTTAAATTTTGAATGAGTTTTGAAAATCAAGTGGGTCTGAGTTGTTTTATTAATTCTTAGTTAAAAGGGTTTGTATGGTGAATGAAATTCAATATTTTGCAATTTTGTGCTAGTTGATGATTAGTTGGCTATCTGATTAGGTTTTTGAAATTCAAGTGGATCTGAGTTGTTTTCTTAATTCTTAGTTGAAAGTGTTGTATGGTATAAACTGTAAACCATAATATTTTGCAATTTTGTGCTAATTGATTGATATGTTGTTTTTCAGACTTCTGAGAGGACTTTGGACGCTCCAGACCTTGTGGATGACTATTACCTCAATTTATTAGACTGGGGAAGCGCCAATGTTCTTGCAATAGCACTTGGAAACACAGTGTATTTGTGGGATGCATCAAATGGTTCTACTTCAGAACTTGTTACCGTCGAAGAAGAAGACGGCCCTATCACATCTGTGAGTTGGGCTCCTGATGGGCGTCATATTGGCATTGGTTTGAACAACTCTGAAGTGCAGCTATGGGATACAGCTTCAGATAAACAGGTTTTTACACTTACCTGGTTAACATTAATACACCTTTATGAACTATCAAATTCCTGCTAGTTTGCCAACATTATTCTTGTTTGGTTTCTGCAGTTGAGAACTTTGAAAGGTGGTCATAGGCAGCGAGTAGGGTCTTTGGCATGGAACAACCACATACTCACAACCGGAGGGATGGACGGTAAAATTATCAATAATGATGTAAGAATTAGAGCACACATTGTTGAAACATACAGAGGGCATGAACAAGAAGTTTGCGGATTGAAATGGTCGGCTAACGGTCAACAATTAGCCAGTGGAGGGAATGATAATCTACTTTACATCTGGGACAGAGGTACATCAACTTCTAGTTCTCCTACACAGTGGCTTCACAGGCTTGAAGATCATACATCTGCAGTAAAGGCTCTTGCTTGGTGTCCATTCCAAGGGAATTTGCTAGCTACTGGTGGAGGCAGTGGCGATCGAACAATCAAGTTTTGGAATACCCACACCGGTGCATGCTTGAACTCGGTTGACACTGGGTCCCAAGTATGTTCCCTGCTATGGAACAAGAATGAAAGGGAGTTACTCAGCTCTCATGGTTTTACACAGAATCAGCTAACACTTTGGAAATATCCTTCAATGGTGAAGATGGCAGAGCTCAATGGTCACACTTCAAGAGTTCTTTATATGGCGCAGAGCCCGGATGGTTGCACAGTGGCAACAGCAGCAGCAGACGAAACATTGAGGTTTTGGAATGCTTTTGGCACTCCAGAAGTAGTAGCAAAAGCTGCACCGAAAGCAAGAGCTGAGCCATTTTCACATGTGAGCCGCATTCGATAA

Protein sequence

>MS.gene21888.t1

MASGSLSSPAIKTQSRFPLQEHLQRKSSKENLDRFIPNRSAMDFDYAHYMVTEGAKGKENPEVCSPSREAYRKLLAESLNMNRTRILAFKNKPPTPVDLIPHELTSSSLQQDKTIKPRRIIPQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWDASNGSTSELVTVEEEDGPITSVSWAPDGRHIGIGLNNSEVQLWDTASDKQLRTLKGGHRQRVGSLAWNNHILTTGGMDGKIINNDVRIRAHIVETYRGHEQEVCGLKWSANGQQLASGGNDNLLYIWDRGTSTSSSPTQWLHRLEDHTSAVKALAWCPFQGNLLATGGGSGDRTIKFWNTHTGACLNSVDTGSQVCSLLWNKNERELLSSHGFTQNQLTLWKYPSMVKMAELNGHTSRVLYMAQSPDGCTVATAAADETLRFWNAFGTPEVVAKAAPKARAEPFSHVSRIR