AlfalfaGEDB | MS.gene21734

M. sativa cultivar XinJiangDaYe / MS.gene21734

KEGG: K03363
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00400.35 PF12894.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21734.t1	XP_003610876.1	99.1	454	4	0	1	454	1	454	1.50E-265	924.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21734.t1	Q9SZA4	79.8	450	89	2	7	454	8	457	2.4e-216	752.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21734.t1	G7K6J6	99.1	454	4	0	1	454	1	454	1.1e-265	924.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049298	MS.gene21734	0.854688	1.04E-61	-1.69E-46
MS.gene050707	MS.gene21734	0.813	3.19E-51	-1.69E-46
MS.gene050875	MS.gene21734	0.802124	6.51E-49	-1.69E-46
MS.gene051816	MS.gene21734	0.802952	4.39E-49	-1.69E-46
MS.gene051896	MS.gene21734	0.819816	9.52E-53	-1.69E-46
MS.gene051914	MS.gene21734	0.871136	8.60E-67	-1.69E-46
MS.gene051955	MS.gene21734	0.813669	2.28E-51	-1.69E-46
MS.gene052209	MS.gene21734	0.812354	4.42E-51	-1.69E-46
MS.gene052308	MS.gene21734	0.909107	8.21E-82	-1.69E-46
MS.gene052367	MS.gene21734	0.821611	3.68E-53	-1.69E-46
MS.gene053173	MS.gene21734	0.856254	3.64E-62	-1.69E-46
MS.gene053348	MS.gene21734	0.865815	4.49E-65	-1.69E-46
MS.gene054551	MS.gene21734	0.883761	3.41E-71	-1.69E-46
MS.gene054552	MS.gene21734	0.820706	5.95E-53	-1.69E-46
MS.gene054593	MS.gene21734	0.887124	1.88E-72	-1.69E-46
MS.gene054633	MS.gene21734	0.850275	1.89E-60	-1.69E-46
MS.gene054662	MS.gene21734	0.833698	4.59E-56	-1.69E-46
MS.gene054850	MS.gene21734	0.826888	2.12E-54	-1.69E-46
MS.gene054885	MS.gene21734	0.818072	2.37E-52	-1.69E-46
MS.gene054941	MS.gene21734	0.861034	1.36E-63	-1.69E-46
MS.gene055308	MS.gene21734	0.89589	6.23E-76	-1.69E-46
MS.gene055578	MS.gene21734	0.874163	8.37E-68	-1.69E-46
MS.gene055579	MS.gene21734	0.813491	2.49E-51	-1.69E-46
MS.gene055957	MS.gene21734	0.846456	2.15E-59	-1.69E-46
MS.gene056041	MS.gene21734	0.881986	1.52E-70	-1.69E-46
MS.gene056042	MS.gene21734	0.819453	1.15E-52	-1.69E-46
MS.gene05640	MS.gene21734	0.896991	2.17E-76	-1.69E-46
MS.gene056526	MS.gene21734	0.802745	4.85E-49	-1.69E-46
MS.gene056528	MS.gene21734	0.859182	4.94E-63	-1.69E-46
MS.gene05652	MS.gene21734	0.88575	6.21E-72	-1.69E-46
MS.gene056618	MS.gene21734	0.821499	3.91E-53	-1.69E-46
MS.gene057101	MS.gene21734	0.838948	2.12E-57	-1.69E-46
MS.gene057367	MS.gene21734	0.850562	1.57E-60	-1.69E-46
MS.gene057458	MS.gene21734	0.844361	7.93E-59	-1.69E-46
MS.gene057669	MS.gene21734	0.842832	2.03E-58	-1.69E-46
MS.gene058013	MS.gene21734	0.829656	4.56E-55	-1.69E-46
MS.gene058128	MS.gene21734	0.965515	1.05E-124	-1.69E-46
MS.gene058853	MS.gene21734	0.923462	2.57E-89	-1.69E-46
MS.gene05926	MS.gene21734	0.808533	2.96E-50	-1.69E-46
MS.gene059553	MS.gene21734	0.854271	1.38E-61	-1.69E-46
MS.gene059616	MS.gene21734	0.892709	1.24E-74	-1.69E-46
MS.gene059905	MS.gene21734	0.834638	2.67E-56	-1.69E-46
MS.gene060473	MS.gene21734	0.830871	2.30E-55	-1.69E-46
MS.gene060565	MS.gene21734	0.809773	1.60E-50	-1.69E-46
MS.gene06093	MS.gene21734	0.8656	5.25E-65	-1.69E-46
MS.gene061099	MS.gene21734	0.864381	1.27E-64	-1.69E-46
MS.gene061260	MS.gene21734	0.823961	1.04E-53	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene21734	MS.gene008833	PPI
MS.gene71204	MS.gene21734	PPI
MS.gene064035	MS.gene21734	PPI
MS.gene77885	MS.gene21734	PPI
MS.gene21734	MS.gene82740	PPI
MS.gene36842	MS.gene21734	PPI
MS.gene21734	MS.gene48655	PPI
MS.gene99554	MS.gene21734	PPI
MS.gene21734	MS.gene066046	PPI
MS.gene058128	MS.gene21734	PPI
MS.gene41291	MS.gene21734	PPI
MS.gene011448	MS.gene21734	PPI
MS.gene066501	MS.gene21734	PPI
MS.gene66342	MS.gene21734	PPI
MS.gene63085	MS.gene21734	PPI
MS.gene21734	MS.gene027073	PPI
MS.gene21734	MS.gene027072	PPI
MS.gene056455	MS.gene21734	PPI
MS.gene21734	MS.gene65463	PPI
MS.gene21734	MS.gene29768	PPI
MS.gene030294	MS.gene21734	PPI
MS.gene89986	MS.gene21734	PPI
MS.gene064762	MS.gene21734	PPI
MS.gene35613	MS.gene21734	PPI
MS.gene21734	MS.gene067705	PPI
MS.gene21734	MS.gene025229	PPI
MS.gene69965	MS.gene21734	PPI
MS.gene21734	MS.gene37226	PPI
MS.gene21734	MS.gene049021	PPI
MS.gene21734	MS.gene043742	PPI
MS.gene21734	MS.gene027075	PPI
MS.gene21734	MS.gene027441	PPI
MS.gene21734	MS.gene022502	PPI
MS.gene21734	MS.gene050457	PPI
MS.gene82020	MS.gene21734	PPI
MS.gene21734	MS.gene66343	PPI
MS.gene21734	MS.gene99973	PPI
MS.gene21734	MS.gene30174	PPI
MS.gene21734	MS.gene26021	PPI
MS.gene30172	MS.gene21734	PPI
MS.gene21734	MS.gene064435	PPI
MS.gene23852	MS.gene21734	PPI
MS.gene97943	MS.gene21734	PPI
MS.gene21734	MS.gene91775	PPI
MS.gene67157	MS.gene21734	PPI
MS.gene21734	MS.gene25515	PPI
MS.gene90626	MS.gene21734	PPI
MS.gene69396	MS.gene21734	PPI
MS.gene012203	MS.gene21734	PPI
MS.gene21734	MS.gene81663	PPI
MS.gene25244	MS.gene21734	PPI
MS.gene21734	MS.gene050270	PPI
MS.gene21734	MS.gene34712	PPI
MS.gene063222	MS.gene21734	PPI
MS.gene060495	MS.gene21734	PPI
MS.gene069995	MS.gene21734	PPI
MS.gene21734	MS.gene81660	PPI
MS.gene21734	MS.gene037184	PPI
MS.gene21734	MS.gene004844	PPI
MS.gene049022	MS.gene21734	PPI
MS.gene69687	MS.gene21734	PPI
MS.gene21734	MS.gene99588	PPI
MS.gene21734	MS.gene038519	PPI
MS.gene21734	MS.gene27223	PPI
MS.gene21734	MS.gene045133	PPI
MS.gene21734	MS.gene042783	PPI
MS.gene23810	MS.gene21734	PPI
MS.gene21734	MS.gene20160	PPI
MS.gene78281	MS.gene21734	PPI
MS.gene21734	MS.gene067322	PPI
MS.gene21734	MS.gene050272	PPI
MS.gene21734	MS.gene050456	PPI
MS.gene21734	MS.gene51258	PPI
MS.gene060496	MS.gene21734	PPI
MS.gene21734	MS.gene32723	PPI
MS.gene69966	MS.gene21734	PPI
MS.gene21734	MS.gene067321	PPI
MS.gene24806	MS.gene21734	PPI
MS.gene071113	MS.gene21734	PPI
MS.gene52355	MS.gene21734	PPI
MS.gene21734	MS.gene29842	PPI
MS.gene21734	MS.gene053363	PPI
MS.gene027071	MS.gene21734	PPI
MS.gene21734	MS.gene024753	PPI
MS.gene21734	MS.gene34651	PPI
MS.gene21734	MS.gene75493	PPI
MS.gene21734	MS.gene32726	PPI
MS.gene21734	MS.gene71122	PPI
MS.gene21734	MS.gene55882	PPI
MS.gene010910	MS.gene21734	PPI
MS.gene066464	MS.gene21734	PPI
MS.gene21734	MS.gene052956	PPI
MS.gene07706	MS.gene21734	PPI
MS.gene027074	MS.gene21734	PPI
MS.gene21734	MS.gene51262	PPI
MS.gene21734	MS.gene75059	PPI
MS.gene21734	MS.gene24951	PPI
MS.gene21734	MS.gene26672	PPI
MS.gene21734	MS.gene92674	PPI
MS.gene21734	MS.gene014931	PPI
MS.gene21734	MS.gene21888	PPI
MS.gene21734	MS.gene97379	PPI
MS.gene010912	MS.gene21734	PPI
MS.gene21734	MS.gene066500	PPI
MS.gene21734	MS.gene91851	PPI
MS.gene23811	MS.gene21734	PPI
MS.gene46611	MS.gene21734	PPI
MS.gene51335	MS.gene21734	PPI
MS.gene82021	MS.gene21734	PPI
MS.gene81658	MS.gene21734	PPI
MS.gene84465	MS.gene21734	PPI
MS.gene21734	MS.gene014419	PPI
MS.gene21734	MS.gene26019	PPI
MS.gene21734	MS.gene022109	PPI
MS.gene037950	MS.gene21734	PPI
MS.gene21734	MS.gene98540	PPI
MS.gene21734	MS.gene62819	PPI
MS.gene068249	MS.gene21734	PPI
MS.gene21734	MS.gene30176	PPI
MS.gene21734	MS.gene65775	PPI
MS.gene64441	MS.gene21734	PPI
MS.gene21734	MS.gene80163	PPI
MS.gene21734	MS.gene42253	PPI
MS.gene21734	MS.gene55883	PPI
MS.gene21734	MS.gene033401	PPI
MS.gene21734	MS.gene33377	PPI
MS.gene21734	MS.gene045937	PPI
MS.gene21734	MS.gene068273	PPI
MS.gene21734	MS.gene06177	PPI
MS.gene21734	MS.gene51278	PPI
MS.gene21734	MS.gene052188	PPI
MS.gene21734	MS.gene87218	PPI
MS.gene21734	MS.gene85534	PPI
MS.gene20161	MS.gene21734	PPI
MS.gene039682	MS.gene21734	PPI
MS.gene21734	MS.gene014321	PPI
MS.gene21734	MS.gene064862	PPI
MS.gene21734	MS.gene23881	PPI
MS.gene21734	MS.gene06176	PPI
MS.gene24922	MS.gene21734	PPI
MS.gene33406	MS.gene21734	PPI
MS.gene21734	MS.gene062662	PPI
MS.gene066467	MS.gene21734	PPI
MS.gene21734	MS.gene30177	PPI
MS.gene21734	MS.gene84466	PPI
MS.gene21734	MS.gene70008	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21734.t1	MTR_5g008010	99.119	454	4	0	1	454	1	454	0.0	937
MS.gene21734.t1	MTR_3g051690	91.503	459	34	4	1	454	1	459	0.0	872
MS.gene21734.t1	MTR_7g106870	75.223	448	105	5	12	454	11	457	0.0	707
MS.gene21734.t1	MTR_7g451830	66.966	445	126	7	15	454	3	431	0.0	591
MS.gene21734.t1	MTR_7g047640	66.966	445	126	7	15	454	3	431	0.0	591
MS.gene21734.t1	MTR_7g057540	65.011	443	135	7	14	454	2	426	0.0	563
MS.gene21734.t1	MTR_7g055590	64.932	442	135	7	14	453	2	425	0.0	561
MS.gene21734.t1	MTR_7g053100	61.607	448	147	8	17	454	4	436	0.0	537
MS.gene21734.t1	MTR_7g034625	71.160	319	90	1	124	440	13	331	1.14e-172	488
MS.gene21734.t1	MTR_2g100540	51.945	437	160	13	25	454	4	397	2.69e-135	395
MS.gene21734.t1	MTR_2g100460	56.322	348	133	6	107	452	52	382	2.03e-129	380
MS.gene21734.t1	MTR_2g060710	45.388	412	202	8	33	430	26	428	9.05e-123	365
MS.gene21734.t1	MTR_3g067940	45.012	411	201	7	33	429	57	456	2.79e-115	347
MS.gene21734.t1	MTR_4g102510	41.611	447	218	9	11	433	29	456	4.74e-113	341
MS.gene21734.t1	MTR_2g012630	27.386	241	156	9	140	374	18	245	1.42e-17	86.3
MS.gene21734.t1	MTR_2g012630	27.386	241	156	9	140	374	18	245	1.61e-17	85.9
MS.gene21734.t1	MTR_4g415070	61.111	54	21	0	379	432	57	110	6.98e-16	79.0
MS.gene21734.t1	MTR_6g004040	26.891	238	155	9	140	371	19	243	3.61e-15	78.6
MS.gene21734.t1	MTR_5g016370	23.630	292	162	8	143	426	312	550	8.55e-15	77.0
MS.gene21734.t1	MTR_4g095058	26.336	262	168	11	177	427	287	534	2.81e-14	75.5
MS.gene21734.t1	MTR_4g095058	26.336	262	168	11	177	427	285	532	3.23e-14	75.1
MS.gene21734.t1	MTR_1g054300	27.778	252	152	11	182	426	253	481	3.68e-14	74.7
MS.gene21734.t1	MTR_1g054300	25.510	294	156	10	182	428	122	399	1.53e-13	72.8
MS.gene21734.t1	MTR_5g016350	23.410	346	191	11	83	426	201	474	2.15e-13	72.4
MS.gene21734.t1	MTR_5g016350	23.410	346	191	11	83	426	265	538	2.37e-13	72.4
MS.gene21734.t1	MTR_3g106080	24.194	310	192	9	155	434	326	622	1.60e-12	70.1
MS.gene21734.t1	MTR_3g106080	27.273	198	129	7	150	345	434	618	2.80e-12	69.3
MS.gene21734.t1	MTR_1g049000	40.426	94	46	3	200	284	8	100	1.48e-11	63.5
MS.gene21734.t1	MTR_5g022690	27.374	179	118	5	166	342	266	434	2.73e-11	65.9
MS.gene21734.t1	MTR_7g086360	22.857	210	150	4	220	427	53	252	7.15e-11	63.9
MS.gene21734.t1	MTR_1g072140	26.768	198	128	6	150	341	126	312	8.79e-11	63.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21734.t1	AT4G33270	79.778	450	89	2	7	454	8	457	0.0	759
MS.gene21734.t1	AT4G33260	80.317	442	85	2	15	454	6	447	0.0	737
MS.gene21734.t1	AT4G33260	80.535	411	78	2	15	423	6	416	0.0	701
MS.gene21734.t1	AT5G27570	69.623	451	130	5	7	454	4	450	0.0	642
MS.gene21734.t1	AT5G27080	69.585	434	126	4	23	454	13	442	0.0	623
MS.gene21734.t1	AT5G26900	68.578	436	132	3	20	454	13	444	0.0	612
MS.gene21734.t1	AT5G27945	62.634	372	123	5	95	454	61	428	1.40e-162	466
MS.gene21734.t1	AT4G22910	42.822	411	217	4	33	433	62	464	3.27e-115	347
MS.gene21734.t1	AT5G13840	51.974	304	138	3	126	429	163	458	1.13e-110	335
MS.gene21734.t1	AT5G13840	51.974	304	138	3	126	429	163	458	1.13e-110	335
MS.gene21734.t1	AT4G11920	41.556	450	227	9	5	433	22	456	2.28e-110	334
MS.gene21734.t1	AT1G11160	29.101	189	119	7	159	345	40	215	3.82e-16	81.6
MS.gene21734.t1	AT1G11160	29.101	189	119	7	159	345	40	215	4.21e-16	81.6
MS.gene21734.t1	AT1G11160	29.101	189	119	7	159	345	40	215	4.33e-16	81.6
MS.gene21734.t1	AT5G08390	26.891	238	155	9	140	371	19	243	9.43e-16	80.5
MS.gene21734.t1	AT1G61210	26.201	229	141	8	138	351	6	221	1.50e-15	80.1
MS.gene21734.t1	AT1G61210	26.201	229	141	8	138	351	6	221	1.70e-15	79.7
MS.gene21734.t1	AT1G61210	26.201	229	141	8	138	351	6	221	1.70e-15	79.7
MS.gene21734.t1	AT1G11160	29.341	167	105	6	181	345	18	173	1.96e-15	79.3
MS.gene21734.t1	AT5G23430	27.189	217	140	8	140	351	19	222	1.35e-14	76.6
MS.gene21734.t1	AT5G23430	27.189	217	140	8	140	351	19	222	1.40e-14	76.6
MS.gene21734.t1	AT5G23430	27.189	217	140	8	140	351	19	222	1.40e-14	76.6
MS.gene21734.t1	AT5G52820	27.451	255	152	12	182	428	244	473	7.93e-14	73.9
MS.gene21734.t1	AT5G25150	26.000	300	183	10	155	428	329	615	2.41e-13	72.8
MS.gene21734.t1	AT5G25150	27.551	196	127	7	150	343	433	615	1.96e-11	66.6
MS.gene21734.t1	AT5G25150	26.000	300	183	10	155	428	329	615	2.86e-13	72.4
MS.gene21734.t1	AT5G25150	27.551	196	127	7	150	343	433	615	2.17e-11	66.6
MS.gene21734.t1	AT3G49660	23.922	255	175	9	178	426	71	312	7.45e-13	69.7
MS.gene21734.t1	AT3G49660	25.373	201	125	9	150	341	128	312	9.88e-11	63.2
MS.gene21734.t1	AT5G56130	23.077	208	117	4	259	427	16	219	1.05e-11	66.2
MS.gene21734.t1	AT5G67320	24.221	289	166	12	143	427	330	569	1.54e-11	67.0
MS.gene21734.t1	AT2G41500	25.614	285	153	11	149	430	311	539	1.59e-11	66.6
MS.gene21734.t1	AT2G41500	25.614	285	153	11	149	430	311	539	1.59e-11	66.6
MS.gene21734.t1	AT5G16750	25.238	210	144	7	149	354	32	232	1.89e-11	67.0
MS.gene21734.t1	AT2G32700	24.453	274	180	13	159	426	532	784	4.40e-11	65.9
MS.gene21734.t1	AT2G32700	24.453	274	180	13	159	426	534	786	4.56e-11	65.5
MS.gene21734.t1	AT2G32700	24.453	274	180	13	159	426	534	786	4.56e-11	65.5
MS.gene21734.t1	AT2G32700	24.453	274	180	13	159	426	534	786	4.56e-11	65.5
MS.gene21734.t1	AT2G32700	24.453	274	180	13	159	426	534	786	4.56e-11	65.5
MS.gene21734.t1	AT2G32700	24.453	274	180	13	159	426	534	786	4.56e-11	65.5
MS.gene21734.t1	AT2G32700	24.453	274	180	13	159	426	553	805	5.16e-11	65.5
MS.gene21734.t1	AT5G13480	26.383	235	149	10	182	409	137	354	6.92e-11	65.1
MS.gene21734.t1	AT5G13480	26.383	235	149	10	182	409	114	331	8.15e-11	64.7
MS.gene21734.t1	AT5G13480	26.383	235	149	10	182	409	114	331	8.18e-11	64.7
MS.gene21734.t1	AT5G13480	26.383	235	149	10	182	409	126	343	9.99e-11	64.3

Find 128 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATAATTCGCCTGGGCTTTA+TGG	0.172694	5.2:-2189899	None:intergenic
CAGACGAAACATTGAGGTTT+TGG	0.210103	5.2:+2191250	MS.gene21734:CDS
CTGGAAGGGGAGCACACTTC+TGG	0.268112	5.2:-2189739	None:intergenic
CTCTCTCTAACAATGGCTTC+AGG	0.319079	5.2:+2189107	None:intergenic
ATGCGGCTCACATGTGAAAA+TGG	0.326418	5.2:-2191330	None:intergenic
GTTCTTGAAAGCTAGAATTC+TGG	0.331154	5.2:-2189809	None:intergenic
ATGATAATCTACTTTACATC+TGG	0.357450	5.2:+2190842	MS.gene21734:CDS
GCGATCGAACAATCAAGTTT+TGG	0.357857	5.2:+2190995	MS.gene21734:CDS
TAGCTTCCTGTACGCTTCTC+TGG	0.359002	5.2:-2189758	None:intergenic
AATGGCTCAGCTCTTGCTTT+CGG	0.359726	5.2:-2191312	None:intergenic
CCCTATCACATCTGTGAGTT+GGG	0.371621	5.2:+2190441	MS.gene21734:CDS
CACTTCAAGAGTTCTTTATA+TGG	0.372662	5.2:+2191188	MS.gene21734:CDS
TCCATAGCAGGGAACATACT+TGG	0.385318	5.2:-2191062	None:intergenic
GTTGCCACTGTGCAACCATC+CGG	0.387110	5.2:-2191219	None:intergenic
AAATCCATCGCCGATCTATT+CGG	0.388532	5.2:-2189667	None:intergenic
GCTCCTGATGGGCGTCATAT+TGG	0.389395	5.2:+2190463	MS.gene21734:CDS
CCTGAAGAGGAAAACGTGAT+TGG	0.389938	5.2:-2189157	None:intergenic
GGATACAGCTTCAGATAAAC+AGG	0.391975	5.2:+2190519	MS.gene21734:CDS
CAGAGTCTTTAAACATGAAC+AGG	0.397977	5.2:+2189785	MS.gene21734:CDS
GAACTTTGAAAGGTGGTCAT+AGG	0.402652	5.2:+2190638	MS.gene21734:CDS
ATTACCTCAATTTATTAGAC+TGG	0.406978	5.2:+2190314	MS.gene21734:CDS
CTGAAGAGGAAAACGTGATT+GGG	0.412005	5.2:-2189156	None:intergenic
TCTGCCATCTTCACCATTGA+AGG	0.425176	5.2:-2191153	None:intergenic
AAGAAGTTTGCGGATTGAAA+TGG	0.432752	5.2:+2190785	MS.gene21734:CDS
CCCAAGTATGTTCCCTGCTA+TGG	0.435862	5.2:+2191061	MS.gene21734:CDS
TAGGCAGCGAGTAGGGTCTT+TGG	0.438523	5.2:+2190657	MS.gene21734:CDS
TAATAGTCATCCACAAGGTC+TGG	0.439377	5.2:-2190295	None:intergenic
TTTCATTCTTGTTCCATAGC+AGG	0.442202	5.2:-2191074	None:intergenic
CACAGAATCAGCTAACACTT+TGG	0.446675	5.2:+2191124	MS.gene21734:CDS
GCCCAACTCACAGATGTGAT+AGG	0.447194	5.2:-2190442	None:intergenic
GCCCTATCACATCTGTGAGT+TGG	0.447852	5.2:+2190440	MS.gene21734:CDS
CAAGGGAATTTGCTAGCTAC+TGG	0.450097	5.2:+2190961	MS.gene21734:CDS
CTTGCTTGGTGTCCATTCCA+AGG	0.450852	5.2:+2190943	MS.gene21734:CDS
CTCTGTAAGTGTTCCTGAAG+AGG	0.453227	5.2:-2189170	None:intergenic
TGCTTGAACTCGGTTGACAC+TGG	0.455069	5.2:+2191036	MS.gene21734:CDS
TCATGAGGAATCAAATCAAC+TGG	0.456090	5.2:-2189844	None:intergenic
TTGGAAACACAGTGTATTTG+TGG	0.457657	5.2:+2190365	MS.gene21734:CDS
TCTTTATATGGCGCAGAGCC+CGG	0.458141	5.2:+2191200	MS.gene21734:CDS
CTCCTACACAGTGGCTTCAC+AGG	0.468541	5.2:+2190890	MS.gene21734:CDS
TCAGACTTCTGAGAGGACTT+TGG	0.470401	5.2:+2190267	MS.gene21734:intron
TCCTGTACGCTTCTCTGGAA+GGG	0.470590	5.2:-2189753	None:intergenic
GATGGGCGTCATATTGGCAT+TGG	0.470950	5.2:+2190469	MS.gene21734:CDS
CTAGCTACTGGTGGAGGCAG+TGG	0.480596	5.2:+2190973	MS.gene21734:CDS
TTCATTCTTGTTCCATAGCA+GGG	0.481145	5.2:-2191073	None:intergenic
ACAACTCTGAAGTGCAGCTA+TGG	0.484474	5.2:+2190497	MS.gene21734:CDS
ATGCCAATATGACGCCCATC+AGG	0.488601	5.2:-2190466	None:intergenic
ACTTTGGAAATATCCTTCAA+TGG	0.495344	5.2:+2191140	MS.gene21734:CDS
ATATCCTTCAATGGTGAAGA+TGG	0.498891	5.2:+2191149	MS.gene21734:CDS
AACAACCACATACTCACAAC+CGG	0.499451	5.2:+2190685	MS.gene21734:CDS
TGCTATGGAACAAGAATGAA+AGG	0.505330	5.2:+2191076	MS.gene21734:CDS
TTCCTGTACGCTTCTCTGGA+AGG	0.508481	5.2:-2189754	None:intergenic
GAGCCTTTACTGCAGATGTA+TGG	0.509251	5.2:-2190921	None:intergenic
TTGCCACTGTGCAACCATCC+GGG	0.511124	5.2:-2191218	None:intergenic
AGACCATACATCTGCAGTAA+AGG	0.511492	5.2:+2190918	MS.gene21734:CDS
TAGATTATCATTACCTCCAC+TGG	0.511866	5.2:-2190830	None:intergenic
TTGCTTGGTGTCCATTCCAA+GGG	0.513094	5.2:+2190944	MS.gene21734:CDS
TCTGTGAGTTGGGCTCCTGA+TGG	0.513830	5.2:+2190451	MS.gene21734:CDS
CACCGGTGCATGCTTGAACT+CGG	0.514125	5.2:+2191026	MS.gene21734:CDS
TTTCGATTATGCACACTACA+TGG	0.515001	5.2:+2189687	MS.gene21734:CDS
CTGTGAGTTGGGCTCCTGAT+GGG	0.515660	5.2:+2190452	MS.gene21734:CDS
CCCCTTCCAGAGAAGCGTAC+AGG	0.515877	5.2:+2189752	MS.gene21734:CDS
AGGGAGTTACTCAGCTCTCA+TGG	0.521335	5.2:+2191096	MS.gene21734:CDS
CACCTGAGGAATAATTCGCC+TGG	0.521725	5.2:-2189908	None:intergenic
CACACTACATGGTGACAGAA+GGG	0.522072	5.2:+2189698	MS.gene21734:CDS
GGGCATGAACAAGAAGTTTG+CGG	0.522362	5.2:+2190775	MS.gene21734:CDS
CTGCAGTTGAGAACTTTGAA+AGG	0.529944	5.2:+2190628	MS.gene21734:intron
TTACCTCAATTTATTAGACT+GGG	0.529967	5.2:+2190315	MS.gene21734:CDS
AATGTTCTTGCAATAGCACT+TGG	0.530924	5.2:+2190346	MS.gene21734:CDS
CCTGTACGCTTCTCTGGAAG+GGG	0.531549	5.2:-2189752	None:intergenic
GCCCAGGCGAATTATTCCTC+AGG	0.535877	5.2:+2189906	MS.gene21734:CDS
GACAGAAGGGGCTAAAGGTA+AGG	0.539789	5.2:+2189711	MS.gene21734:CDS
ATGGTGACAGAAGGGGCTAA+AGG	0.542605	5.2:+2189706	MS.gene21734:CDS
GCTAGCAAATTCCCTTGGAA+TGG	0.543063	5.2:-2190955	None:intergenic
GCTTGAACTCGGTTGACACT+GGG	0.546774	5.2:+2191037	MS.gene21734:CDS
ACCTGAGGAATAATTCGCCT+GGG	0.547062	5.2:-2189907	None:intergenic
TTTGGACGCTCCAGACCTTG+TGG	0.549436	5.2:+2190285	MS.gene21734:CDS
CAACTCTGAAGTGCAGCTAT+GGG	0.552386	5.2:+2190498	MS.gene21734:CDS
CTGCAGTAAAGGCTCTTGCT+TGG	0.552397	5.2:+2190929	MS.gene21734:CDS
GTGGTCATAGGCAGCGAGTA+GGG	0.553407	5.2:+2190650	MS.gene21734:CDS
GGAATCAAATCAACTGGAGT+AGG	0.555155	5.2:-2189838	None:intergenic
GTTCAAGCATGCACCGGTGT+GGG	0.559486	5.2:-2191022	None:intergenic
GTGAAGATGGCAGAGCTCAA+TGG	0.559588	5.2:+2191162	MS.gene21734:CDS
TGATAATCTACTTTACATCT+GGG	0.563482	5.2:+2190843	MS.gene21734:CDS
CCCAACTCACAGATGTGATA+GGG	0.567907	5.2:-2190441	None:intergenic
GCACACTACATGGTGACAGA+AGG	0.570973	5.2:+2189697	MS.gene21734:CDS
CTACTTTACATCTGGGACAG+AGG	0.574017	5.2:+2190850	MS.gene21734:CDS
TTCCCCAGTCTAATAAATTG+AGG	0.574191	5.2:-2190318	None:intergenic
CATACTCACAACCGGAGGGA+TGG	0.577159	5.2:+2190693	MS.gene21734:CDS
GATGATGAAGTAAGCTCATG+AGG	0.578213	5.2:-2189859	None:intergenic
TCCCTCCGGTTGTGAGTATG+TGG	0.579028	5.2:-2190690	None:intergenic
TATATGGCGCAGAGCCCGGA+TGG	0.579495	5.2:+2191204	MS.gene21734:CDS
CAGATTTATACCGAATAGAT+CGG	0.585167	5.2:+2189657	MS.gene21734:CDS
TATTTGTGGGATGCATCAAA+TGG	0.585182	5.2:+2190379	MS.gene21734:CDS
CAGTAGCTAGCAAATTCCCT+TGG	0.590454	5.2:-2190960	None:intergenic
CTCACAACCGGAGGGATGGA+CGG	0.590810	5.2:+2190697	MS.gene21734:CDS
AACCGAGTTCAAGCATGCAC+CGG	0.591621	5.2:-2191028	None:intergenic
TGGAAACACAGTGTATTTGT+GGG	0.594599	5.2:+2190366	MS.gene21734:CDS
GGTGGTCATAGGCAGCGAGT+AGG	0.596714	5.2:+2190649	MS.gene21734:CDS
AATTTGCTAGCTACTGGTGG+AGG	0.597622	5.2:+2190967	MS.gene21734:CDS
CAGCAGCAGACGAAACATTG+AGG	0.598798	5.2:+2191244	MS.gene21734:CDS
GGGAATTTGCTAGCTACTGG+TGG	0.599431	5.2:+2190964	MS.gene21734:CDS
TATACCGAATAGATCGGCGA+TGG	0.599857	5.2:+2189663	MS.gene21734:CDS
ATCAAATCAACTGGAGTAGG+TGG	0.601482	5.2:-2189835	None:intergenic
AGCGAGTAGGGTCTTTGGCA+TGG	0.601597	5.2:+2190662	MS.gene21734:CDS
GGATTGAAATGGTCGGCTAA+CGG	0.603805	5.2:+2190796	MS.gene21734:CDS
AGTTCAAGCATGCACCGGTG+TGG	0.604168	5.2:-2191023	None:intergenic
ATGTTACTGCTTATCGAATG+CGG	0.611907	5.2:-2191347	None:intergenic
AAGATAAAACCATAAAGCCC+AGG	0.612129	5.2:+2189890	MS.gene21734:CDS
CCATAGCAGGGAACATACTT+GGG	0.613427	5.2:-2191061	None:intergenic
AGGGCCGTCTTCTTCTTCGA+CGG	0.614774	5.2:-2190422	None:intergenic
AAGTGCTATTGCAAGAACAT+TGG	0.617527	5.2:-2190344	None:intergenic
CAGTTGAGAACTTTGAAAGG+TGG	0.617886	5.2:+2190631	MS.gene21734:intron
TACCTCAATTTATTAGACTG+GGG	0.642053	5.2:+2190316	MS.gene21734:CDS
GCTATGGAACAAGAATGAAA+GGG	0.642631	5.2:+2191077	MS.gene21734:CDS
ACCACATACTCACAACCGGA+GGG	0.650856	5.2:+2190689	MS.gene21734:CDS
AGCCTGTGAAGCCACTGTGT+AGG	0.662766	5.2:-2190892	None:intergenic
ACACTACATGGTGACAGAAG+GGG	0.666148	5.2:+2189699	MS.gene21734:CDS
AACGGTCAACAATTAGCCAG+TGG	0.669539	5.2:+2190814	MS.gene21734:CDS
CTTACAGAGAAAGAGTTCCA+AGG	0.680127	5.2:+2189184	MS.gene21734:CDS
GTTACCGTCGAAGAAGAAGA+CGG	0.688864	5.2:+2190418	MS.gene21734:CDS
ACATTGTTGAAACATACAGA+GGG	0.688874	5.2:+2190755	MS.gene21734:CDS
AACCACATACTCACAACCGG+AGG	0.695066	5.2:+2190688	MS.gene21734:CDS
GGTCAACAATTAGCCAGTGG+AGG	0.696916	5.2:+2190817	MS.gene21734:CDS
CACATTGTTGAAACATACAG+AGG	0.702329	5.2:+2190754	MS.gene21734:CDS
AGTTTGCGGATTGAAATGGT+CGG	0.710202	5.2:+2190789	MS.gene21734:CDS
TGAGGTAATAGTCATCCACA+AGG	0.712406	5.2:-2190300	None:intergenic
CTTCTAGTTCTCCTACACAG+TGG	0.755247	5.2:+2190881	MS.gene21734:CDS
GAGCCCGGATGGTTGCACAG+TGG	0.763260	5.2:+2191215	MS.gene21734:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTATTAATTCTTAGTTAAAA+GGG	+	chr5.2:2190052-2190071	MS.gene21734:intron	10.0%
!!	TTTATTAATTCTTAGTTAAA+AGG	+	chr5.2:2190051-2190070	MS.gene21734:intron	10.0%
!!!	AAGAATAAATTTTGAAATTT+TGG	+	chr5.2:2189334-2189353	MS.gene21734:intron	10.0%
!!!	AGAATAAATTTTGAAATTTT+GGG	+	chr5.2:2189335-2189354	MS.gene21734:intron	10.0%
!!!	TGTGAATAAATTTTAAAATT+GGG	+	chr5.2:2189371-2189390	MS.gene21734:intron	10.0%
!!!	TTGTGAATAAATTTTAAAAT+TGG	+	chr5.2:2189370-2189389	MS.gene21734:intron	10.0%
!!	AATAACAATGATAAAACATA+AGG	-	chr5.2:2189958-2189977	None:intergenic	15.0%
!!	ACTCATTCAAAATTTAAATT+TGG	-	chr5.2:2190004-2190023	None:intergenic	15.0%
!!!	AATAAATTTTGAAATTTTGG+GGG	+	chr5.2:2189337-2189356	MS.gene21734:intron	15.0%
!!!	ATGTTTTATCATTGTTATTT+TGG	+	chr5.2:2189959-2189978	MS.gene21734:intron	15.0%
!!!	ATTATCAAATTTTAGTGTTT+TGG	+	chr5.2:2189277-2189296	MS.gene21734:intron	15.0%
!!!	GAATAAATTTTGAAATTTTG+GGG	+	chr5.2:2189336-2189355	MS.gene21734:intron	15.0%
!!	ATTCAGATCTTTCAAAATTA+GGG	+	chr5.2:2189231-2189250	MS.gene21734:intron	20.0%
!!	CACAAAATTGCAAAATATTA+TGG	-	chr5.2:2190224-2190243	None:intergenic	20.0%
!!	TATTCAGATCTTTCAAAATT+AGG	+	chr5.2:2189230-2189249	MS.gene21734:intron	20.0%
!!!	ATTAGGGTTTTAATGTAAAT+TGG	+	chr5.2:2189247-2189266	MS.gene21734:intron	20.0%
!!!	ATTTTTAATTTTCGAATTGG+GGG	+	chr5.2:2189394-2189413	MS.gene21734:intron	20.0%
!!!	GATTTTTAATTTTCGAATTG+GGG	+	chr5.2:2189393-2189412	MS.gene21734:intron	20.0%
!!!	GGATTTTTAATTTTCGAATT+GGG	+	chr5.2:2189392-2189411	MS.gene21734:intron	20.0%
!!!	TTAGGGTTTTAATGTAAATT+GGG	+	chr5.2:2189248-2189267	MS.gene21734:intron	20.0%
!!!	TTAGGTTTTTGAAATTCAAA+TGG	+	chr5.2:2190142-2190161	MS.gene21734:intron	20.0%
!	AAACCAAACAAGAATAATGT+TGG	-	chr5.2:2190608-2190627	None:intergenic	25.0%
!	ATGATAATCTACTTTACATC+TGG	+	chr5.2:2190842-2190861	MS.gene21734:CDS	25.0%
!	ATTACCTCAATTTATTAGAC+TGG	+	chr5.2:2190314-2190333	MS.gene21734:CDS	25.0%
!	TGATAATCTACTTTACATCT+GGG	+	chr5.2:2190843-2190862	MS.gene21734:CDS	25.0%
!	TTACCTCAATTTATTAGACT+GGG	+	chr5.2:2190315-2190334	MS.gene21734:CDS	25.0%
!	TTAGACTACTAATCCAATTA+TGG	-	chr5.2:2189303-2189322	None:intergenic	25.0%
!!	AAATGTTCTTACATTTTCCT+TGG	-	chr5.2:2189204-2189223	None:intergenic	25.0%
!!	AGGAATTTGATAGTTCATAA+AGG	-	chr5.2:2190576-2190595	None:intergenic	25.0%
!!	TAGTTCATAAAGGTGTATTA+AGG	-	chr5.2:2190566-2190585	None:intergenic	25.0%
!!!	AATGAGTTTTGAAAATCAAG+TGG	+	chr5.2:2190017-2190036	MS.gene21734:intron	25.0%
!!!	ATGAGTTTTGAAAATCAAGT+GGG	+	chr5.2:2190018-2190037	MS.gene21734:intron	25.0%
!!!	GGGATTTTTAATTTTCGAAT+TGG	+	chr5.2:2189391-2189410	MS.gene21734:intron	25.0%
!!!	TCATTGTTATTTTGGTTCTT+TGG	+	chr5.2:2189967-2189986	MS.gene21734:intron	25.0%
	ACATTGTTGAAACATACAGA+GGG	+	chr5.2:2190755-2190774	MS.gene21734:CDS	30.0%
	CACTTCAAGAGTTCTTTATA+TGG	+	chr5.2:2191188-2191207	MS.gene21734:CDS	30.0%
	CAGATTTATACCGAATAGAT+CGG	+	chr5.2:2189657-2189676	MS.gene21734:CDS	30.0%
	CTTAGTTAAAAGGGTTTGTA+TGG	+	chr5.2:2190061-2190080	MS.gene21734:intron	30.0%
	TACCTCAATTTATTAGACTG+GGG	+	chr5.2:2190316-2190335	MS.gene21734:CDS	30.0%
	TTGCCAACATTATTCTTGTT+TGG	+	chr5.2:2190602-2190621	MS.gene21734:intron	30.0%
!	ACTTTGGAAATATCCTTCAA+TGG	+	chr5.2:2191140-2191159	MS.gene21734:CDS	30.0%
!!	TCTTAGTTGAAAGTGTTGTA+TGG	+	chr5.2:2190185-2190204	MS.gene21734:intron	30.0%
!!!	TTTAGTGTTTTGGCCATAAT+TGG	+	chr5.2:2189287-2189306	MS.gene21734:intron	30.0%
	AAGATAAAACCATAAAGCCC+AGG	+	chr5.2:2189890-2189909	MS.gene21734:CDS	35.0%
	AAGGTGTATTAAGGTTAACC+AGG	-	chr5.2:2190557-2190576	None:intergenic	35.0%
	AATAATGTTGGCAAACTAGC+AGG	-	chr5.2:2190596-2190615	None:intergenic	35.0%
	AATGTTCTTGCAATAGCACT+TGG	+	chr5.2:2190346-2190365	MS.gene21734:CDS	35.0%
	ATATCCTTCAATGGTGAAGA+TGG	+	chr5.2:2191149-2191168	MS.gene21734:CDS	35.0%
	CACATTGTTGAAACATACAG+AGG	+	chr5.2:2190754-2190773	MS.gene21734:CDS	35.0%
	CAGAGTCTTTAAACATGAAC+AGG	+	chr5.2:2189785-2189804	MS.gene21734:CDS	35.0%
	GCTATGGAACAAGAATGAAA+GGG	+	chr5.2:2191077-2191096	MS.gene21734:CDS	35.0%
	GTTCTTGAAAGCTAGAATTC+TGG	-	chr5.2:2189812-2189831	None:intergenic	35.0%
	TAGATTATCATTACCTCCAC+TGG	-	chr5.2:2190833-2190852	None:intergenic	35.0%
	TATTTGTGGGATGCATCAAA+TGG	+	chr5.2:2190379-2190398	MS.gene21734:CDS	35.0%
	TCATGAGGAATCAAATCAAC+TGG	-	chr5.2:2189847-2189866	None:intergenic	35.0%
	TGCTATGGAACAAGAATGAA+AGG	+	chr5.2:2191076-2191095	MS.gene21734:CDS	35.0%
	TGGAAACACAGTGTATTTGT+GGG	+	chr5.2:2190366-2190385	MS.gene21734:CDS	35.0%
	TTCATTCTTGTTCCATAGCA+GGG	-	chr5.2:2191076-2191095	None:intergenic	35.0%
	TTCCCCAGTCTAATAAATTG+AGG	-	chr5.2:2190321-2190340	None:intergenic	35.0%
	TTGGAAACACAGTGTATTTG+TGG	+	chr5.2:2190365-2190384	MS.gene21734:CDS	35.0%
	TTTCATTCTTGTTCCATAGC+AGG	-	chr5.2:2191077-2191096	None:intergenic	35.0%
	TTTCGATTATGCACACTACA+TGG	+	chr5.2:2189687-2189706	MS.gene21734:CDS	35.0%
!	AACAGGTTTTTACACTTACC+TGG	+	chr5.2:2190536-2190555	MS.gene21734:intron	35.0%
!	AAGAAGTTTGCGGATTGAAA+TGG	+	chr5.2:2190785-2190804	MS.gene21734:CDS	35.0%
!	AAGTGCTATTGCAAGAACAT+TGG	-	chr5.2:2190347-2190366	None:intergenic	35.0%
!	GATTAGTTGGCTATCTGATT+AGG	+	chr5.2:2190124-2190143	MS.gene21734:intron	35.0%
!!	AAAACGTGATTGGGTTTTGA+TGG	-	chr5.2:2189150-2189169	None:intergenic	35.0%
!!	TGTGCTAGTTGATGATTAGT+TGG	+	chr5.2:2190111-2190130	MS.gene21734:intron	35.0%
!!	TTGATTTGTTTGTTGCCAGT+TGG	+	chr5.2:2189633-2189652	MS.gene21734:intron	35.0%
!!	TTGTTTTTCAGACTTCTGAG+AGG	+	chr5.2:2190260-2190279	MS.gene21734:intron	35.0%
!!!	TTGAGGTTTTGGAATGCTTT+TGG	+	chr5.2:2191261-2191280	MS.gene21734:CDS	35.0%
	AAATCCATCGCCGATCTATT+CGG	-	chr5.2:2189670-2189689	None:intergenic	40.0%
	AACAACCACATACTCACAAC+CGG	+	chr5.2:2190685-2190704	MS.gene21734:CDS	40.0%
	AATAATTCGCCTGGGCTTTA+TGG	-	chr5.2:2189902-2189921	None:intergenic	40.0%
	AGACCATACATCTGCAGTAA+AGG	+	chr5.2:2190918-2190937	MS.gene21734:CDS	40.0%
	AGATTTCAGTAACACACCTG+AGG	-	chr5.2:2189925-2189944	None:intergenic	40.0%
	ATCAAATCAACTGGAGTAGG+TGG	-	chr5.2:2189838-2189857	None:intergenic	40.0%
	CACAGAATCAGCTAACACTT+TGG	+	chr5.2:2191124-2191143	MS.gene21734:CDS	40.0%
	CTGAAGAGGAAAACGTGATT+GGG	-	chr5.2:2189159-2189178	None:intergenic	40.0%
	CTGCAGTTGAGAACTTTGAA+AGG	+	chr5.2:2190628-2190647	MS.gene21734:intron	40.0%
	CTTACAGAGAAAGAGTTCCA+AGG	+	chr5.2:2189184-2189203	MS.gene21734:CDS	40.0%
	GATGATGAAGTAAGCTCATG+AGG	-	chr5.2:2189862-2189881	None:intergenic	40.0%
	GGAATCAAATCAACTGGAGT+AGG	-	chr5.2:2189841-2189860	None:intergenic	40.0%
	GGATACAGCTTCAGATAAAC+AGG	+	chr5.2:2190519-2190538	MS.gene21734:CDS	40.0%
	TAATAGTCATCCACAAGGTC+TGG	-	chr5.2:2190298-2190317	None:intergenic	40.0%
	TCGGTATAAATCTGTCCAAC+TGG	-	chr5.2:2189651-2189670	None:intergenic	40.0%
	TGAGGTAATAGTCATCCACA+AGG	-	chr5.2:2190303-2190322	None:intergenic	40.0%
!	AGTTTGCGGATTGAAATGGT+CGG	+	chr5.2:2190789-2190808	MS.gene21734:CDS	40.0%
!	CAGACGAAACATTGAGGTTT+TGG	+	chr5.2:2191250-2191269	MS.gene21734:CDS	40.0%
!	CAGTTGAGAACTTTGAAAGG+TGG	+	chr5.2:2190631-2190650	MS.gene21734:intron	40.0%
!	GAACTTTGAAAGGTGGTCAT+AGG	+	chr5.2:2190638-2190657	MS.gene21734:CDS	40.0%
!	GCGATCGAACAATCAAGTTT+TGG	+	chr5.2:2190995-2191014	MS.gene21734:CDS	40.0%
!	TTGGGGGTTTTCTTAGATTG+TGG	+	chr5.2:2189353-2189372	MS.gene21734:intron	40.0%
!!!	AAGTTTTGGAATACCCACAC+CGG	+	chr5.2:2191009-2191028	MS.gene21734:CDS	40.0%
	AACGGTCAACAATTAGCCAG+TGG	+	chr5.2:2190814-2190833	MS.gene21734:CDS	45.0%
	AATGGCTCAGCTCTTGCTTT+CGG	-	chr5.2:2191315-2191334	None:intergenic	45.0%
	ACACTACATGGTGACAGAAG+GGG	+	chr5.2:2189699-2189718	MS.gene21734:CDS	45.0%
	ACCTGAGGAATAATTCGCCT+GGG	-	chr5.2:2189910-2189929	None:intergenic	45.0%
	ATGCGGCTCACATGTGAAAA+TGG	-	chr5.2:2191333-2191352	None:intergenic	45.0%
	CACACTACATGGTGACAGAA+GGG	+	chr5.2:2189698-2189717	MS.gene21734:CDS	45.0%
	CAGTAGCTAGCAAATTCCCT+TGG	-	chr5.2:2190963-2190982	None:intergenic	45.0%
	CCATAGCAGGGAACATACTT+GGG	-	chr5.2:2191064-2191083	None:intergenic	45.0%
	CCCAACTCACAGATGTGATA+GGG	-	chr5.2:2190444-2190463	None:intergenic	45.0%
	CCCTATCACATCTGTGAGTT+GGG	+	chr5.2:2190441-2190460	MS.gene21734:CDS	45.0%
	CCTGAAGAGGAAAACGTGAT+TGG	-	chr5.2:2189160-2189179	None:intergenic	45.0%
	CTCTGTAAGTGTTCCTGAAG+AGG	-	chr5.2:2189173-2189192	None:intergenic	45.0%
	GAGCCTTTACTGCAGATGTA+TGG	-	chr5.2:2190924-2190943	None:intergenic	45.0%
	GCTAGCAAATTCCCTTGGAA+TGG	-	chr5.2:2190958-2190977	None:intergenic	45.0%
	GGATTGAAATGGTCGGCTAA+CGG	+	chr5.2:2190796-2190815	MS.gene21734:CDS	45.0%
	GGGCATGAACAAGAAGTTTG+CGG	+	chr5.2:2190775-2190794	MS.gene21734:CDS	45.0%
	GTTACCGTCGAAGAAGAAGA+CGG	+	chr5.2:2190418-2190437	MS.gene21734:CDS	45.0%
	TATACCGAATAGATCGGCGA+TGG	+	chr5.2:2189663-2189682	MS.gene21734:CDS	45.0%
	TCCATAGCAGGGAACATACT+TGG	-	chr5.2:2191065-2191084	None:intergenic	45.0%
	TCTGCCATCTTCACCATTGA+AGG	-	chr5.2:2191156-2191175	None:intergenic	45.0%
!	AATTTTACCGTCCATCCCTC+CGG	-	chr5.2:2190707-2190726	None:intergenic	45.0%
!	CCAATCACGTTTTCCTCTTC+AGG	+	chr5.2:2189157-2189176	MS.gene21734:CDS	45.0%
!	CTACTTTACATCTGGGACAG+AGG	+	chr5.2:2190850-2190869	MS.gene21734:CDS	45.0%
!	GCAGCTTTTGCTACTACTTC+TGG	-	chr5.2:2191291-2191310	None:intergenic	45.0%
!	TCAGACTTCTGAGAGGACTT+TGG	+	chr5.2:2190267-2190286	MS.gene21734:intron	45.0%
!!	AATTTGCTAGCTACTGGTGG+AGG	+	chr5.2:2190967-2190986	MS.gene21734:CDS	45.0%
!!	ACAACTCTGAAGTGCAGCTA+TGG	+	chr5.2:2190497-2190516	MS.gene21734:CDS	45.0%
!!	ACGTGATTGGGTTTTGATGG+CGG	-	chr5.2:2189147-2189166	None:intergenic	45.0%
!!	CAACTCTGAAGTGCAGCTAT+GGG	+	chr5.2:2190498-2190517	MS.gene21734:CDS	45.0%
!!	CAAGGGAATTTGCTAGCTAC+TGG	+	chr5.2:2190961-2190980	MS.gene21734:CDS	45.0%
!!	CTTCTAGTTCTCCTACACAG+TGG	+	chr5.2:2190881-2190900	MS.gene21734:CDS	45.0%
!!	TTGCTTGGTGTCCATTCCAA+GGG	+	chr5.2:2190944-2190963	MS.gene21734:CDS	45.0%
	AACCACATACTCACAACCGG+AGG	+	chr5.2:2190688-2190707	MS.gene21734:CDS	50.0%
	AACCGAGTTCAAGCATGCAC+CGG	-	chr5.2:2191031-2191050	None:intergenic	50.0%
	ACCACATACTCACAACCGGA+GGG	+	chr5.2:2190689-2190708	MS.gene21734:CDS	50.0%
	AGGGAGTTACTCAGCTCTCA+TGG	+	chr5.2:2191096-2191115	MS.gene21734:CDS	50.0%
	ATGCCAATATGACGCCCATC+AGG	-	chr5.2:2190469-2190488	None:intergenic	50.0%
	ATGGTGACAGAAGGGGCTAA+AGG	+	chr5.2:2189706-2189725	MS.gene21734:CDS	50.0%
	CACCTGAGGAATAATTCGCC+TGG	-	chr5.2:2189911-2189930	None:intergenic	50.0%
	CAGCAGCAGACGAAACATTG+AGG	+	chr5.2:2191244-2191263	MS.gene21734:CDS	50.0%
	CCCAAGTATGTTCCCTGCTA+TGG	+	chr5.2:2191061-2191080	MS.gene21734:CDS	50.0%
	CTGCAGTAAAGGCTCTTGCT+TGG	+	chr5.2:2190929-2190948	MS.gene21734:CDS	50.0%
	GACAGAAGGGGCTAAAGGTA+AGG	+	chr5.2:2189711-2189730	MS.gene21734:CDS	50.0%
	GATGGGCGTCATATTGGCAT+TGG	+	chr5.2:2190469-2190488	MS.gene21734:CDS	50.0%
	GCACACTACATGGTGACAGA+AGG	+	chr5.2:2189697-2189716	MS.gene21734:CDS	50.0%
	GCCCAACTCACAGATGTGAT+AGG	-	chr5.2:2190445-2190464	None:intergenic	50.0%
	GCCCTATCACATCTGTGAGT+TGG	+	chr5.2:2190440-2190459	MS.gene21734:CDS	50.0%
	GCTTGAACTCGGTTGACACT+GGG	+	chr5.2:2191037-2191056	MS.gene21734:CDS	50.0%
	GGTCAACAATTAGCCAGTGG+AGG	+	chr5.2:2190817-2190836	MS.gene21734:CDS	50.0%
	GTGAAGATGGCAGAGCTCAA+TGG	+	chr5.2:2191162-2191181	MS.gene21734:CDS	50.0%
	TAGCTTCCTGTACGCTTCTC+TGG	-	chr5.2:2189761-2189780	None:intergenic	50.0%
	TCTTTATATGGCGCAGAGCC+CGG	+	chr5.2:2191200-2191219	MS.gene21734:CDS	50.0%
	TGCTTGAACTCGGTTGACAC+TGG	+	chr5.2:2191036-2191055	MS.gene21734:CDS	50.0%
	TTCCTGTACGCTTCTCTGGA+AGG	-	chr5.2:2189757-2189776	None:intergenic	50.0%
!	TCCTGTACGCTTCTCTGGAA+GGG	-	chr5.2:2189756-2189775	None:intergenic	50.0%
!!	CGTGATTGGGTTTTGATGGC+GGG	-	chr5.2:2189146-2189165	None:intergenic	50.0%
!!	CTTGCTTGGTGTCCATTCCA+AGG	+	chr5.2:2190943-2190962	MS.gene21734:CDS	50.0%
!!	GGGAATTTGCTAGCTACTGG+TGG	+	chr5.2:2190964-2190983	MS.gene21734:CDS	50.0%
	AGCCTGTGAAGCCACTGTGT+AGG	-	chr5.2:2190895-2190914	None:intergenic	55.0%
	AGCGAGTAGGGTCTTTGGCA+TGG	+	chr5.2:2190662-2190681	MS.gene21734:CDS	55.0%
	AGGGCCGTCTTCTTCTTCGA+CGG	-	chr5.2:2190425-2190444	None:intergenic	55.0%
	CACCGGTGCATGCTTGAACT+CGG	+	chr5.2:2191026-2191045	MS.gene21734:CDS	55.0%
	CATACTCACAACCGGAGGGA+TGG	+	chr5.2:2190693-2190712	MS.gene21734:CDS	55.0%
	CTCCTACACAGTGGCTTCAC+AGG	+	chr5.2:2190890-2190909	MS.gene21734:CDS	55.0%
	GCCCAGGCGAATTATTCCTC+AGG	+	chr5.2:2189906-2189925	MS.gene21734:CDS	55.0%
	GTGGTCATAGGCAGCGAGTA+GGG	+	chr5.2:2190650-2190669	MS.gene21734:CDS	55.0%
	GTTGCCACTGTGCAACCATC+CGG	-	chr5.2:2191222-2191241	None:intergenic	55.0%
	TAGGCAGCGAGTAGGGTCTT+TGG	+	chr5.2:2190657-2190676	MS.gene21734:CDS	55.0%
	TCCCTCCGGTTGTGAGTATG+TGG	-	chr5.2:2190693-2190712	None:intergenic	55.0%
	TTGCCACTGTGCAACCATCC+GGG	-	chr5.2:2191221-2191240	None:intergenic	55.0%
	TTTGGACGCTCCAGACCTTG+TGG	+	chr5.2:2190285-2190304	MS.gene21734:CDS	55.0%
!	AGTTCAAGCATGCACCGGTG+TGG	-	chr5.2:2191026-2191045	None:intergenic	55.0%
!	CCTGTACGCTTCTCTGGAAG+GGG	-	chr5.2:2189755-2189774	None:intergenic	55.0%
!	CTGTGAGTTGGGCTCCTGAT+GGG	+	chr5.2:2190452-2190471	MS.gene21734:CDS	55.0%
!	GCTCCTGATGGGCGTCATAT+TGG	+	chr5.2:2190463-2190482	MS.gene21734:CDS	55.0%
!	GTTCAAGCATGCACCGGTGT+GGG	-	chr5.2:2191025-2191044	None:intergenic	55.0%
!	TCTGTGAGTTGGGCTCCTGA+TGG	+	chr5.2:2190451-2190470	MS.gene21734:CDS	55.0%
	CTAGCTACTGGTGGAGGCAG+TGG	+	chr5.2:2190973-2190992	MS.gene21734:CDS	60.0%
	CTCACAACCGGAGGGATGGA+CGG	+	chr5.2:2190697-2190716	MS.gene21734:CDS	60.0%
	GGTGGTCATAGGCAGCGAGT+AGG	+	chr5.2:2190649-2190668	MS.gene21734:CDS	60.0%
	TATATGGCGCAGAGCCCGGA+TGG	+	chr5.2:2191204-2191223	MS.gene21734:CDS	60.0%
!	CCCCTTCCAGAGAAGCGTAC+AGG	+	chr5.2:2189752-2189771	MS.gene21734:CDS	60.0%
!	CTGGAAGGGGAGCACACTTC+TGG	-	chr5.2:2189742-2189761	None:intergenic	60.0%
	GAGCCCGGATGGTTGCACAG+TGG	+	chr5.2:2191215-2191234	MS.gene21734:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	2189119	2191359	2189119	ID=MS.gene21734
chr5.2	mRNA	2189119	2191359	2189119	ID=MS.gene21734.t1;Parent=MS.gene21734
chr5.2	exon	2189119	2189211	2189119	ID=MS.gene21734.t1.exon1;Parent=MS.gene21734.t1
chr5.2	CDS	2189119	2189211	2189119	ID=cds.MS.gene21734.t1;Parent=MS.gene21734.t1
chr5.2	exon	2189652	2189927	2189652	ID=MS.gene21734.t1.exon2;Parent=MS.gene21734.t1
chr5.2	CDS	2189652	2189927	2189652	ID=cds.MS.gene21734.t1;Parent=MS.gene21734.t1
chr5.2	exon	2190271	2190540	2190271	ID=MS.gene21734.t1.exon3;Parent=MS.gene21734.t1
chr5.2	CDS	2190271	2190540	2190271	ID=cds.MS.gene21734.t1;Parent=MS.gene21734.t1
chr5.2	exon	2190634	2191359	2190634	ID=MS.gene21734.t1.exon4;Parent=MS.gene21734.t1
chr5.2	CDS	2190634	2191359	2190634	ID=cds.MS.gene21734.t1;Parent=MS.gene21734.t1

Gene Sequence

>MS.gene21734

ATGGCTTCAGGTTCTTTGAGTTCTCCCGCCATCAAAACCCAATCACGTTTTCCTCTTCAGGAACACTTACAGAGAAAGAGTTCCAAGGAAAATGTAAGAACATTTTTCAATTATTCAGATCTTTCAAAATTAGGGTTTTAATGTAAATTGGGATTTTAATTATCAAATTTTAGTGTTTTGGCCATAATTGGATTAGTAGTCTAAAAACTGTGATAAAGAATAAATTTTGAAATTTTGGGGGTTTTCTTAGATTGTGAATAAATTTTAAAATTGGGATTTTTAATTTTCGAATTGGGGGTTTTCTTAGATTGTGGATTAATTTTGAAAATATGTTAAAAAAGAATGAATTTTGACATTATGTCATGAAAAAGAATGAATTTTGACATTATGTCATGAAAAAGAATGAATTTTGAAATTATGCTAAACTTTTTATTGACTGAGTTGAAATTATGATATGATAAGTTGATAATATTCTGAATTTGTTTATGAAAATCAAGTATTGATTCTTTATTGTTTGATTTGTTTGTTGCCAGTTGGACAGATTTATACCGAATAGATCGGCGATGGATTTCGATTATGCACACTACATGGTGACAGAAGGGGCTAAAGGTAAGGAAAATCCAGAAGTGTGCTCCCCTTCCAGAGAAGCGTACAGGAAGCTACTTGCAGAGTCTTTAAACATGAACAGGACCAGAATTCTAGCTTTCAAGAACAAGCCACCTACTCCAGTTGATTTGATTCCTCATGAGCTTACTTCATCATCTCTTCAACAAGATAAAACCATAAAGCCCAGGCGAATTATTCCTCAGGTGTGTTACTGAAATCTGATTGATATTCCTTATGTTTTATCATTGTTATTTTGGTTCTTTGGTTGCATGAACTCCAAATTTAAATTTTGAATGAGTTTTGAAAATCAAGTGGGTCTGAGTTGTTTTATTAATTCTTAGTTAAAAGGGTTTGTATGGTGAATGAAATTCAATATTTTGCAATTTTGTGCTAGTTGATGATTAGTTGGCTATCTGATTAGGTTTTTGAAATTCAAATGGATCTGAGTTGTTTTCTTAATTCTTAGTTGAAAGTGTTGTATGGTATAAACTGTAAACCATAATATTTTGCAATTTTGTGCTAATTGATTGATATGTTGTTTTTCAGACTTCTGAGAGGACTTTGGACGCTCCAGACCTTGTGGATGACTATTACCTCAATTTATTAGACTGGGGAAGCGCCAATGTTCTTGCAATAGCACTTGGAAACACAGTGTATTTGTGGGATGCATCAAATGGTTCTACTTCAGAACTTGTTACCGTCGAAGAAGAAGACGGCCCTATCACATCTGTGAGTTGGGCTCCTGATGGGCGTCATATTGGCATTGGTTTGAACAACTCTGAAGTGCAGCTATGGGATACAGCTTCAGATAAACAGGTTTTTACACTTACCTGGTTAACCTTAATACACCTTTATGAACTATCAAATTCCTGCTAGTTTGCCAACATTATTCTTGTTTGGTTTCTGCAGTTGAGAACTTTGAAAGGTGGTCATAGGCAGCGAGTAGGGTCTTTGGCATGGAACAACCACATACTCACAACCGGAGGGATGGACGGTAAAATTATCAATAATGATGTAAGAATTAGAGCACACATTGTTGAAACATACAGAGGGCATGAACAAGAAGTTTGCGGATTGAAATGGTCGGCTAACGGTCAACAATTAGCCAGTGGAGGTAATGATAATCTACTTTACATCTGGGACAGAGGTACATCAACTTCTAGTTCTCCTACACAGTGGCTTCACAGGCTTGAAGACCATACATCTGCAGTAAAGGCTCTTGCTTGGTGTCCATTCCAAGGGAATTTGCTAGCTACTGGTGGAGGCAGTGGCGATCGAACAATCAAGTTTTGGAATACCCACACCGGTGCATGCTTGAACTCGGTTGACACTGGGTCCCAAGTATGTTCCCTGCTATGGAACAAGAATGAAAGGGAGTTACTCAGCTCTCATGGTTTTACACAGAATCAGCTAACACTTTGGAAATATCCTTCAATGGTGAAGATGGCAGAGCTCAATGGTCACACTTCAAGAGTTCTTTATATGGCGCAGAGCCCGGATGGTTGCACAGTGGCAACAGCAGCAGCAGACGAAACATTGAGGTTTTGGAATGCTTTTGGCACTCCAGAAGTAGTAGCAAAAGCTGCACCGAAAGCAAGAGCTGAGCCATTTTCACATGTGAGCCGCATTCGATAA

Protein sequence

>MS.gene21734.t1

MASGSLSSPAIKTQSRFPLQEHLQRKSSKENLDRFIPNRSAMDFDYAHYMVTEGAKGKENPEVCSPSREAYRKLLAESLNMNRTRILAFKNKPPTPVDLIPHELTSSSLQQDKTIKPRRIIPQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWDASNGSTSELVTVEEEDGPITSVSWAPDGRHIGIGLNNSEVQLWDTASDKQLRTLKGGHRQRVGSLAWNNHILTTGGMDGKIINNDVRIRAHIVETYRGHEQEVCGLKWSANGQQLASGGNDNLLYIWDRGTSTSSSPTQWLHRLEDHTSAVKALAWCPFQGNLLATGGGSGDRTIKFWNTHTGACLNSVDTGSQVCSLLWNKNERELLSSHGFTQNQLTLWKYPSMVKMAELNGHTSRVLYMAQSPDGCTVATAAADETLRFWNAFGTPEVVAKAAPKARAEPFSHVSRIR