AlfalfaGEDB | MS.gene024753

M. sativa cultivar XinJiangDaYe / MS.gene024753

KEGG: K03363
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00400.35 PF12894.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene024753.t1	XP_003610876.1	99.1	454	4	0	1	454	1	454	1.50E-265	924.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene024753.t1	Q9SZA4	79.8	450	89	2	7	454	8	457	2.4e-216	752.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene024753.t1	G7K6J6	99.1	454	4	0	1	454	1	454	1.1e-265	924.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene060495	MS.gene024753	PPI
MS.gene89986	MS.gene024753	PPI
MS.gene069995	MS.gene024753	PPI
MS.gene024753	MS.gene26019	PPI
MS.gene024753	MS.gene050272	PPI
MS.gene049022	MS.gene024753	PPI
MS.gene69687	MS.gene024753	PPI
MS.gene024753	MS.gene98540	PPI
MS.gene024753	MS.gene30176	PPI
MS.gene024753	MS.gene51258	PPI
MS.gene69965	MS.gene024753	PPI
MS.gene024753	MS.gene32723	PPI
MS.gene024753	MS.gene067321	PPI
MS.gene024753	MS.gene29842	PPI
MS.gene024753	MS.gene053363	PPI
MS.gene024753	MS.gene55883	PPI
MS.gene82740	MS.gene024753	PPI
MS.gene024753	MS.gene75493	PPI
MS.gene78281	MS.gene024753	PPI
MS.gene55882	MS.gene024753	PPI
MS.gene024753	MS.gene32726	PPI
MS.gene024753	MS.gene71122	PPI
MS.gene067322	MS.gene024753	PPI
MS.gene060496	MS.gene024753	PPI
MS.gene024753	MS.gene85534	PPI
MS.gene69966	MS.gene024753	PPI
MS.gene24806	MS.gene024753	PPI
MS.gene071113	MS.gene024753	PPI
MS.gene52355	MS.gene024753	PPI
MS.gene024753	MS.gene014321	PPI
MS.gene024753	MS.gene51262	PPI
MS.gene024753	MS.gene75059	PPI
MS.gene024753	MS.gene24951	PPI
MS.gene027071	MS.gene024753	PPI
MS.gene024753	MS.gene23881	PPI
MS.gene024753	MS.gene26672	PPI
MS.gene024753	MS.gene06176	PPI
MS.gene024753	MS.gene014931	PPI
MS.gene024753	MS.gene062662	PPI
MS.gene024753	MS.gene21888	PPI
MS.gene024753	MS.gene97379	PPI
MS.gene024753	MS.gene066500	PPI
MS.gene010910	MS.gene024753	PPI
MS.gene066464	MS.gene024753	PPI
MS.gene063222	MS.gene024753	PPI
MS.gene07706	MS.gene024753	PPI
MS.gene027074	MS.gene024753	PPI
MS.gene024753	MS.gene014419	PPI
MS.gene024753	MS.gene022109	PPI
MS.gene024753	MS.gene62819	PPI
MS.gene010912	MS.gene024753	PPI
MS.gene024753	MS.gene65775	PPI
MS.gene024753	MS.gene80163	PPI
MS.gene23811	MS.gene024753	PPI
MS.gene23810	MS.gene024753	PPI
MS.gene46611	MS.gene024753	PPI
MS.gene92674	MS.gene024753	PPI
MS.gene024753	MS.gene033401	PPI
MS.gene024753	MS.gene33377	PPI
MS.gene51335	MS.gene024753	PPI
MS.gene82021	MS.gene024753	PPI
MS.gene024753	MS.gene045937	PPI
MS.gene81658	MS.gene024753	PPI
MS.gene024753	MS.gene027072	PPI
MS.gene84465	MS.gene024753	PPI
MS.gene024753	MS.gene068273	PPI
MS.gene037950	MS.gene024753	PPI
MS.gene024753	MS.gene06177	PPI
MS.gene024753	MS.gene51278	PPI
MS.gene024753	MS.gene052188	PPI
MS.gene024753	MS.gene87218	PPI
MS.gene068249	MS.gene024753	PPI
MS.gene066046	MS.gene024753	PPI
MS.gene64441	MS.gene024753	PPI
MS.gene30176	MS.gene024753	PPI
MS.gene024753	MS.gene025229	PPI
MS.gene024753	MS.gene064862	PPI
MS.gene024753	MS.gene37226	PPI
MS.gene024753	MS.gene049021	PPI
MS.gene024753	MS.gene043742	PPI
MS.gene024753	MS.gene027441	PPI
MS.gene024753	MS.gene022502	PPI
MS.gene024753	MS.gene050457	PPI
MS.gene024753	MS.gene30177	PPI
MS.gene024753	MS.gene84466	PPI
MS.gene024753	MS.gene70008	PPI
MS.gene024753	MS.gene008833	PPI
MS.gene29768	MS.gene024753	PPI
MS.gene024753	MS.gene064435	PPI
MS.gene20161	MS.gene024753	PPI
MS.gene024753	MS.gene82740	PPI
MS.gene24922	MS.gene024753	PPI
MS.gene33406	MS.gene024753	PPI
MS.gene024753	MS.gene48655	PPI
MS.gene066467	MS.gene024753	PPI
MS.gene21734	MS.gene024753	PPI
MS.gene024753	MS.gene81663	PPI
MS.gene024753	MS.gene027073	PPI
MS.gene024753	MS.gene34712	PPI
MS.gene064035	MS.gene024753	PPI
MS.gene024753	MS.gene65463	PPI
MS.gene77885	MS.gene024753	PPI
MS.gene36842	MS.gene024753	PPI
MS.gene024753	MS.gene037184	PPI
MS.gene99554	MS.gene024753	PPI
MS.gene058128	MS.gene024753	PPI
MS.gene41291	MS.gene024753	PPI
MS.gene024753	MS.gene067705	PPI
MS.gene011448	MS.gene024753	PPI
MS.gene066501	MS.gene024753	PPI
MS.gene66342	MS.gene024753	PPI
MS.gene63085	MS.gene024753	PPI
MS.gene056455	MS.gene024753	PPI
MS.gene024753	MS.gene027075	PPI
MS.gene030294	MS.gene024753	PPI
MS.gene024753	MS.gene66343	PPI
MS.gene064762	MS.gene024753	PPI
MS.gene024753	MS.gene99973	PPI
MS.gene91851	MS.gene024753	PPI
MS.gene024753	MS.gene30174	PPI
MS.gene024753	MS.gene26021	PPI
MS.gene35613	MS.gene024753	PPI
MS.gene024753	MS.gene050456	PPI
MS.gene039682	MS.gene024753	PPI
MS.gene024753	MS.gene91775	PPI
MS.gene024753	MS.gene25515	PPI
MS.gene024753	MS.gene34651	PPI
MS.gene82020	MS.gene024753	PPI
MS.gene024753	MS.gene050270	PPI
MS.gene71204	MS.gene024753	PPI
MS.gene30172	MS.gene024753	PPI
MS.gene024753	MS.gene052956	PPI
MS.gene23852	MS.gene024753	PPI
MS.gene97943	MS.gene024753	PPI
MS.gene024753	MS.gene81660	PPI
MS.gene67157	MS.gene024753	PPI
MS.gene90626	MS.gene024753	PPI
MS.gene69396	MS.gene024753	PPI
MS.gene024753	MS.gene004844	PPI
MS.gene42253	MS.gene024753	PPI
MS.gene024753	MS.gene99588	PPI
MS.gene012203	MS.gene024753	PPI
MS.gene25244	MS.gene024753	PPI
MS.gene024753	MS.gene038519	PPI
MS.gene024753	MS.gene27223	PPI
MS.gene024753	MS.gene045133	PPI
MS.gene024753	MS.gene042783	PPI
MS.gene024753	MS.gene20160	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene024753.t1	MTR_5g008010	99.119	454	4	0	1	454	1	454	0.0	937
MS.gene024753.t1	MTR_3g051690	91.503	459	34	4	1	454	1	459	0.0	872
MS.gene024753.t1	MTR_7g106870	75.223	448	105	5	12	454	11	457	0.0	707
MS.gene024753.t1	MTR_7g451830	66.966	445	126	7	15	454	3	431	0.0	591
MS.gene024753.t1	MTR_7g047640	66.966	445	126	7	15	454	3	431	0.0	591
MS.gene024753.t1	MTR_7g057540	65.011	443	135	7	14	454	2	426	0.0	563
MS.gene024753.t1	MTR_7g055590	64.932	442	135	7	14	453	2	425	0.0	561
MS.gene024753.t1	MTR_7g053100	61.607	448	147	8	17	454	4	436	0.0	537
MS.gene024753.t1	MTR_7g034625	71.160	319	90	1	124	440	13	331	1.14e-172	488
MS.gene024753.t1	MTR_2g100540	51.945	437	160	13	25	454	4	397	2.69e-135	395
MS.gene024753.t1	MTR_2g100460	56.322	348	133	6	107	452	52	382	2.03e-129	380
MS.gene024753.t1	MTR_2g060710	45.388	412	202	8	33	430	26	428	9.05e-123	365
MS.gene024753.t1	MTR_3g067940	45.012	411	201	7	33	429	57	456	2.79e-115	347
MS.gene024753.t1	MTR_4g102510	41.611	447	218	9	11	433	29	456	4.74e-113	341
MS.gene024753.t1	MTR_2g012630	27.386	241	156	9	140	374	18	245	1.42e-17	86.3
MS.gene024753.t1	MTR_2g012630	27.386	241	156	9	140	374	18	245	1.61e-17	85.9
MS.gene024753.t1	MTR_4g415070	61.111	54	21	0	379	432	57	110	6.98e-16	79.0
MS.gene024753.t1	MTR_6g004040	26.891	238	155	9	140	371	19	243	3.61e-15	78.6
MS.gene024753.t1	MTR_5g016370	23.630	292	162	8	143	426	312	550	8.55e-15	77.0
MS.gene024753.t1	MTR_4g095058	26.336	262	168	11	177	427	287	534	2.81e-14	75.5
MS.gene024753.t1	MTR_4g095058	26.336	262	168	11	177	427	285	532	3.23e-14	75.1
MS.gene024753.t1	MTR_1g054300	27.778	252	152	11	182	426	253	481	3.68e-14	74.7
MS.gene024753.t1	MTR_1g054300	25.510	294	156	10	182	428	122	399	1.53e-13	72.8
MS.gene024753.t1	MTR_5g016350	23.410	346	191	11	83	426	201	474	2.15e-13	72.4
MS.gene024753.t1	MTR_5g016350	23.410	346	191	11	83	426	265	538	2.37e-13	72.4
MS.gene024753.t1	MTR_3g106080	24.194	310	192	9	155	434	326	622	1.60e-12	70.1
MS.gene024753.t1	MTR_3g106080	27.273	198	129	7	150	345	434	618	2.80e-12	69.3
MS.gene024753.t1	MTR_1g049000	40.426	94	46	3	200	284	8	100	1.48e-11	63.5
MS.gene024753.t1	MTR_5g022690	27.374	179	118	5	166	342	266	434	2.73e-11	65.9
MS.gene024753.t1	MTR_7g086360	22.857	210	150	4	220	427	53	252	7.15e-11	63.9
MS.gene024753.t1	MTR_1g072140	26.768	198	128	6	150	341	126	312	8.79e-11	63.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene024753.t1	AT4G33270	79.778	450	89	2	7	454	8	457	0.0	759
MS.gene024753.t1	AT4G33260	80.317	442	85	2	15	454	6	447	0.0	737
MS.gene024753.t1	AT4G33260	80.535	411	78	2	15	423	6	416	0.0	701
MS.gene024753.t1	AT5G27570	69.623	451	130	5	7	454	4	450	0.0	642
MS.gene024753.t1	AT5G27080	69.585	434	126	4	23	454	13	442	0.0	623
MS.gene024753.t1	AT5G26900	68.578	436	132	3	20	454	13	444	0.0	612
MS.gene024753.t1	AT5G27945	62.634	372	123	5	95	454	61	428	1.40e-162	466
MS.gene024753.t1	AT4G22910	42.822	411	217	4	33	433	62	464	3.27e-115	347
MS.gene024753.t1	AT5G13840	51.974	304	138	3	126	429	163	458	1.13e-110	335
MS.gene024753.t1	AT5G13840	51.974	304	138	3	126	429	163	458	1.13e-110	335
MS.gene024753.t1	AT4G11920	41.556	450	227	9	5	433	22	456	2.28e-110	334
MS.gene024753.t1	AT1G11160	29.101	189	119	7	159	345	40	215	3.82e-16	81.6
MS.gene024753.t1	AT1G11160	29.101	189	119	7	159	345	40	215	4.21e-16	81.6
MS.gene024753.t1	AT1G11160	29.101	189	119	7	159	345	40	215	4.33e-16	81.6
MS.gene024753.t1	AT5G08390	26.891	238	155	9	140	371	19	243	9.43e-16	80.5
MS.gene024753.t1	AT1G61210	26.201	229	141	8	138	351	6	221	1.50e-15	80.1
MS.gene024753.t1	AT1G61210	26.201	229	141	8	138	351	6	221	1.70e-15	79.7
MS.gene024753.t1	AT1G61210	26.201	229	141	8	138	351	6	221	1.70e-15	79.7
MS.gene024753.t1	AT1G11160	29.341	167	105	6	181	345	18	173	1.96e-15	79.3
MS.gene024753.t1	AT5G23430	27.189	217	140	8	140	351	19	222	1.35e-14	76.6
MS.gene024753.t1	AT5G23430	27.189	217	140	8	140	351	19	222	1.40e-14	76.6
MS.gene024753.t1	AT5G23430	27.189	217	140	8	140	351	19	222	1.40e-14	76.6
MS.gene024753.t1	AT5G52820	27.451	255	152	12	182	428	244	473	7.93e-14	73.9
MS.gene024753.t1	AT5G25150	26.000	300	183	10	155	428	329	615	2.41e-13	72.8
MS.gene024753.t1	AT5G25150	27.551	196	127	7	150	343	433	615	1.96e-11	66.6
MS.gene024753.t1	AT5G25150	26.000	300	183	10	155	428	329	615	2.86e-13	72.4
MS.gene024753.t1	AT5G25150	27.551	196	127	7	150	343	433	615	2.17e-11	66.6
MS.gene024753.t1	AT3G49660	23.922	255	175	9	178	426	71	312	7.45e-13	69.7
MS.gene024753.t1	AT3G49660	25.373	201	125	9	150	341	128	312	9.88e-11	63.2
MS.gene024753.t1	AT5G56130	23.077	208	117	4	259	427	16	219	1.05e-11	66.2
MS.gene024753.t1	AT5G67320	24.221	289	166	12	143	427	330	569	1.54e-11	67.0
MS.gene024753.t1	AT2G41500	25.614	285	153	11	149	430	311	539	1.59e-11	66.6
MS.gene024753.t1	AT2G41500	25.614	285	153	11	149	430	311	539	1.59e-11	66.6
MS.gene024753.t1	AT5G16750	25.238	210	144	7	149	354	32	232	1.89e-11	67.0
MS.gene024753.t1	AT2G32700	24.453	274	180	13	159	426	532	784	4.40e-11	65.9
MS.gene024753.t1	AT2G32700	24.453	274	180	13	159	426	534	786	4.56e-11	65.5
MS.gene024753.t1	AT2G32700	24.453	274	180	13	159	426	534	786	4.56e-11	65.5
MS.gene024753.t1	AT2G32700	24.453	274	180	13	159	426	534	786	4.56e-11	65.5
MS.gene024753.t1	AT2G32700	24.453	274	180	13	159	426	534	786	4.56e-11	65.5
MS.gene024753.t1	AT2G32700	24.453	274	180	13	159	426	534	786	4.56e-11	65.5
MS.gene024753.t1	AT2G32700	24.453	274	180	13	159	426	553	805	5.16e-11	65.5
MS.gene024753.t1	AT5G13480	26.383	235	149	10	182	409	137	354	6.92e-11	65.1
MS.gene024753.t1	AT5G13480	26.383	235	149	10	182	409	114	331	8.15e-11	64.7
MS.gene024753.t1	AT5G13480	26.383	235	149	10	182	409	114	331	8.18e-11	64.7
MS.gene024753.t1	AT5G13480	26.383	235	149	10	182	409	126	343	9.99e-11	64.3

Find 128 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATAATTCGCCTGGGCTTTA+TGG	0.172694	5.3:-2581079	None:intergenic
CAGACGAAACATTGAGGTTT+TGG	0.210103	5.3:+2582431	MS.gene024753:CDS
CTGGAAGGGGAGCACACTTC+TGG	0.268112	5.3:-2580919	None:intergenic
CTCTCTCTAACAATGGCTTC+AGG	0.319079	5.3:+2580287	None:intergenic
ATGCGGCTCACATGTGAAAA+TGG	0.326418	5.3:-2582511	None:intergenic
GTTCTTGAAAGCTAGAATTC+TGG	0.331154	5.3:-2580989	None:intergenic
ATGATAATCTACTTTACATC+TGG	0.357040	5.3:+2582023	MS.gene024753:CDS
TAGCTTCCTGTACGCTTCTC+TGG	0.359002	5.3:-2580938	None:intergenic
AATGGCTCAGCTCTTGCTTT+CGG	0.359726	5.3:-2582493	None:intergenic
GCGATCGAACAATCAAGTTT+TGG	0.364889	5.3:+2582176	MS.gene024753:CDS
CCCTATCACATCTGTGAGTT+GGG	0.371621	5.3:+2581621	MS.gene024753:CDS
CACTTCAAGAGTTCTTTATA+TGG	0.372662	5.3:+2582369	MS.gene024753:CDS
TCCATAGCAGGGAACATACT+TGG	0.385318	5.3:-2582243	None:intergenic
GTTGCCACTGTGCAACCATC+CGG	0.387110	5.3:-2582400	None:intergenic
AAATCCATCGCCGATCTATT+CGG	0.388532	5.3:-2580847	None:intergenic
GCTCCTGATGGGCGTCATAT+TGG	0.389395	5.3:+2581643	MS.gene024753:CDS
CCTGAAGAGGAAAACGTGAT+TGG	0.389938	5.3:-2580337	None:intergenic
GGATACAGCTTCAGATAAAC+AGG	0.391975	5.3:+2581699	MS.gene024753:CDS
CAGAGTCTTTAAACATGAAC+AGG	0.397977	5.3:+2580965	MS.gene024753:CDS
GAACTTTGAAAGGTGGTCAT+AGG	0.402652	5.3:+2581819	MS.gene024753:CDS
ATTACCTCAATTTATTAGAC+TGG	0.406978	5.3:+2581494	MS.gene024753:CDS
CTGAAGAGGAAAACGTGATT+GGG	0.412005	5.3:-2580336	None:intergenic
TCTGCCATCTTCACCATTGA+AGG	0.425176	5.3:-2582334	None:intergenic
AAGAAGTTTGCGGATTGAAA+TGG	0.432752	5.3:+2581966	MS.gene024753:CDS
CCCAAGTATGTTCCCTGCTA+TGG	0.435862	5.3:+2582242	MS.gene024753:CDS
TAGGCAGCGAGTAGGGTCTT+TGG	0.438523	5.3:+2581838	MS.gene024753:CDS
TAATAGTCATCCACAAGGTC+TGG	0.439377	5.3:-2581475	None:intergenic
TTTCATTCTTGTTCCATAGC+AGG	0.442202	5.3:-2582255	None:intergenic
CACAGAATCAGCTAACACTT+TGG	0.446675	5.3:+2582305	MS.gene024753:CDS
GCCCAACTCACAGATGTGAT+AGG	0.447194	5.3:-2581622	None:intergenic
GCCCTATCACATCTGTGAGT+TGG	0.447852	5.3:+2581620	MS.gene024753:CDS
CAAGGGAATTTGCTAGCTAC+TGG	0.450097	5.3:+2582142	MS.gene024753:CDS
CTTGCTTGGTGTCCATTCCA+AGG	0.450852	5.3:+2582124	MS.gene024753:CDS
CTCTGTAAGTGTTCCTGAAG+AGG	0.453227	5.3:-2580350	None:intergenic
TGCTTGAACTCGGTTGACAC+TGG	0.455069	5.3:+2582217	MS.gene024753:CDS
TCATGAGGAATCAAATCAAC+TGG	0.456090	5.3:-2581024	None:intergenic
TTGGAAACACAGTGTATTTG+TGG	0.457657	5.3:+2581545	MS.gene024753:CDS
TCTTTATATGGCGCAGAGCC+CGG	0.458141	5.3:+2582381	MS.gene024753:CDS
CTCCTACACAGTGGCTTCAC+AGG	0.468541	5.3:+2582071	MS.gene024753:CDS
TCAGACTTCTGAGAGGACTT+TGG	0.470401	5.3:+2581447	MS.gene024753:intron
TCCTGTACGCTTCTCTGGAA+GGG	0.470590	5.3:-2580933	None:intergenic
GATGGGCGTCATATTGGCAT+TGG	0.470950	5.3:+2581649	MS.gene024753:CDS
CTAGCTACTGGTGGAGGCAG+TGG	0.480596	5.3:+2582154	MS.gene024753:CDS
TTCATTCTTGTTCCATAGCA+GGG	0.481145	5.3:-2582254	None:intergenic
ACAACTCTGAAGTGCAGCTA+TGG	0.484474	5.3:+2581677	MS.gene024753:CDS
ATGCCAATATGACGCCCATC+AGG	0.488601	5.3:-2581646	None:intergenic
ACTTTGGAAATATCCTTCAA+TGG	0.495344	5.3:+2582321	MS.gene024753:CDS
ATATCCTTCAATGGTGAAGA+TGG	0.498891	5.3:+2582330	MS.gene024753:CDS
AACAACCACATACTCACAAC+CGG	0.499451	5.3:+2581866	MS.gene024753:CDS
TGCTATGGAACAAGAATGAA+AGG	0.505330	5.3:+2582257	MS.gene024753:CDS
TTCCTGTACGCTTCTCTGGA+AGG	0.508481	5.3:-2580934	None:intergenic
TTGCCACTGTGCAACCATCC+GGG	0.511124	5.3:-2582399	None:intergenic
TTGCTTGGTGTCCATTCCAA+GGG	0.513094	5.3:+2582125	MS.gene024753:CDS
TCTGTGAGTTGGGCTCCTGA+TGG	0.513830	5.3:+2581631	MS.gene024753:CDS
CACCGGTGCATGCTTGAACT+CGG	0.514125	5.3:+2582207	MS.gene024753:CDS
TTTCGATTATGCACACTACA+TGG	0.515001	5.3:+2580867	MS.gene024753:CDS
CTGTGAGTTGGGCTCCTGAT+GGG	0.515660	5.3:+2581632	MS.gene024753:CDS
CCCCTTCCAGAGAAGCGTAC+AGG	0.515877	5.3:+2580932	MS.gene024753:CDS
GTTCAAGCATGCACCGGTGT+GGG	0.517001	5.3:-2582203	None:intergenic
AGATCATACATCTGCAGTAA+AGG	0.521203	5.3:+2582099	MS.gene024753:CDS
AGGGAGTTACTCAGCTCTCA+TGG	0.521335	5.3:+2582277	MS.gene024753:CDS
CACCTGAGGAATAATTCGCC+TGG	0.521725	5.3:-2581088	None:intergenic
CACACTACATGGTGACAGAA+GGG	0.522072	5.3:+2580878	MS.gene024753:CDS
GGGCATGAACAAGAAGTTTG+CGG	0.522362	5.3:+2581956	MS.gene024753:CDS
TAGATTATCATTCCCTCCAC+TGG	0.527433	5.3:-2582011	None:intergenic
CTGCAGTTGAGAACTTTGAA+AGG	0.529944	5.3:+2581809	MS.gene024753:intron
TTACCTCAATTTATTAGACT+GGG	0.529967	5.3:+2581495	MS.gene024753:CDS
AATGTTCTTGCAATAGCACT+TGG	0.530924	5.3:+2581526	MS.gene024753:CDS
CCTGTACGCTTCTCTGGAAG+GGG	0.531549	5.3:-2580932	None:intergenic
GCCCAGGCGAATTATTCCTC+AGG	0.535877	5.3:+2581086	MS.gene024753:CDS
GACAGAAGGGGCTAAAGGTA+AGG	0.539789	5.3:+2580891	MS.gene024753:CDS
ATGGTGACAGAAGGGGCTAA+AGG	0.542605	5.3:+2580886	MS.gene024753:CDS
GCTAGCAAATTCCCTTGGAA+TGG	0.543063	5.3:-2582136	None:intergenic
GCTTGAACTCGGTTGACACT+GGG	0.546774	5.3:+2582218	MS.gene024753:CDS
ACCTGAGGAATAATTCGCCT+GGG	0.547062	5.3:-2581087	None:intergenic
TTTGGACGCTCCAGACCTTG+TGG	0.549436	5.3:+2581465	MS.gene024753:CDS
CAACTCTGAAGTGCAGCTAT+GGG	0.552386	5.3:+2581678	MS.gene024753:CDS
CTGCAGTAAAGGCTCTTGCT+TGG	0.552397	5.3:+2582110	MS.gene024753:CDS
GTGGTCATAGGCAGCGAGTA+GGG	0.553407	5.3:+2581831	MS.gene024753:CDS
GGAATCAAATCAACTGGAGT+AGG	0.555155	5.3:-2581018	None:intergenic
GTGAAGATGGCAGAGCTCAA+TGG	0.559588	5.3:+2582343	MS.gene024753:CDS
TGATAATCTACTTTACATCT+GGG	0.563072	5.3:+2582024	MS.gene024753:CDS
CCCAACTCACAGATGTGATA+GGG	0.567907	5.3:-2581621	None:intergenic
GCACACTACATGGTGACAGA+AGG	0.570973	5.3:+2580877	MS.gene024753:CDS
CTACTTTACATCTGGGACAG+AGG	0.574017	5.3:+2582031	MS.gene024753:CDS
TTCCCCAGTCTAATAAATTG+AGG	0.574191	5.3:-2581498	None:intergenic
CATACTCACAACCGGAGGGA+TGG	0.577159	5.3:+2581874	MS.gene024753:CDS
GATGATGAAGTAAGCTCATG+AGG	0.578213	5.3:-2581039	None:intergenic
TCCCTCCGGTTGTGAGTATG+TGG	0.579028	5.3:-2581871	None:intergenic
TATATGGCGCAGAGCCCGGA+TGG	0.579495	5.3:+2582385	MS.gene024753:CDS
AGTTCAAGCATGCACCGGTG+TGG	0.580762	5.3:-2582204	None:intergenic
CAGATTTATACCGAATAGAT+CGG	0.585167	5.3:+2580837	MS.gene024753:CDS
TATTTGTGGGATGCATCAAA+TGG	0.585182	5.3:+2581559	MS.gene024753:CDS
CAGTAGCTAGCAAATTCCCT+TGG	0.590454	5.3:-2582141	None:intergenic
CTCACAACCGGAGGGATGGA+CGG	0.590810	5.3:+2581878	MS.gene024753:CDS
AACCGAGTTCAAGCATGCAC+CGG	0.591621	5.3:-2582209	None:intergenic
TGGAAACACAGTGTATTTGT+GGG	0.594599	5.3:+2581546	MS.gene024753:CDS
GGTGGTCATAGGCAGCGAGT+AGG	0.596714	5.3:+2581830	MS.gene024753:CDS
AATTTGCTAGCTACTGGTGG+AGG	0.597622	5.3:+2582148	MS.gene024753:CDS
CAGCAGCAGACGAAACATTG+AGG	0.598798	5.3:+2582425	MS.gene024753:CDS
GGGAATTTGCTAGCTACTGG+TGG	0.599431	5.3:+2582145	MS.gene024753:CDS
TATACCGAATAGATCGGCGA+TGG	0.599857	5.3:+2580843	MS.gene024753:CDS
ATCAAATCAACTGGAGTAGG+TGG	0.601482	5.3:-2581015	None:intergenic
AGCGAGTAGGGTCTTTGGCA+TGG	0.601597	5.3:+2581843	MS.gene024753:CDS
GGATTGAAATGGTCGGCTAA+CGG	0.603805	5.3:+2581977	MS.gene024753:CDS
ATGTTACTGCTTATCGAATG+CGG	0.611907	5.3:-2582528	None:intergenic
AAGATAAAACCATAAAGCCC+AGG	0.612129	5.3:+2581070	MS.gene024753:CDS
CCATAGCAGGGAACATACTT+GGG	0.613427	5.3:-2582242	None:intergenic
AGGGCCGTCTTCTTCTTCGA+CGG	0.614774	5.3:-2581602	None:intergenic
AAGTGCTATTGCAAGAACAT+TGG	0.617527	5.3:-2581524	None:intergenic
CAGTTGAGAACTTTGAAAGG+TGG	0.617886	5.3:+2581812	MS.gene024753:intron
GTCAACAATTAGCCAGTGGA+GGG	0.619435	5.3:+2581999	MS.gene024753:CDS
GGTCAACAATTAGCCAGTGG+AGG	0.633558	5.3:+2581998	MS.gene024753:CDS
TACCTCAATTTATTAGACTG+GGG	0.642053	5.3:+2581496	MS.gene024753:CDS
GCTATGGAACAAGAATGAAA+GGG	0.642631	5.3:+2582258	MS.gene024753:CDS
ACCACATACTCACAACCGGA+GGG	0.650856	5.3:+2581870	MS.gene024753:CDS
AGCCTGTGAAGCCACTGTGT+AGG	0.662766	5.3:-2582073	None:intergenic
ACACTACATGGTGACAGAAG+GGG	0.666148	5.3:+2580879	MS.gene024753:CDS
AACGGTCAACAATTAGCCAG+TGG	0.669539	5.3:+2581995	MS.gene024753:CDS
CTTACAGAGAAAGAGTTCCA+AGG	0.680127	5.3:+2580364	MS.gene024753:CDS
GTTACCGTCGAAGAAGAAGA+CGG	0.688864	5.3:+2581598	MS.gene024753:CDS
ACATTGTTGAAACATACAGA+GGG	0.688874	5.3:+2581936	MS.gene024753:CDS
AACCACATACTCACAACCGG+AGG	0.695066	5.3:+2581869	MS.gene024753:CDS
CACATTGTTGAAACATACAG+AGG	0.702329	5.3:+2581935	MS.gene024753:CDS
AGTTTGCGGATTGAAATGGT+CGG	0.710202	5.3:+2581970	MS.gene024753:CDS
TGAGGTAATAGTCATCCACA+AGG	0.712406	5.3:-2581480	None:intergenic
CTTCTAGTTCTCCTACACAG+TGG	0.755247	5.3:+2582062	MS.gene024753:CDS
GAGCCCGGATGGTTGCACAG+TGG	0.763260	5.3:+2582396	MS.gene024753:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTATTAATTCTTAGTTAAAA+GGG	+	chr5.3:2581232-2581251	MS.gene024753:intron	10.0%
!!	TTTATTAATTCTTAGTTAAA+AGG	+	chr5.3:2581231-2581250	MS.gene024753:intron	10.0%
!!!	AAGAATAAATTTTGAAATTT+TGG	+	chr5.3:2580514-2580533	MS.gene024753:intron	10.0%
!!!	AGAATAAATTTTGAAATTTT+GGG	+	chr5.3:2580515-2580534	MS.gene024753:intron	10.0%
!!!	TGTGAATAAATTTTAAAATT+GGG	+	chr5.3:2580551-2580570	MS.gene024753:intron	10.0%
!!!	TTGTGAATAAATTTTAAAAT+TGG	+	chr5.3:2580550-2580569	MS.gene024753:intron	10.0%
!!	AATAACAATGATAAAACATA+AGG	-	chr5.3:2581138-2581157	None:intergenic	15.0%
!!	ACTCATTCAAAATTTAAATT+TGG	-	chr5.3:2581184-2581203	None:intergenic	15.0%
!!!	AATAAATTTTGAAATTTTGG+GGG	+	chr5.3:2580517-2580536	MS.gene024753:intron	15.0%
!!!	ATGTTTTATCATTGTTATTT+TGG	+	chr5.3:2581139-2581158	MS.gene024753:intron	15.0%
!!!	GAATAAATTTTGAAATTTTG+GGG	+	chr5.3:2580516-2580535	MS.gene024753:intron	15.0%
!!!	GGATTTTAATTATCAAATTT+TGG	+	chr5.3:2580449-2580468	MS.gene024753:intron	15.0%
!!	ATTCAGATCTTTCAAAATTA+GGG	+	chr5.3:2580411-2580430	MS.gene024753:intron	20.0%
!!	CACAAAATTGCAAATTATTA+TGG	-	chr5.3:2581404-2581423	None:intergenic	20.0%
!!	TATTCAGATCTTTCAAAATT+AGG	+	chr5.3:2580410-2580429	MS.gene024753:intron	20.0%
!!!	ATTAGGGTTTTAATGTAAAT+TGG	+	chr5.3:2580427-2580446	MS.gene024753:intron	20.0%
!!!	ATTATCAAATTTTGGTGTTT+TGG	+	chr5.3:2580457-2580476	MS.gene024753:intron	20.0%
!!!	ATTTTTAATTTTCGAATTGG+GGG	+	chr5.3:2580574-2580593	MS.gene024753:intron	20.0%
!!!	GATTTTTAATTTTCGAATTG+GGG	+	chr5.3:2580573-2580592	MS.gene024753:intron	20.0%
!!!	GGATTTTTAATTTTCGAATT+GGG	+	chr5.3:2580572-2580591	MS.gene024753:intron	20.0%
!!!	TTAGGGTTTTAATGTAAATT+GGG	+	chr5.3:2580428-2580447	MS.gene024753:intron	20.0%
!	AAACCAAACAAGAATAATGT+TGG	-	chr5.3:2581789-2581808	None:intergenic	25.0%
!	ATGATAATCTACTTTACATC+TGG	+	chr5.3:2582023-2582042	MS.gene024753:CDS	25.0%
!	ATTACCTCAATTTATTAGAC+TGG	+	chr5.3:2581494-2581513	MS.gene024753:CDS	25.0%
!	TGATAATCTACTTTACATCT+GGG	+	chr5.3:2582024-2582043	MS.gene024753:CDS	25.0%
!	TTACCTCAATTTATTAGACT+GGG	+	chr5.3:2581495-2581514	MS.gene024753:CDS	25.0%
!	TTAGACTACTAATCCAATTA+TGG	-	chr5.3:2580483-2580502	None:intergenic	25.0%
!!	AAATGTTCTTACATTTTCCT+TGG	-	chr5.3:2580384-2580403	None:intergenic	25.0%
!!	AGGAATTTGATAGTTCATAA+AGG	-	chr5.3:2581757-2581776	None:intergenic	25.0%
!!	AGTTCATAAAGGTGTATTAA+AGG	-	chr5.3:2581746-2581765	None:intergenic	25.0%
!!!	AATGAGTTTTGAAAATCAAG+TGG	+	chr5.3:2581197-2581216	MS.gene024753:intron	25.0%
!!!	ATGAGTTTTGAAAATCAAGT+GGG	+	chr5.3:2581198-2581217	MS.gene024753:intron	25.0%
!!!	GGGATTTTTAATTTTCGAAT+TGG	+	chr5.3:2580571-2580590	MS.gene024753:intron	25.0%
!!!	TCATTGTTATTTTGGTTCTT+TGG	+	chr5.3:2581147-2581166	MS.gene024753:intron	25.0%
!!!	TTAGGTTTTTGAAATTCAAG+TGG	+	chr5.3:2581322-2581341	MS.gene024753:intron	25.0%
	ACATTGTTGAAACATACAGA+GGG	+	chr5.3:2581936-2581955	MS.gene024753:CDS	30.0%
	CACTTCAAGAGTTCTTTATA+TGG	+	chr5.3:2582369-2582388	MS.gene024753:CDS	30.0%
	CAGATTTATACCGAATAGAT+CGG	+	chr5.3:2580837-2580856	MS.gene024753:CDS	30.0%
	CTTAGTTAAAAGGGTTTGTA+TGG	+	chr5.3:2581241-2581260	MS.gene024753:intron	30.0%
	TACCTCAATTTATTAGACTG+GGG	+	chr5.3:2581496-2581515	MS.gene024753:CDS	30.0%
	TTGCCAACATTATTCTTGTT+TGG	+	chr5.3:2581783-2581802	MS.gene024753:intron	30.0%
!	ACTTTGGAAATATCCTTCAA+TGG	+	chr5.3:2582321-2582340	MS.gene024753:CDS	30.0%
!!	TCTTAGTTGAAAGTGTTGTA+TGG	+	chr5.3:2581365-2581384	MS.gene024753:intron	30.0%
	AAGATAAAACCATAAAGCCC+AGG	+	chr5.3:2581070-2581089	MS.gene024753:CDS	35.0%
	AATAATGTTGGCAAACTAGC+AGG	-	chr5.3:2581777-2581796	None:intergenic	35.0%
	AATGTTCTTGCAATAGCACT+TGG	+	chr5.3:2581526-2581545	MS.gene024753:CDS	35.0%
	AGATCATACATCTGCAGTAA+AGG	+	chr5.3:2582099-2582118	MS.gene024753:CDS	35.0%
	AGGTGTATTAAAGGTTAACC+AGG	-	chr5.3:2581737-2581756	None:intergenic	35.0%
	ATATCCTTCAATGGTGAAGA+TGG	+	chr5.3:2582330-2582349	MS.gene024753:CDS	35.0%
	CACATTGTTGAAACATACAG+AGG	+	chr5.3:2581935-2581954	MS.gene024753:CDS	35.0%
	CAGAGTCTTTAAACATGAAC+AGG	+	chr5.3:2580965-2580984	MS.gene024753:CDS	35.0%
	GCTATGGAACAAGAATGAAA+GGG	+	chr5.3:2582258-2582277	MS.gene024753:CDS	35.0%
	GTTCTTGAAAGCTAGAATTC+TGG	-	chr5.3:2580992-2581011	None:intergenic	35.0%
	TATTTGTGGGATGCATCAAA+TGG	+	chr5.3:2581559-2581578	MS.gene024753:CDS	35.0%
	TCATGAGGAATCAAATCAAC+TGG	-	chr5.3:2581027-2581046	None:intergenic	35.0%
	TGCTATGGAACAAGAATGAA+AGG	+	chr5.3:2582257-2582276	MS.gene024753:CDS	35.0%
	TGGAAACACAGTGTATTTGT+GGG	+	chr5.3:2581546-2581565	MS.gene024753:CDS	35.0%
	TTCATTCTTGTTCCATAGCA+GGG	-	chr5.3:2582257-2582276	None:intergenic	35.0%
	TTCCCCAGTCTAATAAATTG+AGG	-	chr5.3:2581501-2581520	None:intergenic	35.0%
	TTGGAAACACAGTGTATTTG+TGG	+	chr5.3:2581545-2581564	MS.gene024753:CDS	35.0%
	TTTCATTCTTGTTCCATAGC+AGG	-	chr5.3:2582258-2582277	None:intergenic	35.0%
	TTTCGATTATGCACACTACA+TGG	+	chr5.3:2580867-2580886	MS.gene024753:CDS	35.0%
!	AACAGGTTTTTACACTTACC+TGG	+	chr5.3:2581716-2581735	MS.gene024753:intron	35.0%
!	AAGAAGTTTGCGGATTGAAA+TGG	+	chr5.3:2581966-2581985	MS.gene024753:CDS	35.0%
!	AAGTGCTATTGCAAGAACAT+TGG	-	chr5.3:2581527-2581546	None:intergenic	35.0%
!	GATTAGTTGGCTATCTGATT+AGG	+	chr5.3:2581304-2581323	MS.gene024753:intron	35.0%
!!	AAAACGTGATTGGGTTTTGA+TGG	-	chr5.3:2580330-2580349	None:intergenic	35.0%
!!	TGTGCTAGTTGATGATTAGT+TGG	+	chr5.3:2581291-2581310	MS.gene024753:intron	35.0%
!!	TTGATTTGTTTGTTGCCAGT+TGG	+	chr5.3:2580813-2580832	MS.gene024753:intron	35.0%
!!	TTGTTTTTCAGACTTCTGAG+AGG	+	chr5.3:2581440-2581459	MS.gene024753:intron	35.0%
!!!	TTGAGGTTTTGGAATGCTTT+TGG	+	chr5.3:2582442-2582461	MS.gene024753:CDS	35.0%
!!!	TTTGGTGTTTTGGCCATAAT+TGG	+	chr5.3:2580467-2580486	MS.gene024753:intron	35.0%
	AAATCCATCGCCGATCTATT+CGG	-	chr5.3:2580850-2580869	None:intergenic	40.0%
	AACAACCACATACTCACAAC+CGG	+	chr5.3:2581866-2581885	MS.gene024753:CDS	40.0%
	AATAATTCGCCTGGGCTTTA+TGG	-	chr5.3:2581082-2581101	None:intergenic	40.0%
	AGATTTCAGTAACACACCTG+AGG	-	chr5.3:2581105-2581124	None:intergenic	40.0%
	ATCAAATCAACTGGAGTAGG+TGG	-	chr5.3:2581018-2581037	None:intergenic	40.0%
	CACAGAATCAGCTAACACTT+TGG	+	chr5.3:2582305-2582324	MS.gene024753:CDS	40.0%
	CTGAAGAGGAAAACGTGATT+GGG	-	chr5.3:2580339-2580358	None:intergenic	40.0%
	CTGCAGTTGAGAACTTTGAA+AGG	+	chr5.3:2581809-2581828	MS.gene024753:intron	40.0%
	CTTACAGAGAAAGAGTTCCA+AGG	+	chr5.3:2580364-2580383	MS.gene024753:CDS	40.0%
	GATGATGAAGTAAGCTCATG+AGG	-	chr5.3:2581042-2581061	None:intergenic	40.0%
	GGAATCAAATCAACTGGAGT+AGG	-	chr5.3:2581021-2581040	None:intergenic	40.0%
	GGATACAGCTTCAGATAAAC+AGG	+	chr5.3:2581699-2581718	MS.gene024753:CDS	40.0%
	TAATAGTCATCCACAAGGTC+TGG	-	chr5.3:2581478-2581497	None:intergenic	40.0%
	TAGATTATCATTCCCTCCAC+TGG	-	chr5.3:2582014-2582033	None:intergenic	40.0%
	TCGGTATAAATCTGTCCAAC+TGG	-	chr5.3:2580831-2580850	None:intergenic	40.0%
	TGAGGTAATAGTCATCCACA+AGG	-	chr5.3:2581483-2581502	None:intergenic	40.0%
!	AGTTTGCGGATTGAAATGGT+CGG	+	chr5.3:2581970-2581989	MS.gene024753:CDS	40.0%
!	CAGACGAAACATTGAGGTTT+TGG	+	chr5.3:2582431-2582450	MS.gene024753:CDS	40.0%
!	CAGTTGAGAACTTTGAAAGG+TGG	+	chr5.3:2581812-2581831	MS.gene024753:intron	40.0%
!	GAACTTTGAAAGGTGGTCAT+AGG	+	chr5.3:2581819-2581838	MS.gene024753:CDS	40.0%
!	GCGATCGAACAATCAAGTTT+TGG	+	chr5.3:2582176-2582195	MS.gene024753:CDS	40.0%
!	TTGGGGGTTTTCTTAGATTG+TGG	+	chr5.3:2580533-2580552	MS.gene024753:intron	40.0%
	AACGGTCAACAATTAGCCAG+TGG	+	chr5.3:2581995-2582014	MS.gene024753:CDS	45.0%
	AATGGCTCAGCTCTTGCTTT+CGG	-	chr5.3:2582496-2582515	None:intergenic	45.0%
	ACACTACATGGTGACAGAAG+GGG	+	chr5.3:2580879-2580898	MS.gene024753:CDS	45.0%
	ACCTGAGGAATAATTCGCCT+GGG	-	chr5.3:2581090-2581109	None:intergenic	45.0%
	ATGCGGCTCACATGTGAAAA+TGG	-	chr5.3:2582514-2582533	None:intergenic	45.0%
	CACACTACATGGTGACAGAA+GGG	+	chr5.3:2580878-2580897	MS.gene024753:CDS	45.0%
	CAGTAGCTAGCAAATTCCCT+TGG	-	chr5.3:2582144-2582163	None:intergenic	45.0%
	CCATAGCAGGGAACATACTT+GGG	-	chr5.3:2582245-2582264	None:intergenic	45.0%
	CCCAACTCACAGATGTGATA+GGG	-	chr5.3:2581624-2581643	None:intergenic	45.0%
	CCCTATCACATCTGTGAGTT+GGG	+	chr5.3:2581621-2581640	MS.gene024753:CDS	45.0%
	CCTGAAGAGGAAAACGTGAT+TGG	-	chr5.3:2580340-2580359	None:intergenic	45.0%
	CTCTGTAAGTGTTCCTGAAG+AGG	-	chr5.3:2580353-2580372	None:intergenic	45.0%
	GCTAGCAAATTCCCTTGGAA+TGG	-	chr5.3:2582139-2582158	None:intergenic	45.0%
	GGATTGAAATGGTCGGCTAA+CGG	+	chr5.3:2581977-2581996	MS.gene024753:CDS	45.0%
	GGGCATGAACAAGAAGTTTG+CGG	+	chr5.3:2581956-2581975	MS.gene024753:CDS	45.0%
	GTCAACAATTAGCCAGTGGA+GGG	+	chr5.3:2581999-2582018	MS.gene024753:CDS	45.0%
	GTTACCGTCGAAGAAGAAGA+CGG	+	chr5.3:2581598-2581617	MS.gene024753:CDS	45.0%
	TATACCGAATAGATCGGCGA+TGG	+	chr5.3:2580843-2580862	MS.gene024753:CDS	45.0%
	TCCATAGCAGGGAACATACT+TGG	-	chr5.3:2582246-2582265	None:intergenic	45.0%
	TCTGCCATCTTCACCATTGA+AGG	-	chr5.3:2582337-2582356	None:intergenic	45.0%
!	AATTTTACCGTCCATCCCTC+CGG	-	chr5.3:2581888-2581907	None:intergenic	45.0%
!	CCAATCACGTTTTCCTCTTC+AGG	+	chr5.3:2580337-2580356	MS.gene024753:CDS	45.0%
!	CTACTTTACATCTGGGACAG+AGG	+	chr5.3:2582031-2582050	MS.gene024753:CDS	45.0%
!	GCAGCTTTTGCTACTACTTC+TGG	-	chr5.3:2582472-2582491	None:intergenic	45.0%
!	TCAGACTTCTGAGAGGACTT+TGG	+	chr5.3:2581447-2581466	MS.gene024753:intron	45.0%
!!	AATTTGCTAGCTACTGGTGG+AGG	+	chr5.3:2582148-2582167	MS.gene024753:CDS	45.0%
!!	ACAACTCTGAAGTGCAGCTA+TGG	+	chr5.3:2581677-2581696	MS.gene024753:CDS	45.0%
!!	ACGTGATTGGGTTTTGATGG+CGG	-	chr5.3:2580327-2580346	None:intergenic	45.0%
!!	CAACTCTGAAGTGCAGCTAT+GGG	+	chr5.3:2581678-2581697	MS.gene024753:CDS	45.0%
!!	CAAGGGAATTTGCTAGCTAC+TGG	+	chr5.3:2582142-2582161	MS.gene024753:CDS	45.0%
!!	CTTCTAGTTCTCCTACACAG+TGG	+	chr5.3:2582062-2582081	MS.gene024753:CDS	45.0%
!!	TTGCTTGGTGTCCATTCCAA+GGG	+	chr5.3:2582125-2582144	MS.gene024753:CDS	45.0%
!!!	AAGTTTTGGAACACCCACAC+CGG	+	chr5.3:2582190-2582209	MS.gene024753:CDS	45.0%
	AACCACATACTCACAACCGG+AGG	+	chr5.3:2581869-2581888	MS.gene024753:CDS	50.0%
	AACCGAGTTCAAGCATGCAC+CGG	-	chr5.3:2582212-2582231	None:intergenic	50.0%
	ACCACATACTCACAACCGGA+GGG	+	chr5.3:2581870-2581889	MS.gene024753:CDS	50.0%
	AGGGAGTTACTCAGCTCTCA+TGG	+	chr5.3:2582277-2582296	MS.gene024753:CDS	50.0%
	ATGCCAATATGACGCCCATC+AGG	-	chr5.3:2581649-2581668	None:intergenic	50.0%
	ATGGTGACAGAAGGGGCTAA+AGG	+	chr5.3:2580886-2580905	MS.gene024753:CDS	50.0%
	CACCTGAGGAATAATTCGCC+TGG	-	chr5.3:2581091-2581110	None:intergenic	50.0%
	CAGCAGCAGACGAAACATTG+AGG	+	chr5.3:2582425-2582444	MS.gene024753:CDS	50.0%
	CCCAAGTATGTTCCCTGCTA+TGG	+	chr5.3:2582242-2582261	MS.gene024753:CDS	50.0%
	CTGCAGTAAAGGCTCTTGCT+TGG	+	chr5.3:2582110-2582129	MS.gene024753:CDS	50.0%
	GACAGAAGGGGCTAAAGGTA+AGG	+	chr5.3:2580891-2580910	MS.gene024753:CDS	50.0%
	GATGGGCGTCATATTGGCAT+TGG	+	chr5.3:2581649-2581668	MS.gene024753:CDS	50.0%
	GCACACTACATGGTGACAGA+AGG	+	chr5.3:2580877-2580896	MS.gene024753:CDS	50.0%
	GCCCAACTCACAGATGTGAT+AGG	-	chr5.3:2581625-2581644	None:intergenic	50.0%
	GCCCTATCACATCTGTGAGT+TGG	+	chr5.3:2581620-2581639	MS.gene024753:CDS	50.0%
	GCTTGAACTCGGTTGACACT+GGG	+	chr5.3:2582218-2582237	MS.gene024753:CDS	50.0%
	GGTCAACAATTAGCCAGTGG+AGG	+	chr5.3:2581998-2582017	MS.gene024753:CDS	50.0%
	GTGAAGATGGCAGAGCTCAA+TGG	+	chr5.3:2582343-2582362	MS.gene024753:CDS	50.0%
	TAGCTTCCTGTACGCTTCTC+TGG	-	chr5.3:2580941-2580960	None:intergenic	50.0%
	TCTTTATATGGCGCAGAGCC+CGG	+	chr5.3:2582381-2582400	MS.gene024753:CDS	50.0%
	TGCTTGAACTCGGTTGACAC+TGG	+	chr5.3:2582217-2582236	MS.gene024753:CDS	50.0%
	TTCCTGTACGCTTCTCTGGA+AGG	-	chr5.3:2580937-2580956	None:intergenic	50.0%
!	TCCTGTACGCTTCTCTGGAA+GGG	-	chr5.3:2580936-2580955	None:intergenic	50.0%
!!	CGTGATTGGGTTTTGATGGC+GGG	-	chr5.3:2580326-2580345	None:intergenic	50.0%
!!	CTTGCTTGGTGTCCATTCCA+AGG	+	chr5.3:2582124-2582143	MS.gene024753:CDS	50.0%
!!	GGGAATTTGCTAGCTACTGG+TGG	+	chr5.3:2582145-2582164	MS.gene024753:CDS	50.0%
	AGCCTGTGAAGCCACTGTGT+AGG	-	chr5.3:2582076-2582095	None:intergenic	55.0%
	AGCGAGTAGGGTCTTTGGCA+TGG	+	chr5.3:2581843-2581862	MS.gene024753:CDS	55.0%
	AGGGCCGTCTTCTTCTTCGA+CGG	-	chr5.3:2581605-2581624	None:intergenic	55.0%
	CACCGGTGCATGCTTGAACT+CGG	+	chr5.3:2582207-2582226	MS.gene024753:CDS	55.0%
	CATACTCACAACCGGAGGGA+TGG	+	chr5.3:2581874-2581893	MS.gene024753:CDS	55.0%
	CTCCTACACAGTGGCTTCAC+AGG	+	chr5.3:2582071-2582090	MS.gene024753:CDS	55.0%
	GCCCAGGCGAATTATTCCTC+AGG	+	chr5.3:2581086-2581105	MS.gene024753:CDS	55.0%
	GTGGTCATAGGCAGCGAGTA+GGG	+	chr5.3:2581831-2581850	MS.gene024753:CDS	55.0%
	GTTGCCACTGTGCAACCATC+CGG	-	chr5.3:2582403-2582422	None:intergenic	55.0%
	TAGGCAGCGAGTAGGGTCTT+TGG	+	chr5.3:2581838-2581857	MS.gene024753:CDS	55.0%
	TCCCTCCGGTTGTGAGTATG+TGG	-	chr5.3:2581874-2581893	None:intergenic	55.0%
	TTGCCACTGTGCAACCATCC+GGG	-	chr5.3:2582402-2582421	None:intergenic	55.0%
	TTTGGACGCTCCAGACCTTG+TGG	+	chr5.3:2581465-2581484	MS.gene024753:CDS	55.0%
!	AGTTCAAGCATGCACCGGTG+TGG	-	chr5.3:2582207-2582226	None:intergenic	55.0%
!	CCTGTACGCTTCTCTGGAAG+GGG	-	chr5.3:2580935-2580954	None:intergenic	55.0%
!	CTGTGAGTTGGGCTCCTGAT+GGG	+	chr5.3:2581632-2581651	MS.gene024753:CDS	55.0%
!	GCTCCTGATGGGCGTCATAT+TGG	+	chr5.3:2581643-2581662	MS.gene024753:CDS	55.0%
!	GTTCAAGCATGCACCGGTGT+GGG	-	chr5.3:2582206-2582225	None:intergenic	55.0%
!	TCTGTGAGTTGGGCTCCTGA+TGG	+	chr5.3:2581631-2581650	MS.gene024753:CDS	55.0%
	CTAGCTACTGGTGGAGGCAG+TGG	+	chr5.3:2582154-2582173	MS.gene024753:CDS	60.0%
	CTCACAACCGGAGGGATGGA+CGG	+	chr5.3:2581878-2581897	MS.gene024753:CDS	60.0%
	GGTGGTCATAGGCAGCGAGT+AGG	+	chr5.3:2581830-2581849	MS.gene024753:CDS	60.0%
	TATATGGCGCAGAGCCCGGA+TGG	+	chr5.3:2582385-2582404	MS.gene024753:CDS	60.0%
!	CCCCTTCCAGAGAAGCGTAC+AGG	+	chr5.3:2580932-2580951	MS.gene024753:CDS	60.0%
!	CTGGAAGGGGAGCACACTTC+TGG	-	chr5.3:2580922-2580941	None:intergenic	60.0%
	GAGCCCGGATGGTTGCACAG+TGG	+	chr5.3:2582396-2582415	MS.gene024753:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	2580299	2582540	2580299	ID=MS.gene024753
chr5.3	mRNA	2580299	2582540	2580299	ID=MS.gene024753.t1;Parent=MS.gene024753
chr5.3	exon	2580299	2580391	2580299	ID=MS.gene024753.t1.exon1;Parent=MS.gene024753.t1
chr5.3	CDS	2580299	2580391	2580299	ID=cds.MS.gene024753.t1;Parent=MS.gene024753.t1
chr5.3	exon	2580832	2581107	2580832	ID=MS.gene024753.t1.exon2;Parent=MS.gene024753.t1
chr5.3	CDS	2580832	2581107	2580832	ID=cds.MS.gene024753.t1;Parent=MS.gene024753.t1
chr5.3	exon	2581451	2581720	2581451	ID=MS.gene024753.t1.exon3;Parent=MS.gene024753.t1
chr5.3	CDS	2581451	2581720	2581451	ID=cds.MS.gene024753.t1;Parent=MS.gene024753.t1
chr5.3	exon	2581815	2582540	2581815	ID=MS.gene024753.t1.exon4;Parent=MS.gene024753.t1
chr5.3	CDS	2581815	2582540	2581815	ID=cds.MS.gene024753.t1;Parent=MS.gene024753.t1

Gene Sequence

>MS.gene024753

ATGGCTTCAGGTTCTTTGAGTTCTCCCGCCATCAAAACCCAATCACGTTTTCCTCTTCAGGAACACTTACAGAGAAAGAGTTCCAAGGAAAATGTAAGAACATTTTTCAATTATTCAGATCTTTCAAAATTAGGGTTTTAATGTAAATTGGGATTTTAATTATCAAATTTTGGTGTTTTGGCCATAATTGGATTAGTAGTCTAAAAACTGTGATAAAGAATAAATTTTGAAATTTTGGGGGTTTTCTTAGATTGTGAATAAATTTTAAAATTGGGATTTTTAATTTTCGAATTGGGGGTTTTCTTAGATTGTGGATTAATTTTGAAAATATGTTAAAAAAGAATGAATTTTGACATTATGTCATGAAAAAGAATGAATTTTGACATTATGTCATGAAAAAGAATGAATTTTGAAATTATGCTAAACTTTTTATTGACTGAGTTGAAATTATGATATGATAAGTTGATAATATTCTGAATTTGTTTATGAAAATCAAGTATTGATTCTTTATTGTTTGATTTGTTTGTTGCCAGTTGGACAGATTTATACCGAATAGATCGGCGATGGATTTCGATTATGCACACTACATGGTGACAGAAGGGGCTAAAGGTAAGGAAAATCCAGAAGTGTGCTCCCCTTCCAGAGAAGCGTACAGGAAGCTACTTGCAGAGTCTTTAAACATGAACAGGACCAGAATTCTAGCTTTCAAGAACAAGCCACCTACTCCAGTTGATTTGATTCCTCATGAGCTTACTTCATCATCTCTTCAACAAGATAAAACCATAAAGCCCAGGCGAATTATTCCTCAGGTGTGTTACTGAAATCTGATTGATATTCCTTATGTTTTATCATTGTTATTTTGGTTCTTTGGTTGCATGAACTCCAAATTTAAATTTTGAATGAGTTTTGAAAATCAAGTGGGTCTGAGTTGTTTTATTAATTCTTAGTTAAAAGGGTTTGTATGGTGAATGAAATTCAATATTTTGCAATTTTGTGCTAGTTGATGATTAGTTGGCTATCTGATTAGGTTTTTGAAATTCAAGTGGATCTGAGTTGTTTTCTTAATTCTTAGTTGAAAGTGTTGTATGGTATAAACTGTAAACCATAATAATTTGCAATTTTGTGCTAATTGATTGATATGTTGTTTTTCAGACTTCTGAGAGGACTTTGGACGCTCCAGACCTTGTGGATGACTATTACCTCAATTTATTAGACTGGGGAAGCGCCAATGTTCTTGCAATAGCACTTGGAAACACAGTGTATTTGTGGGATGCATCAAATGGTTCTACTTCAGAACTTGTTACCGTCGAAGAAGAAGACGGCCCTATCACATCTGTGAGTTGGGCTCCTGATGGGCGTCATATTGGCATTGGTTTGAACAACTCTGAAGTGCAGCTATGGGATACAGCTTCAGATAAACAGGTTTTTACACTTACCTGGTTAACCTTTAATACACCTTTATGAACTATCAAATTCCTGCTAGTTTGCCAACATTATTCTTGTTTGGTTTCTGCAGTTGAGAACTTTGAAAGGTGGTCATAGGCAGCGAGTAGGGTCTTTGGCATGGAACAACCACATACTCACAACCGGAGGGATGGACGGTAAAATTATCAATAATGATGTAAGAATTAGAGCACACATTGTTGAAACATACAGAGGGCATGAACAAGAAGTTTGCGGATTGAAATGGTCGGCTAACGGTCAACAATTAGCCAGTGGAGGGAATGATAATCTACTTTACATCTGGGACAGAGGTACATCAACTTCTAGTTCTCCTACACAGTGGCTTCACAGGCTTGAAGATCATACATCTGCAGTAAAGGCTCTTGCTTGGTGTCCATTCCAAGGGAATTTGCTAGCTACTGGTGGAGGCAGTGGCGATCGAACAATCAAGTTTTGGAACACCCACACCGGTGCATGCTTGAACTCGGTTGACACTGGGTCCCAAGTATGTTCCCTGCTATGGAACAAGAATGAAAGGGAGTTACTCAGCTCTCATGGTTTTACACAGAATCAGCTAACACTTTGGAAATATCCTTCAATGGTGAAGATGGCAGAGCTCAATGGTCACACTTCAAGAGTTCTTTATATGGCGCAGAGCCCGGATGGTTGCACAGTGGCAACAGCAGCAGCAGACGAAACATTGAGGTTTTGGAATGCTTTTGGCACTCCAGAAGTAGTAGCAAAAGCTGCACCGAAAGCAAGAGCTGAGCCATTTTCACATGTGAGCCGCATTCGATAA

Protein sequence

>MS.gene024753.t1

MASGSLSSPAIKTQSRFPLQEHLQRKSSKENLDRFIPNRSAMDFDYAHYMVTEGAKGKENPEVCSPSREAYRKLLAESLNMNRTRILAFKNKPPTPVDLIPHELTSSSLQQDKTIKPRRIIPQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWDASNGSTSELVTVEEEDGPITSVSWAPDGRHIGIGLNNSEVQLWDTASDKQLRTLKGGHRQRVGSLAWNNHILTTGGMDGKIINNDVRIRAHIVETYRGHEQEVCGLKWSANGQQLASGGNDNLLYIWDRGTSTSSSPTQWLHRLEDHTSAVKALAWCPFQGNLLATGGGSGDRTIKFWNTHTGACLNSVDTGSQVCSLLWNKNERELLSSHGFTQNQLTLWKYPSMVKMAELNGHTSRVLYMAQSPDGCTVATAAADETLRFWNAFGTPEVVAKAAPKARAEPFSHVSRIR