AlfalfaGEDB | MS.gene30174

M. sativa cultivar XinJiangDaYe / MS.gene30174

KEGG: K03363
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00400.35 PF12894.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30174.t1	XP_003600092.1	93.7	459	23	2	1	453	1	459	5.80E-254	886.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30174.t1	Q9SZA4	76.4	453	98	4	7	453	8	457	6.9e-203	708.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30174.t1	G7IY01	93.7	459	23	2	1	453	1	459	4.2e-254	886.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene30174	MS.gene033401	PPI
MS.gene75059	MS.gene30174	PPI
MS.gene23811	MS.gene30174	PPI
MS.gene34712	MS.gene30174	PPI
MS.gene92674	MS.gene30174	PPI
MS.gene51335	MS.gene30174	PPI
MS.gene82021	MS.gene30174	PPI
MS.gene30174	MS.gene06177	PPI
MS.gene30174	MS.gene51278	PPI
MS.gene027441	MS.gene30174	PPI
MS.gene81663	MS.gene30174	PPI
MS.gene068249	MS.gene30174	PPI
MS.gene81660	MS.gene30174	PPI
MS.gene066046	MS.gene30174	PPI
MS.gene30174	MS.gene014321	PPI
MS.gene41291	MS.gene30174	PPI
MS.gene30174	MS.gene064862	PPI
MS.gene30174	MS.gene23881	PPI
MS.gene27223	MS.gene30174	PPI
MS.gene66342	MS.gene30174	PPI
MS.gene038519	MS.gene30174	PPI
MS.gene050272	MS.gene30174	PPI
MS.gene06176	MS.gene30174	PPI
MS.gene030294	MS.gene30174	PPI
MS.gene014419	MS.gene30174	PPI
MS.gene30174	MS.gene30177	PPI
MS.gene30174	MS.gene84466	PPI
MS.gene91851	MS.gene30174	PPI
MS.gene29768	MS.gene30174	PPI
MS.gene039682	MS.gene30174	PPI
MS.gene043742	MS.gene30174	PPI
MS.gene022502	MS.gene30174	PPI
MS.gene85534	MS.gene30174	PPI
MS.gene066467	MS.gene30174	PPI
MS.gene82020	MS.gene30174	PPI
MS.gene21734	MS.gene30174	PPI
MS.gene66343	MS.gene30174	PPI
MS.gene33377	MS.gene30174	PPI
MS.gene30174	MS.gene027072	PPI
MS.gene71204	MS.gene30174	PPI
MS.gene30174	MS.gene65463	PPI
MS.gene064035	MS.gene30174	PPI
MS.gene77885	MS.gene30174	PPI
MS.gene30174	MS.gene87218	PPI
MS.gene26672	MS.gene30174	PPI
MS.gene36842	MS.gene30174	PPI
MS.gene99554	MS.gene30174	PPI
MS.gene90626	MS.gene30174	PPI
MS.gene050456	MS.gene30174	PPI
MS.gene058128	MS.gene30174	PPI
MS.gene99588	MS.gene30174	PPI
MS.gene027075	MS.gene30174	PPI
MS.gene011448	MS.gene30174	PPI
MS.gene30174	MS.gene37226	PPI
MS.gene066501	MS.gene30174	PPI
MS.gene068273	MS.gene30174	PPI
MS.gene63085	MS.gene30174	PPI
MS.gene55883	MS.gene30174	PPI
MS.gene056455	MS.gene30174	PPI
MS.gene30174	MS.gene99973	PPI
MS.gene89986	MS.gene30174	PPI
MS.gene26019	MS.gene30174	PPI
MS.gene064762	MS.gene30174	PPI
MS.gene30174	MS.gene008833	PPI
MS.gene30174	MS.gene064435	PPI
MS.gene052956	MS.gene30174	PPI
MS.gene049022	MS.gene30174	PPI
MS.gene91775	MS.gene30174	PPI
MS.gene35613	MS.gene30174	PPI
MS.gene30177	MS.gene30174	PPI
MS.gene98540	MS.gene30174	PPI
MS.gene69965	MS.gene30174	PPI
MS.gene066500	MS.gene30174	PPI
MS.gene30174	MS.gene48655	PPI
MS.gene21888	MS.gene30174	PPI
MS.gene067321	MS.gene30174	PPI
MS.gene82740	MS.gene30174	PPI
MS.gene30174	MS.gene32723	PPI
MS.gene049021	MS.gene30174	PPI
MS.gene30172	MS.gene30174	PPI
MS.gene067322	MS.gene30174	PPI
MS.gene045937	MS.gene30174	PPI
MS.gene23852	MS.gene30174	PPI
MS.gene69966	MS.gene30174	PPI
MS.gene97943	MS.gene30174	PPI
MS.gene30174	MS.gene037184	PPI
MS.gene027071	MS.gene30174	PPI
MS.gene97379	MS.gene30174	PPI
MS.gene67157	MS.gene30174	PPI
MS.gene69396	MS.gene30174	PPI
MS.gene42253	MS.gene30174	PPI
MS.gene30174	MS.gene067705	PPI
MS.gene012203	MS.gene30174	PPI
MS.gene022109	MS.gene30174	PPI
MS.gene34651	MS.gene30174	PPI
MS.gene25244	MS.gene30174	PPI
MS.gene014931	MS.gene30174	PPI
MS.gene010910	MS.gene30174	PPI
MS.gene052188	MS.gene30174	PPI
MS.gene025229	MS.gene30174	PPI
MS.gene045133	MS.gene30174	PPI
MS.gene063222	MS.gene30174	PPI
MS.gene25515	MS.gene30174	PPI
MS.gene060495	MS.gene30174	PPI
MS.gene07706	MS.gene30174	PPI
MS.gene027074	MS.gene30174	PPI
MS.gene069995	MS.gene30174	PPI
MS.gene30174	MS.gene26021	PPI
MS.gene062662	MS.gene30174	PPI
MS.gene69687	MS.gene30174	PPI
MS.gene30174	MS.gene053363	PPI
MS.gene51258	MS.gene30174	PPI
MS.gene23810	MS.gene30174	PPI
MS.gene46611	MS.gene30174	PPI
MS.gene30174	MS.gene75493	PPI
MS.gene024753	MS.gene30174	PPI
MS.gene81658	MS.gene30174	PPI
MS.gene30174	MS.gene32726	PPI
MS.gene84465	MS.gene30174	PPI
MS.gene30174	MS.gene71122	PPI
MS.gene78281	MS.gene30174	PPI
MS.gene55882	MS.gene30174	PPI
MS.gene037950	MS.gene30174	PPI
MS.gene70008	MS.gene30174	PPI
MS.gene060496	MS.gene30174	PPI
MS.gene042783	MS.gene30174	PPI
MS.gene64441	MS.gene30174	PPI
MS.gene24806	MS.gene30174	PPI
MS.gene071113	MS.gene30174	PPI
MS.gene004844	MS.gene30174	PPI
MS.gene52355	MS.gene30174	PPI
MS.gene30176	MS.gene30174	PPI
MS.gene30174	MS.gene21888	PPI
MS.gene24951	MS.gene30174	PPI
MS.gene066464	MS.gene30174	PPI
MS.gene20161	MS.gene30174	PPI
MS.gene30174	MS.gene62819	PPI
MS.gene51262	MS.gene30174	PPI
MS.gene050270	MS.gene30174	PPI
MS.gene20160	MS.gene30174	PPI
MS.gene24922	MS.gene30174	PPI
MS.gene027073	MS.gene30174	PPI
MS.gene33406	MS.gene30174	PPI
MS.gene30174	MS.gene65775	PPI
MS.gene30174	MS.gene80163	PPI
MS.gene010912	MS.gene30174	PPI
MS.gene30174	MS.gene29842	PPI
MS.gene050457	MS.gene30174	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30174.t1	MTR_3g051690	93.682	459	23	2	1	453	1	459	0.0	896
MS.gene30174.t1	MTR_5g008010	89.520	458	39	4	1	453	1	454	0.0	849
MS.gene30174.t1	MTR_7g106870	72.727	451	110	7	12	453	11	457	0.0	682
MS.gene30174.t1	MTR_7g451830	64.877	447	131	9	15	453	3	431	0.0	566
MS.gene30174.t1	MTR_7g047640	64.877	447	131	9	15	453	3	431	0.0	566
MS.gene30174.t1	MTR_7g057540	63.616	448	132	9	14	453	2	426	0.0	546
MS.gene30174.t1	MTR_7g055590	63.535	447	132	9	14	452	2	425	0.0	543
MS.gene30174.t1	MTR_7g053100	59.956	452	151	9	15	453	2	436	0.0	521
MS.gene30174.t1	MTR_7g034625	70.755	318	86	2	128	438	13	330	1.75e-167	474
MS.gene30174.t1	MTR_2g100540	50.114	439	164	12	25	453	4	397	3.06e-128	377
MS.gene30174.t1	MTR_2g100460	57.143	343	133	5	111	451	52	382	7.58e-127	373
MS.gene30174.t1	MTR_2g060710	44.282	411	207	8	33	429	26	428	8.24e-119	355
MS.gene30174.t1	MTR_3g067940	43.571	420	215	7	33	440	57	466	7.17e-113	340
MS.gene30174.t1	MTR_4g102510	41.527	419	213	6	33	432	51	456	3.70e-106	323
MS.gene30174.t1	MTR_4g415070	58.621	58	24	0	378	435	57	114	1.46e-16	80.9
MS.gene30174.t1	MTR_2g012630	26.695	236	159	9	144	373	18	245	4.74e-16	81.3
MS.gene30174.t1	MTR_2g012630	26.695	236	159	9	144	373	18	245	5.71e-16	81.3
MS.gene30174.t1	MTR_6g004040	27.039	233	156	9	144	370	19	243	4.17e-15	78.2
MS.gene30174.t1	MTR_1g054300	26.263	297	161	10	178	427	114	399	7.39e-15	77.0
MS.gene30174.t1	MTR_1g054300	26.119	268	170	11	165	425	235	481	2.30e-14	75.5
MS.gene30174.t1	MTR_5g016370	22.984	248	140	4	184	425	348	550	8.37e-14	73.9
MS.gene30174.t1	MTR_3g106080	23.494	332	212	10	135	434	302	623	5.30e-12	68.6
MS.gene30174.t1	MTR_4g095058	25.000	252	169	10	186	426	292	534	7.86e-12	67.8
MS.gene30174.t1	MTR_5g016350	22.581	248	153	5	178	425	330	538	8.20e-12	67.8
MS.gene30174.t1	MTR_4g095058	25.000	252	169	10	186	426	290	532	9.11e-12	67.4
MS.gene30174.t1	MTR_5g016350	22.490	249	154	5	178	426	266	475	1.11e-11	67.0
MS.gene30174.t1	MTR_7g086360	23.415	205	150	4	224	426	53	252	1.63e-11	65.9
MS.gene30174.t1	MTR_1g049000	39.362	94	47	3	204	288	8	100	9.75e-11	61.2
MS.gene30174.t1	MTR_4g119620	22.581	248	177	7	186	425	81	321	9.89e-11	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30174.t1	AT4G33270	76.380	453	98	5	7	453	8	457	0.0	715
MS.gene30174.t1	AT4G33260	76.802	444	94	5	16	453	7	447	0.0	688
MS.gene30174.t1	AT4G33260	74.473	427	100	5	16	436	7	430	0.0	653
MS.gene30174.t1	AT5G27570	68.132	455	129	7	7	453	4	450	0.0	618
MS.gene30174.t1	AT5G27080	67.426	439	126	7	23	453	13	442	0.0	594
MS.gene30174.t1	AT5G26900	66.893	441	129	7	21	453	13	444	0.0	586
MS.gene30174.t1	AT5G27945	61.995	371	121	5	99	453	62	428	2.80e-160	460
MS.gene30174.t1	AT4G22910	42.512	414	213	7	33	432	62	464	3.06e-109	332
MS.gene30174.t1	AT4G11920	41.647	425	214	8	33	438	53	462	1.91e-107	327
MS.gene30174.t1	AT5G13840	50.492	305	148	3	131	435	164	465	2.60e-107	327
MS.gene30174.t1	AT5G13840	50.492	305	148	3	131	435	164	465	2.60e-107	327
MS.gene30174.t1	AT5G08390	29.060	234	136	10	140	370	37	243	3.29e-16	82.0
MS.gene30174.t1	AT1G11160	28.641	206	134	8	144	344	18	215	3.96e-16	81.6
MS.gene30174.t1	AT1G11160	28.641	206	134	8	144	344	18	215	4.04e-16	81.6
MS.gene30174.t1	AT1G11160	28.641	206	134	8	144	344	18	215	4.57e-16	81.6
MS.gene30174.t1	AT1G61210	27.230	213	126	7	140	350	36	221	5.56e-15	78.2
MS.gene30174.t1	AT1G61210	27.230	213	126	7	140	350	36	221	5.71e-15	78.2
MS.gene30174.t1	AT1G11160	30.247	162	105	6	185	344	18	173	5.78e-15	78.2
MS.gene30174.t1	AT1G61210	27.230	213	126	7	140	350	36	221	6.38e-15	77.8
MS.gene30174.t1	AT5G52820	28.287	251	150	13	186	427	244	473	3.03e-14	75.1
MS.gene30174.t1	AT5G23430	28.169	213	124	8	140	350	37	222	5.80e-14	74.7
MS.gene30174.t1	AT5G23430	28.169	213	124	8	140	350	37	222	5.80e-14	74.7
MS.gene30174.t1	AT5G23430	28.169	213	124	8	140	350	37	222	6.11e-14	74.7
MS.gene30174.t1	AT5G25150	25.449	334	210	12	135	440	305	627	3.56e-13	72.0
MS.gene30174.t1	AT5G25150	25.449	334	210	12	135	440	305	627	4.62e-13	71.6
MS.gene30174.t1	AT2G32700	25.203	246	164	11	186	425	574	805	3.46e-12	69.3
MS.gene30174.t1	AT2G32700	25.203	246	164	11	186	425	555	786	3.83e-12	68.9
MS.gene30174.t1	AT2G32700	25.203	246	164	11	186	425	555	786	3.83e-12	68.9
MS.gene30174.t1	AT2G32700	25.203	246	164	11	186	425	555	786	3.83e-12	68.9
MS.gene30174.t1	AT2G32700	25.203	246	164	11	186	425	555	786	3.83e-12	68.9
MS.gene30174.t1	AT2G32700	25.203	246	164	11	186	425	555	786	3.83e-12	68.9
MS.gene30174.t1	AT2G32700	25.203	246	164	11	186	425	553	784	4.07e-12	68.9
MS.gene30174.t1	AT3G49660	23.600	250	177	8	182	425	71	312	8.32e-12	66.6
MS.gene30174.t1	AT2G41500	25.203	246	134	8	186	429	342	539	8.70e-12	67.8
MS.gene30174.t1	AT2G41500	25.203	246	134	8	186	429	342	539	8.70e-12	67.8
MS.gene30174.t1	AT5G13480	31.319	182	115	7	162	340	241	415	8.90e-12	67.8
MS.gene30174.t1	AT5G13480	27.391	230	148	10	186	408	137	354	1.89e-11	66.6
MS.gene30174.t1	AT5G13480	31.319	182	115	7	162	340	218	392	1.02e-11	67.4
MS.gene30174.t1	AT5G13480	27.391	230	148	10	186	408	114	331	2.42e-11	66.2
MS.gene30174.t1	AT5G13480	31.319	182	115	7	162	340	218	392	1.15e-11	67.4
MS.gene30174.t1	AT5G13480	27.391	230	148	10	186	408	114	331	2.85e-11	66.2
MS.gene30174.t1	AT5G67320	24.016	254	151	8	175	426	356	569	1.19e-11	67.4
MS.gene30174.t1	AT5G13480	31.319	182	115	7	162	340	230	404	1.36e-11	67.0
MS.gene30174.t1	AT5G13480	27.391	230	148	10	186	408	126	343	3.38e-11	65.9
MS.gene30174.t1	AT2G33340	26.042	192	134	5	238	426	236	422	4.64e-11	65.1
MS.gene30174.t1	AT2G33340	26.042	192	134	5	238	426	236	422	4.77e-11	65.1
MS.gene30174.t1	AT2G33340	26.042	192	134	5	238	426	236	422	4.77e-11	65.1
MS.gene30174.t1	AT1G04510	25.116	215	152	5	238	448	236	445	5.38e-11	65.1
MS.gene30174.t1	AT1G04510	25.116	215	152	5	238	448	236	445	5.67e-11	65.1
MS.gene30174.t1	AT4G02730	26.852	216	145	9	216	426	34	241	9.29e-11	63.5
MS.gene30174.t1	AT5G56130	23.786	206	113	5	263	426	16	219	9.62e-11	63.2

Find 137 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTACCTCAATTTACTGGATT+TGG	0.174186	3.3:+43058993	MS.gene30174:CDS
CGGATGAAACCTTGAGGTTT+TGG	0.196593	3.3:+43059928	MS.gene30174:CDS
TGACTATTACCTCAATTTAC+TGG	0.257532	3.3:+43058987	MS.gene30174:CDS
CTCCTTCCAGAGAAGCTTAC+AGG	0.301895	3.3:+43058414	MS.gene30174:CDS
TCAGACTTCTGAGAGGATAT+TGG	0.304579	3.3:+43058945	MS.gene30174:intron
TACCTCAATTTACTGGATTT+GGG	0.305684	3.3:+43058994	MS.gene30174:CDS
TCTCATTCTTGTTCCACAAT+AGG	0.312357	3.3:-43059752	None:intergenic
CCTGAAGAGGAAAACGTGTT+TGG	0.319867	3.3:-43058076	None:intergenic
ATGCGGCTCACATGGGAAAA+TGG	0.324844	3.3:-43060008	None:intergenic
CTCATTCTTGTTCCACAATA+GGG	0.330179	3.3:-43059751	None:intergenic
GTTCTTGAAAGCAAGAATTC+TGG	0.338489	3.3:-43058471	None:intergenic
GGATTGAAATGGTCGGCTTC+TGG	0.343844	3.3:+43059489	MS.gene30174:CDS
AATGTTCTTGCAATAGCATT+AGG	0.348040	3.3:+43059024	MS.gene30174:CDS
ATGATAATCAACTTTACATC+TGG	0.350065	3.3:+43059535	MS.gene30174:CDS
TTCCTCTTCAGGAACATTTA+CGG	0.351931	3.3:+43058087	MS.gene30174:CDS
AATGGCTCAGCCCTTGCTTT+CGG	0.361719	3.3:-43059990	None:intergenic
GCGATGAAACCATCAAGTTT+TGG	0.364930	3.3:+43059673	MS.gene30174:CDS
GTTGCCACTGTGCAACCATC+CGG	0.369143	3.3:-43059897	None:intergenic
CTGAAGAGGAAAACGTGTTT+GGG	0.370197	3.3:-43058075	None:intergenic
TTCACTCTAACAATGGCTTC+AGG	0.374235	3.3:+43058026	None:intergenic
TAGCTTCCTGTAAGCTTCTC+TGG	0.375997	3.3:-43058420	None:intergenic
GATGGCCGTCATATTGGTAT+TGG	0.386280	3.3:+43059147	MS.gene30174:CDS
GCTCCTGATGGCCGTCATAT+TGG	0.390067	3.3:+43059141	MS.gene30174:CDS
ATTTCAATCCGCAAACTTCT+CGG	0.394694	3.3:-43059476	None:intergenic
AATAATTCGCCGGGGCTTGA+TGG	0.396021	3.3:-43058567	None:intergenic
CCCTATCACATCTGTAAGTT+GGG	0.396604	3.3:+43059119	MS.gene30174:CDS
CAAGGGAATTTGCTAGCTAC+TGG	0.408913	3.3:+43059639	MS.gene30174:CDS
CTCAGTTGACACTGGGTCTC+AGG	0.408962	3.3:+43059722	MS.gene30174:CDS
CAGAAGCTTTAAACATGAAC+AGG	0.411753	3.3:+43058447	MS.gene30174:CDS
TAGGAAACACATTGTATTTG+TGG	0.412235	3.3:+43059043	MS.gene30174:CDS
CTAGCTACTGGCGGAGGCAG+TGG	0.415453	3.3:+43059651	MS.gene30174:CDS
CTCAGGTATGTTCCCTATTG+TGG	0.418001	3.3:+43059739	MS.gene30174:CDS
GCCCTATCACATCTGTAAGT+TGG	0.420766	3.3:+43059118	MS.gene30174:CDS
GAGAAGTTTGCGGATTGAAA+TGG	0.425267	3.3:+43059478	MS.gene30174:CDS
TAATAGTCATCCACAATGTC+TGG	0.428065	3.3:-43058973	None:intergenic
CTTGCTTGGTGTCCTTTCCA+AGG	0.428743	3.3:+43059621	MS.gene30174:CDS
GTGGGTATTCCAAAACTTGA+TGG	0.428833	3.3:-43059682	None:intergenic
TAGGCAGCGAGTAGGGTCTT+TGG	0.428851	3.3:+43059350	MS.gene30174:CDS
AAATCCATCACTGATCTATT+CGG	0.435009	3.3:-43058323	None:intergenic
GTCGGCTTCTGGTCTACAAT+TGG	0.444050	3.3:+43059500	MS.gene30174:CDS
GCCCAACTTACAGATGTGAT+AGG	0.450468	3.3:-43059120	None:intergenic
AGGAAACACATTGTATTTGT+GGG	0.452538	3.3:+43059044	MS.gene30174:CDS
CTTATCGAATGCGGCTCACA+TGG	0.453694	3.3:-43060016	None:intergenic
GGATACAGTTTCAGATAGAC+AGG	0.455946	3.3:+43059197	MS.gene30174:CDS
ACAACTCTGAAGTGCAGCTA+TGG	0.458171	3.3:+43059175	MS.gene30174:CDS
CTTCAACAAGGTGGCTTCAC+AGG	0.460645	3.3:+43059568	MS.gene30174:CDS
TTGCTTGGTGTCCTTTCCAA+GGG	0.471233	3.3:+43059622	MS.gene30174:CDS
CAAAAGCTGCACCGAAAGCA+AGG	0.471352	3.3:+43059979	MS.gene30174:CDS
TCTGCCATCTTCACCATTGA+GGG	0.471695	3.3:-43059831	None:intergenic
CTCCGTAAATGTTCCTGAAG+AGG	0.475527	3.3:-43058089	None:intergenic
TCTTCATATGGCACAGAGCC+CGG	0.475568	3.3:+43059878	MS.gene30174:CDS
CACAGAATCAGATAACACTT+TGG	0.480456	3.3:+43059802	MS.gene30174:CDS
TCATGAGAGAAGAAATCAAC+TGG	0.484334	3.3:-43058509	None:intergenic
TTCTGCCATCTTCACCATTG+AGG	0.488145	3.3:-43059832	None:intergenic
TTGATTATCATTCCCTCCAC+TGG	0.491219	3.3:-43059523	None:intergenic
CACTTCAAGAGTTCTTCATA+TGG	0.491466	3.3:+43059866	MS.gene30174:CDS
TGGAAGGAGAGCACACTTGT+GGG	0.493184	3.3:-43058400	None:intergenic
TGCTATTGCAAGAACATTGG+CGG	0.493579	3.3:-43059019	None:intergenic
ATACCAATATGACGGCCATC+AGG	0.497674	3.3:-43059144	None:intergenic
CGCACTACATGGTGACAGAA+GGG	0.504327	3.3:+43058354	MS.gene30174:CDS
ATATCCCTCAATGGTGAAGA+TGG	0.507244	3.3:+43059827	MS.gene30174:CDS
CTGGAAGGAGAGCACACTTG+TGG	0.508404	3.3:-43058401	None:intergenic
CACAGTGGCAACAGCTGCGG+CGG	0.509651	3.3:+43059908	MS.gene30174:CDS
AAGAAATCAACTGGCGGCGT+AGG	0.510142	3.3:-43058500	None:intergenic
TTGCCACTGTGCAACCATCC+GGG	0.511124	3.3:-43059896	None:intergenic
GGGCATGACCGAGAAGTTTG+CGG	0.518071	3.3:+43059468	MS.gene30174:CDS
CTGCAGTTGAGAACTTTGAA+AGG	0.529944	3.3:+43059321	MS.gene30174:intron
GAACTTTGAAAGGTGGGCAT+AGG	0.530523	3.3:+43059331	MS.gene30174:CDS
AATAACCACATACTCACAAC+CGG	0.531617	3.3:+43059378	MS.gene30174:CDS
TCCCTCCGGTTGTGAGTATG+TGG	0.533027	3.3:-43059383	None:intergenic
GCCCCGGCGAATTATTCCTC+AGG	0.533857	3.3:+43058574	MS.gene30174:CDS
CTGCAGTAAAAGCTCTTGCT+TGG	0.539760	3.3:+43059607	MS.gene30174:CDS
ACACCTGAGGAATAATTCGC+CGG	0.542147	3.3:-43058577	None:intergenic
TGATAATCAACTTTACATCT+GGG	0.543770	3.3:+43059536	MS.gene30174:CDS
TTTCGATTATGCGCACTACA+TGG	0.544215	3.3:+43058343	MS.gene30174:CDS
TGCTTGAACTCAGTTGACAC+TGG	0.544299	3.3:+43059714	MS.gene30174:CDS
AGGGAGCTACTCAGCTCTCA+TGG	0.545560	3.3:+43059774	MS.gene30174:CDS
AACGGTAACAAGTTCTGAAG+TGG	0.549127	3.3:-43059082	None:intergenic
CAACTCTGAAGTGCAGCTAT+GGG	0.549221	3.3:+43059176	MS.gene30174:CDS
TATTTGTGGGATGCATCAAA+CGG	0.551368	3.3:+43059057	MS.gene30174:CDS
AAATCAACTGGCGGCGTAGG+TGG	0.551699	3.3:-43058497	None:intergenic
TAATGCTATTGCAAGAACAT+TGG	0.553424	3.3:-43059022	None:intergenic
CTCACAACCGGAGGGATGGA+TGG	0.555824	3.3:+43059390	MS.gene30174:CDS
GTGGGCATAGGCAGCGAGTA+GGG	0.557831	3.3:+43059343	MS.gene30174:CDS
CACCTGAGGAATAATTCGCC+GGG	0.559324	3.3:-43058576	None:intergenic
AGTTGAGAACTTTGAAAGGT+GGG	0.559397	3.3:+43059325	MS.gene30174:intron
AGGGGCTAAAGCTAAAGGTA+AGG	0.567414	3.3:+43058373	MS.gene30174:CDS
TTCCCAAATCCAGTAAATTG+AGG	0.569854	3.3:-43058996	None:intergenic
GTCTACAATTGGCCAGTGGA+GGG	0.570881	3.3:+43059511	MS.gene30174:CDS
GCGCACTACATGGTGACAGA+AGG	0.570973	3.3:+43058353	MS.gene30174:CDS
GTGAAGATGGCAGAACTCAA+TGG	0.571074	3.3:+43059840	MS.gene30174:CDS
CATACTCACAACCGGAGGGA+TGG	0.571933	3.3:+43059386	MS.gene30174:CDS
CAGTTGAGAACTTTGAAAGG+TGG	0.572101	3.3:+43059324	MS.gene30174:intron
GGTCTACAATTGGCCAGTGG+AGG	0.575013	3.3:+43059510	MS.gene30174:CDS
ACAGAAGGGGCTAAAGCTAA+AGG	0.578064	3.3:+43058368	MS.gene30174:CDS
TTCCTGTAAGCTTCTCTGGA+AGG	0.579286	3.3:-43058416	None:intergenic
TCTGTAAGTTGGGCTCCTGA+TGG	0.584539	3.3:+43059129	MS.gene30174:CDS
CCCAACTTACAGATGTGATA+GGG	0.585245	3.3:-43059119	None:intergenic
AATTCTACCATCCATCCCTC+CGG	0.585408	3.3:-43059397	None:intergenic
AAAAGCATTCCAAAACCTCA+AGG	0.587395	3.3:-43059937	None:intergenic
ACTTTGGAAATATCCCTCAA+TGG	0.588841	3.3:+43059818	MS.gene30174:CDS
GCTAGCAAATTCCCTTGGAA+AGG	0.591056	3.3:-43059633	None:intergenic
CATATGGCACAGAGCCCGGA+TGG	0.592376	3.3:+43059882	MS.gene30174:CDS
GGTGGGCATAGGCAGCGAGT+AGG	0.596714	3.3:+43059342	MS.gene30174:CDS
AGCGAGTAGGGTCTTTGGCA+TGG	0.601597	3.3:+43059355	MS.gene30174:CDS
GTTACCGTTGATGATGAAGA+TGG	0.607070	3.3:+43059096	MS.gene30174:CDS
ATTGGATGCTCCAGACATTG+TGG	0.612151	3.3:+43058963	MS.gene30174:CDS
AAAATAAAACCATCAAGCCC+CGG	0.612311	3.3:+43058558	MS.gene30174:CDS
TATTGTGGAACAAGAATGAG+AGG	0.622948	3.3:+43059754	MS.gene30174:CDS
TTGCACAGTGGCAACAGCTG+CGG	0.623694	3.3:+43059905	MS.gene30174:CDS
TTAAGCCAATACCAATATGA+CGG	0.624041	3.3:-43059152	None:intergenic
AGGGCCATCTTCATCATCAA+CGG	0.626287	3.3:-43059100	None:intergenic
GCTTGAACTCAGTTGACACT+GGG	0.627877	3.3:+43059715	MS.gene30174:CDS
AAAAGCTGCACCGAAAGCAA+GGG	0.629505	3.3:+43059980	MS.gene30174:CDS
CAGTAGCTAGCAAATTCCCT+TGG	0.635739	3.3:-43059638	None:intergenic
CATACCGAATAGATCAGTGA+TGG	0.638668	3.3:+43058319	MS.gene30174:CDS
TCTGGTCTACAATTGGCCAG+TGG	0.642557	3.3:+43059507	MS.gene30174:CDS
ACCACATACTCACAACCGGA+GGG	0.650856	3.3:+43059382	MS.gene30174:CDS
TTATCGAATGCGGCTCACAT+GGG	0.652235	3.3:-43060015	None:intergenic
AATTTGCTAGCTACTGGCGG+AGG	0.655067	3.3:+43059645	MS.gene30174:CDS
GGGAATTTGCTAGCTACTGG+CGG	0.656204	3.3:+43059642	MS.gene30174:CDS
AGTTTGCGGATTGAAATGGT+CGG	0.668119	3.3:+43059482	MS.gene30174:CDS
GTTCAAGCATGCACCTGTGT+GGG	0.668846	3.3:-43059700	None:intergenic
TTGTTTCTCAGACTTCTGAG+AGG	0.673720	3.3:+43058938	MS.gene30174:intron
GCACTACATGGTGACAGAAG+GGG	0.674164	3.3:+43058355	MS.gene30174:CDS
CTGCGGCGGATGAAACCTTG+AGG	0.678181	3.3:+43059922	MS.gene30174:CDS
AGTTCAAGCATGCACCTGTG+TGG	0.678713	3.3:-43059701	None:intergenic
ACATTGTTGAAACATACAGA+GGG	0.688874	3.3:+43059448	MS.gene30174:CDS
AACCACATACTCACAACCGG+AGG	0.695066	3.3:+43059381	MS.gene30174:CDS
TGAGAGAAGAAATCAACTGG+CGG	0.700989	3.3:-43058506	None:intergenic
CACATTGTTGAAACATACAG+AGG	0.702329	3.3:+43059447	MS.gene30174:CDS
GGGATAGAAGTACTTCAACA+AGG	0.702662	3.3:+43059556	MS.gene30174:CDS
AAGTTACTACTTATCGAATG+CGG	0.717524	3.3:-43060025	None:intergenic
ATTGTGGAACAAGAATGAGA+GGG	0.731893	3.3:+43059755	MS.gene30174:CDS
ACCTGAGGAATAATTCGCCG+GGG	0.733838	3.3:-43058575	None:intergenic
ATAGAAGTACTTCAACAAGG+TGG	0.753913	3.3:+43059559	MS.gene30174:CDS
GAGCCCGGATGGTTGCACAG+TGG	0.759818	3.3:+43059893	MS.gene30174:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAAGAATGAATTTTGAAATT+AGG	+	chr3.3:43058179-43058198	MS.gene30174:intron	15.0%
!!!	AAGAAATTTTTTCATTGTTA+GGG	+	chr3.3:43058133-43058152	MS.gene30174:intron	15.0%
!!!	CAAGTACAAAATTTTAAAAT+CGG	-	chr3.3:43058709-43058728	None:intergenic	15.0%
!!!	TAAGAAATTTTTTCATTGTT+AGG	+	chr3.3:43058132-43058151	MS.gene30174:intron	15.0%
!!	AATATTAGAAAACTAGTAGT+AGG	-	chr3.3:43059286-43059305	None:intergenic	20.0%
!	ATAATGATGTATCGTTTGAA+GGG	-	chr3.3:43059243-43059262	None:intergenic	25.0%
!	ATGATAATCAACTTTACATC+TGG	+	chr3.3:43059535-43059554	MS.gene30174:CDS	25.0%
!	TGATAATCAACTTTACATCT+GGG	+	chr3.3:43059536-43059555	MS.gene30174:CDS	25.0%
!!	ATTAGTTTCTGAAAATCAAG+TGG	+	chr3.3:43058729-43058748	MS.gene30174:intron	25.0%
!!	CACAAAATTGCAAAATGTTT+TGG	-	chr3.3:43058902-43058921	None:intergenic	25.0%
!!	GATTTGAGAATTTTGAACTT+CGG	+	chr3.3:43058837-43058856	MS.gene30174:intron	25.0%
!!	TTAGTTTCTGAAAATCAAGT+GGG	+	chr3.3:43058730-43058749	MS.gene30174:intron	25.0%
	AAATCCATCACTGATCTATT+CGG	-	chr3.3:43058326-43058345	None:intergenic	30.0%
	AATGTTCTTGCAATAGCATT+AGG	+	chr3.3:43059024-43059043	MS.gene30174:CDS	30.0%
	ACATTGTTGAAACATACAGA+GGG	+	chr3.3:43059448-43059467	MS.gene30174:CDS	30.0%
	AGGAAACACATTGTATTTGT+GGG	+	chr3.3:43059044-43059063	MS.gene30174:CDS	30.0%
	CATAATGATGTATCGTTTGA+AGG	-	chr3.3:43059244-43059263	None:intergenic	30.0%
	TAATGCTATTGCAAGAACAT+TGG	-	chr3.3:43059025-43059044	None:intergenic	30.0%
	TACCTCAATTTACTGGATTT+GGG	+	chr3.3:43058994-43059013	MS.gene30174:CDS	30.0%
	TAGGAAACACATTGTATTTG+TGG	+	chr3.3:43059043-43059062	MS.gene30174:CDS	30.0%
	TGACTATTACCTCAATTTAC+TGG	+	chr3.3:43058987-43059006	MS.gene30174:CDS	30.0%
	TTAAGCCAATACCAATATGA+CGG	-	chr3.3:43059155-43059174	None:intergenic	30.0%
	TTACCTCAATTTACTGGATT+TGG	+	chr3.3:43058993-43059012	MS.gene30174:CDS	30.0%
	TTAGGTCATGATAACTAACT+AGG	+	chr3.3:43058197-43058216	MS.gene30174:intron	30.0%
!	TGTTTTCGTAATCGTTTGTA+TGG	+	chr3.3:43058760-43058779	MS.gene30174:intron	30.0%
!!	AGACAGGTTTTATACTTATC+TGG	+	chr3.3:43059213-43059232	MS.gene30174:intron	30.0%
!!	TCTTATCTGAAAGTGTTGTA+TGG	+	chr3.3:43058870-43058889	MS.gene30174:intron	30.0%
!!	TCTTGTGCTAATTGATTTGT+TGG	+	chr3.3:43058808-43058827	MS.gene30174:intron	30.0%
	AAAATAAAACCATCAAGCCC+CGG	+	chr3.3:43058558-43058577	MS.gene30174:CDS	35.0%
	AATAACCACATACTCACAAC+CGG	+	chr3.3:43059378-43059397	MS.gene30174:CDS	35.0%
	ATTGTGGAACAAGAATGAGA+GGG	+	chr3.3:43059755-43059774	MS.gene30174:CDS	35.0%
	ATTTCAATCCGCAAACTTCT+CGG	-	chr3.3:43059479-43059498	None:intergenic	35.0%
	CACAGAATCAGATAACACTT+TGG	+	chr3.3:43059802-43059821	MS.gene30174:CDS	35.0%
	CACATTGTTGAAACATACAG+AGG	+	chr3.3:43059447-43059466	MS.gene30174:CDS	35.0%
	CACTTCAAGAGTTCTTCATA+TGG	+	chr3.3:43059866-43059885	MS.gene30174:CDS	35.0%
	CTCATTCTTGTTCCACAATA+GGG	-	chr3.3:43059754-43059773	None:intergenic	35.0%
	GTTCTTGAAAGCAAGAATTC+TGG	-	chr3.3:43058474-43058493	None:intergenic	35.0%
	TAATAGTCATCCACAATGTC+TGG	-	chr3.3:43058976-43058995	None:intergenic	35.0%
	TATTGTGGAACAAGAATGAG+AGG	+	chr3.3:43059754-43059773	MS.gene30174:CDS	35.0%
	TATTTGTGGGATGCATCAAA+CGG	+	chr3.3:43059057-43059076	MS.gene30174:CDS	35.0%
	TCATGAGAGAAGAAATCAAC+TGG	-	chr3.3:43058512-43058531	None:intergenic	35.0%
	TCTCATTCTTGTTCCACAAT+AGG	-	chr3.3:43059755-43059774	None:intergenic	35.0%
	TTCCCAAATCCAGTAAATTG+AGG	-	chr3.3:43058999-43059018	None:intergenic	35.0%
	TTCCTCTTCAGGAACATTTA+CGG	+	chr3.3:43058087-43058106	MS.gene30174:CDS	35.0%
!	ACTTTGGAAATATCCCTCAA+TGG	+	chr3.3:43059818-43059837	MS.gene30174:CDS	35.0%
!	AGTTGAGAACTTTGAAAGGT+GGG	+	chr3.3:43059325-43059344	MS.gene30174:intron	35.0%
!!	AAAACGTGTTTGGGTTTTGA+TGG	-	chr3.3:43058069-43058088	None:intergenic	35.0%
!!	AAAAGCATTCCAAAACCTCA+AGG	-	chr3.3:43059940-43059959	None:intergenic	35.0%
!!	ATAGAAGTACTTCAACAAGG+TGG	+	chr3.3:43059559-43059578	MS.gene30174:CDS	35.0%
!!	CAGAAGCTTTAAACATGAAC+AGG	+	chr3.3:43058447-43058466	MS.gene30174:CDS	35.0%
!!	TTGATTTGTCTGTTACCAGT+TGG	+	chr3.3:43058289-43058308	MS.gene30174:intron	35.0%
!!!	TTGAGGTTTTGGAATGCTTT+TGG	+	chr3.3:43059939-43059958	MS.gene30174:CDS	35.0%
	ATATCCCTCAATGGTGAAGA+TGG	+	chr3.3:43059827-43059846	MS.gene30174:CDS	40.0%
	CATACCGAATAGATCAGTGA+TGG	+	chr3.3:43058319-43058338	MS.gene30174:CDS	40.0%
	CCCAACTTACAGATGTGATA+GGG	-	chr3.3:43059122-43059141	None:intergenic	40.0%
	CCCTATCACATCTGTAAGTT+GGG	+	chr3.3:43059119-43059138	MS.gene30174:CDS	40.0%
	CTCAACTGCAGAAACATAAC+AGG	-	chr3.3:43059312-43059331	None:intergenic	40.0%
	CTGAAGAGGAAAACGTGTTT+GGG	-	chr3.3:43058078-43058097	None:intergenic	40.0%
	CTGCAGTTGAGAACTTTGAA+AGG	+	chr3.3:43059321-43059340	MS.gene30174:intron	40.0%
	GGATACAGTTTCAGATAGAC+AGG	+	chr3.3:43059197-43059216	MS.gene30174:CDS	40.0%
	GTGGGTATTCCAAAACTTGA+TGG	-	chr3.3:43059685-43059704	None:intergenic	40.0%
	TCGGTATGAATCTATCCAAC+TGG	-	chr3.3:43058307-43058326	None:intergenic	40.0%
	TGAGAGAAGAAATCAACTGG+CGG	-	chr3.3:43058509-43058528	None:intergenic	40.0%
	TGCTATTGCAAGAACATTGG+CGG	-	chr3.3:43059022-43059041	None:intergenic	40.0%
	TTGATTATCATTCCCTCCAC+TGG	-	chr3.3:43059526-43059545	None:intergenic	40.0%
	TTTCGATTATGCGCACTACA+TGG	+	chr3.3:43058343-43058362	MS.gene30174:CDS	40.0%
!	AGTTTGCGGATTGAAATGGT+CGG	+	chr3.3:43059482-43059501	MS.gene30174:CDS	40.0%
!	CAGTTGAGAACTTTGAAAGG+TGG	+	chr3.3:43059324-43059343	MS.gene30174:intron	40.0%
!	GAGAAGTTTGCGGATTGAAA+TGG	+	chr3.3:43059478-43059497	MS.gene30174:CDS	40.0%
!	GCGATGAAACCATCAAGTTT+TGG	+	chr3.3:43059673-43059692	MS.gene30174:CDS	40.0%
!	TCAGACTTCTGAGAGGATAT+TGG	+	chr3.3:43058945-43058964	MS.gene30174:intron	40.0%
!	TTGTTTCTCAGACTTCTGAG+AGG	+	chr3.3:43058938-43058957	MS.gene30174:intron	40.0%
!!	AACGGTAACAAGTTCTGAAG+TGG	-	chr3.3:43059085-43059104	None:intergenic	40.0%
!!	GGGATAGAAGTACTTCAACA+AGG	+	chr3.3:43059556-43059575	MS.gene30174:CDS	40.0%
!!	GTTACCGTTGATGATGAAGA+TGG	+	chr3.3:43059096-43059115	MS.gene30174:CDS	40.0%
!!!	AAGTTTTGGAATACCCACAC+AGG	+	chr3.3:43059687-43059706	MS.gene30174:CDS	40.0%
	AAAAGCTGCACCGAAAGCAA+GGG	+	chr3.3:43059980-43059999	MS.gene30174:CDS	45.0%
	AAGAAACACACACACACCTG+AGG	-	chr3.3:43058593-43058612	None:intergenic	45.0%
	AATTCTACCATCCATCCCTC+CGG	-	chr3.3:43059400-43059419	None:intergenic	45.0%
	ACACCTGAGGAATAATTCGC+CGG	-	chr3.3:43058580-43058599	None:intergenic	45.0%
	ACAGAAGGGGCTAAAGCTAA+AGG	+	chr3.3:43058368-43058387	MS.gene30174:CDS	45.0%
	AGGGCCATCTTCATCATCAA+CGG	-	chr3.3:43059103-43059122	None:intergenic	45.0%
	AGGGGCTAAAGCTAAAGGTA+AGG	+	chr3.3:43058373-43058392	MS.gene30174:CDS	45.0%
	ATACCAATATGACGGCCATC+AGG	-	chr3.3:43059147-43059166	None:intergenic	45.0%
	ATTGGATGCTCCAGACATTG+TGG	+	chr3.3:43058963-43058982	MS.gene30174:CDS	45.0%
	CAGTAGCTAGCAAATTCCCT+TGG	-	chr3.3:43059641-43059660	None:intergenic	45.0%
	CCTGAAGAGGAAAACGTGTT+TGG	-	chr3.3:43058079-43058098	None:intergenic	45.0%
	CTCAGGTATGTTCCCTATTG+TGG	+	chr3.3:43059739-43059758	MS.gene30174:CDS	45.0%
	CTCCGTAAATGTTCCTGAAG+AGG	-	chr3.3:43058092-43058111	None:intergenic	45.0%
	CTGCAGTAAAAGCTCTTGCT+TGG	+	chr3.3:43059607-43059626	MS.gene30174:CDS	45.0%
	GATGGCCGTCATATTGGTAT+TGG	+	chr3.3:43059147-43059166	MS.gene30174:CDS	45.0%
	GCCCAACTTACAGATGTGAT+AGG	-	chr3.3:43059123-43059142	None:intergenic	45.0%
	GCCCTATCACATCTGTAAGT+TGG	+	chr3.3:43059118-43059137	MS.gene30174:CDS	45.0%
	GCTAGCAAATTCCCTTGGAA+AGG	-	chr3.3:43059636-43059655	None:intergenic	45.0%
	GCTTGAACTCAGTTGACACT+GGG	+	chr3.3:43059715-43059734	MS.gene30174:CDS	45.0%
	GTGAAGATGGCAGAACTCAA+TGG	+	chr3.3:43059840-43059859	MS.gene30174:CDS	45.0%
	TAGCTTCCTGTAAGCTTCTC+TGG	-	chr3.3:43058423-43058442	None:intergenic	45.0%
	TCTGCCATCTTCACCATTGA+GGG	-	chr3.3:43059834-43059853	None:intergenic	45.0%
	TGCTTGAACTCAGTTGACAC+TGG	+	chr3.3:43059714-43059733	MS.gene30174:CDS	45.0%
	TTATCGAATGCGGCTCACAT+GGG	-	chr3.3:43060018-43060037	None:intergenic	45.0%
	TTCCTGTAAGCTTCTCTGGA+AGG	-	chr3.3:43058419-43058438	None:intergenic	45.0%
	TTCTGCCATCTTCACCATTG+AGG	-	chr3.3:43059835-43059854	None:intergenic	45.0%
!	CCAAACACGTTTTCCTCTTC+AGG	+	chr3.3:43058076-43058095	MS.gene30174:CDS	45.0%
!	CGGATGAAACCTTGAGGTTT+TGG	+	chr3.3:43059928-43059947	MS.gene30174:CDS	45.0%
!	GAACTTTGAAAGGTGGGCAT+AGG	+	chr3.3:43059331-43059350	MS.gene30174:CDS	45.0%
!!	ACAACTCTGAAGTGCAGCTA+TGG	+	chr3.3:43059175-43059194	MS.gene30174:CDS	45.0%
!!	CAACTCTGAAGTGCAGCTAT+GGG	+	chr3.3:43059176-43059195	MS.gene30174:CDS	45.0%
!!	CAAGGGAATTTGCTAGCTAC+TGG	+	chr3.3:43059639-43059658	MS.gene30174:CDS	45.0%
!!	GCAGCTTTTGTTGCTACTTC+TGG	-	chr3.3:43059969-43059988	None:intergenic	45.0%
!!	TTGCTTGGTGTCCTTTCCAA+GGG	+	chr3.3:43059622-43059641	MS.gene30174:CDS	45.0%
!!!	TTATTTTATCATTATTAATT+TGG	+	chr3.3:43058636-43058655	MS.gene30174:intron	5.0%
	AACCACATACTCACAACCGG+AGG	+	chr3.3:43059381-43059400	MS.gene30174:CDS	50.0%
	AAGAAATCAACTGGCGGCGT+AGG	-	chr3.3:43058503-43058522	None:intergenic	50.0%
	AATAATTCGCCGGGGCTTGA+TGG	-	chr3.3:43058570-43058589	None:intergenic	50.0%
	AATGGCTCAGCCCTTGCTTT+CGG	-	chr3.3:43059993-43060012	None:intergenic	50.0%
	ACCACATACTCACAACCGGA+GGG	+	chr3.3:43059382-43059401	MS.gene30174:CDS	50.0%
	ACCTGAGGAATAATTCGCCG+GGG	-	chr3.3:43058578-43058597	None:intergenic	50.0%
	AGTTCAAGCATGCACCTGTG+TGG	-	chr3.3:43059704-43059723	None:intergenic	50.0%
	ATGCGGCTCACATGGGAAAA+TGG	-	chr3.3:43060011-43060030	None:intergenic	50.0%
	CAAAAGCTGCACCGAAAGCA+AGG	+	chr3.3:43059979-43059998	MS.gene30174:CDS	50.0%
	CACCTGAGGAATAATTCGCC+GGG	-	chr3.3:43058579-43058598	None:intergenic	50.0%
	CGCACTACATGGTGACAGAA+GGG	+	chr3.3:43058354-43058373	MS.gene30174:CDS	50.0%
	CTCCTTCCAGAGAAGCTTAC+AGG	+	chr3.3:43058414-43058433	MS.gene30174:CDS	50.0%
	CTTCAACAAGGTGGCTTCAC+AGG	+	chr3.3:43059568-43059587	MS.gene30174:CDS	50.0%
	GCACTACATGGTGACAGAAG+GGG	+	chr3.3:43058355-43058374	MS.gene30174:CDS	50.0%
	GTCTACAATTGGCCAGTGGA+GGG	+	chr3.3:43059511-43059530	MS.gene30174:CDS	50.0%
	GTTCAAGCATGCACCTGTGT+GGG	-	chr3.3:43059703-43059722	None:intergenic	50.0%
	TCTGGTCTACAATTGGCCAG+TGG	+	chr3.3:43059507-43059526	MS.gene30174:CDS	50.0%
	TCTTCATATGGCACAGAGCC+CGG	+	chr3.3:43059878-43059897	MS.gene30174:CDS	50.0%
!	GGATTGAAATGGTCGGCTTC+TGG	+	chr3.3:43059489-43059508	MS.gene30174:CDS	50.0%
!	GTCGGCTTCTGGTCTACAAT+TGG	+	chr3.3:43059500-43059519	MS.gene30174:CDS	50.0%
!	TCTGTAAGTTGGGCTCCTGA+TGG	+	chr3.3:43059129-43059148	MS.gene30174:CDS	50.0%
!	TGGAAGGAGAGCACACTTGT+GGG	-	chr3.3:43058403-43058422	None:intergenic	50.0%
!!	AATTTGCTAGCTACTGGCGG+AGG	+	chr3.3:43059645-43059664	MS.gene30174:CDS	50.0%
!!	CTTGCTTGGTGTCCTTTCCA+AGG	+	chr3.3:43059621-43059640	MS.gene30174:CDS	50.0%
!!	GGGAATTTGCTAGCTACTGG+CGG	+	chr3.3:43059642-43059661	MS.gene30174:CDS	50.0%
	AAATCAACTGGCGGCGTAGG+TGG	-	chr3.3:43058500-43058519	None:intergenic	55.0%
	AGCGAGTAGGGTCTTTGGCA+TGG	+	chr3.3:43059355-43059374	MS.gene30174:CDS	55.0%
	AGGGAGCTACTCAGCTCTCA+TGG	+	chr3.3:43059774-43059793	MS.gene30174:CDS	55.0%
	CATACTCACAACCGGAGGGA+TGG	+	chr3.3:43059386-43059405	MS.gene30174:CDS	55.0%
	CTCAGTTGACACTGGGTCTC+AGG	+	chr3.3:43059722-43059741	MS.gene30174:CDS	55.0%
	GCGCACTACATGGTGACAGA+AGG	+	chr3.3:43058353-43058372	MS.gene30174:CDS	55.0%
	GCTCCTGATGGCCGTCATAT+TGG	+	chr3.3:43059141-43059160	MS.gene30174:CDS	55.0%
	GGGCATGACCGAGAAGTTTG+CGG	+	chr3.3:43059468-43059487	MS.gene30174:CDS	55.0%
	GGTCTACAATTGGCCAGTGG+AGG	+	chr3.3:43059510-43059529	MS.gene30174:CDS	55.0%
	GTTGCCACTGTGCAACCATC+CGG	-	chr3.3:43059900-43059919	None:intergenic	55.0%
	TAGGCAGCGAGTAGGGTCTT+TGG	+	chr3.3:43059350-43059369	MS.gene30174:CDS	55.0%
	TCCCTCCGGTTGTGAGTATG+TGG	-	chr3.3:43059386-43059405	None:intergenic	55.0%
	TTGCACAGTGGCAACAGCTG+CGG	+	chr3.3:43059905-43059924	MS.gene30174:CDS	55.0%
	TTGCCACTGTGCAACCATCC+GGG	-	chr3.3:43059899-43059918	None:intergenic	55.0%
!	CTGGAAGGAGAGCACACTTG+TGG	-	chr3.3:43058404-43058423	None:intergenic	55.0%
	CATATGGCACAGAGCCCGGA+TGG	+	chr3.3:43059882-43059901	MS.gene30174:CDS	60.0%
	CTCACAACCGGAGGGATGGA+TGG	+	chr3.3:43059390-43059409	MS.gene30174:CDS	60.0%
	CTGCGGCGGATGAAACCTTG+AGG	+	chr3.3:43059922-43059941	MS.gene30174:CDS	60.0%
	GCCCCGGCGAATTATTCCTC+AGG	+	chr3.3:43058574-43058593	MS.gene30174:CDS	60.0%
	GTGGGCATAGGCAGCGAGTA+GGG	+	chr3.3:43059343-43059362	MS.gene30174:CDS	60.0%
	CACAGTGGCAACAGCTGCGG+CGG	+	chr3.3:43059908-43059927	MS.gene30174:CDS	65.0%
	CTAGCTACTGGCGGAGGCAG+TGG	+	chr3.3:43059651-43059670	MS.gene30174:CDS	65.0%
	GAGCCCGGATGGTTGCACAG+TGG	+	chr3.3:43059893-43059912	MS.gene30174:CDS	65.0%
	GGTGGGCATAGGCAGCGAGT+AGG	+	chr3.3:43059342-43059361	MS.gene30174:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	43058038	43060037	43058038	ID=MS.gene30174
chr3.3	mRNA	43058038	43060037	43058038	ID=MS.gene30174.t1;Parent=MS.gene30174
chr3.3	exon	43058038	43058130	43058038	ID=MS.gene30174.t1.exon1;Parent=MS.gene30174.t1
chr3.3	CDS	43058038	43058130	43058038	ID=cds.MS.gene30174.t1;Parent=MS.gene30174.t1
chr3.3	exon	43058308	43058595	43058308	ID=MS.gene30174.t1.exon2;Parent=MS.gene30174.t1
chr3.3	CDS	43058308	43058595	43058308	ID=cds.MS.gene30174.t1;Parent=MS.gene30174.t1
chr3.3	exon	43058949	43059218	43058949	ID=MS.gene30174.t1.exon3;Parent=MS.gene30174.t1
chr3.3	CDS	43058949	43059218	43058949	ID=cds.MS.gene30174.t1;Parent=MS.gene30174.t1
chr3.3	exon	43059327	43060037	43059327	ID=MS.gene30174.t1.exon4;Parent=MS.gene30174.t1
chr3.3	CDS	43059327	43060037	43059327	ID=cds.MS.gene30174.t1;Parent=MS.gene30174.t1

Gene Sequence

>MS.gene30174

ATGGCTTCAGGTTCTTTGAGTTCTTCTGCCATCAAAACCCAAACACGTTTTCCTCTTCAGGAACATTTACGGAGAAAGACTTCAAATGAAAATGTAAGAAATTTTTTCATTGTTAGGGTTTCAATGTAATTCTGTTTTGAAAAAGAATGAATTTTGAAATTAGGTCATGATAACTAACTAGGTACTTTATTTTGTGAGAATTTCTTTATAAAAATCAAGTAGTTTATTATAAAAAGAATGTAATTTGATGTTTGATTTGTCTGTTACCAGTTGGATAGATTCATACCGAATAGATCAGTGATGGATTTCGATTATGCGCACTACATGGTGACAGAAGGGGCTAAAGCTAAAGGTAAGGAAAACCCACAAGTGTGCTCTCCTTCCAGAGAAGCTTACAGGAAGCTACTTGCAGAAGCTTTAAACATGAACAGGACCAGAATTCTTGCTTTCAAGAACAAGCCACCTACGCCGCCAGTTGATTTCTTCTCTCATGATATCATTACTTCATCAACTCTAAAACAAAATAAAACCATCAAGCCCCGGCGAATTATTCCTCAGGTGTGTGTGTGTTTCTTAATGATATCTATTCATATTTCATTTATTTTATCATTATTAATTTGGTTGCATGAACTCTAGTTTAAATGAACTCTAGTTTAAACAGCAAAAGTCCGATTTTAAAATTTTGTACTTGATTAGTTTCTGAAAATCAAGTGGGTCTGAGTTGTTTTCGTAATCGTTTGTATGGTGTGTGTAATCACAATAGTTTGCAATCTTGTGCTAATTGATTTGTTGGTTGTCTGATTTGAGAATTTTGAACTTCGGTTTTCTTAATTCTTATCTGAAAGTGTTGTATGGTGTAAACCAAAACATTTTGCAATTTTGTGCTAATTGATTGATATGTTGTTTCTCAGACTTCTGAGAGGATATTGGATGCTCCAGACATTGTGGATGACTATTACCTCAATTTACTGGATTTGGGAACCGCCAATGTTCTTGCAATAGCATTAGGAAACACATTGTATTTGTGGGATGCATCAAACGGTTCCACTTCAGAACTTGTTACCGTTGATGATGAAGATGGCCCTATCACATCTGTAAGTTGGGCTCCTGATGGCCGTCATATTGGTATTGGCTTAAACAACTCTGAAGTGCAGCTATGGGATACAGTTTCAGATAGACAGGTTTTATACTTATCTGGTTTACCCTTCAAACGATACATCATTATGAGCTATTGAGTATCTAATTCCTACTACTAGTTTTCTAATATTATTCCTGTTATGTTTCTGCAGTTGAGAACTTTGAAAGGTGGGCATAGGCAGCGAGTAGGGTCTTTGGCATGGAATAACCACATACTCACAACCGGAGGGATGGATGGTAGAATTATCAATAATGATGTAAGAATTAGAGCACACATTGTTGAAACATACAGAGGGCATGACCGAGAAGTTTGCGGATTGAAATGGTCGGCTTCTGGTCTACAATTGGCCAGTGGAGGGAATGATAATCAACTTTACATCTGGGATAGAAGTACTTCAACAAGGTGGCTTCACAGGCTTGAAGATCATACATCTGCAGTAAAAGCTCTTGCTTGGTGTCCTTTCCAAGGGAATTTGCTAGCTACTGGCGGAGGCAGTGGCGATGAAACCATCAAGTTTTGGAATACCCACACAGGTGCATGCTTGAACTCAGTTGACACTGGGTCTCAGGTATGTTCCCTATTGTGGAACAAGAATGAGAGGGAGCTACTCAGCTCTCATGGTTTTACACAGAATCAGATAACACTTTGGAAATATCCCTCAATGGTGAAGATGGCAGAACTCAATGGTCACACTTCAAGAGTTCTTCATATGGCACAGAGCCCGGATGGTTGCACAGTGGCAACAGCTGCGGCGGATGAAACCTTGAGGTTTTGGAATGCTTTTGGCACGCCAGAAGTAGCAACAAAAGCTGCACCGAAAGCAAGGGCTGAGCCATTTTCCCATGTGAGCCGCATTCGATAA

Protein sequence

>MS.gene30174.t1

MASGSLSSSAIKTQTRFPLQEHLRRKTSNENLDRFIPNRSVMDFDYAHYMVTEGAKAKGKENPQVCSPSREAYRKLLAEALNMNRTRILAFKNKPPTPPVDFFSHDIITSSTLKQNKTIKPRRIIPQTSERILDAPDIVDDYYLNLLDLGTANVLAIALGNTLYLWDASNGSTSELVTVDDEDGPITSVSWAPDGRHIGIGLNNSEVQLWDTVSDRQLRTLKGGHRQRVGSLAWNNHILTTGGMDGRIINNDVRIRAHIVETYRGHDREVCGLKWSASGLQLASGGNDNQLYIWDRSTSTRWLHRLEDHTSAVKALAWCPFQGNLLATGGGSGDETIKFWNTHTGACLNSVDTGSQVCSLLWNKNERELLSSHGFTQNQITLWKYPSMVKMAELNGHTSRVLHMAQSPDGCTVATAAADETLRFWNAFGTPEVATKAAPKARAEPFSHVSRIR