AlfalfaGEDB | MS.gene30176

M. sativa cultivar XinJiangDaYe / MS.gene30176

KEGG: K03363
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00400.35 PF12894.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30176.t1	XP_003600092.1	93.7	459	23	2	1	453	1	459	3.40E-254	887.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30176.t1	Q9SZA4	76.4	453	98	4	7	453	8	457	4.0e-203	708.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30176.t1	G7IY01	93.7	459	23	2	1	453	1	459	2.5e-254	887.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene30176	MS.gene037184	PPI
MS.gene69965	MS.gene30176	PPI
MS.gene82740	MS.gene30176	PPI
MS.gene30176	MS.gene038519	PPI
MS.gene024753	MS.gene30176	PPI
MS.gene30176	MS.gene045133	PPI
MS.gene30176	MS.gene042783	PPI
MS.gene78281	MS.gene30176	PPI
MS.gene55882	MS.gene30176	PPI
MS.gene30176	MS.gene20160	PPI
MS.gene30172	MS.gene30176	PPI
MS.gene067322	MS.gene30176	PPI
MS.gene060496	MS.gene30176	PPI
MS.gene30176	MS.gene26021	PPI
MS.gene69966	MS.gene30176	PPI
MS.gene24806	MS.gene30176	PPI
MS.gene071113	MS.gene30176	PPI
MS.gene30176	MS.gene050456	PPI
MS.gene027071	MS.gene30176	PPI
MS.gene30176	MS.gene51258	PPI
MS.gene69396	MS.gene30176	PPI
MS.gene30176	MS.gene053363	PPI
MS.gene30176	MS.gene91775	PPI
MS.gene012203	MS.gene30176	PPI
MS.gene30176	MS.gene34651	PPI
MS.gene30176	MS.gene75493	PPI
MS.gene010910	MS.gene30176	PPI
MS.gene066464	MS.gene30176	PPI
MS.gene30176	MS.gene32726	PPI
MS.gene30176	MS.gene71122	PPI
MS.gene063222	MS.gene30176	PPI
MS.gene060495	MS.gene30176	PPI
MS.gene07706	MS.gene30176	PPI
MS.gene027074	MS.gene30176	PPI
MS.gene30176	MS.gene052956	PPI
MS.gene30176	MS.gene51262	PPI
MS.gene30176	MS.gene24951	PPI
MS.gene30176	MS.gene26672	PPI
MS.gene010912	MS.gene30176	PPI
MS.gene30176	MS.gene004844	PPI
MS.gene30176	MS.gene99588	PPI
MS.gene23811	MS.gene30176	PPI
MS.gene30176	MS.gene21888	PPI
MS.gene23810	MS.gene30176	PPI
MS.gene46611	MS.gene30176	PPI
MS.gene30176	MS.gene066500	PPI
MS.gene51335	MS.gene30176	PPI
MS.gene82021	MS.gene30176	PPI
MS.gene30176	MS.gene27223	PPI
MS.gene81658	MS.gene30176	PPI
MS.gene84465	MS.gene30176	PPI
MS.gene037950	MS.gene30176	PPI
MS.gene30176	MS.gene014419	PPI
MS.gene30176	MS.gene26019	PPI
MS.gene30176	MS.gene022109	PPI
MS.gene068249	MS.gene30176	PPI
MS.gene30176	MS.gene050272	PPI
MS.gene066046	MS.gene30176	PPI
MS.gene64441	MS.gene30176	PPI
MS.gene30176	MS.gene98540	PPI
MS.gene30176	MS.gene62819	PPI
MS.gene52355	MS.gene30176	PPI
MS.gene30176	MS.gene65775	PPI
MS.gene30176	MS.gene32723	PPI
MS.gene30176	MS.gene80163	PPI
MS.gene30176	MS.gene067321	PPI
MS.gene30176	MS.gene29842	PPI
MS.gene30176	MS.gene55883	PPI
MS.gene30176	MS.gene033401	PPI
MS.gene30176	MS.gene024753	PPI
MS.gene30176	MS.gene045937	PPI
MS.gene30176	MS.gene068273	PPI
MS.gene29768	MS.gene30176	PPI
MS.gene30176	MS.gene06177	PPI
MS.gene30176	MS.gene51278	PPI
MS.gene30176	MS.gene87218	PPI
MS.gene20161	MS.gene30176	PPI
MS.gene30176	MS.gene85534	PPI
MS.gene24922	MS.gene30176	PPI
MS.gene33406	MS.gene30176	PPI
MS.gene30176	MS.gene014321	PPI
MS.gene066467	MS.gene30176	PPI
MS.gene30176	MS.gene75059	PPI
MS.gene30176	MS.gene064862	PPI
MS.gene30176	MS.gene23881	PPI
MS.gene30176	MS.gene06176	PPI
MS.gene30176	MS.gene014931	PPI
MS.gene30176	MS.gene062662	PPI
MS.gene30176	MS.gene97379	PPI
MS.gene92674	MS.gene30176	PPI
MS.gene30176	MS.gene30177	PPI
MS.gene30176	MS.gene84466	PPI
MS.gene064035	MS.gene30176	PPI
MS.gene30176	MS.gene70008	PPI
MS.gene36842	MS.gene30176	PPI
MS.gene99554	MS.gene30176	PPI
MS.gene30176	MS.gene82740	PPI
MS.gene058128	MS.gene30176	PPI
MS.gene41291	MS.gene30176	PPI
MS.gene011448	MS.gene30176	PPI
MS.gene066501	MS.gene30176	PPI
MS.gene66342	MS.gene30176	PPI
MS.gene63085	MS.gene30176	PPI
MS.gene056455	MS.gene30176	PPI
MS.gene30176	MS.gene33377	PPI
MS.gene030294	MS.gene30176	PPI
MS.gene064762	MS.gene30176	PPI
MS.gene30176	MS.gene027073	PPI
MS.gene30176	MS.gene027072	PPI
MS.gene30176	MS.gene65463	PPI
MS.gene91851	MS.gene30176	PPI
MS.gene35613	MS.gene30176	PPI
MS.gene30176	MS.gene052188	PPI
MS.gene039682	MS.gene30176	PPI
MS.gene30176	MS.gene067705	PPI
MS.gene30176	MS.gene025229	PPI
MS.gene82020	MS.gene30176	PPI
MS.gene30176	MS.gene37226	PPI
MS.gene21734	MS.gene30176	PPI
MS.gene30176	MS.gene049021	PPI
MS.gene30176	MS.gene043742	PPI
MS.gene30176	MS.gene027075	PPI
MS.gene30176	MS.gene027441	PPI
MS.gene30176	MS.gene022502	PPI
MS.gene30176	MS.gene050457	PPI
MS.gene30176	MS.gene81660	PPI
MS.gene30176	MS.gene66343	PPI
MS.gene30176	MS.gene99973	PPI
MS.gene71204	MS.gene30176	PPI
MS.gene30176	MS.gene30174	PPI
MS.gene23852	MS.gene30176	PPI
MS.gene97943	MS.gene30176	PPI
MS.gene77885	MS.gene30176	PPI
MS.gene30176	MS.gene008833	PPI
MS.gene30176	MS.gene064435	PPI
MS.gene67157	MS.gene30176	PPI
MS.gene90626	MS.gene30176	PPI
MS.gene42253	MS.gene30176	PPI
MS.gene30176	MS.gene48655	PPI
MS.gene30176	MS.gene25515	PPI
MS.gene25244	MS.gene30176	PPI
MS.gene30176	MS.gene81663	PPI
MS.gene30176	MS.gene050270	PPI
MS.gene89986	MS.gene30176	PPI
MS.gene069995	MS.gene30176	PPI
MS.gene30176	MS.gene34712	PPI
MS.gene049022	MS.gene30176	PPI
MS.gene69687	MS.gene30176	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30176.t1	MTR_3g051690	93.682	459	23	2	1	453	1	459	0.0	895
MS.gene30176.t1	MTR_5g008010	89.301	458	40	4	1	453	1	454	0.0	846
MS.gene30176.t1	MTR_7g106870	72.727	451	110	7	12	453	11	457	0.0	681
MS.gene30176.t1	MTR_7g451830	65.324	447	129	9	15	453	3	431	0.0	571
MS.gene30176.t1	MTR_7g047640	65.324	447	129	9	15	453	3	431	0.0	571
MS.gene30176.t1	MTR_7g057540	63.616	448	132	9	14	453	2	426	0.0	543
MS.gene30176.t1	MTR_7g055590	63.535	447	132	9	14	452	2	425	0.0	540
MS.gene30176.t1	MTR_7g053100	60.398	452	149	9	15	453	2	436	0.0	523
MS.gene30176.t1	MTR_7g034625	70.440	318	87	2	128	438	13	330	2.72e-166	471
MS.gene30176.t1	MTR_2g100540	50.114	439	164	12	25	453	4	397	1.78e-128	377
MS.gene30176.t1	MTR_2g100460	56.851	343	134	5	111	451	52	382	2.04e-126	372
MS.gene30176.t1	MTR_2g060710	44.282	411	207	8	33	429	26	428	1.45e-119	357
MS.gene30176.t1	MTR_3g067940	43.810	420	214	7	33	440	57	466	9.48e-115	345
MS.gene30176.t1	MTR_4g102510	42.243	419	210	6	33	432	51	456	7.28e-109	330
MS.gene30176.t1	MTR_4g415070	52.941	68	32	0	378	445	57	124	1.06e-16	81.3
MS.gene30176.t1	MTR_2g012630	27.966	236	156	10	144	373	18	245	6.82e-16	80.9
MS.gene30176.t1	MTR_2g012630	27.966	236	156	10	144	373	18	245	7.51e-16	80.9
MS.gene30176.t1	MTR_1g054300	26.263	297	161	10	178	427	114	399	2.79e-15	78.2
MS.gene30176.t1	MTR_1g054300	26.119	268	170	11	165	425	235	481	2.30e-14	75.5
MS.gene30176.t1	MTR_6g004040	28.079	203	137	7	171	370	47	243	8.46e-15	77.4
MS.gene30176.t1	MTR_5g016370	23.345	287	164	8	147	425	312	550	5.87e-14	74.3
MS.gene30176.t1	MTR_5g016350	22.917	288	178	8	141	426	230	475	1.89e-12	69.7
MS.gene30176.t1	MTR_5g016350	22.997	287	177	8	141	425	294	538	1.98e-12	69.7
MS.gene30176.t1	MTR_3g106080	23.494	332	212	10	135	434	302	623	4.25e-12	68.6
MS.gene30176.t1	MTR_4g095058	25.000	252	169	10	186	426	292	534	9.21e-12	67.4
MS.gene30176.t1	MTR_4g095058	25.000	252	169	10	186	426	290	532	1.02e-11	67.4
MS.gene30176.t1	MTR_7g086360	23.415	205	150	4	224	426	53	252	2.76e-11	65.1
MS.gene30176.t1	MTR_1g049000	39.362	94	47	3	204	288	8	100	4.60e-11	62.4
MS.gene30176.t1	MTR_4g119620	22.581	248	177	7	186	425	81	321	5.64e-11	63.9
MS.gene30176.t1	MTR_5g022690	27.011	174	120	5	170	341	266	434	9.87e-11	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30176.t1	AT4G33270	76.380	453	98	5	7	453	8	457	0.0	713
MS.gene30176.t1	AT4G33260	76.577	444	95	5	16	453	7	447	0.0	687
MS.gene30176.t1	AT4G33260	74.707	427	99	5	16	436	7	430	0.0	654
MS.gene30176.t1	AT5G27570	67.692	455	131	7	7	453	4	450	0.0	614
MS.gene30176.t1	AT5G27080	66.970	439	128	7	23	453	13	442	0.0	591
MS.gene30176.t1	AT5G26900	64.823	452	142	7	9	453	3	444	0.0	582
MS.gene30176.t1	AT5G27945	61.186	371	124	5	99	453	62	428	2.05e-156	450
MS.gene30176.t1	AT4G22910	42.754	414	212	7	33	432	62	464	1.31e-111	337
MS.gene30176.t1	AT4G11920	41.882	425	213	8	33	438	53	462	2.76e-109	331
MS.gene30176.t1	AT5G13840	50.820	305	147	3	131	435	164	465	9.04e-109	330
MS.gene30176.t1	AT5G13840	50.820	305	147	3	131	435	164	465	9.04e-109	330
MS.gene30176.t1	AT5G08390	29.060	234	136	10	140	370	37	243	8.05e-16	80.5
MS.gene30176.t1	AT1G11160	28.641	206	134	8	144	344	18	215	9.02e-16	80.5
MS.gene30176.t1	AT1G11160	28.641	206	134	8	144	344	18	215	9.27e-16	80.5
MS.gene30176.t1	AT1G11160	28.641	206	134	8	144	344	18	215	1.01e-15	80.5
MS.gene30176.t1	AT1G11160	30.247	162	105	6	185	344	18	173	4.80e-15	78.2
MS.gene30176.t1	AT1G61210	27.830	212	140	9	144	350	18	221	1.30e-14	77.0
MS.gene30176.t1	AT1G61210	27.230	213	126	7	140	350	36	221	1.42e-14	77.0
MS.gene30176.t1	AT1G61210	27.230	213	126	7	140	350	36	221	1.43e-14	77.0
MS.gene30176.t1	AT5G52820	28.287	251	150	13	186	427	244	473	4.66e-14	74.3
MS.gene30176.t1	AT5G23430	28.169	213	124	8	140	350	37	222	1.49e-13	73.6
MS.gene30176.t1	AT5G23430	28.169	213	124	8	140	350	37	222	1.49e-13	73.6
MS.gene30176.t1	AT5G23430	28.169	213	124	8	140	350	37	222	1.65e-13	73.6
MS.gene30176.t1	AT5G25150	25.749	334	209	12	135	440	305	627	2.46e-13	72.8
MS.gene30176.t1	AT5G25150	25.749	334	209	12	135	440	305	627	2.90e-13	72.4
MS.gene30176.t1	AT2G32700	25.610	246	163	11	186	425	553	784	7.28e-13	71.2
MS.gene30176.t1	AT2G32700	25.610	246	163	11	186	425	555	786	8.03e-13	71.2
MS.gene30176.t1	AT2G32700	25.610	246	163	11	186	425	555	786	8.03e-13	71.2
MS.gene30176.t1	AT2G32700	25.610	246	163	11	186	425	555	786	8.03e-13	71.2
MS.gene30176.t1	AT2G32700	25.610	246	163	11	186	425	555	786	8.03e-13	71.2
MS.gene30176.t1	AT2G32700	25.610	246	163	11	186	425	555	786	8.03e-13	71.2
MS.gene30176.t1	AT2G32700	25.610	246	163	11	186	425	574	805	8.42e-13	71.2
MS.gene30176.t1	AT3G49660	23.600	250	177	8	182	425	71	312	4.23e-12	67.4
MS.gene30176.t1	AT5G13480	31.319	182	115	7	162	340	241	415	9.38e-12	67.8
MS.gene30176.t1	AT5G13480	27.391	230	148	10	186	408	137	354	1.74e-11	67.0
MS.gene30176.t1	AT5G67320	24.016	254	151	8	175	426	356	569	9.46e-12	67.8
MS.gene30176.t1	AT5G13480	31.319	182	115	7	162	340	218	392	9.97e-12	67.4
MS.gene30176.t1	AT5G13480	27.391	230	148	10	186	408	114	331	2.14e-11	66.6
MS.gene30176.t1	AT2G33340	26.042	192	134	5	238	426	236	422	1.15e-11	67.0
MS.gene30176.t1	AT2G33340	26.042	192	134	5	238	426	236	422	1.15e-11	67.0
MS.gene30176.t1	AT5G13480	31.319	182	115	7	162	340	218	392	1.15e-11	67.4
MS.gene30176.t1	AT5G13480	27.391	230	148	10	186	408	114	331	2.52e-11	66.2
MS.gene30176.t1	AT2G33340	26.042	192	134	5	238	426	236	422	1.22e-11	67.0
MS.gene30176.t1	AT2G41500	25.203	246	134	8	186	429	342	539	1.35e-11	67.0
MS.gene30176.t1	AT2G41500	25.203	246	134	8	186	429	342	539	1.35e-11	67.0
MS.gene30176.t1	AT5G13480	31.319	182	115	7	162	340	230	404	1.51e-11	67.0
MS.gene30176.t1	AT5G13480	27.391	230	148	10	186	408	126	343	2.96e-11	66.2
MS.gene30176.t1	AT1G04510	25.116	215	152	5	238	448	236	445	3.20e-11	65.9
MS.gene30176.t1	AT1G04510	25.116	215	152	5	238	448	236	445	3.43e-11	65.9
MS.gene30176.t1	AT4G02730	26.852	216	145	9	216	426	34	241	5.30e-11	64.3
MS.gene30176.t1	AT5G56130	23.786	206	113	5	263	426	16	219	7.45e-11	63.5

Find 132 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGATGAAACCTTGAGGTTT+TGG	0.193519	3.3:+43103196	MS.gene30176:CDS
TGACTATTACCTCAATTTAC+TGG	0.257532	3.3:+43102255	MS.gene30176:CDS
GTTGCCACTGTCCAACCATC+TGG	0.273358	3.3:-43103165	None:intergenic
TTACCTCAATTTACTGGATT+GGG	0.283267	3.3:+43102261	MS.gene30176:CDS
CTCCTTCCAGAGAAGCTTAC+AGG	0.301895	3.3:+43101682	MS.gene30176:CDS
TCAGACTTCTGAGAGGATAT+TGG	0.304579	3.3:+43102213	MS.gene30176:intron
AATGTTCTTGCAATAACATT+AGG	0.318136	3.3:+43102292	MS.gene30176:CDS
CCTGAAGAGGAAAACGTGTT+TGG	0.319867	3.3:-43101365	None:intergenic
AACGGCTCAACTCTTGCTTT+TGG	0.338376	3.3:-43103258	None:intergenic
GTTCTTGAAAGCAAGAATTC+TGG	0.338489	3.3:-43101739	None:intergenic
GGATTGAAATGGTCGGCTTC+TGG	0.343844	3.3:+43102757	MS.gene30176:CDS
ATGATAATCAACTTTACATC+TGG	0.350065	3.3:+43102803	MS.gene30176:CDS
GCGATGAAACCATCAAGTTT+TGG	0.364930	3.3:+43102941	MS.gene30176:CDS
CTGAAGAGGAAAACGTGTTT+GGG	0.370197	3.3:-43101364	None:intergenic
ATTACCTCAATTTACTGGAT+TGG	0.371678	3.3:+43102260	MS.gene30176:CDS
TTCACTCTAACAATGGCTTC+AGG	0.374235	3.3:+43101315	None:intergenic
TAGCTTCCTGTAAGCTTCTC+TGG	0.375997	3.3:-43101688	None:intergenic
TCTCATTCTTGTTCCACAAC+AGG	0.376647	3.3:-43103020	None:intergenic
AAATCCATCGCTGATCTATT+CGG	0.383791	3.3:-43101591	None:intergenic
GCTCCTGATGGCCGTCATAT+TGG	0.389395	3.3:+43102409	MS.gene30176:CDS
ATTTCAATCCGCAAACTTCT+CGG	0.394694	3.3:-43102744	None:intergenic
AATAATTCGCCGGGGCTTGA+TGG	0.396021	3.3:-43101835	None:intergenic
CCCTATCACATCTGTAAGTT+GGG	0.396604	3.3:+43102387	MS.gene30176:CDS
TCTGCCATCTTCACCATTGA+AGG	0.397901	3.3:-43103099	None:intergenic
CTCAGTTGACACTGGGTCTC+AGG	0.408962	3.3:+43102990	MS.gene30176:CDS
CAGAAGCTTTAAACATGAAC+AGG	0.411753	3.3:+43101715	MS.gene30176:CDS
TAGGAAACACATTGTATTTG+TGG	0.412949	3.3:+43102311	MS.gene30176:CDS
CAAGGGAATTTACTAGCTAC+TGG	0.417964	3.3:+43102907	MS.gene30176:CDS
GCCCTATCACATCTGTAAGT+TGG	0.420766	3.3:+43102386	MS.gene30176:CDS
GAGAAGTTTGCGGATTGAAA+TGG	0.425267	3.3:+43102746	MS.gene30176:CDS
CTCAGGTATGTTCCCTGTTG+TGG	0.426699	3.3:+43103007	MS.gene30176:CDS
TAATAGTCATCCACAATGTC+TGG	0.428065	3.3:-43102241	None:intergenic
CTTGCTTGGTGTCCTTTCCA+AGG	0.428743	3.3:+43102889	MS.gene30176:CDS
GTGGGTATTCCAAAACTTGA+TGG	0.428833	3.3:-43102950	None:intergenic
TAGGCAGCGAGTAGGGTCTT+TGG	0.428851	3.3:+43102618	MS.gene30176:CDS
GTCGGCTTCTGGTCTACAAT+TGG	0.444050	3.3:+43102768	MS.gene30176:CDS
GCCCAACTTACAGATGTGAT+AGG	0.450468	3.3:-43102388	None:intergenic
AGGAAACACATTGTATTTGT+GGG	0.452538	3.3:+43102312	MS.gene30176:CDS
CTTCAACAAGGTGGCTTCAC+AGG	0.460645	3.3:+43102836	MS.gene30176:CDS
CTCCGTAAGTGTTCCTGAAG+AGG	0.466289	3.3:-43101378	None:intergenic
TTGCTTGGTGTCCTTTCCAA+GGG	0.471233	3.3:+43102890	MS.gene30176:CDS
CACAGAATCAGATAACACTT+TGG	0.480456	3.3:+43103070	MS.gene30176:CDS
TCATGAGAGAAGAAATCAAC+TGG	0.484334	3.3:-43101777	None:intergenic
ACAACTCTGAAGTGCAGCTA+TGG	0.484474	3.3:+43102443	MS.gene30176:CDS
GGATACAGCTTCAGATAGAC+AGG	0.484963	3.3:+43102465	MS.gene30176:CDS
ATGCCAATATGACGGCCATC+AGG	0.485428	3.3:-43102412	None:intergenic
TTCCTCTTCAGGAACACTTA+CGG	0.486142	3.3:+43101376	MS.gene30176:CDS
CTAGCTACTGGCGGAGGCAG+TGG	0.487153	3.3:+43102919	MS.gene30176:CDS
CACTTCAAGAGTTCTTCATA+TGG	0.487383	3.3:+43103134	MS.gene30176:CDS
TTGATTATCATTCCCTCCAC+TGG	0.491219	3.3:-43102791	None:intergenic
TTGCCACTGTCCAACCATCT+GGG	0.492660	3.3:-43103164	None:intergenic
TGGAAGGAGAGCACACTTGT+GGG	0.493184	3.3:-43101668	None:intergenic
ACTTTGGAAATATCCTTCAA+TGG	0.495344	3.3:+43103086	MS.gene30176:CDS
GATGGCCGTCATATTGGCAT+TGG	0.495923	3.3:+43102415	MS.gene30176:CDS
ATATCCTTCAATGGTGAAGA+TGG	0.498891	3.3:+43103095	MS.gene30176:CDS
CGCACTACATGGTGACAGAA+GGG	0.504327	3.3:+43101622	MS.gene30176:CDS
CTGGAAGGAGAGCACACTTG+TGG	0.508404	3.3:-43101669	None:intergenic
AAGAAATCAACTGGCGGCGT+AGG	0.510142	3.3:-43101768	None:intergenic
GGGCATGACCGAGAAGTTTG+CGG	0.518071	3.3:+43102736	MS.gene30176:CDS
TGTTATTGCAAGAACATTGG+CGG	0.521220	3.3:-43102287	None:intergenic
TACCTCAATTTACTGGATTG+GGG	0.527786	3.3:+43102262	MS.gene30176:CDS
CTGCAGTTGAGAACTTTGAA+AGG	0.529944	3.3:+43102589	MS.gene30176:intron
GAACTTTGAAAGGTGGGCAT+AGG	0.530523	3.3:+43102599	MS.gene30176:CDS
AATAACCACATACTCACAAC+CGG	0.531617	3.3:+43102646	MS.gene30176:CDS
TAATGTTATTGCAAGAACAT+TGG	0.532142	3.3:-43102290	None:intergenic
TCCCTCCGGTTGTGAGTATG+TGG	0.533027	3.3:-43102651	None:intergenic
GCCCCGGCGAATTATTCCTC+AGG	0.533857	3.3:+43101842	MS.gene30176:CDS
CTCATTCTTGTTCCACAACA+GGG	0.537252	3.3:-43103019	None:intergenic
CTGCAGTAAAAGCTCTTGCT+TGG	0.539760	3.3:+43102875	MS.gene30176:CDS
ACACCTGAGGAATAATTCGC+CGG	0.542147	3.3:-43101845	None:intergenic
TGATAATCAACTTTACATCT+GGG	0.543770	3.3:+43102804	MS.gene30176:CDS
TTTCGATTATGCGCACTACA+TGG	0.544215	3.3:+43101611	MS.gene30176:CDS
TGCTTGAACTCAGTTGACAC+TGG	0.544299	3.3:+43102982	MS.gene30176:CDS
GACAGTGGCAACAGCTGCAG+CGG	0.545471	3.3:+43103176	MS.gene30176:CDS
AGGGAGCTACTCAGCTCTCA+TGG	0.545560	3.3:+43103042	MS.gene30176:CDS
AACGGTAACAAGTTCTGAAG+TGG	0.549127	3.3:-43102350	None:intergenic
TATTTGTGGGATGCATCAAA+CGG	0.551368	3.3:+43102325	MS.gene30176:CDS
AAATCAACTGGCGGCGTAGG+TGG	0.551699	3.3:-43101765	None:intergenic
CAACTCTGAAGTGCAGCTAT+GGG	0.552386	3.3:+43102444	MS.gene30176:CDS
CTCACAACCGGAGGGATGGA+TGG	0.555824	3.3:+43102658	MS.gene30176:CDS
CATATGGCGCAGAGCCCAGA+TGG	0.555918	3.3:+43103150	MS.gene30176:CDS
GTGGGCATAGGCAGCGAGTA+GGG	0.557831	3.3:+43102611	MS.gene30176:CDS
CACCTGAGGAATAATTCGCC+GGG	0.559324	3.3:-43101844	None:intergenic
AGTTGAGAACTTTGAAAGGT+GGG	0.559397	3.3:+43102593	MS.gene30176:intron
TGGCGCAGAGCCCAGATGGT+TGG	0.562028	3.3:+43103154	MS.gene30176:CDS
AGGGGCTAAAGCTAAAGGTA+AGG	0.567414	3.3:+43101641	MS.gene30176:CDS
GCTAGTAAATTCCCTTGGAA+AGG	0.568517	3.3:-43102901	None:intergenic
GTCTACAATTGGCCAGTGGA+GGG	0.570881	3.3:+43102779	MS.gene30176:CDS
GCGCACTACATGGTGACAGA+AGG	0.570973	3.3:+43101621	MS.gene30176:CDS
GTGAAGATGGCAGAACTCAA+TGG	0.571074	3.3:+43103108	MS.gene30176:CDS
CATACTCACAACCGGAGGGA+TGG	0.571933	3.3:+43102654	MS.gene30176:CDS
CAGTTGAGAACTTTGAAAGG+TGG	0.572101	3.3:+43102592	MS.gene30176:intron
GGTCTACAATTGGCCAGTGG+AGG	0.575013	3.3:+43102778	MS.gene30176:CDS
TTCCCCAATCCAGTAAATTG+AGG	0.576674	3.3:-43102264	None:intergenic
ACAGAAGGGGCTAAAGCTAA+AGG	0.578064	3.3:+43101636	MS.gene30176:CDS
TTCCTGTAAGCTTCTCTGGA+AGG	0.579286	3.3:-43101684	None:intergenic
TCTGTAAGTTGGGCTCCTGA+TGG	0.584539	3.3:+43102397	MS.gene30176:CDS
CCCAACTTACAGATGTGATA+GGG	0.585245	3.3:-43102387	None:intergenic
AATTCTACCATCCATCCCTC+CGG	0.585408	3.3:-43102665	None:intergenic
AAAAGCATTCCAAAACCTCA+AGG	0.587395	3.3:-43103205	None:intergenic
GGTGGGCATAGGCAGCGAGT+AGG	0.596714	3.3:+43102610	MS.gene30176:CDS
AGCGAGTAGGGTCTTTGGCA+TGG	0.601597	3.3:+43102623	MS.gene30176:CDS
GTTACCGTTGATGATGAAGA+TGG	0.607070	3.3:+43102364	MS.gene30176:CDS
ATTGGATGCTCCAGACATTG+TGG	0.612151	3.3:+43102231	MS.gene30176:CDS
AAAATAAAACCATCAAGCCC+CGG	0.612311	3.3:+43101826	MS.gene30176:CDS
TGTTGTGGAACAAGAATGAG+AGG	0.618210	3.3:+43103022	MS.gene30176:CDS
ATGCGGCTCAAATGTGAGAA+CGG	0.625587	3.3:-43103276	None:intergenic
AGGGCCATCTTCATCATCAA+CGG	0.626287	3.3:-43102368	None:intergenic
GCTTGAACTCAGTTGACACT+GGG	0.627877	3.3:+43102983	MS.gene30176:CDS
CAGTAGCTAGTAAATTCCCT+TGG	0.629888	3.3:-43102906	None:intergenic
AATTTACTAGCTACTGGCGG+AGG	0.633586	3.3:+43102913	MS.gene30176:CDS
TCAAGCCAATGCCAATATGA+CGG	0.636859	3.3:-43102420	None:intergenic
GAGCCCAGATGGTTGGACAG+TGG	0.641101	3.3:+43103161	MS.gene30176:CDS
TCTGGTCTACAATTGGCCAG+TGG	0.642557	3.3:+43102775	MS.gene30176:CDS
ACCACATACTCACAACCGGA+GGG	0.650856	3.3:+43102650	MS.gene30176:CDS
CATACCGAATAGATCAGCGA+TGG	0.652740	3.3:+43101587	MS.gene30176:CDS
AGTTTGCGGATTGAAATGGT+CGG	0.668119	3.3:+43102750	MS.gene30176:CDS
GTTCAAGCATGCACCTGTGT+GGG	0.668846	3.3:-43102968	None:intergenic
TTGTTTCTCAGACTTCTGAG+AGG	0.673720	3.3:+43102206	MS.gene30176:intron
GCACTACATGGTGACAGAAG+GGG	0.674164	3.3:+43101623	MS.gene30176:CDS
CTGCAGCGGATGAAACCTTG+AGG	0.678181	3.3:+43103190	MS.gene30176:CDS
AGTTCAAGCATGCACCTGTG+TGG	0.678713	3.3:-43102969	None:intergenic
ACATTGTTGAAACATACAGA+GGG	0.688874	3.3:+43102716	MS.gene30176:CDS
AACCACATACTCACAACCGG+AGG	0.695066	3.3:+43102649	MS.gene30176:CDS
TGAGAGAAGAAATCAACTGG+CGG	0.700989	3.3:-43101774	None:intergenic
CACATTGTTGAAACATACAG+AGG	0.702329	3.3:+43102715	MS.gene30176:CDS
GGGATAGAAGTACTTCAACA+AGG	0.702662	3.3:+43102824	MS.gene30176:CDS
GGGAATTTACTAGCTACTGG+CGG	0.715639	3.3:+43102910	MS.gene30176:CDS
ATGTTACTACTTATCGAATG+CGG	0.717524	3.3:-43103293	None:intergenic
GTTGTGGAACAAGAATGAGA+GGG	0.727155	3.3:+43103023	MS.gene30176:CDS
ACCTGAGGAATAATTCGCCG+GGG	0.733838	3.3:-43101843	None:intergenic
ATAGAAGTACTTCAACAAGG+TGG	0.753913	3.3:+43102827	MS.gene30176:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAGAAATTTTTTCATCATTA+GGG	+	chr3.3:43101422-43101441	MS.gene30176:intron	15.0%
!!!	CAAGTACAAAATTTTAAAAT+CGG	-	chr3.3:43101977-43101996	None:intergenic	15.0%
!!!	TAAGAAATTTTTTCATCATT+AGG	+	chr3.3:43101421-43101440	MS.gene30176:intron	15.0%
!!	AATATTAGAAAACTAGTAGT+AGG	-	chr3.3:43102554-43102573	None:intergenic	20.0%
!!!	ATAATAATGTATCGATTGAA+GGG	-	chr3.3:43102511-43102530	None:intergenic	20.0%
!	AATGTTCTTGCAATAACATT+AGG	+	chr3.3:43102292-43102311	MS.gene30176:CDS	25.0%
!	ATGATAATCAACTTTACATC+TGG	+	chr3.3:43102803-43102822	MS.gene30176:CDS	25.0%
!	TAATGTTATTGCAAGAACAT+TGG	-	chr3.3:43102293-43102312	None:intergenic	25.0%
!	TCAATCAATTAGCACAAAAT+TGG	-	chr3.3:43102182-43102201	None:intergenic	25.0%
!	TGATAATCAACTTTACATCT+GGG	+	chr3.3:43102804-43102823	MS.gene30176:CDS	25.0%
!!	ATTAGTTTCTGAAAATCAAG+TGG	+	chr3.3:43101997-43102016	MS.gene30176:intron	25.0%
!!	CACAAAATTGGAAAATGTTT+TGG	-	chr3.3:43102170-43102189	None:intergenic	25.0%
!!	GATTTGAGAATTTTGAACTT+CGG	+	chr3.3:43102105-43102124	MS.gene30176:intron	25.0%
!!	TTAGTTTCTGAAAATCAAGT+GGG	+	chr3.3:43101998-43102017	MS.gene30176:intron	25.0%
!!!	CATAATAATGTATCGATTGA+AGG	-	chr3.3:43102512-43102531	None:intergenic	25.0%
	ACATTGTTGAAACATACAGA+GGG	+	chr3.3:43102716-43102735	MS.gene30176:CDS	30.0%
	AGAAAACTAGTAGTAGGAAA+TGG	-	chr3.3:43102548-43102567	None:intergenic	30.0%
	AGGAAACACATTGTATTTGT+GGG	+	chr3.3:43102312-43102331	MS.gene30176:CDS	30.0%
	ATTACCTCAATTTACTGGAT+TGG	+	chr3.3:43102260-43102279	MS.gene30176:CDS	30.0%
	TAGGAAACACATTGTATTTG+TGG	+	chr3.3:43102311-43102330	MS.gene30176:CDS	30.0%
	TGACTATTACCTCAATTTAC+TGG	+	chr3.3:43102255-43102274	MS.gene30176:CDS	30.0%
	TTACCTCAATTTACTGGATT+GGG	+	chr3.3:43102261-43102280	MS.gene30176:CDS	30.0%
!	ACTTTGGAAATATCCTTCAA+TGG	+	chr3.3:43103086-43103105	MS.gene30176:CDS	30.0%
!	TGTTTTCGTAATCGTTTGTA+TGG	+	chr3.3:43102028-43102047	MS.gene30176:intron	30.0%
!!	AGACAGGTTTTATACTTATC+TGG	+	chr3.3:43102481-43102500	MS.gene30176:intron	30.0%
!!	TCTTATCTGAAAGTGTTGTA+TGG	+	chr3.3:43102138-43102157	MS.gene30176:intron	30.0%
!!	TCTTGTGCTAATTGATTTGT+TGG	+	chr3.3:43102076-43102095	MS.gene30176:intron	30.0%
	AAAATAAAACCATCAAGCCC+CGG	+	chr3.3:43101826-43101845	MS.gene30176:CDS	35.0%
	AAATCCATCGCTGATCTATT+CGG	-	chr3.3:43101594-43101613	None:intergenic	35.0%
	AATAACCACATACTCACAAC+CGG	+	chr3.3:43102646-43102665	MS.gene30176:CDS	35.0%
	ATATCCTTCAATGGTGAAGA+TGG	+	chr3.3:43103095-43103114	MS.gene30176:CDS	35.0%
	ATTTCAATCCGCAAACTTCT+CGG	-	chr3.3:43102747-43102766	None:intergenic	35.0%
	CACAGAATCAGATAACACTT+TGG	+	chr3.3:43103070-43103089	MS.gene30176:CDS	35.0%
	CACATTGTTGAAACATACAG+AGG	+	chr3.3:43102715-43102734	MS.gene30176:CDS	35.0%
	CACTTCAAGAGTTCTTCATA+TGG	+	chr3.3:43103134-43103153	MS.gene30176:CDS	35.0%
	CCATGTCATGATAACTAACT+AGG	+	chr3.3:43101477-43101496	MS.gene30176:intron	35.0%
	CCTAGTTAGTTATCATGACA+TGG	-	chr3.3:43101480-43101499	None:intergenic	35.0%
	GTTCTTGAAAGCAAGAATTC+TGG	-	chr3.3:43101742-43101761	None:intergenic	35.0%
	TAATAGTCATCCACAATGTC+TGG	-	chr3.3:43102244-43102263	None:intergenic	35.0%
	TACCTCAATTTACTGGATTG+GGG	+	chr3.3:43102262-43102281	MS.gene30176:CDS	35.0%
	TATTTGTGGGATGCATCAAA+CGG	+	chr3.3:43102325-43102344	MS.gene30176:CDS	35.0%
	TCATGAGAGAAGAAATCAAC+TGG	-	chr3.3:43101780-43101799	None:intergenic	35.0%
	TGTTATTGCAAGAACATTGG+CGG	-	chr3.3:43102290-43102309	None:intergenic	35.0%
!	AGTTGAGAACTTTGAAAGGT+GGG	+	chr3.3:43102593-43102612	MS.gene30176:intron	35.0%
!!	AAAACGTGTTTGGGTTTTGA+TGG	-	chr3.3:43101358-43101377	None:intergenic	35.0%
!!	AAAAGCATTCCAAAACCTCA+AGG	-	chr3.3:43103208-43103227	None:intergenic	35.0%
!!	ATAGAAGTACTTCAACAAGG+TGG	+	chr3.3:43102827-43102846	MS.gene30176:CDS	35.0%
!!	CAGAAGCTTTAAACATGAAC+AGG	+	chr3.3:43101715-43101734	MS.gene30176:CDS	35.0%
!!!	TTGAGGTTTTGGAATGCTTT+TGG	+	chr3.3:43103207-43103226	MS.gene30176:CDS	35.0%
	CAAGGGAATTTACTAGCTAC+TGG	+	chr3.3:43102907-43102926	MS.gene30176:CDS	40.0%
	CAGTAGCTAGTAAATTCCCT+TGG	-	chr3.3:43102909-43102928	None:intergenic	40.0%
	CCCAACTTACAGATGTGATA+GGG	-	chr3.3:43102390-43102409	None:intergenic	40.0%
	CCCTATCACATCTGTAAGTT+GGG	+	chr3.3:43102387-43102406	MS.gene30176:CDS	40.0%
	CTCATTCTTGTTCCACAACA+GGG	-	chr3.3:43103022-43103041	None:intergenic	40.0%
	CTGAAGAGGAAAACGTGTTT+GGG	-	chr3.3:43101367-43101386	None:intergenic	40.0%
	CTGCAGTTGAGAACTTTGAA+AGG	+	chr3.3:43102589-43102608	MS.gene30176:intron	40.0%
	GCTAGTAAATTCCCTTGGAA+AGG	-	chr3.3:43102904-43102923	None:intergenic	40.0%
	GTGGGTATTCCAAAACTTGA+TGG	-	chr3.3:43102953-43102972	None:intergenic	40.0%
	GTTGTGGAACAAGAATGAGA+GGG	+	chr3.3:43103023-43103042	MS.gene30176:CDS	40.0%
	TCAAGCCAATGCCAATATGA+CGG	-	chr3.3:43102423-43102442	None:intergenic	40.0%
	TCGGTATGAATCTATCCAAC+TGG	-	chr3.3:43101575-43101594	None:intergenic	40.0%
	TCTCATTCTTGTTCCACAAC+AGG	-	chr3.3:43103023-43103042	None:intergenic	40.0%
	TGAGAGAAGAAATCAACTGG+CGG	-	chr3.3:43101777-43101796	None:intergenic	40.0%
	TGTTGTGGAACAAGAATGAG+AGG	+	chr3.3:43103022-43103041	MS.gene30176:CDS	40.0%
	TTCCCCAATCCAGTAAATTG+AGG	-	chr3.3:43102267-43102286	None:intergenic	40.0%
	TTCCTCTTCAGGAACACTTA+CGG	+	chr3.3:43101376-43101395	MS.gene30176:CDS	40.0%
	TTGATTATCATTCCCTCCAC+TGG	-	chr3.3:43102794-43102813	None:intergenic	40.0%
	TTTCGATTATGCGCACTACA+TGG	+	chr3.3:43101611-43101630	MS.gene30176:CDS	40.0%
!	AGTTTGCGGATTGAAATGGT+CGG	+	chr3.3:43102750-43102769	MS.gene30176:CDS	40.0%
!	CAGTTGAGAACTTTGAAAGG+TGG	+	chr3.3:43102592-43102611	MS.gene30176:intron	40.0%
!	GAGAAGTTTGCGGATTGAAA+TGG	+	chr3.3:43102746-43102765	MS.gene30176:CDS	40.0%
!	GCGATGAAACCATCAAGTTT+TGG	+	chr3.3:43102941-43102960	MS.gene30176:CDS	40.0%
!	TCAGACTTCTGAGAGGATAT+TGG	+	chr3.3:43102213-43102232	MS.gene30176:intron	40.0%
!	TTGTTTCTCAGACTTCTGAG+AGG	+	chr3.3:43102206-43102225	MS.gene30176:intron	40.0%
!!	AACGGTAACAAGTTCTGAAG+TGG	-	chr3.3:43102353-43102372	None:intergenic	40.0%
!!	GGGATAGAAGTACTTCAACA+AGG	+	chr3.3:43102824-43102843	MS.gene30176:CDS	40.0%
!!	GTTACCGTTGATGATGAAGA+TGG	+	chr3.3:43102364-43102383	MS.gene30176:CDS	40.0%
!!	TTGACTTGTTTGTGACCAGT+TGG	+	chr3.3:43101557-43101576	MS.gene30176:intron	40.0%
!!!	AAGTTTTGGAATACCCACAC+AGG	+	chr3.3:43102955-43102974	MS.gene30176:CDS	40.0%
	AAGAAACACACACACACCTG+AGG	-	chr3.3:43101861-43101880	None:intergenic	45.0%
	AATTCTACCATCCATCCCTC+CGG	-	chr3.3:43102668-43102687	None:intergenic	45.0%
	AATTTACTAGCTACTGGCGG+AGG	+	chr3.3:43102913-43102932	MS.gene30176:CDS	45.0%
	ACACCTGAGGAATAATTCGC+CGG	-	chr3.3:43101848-43101867	None:intergenic	45.0%
	ACAGAAGGGGCTAAAGCTAA+AGG	+	chr3.3:43101636-43101655	MS.gene30176:CDS	45.0%
	AGGGCCATCTTCATCATCAA+CGG	-	chr3.3:43102371-43102390	None:intergenic	45.0%
	AGGGGCTAAAGCTAAAGGTA+AGG	+	chr3.3:43101641-43101660	MS.gene30176:CDS	45.0%
	ATGCGGCTCAAATGTGAGAA+CGG	-	chr3.3:43103279-43103298	None:intergenic	45.0%
	ATTGGATGCTCCAGACATTG+TGG	+	chr3.3:43102231-43102250	MS.gene30176:CDS	45.0%
	CATACCGAATAGATCAGCGA+TGG	+	chr3.3:43101587-43101606	MS.gene30176:CDS	45.0%
	CCTGAAGAGGAAAACGTGTT+TGG	-	chr3.3:43101368-43101387	None:intergenic	45.0%
	CTGCAGTAAAAGCTCTTGCT+TGG	+	chr3.3:43102875-43102894	MS.gene30176:CDS	45.0%
	GCCCAACTTACAGATGTGAT+AGG	-	chr3.3:43102391-43102410	None:intergenic	45.0%
	GCCCTATCACATCTGTAAGT+TGG	+	chr3.3:43102386-43102405	MS.gene30176:CDS	45.0%
	GCTTGAACTCAGTTGACACT+GGG	+	chr3.3:43102983-43103002	MS.gene30176:CDS	45.0%
	GGATACAGCTTCAGATAGAC+AGG	+	chr3.3:43102465-43102484	MS.gene30176:CDS	45.0%
	GGGAATTTACTAGCTACTGG+CGG	+	chr3.3:43102910-43102929	MS.gene30176:CDS	45.0%
	GTGAAGATGGCAGAACTCAA+TGG	+	chr3.3:43103108-43103127	MS.gene30176:CDS	45.0%
	TAGCTTCCTGTAAGCTTCTC+TGG	-	chr3.3:43101691-43101710	None:intergenic	45.0%
	TCTGCCATCTTCACCATTGA+AGG	-	chr3.3:43103102-43103121	None:intergenic	45.0%
	TGCTTGAACTCAGTTGACAC+TGG	+	chr3.3:43102982-43103001	MS.gene30176:CDS	45.0%
	TTCCTGTAAGCTTCTCTGGA+AGG	-	chr3.3:43101687-43101706	None:intergenic	45.0%
!	AACGGCTCAACTCTTGCTTT+TGG	-	chr3.3:43103261-43103280	None:intergenic	45.0%
!	CCAAACACGTTTTCCTCTTC+AGG	+	chr3.3:43101365-43101384	MS.gene30176:CDS	45.0%
!	CGGATGAAACCTTGAGGTTT+TGG	+	chr3.3:43103196-43103215	MS.gene30176:CDS	45.0%
!	GAACTTTGAAAGGTGGGCAT+AGG	+	chr3.3:43102599-43102618	MS.gene30176:CDS	45.0%
!!	ACAACTCTGAAGTGCAGCTA+TGG	+	chr3.3:43102443-43102462	MS.gene30176:CDS	45.0%
!!	CAACTCTGAAGTGCAGCTAT+GGG	+	chr3.3:43102444-43102463	MS.gene30176:CDS	45.0%
!!	GCAGCTTTTGTTGCTACTTC+TGG	-	chr3.3:43103237-43103256	None:intergenic	45.0%
!!	TTGCTTGGTGTCCTTTCCAA+GGG	+	chr3.3:43102890-43102909	MS.gene30176:CDS	45.0%
!!!	TTATTTTATCATTATTAATT+TGG	+	chr3.3:43101904-43101923	MS.gene30176:intron	5.0%
	AACCACATACTCACAACCGG+AGG	+	chr3.3:43102649-43102668	MS.gene30176:CDS	50.0%
	AAGAAATCAACTGGCGGCGT+AGG	-	chr3.3:43101771-43101790	None:intergenic	50.0%
	AATAATTCGCCGGGGCTTGA+TGG	-	chr3.3:43101838-43101857	None:intergenic	50.0%
	ACCACATACTCACAACCGGA+GGG	+	chr3.3:43102650-43102669	MS.gene30176:CDS	50.0%
	ACCTGAGGAATAATTCGCCG+GGG	-	chr3.3:43101846-43101865	None:intergenic	50.0%
	AGTTCAAGCATGCACCTGTG+TGG	-	chr3.3:43102972-43102991	None:intergenic	50.0%
	ATGCCAATATGACGGCCATC+AGG	-	chr3.3:43102415-43102434	None:intergenic	50.0%
	CACCTGAGGAATAATTCGCC+GGG	-	chr3.3:43101847-43101866	None:intergenic	50.0%
	CGCACTACATGGTGACAGAA+GGG	+	chr3.3:43101622-43101641	MS.gene30176:CDS	50.0%
	CTCAGGTATGTTCCCTGTTG+TGG	+	chr3.3:43103007-43103026	MS.gene30176:CDS	50.0%
	CTCCGTAAGTGTTCCTGAAG+AGG	-	chr3.3:43101381-43101400	None:intergenic	50.0%
	CTCCTTCCAGAGAAGCTTAC+AGG	+	chr3.3:43101682-43101701	MS.gene30176:CDS	50.0%
	CTTCAACAAGGTGGCTTCAC+AGG	+	chr3.3:43102836-43102855	MS.gene30176:CDS	50.0%
	GATGGCCGTCATATTGGCAT+TGG	+	chr3.3:43102415-43102434	MS.gene30176:CDS	50.0%
	GCACTACATGGTGACAGAAG+GGG	+	chr3.3:43101623-43101642	MS.gene30176:CDS	50.0%
	GTCTACAATTGGCCAGTGGA+GGG	+	chr3.3:43102779-43102798	MS.gene30176:CDS	50.0%
	GTTCAAGCATGCACCTGTGT+GGG	-	chr3.3:43102971-43102990	None:intergenic	50.0%
	TCTGGTCTACAATTGGCCAG+TGG	+	chr3.3:43102775-43102794	MS.gene30176:CDS	50.0%
	TTGCCACTGTCCAACCATCT+GGG	-	chr3.3:43103167-43103186	None:intergenic	50.0%
!	GGATTGAAATGGTCGGCTTC+TGG	+	chr3.3:43102757-43102776	MS.gene30176:CDS	50.0%
!	GTCGGCTTCTGGTCTACAAT+TGG	+	chr3.3:43102768-43102787	MS.gene30176:CDS	50.0%
!	TCTGTAAGTTGGGCTCCTGA+TGG	+	chr3.3:43102397-43102416	MS.gene30176:CDS	50.0%
!	TGGAAGGAGAGCACACTTGT+GGG	-	chr3.3:43101671-43101690	None:intergenic	50.0%
!!	CTTGCTTGGTGTCCTTTCCA+AGG	+	chr3.3:43102889-43102908	MS.gene30176:CDS	50.0%
	AAATCAACTGGCGGCGTAGG+TGG	-	chr3.3:43101768-43101787	None:intergenic	55.0%
	AGCGAGTAGGGTCTTTGGCA+TGG	+	chr3.3:43102623-43102642	MS.gene30176:CDS	55.0%
	AGGGAGCTACTCAGCTCTCA+TGG	+	chr3.3:43103042-43103061	MS.gene30176:CDS	55.0%
	CATACTCACAACCGGAGGGA+TGG	+	chr3.3:43102654-43102673	MS.gene30176:CDS	55.0%
	CTCAGTTGACACTGGGTCTC+AGG	+	chr3.3:43102990-43103009	MS.gene30176:CDS	55.0%
	CTGCAGCGGATGAAACCTTG+AGG	+	chr3.3:43103190-43103209	MS.gene30176:CDS	55.0%
	GCGCACTACATGGTGACAGA+AGG	+	chr3.3:43101621-43101640	MS.gene30176:CDS	55.0%
	GCTCCTGATGGCCGTCATAT+TGG	+	chr3.3:43102409-43102428	MS.gene30176:CDS	55.0%
	GGGCATGACCGAGAAGTTTG+CGG	+	chr3.3:43102736-43102755	MS.gene30176:CDS	55.0%
	GGTCTACAATTGGCCAGTGG+AGG	+	chr3.3:43102778-43102797	MS.gene30176:CDS	55.0%
	GTTGCCACTGTCCAACCATC+TGG	-	chr3.3:43103168-43103187	None:intergenic	55.0%
	TAGGCAGCGAGTAGGGTCTT+TGG	+	chr3.3:43102618-43102637	MS.gene30176:CDS	55.0%
	TCCCTCCGGTTGTGAGTATG+TGG	-	chr3.3:43102654-43102673	None:intergenic	55.0%
!	CTGGAAGGAGAGCACACTTG+TGG	-	chr3.3:43101672-43101691	None:intergenic	55.0%
	CATATGGCGCAGAGCCCAGA+TGG	+	chr3.3:43103150-43103169	MS.gene30176:CDS	60.0%
	CTCACAACCGGAGGGATGGA+TGG	+	chr3.3:43102658-43102677	MS.gene30176:CDS	60.0%
	GACAGTGGCAACAGCTGCAG+CGG	+	chr3.3:43103176-43103195	MS.gene30176:CDS	60.0%
	GAGCCCAGATGGTTGGACAG+TGG	+	chr3.3:43103161-43103180	MS.gene30176:CDS	60.0%
	GCCCCGGCGAATTATTCCTC+AGG	+	chr3.3:43101842-43101861	MS.gene30176:CDS	60.0%
	GTGGGCATAGGCAGCGAGTA+GGG	+	chr3.3:43102611-43102630	MS.gene30176:CDS	60.0%
	CTAGCTACTGGCGGAGGCAG+TGG	+	chr3.3:43102919-43102938	MS.gene30176:CDS	65.0%
	GGTGGGCATAGGCAGCGAGT+AGG	+	chr3.3:43102610-43102629	MS.gene30176:CDS	65.0%
	TGGCGCAGAGCCCAGATGGT+TGG	+	chr3.3:43103154-43103173	MS.gene30176:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	43101327	43103305	43101327	ID=MS.gene30176
chr3.3	mRNA	43101327	43103305	43101327	ID=MS.gene30176.t1;Parent=MS.gene30176
chr3.3	exon	43101327	43101419	43101327	ID=MS.gene30176.t1.exon1;Parent=MS.gene30176.t1
chr3.3	CDS	43101327	43101419	43101327	ID=cds.MS.gene30176.t1;Parent=MS.gene30176.t1
chr3.3	exon	43101576	43101863	43101576	ID=MS.gene30176.t1.exon2;Parent=MS.gene30176.t1
chr3.3	CDS	43101576	43101863	43101576	ID=cds.MS.gene30176.t1;Parent=MS.gene30176.t1
chr3.3	exon	43102217	43102486	43102217	ID=MS.gene30176.t1.exon3;Parent=MS.gene30176.t1
chr3.3	CDS	43102217	43102486	43102217	ID=cds.MS.gene30176.t1;Parent=MS.gene30176.t1
chr3.3	exon	43102595	43103305	43102595	ID=MS.gene30176.t1.exon4;Parent=MS.gene30176.t1
chr3.3	CDS	43102595	43103305	43102595	ID=cds.MS.gene30176.t1;Parent=MS.gene30176.t1

Gene Sequence

>MS.gene30176

ATGGCTTCAGGTTCTTTGAGTTCTTCTGCCATCAAAACCCAAACACGTTTTCCTCTTCAGGAACACTTACGGAGAAAGACTTCAAATGAAAATGTAAGAAATTTTTTCATCATTAGGGTTTCAAACTTTCAATGTAATTCTGTTTTGAAACCATGTCATGATAACTAACTAGGTACTTTATTGTGAGAATTTCTTTATAAAAATTAAGTAGTTTATTAATTATTTGATGTTTGACTTGTTTGTGACCAGTTGGATAGATTCATACCGAATAGATCAGCGATGGATTTCGATTATGCGCACTACATGGTGACAGAAGGGGCTAAAGCTAAAGGTAAGGAAAACCCACAAGTGTGCTCTCCTTCCAGAGAAGCTTACAGGAAGCTACTTGCAGAAGCTTTAAACATGAACAGGACCAGAATTCTTGCTTTCAAGAACAAGCCACCTACGCCGCCAGTTGATTTCTTCTCTCATGATATCATTACTTCATCAACTCTAAAACAAAATAAAACCATCAAGCCCCGGCGAATTATTCCTCAGGTGTGTGTGTGTTTCTTAATGATATCTATTCATATTTCATTTATTTTATCATTATTAATTTGGTTGCATGAACTCTAGTTTAAATGAACTCTAGTTTAAACAGCAAAAGTCCGATTTTAAAATTTTGTACTTGATTAGTTTCTGAAAATCAAGTGGGTCTGAGTTGTTTTCGTAATCGTTTGTATGGTGTGTGTAATCACAATAGTTTGCAATCTTGTGCTAATTGATTTGTTGGTTGTCTGATTTGAGAATTTTGAACTTCGGTTTTCTTAATTCTTATCTGAAAGTGTTGTATGGTGTAAACCAAAACATTTTCCAATTTTGTGCTAATTGATTGATATGTTGTTTCTCAGACTTCTGAGAGGATATTGGATGCTCCAGACATTGTGGATGACTATTACCTCAATTTACTGGATTGGGGAACCGCCAATGTTCTTGCAATAACATTAGGAAACACATTGTATTTGTGGGATGCATCAAACGGTTCCACTTCAGAACTTGTTACCGTTGATGATGAAGATGGCCCTATCACATCTGTAAGTTGGGCTCCTGATGGCCGTCATATTGGCATTGGCTTGAACAACTCTGAAGTGCAGCTATGGGATACAGCTTCAGATAGACAGGTTTTATACTTATCTGGTTTACCCTTCAATCGATACATTATTATGAGCTATTCAGTATCCATTTCCTACTACTAGTTTTCTAATATTATTCATGTTATGTTTCTGCAGTTGAGAACTTTGAAAGGTGGGCATAGGCAGCGAGTAGGGTCTTTGGCATGGAATAACCACATACTCACAACCGGAGGGATGGATGGTAGAATTATCAATAATGATGTAAGAATTAGAGCACACATTGTTGAAACATACAGAGGGCATGACCGAGAAGTTTGCGGATTGAAATGGTCGGCTTCTGGTCTACAATTGGCCAGTGGAGGGAATGATAATCAACTTTACATCTGGGATAGAAGTACTTCAACAAGGTGGCTTCACAGGCTTGAAGATCATACATCTGCAGTAAAAGCTCTTGCTTGGTGTCCTTTCCAAGGGAATTTACTAGCTACTGGCGGAGGCAGTGGCGATGAAACCATCAAGTTTTGGAATACCCACACAGGTGCATGCTTGAACTCAGTTGACACTGGGTCTCAGGTATGTTCCCTGTTGTGGAACAAGAATGAGAGGGAGCTACTCAGCTCTCATGGTTTTACACAGAATCAGATAACACTTTGGAAATATCCTTCAATGGTGAAGATGGCAGAACTCAATGGTCACACTTCAAGAGTTCTTCATATGGCGCAGAGCCCAGATGGTTGGACAGTGGCAACAGCTGCAGCGGATGAAACCTTGAGGTTTTGGAATGCTTTTGGCACTCCAGAAGTAGCAACAAAAGCTGCACCAAAAGCAAGAGTTGAGCCGTTCTCACATTTGAGCCGCATTCGATAA

Protein sequence

>MS.gene30176.t1

MASGSLSSSAIKTQTRFPLQEHLRRKTSNENLDRFIPNRSAMDFDYAHYMVTEGAKAKGKENPQVCSPSREAYRKLLAEALNMNRTRILAFKNKPPTPPVDFFSHDIITSSTLKQNKTIKPRRIIPQTSERILDAPDIVDDYYLNLLDWGTANVLAITLGNTLYLWDASNGSTSELVTVDDEDGPITSVSWAPDGRHIGIGLNNSEVQLWDTASDRQLRTLKGGHRQRVGSLAWNNHILTTGGMDGRIINNDVRIRAHIVETYRGHDREVCGLKWSASGLQLASGGNDNQLYIWDRSTSTRWLHRLEDHTSAVKALAWCPFQGNLLATGGGSGDETIKFWNTHTGACLNSVDTGSQVCSLLWNKNERELLSSHGFTQNQITLWKYPSMVKMAELNGHTSRVLHMAQSPDGWTVATAAADETLRFWNAFGTPEVATKAAPKARVEPFSHLSRIR