AlfalfaGEDB | MS.gene027075

M. sativa cultivar XinJiangDaYe / MS.gene027075

KEGG: K03363
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00400.35 PF12894.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027075.t1	XP_003600092.1	94.2	428	19	2	1	422	32	459	4.50E-240	840.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027075.t1	Q9SZA4	77.0	427	90	3	1	422	34	457	2.6e-196	686.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027075.t1	G7IY01	94.2	428	19	2	1	422	32	459	3.2e-240	840.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene36842	MS.gene027075	PPI
MS.gene027075	MS.gene37226	PPI
MS.gene027075	MS.gene049021	PPI
MS.gene066046	MS.gene027075	PPI
MS.gene027075	MS.gene043742	PPI
MS.gene027075	MS.gene027441	PPI
MS.gene41291	MS.gene027075	PPI
MS.gene027075	MS.gene022502	PPI
MS.gene027075	MS.gene050457	PPI
MS.gene027075	MS.gene66343	PPI
MS.gene027075	MS.gene99973	PPI
MS.gene027075	MS.gene84466	PPI
MS.gene027075	MS.gene008833	PPI
MS.gene027075	MS.gene064435	PPI
MS.gene030294	MS.gene027075	PPI
MS.gene91851	MS.gene027075	PPI
MS.gene29768	MS.gene027075	PPI
MS.gene027075	MS.gene48655	PPI
MS.gene039682	MS.gene027075	PPI
MS.gene027075	MS.gene81663	PPI
MS.gene042783	MS.gene027075	PPI
MS.gene066467	MS.gene027075	PPI
MS.gene027075	MS.gene027073	PPI
MS.gene82020	MS.gene027075	PPI
MS.gene21734	MS.gene027075	PPI
MS.gene33377	MS.gene027075	PPI
MS.gene71204	MS.gene027075	PPI
MS.gene027075	MS.gene037184	PPI
MS.gene064035	MS.gene027075	PPI
MS.gene77885	MS.gene027075	PPI
MS.gene027075	MS.gene067705	PPI
MS.gene027075	MS.gene038519	PPI
MS.gene26672	MS.gene027075	PPI
MS.gene97379	MS.gene027075	PPI
MS.gene027075	MS.gene045133	PPI
MS.gene99554	MS.gene027075	PPI
MS.gene90626	MS.gene027075	PPI
MS.gene027075	MS.gene20160	PPI
MS.gene42253	MS.gene027075	PPI
MS.gene058128	MS.gene027075	PPI
MS.gene011448	MS.gene027075	PPI
MS.gene066501	MS.gene027075	PPI
MS.gene25244	MS.gene027075	PPI
MS.gene027075	MS.gene30174	PPI
MS.gene027075	MS.gene26021	PPI
MS.gene63085	MS.gene027075	PPI
MS.gene056455	MS.gene027075	PPI
MS.gene027075	MS.gene050456	PPI
MS.gene89986	MS.gene027075	PPI
MS.gene027075	MS.gene51258	PPI
MS.gene064762	MS.gene027075	PPI
MS.gene027075	MS.gene053363	PPI
MS.gene027075	MS.gene91775	PPI
MS.gene049022	MS.gene027075	PPI
MS.gene35613	MS.gene027075	PPI
MS.gene69965	MS.gene027075	PPI
MS.gene027075	MS.gene32726	PPI
MS.gene027075	MS.gene050270	PPI
MS.gene82740	MS.gene027075	PPI
MS.gene027075	MS.gene052956	PPI
MS.gene027075	MS.gene81660	PPI
MS.gene30172	MS.gene027075	PPI
MS.gene067322	MS.gene027075	PPI
MS.gene027075	MS.gene004844	PPI
MS.gene027075	MS.gene99588	PPI
MS.gene23852	MS.gene027075	PPI
MS.gene69966	MS.gene027075	PPI
MS.gene97943	MS.gene027075	PPI
MS.gene071113	MS.gene027075	PPI
MS.gene027071	MS.gene027075	PPI
MS.gene027075	MS.gene27223	PPI
MS.gene67157	MS.gene027075	PPI
MS.gene69396	MS.gene027075	PPI
MS.gene012203	MS.gene027075	PPI
MS.gene022109	MS.gene027075	PPI
MS.gene34651	MS.gene027075	PPI
MS.gene027075	MS.gene014419	PPI
MS.gene027075	MS.gene26019	PPI
MS.gene010910	MS.gene027075	PPI
MS.gene027075	MS.gene050272	PPI
MS.gene027075	MS.gene98540	PPI
MS.gene025229	MS.gene027075	PPI
MS.gene027075	MS.gene62819	PPI
MS.gene063222	MS.gene027075	PPI
MS.gene25515	MS.gene027075	PPI
MS.gene060495	MS.gene027075	PPI
MS.gene07706	MS.gene027075	PPI
MS.gene027074	MS.gene027075	PPI
MS.gene069995	MS.gene027075	PPI
MS.gene027075	MS.gene65775	PPI
MS.gene027075	MS.gene32723	PPI
MS.gene027075	MS.gene067321	PPI
MS.gene66342	MS.gene027075	PPI
MS.gene027075	MS.gene29842	PPI
MS.gene027075	MS.gene55883	PPI
MS.gene027075	MS.gene033401	PPI
MS.gene69687	MS.gene027075	PPI
MS.gene027075	MS.gene75493	PPI
MS.gene027075	MS.gene068273	PPI
MS.gene23811	MS.gene027075	PPI
MS.gene23810	MS.gene027075	PPI
MS.gene027075	MS.gene71122	PPI
MS.gene46611	MS.gene027075	PPI
MS.gene027075	MS.gene06177	PPI
MS.gene027075	MS.gene51278	PPI
MS.gene51335	MS.gene027075	PPI
MS.gene82021	MS.gene027075	PPI
MS.gene024753	MS.gene027075	PPI
MS.gene81658	MS.gene027075	PPI
MS.gene84465	MS.gene027075	PPI
MS.gene78281	MS.gene027075	PPI
MS.gene55882	MS.gene027075	PPI
MS.gene027075	MS.gene85534	PPI
MS.gene037950	MS.gene027075	PPI
MS.gene027075	MS.gene014321	PPI
MS.gene027075	MS.gene51262	PPI
MS.gene027075	MS.gene75059	PPI
MS.gene027075	MS.gene064862	PPI
MS.gene027075	MS.gene24951	PPI
MS.gene027075	MS.gene23881	PPI
MS.gene068249	MS.gene027075	PPI
MS.gene027075	MS.gene06176	PPI
MS.gene027075	MS.gene014931	PPI
MS.gene060496	MS.gene027075	PPI
MS.gene027075	MS.gene062662	PPI
MS.gene027075	MS.gene21888	PPI
MS.gene64441	MS.gene027075	PPI
MS.gene24806	MS.gene027075	PPI
MS.gene52355	MS.gene027075	PPI
MS.gene30176	MS.gene027075	PPI
MS.gene027075	MS.gene066500	PPI
MS.gene027075	MS.gene30177	PPI
MS.gene027075	MS.gene70008	PPI
MS.gene066464	MS.gene027075	PPI
MS.gene20161	MS.gene027075	PPI
MS.gene027075	MS.gene80163	PPI
MS.gene24922	MS.gene027075	PPI
MS.gene027073	MS.gene027075	PPI
MS.gene33406	MS.gene027075	PPI
MS.gene010912	MS.gene027075	PPI
MS.gene027075	MS.gene045937	PPI
MS.gene027075	MS.gene027072	PPI
MS.gene027075	MS.gene65463	PPI
MS.gene34712	MS.gene027075	PPI
MS.gene92674	MS.gene027075	PPI
MS.gene027075	MS.gene052188	PPI
MS.gene027075	MS.gene87218	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027075.t1	MTR_3g051690	94.159	428	19	2	1	422	32	459	0.0	840
MS.gene027075.t1	MTR_5g008010	90.164	427	33	4	1	422	32	454	0.0	795
MS.gene027075.t1	MTR_7g106870	74.592	429	98	6	1	422	33	457	0.0	667
MS.gene027075.t1	MTR_7g451830	67.599	429	115	8	2	422	19	431	0.0	568
MS.gene027075.t1	MTR_7g047640	67.599	429	115	8	2	422	19	431	0.0	568
MS.gene027075.t1	MTR_7g057540	64.884	430	125	8	1	422	15	426	0.0	533
MS.gene027075.t1	MTR_7g055590	64.802	429	125	8	1	421	15	425	0.0	531
MS.gene027075.t1	MTR_7g053100	62.005	429	139	7	1	422	25	436	0.0	517
MS.gene027075.t1	MTR_7g034625	70.219	319	88	2	97	408	13	331	9.02e-166	469
MS.gene027075.t1	MTR_2g100540	50.580	431	158	12	2	422	12	397	3.13e-127	373
MS.gene027075.t1	MTR_2g100460	56.560	343	135	5	80	420	52	382	2.48e-126	370
MS.gene027075.t1	MTR_2g060710	44.282	411	207	8	2	398	26	428	2.02e-119	355
MS.gene027075.t1	MTR_3g067940	44.286	420	212	7	2	409	57	466	4.33e-116	348
MS.gene027075.t1	MTR_4g102510	43.198	419	206	7	2	401	51	456	1.15e-110	333
MS.gene027075.t1	MTR_4g415070	52.941	68	32	0	347	414	57	124	1.04e-16	80.9
MS.gene027075.t1	MTR_1g054300	26.250	320	175	11	147	419	114	419	1.71e-15	78.6
MS.gene027075.t1	MTR_1g054300	26.119	268	170	11	134	394	235	481	1.99e-14	75.5
MS.gene027075.t1	MTR_2g012630	27.397	219	148	8	127	342	35	245	2.78e-15	78.6
MS.gene027075.t1	MTR_2g012630	27.397	219	148	8	127	342	35	245	3.07e-15	78.6
MS.gene027075.t1	MTR_6g004040	28.326	233	153	10	113	339	19	243	6.04e-15	77.4
MS.gene027075.t1	MTR_5g016370	23.693	287	163	8	116	394	312	550	1.26e-14	76.3
MS.gene027075.t1	MTR_5g016350	22.997	287	179	7	110	395	230	475	5.99e-13	70.9
MS.gene027075.t1	MTR_5g016350	22.997	287	179	7	110	395	294	539	6.26e-13	70.9
MS.gene027075.t1	MTR_4g095058	24.752	303	192	13	120	395	241	534	5.72e-12	67.8
MS.gene027075.t1	MTR_4g095058	24.382	283	168	11	116	395	293	532	5.96e-12	67.8
MS.gene027075.t1	MTR_3g106080	24.351	308	191	10	128	403	326	623	9.51e-12	67.4
MS.gene027075.t1	MTR_7g086360	23.415	205	150	4	193	395	53	252	2.44e-11	65.1
MS.gene027075.t1	MTR_1g049000	39.362	94	47	3	173	257	8	100	4.71e-11	62.0
MS.gene027075.t1	MTR_4g119620	22.581	248	177	7	155	394	81	321	5.12e-11	63.9
MS.gene027075.t1	MTR_5g022690	27.011	174	120	5	139	310	266	434	8.58e-11	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027075.t1	AT4G33270	77.049	427	90	4	1	422	34	457	0.0	685
MS.gene027075.t1	AT4G33260	76.815	427	91	4	1	422	24	447	0.0	668
MS.gene027075.t1	AT4G33260	75.122	410	94	4	1	405	24	430	0.0	635
MS.gene027075.t1	AT5G27570	68.765	429	119	6	1	422	30	450	0.0	590
MS.gene027075.t1	AT5G27080	67.366	429	125	6	1	422	22	442	0.0	583
MS.gene027075.t1	AT5G26900	66.822	428	128	5	1	422	25	444	0.0	572
MS.gene027075.t1	AT5G27945	61.725	371	122	5	68	422	62	428	2.25e-156	449
MS.gene027075.t1	AT4G22910	42.822	411	216	7	2	401	62	464	8.99e-113	339
MS.gene027075.t1	AT4G11920	42.118	425	212	8	2	407	53	462	1.83e-110	333
MS.gene027075.t1	AT5G13840	51.475	305	145	3	100	404	164	465	2.88e-110	333
MS.gene027075.t1	AT5G13840	51.475	305	145	3	100	404	164	465	2.88e-110	333
MS.gene027075.t1	AT1G11160	28.155	206	131	7	119	313	16	215	4.98e-16	80.9
MS.gene027075.t1	AT1G11160	28.155	206	131	7	119	313	16	215	5.38e-16	80.9
MS.gene027075.t1	AT1G11160	28.155	206	131	7	119	313	16	215	5.48e-16	80.9
MS.gene027075.t1	AT5G08390	29.060	234	136	10	109	339	37	243	1.12e-15	79.7
MS.gene027075.t1	AT1G11160	30.247	162	105	6	154	313	18	173	5.82e-15	77.8
MS.gene027075.t1	AT1G61210	26.339	224	142	8	111	319	6	221	1.55e-14	76.3
MS.gene027075.t1	AT1G61210	26.126	222	145	8	111	319	6	221	1.80e-14	76.3
MS.gene027075.t1	AT1G61210	26.339	224	142	8	111	319	6	221	1.85e-14	76.3
MS.gene027075.t1	AT5G52820	28.226	248	154	12	155	396	244	473	3.81e-14	74.3
MS.gene027075.t1	AT5G23430	28.774	212	138	9	113	319	19	222	1.53e-13	73.2
MS.gene027075.t1	AT5G23430	28.774	212	138	9	113	319	19	222	1.76e-13	73.2
MS.gene027075.t1	AT5G23430	28.774	212	138	9	113	319	19	222	1.76e-13	73.2
MS.gene027075.t1	AT5G25150	25.749	334	209	12	104	409	305	627	4.36e-13	71.6
MS.gene027075.t1	AT5G25150	25.749	334	209	12	104	409	305	627	4.58e-13	71.6
MS.gene027075.t1	AT2G32700	25.610	246	163	11	155	394	553	784	6.06e-13	71.2
MS.gene027075.t1	AT2G32700	25.610	246	163	11	155	394	574	805	6.47e-13	71.2
MS.gene027075.t1	AT2G32700	25.610	246	163	11	155	394	555	786	6.57e-13	71.2
MS.gene027075.t1	AT2G32700	25.610	246	163	11	155	394	555	786	6.57e-13	71.2
MS.gene027075.t1	AT2G32700	25.610	246	163	11	155	394	555	786	6.57e-13	71.2
MS.gene027075.t1	AT2G32700	25.610	246	163	11	155	394	555	786	6.57e-13	71.2
MS.gene027075.t1	AT2G32700	25.610	246	163	11	155	394	555	786	6.57e-13	71.2
MS.gene027075.t1	AT5G67320	25.000	284	165	12	116	395	330	569	2.19e-12	69.3
MS.gene027075.t1	AT3G49660	23.600	250	177	8	151	394	71	312	4.31e-12	67.4
MS.gene027075.t1	AT5G13480	31.319	182	115	7	131	309	241	415	1.17e-11	67.0
MS.gene027075.t1	AT5G13480	27.391	230	148	10	155	377	137	354	1.86e-11	66.6
MS.gene027075.t1	AT5G13480	31.319	182	115	7	131	309	218	392	1.19e-11	67.0
MS.gene027075.t1	AT5G13480	27.391	230	148	10	155	377	114	331	2.09e-11	66.2
MS.gene027075.t1	AT2G33340	26.042	192	134	5	207	395	236	422	1.25e-11	67.0
MS.gene027075.t1	AT2G33340	26.042	192	134	5	207	395	236	422	1.25e-11	67.0
MS.gene027075.t1	AT2G41500	25.203	246	134	8	155	398	342	539	1.37e-11	66.6
MS.gene027075.t1	AT2G41500	25.203	246	134	8	155	398	342	539	1.37e-11	66.6
MS.gene027075.t1	AT2G33340	26.042	192	134	5	207	395	236	422	1.44e-11	66.6
MS.gene027075.t1	AT5G13480	31.319	182	115	7	131	309	218	392	1.49e-11	66.6
MS.gene027075.t1	AT5G13480	27.391	230	148	10	155	377	114	331	2.46e-11	66.2
MS.gene027075.t1	AT5G13480	31.319	182	115	7	131	309	230	404	1.77e-11	66.6
MS.gene027075.t1	AT5G13480	27.391	230	148	10	155	377	126	343	2.72e-11	65.9
MS.gene027075.t1	AT1G04510	25.116	215	152	5	207	417	236	445	3.12e-11	65.5
MS.gene027075.t1	AT1G04510	25.116	215	152	5	207	417	236	445	3.32e-11	65.5
MS.gene027075.t1	AT4G02730	26.852	216	145	9	185	395	34	241	3.86e-11	64.3
MS.gene027075.t1	AT5G56130	23.786	206	113	5	232	395	16	219	7.04e-11	63.5

Find 126 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGATGAAACCTTGAGGTTT+TGG	0.193519	3.2:+44402725	MS.gene027075:CDS
TGACTATTACCTCAATTTAC+TGG	0.257532	3.2:+44401784	MS.gene027075:CDS
GTTGCCACTGTCCAACCATC+TGG	0.273358	3.2:-44402694	None:intergenic
TTACCTCAATTTACTGGATT+GGG	0.283267	3.2:+44401790	MS.gene027075:CDS
AATGTTCTTGCAATTGGATT+AGG	0.295419	3.2:+44401821	MS.gene027075:CDS
CTCCTTCCAGAGAAGCTTAC+AGG	0.301895	3.2:+44401252	MS.gene027075:CDS
ATGCGGCTCAAATGTGAAAA+TGG	0.324208	3.2:-44402805	None:intergenic
AGCGCCAATGTTCTTGCAAT+TGG	0.327966	3.2:+44401815	MS.gene027075:CDS
GTTCTTGAAAGCTAGAATTC+TGG	0.331154	3.2:-44401309	None:intergenic
GCAGACTTCTGAGAGGATAT+TGG	0.341248	3.2:+44401742	MS.gene027075:intron
ATGATAATCAACTTTACATC+TGG	0.350065	3.2:+44402332	MS.gene027075:CDS
AATGGCTCAACTCTTGCTTT+TGG	0.357983	3.2:-44402787	None:intergenic
GCGATGAAACCATCAAGTTT+TGG	0.364930	3.2:+44402470	MS.gene027075:CDS
GGATTGAAATGGTCAGCTTC+TGG	0.370788	3.2:+44402286	MS.gene027075:CDS
ATTACCTCAATTTACTGGAT+TGG	0.371678	3.2:+44401789	MS.gene027075:CDS
TAGCTTCCTGTAAGCTTCTC+TGG	0.375997	3.2:-44401258	None:intergenic
TCTCATTCTTGTTCCACAAC+AGG	0.376647	3.2:-44402549	None:intergenic
GCTCCTGATGGCCGTCATAT+TGG	0.389395	3.2:+44401938	MS.gene027075:CDS
ATTTCAATCCGCAAACTTCT+CGG	0.394694	3.2:-44402273	None:intergenic
AATAATTCGCCGGGGCTTGA+TGG	0.396021	3.2:-44401405	None:intergenic
CCCTATCACATCTGTAAGTT+GGG	0.396604	3.2:+44401916	MS.gene027075:CDS
TCTGCCATCTTCACCATTGA+AGG	0.397901	3.2:-44402628	None:intergenic
CTCAGTTGACACTGGGTCTC+AGG	0.408962	3.2:+44402519	MS.gene027075:CDS
TAGGAAACACATTGTATTTG+TGG	0.412235	3.2:+44401840	MS.gene027075:CDS
CAAGGGAATTTACTAGCTAC+TGG	0.417964	3.2:+44402436	MS.gene027075:CDS
GCCCTATCACATCTGTAAGT+TGG	0.420766	3.2:+44401915	MS.gene027075:CDS
CTCAGGTATGTTCCCTGTTG+TGG	0.426699	3.2:+44402536	MS.gene027075:CDS
TAATAGTCATCCACAATGTC+TGG	0.428065	3.2:-44401770	None:intergenic
GTGGGTATTCCAAAACTTGA+TGG	0.428833	3.2:-44402479	None:intergenic
TAGGCAGCGAGTAGGGTCTT+TGG	0.428851	3.2:+44402147	MS.gene027075:CDS
CAGAAGCTCTAAACATGAAC+AGG	0.436496	3.2:+44401285	MS.gene027075:CDS
AAATCCATAGCTGATCTATT+CGG	0.447023	3.2:-44401161	None:intergenic
GAGAAGTTTGCGGATTGAAA+TGG	0.447588	3.2:+44402275	MS.gene027075:CDS
CTTGCTTGGTGTCCTTTCCA+AGG	0.448593	3.2:+44402418	MS.gene027075:CDS
GCCCAACTTACAGATGTGAT+AGG	0.450468	3.2:-44401917	None:intergenic
GTCAGCTTCTGGTCTACAAT+TGG	0.451980	3.2:+44402297	MS.gene027075:CDS
AGGAAACACATTGTATTTGT+GGG	0.452538	3.2:+44401841	MS.gene027075:CDS
CTTCAACAAGGTGGCTTCAC+AGG	0.460645	3.2:+44402365	MS.gene027075:CDS
CTGCAGTAAAAGCGCTTGCT+TGG	0.476217	3.2:+44402404	MS.gene027075:CDS
CACAGAATCAGATAACACTT+TGG	0.480456	3.2:+44402599	MS.gene027075:CDS
TCATGAGAGAAGAAATCAAC+TGG	0.484334	3.2:-44401347	None:intergenic
ACAACTCTGAAGTGCAGCTA+TGG	0.484474	3.2:+44401972	MS.gene027075:CDS
GGATACAGCTTCAGATAGAC+AGG	0.484963	3.2:+44401994	MS.gene027075:CDS
ATGCCAATATGACGGCCATC+AGG	0.485428	3.2:-44401941	None:intergenic
CTAGCTACTGGCGGAGGCAG+TGG	0.487153	3.2:+44402448	MS.gene027075:CDS
CACTTCAAGAGTTCTTCATA+TGG	0.487383	3.2:+44402663	MS.gene027075:CDS
TTGATTATCATTCCCTCCAC+TGG	0.491219	3.2:-44402320	None:intergenic
TTGCCACTGTCCAACCATCT+GGG	0.492660	3.2:-44402693	None:intergenic
TGGAAGGAGAGCACACTTGT+GGG	0.493184	3.2:-44401238	None:intergenic
ACTTTGGAAATATCCTTCAA+TGG	0.495344	3.2:+44402615	MS.gene027075:CDS
GATGGCCGTCATATTGGCAT+TGG	0.495923	3.2:+44401944	MS.gene027075:CDS
ATATCCTTCAATGGTGAAGA+TGG	0.498891	3.2:+44402624	MS.gene027075:CDS
AACAACCACATACTCACAAC+CGG	0.499451	3.2:+44402175	MS.gene027075:CDS
TTGCTTGGTGTCCTTTCCAA+GGG	0.501380	3.2:+44402419	MS.gene027075:CDS
CGCACTACATGGTGACTGAA+GGG	0.507766	3.2:+44401192	MS.gene027075:CDS
CTGGAAGGAGAGCACACTTG+TGG	0.508404	3.2:-44401239	None:intergenic
AAGAAATCAACTGGCGGCGT+AGG	0.510142	3.2:-44401338	None:intergenic
GGGCATGACCGAGAAGTTTG+CGG	0.518071	3.2:+44402265	MS.gene027075:CDS
CTGCAGTTGAGAACTTTGAA+AGG	0.529944	3.2:+44402118	MS.gene027075:intron
GAACTTTGAAAGGTGGGCAT+AGG	0.530523	3.2:+44402128	MS.gene027075:CDS
GCCCCGGCGAATTATTCCTC+AGG	0.533857	3.2:+44401412	MS.gene027075:CDS
CTCATTCTTGTTCCACAACA+GGG	0.537252	3.2:-44402548	None:intergenic
ACACCTGAGGAATAATTCGC+CGG	0.542147	3.2:-44401415	None:intergenic
TGATAATCAACTTTACATCT+GGG	0.543770	3.2:+44402333	MS.gene027075:CDS
TTTCGATTATGCGCACTACA+TGG	0.544215	3.2:+44401181	MS.gene027075:CDS
TGCTTGAACTCAGTTGACAC+TGG	0.544299	3.2:+44402511	MS.gene027075:CDS
GACAGTGGCAACAGCTGCAG+CGG	0.545471	3.2:+44402705	MS.gene027075:CDS
AGGGAGCTACTCAGCTCTCA+TGG	0.545560	3.2:+44402571	MS.gene027075:CDS
TATTTGTGGGATGCATCAAA+CGG	0.551368	3.2:+44401854	MS.gene027075:CDS
AAATCAACTGGCGGCGTAGG+TGG	0.551699	3.2:-44401335	None:intergenic
CAACTCTGAAGTGCAGCTAT+GGG	0.552386	3.2:+44401973	MS.gene027075:CDS
TAATCCAATTGCAAGAACAT+TGG	0.554610	3.2:-44401819	None:intergenic
CTCACAACCGGAGGGATGGA+TGG	0.555824	3.2:+44402187	MS.gene027075:CDS
CATATGGCGCAGAGCCCAGA+TGG	0.555918	3.2:+44402679	MS.gene027075:CDS
GTGGGCATAGGCAGCGAGTA+GGG	0.557831	3.2:+44402140	MS.gene027075:CDS
CACCTGAGGAATAATTCGCC+GGG	0.559324	3.2:-44401414	None:intergenic
AGTTGAGAACTTTGAAAGGT+GGG	0.559397	3.2:+44402122	MS.gene027075:intron
TGGCGCAGAGCCCAGATGGT+TGG	0.562028	3.2:+44402683	MS.gene027075:CDS
TACCTCAATTTACTGGATTG+GGG	0.562441	3.2:+44401791	MS.gene027075:CDS
GCTAGTAAATTCCCTTGGAA+AGG	0.568517	3.2:-44402430	None:intergenic
GTCTACAATTGGCCAGTGGA+GGG	0.570881	3.2:+44402308	MS.gene027075:CDS
GTGAAGATGGCAGAACTCAA+TGG	0.571074	3.2:+44402637	MS.gene027075:CDS
GCGCACTACATGGTGACTGA+AGG	0.571364	3.2:+44401191	MS.gene027075:CDS
CATACTCACAACCGGAGGGA+TGG	0.571933	3.2:+44402183	MS.gene027075:CDS
CAGTTGAGAACTTTGAAAGG+TGG	0.572101	3.2:+44402121	MS.gene027075:intron
GGTCTACAATTGGCCAGTGG+AGG	0.575013	3.2:+44402307	MS.gene027075:CDS
TTCCCCAATCCAGTAAATTG+AGG	0.576674	3.2:-44401793	None:intergenic
TCCCTCCGGTTGTGAGTATG+TGG	0.579028	3.2:-44402180	None:intergenic
TTCCTGTAAGCTTCTCTGGA+AGG	0.579286	3.2:-44401254	None:intergenic
ACTGAAGGGGCTAAAGCTAA+AGG	0.581037	3.2:+44401206	MS.gene027075:CDS
AGGGGCTAAAGCTAAAGGTA+AGG	0.581254	3.2:+44401211	MS.gene027075:CDS
TCTGTAAGTTGGGCTCCTGA+TGG	0.584539	3.2:+44401926	MS.gene027075:CDS
CCCAACTTACAGATGTGATA+GGG	0.585245	3.2:-44401916	None:intergenic
AATTCTACCATCCATCCCTC+CGG	0.585408	3.2:-44402194	None:intergenic
AAAAGCATTCCAAAACCTCA+AGG	0.587395	3.2:-44402734	None:intergenic
TGCATCAAACGGTTCCACTT+CGG	0.590792	3.2:+44401865	MS.gene027075:CDS
GGTGGGCATAGGCAGCGAGT+AGG	0.596714	3.2:+44402139	MS.gene027075:CDS
CATACCGAATAGATCAGCTA+TGG	0.601151	3.2:+44401157	MS.gene027075:CDS
AGCGAGTAGGGTCTTTGGCA+TGG	0.601597	3.2:+44402152	MS.gene027075:CDS
AACGGTAACAAGTTCCGAAG+TGG	0.605642	3.2:-44401879	None:intergenic
GTTACCGTTGATGATGAAGA+TGG	0.607070	3.2:+44401893	MS.gene027075:CDS
ATTGGATGCTCCAGACATTG+TGG	0.612151	3.2:+44401760	MS.gene027075:CDS
AAGATAAAACCATCAAGCCC+CGG	0.617625	3.2:+44401396	MS.gene027075:CDS
TGTTGTGGAACAAGAATGAG+AGG	0.618210	3.2:+44402551	MS.gene027075:CDS
AGGGCCATCTTCATCATCAA+CGG	0.626287	3.2:-44401897	None:intergenic
GCTTGAACTCAGTTGACACT+GGG	0.627877	3.2:+44402512	MS.gene027075:CDS
CAGTAGCTAGTAAATTCCCT+TGG	0.629888	3.2:-44402435	None:intergenic
AATTTACTAGCTACTGGCGG+AGG	0.633586	3.2:+44402442	MS.gene027075:CDS
TCAAGCCAATGCCAATATGA+CGG	0.636859	3.2:-44401949	None:intergenic
GAGCCCAGATGGTTGGACAG+TGG	0.641101	3.2:+44402690	MS.gene027075:CDS
ATGTTACTGCTTATCGAATG+CGG	0.643437	3.2:-44402822	None:intergenic
ACCACATACTCACAACCGGA+GGG	0.650856	3.2:+44402179	MS.gene027075:CDS
GCACTACATGGTGACTGAAG+GGG	0.656801	3.2:+44401193	MS.gene027075:CDS
GTTCAAGCATGCACCTGTGT+GGG	0.668846	3.2:-44402497	None:intergenic
CTGCAGCGGATGAAACCTTG+AGG	0.678181	3.2:+44402719	MS.gene027075:CDS
AGTTCAAGCATGCACCTGTG+TGG	0.678713	3.2:-44402498	None:intergenic
TCTGGTCTACAATTGGCCAG+TGG	0.684216	3.2:+44402304	MS.gene027075:CDS
ACATTGTTGAAACATACAGA+GGG	0.688874	3.2:+44402245	MS.gene027075:CDS
AACCACATACTCACAACCGG+AGG	0.695066	3.2:+44402178	MS.gene027075:CDS
TGAGAGAAGAAATCAACTGG+CGG	0.700989	3.2:-44401344	None:intergenic
CACATTGTTGAAACATACAG+AGG	0.702329	3.2:+44402244	MS.gene027075:CDS
GGGATAGAAGTACTTCAACA+AGG	0.702662	3.2:+44402353	MS.gene027075:CDS
GGGAATTTACTAGCTACTGG+CGG	0.715639	3.2:+44402439	MS.gene027075:CDS
GTTGTGGAACAAGAATGAGA+GGG	0.727155	3.2:+44402552	MS.gene027075:CDS
ACCTGAGGAATAATTCGCCG+GGG	0.733838	3.2:-44401413	None:intergenic
ATAGAAGTACTTCAACAAGG+TGG	0.753913	3.2:+44402356	MS.gene027075:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTCTCAATTCTTTTTAAAT+GGG	+	chr3.2:44401548-44401567	MS.gene027075:intron	15.0%
!!!	TTTTCTCAATTCTTTTTAAA+TGG	+	chr3.2:44401547-44401566	MS.gene027075:intron	15.0%
!!	AATATTAGAAAACTAGTAGT+AGG	-	chr3.2:44402083-44402102	None:intergenic	20.0%
!!!	ATAATAATGTATCGATTGAA+GGG	-	chr3.2:44402040-44402059	None:intergenic	20.0%
!!!	ATTAGTTTTTGAAAATCAAG+TGG	+	chr3.2:44401514-44401533	MS.gene027075:intron	20.0%
!!!	TTAGTTTTTGAAAATCAAGT+GGG	+	chr3.2:44401515-44401534	MS.gene027075:intron	20.0%
!	ATGATAATCAACTTTACATC+TGG	+	chr3.2:44402332-44402351	MS.gene027075:CDS	25.0%
!	TGATAATCAACTTTACATCT+GGG	+	chr3.2:44402333-44402352	MS.gene027075:CDS	25.0%
!!	CACAAAATTGCAAAATCTTT+TGG	-	chr3.2:44401699-44401718	None:intergenic	25.0%
!!	GATTTGAGAATTTTGAACTT+CGG	+	chr3.2:44401634-44401653	MS.gene027075:intron	25.0%
!!!	CATAATAATGTATCGATTGA+AGG	-	chr3.2:44402041-44402060	None:intergenic	25.0%
!!!	TCTTGTTCTAGTTAATTTGT+TGG	+	chr3.2:44401605-44401624	MS.gene027075:intron	25.0%
	AAATCCATAGCTGATCTATT+CGG	-	chr3.2:44401164-44401183	None:intergenic	30.0%
	AATGTTCTTGCAATTGGATT+AGG	+	chr3.2:44401821-44401840	MS.gene027075:CDS	30.0%
	ACATTGTTGAAACATACAGA+GGG	+	chr3.2:44402245-44402264	MS.gene027075:CDS	30.0%
	AGAAAACTAGTAGTAGGAAA+TGG	-	chr3.2:44402077-44402096	None:intergenic	30.0%
	AGGAAACACATTGTATTTGT+GGG	+	chr3.2:44401841-44401860	MS.gene027075:CDS	30.0%
	ATTACCTCAATTTACTGGAT+TGG	+	chr3.2:44401789-44401808	MS.gene027075:CDS	30.0%
	TAATCCAATTGCAAGAACAT+TGG	-	chr3.2:44401822-44401841	None:intergenic	30.0%
	TAGGAAACACATTGTATTTG+TGG	+	chr3.2:44401840-44401859	MS.gene027075:CDS	30.0%
	TGACTATTACCTCAATTTAC+TGG	+	chr3.2:44401784-44401803	MS.gene027075:CDS	30.0%
	TTACCTCAATTTACTGGATT+GGG	+	chr3.2:44401790-44401809	MS.gene027075:CDS	30.0%
!	ACTTTGGAAATATCCTTCAA+TGG	+	chr3.2:44402615-44402634	MS.gene027075:CDS	30.0%
!!	AGACAGGTTTTATACTTATC+TGG	+	chr3.2:44402010-44402029	MS.gene027075:intron	30.0%
!!	TCTTTTTAAATGGGCTTGTT+TGG	+	chr3.2:44401557-44401576	MS.gene027075:intron	30.0%
	ATATCCTTCAATGGTGAAGA+TGG	+	chr3.2:44402624-44402643	MS.gene027075:CDS	35.0%
	ATTTCAATCCGCAAACTTCT+CGG	-	chr3.2:44402276-44402295	None:intergenic	35.0%
	CACAGAATCAGATAACACTT+TGG	+	chr3.2:44402599-44402618	MS.gene027075:CDS	35.0%
	CACATTGTTGAAACATACAG+AGG	+	chr3.2:44402244-44402263	MS.gene027075:CDS	35.0%
	CACTTCAAGAGTTCTTCATA+TGG	+	chr3.2:44402663-44402682	MS.gene027075:CDS	35.0%
	GTTCTTGAAAGCTAGAATTC+TGG	-	chr3.2:44401312-44401331	None:intergenic	35.0%
	TAATAGTCATCCACAATGTC+TGG	-	chr3.2:44401773-44401792	None:intergenic	35.0%
	TACCTCAATTTACTGGATTG+GGG	+	chr3.2:44401791-44401810	MS.gene027075:CDS	35.0%
	TATTTGTGGGATGCATCAAA+CGG	+	chr3.2:44401854-44401873	MS.gene027075:CDS	35.0%
	TCATGAGAGAAGAAATCAAC+TGG	-	chr3.2:44401350-44401369	None:intergenic	35.0%
!	AGTTGAGAACTTTGAAAGGT+GGG	+	chr3.2:44402122-44402141	MS.gene027075:intron	35.0%
!!	AAAAGCATTCCAAAACCTCA+AGG	-	chr3.2:44402737-44402756	None:intergenic	35.0%
!!	ATAGAAGTACTTCAACAAGG+TGG	+	chr3.2:44402356-44402375	MS.gene027075:CDS	35.0%
!!!	TTGAGGTTTTGGAATGCTTT+TGG	+	chr3.2:44402736-44402755	MS.gene027075:CDS	35.0%
	AACAACCACATACTCACAAC+CGG	+	chr3.2:44402175-44402194	MS.gene027075:CDS	40.0%
	AAGATAAAACCATCAAGCCC+CGG	+	chr3.2:44401396-44401415	MS.gene027075:CDS	40.0%
	ATGCGGCTCAAATGTGAAAA+TGG	-	chr3.2:44402808-44402827	None:intergenic	40.0%
	CAAGGGAATTTACTAGCTAC+TGG	+	chr3.2:44402436-44402455	MS.gene027075:CDS	40.0%
	CAGTAGCTAGTAAATTCCCT+TGG	-	chr3.2:44402438-44402457	None:intergenic	40.0%
	CATACCGAATAGATCAGCTA+TGG	+	chr3.2:44401157-44401176	MS.gene027075:CDS	40.0%
	CCCAACTTACAGATGTGATA+GGG	-	chr3.2:44401919-44401938	None:intergenic	40.0%
	CCCTATCACATCTGTAAGTT+GGG	+	chr3.2:44401916-44401935	MS.gene027075:CDS	40.0%
	CTCATTCTTGTTCCACAACA+GGG	-	chr3.2:44402551-44402570	None:intergenic	40.0%
	CTGCAGTTGAGAACTTTGAA+AGG	+	chr3.2:44402118-44402137	MS.gene027075:intron	40.0%
	GCTAGTAAATTCCCTTGGAA+AGG	-	chr3.2:44402433-44402452	None:intergenic	40.0%
	GTGGGTATTCCAAAACTTGA+TGG	-	chr3.2:44402482-44402501	None:intergenic	40.0%
	GTTGTGGAACAAGAATGAGA+GGG	+	chr3.2:44402552-44402571	MS.gene027075:CDS	40.0%
	TCAAGCCAATGCCAATATGA+CGG	-	chr3.2:44401952-44401971	None:intergenic	40.0%
	TCTCATTCTTGTTCCACAAC+AGG	-	chr3.2:44402552-44402571	None:intergenic	40.0%
	TGAGAGAAGAAATCAACTGG+CGG	-	chr3.2:44401347-44401366	None:intergenic	40.0%
	TGTTGTGGAACAAGAATGAG+AGG	+	chr3.2:44402551-44402570	MS.gene027075:CDS	40.0%
	TTCCCCAATCCAGTAAATTG+AGG	-	chr3.2:44401796-44401815	None:intergenic	40.0%
	TTGATTATCATTCCCTCCAC+TGG	-	chr3.2:44402323-44402342	None:intergenic	40.0%
	TTTCGATTATGCGCACTACA+TGG	+	chr3.2:44401181-44401200	MS.gene027075:CDS	40.0%
!	AATGGCTCAACTCTTGCTTT+TGG	-	chr3.2:44402790-44402809	None:intergenic	40.0%
!	CAGTTGAGAACTTTGAAAGG+TGG	+	chr3.2:44402121-44402140	MS.gene027075:intron	40.0%
!	GAGAAGTTTGCGGATTGAAA+TGG	+	chr3.2:44402275-44402294	MS.gene027075:CDS	40.0%
!	GCGATGAAACCATCAAGTTT+TGG	+	chr3.2:44402470-44402489	MS.gene027075:CDS	40.0%
!!	CAGAAGCTCTAAACATGAAC+AGG	+	chr3.2:44401285-44401304	MS.gene027075:CDS	40.0%
!!	GCAGCTTTTGTTGTAACTTC+TGG	-	chr3.2:44402766-44402785	None:intergenic	40.0%
!!	GGGATAGAAGTACTTCAACA+AGG	+	chr3.2:44402353-44402372	MS.gene027075:CDS	40.0%
!!	GTTACCGTTGATGATGAAGA+TGG	+	chr3.2:44401893-44401912	MS.gene027075:CDS	40.0%
!!	TTGTTTTGCAGACTTCTGAG+AGG	+	chr3.2:44401735-44401754	MS.gene027075:intron	40.0%
!!!	AAGTTTTGGAATACCCACAC+AGG	+	chr3.2:44402484-44402503	MS.gene027075:CDS	40.0%
	AACGGTAACAAGTTCCGAAG+TGG	-	chr3.2:44401882-44401901	None:intergenic	45.0%
	AATTCTACCATCCATCCCTC+CGG	-	chr3.2:44402197-44402216	None:intergenic	45.0%
	AATTTACTAGCTACTGGCGG+AGG	+	chr3.2:44402442-44402461	MS.gene027075:CDS	45.0%
	ACACCTGAGGAATAATTCGC+CGG	-	chr3.2:44401418-44401437	None:intergenic	45.0%
	ACTGAAGGGGCTAAAGCTAA+AGG	+	chr3.2:44401206-44401225	MS.gene027075:CDS	45.0%
	AGCGCCAATGTTCTTGCAAT+TGG	+	chr3.2:44401815-44401834	MS.gene027075:CDS	45.0%
	AGGGCCATCTTCATCATCAA+CGG	-	chr3.2:44401900-44401919	None:intergenic	45.0%
	AGGGGCTAAAGCTAAAGGTA+AGG	+	chr3.2:44401211-44401230	MS.gene027075:CDS	45.0%
	ATTGGATGCTCCAGACATTG+TGG	+	chr3.2:44401760-44401779	MS.gene027075:CDS	45.0%
	GCCCAACTTACAGATGTGAT+AGG	-	chr3.2:44401920-44401939	None:intergenic	45.0%
	GCCCTATCACATCTGTAAGT+TGG	+	chr3.2:44401915-44401934	MS.gene027075:CDS	45.0%
	GCTTGAACTCAGTTGACACT+GGG	+	chr3.2:44402512-44402531	MS.gene027075:CDS	45.0%
	GGATACAGCTTCAGATAGAC+AGG	+	chr3.2:44401994-44402013	MS.gene027075:CDS	45.0%
	GGATTGAAATGGTCAGCTTC+TGG	+	chr3.2:44402286-44402305	MS.gene027075:CDS	45.0%
	GGGAATTTACTAGCTACTGG+CGG	+	chr3.2:44402439-44402458	MS.gene027075:CDS	45.0%
	GTCAGCTTCTGGTCTACAAT+TGG	+	chr3.2:44402297-44402316	MS.gene027075:CDS	45.0%
	GTGAAGATGGCAGAACTCAA+TGG	+	chr3.2:44402637-44402656	MS.gene027075:CDS	45.0%
	TAGCTTCCTGTAAGCTTCTC+TGG	-	chr3.2:44401261-44401280	None:intergenic	45.0%
	TCTGCCATCTTCACCATTGA+AGG	-	chr3.2:44402631-44402650	None:intergenic	45.0%
	TGCATCAAACGGTTCCACTT+CGG	+	chr3.2:44401865-44401884	MS.gene027075:CDS	45.0%
	TGCTTGAACTCAGTTGACAC+TGG	+	chr3.2:44402511-44402530	MS.gene027075:CDS	45.0%
	TTCCTGTAAGCTTCTCTGGA+AGG	-	chr3.2:44401257-44401276	None:intergenic	45.0%
!	CGGATGAAACCTTGAGGTTT+TGG	+	chr3.2:44402725-44402744	MS.gene027075:CDS	45.0%
!	GAACTTTGAAAGGTGGGCAT+AGG	+	chr3.2:44402128-44402147	MS.gene027075:CDS	45.0%
!	GCAGACTTCTGAGAGGATAT+TGG	+	chr3.2:44401742-44401761	MS.gene027075:intron	45.0%
!!	ACAACTCTGAAGTGCAGCTA+TGG	+	chr3.2:44401972-44401991	MS.gene027075:CDS	45.0%
!!	CAACTCTGAAGTGCAGCTAT+GGG	+	chr3.2:44401973-44401992	MS.gene027075:CDS	45.0%
!!	TTGCTTGGTGTCCTTTCCAA+GGG	+	chr3.2:44402419-44402438	MS.gene027075:CDS	45.0%
	AACCACATACTCACAACCGG+AGG	+	chr3.2:44402178-44402197	MS.gene027075:CDS	50.0%
	AAGAAATCAACTGGCGGCGT+AGG	-	chr3.2:44401341-44401360	None:intergenic	50.0%
	AATAATTCGCCGGGGCTTGA+TGG	-	chr3.2:44401408-44401427	None:intergenic	50.0%
	ACCACATACTCACAACCGGA+GGG	+	chr3.2:44402179-44402198	MS.gene027075:CDS	50.0%
	ACCTGAGGAATAATTCGCCG+GGG	-	chr3.2:44401416-44401435	None:intergenic	50.0%
	AGTTCAAGCATGCACCTGTG+TGG	-	chr3.2:44402501-44402520	None:intergenic	50.0%
	ATGCCAATATGACGGCCATC+AGG	-	chr3.2:44401944-44401963	None:intergenic	50.0%
	CACCTGAGGAATAATTCGCC+GGG	-	chr3.2:44401417-44401436	None:intergenic	50.0%
	CGCACTACATGGTGACTGAA+GGG	+	chr3.2:44401192-44401211	MS.gene027075:CDS	50.0%
	CTCAGGTATGTTCCCTGTTG+TGG	+	chr3.2:44402536-44402555	MS.gene027075:CDS	50.0%
	CTCCTTCCAGAGAAGCTTAC+AGG	+	chr3.2:44401252-44401271	MS.gene027075:CDS	50.0%
	CTTCAACAAGGTGGCTTCAC+AGG	+	chr3.2:44402365-44402384	MS.gene027075:CDS	50.0%
	GATGGCCGTCATATTGGCAT+TGG	+	chr3.2:44401944-44401963	MS.gene027075:CDS	50.0%
	GCACTACATGGTGACTGAAG+GGG	+	chr3.2:44401193-44401212	MS.gene027075:CDS	50.0%
	GTCTACAATTGGCCAGTGGA+GGG	+	chr3.2:44402308-44402327	MS.gene027075:CDS	50.0%
	GTTCAAGCATGCACCTGTGT+GGG	-	chr3.2:44402500-44402519	None:intergenic	50.0%
	TCTGGTCTACAATTGGCCAG+TGG	+	chr3.2:44402304-44402323	MS.gene027075:CDS	50.0%
	TTGCCACTGTCCAACCATCT+GGG	-	chr3.2:44402696-44402715	None:intergenic	50.0%
!	TCTGTAAGTTGGGCTCCTGA+TGG	+	chr3.2:44401926-44401945	MS.gene027075:CDS	50.0%
!	TGGAAGGAGAGCACACTTGT+GGG	-	chr3.2:44401241-44401260	None:intergenic	50.0%
!!	CTGCAGTAAAAGCGCTTGCT+TGG	+	chr3.2:44402404-44402423	MS.gene027075:CDS	50.0%
!!	CTTGCTTGGTGTCCTTTCCA+AGG	+	chr3.2:44402418-44402437	MS.gene027075:CDS	50.0%
	AAATCAACTGGCGGCGTAGG+TGG	-	chr3.2:44401338-44401357	None:intergenic	55.0%
	AGCGAGTAGGGTCTTTGGCA+TGG	+	chr3.2:44402152-44402171	MS.gene027075:CDS	55.0%
	AGGGAGCTACTCAGCTCTCA+TGG	+	chr3.2:44402571-44402590	MS.gene027075:CDS	55.0%
	CACACACACACACACACCTG+AGG	-	chr3.2:44401431-44401450	None:intergenic	55.0%
	CATACTCACAACCGGAGGGA+TGG	+	chr3.2:44402183-44402202	MS.gene027075:CDS	55.0%
	CTCAGTTGACACTGGGTCTC+AGG	+	chr3.2:44402519-44402538	MS.gene027075:CDS	55.0%
	CTGCAGCGGATGAAACCTTG+AGG	+	chr3.2:44402719-44402738	MS.gene027075:CDS	55.0%
	GCGCACTACATGGTGACTGA+AGG	+	chr3.2:44401191-44401210	MS.gene027075:CDS	55.0%
	GCTCCTGATGGCCGTCATAT+TGG	+	chr3.2:44401938-44401957	MS.gene027075:CDS	55.0%
	GGGCATGACCGAGAAGTTTG+CGG	+	chr3.2:44402265-44402284	MS.gene027075:CDS	55.0%
	GGTCTACAATTGGCCAGTGG+AGG	+	chr3.2:44402307-44402326	MS.gene027075:CDS	55.0%
	GTTGCCACTGTCCAACCATC+TGG	-	chr3.2:44402697-44402716	None:intergenic	55.0%
	TAGGCAGCGAGTAGGGTCTT+TGG	+	chr3.2:44402147-44402166	MS.gene027075:CDS	55.0%
	TCCCTCCGGTTGTGAGTATG+TGG	-	chr3.2:44402183-44402202	None:intergenic	55.0%
!	CTGGAAGGAGAGCACACTTG+TGG	-	chr3.2:44401242-44401261	None:intergenic	55.0%
	CATATGGCGCAGAGCCCAGA+TGG	+	chr3.2:44402679-44402698	MS.gene027075:CDS	60.0%
	CTCACAACCGGAGGGATGGA+TGG	+	chr3.2:44402187-44402206	MS.gene027075:CDS	60.0%
	GACAGTGGCAACAGCTGCAG+CGG	+	chr3.2:44402705-44402724	MS.gene027075:CDS	60.0%
	GAGCCCAGATGGTTGGACAG+TGG	+	chr3.2:44402690-44402709	MS.gene027075:CDS	60.0%
	GCCCCGGCGAATTATTCCTC+AGG	+	chr3.2:44401412-44401431	MS.gene027075:CDS	60.0%
	GTGGGCATAGGCAGCGAGTA+GGG	+	chr3.2:44402140-44402159	MS.gene027075:CDS	60.0%
	CTAGCTACTGGCGGAGGCAG+TGG	+	chr3.2:44402448-44402467	MS.gene027075:CDS	65.0%
	GGTGGGCATAGGCAGCGAGT+AGG	+	chr3.2:44402139-44402158	MS.gene027075:CDS	65.0%
	TGGCGCAGAGCCCAGATGGT+TGG	+	chr3.2:44402683-44402702	MS.gene027075:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	44401146	44402834	44401146	ID=MS.gene027075
chr3.2	mRNA	44401146	44402834	44401146	ID=MS.gene027075.t1;Parent=MS.gene027075
chr3.2	exon	44401146	44401433	44401146	ID=MS.gene027075.t1.exon1;Parent=MS.gene027075.t1
chr3.2	CDS	44401146	44401433	44401146	ID=cds.MS.gene027075.t1;Parent=MS.gene027075.t1
chr3.2	exon	44401746	44402015	44401746	ID=MS.gene027075.t1.exon2;Parent=MS.gene027075.t1
chr3.2	CDS	44401746	44402015	44401746	ID=cds.MS.gene027075.t1;Parent=MS.gene027075.t1
chr3.2	exon	44402124	44402834	44402124	ID=MS.gene027075.t1.exon3;Parent=MS.gene027075.t1
chr3.2	CDS	44402124	44402834	44402124	ID=cds.MS.gene027075.t1;Parent=MS.gene027075.t1

Gene Sequence

>MS.gene027075

TTGGATAGATTCATACCGAATAGATCAGCTATGGATTTCGATTATGCGCACTACATGGTGACTGAAGGGGCTAAAGCTAAAGGTAAGGAAAACCCACAAGTGTGCTCTCCTTCCAGAGAAGCTTACAGGAAGCTACTTGCAGAAGCTCTAAACATGAACAGGACCAGAATTCTAGCTTTCAAGAACAAGCCACCTACGCCGCCAGTTGATTTCTTCTCTCATGATATCATTACTTCATCAACTCTTCGACAAGATAAAACCATCAAGCCCCGGCGAATTATTCCTCAGGTGTGTGTGTGTGTGTGTGTGTTTCTTAATGATATCTGATTCATATTTCATATATTTTATCATTATTAATTTCGTACTTGATTAGTTTTTGAAAATCAAGTGGGTCTGAGTTGTTTTCTCAATTCTTTTTAAATGGGCTTGTTTGGTGTGTGAAATCAAAATATTTTGCAATCTTGTTCTAGTTAATTTGTTGGTTATCTGATTTGAGAATTTTGAACTTCGGTTTTCTTAATTCTTATCTGAAAGTGTTGTGTGTTGTAAACCAAAAGATTTTGCAATTTTGTGCTAATTGATTGATTTGTTGTTTTGCAGACTTCTGAGAGGATATTGGATGCTCCAGACATTGTGGATGACTATTACCTCAATTTACTGGATTGGGGAAGCGCCAATGTTCTTGCAATTGGATTAGGAAACACATTGTATTTGTGGGATGCATCAAACGGTTCCACTTCGGAACTTGTTACCGTTGATGATGAAGATGGCCCTATCACATCTGTAAGTTGGGCTCCTGATGGCCGTCATATTGGCATTGGCTTGAACAACTCTGAAGTGCAGCTATGGGATACAGCTTCAGATAGACAGGTTTTATACTTATCTGGTTTACCCTTCAATCGATACATTATTATGAGCTATTCAGTATCCATTTCCTACTACTAGTTTTCTAATATTATTCATGTTATGTTTCTGCAGTTGAGAACTTTGAAAGGTGGGCATAGGCAGCGAGTAGGGTCTTTGGCATGGAACAACCACATACTCACAACCGGAGGGATGGATGGTAGAATTATCAATAATGATGTAAGAATTAGAGCACACATTGTTGAAACATACAGAGGGCATGACCGAGAAGTTTGCGGATTGAAATGGTCAGCTTCTGGTCTACAATTGGCCAGTGGAGGGAATGATAATCAACTTTACATCTGGGATAGAAGTACTTCAACAAGGTGGCTTCACAGGCTTGAAGATCATACATCTGCAGTAAAAGCGCTTGCTTGGTGTCCTTTCCAAGGGAATTTACTAGCTACTGGCGGAGGCAGTGGCGATGAAACCATCAAGTTTTGGAATACCCACACAGGTGCATGCTTGAACTCAGTTGACACTGGGTCTCAGGTATGTTCCCTGTTGTGGAACAAGAATGAGAGGGAGCTACTCAGCTCTCATGGTTTTACACAGAATCAGATAACACTTTGGAAATATCCTTCAATGGTGAAGATGGCAGAACTCAATGGTCACACTTCAAGAGTTCTTCATATGGCGCAGAGCCCAGATGGTTGGACAGTGGCAACAGCTGCAGCGGATGAAACCTTGAGGTTTTGGAATGCTTTTGGCACTCCAGAAGTTACAACAAAAGCTGCACCAAAAGCAAGAGTTGAGCCATTTTCACATTTGAGCCGCATTCGATAA

Protein sequence

>MS.gene027075.t1

LDRFIPNRSAMDFDYAHYMVTEGAKAKGKENPQVCSPSREAYRKLLAEALNMNRTRILAFKNKPPTPPVDFFSHDIITSSTLRQDKTIKPRRIIPQTSERILDAPDIVDDYYLNLLDWGSANVLAIGLGNTLYLWDASNGSTSELVTVDDEDGPITSVSWAPDGRHIGIGLNNSEVQLWDTASDRQLRTLKGGHRQRVGSLAWNNHILTTGGMDGRIINNDVRIRAHIVETYRGHDREVCGLKWSASGLQLASGGNDNQLYIWDRSTSTRWLHRLEDHTSAVKALAWCPFQGNLLATGGGSGDETIKFWNTHTGACLNSVDTGSQVCSLLWNKNERELLSSHGFTQNQITLWKYPSMVKMAELNGHTSRVLHMAQSPDGWTVATAAADETLRFWNAFGTPEVTTKAAPKARVEPFSHLSRIR