AlfalfaGEDB | MS.gene062885

M. sativa cultivar XinJiangDaYe / MS.gene062885

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062885.t1	XP_003607005.1	75.7	181	35	3	1	177	1	176	8.90E-49	203.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062885.t1	G7JIL9	75.7	181	35	3	1	177	1	176	6.4e-49	203.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene062885	0.804157	2.47E-49	-1.69E-46
MS.gene049159	MS.gene062885	0.851323	9.56E-61	-1.69E-46
MS.gene049254	MS.gene062885	0.805263	1.45E-49	-1.69E-46
MS.gene049419	MS.gene062885	-0.80138	9.25E-49	-1.69E-46
MS.gene049452	MS.gene062885	0.83217	1.10E-55	-1.69E-46
MS.gene049983	MS.gene062885	0.810197	1.30E-50	-1.69E-46
MS.gene050605	MS.gene062885	0.809506	1.83E-50	-1.69E-46
MS.gene050654	MS.gene062885	0.802061	6.71E-49	-1.69E-46
MS.gene050737	MS.gene062885	0.802882	4.54E-49	-1.69E-46
MS.gene050941	MS.gene062885	0.821716	3.48E-53	-1.69E-46
MS.gene050953	MS.gene062885	0.804258	2.36E-49	-1.69E-46
MS.gene051452	MS.gene062885	0.874148	8.47E-68	-1.69E-46
MS.gene051975	MS.gene062885	0.819068	1.41E-52	-1.69E-46
MS.gene052012	MS.gene062885	0.807586	4.70E-50	-1.69E-46
MS.gene052426	MS.gene062885	0.814778	1.30E-51	-1.69E-46
MS.gene052519	MS.gene062885	0.812715	3.69E-51	-1.69E-46
MS.gene052863	MS.gene062885	0.835462	1.65E-56	-1.69E-46
MS.gene052954	MS.gene062885	0.812668	3.78E-51	-1.69E-46
MS.gene053006	MS.gene062885	0.80935	1.98E-50	-1.69E-46
MS.gene053008	MS.gene062885	0.82038	7.07E-53	-1.69E-46
MS.gene05350	MS.gene062885	0.803682	3.10E-49	-1.69E-46
MS.gene053931	MS.gene062885	0.800805	1.21E-48	-1.69E-46
MS.gene05415	MS.gene062885	0.806752	7.06E-50	-1.69E-46
MS.gene054236	MS.gene062885	0.808555	2.92E-50	-1.69E-46
MS.gene054405	MS.gene062885	0.801096	1.06E-48	-1.69E-46
MS.gene054479	MS.gene062885	0.80179	7.62E-49	-1.69E-46
MS.gene054651	MS.gene062885	0.816698	4.84E-52	-1.69E-46
MS.gene054657	MS.gene062885	0.80214	6.46E-49	-1.69E-46
MS.gene05510	MS.gene062885	0.839489	1.54E-57	-1.69E-46
MS.gene055222	MS.gene062885	0.815311	9.87E-52	-1.69E-46
MS.gene055310	MS.gene062885	0.805462	1.32E-49	-1.69E-46
MS.gene055623	MS.gene062885	0.829877	4.03E-55	-1.69E-46
MS.gene055701	MS.gene062885	0.827375	1.62E-54	-1.69E-46
MS.gene055818	MS.gene062885	0.82891	6.91E-55	-1.69E-46
MS.gene056506	MS.gene062885	0.811362	7.27E-51	-1.69E-46
MS.gene056699	MS.gene062885	0.825087	5.68E-54	-1.69E-46
MS.gene056787	MS.gene062885	0.801265	9.77E-49	-1.69E-46
MS.gene056986	MS.gene062885	0.801812	7.55E-49	-1.69E-46
MS.gene057034	MS.gene062885	0.801003	1.11E-48	-1.69E-46
MS.gene057208	MS.gene062885	0.837632	4.64E-57	-1.69E-46
MS.gene057855	MS.gene062885	0.823205	1.57E-53	-1.69E-46
MS.gene058009	MS.gene062885	0.80038	1.48E-48	-1.69E-46
MS.gene058010	MS.gene062885	0.828003	1.14E-54	-1.69E-46
MS.gene058081	MS.gene062885	0.806395	8.40E-50	-1.69E-46
MS.gene058146	MS.gene062885	0.83617	1.09E-56	-1.69E-46
MS.gene058548	MS.gene062885	0.804334	2.27E-49	-1.69E-46
MS.gene058595	MS.gene062885	0.834205	3.43E-56	-1.69E-46
MS.gene058783	MS.gene062885	0.825043	5.81E-54	-1.69E-46
MS.gene058979	MS.gene062885	0.827998	1.15E-54	-1.69E-46
MS.gene059283	MS.gene062885	0.821614	3.68E-53	-1.69E-46
MS.gene059842	MS.gene062885	0.83536	1.76E-56	-1.69E-46
MS.gene060011	MS.gene062885	0.832577	8.73E-56	-1.69E-46
MS.gene060296	MS.gene062885	0.803792	2.94E-49	-1.69E-46
MS.gene060310	MS.gene062885	0.835503	1.62E-56	-1.69E-46
MS.gene060322	MS.gene062885	0.809336	1.99E-50	-1.69E-46
MS.gene060527	MS.gene062885	0.808099	3.66E-50	-1.69E-46
MS.gene060685	MS.gene062885	0.800769	1.23E-48	-1.69E-46
MS.gene060777	MS.gene062885	0.816026	6.84E-52	-1.69E-46
MS.gene060802	MS.gene062885	0.807629	4.60E-50	-1.69E-46
MS.gene060803	MS.gene062885	0.813847	2.08E-51	-1.69E-46
MS.gene060935	MS.gene062885	0.822851	1.90E-53	-1.69E-46
MS.gene060937	MS.gene062885	0.810894	9.19E-51	-1.69E-46
MS.gene060998	MS.gene062885	0.831716	1.43E-55	-1.69E-46
MS.gene06121	MS.gene062885	0.803696	3.08E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062885.t1	MTR_4g071120	83.978	181	20	4	1	177	1	176	2.27e-87	254

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062885.t1	AT4G34630	54.217	83	34	2	95	175	117	197	2.70e-19	81.3

Find 35 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGATGATTCTTTCATTTCT+TGG	0.184142	4.1:-31850858	MS.gene062885:CDS
GAGACAGAGGATGAGGAAAT+TGG	0.199364	4.1:-31850629	MS.gene062885:CDS
CGATGGGTTATTCGTTCAAT+TGG	0.268599	4.1:+31850811	None:intergenic
AGACAGAGGATGAGGAAATT+GGG	0.300950	4.1:-31850628	MS.gene062885:CDS
TGGTCAAATTCTTCTATAAT+CGG	0.322860	4.1:-31850838	MS.gene062885:CDS
TGGGATATAGGGAACAAGTT+AGG	0.331295	4.1:-31850652	MS.gene062885:CDS
GAGCTTGTTCCATGGGATAT+AGG	0.337033	4.1:-31850664	MS.gene062885:CDS
AGCTTGTTCCATGGGATATA+GGG	0.363293	4.1:-31850663	MS.gene062885:CDS
ATGCTGATTCTCTTCTTCAA+TGG	0.381531	4.1:-31850954	MS.gene062885:CDS
TAATGAATTGAACTTGTTCA+AGG	0.383807	4.1:+31850986	None:intergenic
TGCTGATTCTCTTCTTCAAT+GGG	0.397374	4.1:-31850953	MS.gene062885:CDS
AGATGATGGTGATGATGATT+TGG	0.404444	4.1:-31850692	MS.gene062885:CDS
TCGATGATGATGATGAAGTT+TGG	0.426925	4.1:+31851022	None:intergenic
GGGATATAGGGAACAAGTTA+GGG	0.447825	4.1:-31850651	MS.gene062885:CDS
GGATGAGGAAATTGGGGAAA+AGG	0.455028	4.1:-31850621	MS.gene062885:CDS
CTTCACTTTCATCATCATCA+TGG	0.456100	4.1:+31850725	None:intergenic
GATGAGGAAATTGGGGAAAA+GGG	0.462244	4.1:-31850620	MS.gene062885:CDS
GATGGGTTATTCGTTCAATT+GGG	0.466090	4.1:+31850812	None:intergenic
GTGAGGCCTGGTTGTGTTCG+TGG	0.487697	4.1:-31850553	MS.gene062885:CDS
TGTTCGTGGCAAACATGGCA+TGG	0.512051	4.1:-31850539	MS.gene062885:CDS
GGATGATGAGCTTGTTCCAT+GGG	0.527521	4.1:-31850671	MS.gene062885:CDS
GACAGAGGATGAGGAAATTG+GGG	0.528458	4.1:-31850627	MS.gene062885:CDS
AGTTAGGGAGACAGAGGATG+AGG	0.528796	4.1:-31850636	MS.gene062885:CDS
CTGGGTCATCGAGATGAATA+CGG	0.538229	4.1:+31850788	None:intergenic
AACTTGTTCCCTATATCCCA+TGG	0.564453	4.1:+31850655	None:intergenic
CATCGAGATGAATACGGCGA+TGG	0.565334	4.1:+31850794	None:intergenic
TGGATGATGAGCTTGTTCCA+TGG	0.581692	4.1:-31850672	MS.gene062885:CDS
GTTCTTCACTGTAGCCAACA+TGG	0.585726	4.1:+31850755	None:intergenic
ATCGAGATGAATACGGCGAT+GGG	0.587715	4.1:+31850795	None:intergenic
GAAGAAGATGATGAAGATGA+TGG	0.597886	4.1:-31850706	MS.gene062885:CDS
TGTTTGCCACGAACACAACC+AGG	0.597931	4.1:+31850547	None:intergenic
GGTTGTGTTCGTGGCAAACA+TGG	0.602974	4.1:-31850544	MS.gene062885:CDS
GGAACAAGTTAGGGAGACAG+AGG	0.614922	4.1:-31850642	MS.gene062885:CDS
CCAGTTGAAAATCACCATGT+TGG	0.626221	4.1:-31850769	MS.gene062885:CDS
GTTCGTGGCAAACATGGCAT+GGG	0.631538	4.1:-31850538	MS.gene062885:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATGATGATTCTTTCATTTCT+TGG	-	chr4.1:31850691-31850710	MS.gene062885:CDS	25.0%
!	CAAAAATGAATAATTCGAAG+AGG	-	chr4.1:31850958-31850977	MS.gene062885:CDS	25.0%
!	TGGTCAAATTCTTCTATAAT+CGG	-	chr4.1:31850711-31850730	MS.gene062885:CDS	25.0%
!!	TAATGAATTGAACTTGTTCA+AGG	+	chr4.1:31850566-31850585	None:intergenic	25.0%
	AGAAGAAGAAGAAGAAGAAA+GGG	-	chr4.1:31850653-31850672	MS.gene062885:CDS	30.0%
	CAGAAGAAGAAGAAGAAGAA+AGG	-	chr4.1:31850652-31850671	MS.gene062885:CDS	35.0%
	CTTCACTTTCATCATCATCA+TGG	+	chr4.1:31850827-31850846	None:intergenic	35.0%
	GAAGAAGATGATGAAGATGA+TGG	-	chr4.1:31850843-31850862	MS.gene062885:CDS	35.0%
!	AGATGATGGTGATGATGATT+TGG	-	chr4.1:31850857-31850876	MS.gene062885:CDS	35.0%
!	CAACATGGTGATTTTCAACT+GGG	+	chr4.1:31850782-31850801	None:intergenic	35.0%
!	TCGATGATGATGATGAAGTT+TGG	+	chr4.1:31850530-31850549	None:intergenic	35.0%
!!	ATGCTGATTCTCTTCTTCAA+TGG	-	chr4.1:31850595-31850614	MS.gene062885:CDS	35.0%
!!	GATGGGTTATTCGTTCAATT+GGG	+	chr4.1:31850740-31850759	None:intergenic	35.0%
!!	TGCTGATTCTCTTCTTCAAT+GGG	-	chr4.1:31850596-31850615	MS.gene062885:CDS	35.0%
	AGACAGAGGATGAGGAAATT+GGG	-	chr4.1:31850921-31850940	MS.gene062885:CDS	40.0%
	AGGCCTCACAAAAAGAATAG+GGG	+	chr4.1:31850985-31851004	None:intergenic	40.0%
	CAGGCCTCACAAAAAGAATA+GGG	+	chr4.1:31850986-31851005	None:intergenic	40.0%
	CCAGTTGAAAATCACCATGT+TGG	-	chr4.1:31850780-31850799	MS.gene062885:CDS	40.0%
	GATGAGGAAATTGGGGAAAA+GGG	-	chr4.1:31850929-31850948	MS.gene062885:CDS	40.0%
	GGGATATAGGGAACAAGTTA+GGG	-	chr4.1:31850898-31850917	MS.gene062885:CDS	40.0%
	TGGGATATAGGGAACAAGTT+AGG	-	chr4.1:31850897-31850916	MS.gene062885:CDS	40.0%
!	AACTTGTTCCCTATATCCCA+TGG	+	chr4.1:31850897-31850916	None:intergenic	40.0%
!	AGCTTGTTCCATGGGATATA+GGG	-	chr4.1:31850886-31850905	MS.gene062885:CDS	40.0%
!	CCAACATGGTGATTTTCAAC+TGG	+	chr4.1:31850783-31850802	None:intergenic	40.0%
!!	CGATGGGTTATTCGTTCAAT+TGG	+	chr4.1:31850741-31850760	None:intergenic	40.0%
	ATCGAGATGAATACGGCGAT+GGG	+	chr4.1:31850757-31850776	None:intergenic	45.0%
	CCAGGCCTCACAAAAAGAAT+AGG	+	chr4.1:31850987-31851006	None:intergenic	45.0%
	CTGGGTCATCGAGATGAATA+CGG	+	chr4.1:31850764-31850783	None:intergenic	45.0%
	GACAGAGGATGAGGAAATTG+GGG	-	chr4.1:31850922-31850941	MS.gene062885:CDS	45.0%
	GAGACAGAGGATGAGGAAAT+TGG	-	chr4.1:31850920-31850939	MS.gene062885:CDS	45.0%
	GGATGAGGAAATTGGGGAAA+AGG	-	chr4.1:31850928-31850947	MS.gene062885:CDS	45.0%
	GTTCTTCACTGTAGCCAACA+TGG	+	chr4.1:31850797-31850816	None:intergenic	45.0%
!	CCTATTCTTTTTGTGAGGCC+TGG	-	chr4.1:31850984-31851003	MS.gene062885:CDS	45.0%
!	GAGCTTGTTCCATGGGATAT+AGG	-	chr4.1:31850885-31850904	MS.gene062885:CDS	45.0%
!	GGATGATGAGCTTGTTCCAT+GGG	-	chr4.1:31850878-31850897	MS.gene062885:CDS	45.0%
!	GGTCCCCTATTCTTTTTGTG+AGG	-	chr4.1:31850979-31850998	MS.gene062885:CDS	45.0%
!	TGGATGATGAGCTTGTTCCA+TGG	-	chr4.1:31850877-31850896	MS.gene062885:CDS	45.0%
	AGTTAGGGAGACAGAGGATG+AGG	-	chr4.1:31850913-31850932	MS.gene062885:CDS	50.0%
	CATCGAGATGAATACGGCGA+TGG	+	chr4.1:31850758-31850777	None:intergenic	50.0%
	GGAACAAGTTAGGGAGACAG+AGG	-	chr4.1:31850907-31850926	MS.gene062885:CDS	50.0%
	GGTTGTGTTCGTGGCAAACA+TGG	-	chr4.1:31851005-31851024	MS.gene062885:CDS	50.0%
	GTTCGTGGCAAACATGGCAT+GGG	-	chr4.1:31851011-31851030	MS.gene062885:CDS	50.0%
	TGTTCGTGGCAAACATGGCA+TGG	-	chr4.1:31851010-31851029	MS.gene062885:CDS	50.0%
	TGTTTGCCACGAACACAACC+AGG	+	chr4.1:31851005-31851024	None:intergenic	50.0%
	GTGAGGCCTGGTTGTGTTCG+TGG	-	chr4.1:31850996-31851015	MS.gene062885:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	31850519	31851052	31850519	ID=MS.gene062885
chr4.1	mRNA	31850519	31851052	31850519	ID=MS.gene062885.t1;Parent=MS.gene062885
chr4.1	exon	31850519	31851052	31850519	ID=MS.gene062885.t1.exon1;Parent=MS.gene062885.t1
chr4.1	CDS	31850519	31851052	31850519	ID=cds.MS.gene062885.t1;Parent=MS.gene062885.t1

Gene Sequence

>MS.gene062885

ATGGAAAACCAAACTTCATCATCATCATCGATGCAGTTTCTCATCCTTGAACAAGTTCAATTCATTAAAGTGAATGATGCTGATTCTCTTCTTCAATGGGAACTTGTTGATGTTGTTGACGCTGAAGAACAATCAGAAGAAGAAGAAGAAGAAAGGGTTGATGTTGATGATGATGATGATTCTTTCATTTCTTGGTCAAATTCTTCTATAATCGGTGACCCAATTGAACGAATAACCCATCGCCGTATTCATCTCGATGACCCAGTTGAAAATCACCATGTTGGCTACAGTGAAGAACAAGTTAGCCATGATGATGATGAAAGTGAAGAAGATGATGAAGATGATGGTGATGATGATTTGGATGATGAGCTTGTTCCATGGGATATAGGGAACAAGTTAGGGAGACAGAGGATGAGGAAATTGGGGAAAAGGGTTTCTTCAAAAATGAATAATTCGAAGAGGTCCCCTATTCTTTTTGTGAGGCCTGGTTGTGTTCGTGGCAAACATGGCATGGGTCTTAAACACTGTTTTTAG

Protein sequence

>MS.gene062885.t1

MENQTSSSSSMQFLILEQVQFIKVNDADSLLQWELVDVVDAEEQSEEEEEERVDVDDDDDSFISWSNSSIIGDPIERITHRRIHLDDPVENHHVGYSEEQVSHDDDESEEDDEDDGDDDLDDELVPWDIGNKLGRQRMRKLGKRVSSKMNNSKRSPILFVRPGCVRGKHGMGLKHCF