AlfalfaGEDB | MS.gene068553

M. sativa cultivar XinJiangDaYe / MS.gene068553

KEGG: K02918
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00831.26

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068553.t1	XP_004494985.1	97	99	3	0	1	99	1	99	1.50E-40	175.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068553.t1	Q9M5L0	91.9	99	8	0	1	99	1	99	4.8e-40	164.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068553.t1	A0A1S2XV31	97.0	99	3	0	1	99	1	99	1.1e-40	175.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049178	MS.gene068553	0.806494	8.00E-50	-1.69E-46
MS.gene049341	MS.gene068553	0.816819	4.54E-52	-1.69E-46
MS.gene049756	MS.gene068553	0.810226	1.28E-50	-1.69E-46
MS.gene049825	MS.gene068553	0.827838	1.25E-54	-1.69E-46
MS.gene049873	MS.gene068553	0.800632	1.32E-48	-1.69E-46
MS.gene049887	MS.gene068553	0.804519	2.08E-49	-1.69E-46
MS.gene050277	MS.gene068553	0.855529	5.93E-62	-1.69E-46
MS.gene050303	MS.gene068553	0.810536	1.10E-50	-1.69E-46
MS.gene050719	MS.gene068553	0.818126	2.31E-52	-1.69E-46
MS.gene050721	MS.gene068553	0.818353	2.05E-52	-1.69E-46
MS.gene050724	MS.gene068553	0.81074	9.92E-51	-1.69E-46
MS.gene050780	MS.gene068553	0.803565	3.28E-49	-1.69E-46
MS.gene050807	MS.gene068553	0.808734	2.68E-50	-1.69E-46
MS.gene051063	MS.gene068553	0.843816	1.11E-58	-1.69E-46
MS.gene051247	MS.gene068553	0.801465	8.89E-49	-1.69E-46
MS.gene051330	MS.gene068553	0.814682	1.36E-51	-1.69E-46
MS.gene051633	MS.gene068553	0.821627	3.65E-53	-1.69E-46
MS.gene051700	MS.gene068553	0.824467	7.95E-54	-1.69E-46
MS.gene051746	MS.gene068553	0.855472	6.17E-62	-1.69E-46
MS.gene051748	MS.gene068553	0.847429	1.16E-59	-1.69E-46
MS.gene051757	MS.gene068553	0.824884	6.34E-54	-1.69E-46
MS.gene051903	MS.gene068553	0.84144	4.75E-58	-1.69E-46
MS.gene052133	MS.gene068553	0.843726	1.17E-58	-1.69E-46
MS.gene052202	MS.gene068553	0.800429	1.45E-48	-1.69E-46
MS.gene052286	MS.gene068553	0.807201	5.68E-50	-1.69E-46
MS.gene052302	MS.gene068553	0.832823	7.59E-56	-1.69E-46
MS.gene052376	MS.gene068553	0.832875	7.36E-56	-1.69E-46
MS.gene052665	MS.gene068553	0.800847	1.19E-48	-1.69E-46
MS.gene052856	MS.gene068553	0.816279	6.00E-52	-1.69E-46
MS.gene052858	MS.gene068553	0.800251	1.57E-48	-1.69E-46
MS.gene052879	MS.gene068553	0.814611	1.41E-51	-1.69E-46
MS.gene052947	MS.gene068553	0.838868	2.23E-57	-1.69E-46
MS.gene053109	MS.gene068553	0.826071	3.32E-54	-1.69E-46
MS.gene053437	MS.gene068553	0.82827	9.87E-55	-1.69E-46
MS.gene055468	MS.gene068553	0.801415	9.10E-49	-1.69E-46
MS.gene055512	MS.gene068553	0.800456	1.43E-48	-1.69E-46
MS.gene055580	MS.gene068553	-0.814032	1.89E-51	-1.69E-46
MS.gene055808	MS.gene068553	0.812461	4.19E-51	-1.69E-46
MS.gene056043	MS.gene068553	-0.823054	1.70E-53	-1.69E-46
MS.gene056075	MS.gene068553	0.812128	4.95E-51	-1.69E-46
MS.gene056091	MS.gene068553	0.830857	2.32E-55	-1.69E-46
MS.gene056432	MS.gene068553	0.852875	3.46E-61	-1.69E-46
MS.gene056631	MS.gene068553	0.876643	1.19E-68	-1.69E-46
MS.gene056760	MS.gene068553	0.816113	6.54E-52	-1.69E-46
MS.gene057057	MS.gene068553	0.809997	1.43E-50	-1.69E-46
MS.gene057094	MS.gene068553	0.824629	7.28E-54	-1.69E-46
MS.gene057140	MS.gene068553	0.810734	9.95E-51	-1.69E-46
MS.gene057234	MS.gene068553	0.805702	1.18E-49	-1.69E-46
MS.gene058084	MS.gene068553	0.821838	3.26E-53	-1.69E-46
MS.gene058238	MS.gene068553	0.805999	1.02E-49	-1.69E-46
MS.gene058906	MS.gene068553	0.881569	2.15E-70	-1.69E-46
MS.gene058911	MS.gene068553	0.807768	4.30E-50	-1.69E-46
MS.gene05954	MS.gene068553	0.803954	2.72E-49	-1.69E-46
MS.gene059898	MS.gene068553	0.807141	5.84E-50	-1.69E-46
MS.gene06001	MS.gene068553	0.902003	1.51E-78	-1.69E-46
MS.gene060165	MS.gene068553	0.824816	6.58E-54	-1.69E-46
MS.gene060429	MS.gene068553	0.827582	1.44E-54	-1.69E-46
MS.gene060619	MS.gene068553	0.825091	5.66E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068553.t1	MTR_1g067490	95.833	96	4	0	4	99	3	98	1.89e-59	178
MS.gene068553.t1	MTR_4g062410	88.889	99	11	0	1	99	1	99	1.61e-56	171
MS.gene068553.t1	MTR_1g115545	92.708	96	7	0	4	99	2	97	2.05e-56	170
MS.gene068553.t1	MTR_7g080090	86.869	99	13	0	1	99	1	99	6.84e-54	164
MS.gene068553.t1	MTR_4g072410	85.417	96	14	0	5	100	3	98	2.56e-51	157
MS.gene068553.t1	MTR_1g105880	73.256	86	12	1	14	99	5	79	1.73e-34	114
MS.gene068553.t1	MTR_5g045540	87.879	33	4	0	67	99	2	34	4.02e-13	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068553.t1	AT5G02610	87.879	99	12	0	1	99	1	99	6.85e-55	166
MS.gene068553.t1	AT2G39390	87.000	100	13	0	1	100	1	100	1.61e-54	166
MS.gene068553.t1	AT3G09500	87.000	100	13	0	1	100	1	100	1.68e-54	166
MS.gene068553.t1	AT3G55170	86.000	100	14	0	1	100	1	100	7.03e-54	163
MS.gene068553.t1	AT3G55170	86.000	100	14	0	1	100	1	100	7.03e-54	163
MS.gene068553.t1	AT3G55170	85.000	100	15	0	1	100	1	100	6.85e-53	161
MS.gene068553.t1	AT3G55170	85.000	100	15	0	1	100	1	100	6.85e-53	161
MS.gene068553.t1	AT3G55170	85.000	100	15	0	1	100	1	100	6.85e-53	161
MS.gene068553.t1	AT5G02610	71.311	122	12	1	1	99	1	122	4.42e-49	152

Find 19 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTGATAGCACGGGTCTTCTT+AGG	0.307833	1.2:+50933934	None:intergenic
TGCATTTGAAGTGAGAATTA+AGG	0.320554	1.2:-50934285	MS.gene068553:intron
CTGGTTCAGCAAATCAGCTT+TGG	0.322482	1.2:+50934241	None:intergenic
TGGTTCAGCAAATCAGCTTT+GGG	0.386643	1.2:+50934242	None:intergenic
AGGTGCACCGCCGGTGACTT+TGG	0.448506	1.2:+50934178	None:intergenic
ATTAAACCTGGTGTTTGGTA+AGG	0.478429	1.2:+50933905	None:intergenic
CCGCCTTCAGATCCTTCAGC+TGG	0.492136	1.2:+50934222	None:intergenic
CTTCGAGTCGCCAAAGTCAC+CGG	0.499663	1.2:-50934188	MS.gene068553:CDS
GAGCTTGTTAGGTGCACCGC+CGG	0.541755	1.2:+50934169	None:intergenic
GAATTAAGGTGTTCGAGTTG+AGG	0.547821	1.2:-50934271	MS.gene068553:CDS
TGATTTGCTGAACCAGCTGA+AGG	0.570604	1.2:-50934234	MS.gene068553:CDS
GAACCAGCTGAAGGATCTGA+AGG	0.580944	1.2:-50934225	MS.gene068553:CDS
TAAGGCGTCTTCTGATAGCA+CGG	0.582904	1.2:+50933923	None:intergenic
TTCTTAGGACGAAGATCAAG+TGG	0.602701	1.2:+50933949	None:intergenic
AAGGCGTCTTCTGATAGCAC+GGG	0.605672	1.2:+50933924	None:intergenic
AAGACGCCTTACCAAACACC+AGG	0.624051	1.2:-50933911	MS.gene068553:CDS
CGAGTCGCCAAAGTCACCGG+CGG	0.675842	1.2:-50934185	MS.gene068553:CDS
CCAGCTGAAGGATCTGAAGG+CGG	0.695519	1.2:-50934222	MS.gene068553:CDS
GCAGAAAGCTGCGCTCAGAG+AGG	0.696591	1.2:-50933995	MS.gene068553:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATACATAATCGCTTTTATA+TGG	-	chr1.2:50934152-50934171	MS.gene068553:intron	20.0%
!!!	TATGTATTGTTTACACATTT+TGG	+	chr1.2:50934140-50934159	None:intergenic	20.0%
	TTATATGGAAGCTGCTTATA+TGG	-	chr1.2:50934167-50934186	MS.gene068553:CDS	30.0%
!	TGCATTTGAAGTGAGAATTA+AGG	-	chr1.2:50933997-50934016	MS.gene068553:CDS	30.0%
!!!	TTTTTTACAGCAAAGTTGTG+AGG	-	chr1.2:50934226-50934245	MS.gene068553:CDS	30.0%
	AATTCTCACTTCAAATGCAG+TGG	+	chr1.2:50933996-50934015	None:intergenic	35.0%
!!	TGGTAATCGCGTTTTGTTAA+TGG	-	chr1.2:50934187-50934206	MS.gene068553:CDS	35.0%
	TGGTTCAGCAAATCAGCTTT+GGG	+	chr1.2:50934043-50934062	None:intergenic	40.0%
	TTCTTAGGACGAAGATCAAG+TGG	+	chr1.2:50934336-50934355	None:intergenic	40.0%
!!	CACATTTTGGAGAGCTTGTT+AGG	+	chr1.2:50934127-50934146	None:intergenic	40.0%
!!	GAATTAAGGTGTTCGAGTTG+AGG	-	chr1.2:50934011-50934030	MS.gene068553:CDS	40.0%
	CTGGTTCAGCAAATCAGCTT+TGG	+	chr1.2:50934044-50934063	None:intergenic	45.0%
!	TAAGGCGTCTTCTGATAGCA+CGG	+	chr1.2:50934362-50934381	None:intergenic	45.0%
!!	TGATTTGCTGAACCAGCTGA+AGG	-	chr1.2:50934048-50934067	MS.gene068553:CDS	45.0%
	AAGACGCCTTACCAAACACC+AGG	-	chr1.2:50934371-50934390	MS.gene068553:intron	50.0%
	CTGATAGCACGGGTCTTCTT+AGG	+	chr1.2:50934351-50934370	None:intergenic	50.0%
	GAACCAGCTGAAGGATCTGA+AGG	-	chr1.2:50934057-50934076	MS.gene068553:intron	50.0%
!	AAGGCGTCTTCTGATAGCAC+GGG	+	chr1.2:50934361-50934380	None:intergenic	50.0%
	CCAGCTGAAGGATCTGAAGG+CGG	-	chr1.2:50934060-50934079	MS.gene068553:intron	55.0%
	CTTCGAGTCGCCAAAGTCAC+CGG	-	chr1.2:50934094-50934113	MS.gene068553:intron	55.0%
	CCGCCTTCAGATCCTTCAGC+TGG	+	chr1.2:50934063-50934082	None:intergenic	60.0%
	GCAGAAAGCTGCGCTCAGAG+AGG	-	chr1.2:50934287-50934306	MS.gene068553:intron	60.0%
!!	GAGCTTGTTAGGTGCACCGC+CGG	+	chr1.2:50934116-50934135	None:intergenic	60.0%
	AGGTGCACCGCCGGTGACTT+TGG	+	chr1.2:50934107-50934126	None:intergenic	65.0%
	CGAGTCGCCAAAGTCACCGG+CGG	-	chr1.2:50934097-50934116	MS.gene068553:intron	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	50933906	50934398	50933906	ID=MS.gene068553
chr1.2	mRNA	50933906	50934398	50933906	ID=MS.gene068553.t1;Parent=MS.gene068553
chr1.2	exon	50934395	50934398	50934395	ID=MS.gene068553.t1.exon1;Parent=MS.gene068553.t1
chr1.2	CDS	50934395	50934398	50934395	ID=cds.MS.gene068553.t1;Parent=MS.gene068553.t1
chr1.2	exon	50934161	50934296	50934161	ID=MS.gene068553.t1.exon2;Parent=MS.gene068553.t1
chr1.2	CDS	50934161	50934296	50934161	ID=cds.MS.gene068553.t1;Parent=MS.gene068553.t1
chr1.2	exon	50933906	50934068	50933906	ID=MS.gene068553.t1.exon3;Parent=MS.gene068553.t1
chr1.2	CDS	50933906	50934068	50933906	ID=cds.MS.gene068553.t1;Parent=MS.gene068553.t1

Gene Sequence

>MS.gene068553

ATGGGTAAGAATGTGAATTTAGTTTCAGTAACGATTTAATCAAATCAAATTTGTAACATTTTTCAACATATAAGCTGAATTGAATTTCCACTGCATTTGAAGTGAGAATTAAGGTGTTCGAGTTGAGGAACAAACCCAAAGCTGATTTGCTGAACCAGCTGAAGGATCTGAAGGCGGAGCTCGCTTTGCTTCGAGTCGCCAAAGTCACCGGCGGTGCACCTAACAAGCTCTCCAAAATGTGTAAACAATACATAATCGCTTTTATATGGAAGCTGCTTATATGGTAATCGCGTTTTGTTAATGGTGTTTTTGTTTGTTTGTTTTTTACAGCAAAGTTGTGAGGCTTTCAATTGCACAAGTGTTGACAGTGATTTCACAGAAGCAGAAAGCTGCGCTCAGAGAGGTTTACAAAAATAAGAAGTATTTACCACTTGATCTTCGTCCTAAGAAGACCCGTGCTATCAGAAGACGCCTTACCAAACACCAGGTTTAA

Protein sequence

>MS.gene068553.t1

MVRIKVFELRNKPKADLLNQLKDLKAELALLRVAKVTGGAPNKLSKIKVVRLSIAQVLTVISQKQKAALREVYKNKKYLPLDLRPKKTRAIRRRLTKHQV