Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069266.t1 | XP_013444899.1 | 95 | 298 | 14 | 1 | 1 | 298 | 1 | 297 | 8.20E-156 | 559.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069266.t1 | Q9M2E2 | 61.1 | 301 | 109 | 6 | 1 | 298 | 1 | 296 | 5.5e-92 | 339.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069266.t1 | A0A072TN70 | 95.0 | 298 | 14 | 1 | 1 | 298 | 1 | 297 | 5.9e-156 | 559.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049170 | MS.gene069266 | 0.807558 | 4.77E-50 | -1.69E-46 |
| MS.gene049356 | MS.gene069266 | 0.844533 | 7.13E-59 | -1.69E-46 |
| MS.gene049369 | MS.gene069266 | 0.813616 | 2.34E-51 | -1.69E-46 |
| MS.gene049758 | MS.gene069266 | 0.831279 | 1.83E-55 | -1.69E-46 |
| MS.gene050060 | MS.gene069266 | 0.811209 | 7.85E-51 | -1.69E-46 |
| MS.gene050128 | MS.gene069266 | 0.820988 | 5.13E-53 | -1.69E-46 |
| MS.gene050301 | MS.gene069266 | 0.821128 | 4.76E-53 | -1.69E-46 |
| MS.gene050397 | MS.gene069266 | 0.803071 | 4.15E-49 | -1.69E-46 |
| MS.gene050432 | MS.gene069266 | 0.808618 | 2.84E-50 | -1.69E-46 |
| MS.gene050529 | MS.gene069266 | 0.815842 | 7.51E-52 | -1.69E-46 |
| MS.gene050670 | MS.gene069266 | 0.844779 | 6.12E-59 | -1.69E-46 |
| MS.gene050971 | MS.gene069266 | 0.812936 | 3.30E-51 | -1.69E-46 |
| MS.gene05105 | MS.gene069266 | 0.809213 | 2.11E-50 | -1.69E-46 |
| MS.gene051228 | MS.gene069266 | 0.810546 | 1.09E-50 | -1.69E-46 |
| MS.gene051471 | MS.gene069266 | 0.82027 | 7.49E-53 | -1.69E-46 |
| MS.gene052077 | MS.gene069266 | 0.820743 | 5.84E-53 | -1.69E-46 |
| MS.gene052078 | MS.gene069266 | 0.829747 | 4.33E-55 | -1.69E-46 |
| MS.gene052188 | MS.gene069266 | 0.885689 | 6.55E-72 | -1.69E-46 |
| MS.gene052253 | MS.gene069266 | 0.800925 | 1.15E-48 | -1.69E-46 |
| MS.gene052290 | MS.gene069266 | 0.821839 | 3.26E-53 | -1.69E-46 |
| MS.gene052310 | MS.gene069266 | 0.856858 | 2.42E-62 | -1.69E-46 |
| MS.gene052340 | MS.gene069266 | 0.87359 | 1.31E-67 | -1.69E-46 |
| MS.gene052421 | MS.gene069266 | 0.806773 | 6.99E-50 | -1.69E-46 |
| MS.gene052870 | MS.gene069266 | 0.807213 | 5.64E-50 | -1.69E-46 |
| MS.gene053073 | MS.gene069266 | 0.813389 | 2.62E-51 | -1.69E-46 |
| MS.gene053103 | MS.gene069266 | 0.832546 | 8.89E-56 | -1.69E-46 |
| MS.gene053104 | MS.gene069266 | 0.804646 | 1.95E-49 | -1.69E-46 |
| MS.gene05316 | MS.gene069266 | 0.847606 | 1.04E-59 | -1.69E-46 |
| MS.gene053209 | MS.gene069266 | 0.833127 | 6.38E-56 | -1.69E-46 |
| MS.gene053384 | MS.gene069266 | 0.839314 | 1.71E-57 | -1.69E-46 |
| MS.gene053449 | MS.gene069266 | 0.815658 | 8.26E-52 | -1.69E-46 |
| MS.gene053557 | MS.gene069266 | 0.83086 | 2.31E-55 | -1.69E-46 |
| MS.gene053836 | MS.gene069266 | 0.800447 | 1.43E-48 | -1.69E-46 |
| MS.gene053916 | MS.gene069266 | 0.800065 | 1.72E-48 | -1.69E-46 |
| MS.gene053928 | MS.gene069266 | 0.824601 | 7.39E-54 | -1.69E-46 |
| MS.gene054123 | MS.gene069266 | 0.860528 | 1.94E-63 | -1.69E-46 |
| MS.gene054349 | MS.gene069266 | 0.802068 | 6.69E-49 | -1.69E-46 |
| MS.gene054955 | MS.gene069266 | 0.824807 | 6.61E-54 | -1.69E-46 |
| MS.gene05505 | MS.gene069266 | 0.813037 | 3.13E-51 | -1.69E-46 |
| MS.gene055116 | MS.gene069266 | 0.805231 | 1.48E-49 | -1.69E-46 |
| MS.gene055277 | MS.gene069266 | 0.826131 | 3.21E-54 | -1.69E-46 |
| MS.gene055412 | MS.gene069266 | 0.801404 | 9.15E-49 | -1.69E-46 |
| MS.gene055422 | MS.gene069266 | 0.819811 | 9.55E-53 | -1.69E-46 |
| MS.gene05592 | MS.gene069266 | 0.829405 | 5.24E-55 | -1.69E-46 |
| MS.gene056407 | MS.gene069266 | 0.838463 | 2.83E-57 | -1.69E-46 |
| MS.gene056511 | MS.gene069266 | 0.82304 | 1.72E-53 | -1.69E-46 |
| MS.gene056609 | MS.gene069266 | 0.864162 | 1.48E-64 | -1.69E-46 |
| MS.gene056612 | MS.gene069266 | 0.815928 | 7.19E-52 | -1.69E-46 |
| MS.gene056659 | MS.gene069266 | 0.846591 | 1.97E-59 | -1.69E-46 |
| MS.gene056719 | MS.gene069266 | 0.885084 | 1.10E-71 | -1.69E-46 |
| MS.gene056897 | MS.gene069266 | 0.802483 | 5.49E-49 | -1.69E-46 |
| MS.gene05702 | MS.gene069266 | 0.806354 | 8.57E-50 | -1.69E-46 |
| MS.gene057124 | MS.gene069266 | 0.822188 | 2.71E-53 | -1.69E-46 |
| MS.gene05735 | MS.gene069266 | 0.858951 | 5.79E-63 | -1.69E-46 |
| MS.gene057396 | MS.gene069266 | 0.821774 | 3.38E-53 | -1.69E-46 |
| MS.gene057448 | MS.gene069266 | 0.822629 | 2.14E-53 | -1.69E-46 |
| MS.gene057449 | MS.gene069266 | 0.819949 | 8.88E-53 | -1.69E-46 |
| MS.gene057450 | MS.gene069266 | 0.836741 | 7.83E-57 | -1.69E-46 |
| MS.gene057537 | MS.gene069266 | 0.816413 | 5.60E-52 | -1.69E-46 |
| MS.gene057927 | MS.gene069266 | 0.850076 | 2.15E-60 | -1.69E-46 |
| MS.gene058007 | MS.gene069266 | 0.807498 | 4.91E-50 | -1.69E-46 |
| MS.gene058082 | MS.gene069266 | 0.854689 | 1.04E-61 | -1.69E-46 |
| MS.gene05811 | MS.gene069266 | 0.802714 | 4.92E-49 | -1.69E-46 |
| MS.gene058293 | MS.gene069266 | 0.837805 | 4.19E-57 | -1.69E-46 |
| MS.gene058365 | MS.gene069266 | 0.865342 | 6.33E-65 | -1.69E-46 |
| MS.gene058660 | MS.gene069266 | 0.816964 | 4.21E-52 | -1.69E-46 |
| MS.gene058928 | MS.gene069266 | 0.832905 | 7.24E-56 | -1.69E-46 |
| MS.gene05907 | MS.gene069266 | 0.81698 | 4.18E-52 | -1.69E-46 |
| MS.gene059285 | MS.gene069266 | 0.812828 | 3.48E-51 | -1.69E-46 |
| MS.gene059366 | MS.gene069266 | 0.816036 | 6.80E-52 | -1.69E-46 |
| MS.gene059597 | MS.gene069266 | 0.835391 | 1.72E-56 | -1.69E-46 |
| MS.gene05959 | MS.gene069266 | 0.856139 | 3.94E-62 | -1.69E-46 |
| MS.gene059674 | MS.gene069266 | 0.805326 | 1.41E-49 | -1.69E-46 |
| MS.gene059676 | MS.gene069266 | 0.870971 | 9.75E-67 | -1.69E-46 |
| MS.gene059688 | MS.gene069266 | 0.842114 | 3.15E-58 | -1.69E-46 |
| MS.gene059692 | MS.gene069266 | 0.856937 | 2.29E-62 | -1.69E-46 |
| MS.gene059695 | MS.gene069266 | 0.851169 | 1.06E-60 | -1.69E-46 |
| MS.gene059708 | MS.gene069266 | 0.818225 | 2.19E-52 | -1.69E-46 |
| MS.gene059711 | MS.gene069266 | 0.810984 | 8.78E-51 | -1.69E-46 |
| MS.gene059788 | MS.gene069266 | 0.8004 | 1.47E-48 | -1.69E-46 |
| MS.gene059807 | MS.gene069266 | 0.8248 | 6.63E-54 | -1.69E-46 |
| MS.gene05984 | MS.gene069266 | 0.81101 | 8.67E-51 | -1.69E-46 |
| MS.gene059894 | MS.gene069266 | 0.856403 | 3.29E-62 | -1.69E-46 |
| MS.gene059899 | MS.gene069266 | 0.8026 | 5.19E-49 | -1.69E-46 |
| MS.gene060407 | MS.gene069266 | 0.814723 | 1.33E-51 | -1.69E-46 |
| MS.gene060495 | MS.gene069266 | 0.808989 | 2.36E-50 | -1.69E-46 |
| MS.gene060496 | MS.gene069266 | 0.840175 | 1.02E-57 | -1.69E-46 |
| MS.gene060804 | MS.gene069266 | 0.814566 | 1.44E-51 | -1.69E-46 |
| MS.gene060818 | MS.gene069266 | 0.825252 | 5.19E-54 | -1.69E-46 |
| MS.gene061041 | MS.gene069266 | 0.82129 | 4.37E-53 | -1.69E-46 |
| MS.gene061049 | MS.gene069266 | 0.836606 | 8.48E-57 | -1.69E-46 |
| MS.gene061051 | MS.gene069266 | -0.863646 | 2.15E-64 | -1.69E-46 |
| MS.gene061154 | MS.gene069266 | 0.818605 | 1.80E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069266.t1 | MTR_8g031630 | 94.966 | 298 | 14 | 1 | 1 | 298 | 1 | 297 | 0.0 | 579 |
| MS.gene069266.t1 | MTR_8g031650 | 94.810 | 289 | 14 | 1 | 10 | 298 | 270 | 557 | 0.0 | 560 |
| MS.gene069266.t1 | MTR_8g031650 | 95.911 | 269 | 11 | 0 | 1 | 269 | 1 | 269 | 0.0 | 530 |
| MS.gene069266.t1 | MTR_8g031650 | 94.810 | 289 | 14 | 1 | 10 | 298 | 104 | 391 | 0.0 | 558 |
| MS.gene069266.t1 | MTR_8g031650 | 95.146 | 103 | 5 | 0 | 167 | 269 | 1 | 103 | 1.73e-66 | 213 |
| MS.gene069266.t1 | MTR_8g031600 | 89.632 | 299 | 29 | 2 | 1 | 298 | 1 | 298 | 0.0 | 539 |
| MS.gene069266.t1 | MTR_8g031610 | 91.753 | 291 | 22 | 2 | 8 | 298 | 6 | 294 | 0.0 | 538 |
| MS.gene069266.t1 | MTR_5g062280 | 71.477 | 298 | 82 | 3 | 1 | 298 | 1 | 295 | 1.43e-150 | 424 |
| MS.gene069266.t1 | MTR_7g076660 | 61.204 | 299 | 102 | 5 | 1 | 298 | 1 | 286 | 7.70e-126 | 360 |
| MS.gene069266.t1 | MTR_8g032010 | 59.333 | 300 | 118 | 3 | 1 | 298 | 1 | 298 | 5.65e-124 | 356 |
| MS.gene069266.t1 | MTR_8g031940 | 58.940 | 302 | 117 | 4 | 1 | 298 | 1 | 299 | 1.56e-123 | 355 |
| MS.gene069266.t1 | MTR_7g076655 | 58.863 | 299 | 109 | 5 | 1 | 298 | 1 | 286 | 1.73e-123 | 355 |
| MS.gene069266.t1 | MTR_8g031990 | 59.000 | 300 | 119 | 3 | 1 | 298 | 1 | 298 | 6.76e-123 | 353 |
| MS.gene069266.t1 | MTR_7g076660 | 60.751 | 293 | 101 | 5 | 7 | 298 | 8 | 287 | 1.26e-122 | 352 |
| MS.gene069266.t1 | MTR_8g031980 | 57.807 | 301 | 121 | 4 | 1 | 298 | 1 | 298 | 9.65e-121 | 348 |
| MS.gene069266.t1 | MTR_8g031960 | 58.763 | 291 | 114 | 4 | 7 | 294 | 8 | 295 | 1.21e-119 | 345 |
| MS.gene069266.t1 | MTR_8g032000 | 59.589 | 292 | 114 | 3 | 1 | 290 | 1 | 290 | 7.07e-119 | 343 |
| MS.gene069266.t1 | MTR_8g032020 | 57.191 | 299 | 123 | 3 | 3 | 298 | 4 | 300 | 8.98e-116 | 335 |
| MS.gene069266.t1 | MTR_8g031900 | 57.770 | 296 | 116 | 5 | 1 | 294 | 1 | 289 | 9.61e-116 | 335 |
| MS.gene069266.t1 | MTR_7g076665 | 57.333 | 300 | 121 | 5 | 1 | 298 | 1 | 295 | 2.76e-113 | 329 |
| MS.gene069266.t1 | MTR_8g031910 | 57.525 | 299 | 114 | 6 | 1 | 298 | 1 | 287 | 7.30e-113 | 328 |
| MS.gene069266.t1 | MTR_7g076660 | 60.219 | 274 | 95 | 5 | 26 | 298 | 2 | 262 | 1.97e-112 | 325 |
| MS.gene069266.t1 | MTR_7g106800 | 53.741 | 294 | 76 | 5 | 7 | 295 | 9 | 247 | 2.56e-95 | 282 |
| MS.gene069266.t1 | MTR_8g031850 | 48.980 | 294 | 107 | 4 | 1 | 294 | 1 | 251 | 2.02e-90 | 270 |
| MS.gene069266.t1 | MTR_3g079320 | 48.333 | 300 | 145 | 6 | 6 | 298 | 10 | 306 | 5.93e-86 | 260 |
| MS.gene069266.t1 | MTR_3g098470 | 40.864 | 301 | 141 | 8 | 9 | 298 | 37 | 311 | 1.41e-66 | 211 |
| MS.gene069266.t1 | MTR_1g031180 | 41.860 | 301 | 138 | 8 | 9 | 298 | 42 | 316 | 1.29e-65 | 208 |
| MS.gene069266.t1 | MTR_5g083430 | 49.761 | 209 | 80 | 3 | 90 | 298 | 1 | 184 | 1.10e-62 | 196 |
| MS.gene069266.t1 | MTR_8g032030 | 58.046 | 174 | 70 | 1 | 1 | 171 | 1 | 174 | 2.25e-60 | 192 |
| MS.gene069266.t1 | MTR_8g096060 | 37.874 | 301 | 147 | 9 | 9 | 298 | 39 | 310 | 9.93e-56 | 182 |
| MS.gene069266.t1 | MTR_4g087460 | 38.961 | 308 | 143 | 10 | 9 | 298 | 24 | 304 | 1.34e-54 | 180 |
| MS.gene069266.t1 | MTR_4g105310 | 26.070 | 257 | 132 | 8 | 9 | 258 | 51 | 256 | 2.85e-13 | 68.9 |
| MS.gene069266.t1 | MTR_4g105310 | 26.070 | 257 | 132 | 8 | 9 | 258 | 51 | 256 | 7.21e-13 | 68.6 |
| MS.gene069266.t1 | MTR_6g041980 | 26.370 | 292 | 167 | 9 | 9 | 297 | 69 | 315 | 4.95e-12 | 65.9 |
| MS.gene069266.t1 | MTR_6g041980 | 28.481 | 158 | 89 | 3 | 9 | 165 | 69 | 203 | 6.33e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069266.t1 | AT3G61220 | 61.258 | 302 | 107 | 6 | 1 | 298 | 1 | 296 | 5.05e-122 | 351 |
| MS.gene069266.t1 | AT3G61220 | 61.149 | 296 | 105 | 6 | 7 | 298 | 14 | 303 | 3.18e-119 | 344 |
| MS.gene069266.t1 | AT3G61220 | 61.149 | 296 | 105 | 6 | 7 | 298 | 38 | 327 | 1.15e-118 | 344 |
| MS.gene069266.t1 | AT1G01800 | 57.239 | 297 | 122 | 4 | 4 | 298 | 2 | 295 | 9.00e-113 | 328 |
| MS.gene069266.t1 | AT2G24190 | 55.814 | 301 | 125 | 6 | 1 | 298 | 1 | 296 | 3.13e-112 | 327 |
| MS.gene069266.t1 | AT2G24190 | 55.932 | 295 | 122 | 6 | 7 | 298 | 12 | 301 | 4.05e-110 | 321 |
| MS.gene069266.t1 | AT1G01800 | 56.459 | 209 | 88 | 3 | 90 | 298 | 55 | 260 | 7.81e-75 | 230 |
| MS.gene069266.t1 | AT5G51030 | 39.867 | 301 | 144 | 8 | 9 | 298 | 40 | 314 | 3.87e-64 | 204 |
| MS.gene069266.t1 | AT3G59710 | 43.151 | 292 | 136 | 9 | 9 | 296 | 21 | 286 | 4.24e-62 | 198 |
| MS.gene069266.t1 | AT3G59710 | 43.151 | 292 | 136 | 9 | 9 | 296 | 31 | 296 | 5.78e-62 | 198 |
| MS.gene069266.t1 | AT3G59710 | 43.151 | 292 | 136 | 9 | 9 | 296 | 50 | 315 | 1.72e-61 | 198 |
| MS.gene069266.t1 | AT5G61830 | 41.391 | 302 | 141 | 8 | 9 | 298 | 39 | 316 | 5.81e-61 | 196 |
| MS.gene069266.t1 | AT5G61830 | 41.391 | 302 | 141 | 8 | 9 | 298 | 39 | 316 | 5.81e-61 | 196 |
| MS.gene069266.t1 | AT4G09750 | 28.146 | 302 | 147 | 10 | 10 | 297 | 47 | 292 | 1.19e-14 | 73.6 |
| MS.gene069266.t1 | AT5G50600 | 32.919 | 161 | 86 | 4 | 10 | 170 | 51 | 189 | 1.14e-13 | 70.9 |
| MS.gene069266.t1 | AT5G50700 | 32.919 | 161 | 86 | 4 | 10 | 170 | 51 | 189 | 1.14e-13 | 70.9 |
Find 55 sgRNAs with CRISPR-Local
Find 116 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGAAACATTGACAACTTT+TGG | 0.257926 | 8.2:-66432606 | None:intergenic |
| CAATAAATCCTTGATCAAAA+TGG | 0.282362 | 8.2:-66433357 | None:intergenic |
| CTACCTGATGGTAGTCCTTC+TGG | 0.286806 | 8.2:+66433306 | MS.gene069266:CDS |
| TCTAGGTATGCAGTTGTTAC+AGG | 0.295664 | 8.2:+66431284 | MS.gene069266:intron |
| GTAAACAATGCTGGAATTCC+TGG | 0.304962 | 8.2:+66432070 | MS.gene069266:CDS |
| ATTTGGAAAACTAGATATCT+TGG | 0.368302 | 8.2:+66431514 | MS.gene069266:CDS |
| CAATGGCAGAAGCAACAAAA+AGG | 0.374057 | 8.2:+66431147 | None:intergenic |
| TATGTAGTCATACTATTAAT+AGG | 0.380899 | 8.2:-66433114 | None:intergenic |
| GCATATTGATGGATACTTCT+TGG | 0.385641 | 8.2:-66433181 | None:intergenic |
| ACAAGTGGACGGTGAGGCTT+TGG | 0.405380 | 8.2:+66432096 | MS.gene069266:CDS |
| ACCTAACTCAGTTTGTTCAT+AGG | 0.408762 | 8.2:-66432511 | None:intergenic |
| GGTATTAAAACAAACTACTA+TGG | 0.413302 | 8.2:+66432531 | MS.gene069266:CDS |
| AAAATGGTGGCCAGATTGAT+TGG | 0.424937 | 8.2:+66432469 | MS.gene069266:CDS |
| ATTTGCAGTATGCAAGCAAT+TGG | 0.426094 | 8.2:+66431325 | MS.gene069266:CDS |
| ACAGGAGCAAACAAAGGAAT+AGG | 0.427233 | 8.2:+66431302 | MS.gene069266:CDS |
| ACCTATGAACAAACTGAGTT+AGG | 0.431339 | 8.2:+66432510 | MS.gene069266:CDS |
| TCACCGTCCACTTGTGCTCC+AGG | 0.436898 | 8.2:-66432088 | None:intergenic |
| TTCTTCATCCATGGGAAAGT+TGG | 0.438371 | 8.2:+66432623 | MS.gene069266:CDS |
| TGTCAATGTTTCTTCATCCA+TGG | 0.442196 | 8.2:+66432614 | MS.gene069266:CDS |
| ACAGCATTGATGCATATTGA+TGG | 0.445089 | 8.2:-66433192 | None:intergenic |
| TGCATCAATGCTGTTTGTCC+TGG | 0.446484 | 8.2:+66433201 | MS.gene069266:CDS |
| ATTGATTCAGCACCTTCATC+AGG | 0.451708 | 8.2:-66433267 | None:intergenic |
| TGCAAGCAATTGGCTTCTAA+AGG | 0.461232 | 8.2:+66431335 | MS.gene069266:CDS |
| TTTGCTTAGGTAAACAATGC+TGG | 0.462288 | 8.2:+66432061 | MS.gene069266:intron |
| ACTTCCTTAGGCCATCCATT+TGG | 0.464515 | 8.2:-66432988 | None:intergenic |
| GTCAATGTTTCTTCATCCAT+GGG | 0.469153 | 8.2:+66432615 | MS.gene069266:CDS |
| TTGCTCTAAGTGCTTATACA+AGG | 0.490586 | 8.2:+66433151 | MS.gene069266:CDS |
| ACAGATATAAATTACAATAC+AGG | 0.491756 | 8.2:+66433234 | MS.gene069266:CDS |
| CTGAAACATCTTTCTCTGCC+TGG | 0.491788 | 8.2:+66431416 | MS.gene069266:CDS |
| TTCATCCATGGGAAAGTTGG+AGG | 0.493146 | 8.2:+66432626 | MS.gene069266:CDS |
| ACATCACTCAGTACTTCCTT+AGG | 0.505472 | 8.2:-66433000 | None:intergenic |
| GCAAGCAATTGGCTTCTAAA+GGG | 0.509794 | 8.2:+66431336 | MS.gene069266:CDS |
| AAGAAATCATACCACAGTAT+TGG | 0.519317 | 8.2:-66432125 | None:intergenic |
| TGCAGAAACTTCCAAATGGA+TGG | 0.529550 | 8.2:+66432977 | MS.gene069266:intron |
| GGTTATTTAACACCTGATGA+AGG | 0.538333 | 8.2:+66433255 | MS.gene069266:CDS |
| TGCTCTAAGTGCTTATACAA+GGG | 0.545903 | 8.2:+66433152 | MS.gene069266:CDS |
| AAGTGCTTCTGTCAAGTCCT+TGG | 0.549362 | 8.2:-66432556 | None:intergenic |
| TGTTTGCAGAAACTTCCAAA+TGG | 0.554795 | 8.2:+66432973 | MS.gene069266:intron |
| CAGTAGTAGAAAACAAAGGT+TGG | 0.558034 | 8.2:+66433091 | MS.gene069266:CDS |
| TGCTGAATCAATTGTGAGGT+TGG | 0.563985 | 8.2:+66433278 | MS.gene069266:CDS |
| ACAAATGATCTTATACTCGC+AGG | 0.570380 | 8.2:-66431473 | None:intergenic |
| ACTTCCAAATGGATGGCCTA+AGG | 0.577137 | 8.2:+66432984 | MS.gene069266:CDS |
| TGACCAGAAGGACTACCATC+AGG | 0.583917 | 8.2:-66433309 | None:intergenic |
| AACAAACTACTATGGAGCCA+AGG | 0.591502 | 8.2:+66432539 | MS.gene069266:CDS |
| ATATACCTCCAACTTTCCCA+TGG | 0.596721 | 8.2:-66432631 | None:intergenic |
| GAGCTTGAAAGCTGGAGAAG+TGG | 0.610128 | 8.2:-66432582 | None:intergenic |
| TGGAATTCCTGGAGCACAAG+TGG | 0.626468 | 8.2:+66432081 | MS.gene069266:CDS |
| ATTCCTGGAGCACAAGTGGA+CGG | 0.643422 | 8.2:+66432085 | MS.gene069266:CDS |
| AGGTTGGCATTGCTACCTGA+TGG | 0.648903 | 8.2:+66433294 | MS.gene069266:CDS |
| TTTGGCTGCTGCCAATACTG+TGG | 0.652336 | 8.2:+66432114 | MS.gene069266:CDS |
| GTTGTTACAGGAGCAAACAA+AGG | 0.652694 | 8.2:+66431296 | MS.gene069266:CDS |
| GGTTCAGTAGTAGAAAACAA+AGG | 0.658570 | 8.2:+66433087 | MS.gene069266:CDS |
| TGGAGCACAAGTGGACGGTG+AGG | 0.671657 | 8.2:+66432090 | MS.gene069266:CDS |
| ACTGCAAGAGATGAAAAGAG+AGG | 0.684617 | 8.2:+66431374 | MS.gene069266:CDS |
| AAGGTGCTGAATCAATTGTG+AGG | 0.744935 | 8.2:+66433274 | MS.gene069266:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGAAAAATATCTAAAAAA+AGG | + | chr8.2:66432706-66432725 | MS.gene069266:intron | 10.0% |
| !! | AATTATAAGCTATTATTATA+AGG | + | chr8.2:66431972-66431991 | MS.gene069266:intron | 10.0% |
| !! | ATTTAATTGCATATATATGT+AGG | - | chr8.2:66431213-66431232 | None:intergenic | 15.0% |
| !! | TTTAATTGCATATATATGTA+GGG | - | chr8.2:66431212-66431231 | None:intergenic | 15.0% |
| !!! | TGAAGCATTAATAAATTTTA+AGG | - | chr8.2:66432872-66432891 | None:intergenic | 15.0% |
| !! | ACAGATATAAATTACAATAC+AGG | + | chr8.2:66433234-66433253 | MS.gene069266:CDS | 20.0% |
| !! | TATGTAGTCATACTATTAAT+AGG | - | chr8.2:66433117-66433136 | None:intergenic | 20.0% |
| !!! | AATTCAAGTTTTCTTGTTTT+TGG | + | chr8.2:66432439-66432458 | MS.gene069266:intron | 20.0% |
| !!! | AGATTTAAAATTGTTTTTGG+TGG | + | chr8.2:66431698-66431717 | MS.gene069266:intron | 20.0% |
| !!! | AGTTTTGAATCAATTTCTAA+AGG | + | chr8.2:66433053-66433072 | MS.gene069266:CDS | 20.0% |
| !!! | ATTTCTAAAGGATTTTAAAG+AGG | + | chr8.2:66433065-66433084 | MS.gene069266:CDS | 20.0% |
| !!! | GATAGAGTATATCTAAATTT+TGG | + | chr8.2:66431578-66431597 | MS.gene069266:intron | 20.0% |
| !!! | GTATATCTAAATTTTGGATT+TGG | + | chr8.2:66431584-66431603 | MS.gene069266:intron | 20.0% |
| !!! | TGCAGATTTAAAATTGTTTT+TGG | + | chr8.2:66431695-66431714 | MS.gene069266:intron | 20.0% |
| !!! | TTGAAATTAATTGATTTGCA+TGG | - | chr8.2:66432200-66432219 | None:intergenic | 20.0% |
| !!! | TTTCAGTTTTTTTAGTTGTT+TGG | - | chr8.2:66432346-66432365 | None:intergenic | 20.0% |
| !!! | TTTCTAAAGGATTTTAAAGA+GGG | + | chr8.2:66433066-66433085 | MS.gene069266:CDS | 20.0% |
| !!! | TTTTGAAGAATAAAAGGATA+AGG | - | chr8.2:66431187-66431206 | None:intergenic | 20.0% |
| ! | ATTTGGAAAACTAGATATCT+TGG | + | chr8.2:66431514-66431533 | MS.gene069266:CDS | 25.0% |
| ! | GGTATTAAAACAAACTACTA+TGG | + | chr8.2:66432531-66432550 | MS.gene069266:CDS | 25.0% |
| !! | ACTTTGAACTATTTTGCAAT+TGG | + | chr8.2:66432924-66432943 | MS.gene069266:intron | 25.0% |
| !! | ATAACTTTTGCAACTTTCTT+AGG | + | chr8.2:66432661-66432680 | MS.gene069266:intron | 25.0% |
| !! | CCAAAATATTTCTTTTGACA+AGG | - | chr8.2:66431944-66431963 | None:intergenic | 25.0% |
| !! | CCTTGTCAAAAGAAATATTT+TGG | + | chr8.2:66431941-66431960 | MS.gene069266:intron | 25.0% |
| !! | CTATTGTTATGATTTGTGAT+TGG | - | chr8.2:66431844-66431863 | None:intergenic | 25.0% |
| !! | GATTTTATCAAGAACCAATT+TGG | + | chr8.2:66431497-66431516 | MS.gene069266:CDS | 25.0% |
| !!! | ACAACAAAGGATTTTTAAGA+AGG | - | chr8.2:66432807-66432826 | None:intergenic | 25.0% |
| !!! | ACGTTGTTAATCTTTTTTCT+AGG | + | chr8.2:66431267-66431286 | MS.gene069266:intron | 25.0% |
| !!! | AGATATCTAGTTTTCCAAAT+TGG | - | chr8.2:66431514-66431533 | None:intergenic | 25.0% |
| !!! | AGGGTATTTTGAAGAATAAA+AGG | - | chr8.2:66431193-66431212 | None:intergenic | 25.0% |
| !!! | ATCTGTTTTTTCATACAAAG+TGG | - | chr8.2:66432376-66432395 | None:intergenic | 25.0% |
| !!! | ATTAGTTACGTTAGTTTTCA+GGG | + | chr8.2:66432765-66432784 | MS.gene069266:intron | 25.0% |
| !!! | TATTAGTTACGTTAGTTTTC+AGG | + | chr8.2:66432764-66432783 | MS.gene069266:intron | 25.0% |
| !!! | TTATTTTACTCCAATCAATC+TGG | - | chr8.2:66432482-66432501 | None:intergenic | 25.0% |
| AAGAAATCATACCACAGTAT+TGG | - | chr8.2:66432128-66432147 | None:intergenic | 30.0% | |
| AATCTGCATAATCAATCACT+TGG | - | chr8.2:66431683-66431702 | None:intergenic | 30.0% | |
| ACAATGCATTCGAATATCAA+CGG | - | chr8.2:66431873-66431892 | None:intergenic | 30.0% | |
| ATCGATTGAAGTAATAACCA+AGG | - | chr8.2:66432292-66432311 | None:intergenic | 30.0% | |
| ATCTGCATAATCAATCACTT+GGG | - | chr8.2:66431682-66431701 | None:intergenic | 30.0% | |
| CAAATATTTGTGATCATTCG+AGG | - | chr8.2:66432030-66432049 | None:intergenic | 30.0% | |
| TTGTTTACCTAAGCAAAGTT+TGG | - | chr8.2:66432058-66432077 | None:intergenic | 30.0% | |
| ! | CATCAATCTTTTCTTCTGTA+AGG | - | chr8.2:66433032-66433051 | None:intergenic | 30.0% |
| ! | GAAGAAACATTGACAACTTT+TGG | - | chr8.2:66432609-66432628 | None:intergenic | 30.0% |
| !! | ATTTTTAAGAAGGATGTCGA+GGG | - | chr8.2:66432797-66432816 | None:intergenic | 30.0% |
| !!! | GTTTTCTTGTTTTTGGTTTC+AGG | + | chr8.2:66432446-66432465 | MS.gene069266:intron | 30.0% |
| !!! | TGTTTTTGGTTTCAGGAAAA+TGG | + | chr8.2:66432453-66432472 | MS.gene069266:intron | 30.0% |
| ACAAATGATCTTATACTCGC+AGG | - | chr8.2:66431476-66431495 | None:intergenic | 35.0% | |
| ACCTATGAACAAACTGAGTT+AGG | + | chr8.2:66432510-66432529 | MS.gene069266:CDS | 35.0% | |
| ATTTGCAGTATGCAAGCAAT+TGG | + | chr8.2:66431325-66431344 | MS.gene069266:CDS | 35.0% | |
| CACTGAAGTAAGTGCATATT+TGG | + | chr8.2:66431638-66431657 | MS.gene069266:intron | 35.0% | |
| CAGTAGTAGAAAACAAAGGT+TGG | + | chr8.2:66433091-66433110 | MS.gene069266:CDS | 35.0% | |
| CTAACAAAAAAGTGACCAGA+AGG | - | chr8.2:66433324-66433343 | None:intergenic | 35.0% | |
| GACAAGGTATATCAACTCAA+TGG | - | chr8.2:66431928-66431947 | None:intergenic | 35.0% | |
| GGTTATTTAACACCTGATGA+AGG | + | chr8.2:66433255-66433274 | MS.gene069266:CDS | 35.0% | |
| GGTTCAGTAGTAGAAAACAA+AGG | + | chr8.2:66433087-66433106 | MS.gene069266:CDS | 35.0% | |
| GTCAATGTTTCTTCATCCAT+GGG | + | chr8.2:66432615-66432634 | MS.gene069266:CDS | 35.0% | |
| TGAAACTCCAAACTTTGCTT+AGG | + | chr8.2:66432048-66432067 | MS.gene069266:intron | 35.0% | |
| TGATGAAAAACAACAAGACC+AGG | - | chr8.2:66431437-66431456 | None:intergenic | 35.0% | |
| TGTCAATGTTTCTTCATCCA+TGG | + | chr8.2:66432614-66432633 | MS.gene069266:CDS | 35.0% | |
| TGTTTGCAGAAACTTCCAAA+TGG | + | chr8.2:66432973-66432992 | MS.gene069266:intron | 35.0% | |
| TTTGCTTAGGTAAACAATGC+TGG | + | chr8.2:66432061-66432080 | MS.gene069266:intron | 35.0% | |
| ! | ACCTAACTCAGTTTGTTCAT+AGG | - | chr8.2:66432514-66432533 | None:intergenic | 35.0% |
| ! | ATATCTGTTTTCACAAAGCC+AGG | - | chr8.2:66433222-66433241 | None:intergenic | 35.0% |
| ! | GCATATTGATGGATACTTCT+TGG | - | chr8.2:66433184-66433203 | None:intergenic | 35.0% |
| ! | TCTAAAGCGACTTAAAACCT+TGG | + | chr8.2:66432272-66432291 | MS.gene069266:intron | 35.0% |
| ! | TGCTCTAAGTGCTTATACAA+GGG | + | chr8.2:66433152-66433171 | MS.gene069266:CDS | 35.0% |
| ! | TTGCTCTAAGTGCTTATACA+AGG | + | chr8.2:66433151-66433170 | MS.gene069266:CDS | 35.0% |
| !! | ACAGCATTGATGCATATTGA+TGG | - | chr8.2:66433195-66433214 | None:intergenic | 35.0% |
| !! | GATTTTTAAGAAGGATGTCG+AGG | - | chr8.2:66432798-66432817 | None:intergenic | 35.0% |
| !! | GTTAAACTGCCTTAGCTTTT+CGG | - | chr8.2:66432154-66432173 | None:intergenic | 35.0% |
| !! | TTAAACTGCCTTAGCTTTTC+GGG | - | chr8.2:66432153-66432172 | None:intergenic | 35.0% |
| !! | TTTGGACTCAATTTTGAGAG+AGG | + | chr8.2:66431656-66431675 | MS.gene069266:intron | 35.0% |
| !!! | TTTTGGTTTCAGGAAAATGG+TGG | + | chr8.2:66432456-66432475 | MS.gene069266:intron | 35.0% |
| AAAATGGTGGCCAGATTGAT+TGG | + | chr8.2:66432469-66432488 | MS.gene069266:CDS | 40.0% | |
| AACAAACTACTATGGAGCCA+AGG | + | chr8.2:66432539-66432558 | MS.gene069266:CDS | 40.0% | |
| ACAGGAGCAAACAAAGGAAT+AGG | + | chr8.2:66431302-66431321 | MS.gene069266:CDS | 40.0% | |
| ACATCACTCAGTACTTCCTT+AGG | - | chr8.2:66433003-66433022 | None:intergenic | 40.0% | |
| ACTGCAAGAGATGAAAAGAG+AGG | + | chr8.2:66431374-66431393 | MS.gene069266:CDS | 40.0% | |
| AGTGAGCTAAAGGACAACAA+AGG | - | chr8.2:66432820-66432839 | None:intergenic | 40.0% | |
| ATATACCTCCAACTTTCCCA+TGG | - | chr8.2:66432634-66432653 | None:intergenic | 40.0% | |
| CACAAAGATTGACGTTGTTG+CGG | - | chr8.2:66432238-66432257 | None:intergenic | 40.0% | |
| GTAAACAATGCTGGAATTCC+TGG | + | chr8.2:66432070-66432089 | MS.gene069266:CDS | 40.0% | |
| GTTGTTACAGGAGCAAACAA+AGG | + | chr8.2:66431296-66431315 | MS.gene069266:CDS | 40.0% | |
| TCTAGGTATGCAGTTGTTAC+AGG | + | chr8.2:66431284-66431303 | MS.gene069266:intron | 40.0% | |
| TGCAGAAACTTCCAAATGGA+TGG | + | chr8.2:66432977-66432996 | MS.gene069266:intron | 40.0% | |
| TGCTGAATCAATTGTGAGGT+TGG | + | chr8.2:66433278-66433297 | MS.gene069266:CDS | 40.0% | |
| TTCTTCATCCATGGGAAAGT+TGG | + | chr8.2:66432623-66432642 | MS.gene069266:CDS | 40.0% | |
| TTTCTTTCCCCGAAAAGCTA+AGG | + | chr8.2:66432142-66432161 | MS.gene069266:intron | 40.0% | |
| ! | ATTGATTCAGCACCTTCATC+AGG | - | chr8.2:66433270-66433289 | None:intergenic | 40.0% |
| ! | GCAAGCAATTGGCTTCTAAA+GGG | + | chr8.2:66431336-66431355 | MS.gene069266:CDS | 40.0% |
| ! | TGCAAGCAATTGGCTTCTAA+AGG | + | chr8.2:66431335-66431354 | MS.gene069266:CDS | 40.0% |
| !! | AAGGTGCTGAATCAATTGTG+AGG | + | chr8.2:66433274-66433293 | MS.gene069266:CDS | 40.0% |
| !! | TAAACTGCCTTAGCTTTTCG+GGG | - | chr8.2:66432152-66432171 | None:intergenic | 40.0% |
| AAGTGCTTCTGTCAAGTCCT+TGG | - | chr8.2:66432559-66432578 | None:intergenic | 45.0% | |
| ACTTCCAAATGGATGGCCTA+AGG | + | chr8.2:66432984-66433003 | MS.gene069266:CDS | 45.0% | |
| ACTTCCTTAGGCCATCCATT+TGG | - | chr8.2:66432991-66433010 | None:intergenic | 45.0% | |
| CTGAAACATCTTTCTCTGCC+TGG | + | chr8.2:66431416-66431435 | MS.gene069266:CDS | 45.0% | |
| TTCATCCATGGGAAAGTTGG+AGG | + | chr8.2:66432626-66432645 | MS.gene069266:CDS | 45.0% | |
| ! | CTTTTGGTGAGCTTGAAAGC+TGG | - | chr8.2:66432593-66432612 | None:intergenic | 45.0% |
| ! | GAGATTCGAGTTCAAACCCT+CGG | - | chr8.2:66432850-66432869 | None:intergenic | 45.0% |
| ! | TGCATCAATGCTGTTTGTCC+TGG | + | chr8.2:66433201-66433220 | MS.gene069266:CDS | 45.0% |
| AGGTTGGCATTGCTACCTGA+TGG | + | chr8.2:66433294-66433313 | MS.gene069266:CDS | 50.0% | |
| ATTCCTGGAGCACAAGTGGA+CGG | + | chr8.2:66432085-66432104 | MS.gene069266:CDS | 50.0% | |
| CTACCTGATGGTAGTCCTTC+TGG | + | chr8.2:66433306-66433325 | MS.gene069266:CDS | 50.0% | |
| GAGCTTGAAAGCTGGAGAAG+TGG | - | chr8.2:66432585-66432604 | None:intergenic | 50.0% | |
| TGACCAGAAGGACTACCATC+AGG | - | chr8.2:66433312-66433331 | None:intergenic | 50.0% | |
| TGGAATTCCTGGAGCACAAG+TGG | + | chr8.2:66432081-66432100 | MS.gene069266:CDS | 50.0% | |
| TTTGGCTGCTGCCAATACTG+TGG | + | chr8.2:66432114-66432133 | MS.gene069266:CDS | 50.0% | |
| !! | GTCGCTTTAGAAGACGCAGT+TGG | - | chr8.2:66432263-66432282 | None:intergenic | 50.0% |
| ACAAGTGGACGGTGAGGCTT+TGG | + | chr8.2:66432096-66432115 | MS.gene069266:CDS | 55.0% | |
| TAGCTCACTAACCGAGCCGA+GGG | + | chr8.2:66432831-66432850 | MS.gene069266:intron | 55.0% | |
| TCGAGTTCAAACCCTCGGCT+CGG | - | chr8.2:66432845-66432864 | None:intergenic | 55.0% | |
| TTAGCTCACTAACCGAGCCG+AGG | + | chr8.2:66432830-66432849 | MS.gene069266:intron | 55.0% | |
| !! | CGGCTCGGTTAGTGAGCTAA+AGG | - | chr8.2:66432830-66432849 | None:intergenic | 55.0% |
| TCACCGTCCACTTGTGCTCC+AGG | - | chr8.2:66432091-66432110 | None:intergenic | 60.0% | |
| TGGAGCACAAGTGGACGGTG+AGG | + | chr8.2:66432090-66432109 | MS.gene069266:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 66431149 | 66433365 | 66431149 | ID=MS.gene069266 |
| chr8.2 | mRNA | 66431149 | 66433365 | 66431149 | ID=MS.gene069266.t1;Parent=MS.gene069266 |
| chr8.2 | exon | 66431149 | 66431168 | 66431149 | ID=MS.gene069266.t1.exon1;Parent=MS.gene069266.t1 |
| chr8.2 | CDS | 66431149 | 66431168 | 66431149 | ID=cds.MS.gene069266.t1;Parent=MS.gene069266.t1 |
| chr8.2 | exon | 66431289 | 66431535 | 66431289 | ID=MS.gene069266.t1.exon2;Parent=MS.gene069266.t1 |
| chr8.2 | CDS | 66431289 | 66431535 | 66431289 | ID=cds.MS.gene069266.t1;Parent=MS.gene069266.t1 |
| chr8.2 | exon | 66432070 | 66432135 | 66432070 | ID=MS.gene069266.t1.exon3;Parent=MS.gene069266.t1 |
| chr8.2 | CDS | 66432070 | 66432135 | 66432070 | ID=cds.MS.gene069266.t1;Parent=MS.gene069266.t1 |
| chr8.2 | exon | 66432468 | 66432647 | 66432468 | ID=MS.gene069266.t1.exon4;Parent=MS.gene069266.t1 |
| chr8.2 | CDS | 66432468 | 66432647 | 66432468 | ID=cds.MS.gene069266.t1;Parent=MS.gene069266.t1 |
| chr8.2 | exon | 66432982 | 66433365 | 66432982 | ID=MS.gene069266.t1.exon5;Parent=MS.gene069266.t1 |
| chr8.2 | CDS | 66432982 | 66433365 | 66432982 | ID=cds.MS.gene069266.t1;Parent=MS.gene069266.t1 |
| Gene Sequence |
| Protein sequence |
