AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070697


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070697.t1 MTR_1g032560 94.186 172 10 0 1 172 1 172 4.45e-112 316
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070697.t1 AT2G01755 58.475 118 49 0 54 171 56 173 1.58e-42 140
MS.gene070697.t1 AT2G01755 58.475 118 49 0 54 171 62 179 1.97e-42 140

Find 46 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGTCTATAGATAGAATAAA+AGG 0.213144 1.4:-17091311 MS.gene070697:CDS
ATCAAAGAGGTATAAGCTTT+TGG 0.314010 1.4:-17091822 MS.gene070697:CDS
TAAGTACTTCTACTTCAATT+TGG 0.331886 1.4:+17091656 None:intergenic
GGAAACTTGTCAATTGATAA+TGG 0.335882 1.4:+17091895 None:intergenic
CCATGGCATCATTGTCCATT+TGG 0.363669 1.4:+17091933 None:intergenic
AGAATGCTTCTTGTTGGGTC+AGG 0.383377 1.4:+17091353 None:intergenic
GAAGATAGAGATGATGAGTA+TGG 0.389990 1.4:-17091760 MS.gene070697:CDS
GCTGGAAGAATGCTTCTTGT+TGG 0.394629 1.4:+17091347 None:intergenic
CTTCTGCAGATACTCAATAT+TGG 0.406034 1.4:+17091274 None:intergenic
CTGGAAGAATGCTTCTTGTT+GGG 0.406470 1.4:+17091348 None:intergenic
GACTTTATCACTGCCCTGGC+TGG 0.412026 1.4:+17091329 None:intergenic
TTGTCAATTGATAATGGTAT+AGG 0.420243 1.4:+17091901 None:intergenic
AGTAGAAGTACTTATCTTCA+AGG 0.426944 1.4:-17091647 MS.gene070697:intron
TGTCCATTTGGCCATACCCT+TGG 0.430122 1.4:+17091945 None:intergenic
TCAATATTGGCAGGGTCAAA+AGG 0.439860 1.4:+17091287 None:intergenic
GTCCATTTGGCCATACCCTT+GGG 0.442034 1.4:+17091946 None:intergenic
CAATGAGGAAATTTCAAAGT+TGG 0.445765 1.4:-17091734 MS.gene070697:CDS
CTCTATCTTCATCCTCATCT+TGG 0.448503 1.4:+17091773 None:intergenic
TGCAGATACTCAATATTGGC+AGG 0.450870 1.4:+17091278 None:intergenic
TTCCCAAGGGTATGGCCAAA+TGG 0.469349 1.4:-17091948 MS.gene070697:CDS
ATACCCTTGGGAAGTGTAGT+AGG 0.478990 1.4:+17091958 None:intergenic
GGACAATGATGCCATGGCCT+AGG 0.481592 1.4:-17091927 MS.gene070697:CDS
CAAGAAGCATTCTTCCAGCC+AGG 0.483504 1.4:-17091343 MS.gene070697:CDS
GAATGCTTCTTGTTGGGTCA+GGG 0.497042 1.4:+17091354 None:intergenic
CACAAGCTTACATTGTGAGT+TGG 0.506810 1.4:+17091874 None:intergenic
AGTCTATAGATAGAATAAAA+GGG 0.523947 1.4:-17091310 MS.gene070697:CDS
AATGCTTCTTGTTGGGTCAG+GGG 0.544161 1.4:+17091355 None:intergenic
TACTACACTTCCCAAGGGTA+TGG 0.545556 1.4:-17091956 MS.gene070697:CDS
TTTGTCTTGAATTCTTCCCA+TGG 0.554335 1.4:+17091245 None:intergenic
ATCTGCAGAAGGATGTTCCA+TGG 0.555118 1.4:-17091262 MS.gene070697:CDS
GATAATGGTATAGGTAGCCT+AGG 0.559854 1.4:+17091910 None:intergenic
TATAGACTTTATCACTGCCC+TGG 0.571801 1.4:+17091325 None:intergenic
ACTCATTGTTCATGCACTTG+TGG 0.576626 1.4:-17091680 MS.gene070697:CDS
GCATCCTACTACACTTCCCA+AGG 0.594031 1.4:-17091962 MS.gene070697:CDS
AAGAAGCATTCTTCCAGCCA+GGG 0.602074 1.4:-17091342 MS.gene070697:CDS
TTCTATAGTCAATCAGCAAG+AGG 0.607447 1.4:-17091709 MS.gene070697:CDS
AGGGGAAGTACCATAGCTCA+AGG 0.608247 1.4:+17091373 None:intergenic
TCTGCAGAAGGATGTTCCAT+GGG 0.613447 1.4:-17091261 MS.gene070697:CDS
CAATATTGAGTATCTGCAGA+AGG 0.617485 1.4:-17091273 MS.gene070697:CDS
CATCCTACTACACTTCCCAA+GGG 0.625588 1.4:-17091961 MS.gene070697:CDS
CCAAATGGACAATGATGCCA+TGG 0.626699 1.4:-17091933 MS.gene070697:CDS
GGTATAGGTAGCCTAGGCCA+TGG 0.632885 1.4:+17091916 None:intergenic
GCAAATTAGATACCAAGATG+AGG 0.635629 1.4:-17091785 MS.gene070697:CDS
TTAGATTTGAGCAATCAAAG+AGG 0.645074 1.4:-17091835 MS.gene070697:CDS
GCAGATACTCAATATTGGCA+GGG 0.648369 1.4:+17091279 None:intergenic
TGATGAGTATGGACACAATG+AGG 0.651393 1.4:-17091749 MS.gene070697:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATAATTTGTTTGTTTTAT+TGG + chr1.4:17091625-17091644 None:intergenic 10.0%
!!! TTTTTTTTCTAATTAGAAAA+AGG - chr1.4:17091666-17091685 MS.gene070697:CDS 10.0%
!! AAGTCTATAGATAGAATAAA+AGG - chr1.4:17091882-17091901 MS.gene070697:CDS 20.0%
!! AGTCTATAGATAGAATAAAA+GGG - chr1.4:17091883-17091902 MS.gene070697:CDS 20.0%
!!! TTGTTTGTTTTATTGGTTAT+TGG + chr1.4:17091618-17091637 None:intergenic 20.0%
! CCATTTGCAAAACAAAAATT+TGG + chr1.4:17091723-17091742 None:intergenic 25.0%
! TAAGTACTTCTACTTCAATT+TGG + chr1.4:17091540-17091559 None:intergenic 25.0%
!!! CCAAATTTTTGTTTTGCAAA+TGG - chr1.4:17091720-17091739 MS.gene070697:CDS 25.0%
!!! TTGTCAATTGATAATGGTAT+AGG + chr1.4:17091295-17091314 None:intergenic 25.0%
ACTTATCTTCAAGGTAAGTT+TGG - chr1.4:17091555-17091574 MS.gene070697:intron 30.0%
CAATGAGGAAATTTCAAAGT+TGG - chr1.4:17091459-17091478 MS.gene070697:intron 30.0%
GGAAACTTGTCAATTGATAA+TGG + chr1.4:17091301-17091320 None:intergenic 30.0%
TGACAAACCATTTCATTTCA+GGG - chr1.4:17091786-17091805 MS.gene070697:CDS 30.0%
TTAGATTTGAGCAATCAAAG+AGG - chr1.4:17091358-17091377 MS.gene070697:CDS 30.0%
!! AGTAGAAGTACTTATCTTCA+AGG - chr1.4:17091546-17091565 MS.gene070697:intron 30.0%
!! ATCAAAGAGGTATAAGCTTT+TGG - chr1.4:17091371-17091390 MS.gene070697:CDS 30.0%
AGAAAACCCCTGAAATGAAA+TGG + chr1.4:17091796-17091815 None:intergenic 35.0%
CAATATTGAGTATCTGCAGA+AGG - chr1.4:17091920-17091939 MS.gene070697:CDS 35.0%
CTTCTGCAGATACTCAATAT+TGG + chr1.4:17091922-17091941 None:intergenic 35.0%
GAAGATAGAGATGATGAGTA+TGG - chr1.4:17091433-17091452 MS.gene070697:intron 35.0%
GACAAACCATTTCATTTCAG+GGG - chr1.4:17091787-17091806 MS.gene070697:CDS 35.0%
GCAAATTAGATACCAAGATG+AGG - chr1.4:17091408-17091427 MS.gene070697:intron 35.0%
GTGACAAACCATTTCATTTC+AGG - chr1.4:17091785-17091804 MS.gene070697:CDS 35.0%
TTCTATAGTCAATCAGCAAG+AGG - chr1.4:17091484-17091503 MS.gene070697:intron 35.0%
TTTGTCTTGAATTCTTCCCA+TGG + chr1.4:17091951-17091970 None:intergenic 35.0%
! TTTTCTTCATCCTTGAGCTA+TGG - chr1.4:17091810-17091829 MS.gene070697:CDS 35.0%
ACTCATTGTTCATGCACTTG+TGG - chr1.4:17091513-17091532 MS.gene070697:intron 40.0%
CACAAGCTTACATTGTGAGT+TGG + chr1.4:17091322-17091341 None:intergenic 40.0%
CTCTATCTTCATCCTCATCT+TGG + chr1.4:17091423-17091442 None:intergenic 40.0%
CTGGAAGAATGCTTCTTGTT+GGG + chr1.4:17091848-17091867 None:intergenic 40.0%
GCAGATACTCAATATTGGCA+GGG + chr1.4:17091917-17091936 None:intergenic 40.0%
TATAGACTTTATCACTGCCC+TGG + chr1.4:17091871-17091890 None:intergenic 40.0%
TCAATATTGGCAGGGTCAAA+AGG + chr1.4:17091909-17091928 None:intergenic 40.0%
TGATGAGTATGGACACAATG+AGG - chr1.4:17091444-17091463 MS.gene070697:intron 40.0%
TGCAGATACTCAATATTGGC+AGG + chr1.4:17091918-17091937 None:intergenic 40.0%
! GATAATGGTATAGGTAGCCT+AGG + chr1.4:17091286-17091305 None:intergenic 40.0%
!! AGAGGTATAAGCTTTTGGTG+TGG - chr1.4:17091376-17091395 MS.gene070697:CDS 40.0%
AATGCTTCTTGTTGGGTCAG+GGG + chr1.4:17091841-17091860 None:intergenic 45.0%
AGAATGCTTCTTGTTGGGTC+AGG + chr1.4:17091843-17091862 None:intergenic 45.0%
ATCTGCAGAAGGATGTTCCA+TGG - chr1.4:17091931-17091950 MS.gene070697:CDS 45.0%
CATCCTACTACACTTCCCAA+GGG - chr1.4:17091232-17091251 MS.gene070697:CDS 45.0%
CCAAATGGACAATGATGCCA+TGG - chr1.4:17091260-17091279 MS.gene070697:CDS 45.0%
CCATGGCATCATTGTCCATT+TGG + chr1.4:17091263-17091282 None:intergenic 45.0%
GAATGCTTCTTGTTGGGTCA+GGG + chr1.4:17091842-17091861 None:intergenic 45.0%
GCTGGAAGAATGCTTCTTGT+TGG + chr1.4:17091849-17091868 None:intergenic 45.0%
TACTACACTTCCCAAGGGTA+TGG - chr1.4:17091237-17091256 MS.gene070697:CDS 45.0%
TCTGCAGAAGGATGTTCCAT+GGG - chr1.4:17091932-17091951 MS.gene070697:CDS 45.0%
! ATACCCTTGGGAAGTGTAGT+AGG + chr1.4:17091238-17091257 None:intergenic 45.0%
!! AAGAAGCATTCTTCCAGCCA+GGG - chr1.4:17091851-17091870 MS.gene070697:CDS 45.0%
!! TAAGCTTTTGGTGTGGAGCA+AGG - chr1.4:17091383-17091402 MS.gene070697:CDS 45.0%
GCATCCTACTACACTTCCCA+AGG - chr1.4:17091231-17091250 MS.gene070697:CDS 50.0%
GTCCATTTGGCCATACCCTT+GGG + chr1.4:17091250-17091269 None:intergenic 50.0%
TGTCCATTTGGCCATACCCT+TGG + chr1.4:17091251-17091270 None:intergenic 50.0%
! TTCCCAAGGGTATGGCCAAA+TGG - chr1.4:17091245-17091264 MS.gene070697:CDS 50.0%
!! AGGGGAAGTACCATAGCTCA+AGG + chr1.4:17091823-17091842 None:intergenic 50.0%
!! CAAGAAGCATTCTTCCAGCC+AGG - chr1.4:17091850-17091869 MS.gene070697:CDS 50.0%
GACTTTATCACTGCCCTGGC+TGG + chr1.4:17091867-17091886 None:intergenic 55.0%
GGACAATGATGCCATGGCCT+AGG - chr1.4:17091266-17091285 MS.gene070697:CDS 55.0%
GGTATAGGTAGCCTAGGCCA+TGG + chr1.4:17091280-17091299 None:intergenic 55.0%
!! GCTTTTGGTGTGGAGCAAGG+AGG - chr1.4:17091386-17091405 MS.gene070697:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 17091229 17091986 17091229 ID=MS.gene070697
chr1.4 mRNA 17091229 17091986 17091229 ID=MS.gene070697.t1;Parent=MS.gene070697
chr1.4 exon 17091648 17091986 17091648 ID=MS.gene070697.t1.exon1;Parent=MS.gene070697.t1
chr1.4 CDS 17091648 17091986 17091648 ID=cds.MS.gene070697.t1;Parent=MS.gene070697.t1
chr1.4 exon 17091229 17091408 17091229 ID=MS.gene070697.t1.exon2;Parent=MS.gene070697.t1
chr1.4 CDS 17091229 17091408 17091229 ID=cds.MS.gene070697.t1;Parent=MS.gene070697.t1
Gene Sequence

>MS.gene070697

ATGCATCCTACTACACTTCCCAAGGGTATGGCCAAATGGACAATGATGCCATGGCCTAGGCTACCTATACCATTATCAATTGACAAGTTTCCAACTCACAATGTAAGCTTGTGTTTTGACTTGAAAGAATTAGATTTGAGCAATCAAAGAGGTATAAGCTTTTGGTGTGGAGCAAGGAGGCAAATTAGATACCAAGATGAGGATGAAGATAGAGATGATGAGTATGGACACAATGAGGAAATTTCAAAGTTGGAATTCTATAGTCAATCAGCAAGAGGAGAAGCACTCATTGTTCATGCACTTGTGGACCAAATTGAAGTAGAAGTACTTATCTTCAAGGTAAGTTTGGAATTTATGTCAATAATTGCTTTTAAGAGTCTTGCTATCCAATAACCAATAAAACAAACAAATTATTTAAAAATATATGAATCTACTTCTTTTTTTTCTAATTAGAAAAAGGTAAGAAGCACATTAAAATTCTTGTGATTTTACCAAATTTTTGTTTTGCAAATGGTATGCTGATTACTCTAAAAATATATTTCTTGATAGAAATTTAGTGACAAACCATTTCATTTCAGGGGTTTTCTTCATCCTTGAGCTATGGTACTTCCCCTGACCCAACAAGAAGCATTCTTCCAGCCAGGGCAGTGATAAAGTCTATAGATAGAATAAAAGGGCCTTTTGACCCTGCCAATATTGAGTATCTGCAGAAGGATGTTCCATGGGAAGAATTCAAGACAAATATTCTTTCTAGCTAA

Protein sequence

>MS.gene070697.t1

MHPTTLPKGMAKWTMMPWPRLPIPLSIDKFPTHNVSLCFDLKELDLSNQRGISFWCGARRQIRYQDEDEDRDDEYGHNEEISKLEFYSQSARGEALIVHALVDQIEVEVLIFKGFSSSLSYGTSPDPTRSILPARAVIKSIDRIKGPFDPANIEYLQKDVPWEEFKTNILSS