Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072632.t1 | XP_003626086.1 | 98.3 | 232 | 3 | 1 | 1 | 231 | 58 | 289 | 3.50E-114 | 421 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072632.t1 | G7KVG1 | 98.3 | 232 | 3 | 1 | 1 | 231 | 58 | 289 | 2.6e-114 | 421.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene072632 | 0.819631 | 1.05E-52 | -1.69E-46 |
| MS.gene049983 | MS.gene072632 | 0.801159 | 1.03E-48 | -1.69E-46 |
| MS.gene050442 | MS.gene072632 | 0.804517 | 2.08E-49 | -1.69E-46 |
| MS.gene050654 | MS.gene072632 | 0.804344 | 2.26E-49 | -1.69E-46 |
| MS.gene050737 | MS.gene072632 | 0.802945 | 4.41E-49 | -1.69E-46 |
| MS.gene050762 | MS.gene072632 | 0.837275 | 5.72E-57 | -1.69E-46 |
| MS.gene050763 | MS.gene072632 | 0.835734 | 1.41E-56 | -1.69E-46 |
| MS.gene050826 | MS.gene072632 | 0.957288 | 3.90E-115 | -1.69E-46 |
| MS.gene050941 | MS.gene072632 | 0.814523 | 1.48E-51 | -1.69E-46 |
| MS.gene051198 | MS.gene072632 | 0.807347 | 5.28E-50 | -1.69E-46 |
| MS.gene051490 | MS.gene072632 | 0.811955 | 5.40E-51 | -1.69E-46 |
| MS.gene051800 | MS.gene072632 | 0.822672 | 2.09E-53 | -1.69E-46 |
| MS.gene051856 | MS.gene072632 | 0.802692 | 4.97E-49 | -1.69E-46 |
| MS.gene052463 | MS.gene072632 | 0.807471 | 4.97E-50 | -1.69E-46 |
| MS.gene052674 | MS.gene072632 | 0.83028 | 3.21E-55 | -1.69E-46 |
| MS.gene052675 | MS.gene072632 | 0.828753 | 7.54E-55 | -1.69E-46 |
| MS.gene052677 | MS.gene072632 | 0.833608 | 4.84E-56 | -1.69E-46 |
| MS.gene053166 | MS.gene072632 | 0.82074 | 5.85E-53 | -1.69E-46 |
| MS.gene05350 | MS.gene072632 | 0.811854 | 5.69E-51 | -1.69E-46 |
| MS.gene053811 | MS.gene072632 | 0.808539 | 2.95E-50 | -1.69E-46 |
| MS.gene05415 | MS.gene072632 | 0.801129 | 1.04E-48 | -1.69E-46 |
| MS.gene054236 | MS.gene072632 | 0.807024 | 6.19E-50 | -1.69E-46 |
| MS.gene05423 | MS.gene072632 | 0.803807 | 2.92E-49 | -1.69E-46 |
| MS.gene054379 | MS.gene072632 | 0.845541 | 3.81E-59 | -1.69E-46 |
| MS.gene054468 | MS.gene072632 | 0.815714 | 8.02E-52 | -1.69E-46 |
| MS.gene05468 | MS.gene072632 | 0.824623 | 7.30E-54 | -1.69E-46 |
| MS.gene055227 | MS.gene072632 | 0.808512 | 2.99E-50 | -1.69E-46 |
| MS.gene055310 | MS.gene072632 | 0.806692 | 7.27E-50 | -1.69E-46 |
| MS.gene055701 | MS.gene072632 | 0.815079 | 1.11E-51 | -1.69E-46 |
| MS.gene055926 | MS.gene072632 | 0.872399 | 3.28E-67 | -1.69E-46 |
| MS.gene05604 | MS.gene072632 | 0.801024 | 1.09E-48 | -1.69E-46 |
| MS.gene056480 | MS.gene072632 | 0.819278 | 1.26E-52 | -1.69E-46 |
| MS.gene05761 | MS.gene072632 | 0.812602 | 3.90E-51 | -1.69E-46 |
| MS.gene058010 | MS.gene072632 | 0.808882 | 2.49E-50 | -1.69E-46 |
| MS.gene058081 | MS.gene072632 | 0.825367 | 4.87E-54 | -1.69E-46 |
| MS.gene058647 | MS.gene072632 | 0.807757 | 4.32E-50 | -1.69E-46 |
| MS.gene059128 | MS.gene072632 | 0.800958 | 1.13E-48 | -1.69E-46 |
| MS.gene059161 | MS.gene072632 | 0.831014 | 2.12E-55 | -1.69E-46 |
| MS.gene059283 | MS.gene072632 | 0.80981 | 1.57E-50 | -1.69E-46 |
| MS.gene059438 | MS.gene072632 | 0.804713 | 1.89E-49 | -1.69E-46 |
| MS.gene059439 | MS.gene072632 | 0.823887 | 1.09E-53 | -1.69E-46 |
| MS.gene059664 | MS.gene072632 | 0.805495 | 1.30E-49 | -1.69E-46 |
| MS.gene059952 | MS.gene072632 | 0.830102 | 3.55E-55 | -1.69E-46 |
| MS.gene060122 | MS.gene072632 | -0.804729 | 1.88E-49 | -1.69E-46 |
| MS.gene060322 | MS.gene072632 | 0.837435 | 5.21E-57 | -1.69E-46 |
| MS.gene060373 | MS.gene072632 | 0.815608 | 8.47E-52 | -1.69E-46 |
| MS.gene06052 | MS.gene072632 | 0.837765 | 4.28E-57 | -1.69E-46 |
| MS.gene060802 | MS.gene072632 | 0.849687 | 2.76E-60 | -1.69E-46 |
| MS.gene060803 | MS.gene072632 | 0.808713 | 2.71E-50 | -1.69E-46 |
| MS.gene060850 | MS.gene072632 | 0.800418 | 1.45E-48 | -1.69E-46 |
| MS.gene060851 | MS.gene072632 | 0.811404 | 7.12E-51 | -1.69E-46 |
| MS.gene060852 | MS.gene072632 | 0.811092 | 8.32E-51 | -1.69E-46 |
| MS.gene060932 | MS.gene072632 | 0.812331 | 4.47E-51 | -1.69E-46 |
| MS.gene060998 | MS.gene072632 | 0.814261 | 1.69E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072632.t1 | MTR_7g110880 | 98.276 | 232 | 3 | 1 | 1 | 231 | 58 | 289 | 1.35e-167 | 463 |
| MS.gene072632.t1 | MTR_1g116590 | 47.887 | 71 | 35 | 2 | 156 | 224 | 41 | 111 | 1.12e-13 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072632.t1 | AT3G62070 | 46.414 | 237 | 76 | 11 | 2 | 231 | 36 | 228 | 1.03e-40 | 139 |
| MS.gene072632.t1 | AT4G01670 | 46.983 | 232 | 83 | 7 | 16 | 229 | 34 | 243 | 2.34e-40 | 139 |
| MS.gene072632.t1 | AT2G46940 | 43.860 | 228 | 91 | 7 | 3 | 228 | 60 | 252 | 2.64e-40 | 139 |
| MS.gene072632.t1 | AT2G31560 | 41.489 | 94 | 28 | 2 | 156 | 222 | 102 | 195 | 9.40e-14 | 68.2 |
| MS.gene072632.t1 | AT2G31560 | 41.489 | 94 | 28 | 2 | 156 | 222 | 102 | 195 | 9.40e-14 | 68.2 |
| MS.gene072632.t1 | AT2G31560 | 41.489 | 94 | 28 | 2 | 156 | 222 | 102 | 195 | 9.40e-14 | 68.2 |
| MS.gene072632.t1 | AT2G43340 | 41.489 | 94 | 28 | 2 | 156 | 222 | 89 | 182 | 5.25e-13 | 65.9 |
| MS.gene072632.t1 | AT1G05870 | 41.237 | 97 | 29 | 3 | 156 | 224 | 88 | 184 | 1.00e-12 | 65.1 |
| MS.gene072632.t1 | AT1G05870 | 41.237 | 97 | 29 | 3 | 156 | 224 | 88 | 184 | 1.00e-12 | 65.1 |
| MS.gene072632.t1 | AT1G05870 | 41.237 | 97 | 29 | 3 | 156 | 224 | 88 | 184 | 1.00e-12 | 65.1 |
| MS.gene072632.t1 | AT1G05870 | 41.237 | 97 | 29 | 3 | 156 | 224 | 88 | 184 | 1.00e-12 | 65.1 |
| MS.gene072632.t1 | AT1G05870 | 41.237 | 97 | 29 | 3 | 156 | 224 | 88 | 184 | 1.00e-12 | 65.1 |
| MS.gene072632.t1 | AT1G05870 | 41.237 | 97 | 29 | 3 | 156 | 224 | 88 | 184 | 1.00e-12 | 65.1 |
| MS.gene072632.t1 | AT1G05870 | 41.237 | 97 | 29 | 3 | 156 | 224 | 88 | 184 | 1.00e-12 | 65.1 |
| MS.gene072632.t1 | AT1G05870 | 41.237 | 97 | 29 | 3 | 156 | 224 | 88 | 184 | 1.00e-12 | 65.1 |
| MS.gene072632.t1 | AT1G05870 | 41.237 | 97 | 29 | 3 | 156 | 224 | 88 | 184 | 1.00e-12 | 65.1 |
| MS.gene072632.t1 | AT1G05870 | 41.237 | 97 | 29 | 3 | 156 | 224 | 88 | 184 | 1.00e-12 | 65.1 |
| MS.gene072632.t1 | AT1G05870 | 41.237 | 97 | 29 | 3 | 156 | 224 | 88 | 184 | 1.00e-12 | 65.1 |
| MS.gene072632.t1 | AT1G05870 | 41.237 | 97 | 29 | 3 | 156 | 224 | 88 | 184 | 1.00e-12 | 65.1 |
Find 0 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTGTTTTATATTATGT+AGG | - | 50581:36878-36897 | MS.gene072632:CDS | 10.0% |
| !! | ATAAACATTATAAGATTGTT+TGG | - | 50581:36747-36766 | MS.gene072632:CDS | 15.0% |
| !! | AAAACAATTTATGACATGTT+CGG | - | 50581:36781-36800 | MS.gene072632:CDS | 20.0% |
| ! | AAATAGTTGGGAGAATTTAT+GGG | - | 50581:36389-36408 | MS.gene072632:CDS | 25.0% |
| AACCAAAATTGAGAATTACC+GGG | + | 50581:36684-36703 | None:intergenic | 30.0% | |
| AGAAAAGAAACAAAGGAAAG+TGG | - | 50581:36309-36328 | MS.gene072632:intron | 30.0% | |
| ATCAGCTTCAAAACTTTCAA+TGG | + | 50581:36097-36116 | None:intergenic | 30.0% | |
| GAAATAGTTGGGAGAATTTA+TGG | - | 50581:36388-36407 | MS.gene072632:CDS | 30.0% | |
| GAACCAAAATTGAGAATTAC+CGG | + | 50581:36685-36704 | None:intergenic | 30.0% | |
| GAGAAAGAGAAATTAGAGAA+TGG | - | 50581:36435-36454 | MS.gene072632:CDS | 30.0% | |
| GTAATTCACCTATTGCTAAT+TGG | - | 50581:36646-36665 | MS.gene072632:CDS | 30.0% | |
| TAAGCGCGTATCATATATTA+TGG | + | 50581:36825-36844 | None:intergenic | 30.0% | |
| TGAAGCAAGAAAAGAAACAA+AGG | - | 50581:36302-36321 | MS.gene072632:intron | 30.0% | |
| ! | AATGAGAAGTGTGTTTGAAA+GGG | - | 50581:36365-36384 | MS.gene072632:intron | 30.0% |
| ! | TTTGGTTCATATTTCCGATT+AGG | - | 50581:36697-36716 | MS.gene072632:CDS | 30.0% |
| !!! | ATTGAAAGTTTTGAAGCTGA+TGG | - | 50581:36096-36115 | MS.gene072632:CDS | 30.0% |
| AGAAACAAAGGAAAGTGGTT+TGG | - | 50581:36314-36333 | MS.gene072632:intron | 35.0% | |
| ATGATCAGTTACTACAACCA+GGG | - | 50581:36160-36179 | MS.gene072632:intron | 35.0% | |
| GAAACAAAGGAAAGTGGTTT+GGG | - | 50581:36315-36334 | MS.gene072632:intron | 35.0% | |
| GTTAAAGCTTGTAGAGATCT+TGG | - | 50581:36552-36571 | MS.gene072632:CDS | 35.0% | |
| GTTTGAAAGGGGAAATAGTT+GGG | - | 50581:36377-36396 | MS.gene072632:CDS | 35.0% | |
| TAGAGATCTTGGATTTGAGT+TGG | - | 50581:36563-36582 | MS.gene072632:CDS | 35.0% | |
| TAGGAGTTTGTGTATGGATT+TGG | - | 50581:36524-36543 | MS.gene072632:CDS | 35.0% | |
| TCAACACTTGATACTAGTAG+TGG | - | 50581:36621-36640 | MS.gene072632:CDS | 35.0% | |
| TGTTTGAAAGGGGAAATAGT+TGG | - | 50581:36376-36395 | MS.gene072632:CDS | 35.0% | |
| TTAGAGAATGGTGTGAATAG+TGG | - | 50581:36447-36466 | MS.gene072632:CDS | 35.0% | |
| ! | AGGTAGTTGTTATTCGTTAC+CGG | - | 50581:36209-36228 | MS.gene072632:intron | 35.0% |
| ! | ATGAGAAGTGTGTTTGAAAG+GGG | - | 50581:36366-36385 | MS.gene072632:intron | 35.0% |
| ! | CTCGAATAAGTTGACCTAAT+CGG | + | 50581:36714-36733 | None:intergenic | 35.0% |
| ! | GAATGAGAAGTGTGTTTGAA+AGG | - | 50581:36364-36383 | MS.gene072632:intron | 35.0% |
| AAACTCCTATTCCCACCTTT+AGG | + | 50581:36513-36532 | None:intergenic | 40.0% | |
| AAATTGAGAATTACCGGGAC+CGG | + | 50581:36679-36698 | None:intergenic | 40.0% | |
| ACACTTGATACTAGTAGTGG+TGG | - | 50581:36624-36643 | MS.gene072632:CDS | 40.0% | |
| ATAGCATCTGCCACCAAATA+CGG | - | 50581:36956-36975 | MS.gene072632:CDS | 40.0% | |
| ATGGTGATTACTAGGCCTAA+AGG | - | 50581:36495-36514 | MS.gene072632:CDS | 40.0% | |
| ATTGCTAATTGGCGAATCTC+CGG | - | 50581:36657-36676 | MS.gene072632:CDS | 40.0% | |
| GAGATTCGCCAATTAGCAAT+AGG | + | 50581:36657-36676 | None:intergenic | 40.0% | |
| GATGATCAGTTACTACAACC+AGG | - | 50581:36159-36178 | MS.gene072632:intron | 40.0% | |
| TGATGATCCAAGAGATGTGA+AGG | - | 50581:36901-36920 | MS.gene072632:CDS | 40.0% | |
| TGATTACTAGGCCTAAAGGT+GGG | - | 50581:36499-36518 | MS.gene072632:CDS | 40.0% | |
| TGGGAATAGGAGTTTGTGTA+TGG | - | 50581:36518-36537 | MS.gene072632:CDS | 40.0% | |
| ! | GATGGTGAATTTTCTGCTGA+TGG | - | 50581:36132-36151 | MS.gene072632:CDS | 40.0% |
| ! | GTCCCGGTAATTCTCAATTT+TGG | - | 50581:36679-36698 | MS.gene072632:CDS | 40.0% |
| ! | TGATTCATTTTCAGAGCCAG+AGG | + | 50581:36190-36209 | None:intergenic | 40.0% |
| !! | TCTGGCTCTGAAAATGAATC+AGG | - | 50581:36189-36208 | MS.gene072632:intron | 40.0% |
| !!! | GAAAGTTTTGAAGCTGATGG+TGG | - | 50581:36099-36118 | MS.gene072632:CDS | 40.0% |
| GTGATTACTAGGCCTAAAGG+TGG | - | 50581:36498-36517 | MS.gene072632:CDS | 45.0% | |
| TTCACTCCAACAACACTCAC+TGG | + | 50581:36264-36283 | None:intergenic | 45.0% | |
| ! | ACAAAGGAAAGTGGTTTGGG+AGG | - | 50581:36318-36337 | MS.gene072632:intron | 45.0% |
| ! | GAGAGAGTGATCAGAGTACT+GGG | - | 50581:36469-36488 | MS.gene072632:CDS | 45.0% |
| ! | TTGGTTCCAGTGAGTGTTGT+TGG | - | 50581:36255-36274 | MS.gene072632:intron | 45.0% |
| !! | AGAGAGTGATCAGAGTACTG+GGG | - | 50581:36470-36489 | MS.gene072632:CDS | 45.0% |
| AGTCGTGCCTTCACATCTCT+TGG | + | 50581:36911-36930 | None:intergenic | 50.0% | |
| CTAGGCCTAAAGGTGGGAAT+AGG | - | 50581:36505-36524 | MS.gene072632:CDS | 50.0% | |
| GGAGAGAGTGATCAGAGTAC+TGG | - | 50581:36468-36487 | MS.gene072632:CDS | 50.0% | |
| GTAGGAGAAGAAGGAGGAGA+AGG | - | 50581:36340-36359 | MS.gene072632:intron | 50.0% | |
| GTTTGGGAGGTAGGAGAAGA+AGG | - | 50581:36331-36350 | MS.gene072632:intron | 50.0% | |
| ! | AGGAAAGTGGTTTGGGAGGT+AGG | - | 50581:36322-36341 | MS.gene072632:intron | 50.0% |
| ! | CTGGGGTGATGGTGATTACT+AGG | - | 50581:36487-36506 | MS.gene072632:CDS | 50.0% |
| ! | GAAGGCACGACTCAAAGTGT+GGG | - | 50581:36919-36938 | MS.gene072632:CDS | 50.0% |
| ! | GATGGTGGTGATGAAGCAGA+TGG | - | 50581:36114-36133 | MS.gene072632:CDS | 50.0% |
| ! | TGAAGGCACGACTCAAAGTG+TGG | - | 50581:36918-36937 | MS.gene072632:CDS | 50.0% |
| !! | TGATCAGAGTACTGGGGTGA+TGG | - | 50581:36476-36495 | MS.gene072632:CDS | 50.0% |
| AATTGGCGAATCTCCGGTCC+CGG | - | 50581:36663-36682 | MS.gene072632:CDS | 55.0% | |
| ACCAAACTCCTCCGTCTTGG+TGG | + | 50581:36240-36259 | None:intergenic | 55.0% | |
| ACCACCAAGACGGAGGAGTT+TGG | - | 50581:36236-36255 | MS.gene072632:intron | 55.0% | |
| ACGACTCAAAGTGTGGGCAC+AGG | - | 50581:36925-36944 | MS.gene072632:CDS | 55.0% | |
| CTACAACCAGGGTTGTCCTC+TGG | - | 50581:36171-36190 | MS.gene072632:intron | 55.0% | |
| GGAACCAAACTCCTCCGTCT+TGG | + | 50581:36243-36262 | None:intergenic | 55.0% | |
| TACCGGTGACACCACCAAGA+CGG | - | 50581:36226-36245 | MS.gene072632:intron | 55.0% | |
| TGGGAGGTAGGAGAAGAAGG+AGG | - | 50581:36334-36353 | MS.gene072632:intron | 55.0% | |
| TCAGAGCCAGAGGACAACCC+TGG | + | 50581:36180-36199 | None:intergenic | 60.0% | |
| !! | CTCCGTCTTGGTGGTGTCAC+CGG | + | 50581:36231-36250 | None:intergenic | 60.0% |
| CGGTGACACCACCAAGACGG+AGG | - | 50581:36229-36248 | MS.gene072632:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 50581 | gene | 36075 | 36985 | 36075 | ID=MS.gene072632 |
| 50581 | mRNA | 36075 | 36985 | 36075 | ID=MS.gene072632.t1;Parent=MS.gene072632 |
| 50581 | exon | 36376 | 36985 | 36376 | ID=MS.gene072632.t1.exon1;Parent=MS.gene072632.t1 |
| 50581 | CDS | 36376 | 36985 | 36376 | ID=cds.MS.gene072632.t1;Parent=MS.gene072632.t1 |
| 50581 | exon | 36075 | 36160 | 36075 | ID=MS.gene072632.t1.exon2;Parent=MS.gene072632.t1 |
| 50581 | CDS | 36075 | 36160 | 36075 | ID=cds.MS.gene072632.t1;Parent=MS.gene072632.t1 |
| Gene Sequence |
| Protein sequence |
