AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07450


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07450.t1 MTR_1g077030 100.000 160 0 0 1 160 1 160 3.65e-117 332
MS.gene07450.t1 MTR_6g064960 79.012 162 32 2 1 160 1 162 1.77e-91 267
MS.gene07450.t1 MTR_6g036660 56.471 85 7 1 76 160 9 63 3.70e-21 84.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07450.t1 AT4G15093 66.875 160 51 2 3 160 4 163 2.27e-77 231

Find 0 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGATAGATATAAAACAAAAA+TGG + 8694:1098-1117 MS.gene07450:intergenic 15.0%
!! CAAGATTTATTCCATTAAAT+GGG + 8694:737-756 MS.gene07450:intergenic 20.0%
! AAGAAATTGTAGCAATAGAT+TGG - 8694:804-823 MS.gene07450:intron 25.0%
! ATATGCATACATTACACATT+AGG - 8694:859-878 MS.gene07450:intron 25.0%
! ATTACATCTTGAAATTTGAG+CGG - 8694:663-682 MS.gene07450:intron 25.0%
! ATTGTGATTAACAACGTTAA+CGG + 8694:519-538 MS.gene07450:intergenic 25.0%
! CCAAGATTTATTCCATTAAA+TGG + 8694:738-757 MS.gene07450:intergenic 25.0%
! CCATTTAATGGAATAAATCT+TGG - 8694:735-754 MS.gene07450:intron 25.0%
! GGAATCTGAATTAAACAATT+AGG + 8694:942-961 MS.gene07450:intergenic 25.0%
! TTTATTCCATTAAATGGGAT+CGG + 8694:732-751 MS.gene07450:intergenic 25.0%
!!! TTGCTTTTCCTAAGTTATAA+TGG + 8694:1347-1366 MS.gene07450:intergenic 25.0%
AAAACAAAAATGGACCATTG+TGG + 8694:1088-1107 MS.gene07450:intergenic 30.0%
AATGAAACCATCCATGATTT+CGG - 8694:536-555 MS.gene07450:CDS 30.0%
CTATTGAATCATAACTGATC+CGG - 8694:902-921 MS.gene07450:intron 30.0%
GATTGGATAAACAAACATTG+AGG - 8694:821-840 MS.gene07450:intron 30.0%
GGTACTTACCATTATAACTT+AGG - 8694:1336-1355 MS.gene07450:CDS 30.0%
TAGCTCTTCAATTGATACTT+AGG + 8694:1035-1054 MS.gene07450:intergenic 30.0%
TATACCAGTATGTCAACTTT+CGG - 8694:1296-1315 MS.gene07450:CDS 30.0%
TATTGAATCATAACTGATCC+GGG - 8694:903-922 MS.gene07450:intron 30.0%
TCGGTTTCATCAAATAGAAA+TGG - 8694:1315-1334 MS.gene07450:CDS 30.0%
TTAATTCAGATTCCGAACTT+TGG - 8694:948-967 MS.gene07450:intron 30.0%
TTTCGATTCTCAAACAGATT+TGG + 8694:780-799 MS.gene07450:intergenic 30.0%
! GAAACGTTTTACATATCACA+TGG - 8694:365-384 MS.gene07450:CDS 30.0%
!!! TTTCGGTATCGATTTGTTTT+TGG - 8694:995-1014 MS.gene07450:intron 30.0%
AATAGCTCCTCTTAAAGATG+AGG - 8694:1365-1384 MS.gene07450:CDS 35.0%
ATAGCTCCTCTTAAAGATGA+GGG - 8694:1366-1385 MS.gene07450:CDS 35.0%
ATGACCAGAGATAACAAGAA+TGG + 8694:477-496 MS.gene07450:intergenic 35.0%
ATTCTTGACATCATGGAAAG+AGG - 8694:430-449 MS.gene07450:CDS 35.0%
CATGGAAATTCTTGACATCA+TGG - 8694:423-442 MS.gene07450:CDS 35.0%
GAAACTAGAGTCCAATCATT+CGG + 8694:979-998 MS.gene07450:intergenic 35.0%
GATACTTGAGCTGCAAATAA+AGG + 8694:1134-1153 MS.gene07450:intergenic 35.0%
GCATACATTACACATTAGGT+TGG - 8694:863-882 MS.gene07450:intron 35.0%
TAGATGAAACAATTCCTGCA+TGG - 8694:405-424 MS.gene07450:CDS 35.0%
TGATTAACAACGTTAACGGT+TGG + 8694:515-534 MS.gene07450:intergenic 35.0%
TGCAGGAATTGTTTCATCTA+TGG + 8694:405-424 MS.gene07450:intergenic 35.0%
TGGACGAAGATAAAAAACGT+GGG - 8694:1223-1242 MS.gene07450:CDS 35.0%
! GAATGATTGGACTCTAGTTT+CGG - 8694:978-997 MS.gene07450:intron 35.0%
! TCTTGTTATCTCTGGTCATT+GGG - 8694:478-497 MS.gene07450:CDS 35.0%
! TTCTTGTTATCTCTGGTCAT+TGG - 8694:477-496 MS.gene07450:CDS 35.0%
AAAAAACGTGGGCTTGATCA+TGG - 8694:1234-1253 MS.gene07450:CDS 40.0%
AAAACCACCGAAATCATGGA+TGG + 8694:546-565 MS.gene07450:intergenic 40.0%
AAAGTTAGCAAAGAGGGTGA+AGG - 8694:1170-1189 MS.gene07450:CDS 40.0%
AATAAATCTTGGTTGTGCGC+TGG - 8694:746-765 MS.gene07450:intron 40.0%
ACTCATGTTGATGTATCCAG+AGG - 8694:1269-1288 MS.gene07450:CDS 40.0%
GAAACCGAAAGTTGACATAC+TGG + 8694:1303-1322 MS.gene07450:intergenic 40.0%
GAGATAACAAGAATGGCAGA+TGG + 8694:470-489 MS.gene07450:intergenic 40.0%
GATGTCAAGAATTTCCATGC+AGG + 8694:422-441 MS.gene07450:intergenic 40.0%
GTGGACGAAGATAAAAAACG+TGG - 8694:1222-1241 MS.gene07450:CDS 40.0%
GTTTCATCTATGGCTAGTGT+TGG + 8694:395-414 MS.gene07450:intergenic 40.0%
TAGACACCCTCATCTTTAAG+AGG + 8694:1375-1394 MS.gene07450:intergenic 40.0%
TAGGAAAACCACCGAAATCA+TGG + 8694:550-569 MS.gene07450:intergenic 40.0%
TCTGGATACATCAACATGAG+TGG + 8694:1270-1289 MS.gene07450:intergenic 40.0%
TGAGCTGCAAATAAAGGAAC+AGG + 8694:1128-1147 MS.gene07450:intergenic 40.0%
TTCACCCTCTTTGCTAACTT+TGG + 8694:1171-1190 MS.gene07450:intergenic 40.0%
! ATGATTTCGGTGGTTTTCCT+AGG - 8694:549-568 MS.gene07450:CDS 40.0%
! GTTTTCCAATCTCTCCACAA+TGG - 8694:1071-1090 MS.gene07450:intron 40.0%
! TCTGCCATTCTTGTTATCTC+TGG - 8694:470-489 MS.gene07450:CDS 40.0%
!! TTTTCCTAGGAGCATGTACA+AGG - 8694:562-581 MS.gene07450:CDS 40.0%
ACTAGAGTCCAATCATTCGG+TGG + 8694:976-995 MS.gene07450:intergenic 45.0%
ATGGACCATTGTGGAGAGAT+TGG + 8694:1079-1098 MS.gene07450:intergenic 45.0%
CAAACCTTGTACATGCTCCT+AGG + 8694:569-588 MS.gene07450:intergenic 45.0%
CATACTGGTATATCAGCCTC+TGG + 8694:1288-1307 MS.gene07450:intergenic 45.0%
GAAACCATCCATGATTTCGG+TGG - 8694:539-558 MS.gene07450:CDS 45.0%
GGCTCCAAAGTTAGCAAAGA+GGG - 8694:1164-1183 MS.gene07450:intron 45.0%
GTGTAGCCGATCCCATTTAA+TGG - 8694:723-742 MS.gene07450:intron 45.0%
TGGGCTTGATCATGGAACAT+GGG - 8694:1242-1261 MS.gene07450:CDS 45.0%
TTAGGCCATCTTTGCTTACC+CGG + 8694:924-943 MS.gene07450:intergenic 45.0%
! AAGGAACTGATCGAAGCATC+TGG - 8694:1189-1208 MS.gene07450:CDS 45.0%
! AGGAACTGATCGAAGCATCT+GGG - 8694:1190-1209 MS.gene07450:CDS 45.0%
! CATTCGGTGGAACCAAAGTT+CGG + 8694:963-982 MS.gene07450:intergenic 45.0%
! CTTTGGTTCCACCGAATGAT+TGG - 8694:965-984 MS.gene07450:intron 45.0%
AGAATGGCAGATGGTCTCTC+AGG + 8694:461-480 MS.gene07450:intergenic 50.0%
AGCTCAAGTATCCAGCACCA+GGG - 8694:1142-1161 MS.gene07450:intron 50.0%
CTGATCCGGGTAAGCAAAGA+TGG - 8694:916-935 MS.gene07450:intron 50.0%
GGGCTCCAAAGTTAGCAAAG+AGG - 8694:1163-1182 MS.gene07450:intron 50.0%
GTGGGCTTGATCATGGAACA+TGG - 8694:1241-1260 MS.gene07450:CDS 50.0%
!! TTTGCTAACTTTGGAGCCCC+TGG + 8694:1162-1181 MS.gene07450:intergenic 50.0%
CAGCTCAAGTATCCAGCACC+AGG - 8694:1141-1160 MS.gene07450:intron 55.0%
GCTCAAGTATCCAGCACCAG+GGG - 8694:1143-1162 MS.gene07450:intron 55.0%
! AGCATCTGGGTTGAGTCGAG+TGG - 8694:1203-1222 MS.gene07450:CDS 55.0%
!! AACTTTGGAGCCCCTGGTGC+TGG + 8694:1156-1175 MS.gene07450:intergenic 60.0%


Chromosome Type Strat End Strand Name
8694 gene 353 1395 353 ID=MS.gene07450
8694 mRNA 353 1395 353 ID=MS.gene07450.t1;Parent=MS.gene07450
8694 exon 1165 1395 1165 ID=MS.gene07450.t1.exon1;Parent=MS.gene07450.t1
8694 CDS 1165 1395 1165 ID=cds.MS.gene07450.t1;Parent=MS.gene07450.t1
8694 exon 353 604 353 ID=MS.gene07450.t1.exon2;Parent=MS.gene07450.t1
8694 CDS 353 604 353 ID=cds.MS.gene07450.t1;Parent=MS.gene07450.t1
Gene Sequence

>MS.gene07450

ATGGCATTGAAGGAAACGTTTTACATATCACATGGATCACCAACACTAGCCATAGATGAAACAATTCCTGCATGGAAATTCTTGACATCATGGAAAGAGGTGTTTCCTGAGAGACCATCTGCCATTCTTGTTATCTCTGGTCATTGGGACACTTCTGTTCCAACCGTTAACGTTGTTAATCACAATGAAACCATCCATGATTTCGGTGGTTTTCCTAGGAGCATGTACAAGGTTTGTTTCTATGTACTTTTGTAACATGTCTCATGTCAATGATTTCAATTATATTTAGACTTCATGATCACAATTTTAAATTACATCTTGAAATTTGAGCGGATAATTTATGATAGAACATTGTGATGTCGTTTGATCTGTGTAGCCGATCCCATTTAATGGAATAAATCTTGGTTGTGCGCTGGCAGAGAATCCAAATCTGTTTGAGAATCGAAAAATTAAGAAATTGTAGCAATAGATTGGATAAACAAACATTGAGGCTGTAAATTGTAATAATATGCATACATTACACATTAGGTTGGTTGAATTTCAAATTTTCTATTGAATCATAACTGATCCGGGTAAGCAAAGATGGCCTAATTGTTTAATTCAGATTCCGAACTTTGGTTCCACCGAATGATTGGACTCTAGTTTCGGTATCGATTTGTTTTTGGTTACTTAAGCTGATCCTAAGTATCAATTGAAGAGCTAATAATTCTAATTCGACGTTTTCCAATCTCTCCACAATGGTCCATTTTTGTTTTATATCTATCTGTTTAAACCTGTTCCTTTATTTGCAGCTCAAGTATCCAGCACCAGGGGCTCCAAAGTTAGCAAAGAGGGTGAAGGAACTGATCGAAGCATCTGGGTTGAGTCGAGTGGACGAAGATAAAAAACGTGGGCTTGATCATGGAACATGGGTGCCACTCATGTTGATGTATCCAGAGGCTGATATACCAGTATGTCAACTTTCGGTTTCATCAAATAGAAATGGTACTTACCATTATAACTTAGGAAAAGCAATAGCTCCTCTTAAAGATGAGGGTGTCTAA

Protein sequence

>MS.gene07450.t1

MALKETFYISHGSPTLAIDETIPAWKFLTSWKEVFPERPSAILVISGHWDTSVPTVNVVNHNETIHDFGGFPRSMYKLKYPAPGAPKLAKRVKELIEASGLSRVDEDKKRGLDHGTWVPLMLMYPEADIPVCQLSVSSNRNGTYHYNLGKAIAPLKDEGV