AlfalfaGEDB | MS.gene08545

M. sativa cultivar XinJiangDaYe / MS.gene08545

KEGG: -
GO: GO:0003674 GO:0003824 GO:0004045 GO:0016787 GO:0016788 GO:0052689 GO:0140098 GO:0140101
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08545.t1	RHN42614.1	97.8	89	2	0	1	89	130	218	5.30E-42	179.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08545.t1	Q6NLS8	68.6	86	27	0	1	86	131	216	2.7e-26	119.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08545.t1	A0A396GSX3	97.8	89	2	0	1	89	130	218	3.8e-42	179.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene08545	0.801999	6.91E-49	-1.69E-46
MS.gene049341	MS.gene08545	0.812346	4.44E-51	-1.69E-46
MS.gene049382	MS.gene08545	0.802905	4.49E-49	-1.69E-46
MS.gene049756	MS.gene08545	0.828082	1.10E-54	-1.69E-46
MS.gene04984	MS.gene08545	0.810436	1.15E-50	-1.69E-46
MS.gene050277	MS.gene08545	0.852348	4.90E-61	-1.69E-46
MS.gene050303	MS.gene08545	0.82879	7.39E-55	-1.69E-46
MS.gene050317	MS.gene08545	0.802472	5.52E-49	-1.69E-46
MS.gene050438	MS.gene08545	0.812826	3.49E-51	-1.69E-46
MS.gene050719	MS.gene08545	0.832107	1.14E-55	-1.69E-46
MS.gene050721	MS.gene08545	0.837057	6.50E-57	-1.69E-46
MS.gene050724	MS.gene08545	0.825195	5.35E-54	-1.69E-46
MS.gene050815	MS.gene08545	0.820065	8.35E-53	-1.69E-46
MS.gene051063	MS.gene08545	0.823817	1.13E-53	-1.69E-46
MS.gene051245	MS.gene08545	0.844828	5.94E-59	-1.69E-46
MS.gene051349	MS.gene08545	0.812357	4.42E-51	-1.69E-46
MS.gene05134	MS.gene08545	0.808611	2.85E-50	-1.69E-46
MS.gene051633	MS.gene08545	0.830775	2.43E-55	-1.69E-46
MS.gene051746	MS.gene08545	0.804033	2.62E-49	-1.69E-46
MS.gene051748	MS.gene08545	0.802849	4.61E-49	-1.69E-46
MS.gene051757	MS.gene08545	0.806071	9.83E-50	-1.69E-46
MS.gene051903	MS.gene08545	0.80547	1.31E-49	-1.69E-46
MS.gene052286	MS.gene08545	0.844466	7.43E-59	-1.69E-46
MS.gene052302	MS.gene08545	0.846619	1.94E-59	-1.69E-46
MS.gene052309	MS.gene08545	-0.80838	3.19E-50	-1.69E-46
MS.gene052376	MS.gene08545	0.849056	4.13E-60	-1.69E-46
MS.gene052665	MS.gene08545	0.800834	1.20E-48	-1.69E-46
MS.gene052856	MS.gene08545	0.8361	1.14E-56	-1.69E-46
MS.gene052858	MS.gene08545	0.802852	4.61E-49	-1.69E-46
MS.gene052860	MS.gene08545	0.803642	3.16E-49	-1.69E-46
MS.gene053216	MS.gene08545	0.803605	3.22E-49	-1.69E-46
MS.gene053437	MS.gene08545	0.801569	8.46E-49	-1.69E-46
MS.gene055174	MS.gene08545	0.801577	8.43E-49	-1.69E-46
MS.gene055441	MS.gene08545	0.804544	2.05E-49	-1.69E-46
MS.gene056069	MS.gene08545	-0.819376	1.20E-52	-1.69E-46
MS.gene056432	MS.gene08545	0.830815	2.37E-55	-1.69E-46
MS.gene056580	MS.gene08545	0.812032	5.20E-51	-1.69E-46
MS.gene056631	MS.gene08545	0.831864	1.31E-55	-1.69E-46
MS.gene056760	MS.gene08545	0.833262	5.90E-56	-1.69E-46
MS.gene057057	MS.gene08545	0.836737	7.85E-57	-1.69E-46
MS.gene057079	MS.gene08545	0.811175	7.98E-51	-1.69E-46
MS.gene057140	MS.gene08545	0.857148	1.99E-62	-1.69E-46
MS.gene057234	MS.gene08545	0.854515	1.17E-61	-1.69E-46
MS.gene057260	MS.gene08545	0.805492	1.30E-49	-1.69E-46
MS.gene05795	MS.gene08545	0.807745	4.35E-50	-1.69E-46
MS.gene058055	MS.gene08545	0.823088	1.67E-53	-1.69E-46
MS.gene058084	MS.gene08545	0.847245	1.31E-59	-1.69E-46
MS.gene058182	MS.gene08545	0.866283	3.19E-65	-1.69E-46
MS.gene058238	MS.gene08545	0.824037	1.00E-53	-1.69E-46
MS.gene058399	MS.gene08545	0.824932	6.17E-54	-1.69E-46
MS.gene058798	MS.gene08545	0.804772	1.84E-49	-1.69E-46
MS.gene058906	MS.gene08545	0.880782	4.13E-70	-1.69E-46
MS.gene059249	MS.gene08545	0.808109	3.64E-50	-1.69E-46
MS.gene059317	MS.gene08545	0.802895	4.52E-49	-1.69E-46
MS.gene059898	MS.gene08545	0.802437	5.61E-49	-1.69E-46
MS.gene06001	MS.gene08545	0.840504	8.36E-58	-1.69E-46
MS.gene060165	MS.gene08545	0.814461	1.52E-51	-1.69E-46
MS.gene060297	MS.gene08545	0.813717	2.22E-51	-1.69E-46
MS.gene060586	MS.gene08545	0.840584	7.96E-58	-1.69E-46
MS.gene060619	MS.gene08545	0.819481	1.14E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 18 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGAAGCTTCTTCTATTTAA+AGG	0.160898	4.3:-14949593	MS.gene08545:CDS
GCTTCTTCTATTTAAAGGTT+TGG	0.376967	4.3:-14949588	MS.gene08545:CDS
GAATTTCCTCGTCTAAGAAT+TGG	0.379292	4.3:-14950220	MS.gene08545:intron
TTTAAATAGAAGAAGCTTCA+AGG	0.387124	4.3:+14949595	None:intergenic
GTTTGGAAGTGAGTGCAAAA+AGG	0.388013	4.3:-14949571	MS.gene08545:CDS
TGGGAAGCCTCCTGGACAAA+TGG	0.438531	4.3:-14950102	MS.gene08545:CDS
AAGTGCAGACATTACCAGTT+TGG	0.444811	4.3:-14949517	MS.gene08545:CDS
ACAGGGATTGGGAAGCCTCC+TGG	0.466135	4.3:-14950110	MS.gene08545:intron
ATAATCGAATCCTACCAAAC+TGG	0.489126	4.3:+14949503	None:intergenic
GCTTTAGGATCCATTTGTCC+AGG	0.507125	4.3:+14950092	None:intergenic
GCAGACATTACCAGTTTGGT+AGG	0.529698	4.3:-14949513	None:intergenic
TTAGGATCCATTTGTCCAGG+AGG	0.588942	4.3:+14950095	None:intergenic
TTGACGAGGCTTTGCACGAG+GGG	0.595008	4.3:-14949625	MS.gene08545:CDS
GATTGACGAGGCTTTGCACG+AGG	0.603038	4.3:-14949627	MS.gene08545:CDS
TGACGAGGCTTTGCACGAGG+GGG	0.650076	4.3:-14949624	MS.gene08545:CDS
TACTAACCAATTCTTAGACG+AGG	0.656829	4.3:+14950214	None:intergenic
TCTTGTTGGACAGATTGACG+AGG	0.661063	4.3:-14949639	MS.gene08545:intron
ATTGACGAGGCTTTGCACGA+GGG	0.680803	4.3:-14949626	MS.gene08545:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAATATTAATGTGATGAAT+TGG	-	chr4.3:14949913-14949932	MS.gene08545:intron	15.0%
!!!	AAATGAAGATTTTTGTAACT+TGG	+	chr4.3:14950085-14950104	None:intergenic	20.0%
!!!	AGTATTAATCTAAAAAGCAT+GGG	+	chr4.3:14949609-14949628	None:intergenic	20.0%
!	AAAAAAACATGTCAACTGAT+TGG	-	chr4.3:14949874-14949893	MS.gene08545:intron	25.0%
!	AAAAAACATGTCAACTGATT+GGG	-	chr4.3:14949875-14949894	MS.gene08545:intron	25.0%
!	ACTAAGGTTTCAAATTCAAT+TGG	-	chr4.3:14949792-14949811	MS.gene08545:intron	25.0%
!	CAGTATTAATCTAAAAAGCA+TGG	+	chr4.3:14949610-14949629	None:intergenic	25.0%
!	CTAAGGTTTCAAATTCAATT+GGG	-	chr4.3:14949793-14949812	MS.gene08545:intron	25.0%
!	TTGAAGCTTCTTCTATTTAA+AGG	-	chr4.3:14950178-14950197	MS.gene08545:intron	25.0%
!	TTTAAATAGAAGAAGCTTCA+AGG	+	chr4.3:14950179-14950198	None:intergenic	25.0%
!!	GTATTAATCTAAAAAGCATG+GGG	+	chr4.3:14949608-14949627	None:intergenic	25.0%
!!	TTTTCTCTTATCTTTCTTGT+TGG	-	chr4.3:14950118-14950137	MS.gene08545:intron	25.0%
!!!	TTCAATTTGTTTTGTTGACA+GGG	-	chr4.3:14949644-14949663	MS.gene08545:intron	25.0%
!!!	TTTCAATTTGTTTTGTTGAC+AGG	-	chr4.3:14949643-14949662	MS.gene08545:intron	25.0%
!!!	TTTTGAAGCAAAAATGCTTT+AGG	+	chr4.3:14949697-14949716	None:intergenic	25.0%
	AATGAAATAGAATCAGCTAG+TGG	+	chr4.3:14950025-14950044	None:intergenic	30.0%
	AGTTACACTAGAGTAACAAA+AGG	+	chr4.3:14949775-14949794	None:intergenic	30.0%
	GCTTCTTCTATTTAAAGGTT+TGG	-	chr4.3:14950183-14950202	MS.gene08545:intron	30.0%
	TGATGAATTGGAAACCATTT+GGG	-	chr4.3:14949925-14949944	MS.gene08545:intron	30.0%
	TGTTCGAGAAAATTTCATGT+TGG	+	chr4.3:14949980-14949999	None:intergenic	30.0%
	TTGTTACTCTAGTGTAACTA+AGG	-	chr4.3:14949776-14949795	MS.gene08545:intron	30.0%
!	CTATTTCATTTTCGAACATC+AGG	-	chr4.3:14950035-14950054	MS.gene08545:intron	30.0%
	AGTTCACTTGGCAAATATCA+TGG	-	chr4.3:14949823-14949842	MS.gene08545:intron	35.0%
	CTAAACAACGAGTAAGTTCT+TGG	-	chr4.3:14949731-14949750	MS.gene08545:intron	35.0%
	GAATTTCCTCGTCTAAGAAT+TGG	-	chr4.3:14949551-14949570	MS.gene08545:CDS	35.0%
	GTGATGAATTGGAAACCATT+TGG	-	chr4.3:14949924-14949943	MS.gene08545:intron	35.0%
	TACTAACCAATTCTTAGACG+AGG	+	chr4.3:14949560-14949579	None:intergenic	35.0%
!!	ACAAAAGGTGCATTTTCAAC+AGG	+	chr4.3:14949760-14949779	None:intergenic	35.0%
!!	TTGTTTTGTTGACAGGGATT+GGG	-	chr4.3:14949650-14949669	MS.gene08545:intron	35.0%
!!!	TTTGTTTTGTTGACAGGGAT+TGG	-	chr4.3:14949649-14949668	MS.gene08545:intron	35.0%
	AAACATGTCAACTGATTGGG+TGG	-	chr4.3:14949878-14949897	MS.gene08545:intron	40.0%
	AAATAGAATCAGCTAGTGGC+TGG	+	chr4.3:14950021-14950040	None:intergenic	40.0%
	AAGTGCAGACATTACCAGTT+TGG	-	chr4.3:14950254-14950273	MS.gene08545:CDS	40.0%
	ATCAGGCTACATCCACTATA+TGG	-	chr4.3:14950052-14950071	MS.gene08545:CDS	40.0%
	GTTTGGAAGTGAGTGCAAAA+AGG	-	chr4.3:14950200-14950219	MS.gene08545:intron	40.0%
	TCACTTGGCAAATATCATGG+TGG	-	chr4.3:14949826-14949845	MS.gene08545:intron	40.0%
	TGGAAACCATTTGGGCAAAT+GGG	-	chr4.3:14949933-14949952	MS.gene08545:intron	40.0%
	TTGGAAACCATTTGGGCAAA+TGG	-	chr4.3:14949932-14949951	MS.gene08545:intron	40.0%
	TTGGGTTCTCATAGTTCACT+TGG	-	chr4.3:14949811-14949830	MS.gene08545:intron	40.0%
!!!	GGCTGGTGCTATTTTCATTT+TGG	+	chr4.3:14950004-14950023	None:intergenic	40.0%
	ATTGCTCCCATTTGCCCAAA+TGG	+	chr4.3:14949942-14949961	None:intergenic	45.0%
	GCAGACATTACCAGTTTGGT+AGG	-	chr4.3:14950258-14950277	MS.gene08545:CDS	45.0%
	TCTTGTTGGACAGATTGACG+AGG	-	chr4.3:14950132-14950151	MS.gene08545:intron	45.0%
	TTAGGATCCATTTGTCCAGG+AGG	+	chr4.3:14949679-14949698	None:intergenic	45.0%
!	CTTGGCTCGTTTCCATATAG+TGG	+	chr4.3:14950067-14950086	None:intergenic	45.0%
!!	GCTTTAGGATCCATTTGTCC+AGG	+	chr4.3:14949682-14949701	None:intergenic	45.0%
	ATTGACGAGGCTTTGCACGA+GGG	-	chr4.3:14950145-14950164	MS.gene08545:intron	50.0%
	GATTGACGAGGCTTTGCACG+AGG	-	chr4.3:14950144-14950163	MS.gene08545:intron	55.0%
	TGGGAAGCCTCCTGGACAAA+TGG	-	chr4.3:14949669-14949688	MS.gene08545:intron	55.0%
	TTGACGAGGCTTTGCACGAG+GGG	-	chr4.3:14950146-14950165	MS.gene08545:intron	55.0%
	ACAGGGATTGGGAAGCCTCC+TGG	-	chr4.3:14949661-14949680	MS.gene08545:intron	60.0%
	TGACGAGGCTTTGCACGAGG+GGG	-	chr4.3:14950147-14950166	MS.gene08545:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	14949514	14950279	14949514	ID=MS.gene08545
chr4.3	mRNA	14949514	14950279	14949514	ID=MS.gene08545.t1;Parent=MS.gene08545
chr4.3	exon	14950221	14950279	14950221	ID=MS.gene08545.t1.exon1;Parent=MS.gene08545.t1
chr4.3	CDS	14950221	14950279	14950221	ID=cds.MS.gene08545.t1;Parent=MS.gene08545.t1
chr4.3	exon	14950052	14950128	14950052	ID=MS.gene08545.t1.exon2;Parent=MS.gene08545.t1
chr4.3	CDS	14950052	14950128	14950052	ID=cds.MS.gene08545.t1;Parent=MS.gene08545.t1
chr4.3	exon	14949514	14949648	14949514	ID=MS.gene08545.t1.exon3;Parent=MS.gene08545.t1
chr4.3	CDS	14949514	14949648	14949514	ID=cds.MS.gene08545.t1;Parent=MS.gene08545.t1

Gene Sequence

>MS.gene08545

GCTAAAGAGTGTTATTTATAACTTCAGAGATAATAGTGAATTTCCTCGTCTAAGAATTGGTTAGTATTTTTGTTACAAACTGTTTATTTTTCCCCATGCTTTTTAGATTAATACTGTAATGATTTTTATTTTCAATTTGTTTTGTTGACAGGGATTGGGAAGCCTCCTGGACAAATGGATCCTAAAGCATTTTTGCTTCAAAAGTTTAATGTAACAGCTAAACAACGAGTAAGTTCTTGGTTTCCTGTTGAAAATGCACCTTTTGTTACTCTAGTGTAACTAAGGTTTCAAATTCAATTGGGTTCTCATAGTTCACTTGGCAAATATCATGGTGGTTACTTGTCATGTTAGTGCAATGTCAAAAAAACATGTCAACTGATTGGGTGGCTCTTTAATAATTAAATATTAATGTGATGAATTGGAAACCATTTGGGCAAATGGGAGCAATAGTTATTAGTTGCATCCAACATGAAATTTTCTCGAACATCCAAAATGAAAATAGCACCAGCCACTAGCTGATTCTATTTCATTTTCGAACATCAGGCTACATCCACTATATGGAAACGAGCCAAGTTACAAAAATCTTCATTTGTTCAATGTTTCTTTTTCTCTTATCTTTCTTGTTGGACAGATTGACGAGGCTTTGCACGAGGGGGTTGATGCCTTGAAGCTTCTTCTATTTAAAGGTTTGGAAGTGAGTGCAAAAAGGTTCAACAAAGAACAGAAATATAAGCACTTAAAAGTGCAGACATTACCAGTTTGGTAG

Protein sequence

>MS.gene08545.t1

AKECYLULQRUUUISSSKNWDWEASWTNGSUSIFASKVUCNSUTTNURGFARGGUCLEASSIURFGSECKKVQQRTEIUALKSADITSLV