AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene23422


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23422.t1 MTR_4g094380 41.063 207 65 3 1 150 1 207 3.97e-42 140
MS.gene23422.t1 MTR_4g094380 50.360 139 40 2 1 110 1 139 1.30e-38 129
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23422.t1 AT3G60360 41.262 206 65 4 1 150 1 206 3.83e-40 135

Find 21 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGATTCAGTCTGAGAAAA+AGG 0.320386 7.2:+12750834 MS.gene23422:CDS
ATCTTAAAGTAAAATTCATC+TGG 0.330226 7.2:-12750718 None:intergenic
AATATACCTGAGTCTCTTCC+TGG 0.342208 7.2:-12752378 None:intergenic
AGGGTTTCACGGAAACGATT+TGG 0.362984 7.2:+12750495 MS.gene23422:intron
ATTAGAGCTAGACTTGTTGA+TGG 0.367637 7.2:+12750742 MS.gene23422:CDS
GCTTGTCTGTCAGACACTTA+AGG 0.375501 7.2:+12750675 MS.gene23422:intron
CAGACTGAATCTCTTGAAGA+AGG 0.417743 7.2:-12750825 None:intergenic
GTCTACGGATGATGTTTCTC+AGG 0.432159 7.2:-12750358 None:intergenic
GCACAAAGACTATCTCCTTC+GGG 0.434793 7.2:+12750530 MS.gene23422:CDS
AGCACAAAGACTATCTCCTT+CGG 0.447683 7.2:+12750529 MS.gene23422:CDS
TCGTAACTATTAGGGTTTCA+CGG 0.494684 7.2:+12750484 MS.gene23422:intron
TCTTAAAGTAAAATTCATCT+GGG 0.525519 7.2:-12750717 None:intergenic
AAGAAAGCAAGAGTAGACTC+AGG 0.549205 7.2:+12752323 MS.gene23422:CDS
TGTGATAAGCTTTAGCCCGA+AGG 0.563386 7.2:-12750545 None:intergenic
CTATAACGATTACACAATCC+AGG 0.565748 7.2:+12752360 MS.gene23422:CDS
CACAATCCAGGAAGAGACTC+AGG 0.567026 7.2:+12752372 MS.gene23422:CDS
AGACTGAATCTCTTGAAGAA+GGG 0.587041 7.2:-12750824 None:intergenic
AATAGCATGAGAATTCAGTT+CGG 0.612076 7.2:-12750986 None:intergenic
AGAAAGCAAGAGTAGACTCA+GGG 0.625238 7.2:+12752324 MS.gene23422:CDS
GCTCTTTGTAAGTACGTCTA+CGG 0.634635 7.2:-12750373 None:intergenic
TCTACGGATGATGTTTCTCA+GGG 0.657071 7.2:-12750357 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTCAAAAAAAAAATATGCT+AGG + chr7.2:12751282-12751301 MS.gene23422:intron 15.0%
!! AAAAAAATATGCTAGGAATA+TGG + chr7.2:12751289-12751308 MS.gene23422:intron 20.0%
!! AAAAAATATGCTAGGAATAT+GGG + chr7.2:12751290-12751309 MS.gene23422:intron 20.0%
!! ACCACAAAAATTAATCAAAA+TGG - chr7.2:12752172-12752191 None:intergenic 20.0%
!! ATAAATGTAGAGTAGATAAA+TGG - chr7.2:12751672-12751691 None:intergenic 20.0%
!! ATACGAAATAAAACTTCAAT+AGG - chr7.2:12752041-12752060 None:intergenic 20.0%
!! ATCATGTTTCCTTATTAAAA+CGG + chr7.2:12751735-12751754 MS.gene23422:intron 20.0%
!! ATCTTAAAGTAAAATTCATC+TGG - chr7.2:12750721-12750740 None:intergenic 20.0%
!! TCTTAAAGTAAAATTCATCT+GGG - chr7.2:12750720-12750739 None:intergenic 20.0%
!! TTATGGATCTAACTAAAATT+AGG + chr7.2:12751542-12751561 MS.gene23422:intron 20.0%
!! TTTCTTGAACAAATAAAATG+CGG + chr7.2:12751256-12751275 MS.gene23422:intron 20.0%
!!! AACAAATTTTCAAAAGTTGA+TGG - chr7.2:12751229-12751248 None:intergenic 20.0%
!!! GAACATGAGTTTTATTTTAT+GGG + chr7.2:12751468-12751487 MS.gene23422:intron 20.0%
!!! GCTACTAGTTTTATAATATA+CGG + chr7.2:12752146-12752165 MS.gene23422:intron 20.0%
!!! TCATCTTATTCTATTTTATG+TGG - chr7.2:12751978-12751997 None:intergenic 20.0%
!!! TGAACATGAGTTTTATTTTA+TGG + chr7.2:12751467-12751486 MS.gene23422:intron 20.0%
! ATAGATAAATGGAATAAAGC+CGG + chr7.2:12751814-12751833 MS.gene23422:intron 25.0%
! CATAAATGGAAAGTAAATGT+AGG + chr7.2:12751791-12751810 MS.gene23422:intron 25.0%
! CTTAGTTGTCAATACAATAA+TGG - chr7.2:12751411-12751430 None:intergenic 25.0%
! GAAATAAAACTTCAATAGGA+TGG - chr7.2:12752037-12752056 None:intergenic 25.0%
! GAATATATGATATTGGATCA+GGG - chr7.2:12751370-12751389 None:intergenic 25.0%
! GTAAATGTAGGATAGATAAA+TGG + chr7.2:12751803-12751822 MS.gene23422:intron 25.0%
! TACATGCGAATATATGATAT+TGG - chr7.2:12751377-12751396 None:intergenic 25.0%
! TCAAGTCTTAATGTTAACAA+AGG - chr7.2:12751176-12751195 None:intergenic 25.0%
! TCATGTTTCCTTATTAAAAC+GGG + chr7.2:12751736-12751755 MS.gene23422:intron 25.0%
! TTCATGCTTAGTTTCAAATT+TGG + chr7.2:12750602-12750621 MS.gene23422:intron 25.0%
! TTTCATCTATGAACAGAATA+AGG + chr7.2:12752473-12752492 MS.gene23422:intron 25.0%
!! TAACATGCATGTTTTATGAA+TGG + chr7.2:12751574-12751593 MS.gene23422:intron 25.0%
!!! ATCAATAATTTTAGCCCATA+TGG + chr7.2:12752069-12752088 MS.gene23422:intron 25.0%
!!! GAAGTTTTATTTCGTATAAG+CGG + chr7.2:12752044-12752063 MS.gene23422:intron 25.0%
!!! GCCATTTTGATTAATTTTTG+TGG + chr7.2:12752168-12752187 MS.gene23422:intron 25.0%
!!! GTTTGTTTTCGTAACTATTA+GGG + chr7.2:12750476-12750495 MS.gene23422:intron 25.0%
!!! TTCTCATGCTATTTTCTATA+CGG + chr7.2:12750996-12751015 MS.gene23422:CDS 25.0%
AAAACTTCAATAGGATGGAT+AGG - chr7.2:12752032-12752051 None:intergenic 30.0%
AATAGCATGAGAATTCAGTT+CGG - chr7.2:12750989-12751008 None:intergenic 30.0%
ACAAACGAAATTGAAGCATA+TGG - chr7.2:12750462-12750481 None:intergenic 30.0%
ATCACTCAAACATGTTCATA+AGG - chr7.2:12752416-12752435 None:intergenic 30.0%
ATGTATTGAGTTACTATCCA+AGG - chr7.2:12751623-12751642 None:intergenic 30.0%
CATTCACAAGTAAGCAAATT+TGG - chr7.2:12750926-12750945 None:intergenic 30.0%
CGAATATATGATATTGGATC+AGG - chr7.2:12751371-12751390 None:intergenic 30.0%
GAAGAGAAAGAGAAGAAAAA+AGG + chr7.2:12752091-12752110 MS.gene23422:intron 30.0%
TAAAGCTTATCACAAAAAGC+AGG + chr7.2:12750554-12750573 MS.gene23422:CDS 30.0%
TAGTAACTCAATACATGTGT+TGG + chr7.2:12751627-12751646 MS.gene23422:intron 30.0%
TGACTGATAACAATTATGGT+TGG + chr7.2:12752116-12752135 MS.gene23422:intron 30.0%
TGTATTGAGTTACTATCCAA+GGG - chr7.2:12751622-12751641 None:intergenic 30.0%
TTGTGTCTATCTCTCATAAA+TGG + chr7.2:12751777-12751796 MS.gene23422:intron 30.0%
! TTTTCAGTTTTCTGCAAGAA+GGG - chr7.2:12750958-12750977 None:intergenic 30.0%
!! AAACATGCATGTTATTTCCT+CGG - chr7.2:12751568-12751587 None:intergenic 30.0%
!! ATGATAGATTTTGTTGTGTC+TGG + chr7.2:12751994-12752013 MS.gene23422:intron 30.0%
!! CGTTTGTTTTCGTAACTATT+AGG + chr7.2:12750475-12750494 MS.gene23422:intron 30.0%
!! TAGTAAGACCCGTTTTAATA+AGG - chr7.2:12751747-12751766 None:intergenic 30.0%
!!! ATGCTATTTTCTATACGGAA+AGG + chr7.2:12751001-12751020 MS.gene23422:CDS 30.0%
!!! CATGAGTTTTATTTTATGGG+AGG + chr7.2:12751471-12751490 MS.gene23422:intron 30.0%
AAAGAGAACTAGTTACGGTT+GGG - chr7.2:12750402-12750421 None:intergenic 35.0%
AAAGCCGGAAAGTAAATGTT+GGG + chr7.2:12751829-12751848 MS.gene23422:intron 35.0%
AAATCAACTCATGCTTGTTC+TGG - chr7.2:12751039-12751058 None:intergenic 35.0%
AACGAAATTGAAGCATATGG+AGG - chr7.2:12750459-12750478 None:intergenic 35.0%
ACAAAAAGCAGGACACTTTA+CGG + chr7.2:12750565-12750584 MS.gene23422:CDS 35.0%
AGACACAAGTGAAAGATTAG+TGG + chr7.2:12751701-12751720 MS.gene23422:intron 35.0%
AGACTGAATCTCTTGAAGAA+GGG - chr7.2:12750827-12750846 None:intergenic 35.0%
AGAGATTCAGTCTGAGAAAA+AGG + chr7.2:12750834-12750853 MS.gene23422:CDS 35.0%
ATCTAACTAAAATTAGGCCG+AGG + chr7.2:12751548-12751567 MS.gene23422:intron 35.0%
CATAACTCTACCAAAACATG+GGG - chr7.2:12751527-12751546 None:intergenic 35.0%
CCATAACTCTACCAAAACAT+GGG - chr7.2:12751528-12751547 None:intergenic 35.0%
CTATAACGATTACACAATCC+AGG + chr7.2:12752360-12752379 MS.gene23422:CDS 35.0%
CTTACTTGTGAATGTCTTGT+TGG + chr7.2:12750932-12750951 MS.gene23422:intron 35.0%
CTTCTCTTTCTCTTCCATAT+GGG - chr7.2:12752086-12752105 None:intergenic 35.0%
GAGAACAATCTATCATTCTG+TGG - chr7.2:12752291-12752310 None:intergenic 35.0%
GAGAGAAAGAGAACTAGTTA+CGG - chr7.2:12750407-12750426 None:intergenic 35.0%
GGAGTGACTGATAACAATTA+TGG + chr7.2:12752112-12752131 MS.gene23422:intron 35.0%
TAAAGCCGGAAAGTAAATGT+TGG + chr7.2:12751828-12751847 MS.gene23422:intron 35.0%
TATCCTGACATTCAACATCA+CGG + chr7.2:12750778-12750797 MS.gene23422:intron 35.0%
TCCATAACTCTACCAAAACA+TGG - chr7.2:12751529-12751548 None:intergenic 35.0%
TCGTAACTATTAGGGTTTCA+CGG + chr7.2:12750484-12750503 MS.gene23422:intron 35.0%
TCTTCTCTTTCTCTTCCATA+TGG - chr7.2:12752087-12752106 None:intergenic 35.0%
TTGACAACTAAGATAGCATC+TGG + chr7.2:12751419-12751438 MS.gene23422:intron 35.0%
TTGTTCAATGAAAGCTGACT+TGG + chr7.2:12752212-12752231 MS.gene23422:intron 35.0%
! AAGGATAAAAAGCGCAAACT+AGG + chr7.2:12752492-12752511 MS.gene23422:CDS 35.0%
! CTTTTCAGTTTTCTGCAAGA+AGG - chr7.2:12750959-12750978 None:intergenic 35.0%
!! ATTAGAGCTAGACTTGTTGA+TGG + chr7.2:12750742-12750761 MS.gene23422:CDS 35.0%
AAGAAAGCAAGAGTAGACTC+AGG + chr7.2:12752323-12752342 MS.gene23422:CDS 40.0%
AATATACCTGAGTCTCTTCC+TGG - chr7.2:12752381-12752400 None:intergenic 40.0%
AATCTCTTGAAGAAGGGTTC+TGG - chr7.2:12750821-12750840 None:intergenic 40.0%
AGAAAGCAAGAGTAGACTCA+GGG + chr7.2:12752324-12752343 MS.gene23422:CDS 40.0%
AGCACAAAGACTATCTCCTT+CGG + chr7.2:12750529-12750548 MS.gene23422:CDS 40.0%
ATCCTGACATTCAACATCAC+GGG + chr7.2:12750779-12750798 MS.gene23422:intron 40.0%
CAAAAAGCAGGACACTTTAC+GGG + chr7.2:12750566-12750585 MS.gene23422:CDS 40.0%
CAGACTGAATCTCTTGAAGA+AGG - chr7.2:12750828-12750847 None:intergenic 40.0%
GAAAGAGAACTAGTTACGGT+TGG - chr7.2:12750403-12750422 None:intergenic 40.0%
GATTACAGTCAACTGAGTCA+AGG - chr7.2:12751346-12751365 None:intergenic 40.0%
GATTCAGTCTGAGAAAAAGG+TGG + chr7.2:12750837-12750856 MS.gene23422:intron 40.0%
GCTCTTTGTAAGTACGTCTA+CGG - chr7.2:12750376-12750395 None:intergenic 40.0%
TATGCTAGGAATATGGGTTC+AGG + chr7.2:12751296-12751315 MS.gene23422:intron 40.0%
TCTACGGATGATGTTTCTCA+GGG - chr7.2:12750360-12750379 None:intergenic 40.0%
! GACTTGGTTCATAGTGCATT+TGG + chr7.2:12752228-12752247 MS.gene23422:intron 40.0%
!!! CCCATGTTTTGGTAGAGTTA+TGG + chr7.2:12751525-12751544 MS.gene23422:intron 40.0%
ACCAAAACATGGGGACATAC+TGG - chr7.2:12751518-12751537 None:intergenic 45.0%
AGGGTTTCACGGAAACGATT+TGG + chr7.2:12750495-12750514 MS.gene23422:intron 45.0%
CAGTCTGAGAAAAAGGTGGA+TGG + chr7.2:12750841-12750860 MS.gene23422:intron 45.0%
GCACAAAGACTATCTCCTTC+GGG + chr7.2:12750530-12750549 MS.gene23422:CDS 45.0%
GCTGCCCAACATTTACTTTC+CGG - chr7.2:12751836-12751855 None:intergenic 45.0%
GCTTGTCTGTCAGACACTTA+AGG + chr7.2:12750675-12750694 MS.gene23422:intron 45.0%
GTCTACGGATGATGTTTCTC+AGG - chr7.2:12750361-12750380 None:intergenic 45.0%
TAAGATAGCATCTGGCAGAC+AGG + chr7.2:12751427-12751446 MS.gene23422:intron 45.0%
TCAACTGAGTCAAGGCTTCA+TGG - chr7.2:12751338-12751357 None:intergenic 45.0%
TCTGGCAGACAGGAAAAAGT+AGG + chr7.2:12751437-12751456 MS.gene23422:intron 45.0%
TTCCCGTGATGTTGAATGTC+AGG - chr7.2:12750784-12750803 None:intergenic 45.0%
! ACTCAATACATGTGTTGGCG+AGG + chr7.2:12751632-12751651 MS.gene23422:intron 45.0%
! TATCCAAGGGTACGCTGAAA+AGG - chr7.2:12751609-12751628 None:intergenic 45.0%
! TCCAGTATGTCCCCATGTTT+TGG + chr7.2:12751514-12751533 MS.gene23422:intron 45.0%
! TGTGATAAGCTTTAGCCCGA+AGG - chr7.2:12750548-12750567 None:intergenic 45.0%
CACAATCCAGGAAGAGACTC+AGG + chr7.2:12752372-12752391 MS.gene23422:CDS 50.0%
! AGTCCTTTTCAGCGTACCCT+TGG + chr7.2:12751603-12751622 MS.gene23422:intron 50.0%
! CATGTGTTGGCGAGGAGTTC+TGG + chr7.2:12751640-12751659 MS.gene23422:intron 55.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 12750350 12752530 12750350 ID=MS.gene23422
chr7.2 mRNA 12750350 12752530 12750350 ID=MS.gene23422.t1;Parent=MS.gene23422
chr7.2 exon 12750350 12750405 12750350 ID=MS.gene23422.t1.exon1;Parent=MS.gene23422.t1
chr7.2 CDS 12750350 12750405 12750350 ID=cds.MS.gene23422.t1;Parent=MS.gene23422.t1
chr7.2 exon 12750497 12750587 12750497 ID=MS.gene23422.t1.exon2;Parent=MS.gene23422.t1
chr7.2 CDS 12750497 12750587 12750497 ID=cds.MS.gene23422.t1;Parent=MS.gene23422.t1
chr7.2 exon 12750688 12750776 12750688 ID=MS.gene23422.t1.exon3;Parent=MS.gene23422.t1
chr7.2 CDS 12750688 12750776 12750688 ID=cds.MS.gene23422.t1;Parent=MS.gene23422.t1
chr7.2 exon 12750822 12750855 12750822 ID=MS.gene23422.t1.exon4;Parent=MS.gene23422.t1
chr7.2 CDS 12750822 12750855 12750822 ID=cds.MS.gene23422.t1;Parent=MS.gene23422.t1
chr7.2 exon 12750967 12751022 12750967 ID=MS.gene23422.t1.exon5;Parent=MS.gene23422.t1
chr7.2 CDS 12750967 12751022 12750967 ID=cds.MS.gene23422.t1;Parent=MS.gene23422.t1
chr7.2 exon 12752294 12752393 12752294 ID=MS.gene23422.t1.exon6;Parent=MS.gene23422.t1
chr7.2 CDS 12752294 12752393 12752294 ID=cds.MS.gene23422.t1;Parent=MS.gene23422.t1
chr7.2 exon 12752489 12752530 12752489 ID=MS.gene23422.t1.exon7;Parent=MS.gene23422.t1
chr7.2 CDS 12752489 12752530 12752489 ID=cds.MS.gene23422.t1;Parent=MS.gene23422.t1
Gene Sequence

>MS.gene23422

ATGGCTTCCCTGAGAAACATCATCCGTAGACGTACTTACAAAGAGCGTGCCCAACCGTAACTAGTTCTCTTTCTCTCACAATTATCGTTGATATGCTCTGTATCTGCCTCCATATGCTTCAATTTCGTTTGTTTTCGTAACTATTAGGGTTTCACGGAAACGATTTGGAATACTCGAAAAGCACAAAGACTATCTCCTTCGGGCTAAAGCTTATCACAAAAAGCAGGACACTTTACGGGTATTTTATTTTCATTCATGCTTAGTTTCAAATTTGGTGATTTTGATGATAGTTTCTATCTTTAAATCATCGTTTTATCTATTATTTGCTTGTCTGTCAGACACTTAAGGAAAAAGCTGCAAATAGAAACCCAGATGAATTTTACTTTAAGATGATTAGAGCTAGACTTGTTGATGGAGTTCATACTAAGTATCCTGACATTCAACATCACGGGAAACATGACAATCAGACCAGAACCCTTCTTCAAGAGATTCAGTCTGAGAAAAAGGTGGATGGTTTATTTGTCTCTTTTTTTTTTTTTTTTTTTTGTAATATTTATTGTTGCGCTGTATATGCCAAATTTGCTTACTTGTGAATGTCTTGTTGGCCCTTCTTGCAGAAAACTGAAAAGATAAACGCCGAACTGAATTCTCATGCTATTTTCTATACGGAAAGGTTTGAGTTTCTACCAGAACAAGCATGAGTTGATTTTGTTTGTCAGATATATGTGCTTATTTCTTTTTAGTTCTTCAAGTGTTTCAACTTTTTTTCGTTGTTGTGCTTTTATATTTTATATTTTTACAAATGCTCAGATTTGATGAACATCCTTTGTTAACATTAAGACTTGATTGAAGTTTCTTTTGTCTGTTGTCTTTTGTCCATCAACTTTTGAAAATTTGTTTAGTGTATTTCTTGAACAAATAAAATGCGGTTGTTTCAAAAAAAAAATATGCTAGGAATATGGGTTCAGGAATCATGCTATTTTTTCCATGAAGCCTTGACTCAGTTGACTGTAATCACCCTGATCCAATATCATATATTCGCATGTATGTTTGTTGTACCATTATTGTATTGACAACTAAGATAGCATCTGGCAGACAGGAAAAAGTAGGACAAAATTGAACATGAGTTTTATTTTATGGGAGGTGCGAAATAACTATATCTTCTCCAGTATGTCCCCATGTTTTGGTAGAGTTATGGATCTAACTAAAATTAGGCCGAGGAAATAACATGCATGTTTTATGAATGGTTTGCTAGTCCTTTTCAGCGTACCCTTGGATAGTAACTCAATACATGTGTTGGCGAGGAGTTCTGGTTTGCTCCATTTATCTACTCTACATTTATGAGATAGATAGACACAAGTGAAAGATTAGTGGCAGATTAGTAAATCATGTTTCCTTATTAAAACGGGTCTTACTAATTCTATCACTTGTGTCTATCTCTCATAAATGGAAAGTAAATGTAGGATAGATAAATGGAATAAAGCCGGAAAGTAAATGTTGGGCAGCTATAGAGTATAGAAAGCTATGTGAGTTTGTACTCAATAAATATTATATAGCATTTCGCTTTGATGTTCAAAAACAAGTTATGTTTTGTTAGATACATATTAAAACAAGTTATATATATGCCACATAAAATAGAATAAGATGATAGATTTTGTTGTGTCTGGTGTCAAAAGCTTCCTATCCATCCTATTGAAGTTTTATTTCGTATAAGCGGTGATCAATAATTTTAGCCCATATGGAAGAGAAAGAGAAGAAAAAAGGAGTGACTGATAACAATTATGGTTGGATTAGTTGCTACTAGTTTTATAATATACGGCCATTTTGATTAATTTTTGTGGTATGTTGATTTTGTTGTGATATTGTTCAATGAAAGCTGACTTGGTTCATAGTGCATTTGGCTGTCGTCATGAAATAAACATAGTTATCTCATTTCATCCACAGAATGATAGATTGTTCTCGTAGACAGTTGAAAGAAAGCAAGAGTAGACTCAGGGAGCTAGAGAAAATCTATAACGATTACACAATCCAGGAAGAGACTCAGGTATATTTATTTTTAATTTCCTTATGAACATGTTTGAGTGATCGAGTCTTATATTTATGTATATAGACTCAGAAAATAATTTCATCTATGAACAGAATAAGGATAAAAAGCGCAAACTAGGCAAAAGAAAGCGTTGA

Protein sequence

>MS.gene23422.t1

MASLRNIIRRRTYKERAQPVSRKRFGILEKHKDYLLRAKAYHKKQDTLRTLKEKAANRNPDEFYFKMIRARLVDGVHTKTLLQEIQSEKKKTEKINAELNSHAIFYTERMIDCSRRQLKESKSRLRELEKIYNDYTIQEETQNKDKKRKLGKRKR