Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24359.t1 | XP_003589248.1 | 71.1 | 304 | 86 | 1 | 1 | 304 | 1 | 302 | 9.20E-78 | 300.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24359.t1 | G7I4I2 | 71.1 | 304 | 86 | 1 | 1 | 304 | 1 | 302 | 6.6e-78 | 300.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049217 | MS.gene24359 | -0.807536 | 4.82E-50 | -1.69E-46 |
| MS.gene049281 | MS.gene24359 | 0.821006 | 5.08E-53 | -1.69E-46 |
| MS.gene049285 | MS.gene24359 | -0.808433 | 3.11E-50 | -1.69E-46 |
| MS.gene049320 | MS.gene24359 | -0.80826 | 3.38E-50 | -1.69E-46 |
| MS.gene050173 | MS.gene24359 | 0.816903 | 4.35E-52 | -1.69E-46 |
| MS.gene050216 | MS.gene24359 | -0.805104 | 1.57E-49 | -1.69E-46 |
| MS.gene050363 | MS.gene24359 | 0.901225 | 3.32E-78 | -1.69E-46 |
| MS.gene050381 | MS.gene24359 | 0.875661 | 2.58E-68 | -1.69E-46 |
| MS.gene050416 | MS.gene24359 | 0.814909 | 1.21E-51 | -1.69E-46 |
| MS.gene050504 | MS.gene24359 | -0.818759 | 1.66E-52 | -1.69E-46 |
| MS.gene050612 | MS.gene24359 | -0.831321 | 1.78E-55 | -1.69E-46 |
| MS.gene050675 | MS.gene24359 | -0.802663 | 5.04E-49 | -1.69E-46 |
| MS.gene050883 | MS.gene24359 | 0.818164 | 2.26E-52 | -1.69E-46 |
| MS.gene050986 | MS.gene24359 | -0.805098 | 1.57E-49 | -1.69E-46 |
| MS.gene051044 | MS.gene24359 | 0.809264 | 2.06E-50 | -1.69E-46 |
| MS.gene051127 | MS.gene24359 | 0.871143 | 8.56E-67 | -1.69E-46 |
| MS.gene051138 | MS.gene24359 | 0.807435 | 5.06E-50 | -1.69E-46 |
| MS.gene05141 | MS.gene24359 | 0.807288 | 5.44E-50 | -1.69E-46 |
| MS.gene051564 | MS.gene24359 | -0.804812 | 1.81E-49 | -1.69E-46 |
| MS.gene051810 | MS.gene24359 | -0.825764 | 3.92E-54 | -1.69E-46 |
| MS.gene051946 | MS.gene24359 | 0.849475 | 3.16E-60 | -1.69E-46 |
| MS.gene053420 | MS.gene24359 | 0.835158 | 1.97E-56 | -1.69E-46 |
| MS.gene053488 | MS.gene24359 | 0.82692 | 2.08E-54 | -1.69E-46 |
| MS.gene053561 | MS.gene24359 | 0.909051 | 8.72E-82 | -1.69E-46 |
| MS.gene054056 | MS.gene24359 | 0.804086 | 2.56E-49 | -1.69E-46 |
| MS.gene054616 | MS.gene24359 | 0.834688 | 2.59E-56 | -1.69E-46 |
| MS.gene054626 | MS.gene24359 | 0.812477 | 4.16E-51 | -1.69E-46 |
| MS.gene054642 | MS.gene24359 | -0.819056 | 1.42E-52 | -1.69E-46 |
| MS.gene054708 | MS.gene24359 | 0.83631 | 1.01E-56 | -1.69E-46 |
| MS.gene054954 | MS.gene24359 | -0.804415 | 2.18E-49 | -1.69E-46 |
| MS.gene054959 | MS.gene24359 | 0.827446 | 1.56E-54 | -1.69E-46 |
| MS.gene055124 | MS.gene24359 | 0.820014 | 8.58E-53 | -1.69E-46 |
| MS.gene055154 | MS.gene24359 | 0.857948 | 1.15E-62 | -1.69E-46 |
| MS.gene055202 | MS.gene24359 | 0.831781 | 1.37E-55 | -1.69E-46 |
| MS.gene055362 | MS.gene24359 | -0.802674 | 5.02E-49 | -1.69E-46 |
| MS.gene055502 | MS.gene24359 | -0.814462 | 1.52E-51 | -1.69E-46 |
| MS.gene055577 | MS.gene24359 | -0.807305 | 5.39E-50 | -1.69E-46 |
| MS.gene056310 | MS.gene24359 | 0.80762 | 4.63E-50 | -1.69E-46 |
| MS.gene056311 | MS.gene24359 | 0.832406 | 9.63E-56 | -1.69E-46 |
| MS.gene056313 | MS.gene24359 | 0.826774 | 2.26E-54 | -1.69E-46 |
| MS.gene056408 | MS.gene24359 | 0.821047 | 4.97E-53 | -1.69E-46 |
| MS.gene057014 | MS.gene24359 | 0.835147 | 1.99E-56 | -1.69E-46 |
| MS.gene057473 | MS.gene24359 | 0.818925 | 1.52E-52 | -1.69E-46 |
| MS.gene058175 | MS.gene24359 | 0.800952 | 1.13E-48 | -1.69E-46 |
| MS.gene058628 | MS.gene24359 | 0.805394 | 1.36E-49 | -1.69E-46 |
| MS.gene058630 | MS.gene24359 | 0.803967 | 2.71E-49 | -1.69E-46 |
| MS.gene058773 | MS.gene24359 | -0.817795 | 2.74E-52 | -1.69E-46 |
| MS.gene058840 | MS.gene24359 | 0.842959 | 1.88E-58 | -1.69E-46 |
| MS.gene058978 | MS.gene24359 | 0.83553 | 1.59E-56 | -1.69E-46 |
| MS.gene059022 | MS.gene24359 | -0.836584 | 8.59E-57 | -1.69E-46 |
| MS.gene059690 | MS.gene24359 | 0.859235 | 4.76E-63 | -1.69E-46 |
| MS.gene059691 | MS.gene24359 | 0.843869 | 1.07E-58 | -1.69E-46 |
| MS.gene059694 | MS.gene24359 | 0.857453 | 1.62E-62 | -1.69E-46 |
| MS.gene059697 | MS.gene24359 | 0.860125 | 2.57E-63 | -1.69E-46 |
| MS.gene059865 | MS.gene24359 | -0.815519 | 8.87E-52 | -1.69E-46 |
| MS.gene059902 | MS.gene24359 | -0.800223 | 1.59E-48 | -1.69E-46 |
| MS.gene059916 | MS.gene24359 | 0.84438 | 7.84E-59 | -1.69E-46 |
| MS.gene059943 | MS.gene24359 | 0.802161 | 6.40E-49 | -1.69E-46 |
| MS.gene06012 | MS.gene24359 | 0.803575 | 3.26E-49 | -1.69E-46 |
| MS.gene060703 | MS.gene24359 | -0.804761 | 1.85E-49 | -1.69E-46 |
| MS.gene06074 | MS.gene24359 | 0.832428 | 9.51E-56 | -1.69E-46 |
| MS.gene06077 | MS.gene24359 | -0.803947 | 2.73E-49 | -1.69E-46 |
| MS.gene060808 | MS.gene24359 | 0.805371 | 1.38E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24359.t1 | MTR_1g021070 | 95.082 | 305 | 11 | 2 | 1 | 304 | 1 | 302 | 0.0 | 575 |
| MS.gene24359.t1 | MTR_3g101320 | 49.701 | 334 | 98 | 14 | 1 | 303 | 1 | 295 | 5.55e-78 | 239 |
| MS.gene24359.t1 | MTR_4g086620 | 39.130 | 322 | 152 | 8 | 1 | 299 | 1 | 301 | 1.64e-53 | 177 |
| MS.gene24359.t1 | MTR_1g033900 | 37.692 | 260 | 121 | 10 | 57 | 302 | 28 | 260 | 1.13e-35 | 130 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24359.t1 | AT2G15760 | 39.274 | 303 | 153 | 12 | 13 | 302 | 13 | 297 | 1.13e-29 | 115 |
| MS.gene24359.t1 | AT2G26530 | 34.219 | 301 | 156 | 10 | 25 | 300 | 6 | 289 | 1.85e-25 | 103 |
| MS.gene24359.t1 | AT2G26530 | 32.622 | 328 | 152 | 10 | 25 | 300 | 6 | 316 | 2.36e-21 | 92.4 |
Find 38 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCTTATAGACAAGGGATTT+TGG | 0.133320 | 1.1:-3067966 | MS.gene24359:CDS |
| CCCAAAATCCCTTGTCTATA+AGG | 0.209177 | 1.1:+3067965 | None:intergenic |
| TGATGATGTTTGATCAATTA+TGG | 0.230244 | 1.1:+3068657 | None:intergenic |
| TTAGCTTCTTCATTAGTAAA+TGG | 0.259977 | 1.1:+3068496 | None:intergenic |
| TTGGGAAGGTTGTCTGGATT+TGG | 0.260298 | 1.1:-3067947 | MS.gene24359:CDS |
| CCATCTTCTCCAAAACGTTT+TGG | 0.280265 | 1.1:-3068751 | MS.gene24359:CDS |
| TAAAATTCACCAAAACGTTT+TGG | 0.288002 | 1.1:+3068742 | None:intergenic |
| TCGACGATTTATTTGATTCA+TGG | 0.314817 | 1.1:+3068560 | None:intergenic |
| GATGATACAAAGTTCTAGTA+TGG | 0.343620 | 1.1:-3068824 | MS.gene24359:CDS |
| AATACAAGTGAAGAAGTTAA+AGG | 0.350409 | 1.1:-3068121 | MS.gene24359:CDS |
| GAATCATCATCAAATAATTC+TGG | 0.360745 | 1.1:-3068406 | MS.gene24359:CDS |
| GATTCCTCTTTGTTGCTACT+AGG | 0.362236 | 1.1:+3068295 | None:intergenic |
| TTCTGAATTATATAGTGAAA+TGG | 0.377274 | 1.1:-3068689 | MS.gene24359:CDS |
| TTTCGAAGGGTTCGAGGAGA+TGG | 0.388899 | 1.1:-3068237 | MS.gene24359:CDS |
| CCAACAAAATATTTCTTCAA+TGG | 0.389000 | 1.1:+3068154 | None:intergenic |
| TATAATTCAGAAAGCCTAGA+TGG | 0.397292 | 1.1:+3068700 | None:intergenic |
| TCAGCTGAAGAACTCTTTCA+TGG | 0.412277 | 1.1:-3068532 | MS.gene24359:CDS |
| GAAAGCCTAGATGGACTTGA+AGG | 0.448937 | 1.1:+3068709 | None:intergenic |
| GTGCCCCTTCAAGTCCATCT+AGG | 0.450184 | 1.1:-3068714 | MS.gene24359:CDS |
| GTTCGATTTCGAAGGGTTCG+AGG | 0.475655 | 1.1:-3068243 | MS.gene24359:CDS |
| AAGATATCAAACAGTAGTAC+AGG | 0.498075 | 1.1:-3068271 | MS.gene24359:CDS |
| AGGTCCTGGTTCGATTTCGA+AGG | 0.504546 | 1.1:-3068251 | MS.gene24359:CDS |
| GAACCCTTCGAAATCGAACC+AGG | 0.510031 | 1.1:+3068247 | None:intergenic |
| AGAAGTGCTTCAGAAGGAAG+AGG | 0.521434 | 1.1:-3068190 | MS.gene24359:CDS |
| TCAAACAGTAGTACAGGTCC+TGG | 0.538921 | 1.1:-3068265 | MS.gene24359:CDS |
| ATTTACTAATGAAGAAGCTA+AGG | 0.540876 | 1.1:-3068494 | MS.gene24359:CDS |
| AAAGTTCAAGATGAAAGAAG+AGG | 0.559986 | 1.1:-3068448 | MS.gene24359:CDS |
| GGTCCTGGTTCGATTTCGAA+GGG | 0.575115 | 1.1:-3068250 | MS.gene24359:CDS |
| GAAGATGGAGGACTTAGATA+CGG | 0.590817 | 1.1:+3068766 | None:intergenic |
| AAAGCCTAGATGGACTTGAA+GGG | 0.592999 | 1.1:+3068710 | None:intergenic |
| CTTTCTTGCCTTATAGACAA+GGG | 0.596379 | 1.1:-3067973 | MS.gene24359:CDS |
| ACTTTCTTGCCTTATAGACA+AGG | 0.596868 | 1.1:-3067974 | MS.gene24359:CDS |
| TTGTTTAGAAGTGCTTCAGA+AGG | 0.602019 | 1.1:-3068196 | MS.gene24359:CDS |
| ACAACCTAGTAGCAACAAAG+AGG | 0.606605 | 1.1:-3068299 | MS.gene24359:CDS |
| AAGCCTAGATGGACTTGAAG+GGG | 0.623098 | 1.1:+3068711 | None:intergenic |
| AGAAGGAAGAGGTTCAAGCA+AGG | 0.639581 | 1.1:-3068179 | MS.gene24359:CDS |
| GCTGAAGAACTCTTTCATGG+TGG | 0.696192 | 1.1:-3068529 | MS.gene24359:CDS |
| GATGATGATGATAAGCAAGG+TGG | 0.748240 | 1.1:-3068601 | MS.gene24359:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTCTGAATTATATAGTGAAA+TGG | - | chr1.1:3068083-3068102 | MS.gene24359:CDS | 20.0% |
| !!! | ATGTAGTTTGATTTTCTATA+AGG | + | chr1.1:3068417-3068436 | None:intergenic | 20.0% |
| !!! | CATTGAAGAAATATTTTGTT+GGG | - | chr1.1:3068619-3068638 | MS.gene24359:CDS | 20.0% |
| ! | AATACAAGTGAAGAAGTTAA+AGG | - | chr1.1:3068651-3068670 | MS.gene24359:CDS | 25.0% |
| ! | ATTTACTAATGAAGAAGCTA+AGG | - | chr1.1:3068278-3068297 | MS.gene24359:CDS | 25.0% |
| ! | CCAACAAAATATTTCTTCAA+TGG | + | chr1.1:3068621-3068640 | None:intergenic | 25.0% |
| ! | GAATCATCATCAAATAATTC+TGG | - | chr1.1:3068366-3068385 | MS.gene24359:CDS | 25.0% |
| ! | TTAGCTTCTTCATTAGTAAA+TGG | + | chr1.1:3068279-3068298 | None:intergenic | 25.0% |
| !! | CCATTGAAGAAATATTTTGT+TGG | - | chr1.1:3068618-3068637 | MS.gene24359:CDS | 25.0% |
| !! | TAAAATTCACCAAAACGTTT+TGG | + | chr1.1:3068033-3068052 | None:intergenic | 25.0% |
| !! | TGATGATGTTTGATCAATTA+TGG | + | chr1.1:3068118-3068137 | None:intergenic | 25.0% |
| !!! | GATACATTCAATTTTAGTAG+TGG | - | chr1.1:3067970-3067989 | MS.gene24359:CDS | 25.0% |
| AAAGTTCAAGATGAAAGAAG+AGG | - | chr1.1:3068324-3068343 | MS.gene24359:CDS | 30.0% | |
| AAGATATCAAACAGTAGTAC+AGG | - | chr1.1:3068501-3068520 | MS.gene24359:CDS | 30.0% | |
| TATAATTCAGAAAGCCTAGA+TGG | + | chr1.1:3068075-3068094 | None:intergenic | 30.0% | |
| TCGACGATTTATTTGATTCA+TGG | + | chr1.1:3068215-3068234 | None:intergenic | 30.0% | |
| ! | GATGATACAAAGTTCTAGTA+TGG | - | chr1.1:3067948-3067967 | MS.gene24359:CDS | 30.0% |
| !! | TGAACTTTTTGTTGCTTCTT+AGG | + | chr1.1:3068312-3068331 | None:intergenic | 30.0% |
| !!! | ATGAACAACGTTGTTGTTTT+TGG | + | chr1.1:3068142-3068161 | None:intergenic | 30.0% |
| ACTTTCTTGCCTTATAGACA+AGG | - | chr1.1:3068798-3068817 | MS.gene24359:CDS | 35.0% | |
| CTTTCTTGCCTTATAGACAA+GGG | - | chr1.1:3068799-3068818 | MS.gene24359:CDS | 35.0% | |
| ! | CCTTATAGACAAGGGATTTT+GGG | - | chr1.1:3068807-3068826 | MS.gene24359:CDS | 35.0% |
| ! | GATGATGATGATGATAAGCA+AGG | - | chr1.1:3068168-3068187 | MS.gene24359:CDS | 35.0% |
| !! | CTGATTCGTTTTCACATCAT+GGG | - | chr1.1:3068694-3068713 | MS.gene24359:CDS | 35.0% |
| !! | TCTGATTCGTTTTCACATCA+TGG | - | chr1.1:3068693-3068712 | MS.gene24359:CDS | 35.0% |
| !! | TTGTTTAGAAGTGCTTCAGA+AGG | - | chr1.1:3068576-3068595 | MS.gene24359:CDS | 35.0% |
| ACAACCTAGTAGCAACAAAG+AGG | - | chr1.1:3068473-3068492 | MS.gene24359:CDS | 40.0% | |
| ACATCATGGGTCGAGAAAAA+AGG | - | chr1.1:3068707-3068726 | MS.gene24359:CDS | 40.0% | |
| CCCAAAATCCCTTGTCTATA+AGG | + | chr1.1:3068810-3068829 | None:intergenic | 40.0% | |
| GAAGATGGAGGACTTAGATA+CGG | + | chr1.1:3068009-3068028 | None:intergenic | 40.0% | |
| GATTCCTCTTTGTTGCTACT+AGG | + | chr1.1:3068480-3068499 | None:intergenic | 40.0% | |
| TCAGCTGAAGAACTCTTTCA+TGG | - | chr1.1:3068240-3068259 | MS.gene24359:CDS | 40.0% | |
| ! | AAAGCCTAGATGGACTTGAA+GGG | + | chr1.1:3068065-3068084 | None:intergenic | 40.0% |
| ! | CCATCTTCTCCAAAACGTTT+TGG | - | chr1.1:3068021-3068040 | MS.gene24359:CDS | 40.0% |
| ! | GATGATGATGATAAGCAAGG+TGG | - | chr1.1:3068171-3068190 | MS.gene24359:CDS | 40.0% |
| ! | GCCTTATAGACAAGGGATTT+TGG | - | chr1.1:3068806-3068825 | MS.gene24359:CDS | 40.0% |
| !! | ATAGACAAGGGATTTTGGGA+AGG | - | chr1.1:3068811-3068830 | MS.gene24359:CDS | 40.0% |
| !!! | AAACGTTTTGGAGAAGATGG+AGG | + | chr1.1:3068021-3068040 | None:intergenic | 40.0% |
| !!! | CCAAAACGTTTTGGAGAAGA+TGG | + | chr1.1:3068024-3068043 | None:intergenic | 40.0% |
| AGAAGGAAGAGGTTCAAGCA+AGG | - | chr1.1:3068593-3068612 | MS.gene24359:CDS | 45.0% | |
| GCTGAAGAACTCTTTCATGG+TGG | - | chr1.1:3068243-3068262 | MS.gene24359:CDS | 45.0% | |
| TCAAACAGTAGTACAGGTCC+TGG | - | chr1.1:3068507-3068526 | MS.gene24359:CDS | 45.0% | |
| TTGGGAAGGTTGTCTGGATT+TGG | - | chr1.1:3068825-3068844 | MS.gene24359:CDS | 45.0% | |
| ! | AAGCCTAGATGGACTTGAAG+GGG | + | chr1.1:3068064-3068083 | None:intergenic | 45.0% |
| ! | GAAAGCCTAGATGGACTTGA+AGG | + | chr1.1:3068066-3068085 | None:intergenic | 45.0% |
| !! | AGAAGTGCTTCAGAAGGAAG+AGG | - | chr1.1:3068582-3068601 | MS.gene24359:CDS | 45.0% |
| GAACCCTTCGAAATCGAACC+AGG | + | chr1.1:3068528-3068547 | None:intergenic | 50.0% | |
| GTTCGATTTCGAAGGGTTCG+AGG | - | chr1.1:3068529-3068548 | MS.gene24359:CDS | 50.0% | |
| TTTCGAAGGGTTCGAGGAGA+TGG | - | chr1.1:3068535-3068554 | MS.gene24359:CDS | 50.0% | |
| ! | AGGTCCTGGTTCGATTTCGA+AGG | - | chr1.1:3068521-3068540 | MS.gene24359:CDS | 50.0% |
| ! | GGTCCTGGTTCGATTTCGAA+GGG | - | chr1.1:3068522-3068541 | MS.gene24359:CDS | 50.0% |
| !! | GGGATTTTGGGAAGGTTGTC+TGG | - | chr1.1:3068819-3068838 | MS.gene24359:CDS | 50.0% |
| GTGCCCCTTCAAGTCCATCT+AGG | - | chr1.1:3068058-3068077 | MS.gene24359:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 3067940 | 3068854 | 3067940 | ID=MS.gene24359 |
| chr1.1 | mRNA | 3067940 | 3068854 | 3067940 | ID=MS.gene24359.t1;Parent=MS.gene24359 |
| chr1.1 | exon | 3067940 | 3068854 | 3067940 | ID=MS.gene24359.t1.exon1;Parent=MS.gene24359.t1 |
| chr1.1 | CDS | 3067940 | 3068854 | 3067940 | ID=cds.MS.gene24359.t1;Parent=MS.gene24359.t1 |
| Gene Sequence |
| Protein sequence |
