AlfalfaGEDB | MS.gene28864

M. sativa cultivar XinJiangDaYe / MS.gene28864

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28864.t1	XP_024627727.1	98.8	82	1	0	1	82	7	88	5.20E-36	159.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28864.t1	G7LB79	98.8	82	1	0	1	82	7	88	3.8e-36	159.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene28864	0.831789	1.37E-55	-1.69E-46
MS.gene049255	MS.gene28864	0.833345	5.63E-56	-1.69E-46
MS.gene050277	MS.gene28864	0.801938	7.11E-49	-1.69E-46
MS.gene050303	MS.gene28864	0.825285	5.09E-54	-1.69E-46
MS.gene050605	MS.gene28864	0.804638	1.96E-49	-1.69E-46
MS.gene050689	MS.gene28864	0.810168	1.32E-50	-1.69E-46
MS.gene050807	MS.gene28864	0.841959	3.46E-58	-1.69E-46
MS.gene050815	MS.gene28864	0.825614	4.26E-54	-1.69E-46
MS.gene050987	MS.gene28864	0.831566	1.55E-55	-1.69E-46
MS.gene051180	MS.gene28864	0.815795	7.70E-52	-1.69E-46
MS.gene051243	MS.gene28864	0.838133	3.45E-57	-1.69E-46
MS.gene051328	MS.gene28864	0.809787	1.59E-50	-1.69E-46
MS.gene05134	MS.gene28864	0.835194	1.93E-56	-1.69E-46
MS.gene051451	MS.gene28864	0.805754	1.15E-49	-1.69E-46
MS.gene051535	MS.gene28864	0.80875	2.66E-50	-1.69E-46
MS.gene052012	MS.gene28864	0.808977	2.38E-50	-1.69E-46
MS.gene052286	MS.gene28864	0.810111	1.36E-50	-1.69E-46
MS.gene052426	MS.gene28864	0.80807	3.71E-50	-1.69E-46
MS.gene052954	MS.gene28864	0.802784	4.76E-49	-1.69E-46
MS.gene053006	MS.gene28864	0.806524	7.89E-50	-1.69E-46
MS.gene053008	MS.gene28864	0.833325	5.69E-56	-1.69E-46
MS.gene053080	MS.gene28864	0.830986	2.16E-55	-1.69E-46
MS.gene053226	MS.gene28864	0.81089	9.20E-51	-1.69E-46
MS.gene05364	MS.gene28864	0.820795	5.68E-53	-1.69E-46
MS.gene053931	MS.gene28864	0.817329	3.49E-52	-1.69E-46
MS.gene054657	MS.gene28864	0.841021	6.12E-58	-1.69E-46
MS.gene054994	MS.gene28864	0.809966	1.46E-50	-1.69E-46
MS.gene05510	MS.gene28864	0.806606	7.58E-50	-1.69E-46
MS.gene05517	MS.gene28864	0.820616	6.24E-53	-1.69E-46
MS.gene055512	MS.gene28864	0.831537	1.58E-55	-1.69E-46
MS.gene055623	MS.gene28864	0.84557	3.74E-59	-1.69E-46
MS.gene05604	MS.gene28864	0.803516	3.36E-49	-1.69E-46
MS.gene056181	MS.gene28864	0.807124	5.89E-50	-1.69E-46
MS.gene056506	MS.gene28864	0.808381	3.18E-50	-1.69E-46
MS.gene056760	MS.gene28864	0.81652	5.30E-52	-1.69E-46
MS.gene057060	MS.gene28864	0.829864	4.05E-55	-1.69E-46
MS.gene057140	MS.gene28864	0.828286	9.78E-55	-1.69E-46
MS.gene057208	MS.gene28864	0.810526	1.10E-50	-1.69E-46
MS.gene057392	MS.gene28864	0.810653	1.04E-50	-1.69E-46
MS.gene057693	MS.gene28864	0.843464	1.38E-58	-1.69E-46
MS.gene057705	MS.gene28864	0.805452	1.33E-49	-1.69E-46
MS.gene05795	MS.gene28864	0.80516	1.53E-49	-1.69E-46
MS.gene058399	MS.gene28864	0.800607	1.33E-48	-1.69E-46
MS.gene058548	MS.gene28864	0.815283	1.00E-51	-1.69E-46
MS.gene058595	MS.gene28864	0.804019	2.64E-49	-1.69E-46
MS.gene058625	MS.gene28864	0.801104	1.05E-48	-1.69E-46
MS.gene059317	MS.gene28864	0.824044	9.99E-54	-1.69E-46
MS.gene059569	MS.gene28864	0.818029	2.43E-52	-1.69E-46
MS.gene05979	MS.gene28864	0.815333	9.76E-52	-1.69E-46
MS.gene059830	MS.gene28864	0.818746	1.67E-52	-1.69E-46
MS.gene059842	MS.gene28864	0.831256	1.85E-55	-1.69E-46
MS.gene060310	MS.gene28864	0.817262	3.61E-52	-1.69E-46
MS.gene060322	MS.gene28864	0.833128	6.37E-56	-1.69E-46
MS.gene060586	MS.gene28864	0.805256	1.46E-49	-1.69E-46
MS.gene060803	MS.gene28864	0.838992	2.07E-57	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28864.t1	MTR_8g075290	98.780	82	1	0	1	82	7	88	3.89e-56	167

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28864.t1	AT4G35980	65.854	82	27	1	1	82	7	87	2.23e-33	110

Find 22 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCCAACTTCTCTAATTAAT+AGG	0.220533	8.2:+29783314	MS.gene28864:CDS
GTTTCAGCTAAATTATAAAA+AGG	0.298464	8.2:+29783002	None:intergenic
TCCCTATTAATTAGAGAAGT+TGG	0.353531	8.2:-29783316	None:intergenic
GCCAACTTCTCTAATTAATA+GGG	0.360365	8.2:+29783315	MS.gene28864:CDS
AAAATTTGTGATTGCAAGGA+TGG	0.400175	8.2:+29783056	MS.gene28864:CDS
TGCAGCATCTCCCGAGGCAA+TGG	0.407315	8.2:+29783351	MS.gene28864:CDS
GGATGACTCGCCCATTGCCT+CGG	0.414680	8.2:-29783362	None:intergenic
TAATCTTGCCGTTCCTGAGC+TGG	0.441007	8.2:-29783383	None:intergenic
GCAGCATCTCCCGAGGCAAT+GGG	0.454758	8.2:+29783352	MS.gene28864:CDS
GATGACTCGCCCATTGCCTC+GGG	0.464021	8.2:-29783361	None:intergenic
TTTGAGGTTACATTTGATGA+AGG	0.482661	8.2:+29783101	MS.gene28864:CDS
ATTCAAAATTTGTGATTGCA+AGG	0.486628	8.2:+29783052	MS.gene28864:CDS
TGATTGCAAGGATGGGAAGT+AGG	0.501741	8.2:+29783064	MS.gene28864:CDS
GGACTCGGCCATGTGATTTG+AGG	0.508194	8.2:+29783085	MS.gene28864:CDS
CAAGGATGGGAAGTAGGACT+CGG	0.540501	8.2:+29783070	MS.gene28864:CDS
AAATTTGTGATTGCAAGGAT+GGG	0.573450	8.2:+29783057	MS.gene28864:CDS
ATGGGCGAGTCATCCAGCTC+AGG	0.577264	8.2:+29783370	MS.gene28864:CDS
TGAATAATTGTACAACGTGT+CGG	0.581731	8.2:-29783034	None:intergenic
CGAGTCATCCAGCTCAGGAA+CGG	0.587000	8.2:+29783375	MS.gene28864:CDS
TCGGGAGATGCTGCAGCGTG+TGG	0.595356	8.2:-29783343	None:intergenic
ACACGCTGCAGCATCTCCCG+AGG	0.615860	8.2:+29783345	MS.gene28864:CDS
AAATGTAACCTCAAATCACA+TGG	0.661853	8.2:-29783093	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	CTGTTTTTTTGTTTTTTTTT+TGG	+	chr8.2:29783164-29783183	MS.gene28864:intron	15.0%
!!!	TATCATATTATGTTTCTATA+TGG	+	chr8.2:29783188-29783207	MS.gene28864:intron	15.0%
!!!	AATTCTGTTATTTTCTCATT+AGG	+	chr8.2:29783134-29783153	MS.gene28864:intron	20.0%
!	ATTCAAAATTTGTGATTGCA+AGG	+	chr8.2:29783052-29783071	MS.gene28864:CDS	25.0%
!!!	TAGGTGTTATAATCATTTTC+AGG	+	chr8.2:29783279-29783298	MS.gene28864:intron	25.0%
	AAAATTTGTGATTGCAAGGA+TGG	+	chr8.2:29783056-29783075	MS.gene28864:CDS	30.0%
	AAATGTAACCTCAAATCACA+TGG	-	chr8.2:29783096-29783115	None:intergenic	30.0%
	AAATTTGTGATTGCAAGGAT+GGG	+	chr8.2:29783057-29783076	MS.gene28864:CDS	30.0%
	ATGATTATAACACCTATGTC+AGG	-	chr8.2:29783275-29783294	None:intergenic	30.0%
	GCCAACTTCTCTAATTAATA+GGG	+	chr8.2:29783315-29783334	MS.gene28864:CDS	30.0%
	GTTTCTATATGGAAGATGAA+GGG	+	chr8.2:29783199-29783218	MS.gene28864:intron	30.0%
	TCCCTATTAATTAGAGAAGT+TGG	-	chr8.2:29783319-29783338	None:intergenic	30.0%
	TGAATAATTGTACAACGTGT+CGG	-	chr8.2:29783037-29783056	None:intergenic	30.0%
	TGCCAACTTCTCTAATTAAT+AGG	+	chr8.2:29783314-29783333	MS.gene28864:CDS	30.0%
!	TGTTTCTATATGGAAGATGA+AGG	+	chr8.2:29783198-29783217	MS.gene28864:intron	30.0%
!	TTTGAGGTTACATTTGATGA+AGG	+	chr8.2:29783101-29783120	MS.gene28864:CDS	30.0%
!!	AATCATTTTCAGGAGATTTC+AGG	+	chr8.2:29783289-29783308	MS.gene28864:intron	30.0%
	CCATCTCTCTAACCTGACAT+AGG	+	chr8.2:29783260-29783279	MS.gene28864:intron	45.0%
	CCTATGTCAGGTTAGAGAGA+TGG	-	chr8.2:29783263-29783282	None:intergenic	45.0%
	TGATTGCAAGGATGGGAAGT+AGG	+	chr8.2:29783064-29783083	MS.gene28864:CDS	45.0%
	CAAGGATGGGAAGTAGGACT+CGG	+	chr8.2:29783070-29783089	MS.gene28864:CDS	50.0%
	TAATCTTGCCGTTCCTGAGC+TGG	-	chr8.2:29783386-29783405	None:intergenic	50.0%
	CGAGTCATCCAGCTCAGGAA+CGG	+	chr8.2:29783375-29783394	MS.gene28864:CDS	55.0%
	GGACTCGGCCATGTGATTTG+AGG	+	chr8.2:29783085-29783104	MS.gene28864:CDS	55.0%
	ATGGGCGAGTCATCCAGCTC+AGG	+	chr8.2:29783370-29783389	MS.gene28864:CDS	60.0%
	GATGACTCGCCCATTGCCTC+GGG	-	chr8.2:29783364-29783383	None:intergenic	60.0%
	GCAGCATCTCCCGAGGCAAT+GGG	+	chr8.2:29783352-29783371	MS.gene28864:CDS	60.0%
	GGATGACTCGCCCATTGCCT+CGG	-	chr8.2:29783365-29783384	None:intergenic	60.0%
	TGCAGCATCTCCCGAGGCAA+TGG	+	chr8.2:29783351-29783370	MS.gene28864:CDS	60.0%
	ACACGCTGCAGCATCTCCCG+AGG	+	chr8.2:29783345-29783364	MS.gene28864:CDS	65.0%
	TCGGGAGATGCTGCAGCGTG+TGG	-	chr8.2:29783346-29783365	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	29783009	29783435	29783009	ID=MS.gene28864
chr8.2	mRNA	29783009	29783435	29783009	ID=MS.gene28864.t1;Parent=MS.gene28864
chr8.2	exon	29783009	29783122	29783009	ID=MS.gene28864.t1.exon1;Parent=MS.gene28864.t1
chr8.2	CDS	29783009	29783122	29783009	ID=cds.MS.gene28864.t1;Parent=MS.gene28864.t1
chr8.2	exon	29783301	29783435	29783301	ID=MS.gene28864.t1.exon2;Parent=MS.gene28864.t1
chr8.2	CDS	29783301	29783435	29783301	ID=cds.MS.gene28864.t1;Parent=MS.gene28864.t1

Gene Sequence

>MS.gene28864

CTAAATTATAAAAAGGAAGCTGCTGCCGACACGTTGTACAATTATTCAAAATTTGTGATTGCAAGGATGGGAAGTAGGACTCGGCCATGTGATTTGAGGTTACATTTGATGAAGGTTTGAGACAAAATTCTGTTATTTTCTCATTAGGAACAGCACTGTTTTTTTGTTTTTTTTTTGGTTATCATATTATGTTTCTATATGGAAGATGAAGGGATTATCTTGAAAACTAGTTTGTTAATATTCTTATTGATCCATCTCTCTAACCTGACATAGGTGTTATAATCATTTTCAGGAGATTTCAGGCATGCCAACTTCTCTAATTAATAGGGAAACACCACACGCTGCAGCATCTCCCGAGGCAATGGGCGAGTCATCCAGCTCAGGAACGGCAAGATTAGATAAAGCAGACAGTTTTCGAGCAATATAG

Protein sequence

>MS.gene28864.t1

LNYKKEAAADTLYNYSKFVIARMGSRTRPCDLRLHLMKEISGMPTSLINRETPHAAASPEAMGESSSSGTARLDKADSFRAI