AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31540.t1 MTR_2g089765 93.023 473 33 0 1 473 16 488 0.0 919
MS.gene31540.t1 MTR_2g089670 72.479 476 114 6 1 473 22 483 0.0 663
MS.gene31540.t1 MTR_8g012750 69.710 482 127 11 1 471 3 476 0.0 627
MS.gene31540.t1 MTR_2g089665 60.135 444 65 6 1 442 2 335 4.43e-173 490
MS.gene31540.t1 MTR_7g015860 42.946 482 234 12 1 467 5 460 3.81e-119 357
MS.gene31540.t1 MTR_3g028210 35.892 482 275 10 1 470 8 467 1.88e-97 301
MS.gene31540.t1 MTR_2g089650 36.100 482 272 13 1 470 9 466 1.68e-88 278
MS.gene31540.t1 MTR_7g014330 38.235 476 254 14 2 463 3 452 2.08e-88 278
MS.gene31540.t1 MTR_2g089755 36.009 461 262 12 24 470 37 478 3.08e-83 265
MS.gene31540.t1 MTR_6g015850 36.100 482 265 14 2 470 9 460 3.15e-83 264
MS.gene31540.t1 MTR_7g014230 37.076 472 265 12 2 463 5 454 8.51e-82 261
MS.gene31540.t1 MTR_6g015815 35.135 481 274 12 2 470 9 463 1.71e-81 260
MS.gene31540.t1 MTR_7g014250 36.229 472 269 12 2 463 3 452 6.22e-81 258
MS.gene31540.t1 MTR_2g089735 35.590 458 257 14 28 470 30 464 2.45e-78 252
MS.gene31540.t1 MTR_3g087640 35.113 487 273 15 2 468 10 473 1.06e-77 250
MS.gene31540.t1 MTR_6g015830 35.124 484 272 13 1 470 8 463 2.16e-77 249
MS.gene31540.t1 MTR_7g014200 34.490 490 272 15 1 469 9 470 1.34e-76 248
MS.gene31540.t1 MTR_6g015320 32.278 474 285 11 6 470 11 457 2.45e-71 233
MS.gene31540.t1 MTR_7g014160 33.811 488 279 15 1 467 9 473 2.92e-71 234
MS.gene31540.t1 MTR_7g014610 33.533 501 273 17 1 472 8 477 3.47e-69 228
MS.gene31540.t1 MTR_6g015315 32.589 448 275 7 28 470 30 455 8.15e-69 227
MS.gene31540.t1 MTR_7g013850 31.501 473 272 13 25 467 30 480 1.42e-62 211
MS.gene31540.t1 MTR_7g014620 33.261 463 253 12 20 467 11 432 2.56e-58 198
MS.gene31540.t1 MTR_7g014360 30.673 401 233 11 69 463 21 382 2.41e-46 165
MS.gene31540.t1 MTR_3g435300 35.816 282 157 8 2 273 4 271 1.95e-43 154
MS.gene31540.t1 MTR_2g089745 89.157 83 9 0 391 473 1 83 6.06e-42 144
MS.gene31540.t1 MTR_7g014630 34.256 289 153 10 1 270 9 279 2.45e-32 127
MS.gene31540.t1 MTR_7g014310 29.942 344 172 10 108 449 29 305 1.36e-31 123
MS.gene31540.t1 MTR_6g015310 27.604 384 204 10 71 445 15 333 4.18e-31 122
MS.gene31540.t1 MTR_7g014670 37.037 189 110 2 280 467 79 259 1.50e-30 119
MS.gene31540.t1 MTR_7g014180 38.202 178 96 5 1 174 7 174 4.42e-26 104
MS.gene31540.t1 MTR_8g041770 26.341 410 239 12 24 419 30 390 2.30e-19 90.5
MS.gene31540.t1 MTR_4g094835 24.481 482 279 17 1 452 4 430 2.36e-18 87.8
MS.gene31540.t1 MTR_3g098980 24.331 411 254 11 59 459 65 428 2.37e-18 87.4
MS.gene31540.t1 MTR_7g026860 24.619 394 243 13 38 419 38 389 1.06e-17 85.5
MS.gene31540.t1 MTR_4g022825 25.060 415 237 15 30 419 26 391 1.12e-16 82.4
MS.gene31540.t1 MTR_7g014210 39.130 115 68 2 325 437 24 138 1.52e-16 77.4
MS.gene31540.t1 MTR_8g096530 24.779 452 268 18 34 471 36 429 1.76e-16 82.0
MS.gene31540.t1 MTR_4g022720 24.574 411 247 13 24 419 24 386 2.53e-16 81.3
MS.gene31540.t1 MTR_3g088420 24.764 424 248 13 13 419 15 384 5.49e-16 80.5
MS.gene31540.t1 MTR_4g022670 23.284 408 262 13 24 419 24 392 1.43e-15 79.0
MS.gene31540.t1 MTR_4g022800 23.866 419 259 15 18 419 18 393 2.29e-15 78.6
MS.gene31540.t1 MTR_3g070170 21.915 470 294 16 22 459 31 459 3.11e-15 78.2
MS.gene31540.t1 MTR_0190s0020 25.296 423 257 13 45 452 48 426 4.69e-15 77.4
MS.gene31540.t1 MTR_4g127650 23.909 481 278 15 22 467 29 456 6.47e-15 77.0
MS.gene31540.t1 MTR_7g014340 57.353 68 28 1 2 68 3 70 8.03e-15 70.5
MS.gene31540.t1 MTR_3g026310 23.529 425 267 14 13 419 15 399 5.62e-14 74.3
MS.gene31540.t1 MTR_2g064495 21.918 438 285 14 40 465 39 431 6.54e-14 73.9
MS.gene31540.t1 MTR_7g014220 57.143 63 27 0 16 78 10 72 6.78e-14 67.4
MS.gene31540.t1 MTR_7g014205 37.719 114 59 2 323 435 18 120 8.40e-14 68.2
MS.gene31540.t1 MTR_4g094828 21.513 423 260 15 20 419 25 398 9.42e-14 73.6
MS.gene31540.t1 MTR_8g075610 24.306 432 267 17 40 459 30 413 1.65e-13 72.8
MS.gene31540.t1 MTR_3g435290 33.884 121 77 2 348 468 1 118 2.13e-13 67.0
MS.gene31540.t1 MTR_3g049150 26.059 307 167 11 140 419 126 399 2.14e-11 66.2
MS.gene31540.t1 MTR_3g101960 22.727 484 293 20 1 456 6 436 2.77e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31540.t1 AT1G03940 39.167 480 264 12 2 472 9 469 1.28e-104 320
MS.gene31540.t1 AT1G03495 40.169 356 181 12 3 349 10 342 1.78e-70 228
MS.gene31540.t1 AT3G29670 31.950 482 278 15 1 467 3 449 5.09e-65 217
MS.gene31540.t1 AT5G61160 31.315 479 290 14 1 469 3 452 1.68e-62 210
MS.gene31540.t1 AT3G29635 30.658 486 290 12 1 470 3 457 1.50e-60 205
MS.gene31540.t1 AT3G29590 32.427 478 277 16 1 468 8 449 6.90e-58 198
MS.gene31540.t1 AT3G29680 30.042 476 295 10 1 468 3 448 2.89e-56 194
MS.gene31540.t1 AT5G39090 30.105 475 292 13 1 467 5 447 1.04e-55 192
MS.gene31540.t1 AT5G39050 29.796 490 293 13 1 469 10 469 4.43e-53 186
MS.gene31540.t1 AT5G39080 27.778 486 303 15 1 467 5 461 4.12e-49 175
MS.gene31540.t1 AT3G48720 22.936 436 271 12 37 459 41 424 3.90e-18 87.0
MS.gene31540.t1 AT3G29690 31.481 162 99 4 312 469 27 180 1.77e-16 77.8
MS.gene31540.t1 AT5G41040 23.598 428 267 14 54 470 63 441 1.25e-15 79.3
MS.gene31540.t1 AT5G41040 23.598 428 267 14 54 470 79 457 1.41e-15 79.3
MS.gene31540.t1 AT1G65450 24.776 335 207 13 144 467 149 449 6.16e-13 70.9
MS.gene31540.t1 AT5G01210 22.674 344 216 10 144 461 141 460 1.08e-11 67.4

Find 100 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATCATTTCATGAAATTC+TGG 0.154366 2.4:+11281255 MS.gene31540:CDS
GGAGGGGTGTTAAGGGAATT+TGG 0.209569 2.4:-11280978 None:intergenic
GCTGTTTATGAGACTGATTT+TGG 0.237069 2.4:+11281956 MS.gene31540:CDS
AAACATTGATGTCTGATTAT+AGG 0.245779 2.4:+11281879 MS.gene31540:CDS
GGTTGCTGGTTCACCGAAAT+TGG 0.246480 2.4:+11281934 MS.gene31540:CDS
GTACGAGGTGGAGGTAAATT+TGG 0.264562 2.4:-11281116 None:intergenic
CATCCCTATAATGGCTATTC+AGG 0.277699 2.4:+11281160 MS.gene31540:CDS
TAACCCATTTCTTCAACTTC+TGG 0.280416 2.4:-11281514 None:intergenic
AACATTGATGTCTGATTATA+GGG 0.290004 2.4:+11281880 MS.gene31540:CDS
GTTGGTGATGGAACTGAATT+TGG 0.302858 2.4:-11280771 None:intergenic
GTAAAGAGAGATGAGTTAAT+TGG 0.307415 2.4:+11281776 MS.gene31540:CDS
CAAATAGACAACCCAGAGTT+TGG 0.316268 2.4:-11281194 None:intergenic
TTTAAGAGTGATGCTTTGTT+AGG 0.320839 2.4:+11281851 MS.gene31540:CDS
CTTTAACTCTATCTCTTTCA+TGG 0.321227 2.4:-11281355 None:intergenic
GTGACCTGAATAGCCATTAT+AGG 0.322860 2.4:-11281164 None:intergenic
GTCACTGTTCTTCCAAACTC+TGG 0.327811 2.4:+11281182 MS.gene31540:CDS
TGACATGGAATTCGCGGGTT+TGG 0.346535 2.4:+11281433 MS.gene31540:CDS
GTATATAAGGAGGGGTGTTA+AGG 0.354241 2.4:-11280986 None:intergenic
ACTATGTTCTATCACCTTCA+TGG 0.354613 2.4:-11280733 None:intergenic
ATAATGAGGTTGGAGGAAAA+AGG 0.355937 2.4:-11280951 None:intergenic
TTGTAACAAGCTCCTTGATT+TGG 0.359633 2.4:+11281585 MS.gene31540:CDS
TAGAAGAAGATACGTTTAAC+AGG 0.362847 2.4:-11280840 None:intergenic
TGAACTGCATCAGATTTCTT+AGG 0.374068 2.4:-11281989 None:intergenic
GAAGAAGTGTTGGAGGGTTA+TGG 0.375546 2.4:-11280928 None:intergenic
AAGTCCAGAAGTTGAAGAAA+TGG 0.376086 2.4:+11281510 MS.gene31540:CDS
AAGTCTGTTACTGTGGTTGC+TGG 0.378057 2.4:+11281920 MS.gene31540:CDS
TCATCATTTCATGAAATTCT+GGG 0.381535 2.4:+11281256 MS.gene31540:CDS
CATTCGTGTCATCCCTATAA+TGG 0.385956 2.4:+11281151 MS.gene31540:CDS
GTCTCATAAACAGCCAATTT+CGG 0.394758 2.4:-11281947 None:intergenic
GGAAAGTGAGTGTTTAAGAA+TGG 0.400352 2.4:-11280907 None:intergenic
ATTATTGTTGTTTGTTGCTA+TGG 0.405288 2.4:-11281294 None:intergenic
ATTCACAAGATGCTCAAAAC+TGG 0.405633 2.4:+11281081 MS.gene31540:CDS
TGACCTGAATAGCCATTATA+GGG 0.424676 2.4:-11281163 None:intergenic
GGGAATTTGGAGGGATAATG+AGG 0.435908 2.4:-11280965 None:intergenic
TCATCAAGGTAACGTATATA+AGG 0.436544 2.4:-11280999 None:intergenic
TATATAAGGAGGGGTGTTAA+GGG 0.438164 2.4:-11280985 None:intergenic
TCTCTTTCTGATTGTAGAGA+TGG 0.439448 2.4:+11282034 MS.gene31540:CDS
AGATAGAGTTAAAGATCTAA+AGG 0.440490 2.4:+11281364 MS.gene31540:CDS
TGGTATGTCGAAGAAAGTTA+AGG 0.445120 2.4:-11280802 None:intergenic
AGGTGGAATTGAAGTTGGTT+TGG 0.449191 2.4:+11282060 MS.gene31540:CDS
CTTTCTGATTGTAGAGATGG+TGG 0.471103 2.4:+11282037 MS.gene31540:CDS
GGGATTGTTGTGGCTTCAAT+TGG 0.477890 2.4:+11281806 MS.gene31540:CDS
GGTGGAGGTGGAATTGAAGT+TGG 0.477895 2.4:+11282055 MS.gene31540:CDS
GATAGAGTTAAAGATCTAAA+GGG 0.478327 2.4:+11281365 MS.gene31540:CDS
TCACTGTTCTTCCAAACTCT+GGG 0.482664 2.4:+11281183 MS.gene31540:CDS
GAAAGTGAGTGTTTAAGAAT+GGG 0.483955 2.4:-11280906 None:intergenic
GGTATGTCGAAGAAAGTTAA+GGG 0.489263 2.4:-11280801 None:intergenic
GGTGATGGAACTGAATTTGG+TGG 0.489909 2.4:-11280768 None:intergenic
GCAGTTCATCTTGATTCGTC+CGG 0.491791 2.4:+11282004 MS.gene31540:CDS
AGTCCAGAAGTTGAAGAAAT+GGG 0.492194 2.4:+11281511 MS.gene31540:CDS
ATTTGGAGGGATAATGAGGT+TGG 0.499099 2.4:-11280961 None:intergenic
TGGAGGGATAATGAGGTTGG+AGG 0.502132 2.4:-11280958 None:intergenic
TTTAACTCTATCTCTTTCAT+GGG 0.506937 2.4:-11281354 None:intergenic
AAGGGGAGAATGGTTGGTGA+TGG 0.521926 2.4:-11280783 None:intergenic
AAGTTGGTTTGGCTCTTGAG+AGG 0.523983 2.4:+11282071 MS.gene31540:CDS
GTTTGAAGAAAGTGATGAGT+TGG 0.526798 2.4:-11280876 None:intergenic
GATCATACAAAACCAAATCA+AGG 0.527811 2.4:-11281597 None:intergenic
ATCAAATGACATGGAATTCG+CGG 0.527943 2.4:+11281427 MS.gene31540:CDS
GATTGTAGAGATGGTGGAGG+TGG 0.541712 2.4:+11282043 MS.gene31540:CDS
ACTCTATCTCTTTCATGGGA+AGG 0.547296 2.4:-11281350 None:intergenic
AAAGTTAAGGGGAGAATGGT+TGG 0.550742 2.4:-11280789 None:intergenic
TTGTCTTGCATCTTATATTG+TGG 0.552776 2.4:+11281751 MS.gene31540:CDS
TCTTGTTCAGTACGAGGTGG+AGG 0.553828 2.4:-11281125 None:intergenic
TCAAATGACATGGAATTCGC+GGG 0.559911 2.4:+11281428 MS.gene31540:CDS
ATCAGAAAGAGAAATCGAAC+CGG 0.560110 2.4:-11282023 None:intergenic
GAAGAAAGTTAAGGGGAGAA+TGG 0.561998 2.4:-11280793 None:intergenic
GATGAGTTGGATGAGGAAAG+TGG 0.563879 2.4:-11280863 None:intergenic
CAAGGTAACGTATATAAGGA+GGG 0.577627 2.4:-11280995 None:intergenic
TGAGGATTCAGCGACGGTGA+AGG 0.579651 2.4:-11281033 None:intergenic
GGGGTGTTAAGGGAATTTGG+AGG 0.579772 2.4:-11280975 None:intergenic
CATGGGAAGGAAGATCAAGA+GGG 0.580759 2.4:-11281337 None:intergenic
GATGGAACTGAATTTGGTGG+TGG 0.582071 2.4:-11280765 None:intergenic
TACAACCATCTTGCTGCAGA+CGG 0.585188 2.4:+11281224 MS.gene31540:CDS
GAGGTAAATTTGGAACAAGA+TGG 0.586717 2.4:-11281106 None:intergenic
TAACTTTCTTCGACATACCA+TGG 0.593348 2.4:+11280805 MS.gene31540:CDS
ACCTGGTAAGTCTGTTACTG+TGG 0.593576 2.4:+11281913 MS.gene31540:CDS
TCAAGGTAACGTATATAAGG+AGG 0.593641 2.4:-11280996 None:intergenic
CTTGCAGATTGTCGTGATAG+CGG 0.594957 2.4:+11281695 MS.gene31540:CDS
TTGTATGATCAAATCAGAGG+AGG 0.597606 2.4:+11281610 MS.gene31540:CDS
GGGTGTTAAGGGAATTTGGA+GGG 0.614180 2.4:-11280974 None:intergenic
TGTATGATCAAATCAGAGGA+GGG 0.615042 2.4:+11281611 MS.gene31540:CDS
AAAAGGGAAGAAGTGTTGGA+GGG 0.615109 2.4:-11280934 None:intergenic
GTCTGCTGAGGATTCAGCGA+CGG 0.615979 2.4:-11281039 None:intergenic
TCTGATTGTAGAGATGGTGG+AGG 0.625713 2.4:+11282040 MS.gene31540:CDS
TATAGGGAAATATCAAAACC+TGG 0.626368 2.4:+11281896 MS.gene31540:CDS
ACCACAGTAACAGACTTACC+AGG 0.630856 2.4:-11281914 None:intergenic
TCATGGGAAGGAAGATCAAG+AGG 0.635606 2.4:-11281338 None:intergenic
AGATTCTGAATCAAATGACA+TGG 0.642714 2.4:+11281418 MS.gene31540:CDS
AGAAAGTGATGAGTTGGATG+AGG 0.649026 2.4:-11280870 None:intergenic
TTGTCTTGTTCAGTACGAGG+TGG 0.652618 2.4:-11281128 None:intergenic
AGGATAGAGAGTCTTCATCA+AGG 0.656629 2.4:-11281013 None:intergenic
GTATGTCGAAGAAAGTTAAG+GGG 0.670105 2.4:-11280800 None:intergenic
GAGGATGTTGAAGTCTGCTG+AGG 0.672509 2.4:-11281051 None:intergenic
AAGGTAACGTATATAAGGAG+GGG 0.677863 2.4:-11280994 None:intergenic
CATCTTGTGAATCAGATACG+AGG 0.681815 2.4:-11281070 None:intergenic
ATGTTGTCTTGTTCAGTACG+AGG 0.683962 2.4:-11281131 None:intergenic
ACAGGGTGACAATAAAACCA+TGG 0.687760 2.4:-11280822 None:intergenic
AGAAGAAGATACGTTTAACA+GGG 0.696018 2.4:-11280839 None:intergenic
TGGGAAGGAAGATCAAGAGG+GGG 0.698434 2.4:-11281335 None:intergenic
ATGGGAAGGAAGATCAAGAG+GGG 0.712513 2.4:-11281336 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAGTTAATTGGAAAAAAT+GGG + chr2.4:11281786-11281805 MS.gene31540:CDS 20.0%
!!! CAATTTCCAAAATAAGTTTT+TGG - chr2.4:11281734-11281753 None:intergenic 20.0%
!!! TCATTACCAAAAACTTATTT+TGG + chr2.4:11281725-11281744 MS.gene31540:CDS 20.0%
! AAACATTGATGTCTGATTAT+AGG + chr2.4:11281879-11281898 MS.gene31540:CDS 25.0%
! AACATTGATGTCTGATTATA+GGG + chr2.4:11281880-11281899 MS.gene31540:CDS 25.0%
! AGATAGAGTTAAAGATCTAA+AGG + chr2.4:11281364-11281383 MS.gene31540:CDS 25.0%
! GATAGAGTTAAAGATCTAAA+GGG + chr2.4:11281365-11281384 MS.gene31540:CDS 25.0%
! GATGAGTTAATTGGAAAAAA+TGG + chr2.4:11281785-11281804 MS.gene31540:CDS 25.0%
! TCATCATTTCATGAAATTCT+GGG + chr2.4:11281256-11281275 MS.gene31540:CDS 25.0%
! TTCATCATTTCATGAAATTC+TGG + chr2.4:11281255-11281274 MS.gene31540:CDS 25.0%
! TTTAACTCTATCTCTTTCAT+GGG - chr2.4:11281357-11281376 None:intergenic 25.0%
!!! ATTATTGTTGTTTGTTGCTA+TGG - chr2.4:11281297-11281316 None:intergenic 25.0%
AGAAGAAGATACGTTTAACA+GGG - chr2.4:11280842-11280861 None:intergenic 30.0%
AGATTCTGAATCAAATGACA+TGG + chr2.4:11281418-11281437 MS.gene31540:CDS 30.0%
CTTTAACTCTATCTCTTTCA+TGG - chr2.4:11281358-11281377 None:intergenic 30.0%
GAAAGTGAGTGTTTAAGAAT+GGG - chr2.4:11280909-11280928 None:intergenic 30.0%
GATCATACAAAACCAAATCA+AGG - chr2.4:11281600-11281619 None:intergenic 30.0%
GGTGATAAAAAAAGTGATCA+AGG + chr2.4:11281632-11281651 MS.gene31540:CDS 30.0%
GTAAAGAGAGATGAGTTAAT+TGG + chr2.4:11281776-11281795 MS.gene31540:CDS 30.0%
TAGAAGAAGATACGTTTAAC+AGG - chr2.4:11280843-11280862 None:intergenic 30.0%
TATAGGGAAATATCAAAACC+TGG + chr2.4:11281896-11281915 MS.gene31540:CDS 30.0%
TCATCAAGGTAACGTATATA+AGG - chr2.4:11281002-11281021 None:intergenic 30.0%
TTGTCTTGCATCTTATATTG+TGG + chr2.4:11281751-11281770 MS.gene31540:CDS 30.0%
! GTTTTGTATGATCAAATCAG+AGG + chr2.4:11281607-11281626 MS.gene31540:CDS 30.0%
! TTTAAGAGTGATGCTTTGTT+AGG + chr2.4:11281851-11281870 MS.gene31540:CDS 30.0%
!! CTGTTTATGAGACTGATTTT+GGG + chr2.4:11281957-11281976 MS.gene31540:CDS 30.0%
AAGGTAACGTATATAAGGAG+GGG - chr2.4:11280997-11281016 None:intergenic 35.0%
AAGTCCAGAAGTTGAAGAAA+TGG + chr2.4:11281510-11281529 MS.gene31540:CDS 35.0%
AGTCCAGAAGTTGAAGAAAT+GGG + chr2.4:11281511-11281530 MS.gene31540:CDS 35.0%
ATAATGAGGTTGGAGGAAAA+AGG - chr2.4:11280954-11280973 None:intergenic 35.0%
ATCAAATGACATGGAATTCG+CGG + chr2.4:11281427-11281446 MS.gene31540:CDS 35.0%
ATCAGAAAGAGAAATCGAAC+CGG - chr2.4:11282026-11282045 None:intergenic 35.0%
ATTCACAAGATGCTCAAAAC+TGG + chr2.4:11281081-11281100 MS.gene31540:CDS 35.0%
CAAGGTAACGTATATAAGGA+GGG - chr2.4:11280998-11281017 None:intergenic 35.0%
GGAAAGTGAGTGTTTAAGAA+TGG - chr2.4:11280910-11280929 None:intergenic 35.0%
GGTATGTCGAAGAAAGTTAA+GGG - chr2.4:11280804-11280823 None:intergenic 35.0%
GTATGTCGAAGAAAGTTAAG+GGG - chr2.4:11280803-11280822 None:intergenic 35.0%
GTCTCATAAACAGCCAATTT+CGG - chr2.4:11281950-11281969 None:intergenic 35.0%
GTTTGAAGAAAGTGATGAGT+TGG - chr2.4:11280879-11280898 None:intergenic 35.0%
TAACCCATTTCTTCAACTTC+TGG - chr2.4:11281517-11281536 None:intergenic 35.0%
TAACTTTCTTCGACATACCA+TGG + chr2.4:11280805-11280824 MS.gene31540:CDS 35.0%
TAATGAGGTTGGAGGAAAAA+GGG - chr2.4:11280953-11280972 None:intergenic 35.0%
TCAAGGTAACGTATATAAGG+AGG - chr2.4:11280999-11281018 None:intergenic 35.0%
TCTCTTTCTGATTGTAGAGA+TGG + chr2.4:11282034-11282053 MS.gene31540:CDS 35.0%
TGAACTGCATCAGATTTCTT+AGG - chr2.4:11281992-11282011 None:intergenic 35.0%
TGACCTGAATAGCCATTATA+GGG - chr2.4:11281166-11281185 None:intergenic 35.0%
TGGAAAAAATGGGATTGTTG+TGG + chr2.4:11281796-11281815 MS.gene31540:CDS 35.0%
TGGTATGTCGAAGAAAGTTA+AGG - chr2.4:11280805-11280824 None:intergenic 35.0%
TGTATGATCAAATCAGAGGA+GGG + chr2.4:11281611-11281630 MS.gene31540:CDS 35.0%
TTGTAACAAGCTCCTTGATT+TGG + chr2.4:11281585-11281604 MS.gene31540:CDS 35.0%
TTGTATGATCAAATCAGAGG+AGG + chr2.4:11281610-11281629 MS.gene31540:CDS 35.0%
! GAGGTAAATTTGGAACAAGA+TGG - chr2.4:11281109-11281128 None:intergenic 35.0%
!! GCTGTTTATGAGACTGATTT+TGG + chr2.4:11281956-11281975 MS.gene31540:CDS 35.0%
!! TATATAAGGAGGGGTGTTAA+GGG - chr2.4:11280988-11281007 None:intergenic 35.0%
!! TTATGAGACTGATTTTGGGT+GGG + chr2.4:11281961-11281980 MS.gene31540:CDS 35.0%
!! TTTATGAGACTGATTTTGGG+TGG + chr2.4:11281960-11281979 MS.gene31540:CDS 35.0%
AAAGTTAAGGGGAGAATGGT+TGG - chr2.4:11280792-11280811 None:intergenic 40.0%
ACAGGGTGACAATAAAACCA+TGG - chr2.4:11280825-11280844 None:intergenic 40.0%
ACTCTATCTCTTTCATGGGA+AGG - chr2.4:11281353-11281372 None:intergenic 40.0%
AGGATAGAGAGTCTTCATCA+AGG - chr2.4:11281016-11281035 None:intergenic 40.0%
ATTTGGAGGGATAATGAGGT+TGG - chr2.4:11280964-11280983 None:intergenic 40.0%
CAAATAGACAACCCAGAGTT+TGG - chr2.4:11281197-11281216 None:intergenic 40.0%
CATCCCTATAATGGCTATTC+AGG + chr2.4:11281160-11281179 MS.gene31540:CDS 40.0%
CATCTTGTGAATCAGATACG+AGG - chr2.4:11281073-11281092 None:intergenic 40.0%
CATTCGTGTCATCCCTATAA+TGG + chr2.4:11281151-11281170 MS.gene31540:CDS 40.0%
CTTTCTGATTGTAGAGATGG+TGG + chr2.4:11282037-11282056 MS.gene31540:CDS 40.0%
GAAGAAAGTTAAGGGGAGAA+TGG - chr2.4:11280796-11280815 None:intergenic 40.0%
GTGACCTGAATAGCCATTAT+AGG - chr2.4:11281167-11281186 None:intergenic 40.0%
TCAAATGACATGGAATTCGC+GGG + chr2.4:11281428-11281447 MS.gene31540:CDS 40.0%
TCACTGTTCTTCCAAACTCT+GGG + chr2.4:11281183-11281202 MS.gene31540:CDS 40.0%
! AGAAAGTGATGAGTTGGATG+AGG - chr2.4:11280873-11280892 None:intergenic 40.0%
! AGGAAAAAGGGAAGAAGTGT+TGG - chr2.4:11280941-11280960 None:intergenic 40.0%
! AGGTGGAATTGAAGTTGGTT+TGG + chr2.4:11282060-11282079 MS.gene31540:CDS 40.0%
! ATGTTGTCTTGTTCAGTACG+AGG - chr2.4:11281134-11281153 None:intergenic 40.0%
!! AAAAAGGGAAGAAGTGTTGG+AGG - chr2.4:11280938-11280957 None:intergenic 40.0%
!! AAAAGGGAAGAAGTGTTGGA+GGG - chr2.4:11280937-11280956 None:intergenic 40.0%
!! GTATATAAGGAGGGGTGTTA+AGG - chr2.4:11280989-11281008 None:intergenic 40.0%
!! GTTGGTGATGGAACTGAATT+TGG - chr2.4:11280774-11280793 None:intergenic 40.0%
!! TATGAGACTGATTTTGGGTG+GGG + chr2.4:11281962-11281981 MS.gene31540:CDS 40.0%
!! TGCTATGGCTTTTGAAAGAG+AGG - chr2.4:11281282-11281301 None:intergenic 40.0%
AAGTCTGTTACTGTGGTTGC+TGG + chr2.4:11281920-11281939 MS.gene31540:CDS 45.0%
ACCACAGTAACAGACTTACC+AGG - chr2.4:11281917-11281936 None:intergenic 45.0%
ACCTGGTAAGTCTGTTACTG+TGG + chr2.4:11281913-11281932 MS.gene31540:CDS 45.0%
ATGGGAAGGAAGATCAAGAG+GGG - chr2.4:11281339-11281358 None:intergenic 45.0%
CATGGGAAGGAAGATCAAGA+GGG - chr2.4:11281340-11281359 None:intergenic 45.0%
CTTGCAGATTGTCGTGATAG+CGG + chr2.4:11281695-11281714 MS.gene31540:CDS 45.0%
GGGAATTTGGAGGGATAATG+AGG - chr2.4:11280968-11280987 None:intergenic 45.0%
GGGATTGTTGTGGCTTCAAT+TGG + chr2.4:11281806-11281825 MS.gene31540:CDS 45.0%
GGGTGTTAAGGGAATTTGGA+GGG - chr2.4:11280977-11280996 None:intergenic 45.0%
GTACGAGGTGGAGGTAAATT+TGG - chr2.4:11281119-11281138 None:intergenic 45.0%
GTCACTGTTCTTCCAAACTC+TGG + chr2.4:11281182-11281201 MS.gene31540:CDS 45.0%
TACAACCATCTTGCTGCAGA+CGG + chr2.4:11281224-11281243 MS.gene31540:CDS 45.0%
TCATGGGAAGGAAGATCAAG+AGG - chr2.4:11281341-11281360 None:intergenic 45.0%
! ATTTTCCGTCTGCAGCAAGA+TGG - chr2.4:11281232-11281251 None:intergenic 45.0%
! GATGAGTTGGATGAGGAAAG+TGG - chr2.4:11280866-11280885 None:intergenic 45.0%
! GATGGAACTGAATTTGGTGG+TGG - chr2.4:11280768-11280787 None:intergenic 45.0%
! GCAGTTCATCTTGATTCGTC+CGG + chr2.4:11282004-11282023 MS.gene31540:CDS 45.0%
! TCTGATTGTAGAGATGGTGG+AGG + chr2.4:11282040-11282059 MS.gene31540:CDS 45.0%
! TTGTCTTGTTCAGTACGAGG+TGG - chr2.4:11281131-11281150 None:intergenic 45.0%
!! AAGTTGGTTTGGCTCTTGAG+AGG + chr2.4:11282071-11282090 MS.gene31540:CDS 45.0%
!! ATGAGACTGATTTTGGGTGG+GGG + chr2.4:11281963-11281982 MS.gene31540:CDS 45.0%
!! GAAGAAGTGTTGGAGGGTTA+TGG - chr2.4:11280931-11280950 None:intergenic 45.0%
!! GGTGATGGAACTGAATTTGG+TGG - chr2.4:11280771-11280790 None:intergenic 45.0%
GAGGATGTTGAAGTCTGCTG+AGG - chr2.4:11281054-11281073 None:intergenic 50.0%
GGTGGAGGTGGAATTGAAGT+TGG + chr2.4:11282055-11282074 MS.gene31540:CDS 50.0%
TCTTGTTCAGTACGAGGTGG+AGG - chr2.4:11281128-11281147 None:intergenic 50.0%
TGACATGGAATTCGCGGGTT+TGG + chr2.4:11281433-11281452 MS.gene31540:CDS 50.0%
TGGAGGGATAATGAGGTTGG+AGG - chr2.4:11280961-11280980 None:intergenic 50.0%
TGGGAAGGAAGATCAAGAGG+GGG - chr2.4:11281338-11281357 None:intergenic 50.0%
! GGGGTGTTAAGGGAATTTGG+AGG - chr2.4:11280978-11280997 None:intergenic 50.0%
!! AAGGGGAGAATGGTTGGTGA+TGG - chr2.4:11280786-11280805 None:intergenic 50.0%
!! GATTGTAGAGATGGTGGAGG+TGG + chr2.4:11282043-11282062 MS.gene31540:CDS 50.0%
!! GGAGGGGTGTTAAGGGAATT+TGG - chr2.4:11280981-11281000 None:intergenic 50.0%
!! GGTTGCTGGTTCACCGAAAT+TGG + chr2.4:11281934-11281953 MS.gene31540:CDS 50.0%
GTCTGCTGAGGATTCAGCGA+CGG - chr2.4:11281042-11281061 None:intergenic 55.0%
TGAGGATTCAGCGACGGTGA+AGG - chr2.4:11281036-11281055 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 11280735 11282156 11280735 ID=MS.gene31540
chr2.4 mRNA 11280735 11282156 11280735 ID=MS.gene31540.t1;Parent=MS.gene31540
chr2.4 exon 11280735 11282156 11280735 ID=MS.gene31540.t1.exon1;Parent=MS.gene31540.t1
chr2.4 CDS 11280735 11282156 11280735 ID=cds.MS.gene31540.t1;Parent=MS.gene31540.t1
Gene Sequence

>MS.gene31540

ATGAAGGTGATAGAACATAGTCAAGTTGCACCACCACCAAATTCAGTTCCATCACCAACCATTCTCCCCTTAACTTTCTTCGACATACCATGGTTTTATTGTCACCCTGTTAAACGTATCTTCTTCTACCACTTTCCTCATCCAACTCATCACTTTCTTCAAACAACACTTCCCATTCTTAAACACTCACTTTCCATAACCCTCCAACACTTCTTCCCTTTTTCCTCCAACCTCATTATCCCTCCAAATTCCCTTAACACCCCTCCTTATATACGTTACCTTGATGAAGACTCTCTATCCTTCACCGTCGCTGAATCCTCAGCAGACTTCAACATCCTCGTATCTGATTCACAAGATGCTCAAAACTGGCACCATCTTGTTCCAAATTTACCTCCACCTCGTACTGAACAAGACAACATTCGTGTCATCCCTATAATGGCTATTCAGGTCACTGTTCTTCCAAACTCTGGGTTGTCTATTTGCTTAAGTTACAACCATCTTGCTGCAGACGGAAAATCACTTCATCATTTCATGAAATTCTGGGCCTCTCTTTCAAAAGCCATAGCAACAAACAACAATAATTTGTCTCTTGAATACTCTCCCCCTCTTGATCTTCCTTCCCATGAAAGAGATAGAGTTAAAGATCTAAAGGGTCTTATACTTGTATACTTGCAAGAATTAGAAGATTCTGAATCAAATGACATGGAATTCGCGGGTTTGGTTCAAGATTCTTATGTTAACAAGATAAGAACAACTTTAGTATTGAGCTATGAACAAGTCCAGAAGTTGAAGAAATGGGTTACTGATAAATGTAAAGAGAGTTATAGAATACAACACTTGTCAACTTTTGTTGTAACAAGCTCCTTGATTTGGTTTTGTATGATCAAATCAGAGGAGGGTGATAAAAAAAGTGATCAAGGTGATTGTGTTGATGTTGATGATCACTGCTATTTTGTGTTTCTTGCAGATTGTCGTGATAGCGGTGAATTTTCATTACCAAAAACTTATTTTGGAAATTGTCTTGCATCTTATATTGTGGCTGTAAAGAGAGATGAGTTAATTGGAAAAAATGGGATTGTTGTGGCTTCAATTGGTATTGAAAAGAAGATAAGAGATTTTAAGAGTGATGCTTTGTTAGGAGCTGAAACATTGATGTCTGATTATAGGGAAATATCAAAACCTGGTAAGTCTGTTACTGTGGTTGCTGGTTCACCGAAATTGGCTGTTTATGAGACTGATTTTGGGTGGGGGAAGCCTAAGAAATCTGATGCAGTTCATCTTGATTCGTCCGGTTCGATTTCTCTTTCTGATTGTAGAGATGGTGGAGGTGGAATTGAAGTTGGTTTGGCTCTTGAGAGGTCTAGAATGACTAATTTCATTAACATCTTTCAAGAACAACTTGATAATATTTGTAGCATGTGA
Protein sequence

>MS.gene31540.t1

MKVIEHSQVAPPPNSVPSPTILPLTFFDIPWFYCHPVKRIFFYHFPHPTHHFLQTTLPILKHSLSITLQHFFPFSSNLIIPPNSLNTPPYIRYLDEDSLSFTVAESSADFNILVSDSQDAQNWHHLVPNLPPPRTEQDNIRVIPIMAIQVTVLPNSGLSICLSYNHLAADGKSLHHFMKFWASLSKAIATNNNNLSLEYSPPLDLPSHERDRVKDLKGLILVYLQELEDSESNDMEFAGLVQDSYVNKIRTTLVLSYEQVQKLKKWVTDKCKESYRIQHLSTFVVTSSLIWFCMIKSEEGDKKSDQGDCVDVDDHCYFVFLADCRDSGEFSLPKTYFGNCLASYIVAVKRDELIGKNGIVVASIGIEKKIRDFKSDALLGAETLMSDYREISKPGKSVTVVAGSPKLAVYETDFGWGKPKKSDAVHLDSSGSISLSDCRDGGGGIEVGLALERSRMTNFINIFQEQLDNICSM