AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31546

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31546.t1 MTR_2g089670 81.328 482 82 2 5 486 10 483 0.0 779
MS.gene31546.t1 MTR_2g089765 75.051 493 104 8 2 486 7 488 0.0 737
MS.gene31546.t1 MTR_8g012750 65.083 484 147 13 15 484 1 476 0.0 588
MS.gene31546.t1 MTR_2g089665 63.946 441 52 4 16 455 1 335 0.0 532
MS.gene31546.t1 MTR_7g015860 44.330 485 224 12 17 482 5 462 3.22e-123 368
MS.gene31546.t1 MTR_3g028210 36.722 482 270 11 16 483 7 467 1.51e-93 291
MS.gene31546.t1 MTR_2g089650 36.515 482 267 14 17 483 9 466 3.04e-89 281
MS.gene31546.t1 MTR_7g014330 37.959 490 247 15 16 481 1 457 4.69e-89 280
MS.gene31546.t1 MTR_6g015850 36.478 477 269 10 17 483 8 460 1.63e-86 273
MS.gene31546.t1 MTR_6g015815 36.920 474 272 10 18 483 9 463 8.01e-86 271
MS.gene31546.t1 MTR_7g014230 37.238 478 253 13 18 476 5 454 7.88e-83 264
MS.gene31546.t1 MTR_2g089755 35.546 467 253 15 40 483 37 478 1.90e-81 261
MS.gene31546.t1 MTR_6g015830 35.430 477 277 11 17 483 8 463 3.53e-80 257
MS.gene31546.t1 MTR_7g014250 35.670 485 255 15 16 476 1 452 4.98e-79 254
MS.gene31546.t1 MTR_3g087640 35.052 485 271 15 18 480 10 472 1.52e-78 253
MS.gene31546.t1 MTR_7g014200 33.874 493 278 16 12 482 4 470 2.48e-77 250
MS.gene31546.t1 MTR_2g089735 34.607 445 257 11 54 483 39 464 3.88e-77 249
MS.gene31546.t1 MTR_7g014160 34.280 493 279 15 10 480 4 473 1.42e-74 243
MS.gene31546.t1 MTR_6g015320 33.475 469 283 10 22 483 11 457 5.56e-74 241
MS.gene31546.t1 MTR_6g015315 34.483 435 262 8 54 483 39 455 2.78e-70 231
MS.gene31546.t1 MTR_7g014610 33.468 496 283 15 9 480 3 475 7.73e-70 230
MS.gene31546.t1 MTR_7g013850 31.646 474 267 17 41 480 30 480 2.14e-61 208
MS.gene31546.t1 MTR_7g014620 31.957 460 260 12 36 480 11 432 2.21e-55 191
MS.gene31546.t1 MTR_7g014360 30.788 406 230 10 87 481 22 387 2.20e-51 179
MS.gene31546.t1 MTR_3g435300 36.111 288 153 11 18 294 4 271 8.47e-42 150
MS.gene31546.t1 MTR_6g015310 29.032 372 201 9 89 458 23 333 1.95e-33 129
MS.gene31546.t1 MTR_2g089745 75.309 81 20 0 406 486 3 83 7.08e-33 120
MS.gene31546.t1 MTR_7g014630 32.069 290 163 11 17 291 9 279 3.45e-31 124
MS.gene31546.t1 MTR_7g014670 35.602 191 120 2 291 480 71 259 3.67e-31 121
MS.gene31546.t1 MTR_7g014310 28.446 341 179 10 125 463 29 306 3.07e-30 120
MS.gene31546.t1 MTR_7g014180 37.989 179 96 6 17 191 7 174 5.05e-26 104
MS.gene31546.t1 MTR_8g041770 27.543 403 246 13 37 432 27 390 5.20e-23 101
MS.gene31546.t1 MTR_4g022670 26.056 426 268 17 17 432 4 392 3.07e-20 93.6
MS.gene31546.t1 MTR_4g022720 26.214 412 248 13 34 432 18 386 1.01e-19 91.7
MS.gene31546.t1 MTR_3g101900 23.195 457 288 16 40 474 30 445 1.79e-19 91.3
MS.gene31546.t1 MTR_7g026860 25.610 410 256 14 34 432 18 389 1.86e-18 88.2
MS.gene31546.t1 MTR_4g022800 25.369 406 266 13 34 432 18 393 3.07e-17 84.3
MS.gene31546.t1 MTR_3g088420 27.123 424 235 18 29 432 15 384 6.61e-17 83.2
MS.gene31546.t1 MTR_7g014210 39.130 115 68 2 338 450 24 138 1.46e-16 77.8
MS.gene31546.t1 MTR_6g045553 25.053 475 263 18 40 469 35 461 5.80e-16 80.5
MS.gene31546.t1 MTR_7g085930 23.952 501 307 17 16 483 5 464 1.80e-15 79.0
MS.gene31546.t1 MTR_3g101930 24.466 421 269 18 63 469 56 441 2.05e-15 79.0
MS.gene31546.t1 MTR_8g079710 23.467 375 228 12 77 432 59 393 2.97e-15 78.2
MS.gene31546.t1 MTR_3g435290 35.000 120 75 2 361 480 1 117 3.71e-15 72.4
MS.gene31546.t1 MTR_3g101970 22.645 499 288 21 17 484 6 437 3.74e-15 77.8
MS.gene31546.t1 MTR_7g014340 59.155 71 27 2 16 85 1 70 9.42e-15 70.5
MS.gene31546.t1 MTR_7g014205 38.136 118 53 3 336 448 18 120 1.47e-14 70.5
MS.gene31546.t1 MTR_4g094828 22.512 422 254 18 36 432 25 398 1.61e-14 75.9
MS.gene31546.t1 MTR_7g114560 22.135 384 247 13 57 431 47 387 2.61e-14 75.5
MS.gene31546.t1 MTR_3g102020 24.380 484 291 19 13 469 2 437 1.71e-13 72.8
MS.gene31546.t1 MTR_7g014220 59.375 64 25 1 32 95 10 72 2.39e-13 65.9
MS.gene31546.t1 MTR_8g079970 22.436 468 301 14 17 472 3 420 4.00e-13 71.6
MS.gene31546.t1 MTR_3g101960 23.124 493 277 22 17 469 6 436 5.77e-13 71.2
MS.gene31546.t1 MTR_7g114550 22.328 421 265 15 24 431 16 387 2.22e-12 69.3
MS.gene31546.t1 MTR_7g085990 23.197 513 300 17 16 485 1 462 4.98e-12 68.2
MS.gene31546.t1 MTR_2g007880 20.920 435 272 13 59 472 56 439 1.04e-11 67.4
MS.gene31546.t1 MTR_4g022760 24.932 369 228 13 34 392 18 347 1.13e-11 67.0
MS.gene31546.t1 MTR_7g114520 22.353 425 280 16 16 431 7 390 4.70e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31546.t1 AT1G03940 38.223 484 260 14 18 485 9 469 5.35e-103 316
MS.gene31546.t1 AT3G29670 33.473 478 277 16 15 480 1 449 5.22e-70 230
MS.gene31546.t1 AT1G03495 39.377 353 185 12 19 362 10 342 6.36e-70 226
MS.gene31546.t1 AT5G39090 34.454 476 257 18 17 475 5 442 3.32e-62 209
MS.gene31546.t1 AT5G61160 31.723 476 281 13 15 476 1 446 4.99e-61 206
MS.gene31546.t1 AT3G29680 31.857 474 280 12 15 476 1 443 6.43e-60 204
MS.gene31546.t1 AT3G29635 31.303 476 291 13 17 480 3 454 2.86e-58 199
MS.gene31546.t1 AT3G29590 32.091 483 271 18 10 476 3 444 3.87e-56 193
MS.gene31546.t1 AT5G39080 30.595 487 287 18 17 481 5 462 8.46e-56 193
MS.gene31546.t1 AT5G39050 29.897 485 296 14 17 482 10 469 5.54e-51 180
MS.gene31546.t1 AT3G48720 22.430 428 289 9 54 477 41 429 2.12e-17 84.7
MS.gene31546.t1 AT2G19070 23.650 389 255 12 59 431 40 402 3.61e-16 81.3
MS.gene31546.t1 AT3G29690 29.605 152 101 2 326 476 28 174 3.81e-16 77.0
MS.gene31546.t1 AT3G50280 25.170 441 247 18 20 432 7 392 1.24e-15 79.3
MS.gene31546.t1 AT5G67150 25.118 422 275 19 19 432 7 395 3.21e-15 78.2
MS.gene31546.t1 AT5G41040 21.925 374 254 11 71 440 63 402 1.62e-13 72.8
MS.gene31546.t1 AT5G41040 22.396 384 240 13 71 440 79 418 2.05e-13 72.8
MS.gene31546.t1 AT1G31490 23.724 392 231 14 58 432 43 383 2.21e-12 69.3

Find 98 sgRNAs with CRISPR-Local

Find 117 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATCATTTCATGAAATTT+TGG 0.059044 2.4:-11346812 MS.gene31546:CDS
GGAGGGGTGTTATGGGAATT+TGG 0.229278 2.4:+11347089 None:intergenic
TCACTTTCTTCGACATTTCA+TGG 0.242024 2.4:-11347265 MS.gene31546:CDS
TCTAGTCTACTTCATATTCT+TGG 0.285884 2.4:-11346403 MS.gene31546:CDS
ATTCACAAGATGCTCTAAAT+TGG 0.287826 2.4:-11346986 MS.gene31546:CDS
AACTCGTTTGTAGTGGTTGC+TGG 0.296286 2.4:-11346159 MS.gene31546:CDS
GATGTTTATGAGACTGATTT+TGG 0.310385 2.4:-11346123 MS.gene31546:CDS
TGCACTGCATCAGATTTCTT+TGG 0.315346 2.4:+11346090 None:intergenic
ATGATGAGGTTGGAGGAAAA+AGG 0.329263 2.4:+11347116 None:intergenic
TAAGCACTCCATCTCTTTCA+TGG 0.346789 2.4:+11346706 None:intergenic
TTGTAACATGTTCCTTGATT+TGG 0.350808 2.4:-11346476 MS.gene31546:CDS
GTATATAAGGAGGGGTGTTA+TGG 0.354673 2.4:+11347081 None:intergenic
TTTAAGAGTGATGATTTGTT+AGG 0.354935 2.4:-11346228 MS.gene31546:CDS
TGTGTTGTTGTGATTAAATT+TGG 0.361206 2.4:+11347364 None:intergenic
TCTTGTAAGTTGGTGAGTTT+AGG 0.362354 2.4:+11346669 None:intergenic
TTTAATGATTCACTATAATC+AGG 0.363977 2.4:+11346189 None:intergenic
AGGTGGAATTGAGGTTGGTT+TGG 0.381023 2.4:-11346019 MS.gene31546:CDS
CAGATAGAGAAGCCAGAGTT+TGG 0.386262 2.4:+11346873 None:intergenic
GAAGAAGTGTTGGAGGGTTA+AGG 0.387404 2.4:+11347139 None:intergenic
GTATGAGGTGAAGGCAAGTT+AGG 0.400483 2.4:+11346951 None:intergenic
GGTTGGTGATGGAAGTGAAT+TGG 0.400909 2.4:+11347298 None:intergenic
GTTGGTGATGGAAGTGAATT+GGG 0.408787 2.4:+11347299 None:intergenic
TATATAAGGAGGGGTGTTAT+GGG 0.421407 2.4:+11347082 None:intergenic
ATTTGGAGGGATGATGAGGT+TGG 0.424629 2.4:+11347106 None:intergenic
GGAAAGTGAGTGTTTGAGAA+TGG 0.424878 2.4:+11347160 None:intergenic
ATCTTGTATTTCTTGTAAGT+TGG 0.431916 2.4:+11346659 None:intergenic
GAATGGGAAGTGTTGTTTGT+AGG 0.432621 2.4:+11347177 None:intergenic
GAAGCCAGAGTTTGGTAGAA+TGG 0.432626 2.4:+11346881 None:intergenic
TCATCAAGGTAACGTATATA+AGG 0.436544 2.4:+11347068 None:intergenic
AAACTAAAGAGATGGGTTGT+TGG 0.438015 2.4:-11346537 MS.gene31546:CDS
TCTCTTTCTGATTGTAGAGA+TGG 0.439448 2.4:-11346045 MS.gene31546:CDS
GTTGAAGTCTGCTGAAGATT+CGG 0.441740 2.4:+11347022 None:intergenic
GATGAGTTGGATGAGGAAAA+TGG 0.445046 2.4:+11347204 None:intergenic
ATAAAGAGAGATGAGTTAGT+TGG 0.446078 2.4:-11346303 MS.gene31546:CDS
GTCTCATAAACATCAAGTTT+AGG 0.448315 2.4:+11346132 None:intergenic
AAAGTGAGAGGGAGAGTGGT+TGG 0.459506 2.4:+11347281 None:intergenic
TACCATACAAAACCAAATCA+AGG 0.462671 2.4:+11346464 None:intergenic
GGTGGAGGTGGAATTGAGGT+TGG 0.463005 2.4:-11346024 MS.gene31546:CDS
GAAAGTGAGTGTTTGAGAAT+GGG 0.469129 2.4:+11347161 None:intergenic
TTGGTGAGTTTAGGAGCCGT+TGG 0.469411 2.4:+11346678 None:intergenic
CTTTCTGATTGTAGAGATGG+TGG 0.471103 2.4:-11346042 MS.gene31546:CDS
GTTGTTCTTTATAGATATTG+AGG 0.473062 2.4:+11345968 None:intergenic
GCAGTGCATATTGATTCGTT+CGG 0.475920 2.4:-11346075 MS.gene31546:CDS
GAAGAAAGTGAGAGGGAGAG+TGG 0.476197 2.4:+11347277 None:intergenic
AGAGGGAGAGTGGTTGGTGA+TGG 0.482203 2.4:+11347287 None:intergenic
CAACAACACAGTTATGTTGA+AGG 0.488066 2.4:-11347351 MS.gene31546:CDS
GGGAATTTGGAGGGATGATG+AGG 0.494796 2.4:+11347102 None:intergenic
GATGGAAGTGAATTGGGTGG+TGG 0.495893 2.4:+11347305 None:intergenic
GGGATTGTTGTGGCTTCAAA+CGG 0.500064 2.4:-11346273 MS.gene31546:CDS
TGGAGGGATGATGAGGTTGG+AGG 0.507278 2.4:+11347109 None:intergenic
TACATGTATCGTTCCTCTCA+TGG 0.508285 2.4:-11346916 MS.gene31546:CDS
GTTTGTAGGAAGTGATGAGT+TGG 0.516421 2.4:+11347191 None:intergenic
TGGTAGAATGGTGATTTGAA+TGG 0.518937 2.4:+11346893 None:intergenic
ACTCGTTTGTAGTGGTTGCT+GGG 0.521923 2.4:-11346158 MS.gene31546:CDS
GGGGTGTTATGGGAATTTGG+AGG 0.522455 2.4:+11347092 None:intergenic
TGGTGAGTTTAGGAGCCGTT+GGG 0.523739 2.4:+11346679 None:intergenic
AAGCACTCCATCTCTTTCAT+GGG 0.531994 2.4:+11346707 None:intergenic
AAGTTCAGAAACTAAAGAGA+TGG 0.535160 2.4:-11346545 MS.gene31546:CDS
GATTGTAGAGATGGTGGAGG+TGG 0.541712 2.4:-11346036 MS.gene31546:CDS
AGGTTGGTTTGGTTCTTGAG+AGG 0.549471 2.4:-11346008 MS.gene31546:CDS
TGAAGATTCGGCAACGGTGA+AGG 0.551527 2.4:+11347034 None:intergenic
ACCACTACAAACGAGTTACC+TGG 0.560014 2.4:+11346165 None:intergenic
TACAACCATGTCGCTGCAGA+CGG 0.561212 2.4:-11346843 MS.gene31546:CDS
CTTCCTTCCCATGAAAGAGA+TGG 0.561446 2.4:-11346714 MS.gene31546:CDS
AGTTCAGAAACTAAAGAGAT+GGG 0.565348 2.4:-11346544 MS.gene31546:CDS
ATCACCATTCTACCAAACTC+TGG 0.574194 2.4:-11346885 MS.gene31546:CDS
CAAGGTAACGTATATAAGGA+GGG 0.577627 2.4:+11347072 None:intergenic
ACTCCATCTCTTTCATGGGA+AGG 0.579240 2.4:+11346711 None:intergenic
AATTGGGTGGTGGTGCAACT+TGG 0.579309 2.4:+11347315 None:intergenic
AGATGGTGGAGGTGGAATTG+AGG 0.584627 2.4:-11346028 MS.gene31546:CDS
AGGAAGTGATGAGTTGGATG+AGG 0.585446 2.4:+11347197 None:intergenic
CAAAGTAAACGTGATTGCAC+TGG 0.586639 2.4:-11346432 MS.gene31546:CDS
ACCAGGTAACTCGTTTGTAG+TGG 0.587043 2.4:-11346166 MS.gene31546:CDS
GGGTGTTATGGGAATTTGGA+GGG 0.588819 2.4:+11347093 None:intergenic
TCAAGGTAACGTATATAAGG+AGG 0.593641 2.4:+11347071 None:intergenic
TGCATCTCGAACGAGACTTG+TGG 0.601182 2.4:+11346614 None:intergenic
TCACCTCATACAGAACAAAA+CGG 0.603095 2.4:-11346939 MS.gene31546:CDS
GTCTGCTGAAGATTCGGCAA+CGG 0.604958 2.4:+11347028 None:intergenic
CAGCGACATGGTTGTAAGTG+AGG 0.605491 2.4:+11346850 None:intergenic
ATTTACCGTCTGCAGCGACA+TGG 0.609324 2.4:+11346838 None:intergenic
AAAAGGGAAGAAGTGTTGGA+GGG 0.615109 2.4:+11347133 None:intergenic
GGTGATGGAAGTGAATTGGG+TGG 0.622854 2.4:+11347302 None:intergenic
AGATGCAGCTTACGCTAACA+AGG 0.622869 2.4:-11346598 MS.gene31546:CDS
TCTGATTGTAGAGATGGTGG+AGG 0.625713 2.4:-11346039 MS.gene31546:CDS
TCATGGGAAGGAAGATCAAG+AGG 0.626174 2.4:+11346723 None:intergenic
TTGTCTTGCATCTTATGCTG+TGG 0.635139 2.4:-11346328 MS.gene31546:CDS
AAATGTCGAAGAAAGTGAGA+GGG 0.639421 2.4:+11347270 None:intergenic
GAAATGTCGAAGAAAGTGAG+AGG 0.646541 2.4:+11347269 None:intergenic
AAGGCAAGTTAGGAACAAGA+TGG 0.656225 2.4:+11346961 None:intergenic
AGGAGATAGAGTCTTCATCA+AGG 0.656629 2.4:+11347054 None:intergenic
GTGATTTGAATGGCCATGAG+AGG 0.670330 2.4:+11346903 None:intergenic
TATAGTGAATCATTAAAACC+AGG 0.671327 2.4:-11346183 MS.gene31546:CDS
TGGAGTGCTTAAACACCCAA+CGG 0.672231 2.4:-11346694 MS.gene31546:CDS
TGGGAAGGAAGATCAAGAGG+CGG 0.672634 2.4:+11346726 None:intergenic
AAGGTAACGTATATAAGGAG+GGG 0.677863 2.4:+11347073 None:intergenic
CATCTTGTGAATCAGATACG+AGG 0.681815 2.4:+11346997 None:intergenic
GAAGAAGATACGTTTAACAG+CGG 0.689203 2.4:+11347229 None:intergenic
ACAACAAAAGTTGACATGTG+CGG 0.758347 2.4:+11346495 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAATGATTCACTATAATC+AGG + chr2.4:11347142-11347161 None:intergenic 20.0%
!!! AGAAATACAAGATTTTGTTT+TGG - chr2.4:11346679-11346698 MS.gene31546:CDS 20.0%
!!! CAATTTCCAAAATAAGTTTT+TGG + chr2.4:11346983-11347002 None:intergenic 20.0%
!!! TCATCATTTCATGAAATTTT+GGG - chr2.4:11346517-11346536 MS.gene31546:CDS 20.0%
!!! TCATTACCAAAAACTTATTT+TGG - chr2.4:11346974-11346993 MS.gene31546:CDS 20.0%
!!! TTCATCATTTCATGAAATTT+TGG - chr2.4:11346516-11346535 MS.gene31546:CDS 20.0%
! ATCTTGTATTTCTTGTAAGT+TGG + chr2.4:11346672-11346691 None:intergenic 25.0%
! ATGAGTTAGTTGGAAAAAAT+GGG - chr2.4:11347035-11347054 MS.gene31546:CDS 25.0%
! GTTGTTCTTTATAGATATTG+AGG + chr2.4:11347363-11347382 None:intergenic 25.0%
! TATAGTGAATCATTAAAACC+AGG - chr2.4:11347145-11347164 MS.gene31546:CDS 25.0%
!! TGTGTTGTTGTGATTAAATT+TGG + chr2.4:11345967-11345986 None:intergenic 25.0%
!!! GTTGTTATTGTTTTTTGCTA+TGG + chr2.4:11346558-11346577 None:intergenic 25.0%
!!! TTTAAGAGTGATGATTTGTT+AGG - chr2.4:11347100-11347119 MS.gene31546:CDS 25.0%
AAGTTCAGAAACTAAAGAGA+TGG - chr2.4:11346783-11346802 MS.gene31546:CDS 30.0%
AGTTCAGAAACTAAAGAGAT+GGG - chr2.4:11346784-11346803 MS.gene31546:CDS 30.0%
ATAAAGAGAGATGAGTTAGT+TGG - chr2.4:11347025-11347044 MS.gene31546:CDS 30.0%
GATGAGTTAGTTGGAAAAAA+TGG - chr2.4:11347034-11347053 MS.gene31546:CDS 30.0%
GTCTCATAAACATCAAGTTT+AGG + chr2.4:11347199-11347218 None:intergenic 30.0%
TACCATACAAAACCAAATCA+AGG + chr2.4:11346867-11346886 None:intergenic 30.0%
TCATCAAGGTAACGTATATA+AGG + chr2.4:11346263-11346282 None:intergenic 30.0%
TCTAGTCTACTTCATATTCT+TGG - chr2.4:11346925-11346944 MS.gene31546:CDS 30.0%
TTGTAACATGTTCCTTGATT+TGG - chr2.4:11346852-11346871 MS.gene31546:CDS 30.0%
! ATTCACAAGATGCTCTAAAT+TGG - chr2.4:11346342-11346361 MS.gene31546:CDS 30.0%
!! GATGTTTATGAGACTGATTT+TGG - chr2.4:11347205-11347224 MS.gene31546:CDS 30.0%
!! TTATGAGACTGATTTTGGAT+GGG - chr2.4:11347210-11347229 MS.gene31546:CDS 30.0%
!! TTTATGAGACTGATTTTGGA+TGG - chr2.4:11347209-11347228 MS.gene31546:CDS 30.0%
!!! AGATTTTGTTTTGGAAGTCA+TGG - chr2.4:11346688-11346707 MS.gene31546:CDS 30.0%
!!! TTCCTTGATTTGGTTTTGTA+TGG - chr2.4:11346862-11346881 MS.gene31546:CDS 30.0%
AAATGTCGAAGAAAGTGAGA+GGG + chr2.4:11346061-11346080 None:intergenic 35.0%
AAGGTAACGTATATAAGGAG+GGG + chr2.4:11346258-11346277 None:intergenic 35.0%
ACAACAAAAGTTGACATGTG+CGG + chr2.4:11346836-11346855 None:intergenic 35.0%
CAACAACACAGTTATGTTGA+AGG - chr2.4:11345977-11345996 MS.gene31546:CDS 35.0%
CAAGGTAACGTATATAAGGA+GGG + chr2.4:11346259-11346278 None:intergenic 35.0%
GAAAGTGAGTGTTTGAGAAT+GGG + chr2.4:11346170-11346189 None:intergenic 35.0%
GAAGAAGATACGTTTAACAG+CGG + chr2.4:11346102-11346121 None:intergenic 35.0%
TCAAGGTAACGTATATAAGG+AGG + chr2.4:11346260-11346279 None:intergenic 35.0%
TCACCTCATACAGAACAAAA+CGG - chr2.4:11346389-11346408 MS.gene31546:CDS 35.0%
TCACTTTCTTCGACATTTCA+TGG - chr2.4:11346063-11346082 MS.gene31546:CDS 35.0%
TCTCTTTCTGATTGTAGAGA+TGG - chr2.4:11347283-11347302 MS.gene31546:CDS 35.0%
TGGAAAAAATGGGATTGTTG+TGG - chr2.4:11347045-11347064 MS.gene31546:CDS 35.0%
TGGTAGAATGGTGATTTGAA+TGG + chr2.4:11346438-11346457 None:intergenic 35.0%
! TTTTGTTCTGTATGAGGTGA+AGG + chr2.4:11346389-11346408 None:intergenic 35.0%
!! AAACTAAAGAGATGGGTTGT+TGG - chr2.4:11346791-11346810 MS.gene31546:CDS 35.0%
!! TATATAAGGAGGGGTGTTAT+GGG + chr2.4:11346249-11346268 None:intergenic 35.0%
!! TATGAGACTGATTTTGGATG+GGG - chr2.4:11347211-11347230 MS.gene31546:CDS 35.0%
!! TCTTGTAAGTTGGTGAGTTT+AGG + chr2.4:11346662-11346681 None:intergenic 35.0%
AAGCACTCCATCTCTTTCAT+GGG + chr2.4:11346624-11346643 None:intergenic 40.0%
AAGGCAAGTTAGGAACAAGA+TGG + chr2.4:11346370-11346389 None:intergenic 40.0%
AGGAGATAGAGTCTTCATCA+AGG + chr2.4:11346277-11346296 None:intergenic 40.0%
ATCACCATTCTACCAAACTC+TGG - chr2.4:11346443-11346462 MS.gene31546:CDS 40.0%
ATGATGAGGTTGGAGGAAAA+AGG + chr2.4:11346215-11346234 None:intergenic 40.0%
CAAAGTAAACGTGATTGCAC+TGG - chr2.4:11346896-11346915 MS.gene31546:CDS 40.0%
CATCTTGTGAATCAGATACG+AGG + chr2.4:11346334-11346353 None:intergenic 40.0%
CTTTCTGATTGTAGAGATGG+TGG - chr2.4:11347286-11347305 MS.gene31546:CDS 40.0%
GAAATGTCGAAGAAAGTGAG+AGG + chr2.4:11346062-11346081 None:intergenic 40.0%
GGAAAGTGAGTGTTTGAGAA+TGG + chr2.4:11346171-11346190 None:intergenic 40.0%
GTTGAAGTCTGCTGAAGATT+CGG + chr2.4:11346309-11346328 None:intergenic 40.0%
GTTTGTAGGAAGTGATGAGT+TGG + chr2.4:11346140-11346159 None:intergenic 40.0%
TAAGCACTCCATCTCTTTCA+TGG + chr2.4:11346625-11346644 None:intergenic 40.0%
TACATGTATCGTTCCTCTCA+TGG - chr2.4:11346412-11346431 MS.gene31546:CDS 40.0%
TGATGAGGTTGGAGGAAAAA+GGG + chr2.4:11346214-11346233 None:intergenic 40.0%
TGCACTGCATCAGATTTCTT+TGG + chr2.4:11347241-11347260 None:intergenic 40.0%
TTGTCTTGCATCTTATGCTG+TGG - chr2.4:11347000-11347019 MS.gene31546:CDS 40.0%
! AGGAAAAAGGGAAGAAGTGT+TGG + chr2.4:11346202-11346221 None:intergenic 40.0%
! GATGAGTTGGATGAGGAAAA+TGG + chr2.4:11346127-11346146 None:intergenic 40.0%
!! AAAAAGGGAAGAAGTGTTGG+AGG + chr2.4:11346199-11346218 None:intergenic 40.0%
!! AAAAGGGAAGAAGTGTTGGA+GGG + chr2.4:11346198-11346217 None:intergenic 40.0%
!! GAATGGGAAGTGTTGTTTGT+AGG + chr2.4:11346154-11346173 None:intergenic 40.0%
!! GCAGTGCATATTGATTCGTT+CGG - chr2.4:11347253-11347272 MS.gene31546:CDS 40.0%
!! GTACCGTTTTGTTCTGTATG+AGG + chr2.4:11346395-11346414 None:intergenic 40.0%
!! GTATATAAGGAGGGGTGTTA+TGG + chr2.4:11346250-11346269 None:intergenic 40.0%
!! GTTGGTGATGGAAGTGAATT+GGG + chr2.4:11346032-11346051 None:intergenic 40.0%
!! TGCTATGGCTTTTGAAAGAG+AGG + chr2.4:11346543-11346562 None:intergenic 40.0%
AACTCGTTTGTAGTGGTTGC+TGG - chr2.4:11347169-11347188 MS.gene31546:CDS 45.0%
ACCACTACAAACGAGTTACC+TGG + chr2.4:11347166-11347185 None:intergenic 45.0%
ACCAGGTAACTCGTTTGTAG+TGG - chr2.4:11347162-11347181 MS.gene31546:CDS 45.0%
ACTCCATCTCTTTCATGGGA+AGG + chr2.4:11346620-11346639 None:intergenic 45.0%
ACTCGTTTGTAGTGGTTGCT+GGG - chr2.4:11347170-11347189 MS.gene31546:CDS 45.0%
AGATGCAGCTTACGCTAACA+AGG - chr2.4:11346730-11346749 MS.gene31546:CDS 45.0%
ATTTGGAGGGATGATGAGGT+TGG + chr2.4:11346225-11346244 None:intergenic 45.0%
CAGATAGAGAAGCCAGAGTT+TGG + chr2.4:11346458-11346477 None:intergenic 45.0%
CTTCCTTCCCATGAAAGAGA+TGG - chr2.4:11346614-11346633 MS.gene31546:CDS 45.0%
GAAGCCAGAGTTTGGTAGAA+TGG + chr2.4:11346450-11346469 None:intergenic 45.0%
GGGATTGTTGTGGCTTCAAA+CGG - chr2.4:11347055-11347074 MS.gene31546:CDS 45.0%
GTATGAGGTGAAGGCAAGTT+AGG + chr2.4:11346380-11346399 None:intergenic 45.0%
GTGATTTGAATGGCCATGAG+AGG + chr2.4:11346428-11346447 None:intergenic 45.0%
TCATGGGAAGGAAGATCAAG+AGG + chr2.4:11346608-11346627 None:intergenic 45.0%
! AGGAAGTGATGAGTTGGATG+AGG + chr2.4:11346134-11346153 None:intergenic 45.0%
! AGGTGGAATTGAGGTTGGTT+TGG - chr2.4:11347309-11347328 MS.gene31546:CDS 45.0%
! GGGTGTTATGGGAATTTGGA+GGG + chr2.4:11346238-11346257 None:intergenic 45.0%
! TCTGATTGTAGAGATGGTGG+AGG - chr2.4:11347289-11347308 MS.gene31546:CDS 45.0%
! TGGAGTGCTTAAACACCCAA+CGG - chr2.4:11346634-11346653 MS.gene31546:CDS 45.0%
!! AGGTTGGTTTGGTTCTTGAG+AGG - chr2.4:11347320-11347339 MS.gene31546:CDS 45.0%
!! GAAGAAGTGTTGGAGGGTTA+AGG + chr2.4:11346192-11346211 None:intergenic 45.0%
!! GGTTGGTGATGGAAGTGAAT+TGG + chr2.4:11346033-11346052 None:intergenic 45.0%
AAAGTGAGAGGGAGAGTGGT+TGG + chr2.4:11346050-11346069 None:intergenic 50.0%
ATTTACCGTCTGCAGCGACA+TGG + chr2.4:11346493-11346512 None:intergenic 50.0%
CAGCGACATGGTTGTAAGTG+AGG + chr2.4:11346481-11346500 None:intergenic 50.0%
GAAGAAAGTGAGAGGGAGAG+TGG + chr2.4:11346054-11346073 None:intergenic 50.0%
GATGGAAGTGAATTGGGTGG+TGG + chr2.4:11346026-11346045 None:intergenic 50.0%
GGGAATTTGGAGGGATGATG+AGG + chr2.4:11346229-11346248 None:intergenic 50.0%
GTCTGCTGAAGATTCGGCAA+CGG + chr2.4:11346303-11346322 None:intergenic 50.0%
TACAACCATGTCGCTGCAGA+CGG - chr2.4:11346485-11346504 MS.gene31546:CDS 50.0%
TGAAGATTCGGCAACGGTGA+AGG + chr2.4:11346297-11346316 None:intergenic 50.0%
TGCATCTCGAACGAGACTTG+TGG + chr2.4:11346717-11346736 None:intergenic 50.0%
TGGGAAGGAAGATCAAGAGG+CGG + chr2.4:11346605-11346624 None:intergenic 50.0%
TGGTGAGTTTAGGAGCCGTT+GGG + chr2.4:11346652-11346671 None:intergenic 50.0%
TTGGTGAGTTTAGGAGCCGT+TGG + chr2.4:11346653-11346672 None:intergenic 50.0%
! AATTGGGTGGTGGTGCAACT+TGG + chr2.4:11346016-11346035 None:intergenic 50.0%
! GGGGTGTTATGGGAATTTGG+AGG + chr2.4:11346239-11346258 None:intergenic 50.0%
!! AGATGGTGGAGGTGGAATTG+AGG - chr2.4:11347300-11347319 MS.gene31546:CDS 50.0%
!! GATTGTAGAGATGGTGGAGG+TGG - chr2.4:11347292-11347311 MS.gene31546:CDS 50.0%
!! GGAGGGGTGTTATGGGAATT+TGG + chr2.4:11346242-11346261 None:intergenic 50.0%
!! GGTGATGGAAGTGAATTGGG+TGG + chr2.4:11346029-11346048 None:intergenic 50.0%
GGTGGAGGTGGAATTGAGGT+TGG - chr2.4:11347304-11347323 MS.gene31546:CDS 55.0%
TGGAGGGATGATGAGGTTGG+AGG + chr2.4:11346222-11346241 None:intergenic 55.0%
!! AGAGGGAGAGTGGTTGGTGA+TGG + chr2.4:11346044-11346063 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 11345945 11347405 11345945 ID=MS.gene31546
chr2.4 mRNA 11345945 11347405 11345945 ID=MS.gene31546.t1;Parent=MS.gene31546
chr2.4 exon 11345945 11347405 11345945 ID=MS.gene31546.t1.exon1;Parent=MS.gene31546.t1
chr2.4 CDS 11345945 11347405 11345945 ID=cds.MS.gene31546.t1;Parent=MS.gene31546.t1
Gene Sequence

>MS.gene31546

ATGAACATAACTCACGAGACCAAATTTAATCACAACAACACAGTTATGTTGAAGGTAATTGAACATAGCCAAGTTGCACCACCACCCAATTCACTTCCATCACCAACCACTCTCCCTCTCACTTTCTTCGACATTTCATGGCTTTACTGTCAATCCGCTGTTAAACGTATCTTCTTCTACCATTTTCCTCATCCAACTCATCACTTCCTACAAACAACACTTCCCATTCTCAAACACTCACTTTCCTTAACCCTCCAACACTTCTTCCCTTTTTCCTCCAACCTCATCATCCCTCCAAATTCCCATAACACCCCTCCTTATATACGTTACCTTGATGAAGACTCTATCTCCTTCACCGTTGCCGAATCTTCAGCAGACTTCAACATCCTCGTATCTGATTCACAAGATGCTCTAAATTGGCACCATCTTGTTCCTAACTTGCCTTCACCTCATACAGAACAAAACGGTACATGTATCGTTCCTCTCATGGCCATTCAAATCACCATTCTACCAAACTCTGGCTTCTCTATCTGCCTCACTTACAACCATGTCGCTGCAGACGGTAAATCACTTCATCATTTCATGAAATTTTGGGCCTCTCTTTCAAAAGCCATAGCAAAAAACAATAACAACAATAATTTGTCTCTTGAATACTCTCCGCCTCTTGATCTTCCTTCCCATGAAAGAGATGGAGTGCTTAAACACCCAACGGCTCCTAAACTCACCAACTTACAAGAAATACAAGATTTTGTTTTGGAAGTCATGGAATCCACAAGTCTCGTTCGAGATGCAGCTTACGCTAACAAGGTACGAACTAGTTTAGTATTGAGTCGTGAAGAAGTTCAGAAACTAAAGAGATGGGTTGTTGGTAAAAGTAAAGAAACAACACCGCACATGTCAACTTTTGTTGTAACATGTTCCTTGATTTGGTTTTGTATGGTAAAATCAGAGCAAAGTAAACGTGATTGCACTGGTGATGATCTAGTCTACTTCATATTCTTGGCAGATTGTCGTGATCGTTCTGAATTTTCATTACCAAAAACTTATTTTGGAAATTGTCTTGCATCTTATGCTGTGGCTATAAAGAGAGATGAGTTAGTTGGAAAAAATGGGATTGTTGTGGCTTCAAACGGTATTGAAAAGAAGATAAGAGATTTTAAGAGTGATGATTTGTTAGGAGTTGAAACGTTGATACCTGATTATAGTGAATCATTAAAACCAGGTAACTCGTTTGTAGTGGTTGCTGGGTCACCTAAACTTGATGTTTATGAGACTGATTTTGGATGGGGAAAACCAAAGAAATCTGATGCAGTGCATATTGATTCGTTCGGTTCGATTTCTCTTTCTGATTGTAGAGATGGTGGAGGTGGAATTGAGGTTGGTTTGGTTCTTGAGAGGTCTCAAATGACTAATTTCCTCAATATCTATAAAGAACAACTTAGTGATATTTGTAGCATCTGA
Protein sequence

>MS.gene31546.t1

MNITHETKFNHNNTVMLKVIEHSQVAPPPNSLPSPTTLPLTFFDISWLYCQSAVKRIFFYHFPHPTHHFLQTTLPILKHSLSLTLQHFFPFSSNLIIPPNSHNTPPYIRYLDEDSISFTVAESSADFNILVSDSQDALNWHHLVPNLPSPHTEQNGTCIVPLMAIQITILPNSGFSICLTYNHVAADGKSLHHFMKFWASLSKAIAKNNNNNNLSLEYSPPLDLPSHERDGVLKHPTAPKLTNLQEIQDFVLEVMESTSLVRDAAYANKVRTSLVLSREEVQKLKRWVVGKSKETTPHMSTFVVTCSLIWFCMVKSEQSKRDCTGDDLVYFIFLADCRDRSEFSLPKTYFGNCLASYAVAIKRDELVGKNGIVVASNGIEKKIRDFKSDDLLGVETLIPDYSESLKPGNSFVVVAGSPKLDVYETDFGWGKPKKSDAVHIDSFGSISLSDCRDGGGGIEVGLVLERSQMTNFLNIYKEQLSDICSI