AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35745

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35745.t1 MTR_2g089670 81.120 482 83 2 5 486 10 483 0.0 778
MS.gene35745.t1 MTR_2g089765 75.051 493 104 8 2 486 7 488 0.0 737
MS.gene35745.t1 MTR_8g012750 65.083 484 147 13 15 484 1 476 0.0 588
MS.gene35745.t1 MTR_2g089665 64.172 441 51 4 16 455 1 335 0.0 533
MS.gene35745.t1 MTR_7g015860 44.330 485 224 12 17 482 5 462 2.95e-123 368
MS.gene35745.t1 MTR_3g028210 36.929 482 269 11 16 483 7 467 7.78e-94 292
MS.gene35745.t1 MTR_7g014330 37.959 490 247 15 16 481 1 457 2.87e-89 280
MS.gene35745.t1 MTR_2g089650 36.515 482 267 14 17 483 9 466 3.81e-89 280
MS.gene35745.t1 MTR_6g015850 36.478 477 269 10 17 483 8 460 1.67e-86 273
MS.gene35745.t1 MTR_6g015815 36.920 474 272 10 18 483 9 463 6.61e-86 272
MS.gene35745.t1 MTR_7g014230 37.238 478 253 13 18 476 5 454 6.17e-83 264
MS.gene35745.t1 MTR_2g089755 35.546 467 253 15 40 483 37 478 2.35e-81 260
MS.gene35745.t1 MTR_6g015830 35.430 477 277 11 17 483 8 463 3.21e-80 257
MS.gene35745.t1 MTR_7g014250 35.670 485 255 15 16 476 1 452 2.89e-79 254
MS.gene35745.t1 MTR_3g087640 35.258 485 270 15 18 480 10 472 7.21e-79 254
MS.gene35745.t1 MTR_7g014200 33.874 493 278 16 12 482 4 470 2.79e-77 249
MS.gene35745.t1 MTR_2g089735 34.831 445 256 11 54 483 39 464 2.94e-77 249
MS.gene35745.t1 MTR_7g014160 34.280 493 279 15 10 480 4 473 1.31e-74 243
MS.gene35745.t1 MTR_6g015320 33.689 469 282 10 22 483 11 457 2.25e-74 241
MS.gene35745.t1 MTR_6g015315 34.713 435 261 8 54 483 39 455 1.60e-70 231
MS.gene35745.t1 MTR_7g014610 33.468 496 283 15 9 480 3 475 9.45e-70 230
MS.gene35745.t1 MTR_7g013850 31.857 474 266 17 41 480 30 480 8.81e-62 209
MS.gene35745.t1 MTR_7g014620 31.957 460 260 12 36 480 11 432 2.87e-55 191
MS.gene35745.t1 MTR_7g014360 30.788 406 230 10 87 481 22 387 1.66e-51 179
MS.gene35745.t1 MTR_3g435300 36.458 288 152 11 18 294 4 271 4.32e-42 151
MS.gene35745.t1 MTR_6g015310 29.301 372 200 9 89 458 23 333 1.05e-33 130
MS.gene35745.t1 MTR_2g089745 75.309 81 20 0 406 486 3 83 6.79e-33 120
MS.gene35745.t1 MTR_7g014630 32.069 290 163 11 17 291 9 279 3.41e-31 124
MS.gene35745.t1 MTR_7g014670 35.602 191 120 2 291 480 71 259 3.59e-31 121
MS.gene35745.t1 MTR_7g014310 29.032 341 177 10 125 463 29 306 4.90e-31 122
MS.gene35745.t1 MTR_7g014180 37.989 179 96 6 17 191 7 174 4.81e-26 104
MS.gene35745.t1 MTR_8g041770 27.295 403 247 13 37 432 27 390 1.24e-22 100
MS.gene35745.t1 MTR_4g022670 25.822 426 269 17 17 432 4 392 6.00e-20 92.4
MS.gene35745.t1 MTR_4g022720 25.971 412 249 13 34 432 18 386 1.65e-19 91.3
MS.gene35745.t1 MTR_3g101900 23.195 457 288 16 40 474 30 445 1.93e-19 91.3
MS.gene35745.t1 MTR_7g026860 25.366 410 257 14 34 432 18 389 4.61e-18 86.7
MS.gene35745.t1 MTR_4g022800 24.586 423 279 14 17 432 4 393 5.05e-17 83.6
MS.gene35745.t1 MTR_3g088420 26.887 424 236 18 29 432 15 384 1.37e-16 82.4
MS.gene35745.t1 MTR_7g014210 39.130 115 68 2 338 450 24 138 1.48e-16 77.4
MS.gene35745.t1 MTR_6g045553 25.053 475 263 18 40 469 35 461 6.07e-16 80.5
MS.gene35745.t1 MTR_7g085930 24.152 501 306 17 16 483 5 464 1.40e-15 79.3
MS.gene35745.t1 MTR_3g101930 24.048 420 272 17 63 469 56 441 1.84e-15 79.0
MS.gene35745.t1 MTR_8g079710 23.467 375 228 12 77 432 59 393 2.65e-15 78.2
MS.gene35745.t1 MTR_3g101970 22.645 499 288 21 17 484 6 437 2.96e-15 78.2
MS.gene35745.t1 MTR_3g435290 35.000 120 75 2 361 480 1 117 3.71e-15 72.4
MS.gene35745.t1 MTR_7g014340 59.155 71 27 2 16 85 1 70 9.06e-15 70.5
MS.gene35745.t1 MTR_7g014205 38.136 118 53 3 336 448 18 120 1.47e-14 70.5
MS.gene35745.t1 MTR_4g094828 22.275 422 255 18 36 432 25 398 3.34e-14 75.1
MS.gene35745.t1 MTR_7g114560 22.135 384 247 13 57 431 47 387 3.35e-14 75.1
MS.gene35745.t1 MTR_8g096530 25.000 416 261 15 76 484 58 429 8.28e-14 73.6
MS.gene35745.t1 MTR_3g102020 23.958 480 298 17 13 469 2 437 1.84e-13 72.8
MS.gene35745.t1 MTR_7g014220 59.375 64 25 1 32 95 10 72 2.37e-13 65.9
MS.gene35745.t1 MTR_8g079970 22.650 468 300 14 17 472 3 420 2.66e-13 72.0
MS.gene35745.t1 MTR_3g101960 22.921 493 278 22 17 469 6 436 1.06e-12 70.5
MS.gene35745.t1 MTR_8g075610 24.318 440 271 18 57 483 30 420 1.84e-12 69.3
MS.gene35745.t1 MTR_7g085990 23.392 513 299 17 16 485 1 462 4.14e-12 68.6
MS.gene35745.t1 MTR_2g007880 20.690 435 273 13 59 472 56 439 2.78e-11 65.9
MS.gene35745.t1 MTR_3g049150 27.119 295 169 12 160 432 129 399 7.73e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35745.t1 AT1G03940 38.430 484 259 14 18 485 9 469 1.32e-103 318
MS.gene35745.t1 AT1G03495 39.660 353 184 12 19 362 10 342 2.12e-70 228
MS.gene35745.t1 AT3G29670 33.473 478 277 16 15 480 1 449 4.41e-70 230
MS.gene35745.t1 AT5G39090 34.664 476 256 18 17 475 5 442 1.48e-62 210
MS.gene35745.t1 AT5G61160 31.328 482 287 13 15 482 1 452 3.88e-61 207
MS.gene35745.t1 AT3G29680 31.857 474 280 12 15 476 1 443 5.73e-60 204
MS.gene35745.t1 AT3G29635 31.303 476 291 13 17 480 3 454 1.71e-58 200
MS.gene35745.t1 AT3G29590 32.091 483 271 18 10 476 3 444 2.72e-56 194
MS.gene35745.t1 AT5G39080 30.390 487 288 18 17 481 5 462 2.92e-55 191
MS.gene35745.t1 AT5G39050 30.285 492 299 15 10 482 3 469 2.55e-51 181
MS.gene35745.t1 AT3G48720 22.430 428 289 9 54 477 41 429 3.28e-17 84.3
MS.gene35745.t1 AT3G29690 29.605 152 101 2 326 476 28 174 3.84e-16 77.0
MS.gene35745.t1 AT2G19070 23.650 389 255 12 59 431 40 402 4.28e-16 80.9
MS.gene35745.t1 AT3G50280 25.170 441 247 18 20 432 7 392 7.82e-16 80.1
MS.gene35745.t1 AT5G01210 24.260 338 214 10 161 474 141 460 5.00e-14 74.7
MS.gene35745.t1 AT5G41040 21.925 374 254 11 71 440 63 402 2.63e-13 72.4
MS.gene35745.t1 AT5G41040 22.396 384 240 13 71 440 79 418 3.06e-13 72.0
MS.gene35745.t1 AT1G31490 23.469 392 232 14 58 432 43 383 3.79e-12 68.6
MS.gene35745.t1 AT3G29720 30.928 194 112 7 116 305 26 201 8.74e-11 62.4

Find 97 sgRNAs with CRISPR-Local

Find 117 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATCATTTCATGAAATTT+TGG 0.059044 2.1:-12475612 MS.gene35745:CDS
GGAGGGGTGTTATGGGAATT+TGG 0.229278 2.1:+12475889 None:intergenic
TCACTTTCTTCGACATTTCA+TGG 0.242024 2.1:-12476065 MS.gene35745:CDS
TCTAGTCTACTTCATATTCT+TGG 0.285884 2.1:-12475203 MS.gene35745:CDS
ATTCACAAGATGCTCTAAAT+TGG 0.287826 2.1:-12475786 MS.gene35745:CDS
AACTCGTTTGTAGTGGTTGC+TGG 0.296286 2.1:-12474959 MS.gene35745:CDS
GATGTTTATGAGACTGATTT+TGG 0.310385 2.1:-12474923 MS.gene35745:CDS
TGCACTGCATCAGATTTCTT+TGG 0.315346 2.1:+12474890 None:intergenic
ATGATGAGGTTGGAGGAAAA+AGG 0.329263 2.1:+12475916 None:intergenic
TAAGCACTCCATCTCTTTCA+TGG 0.346789 2.1:+12475506 None:intergenic
TTGTAACATGTTCCTTGATT+TGG 0.350808 2.1:-12475276 MS.gene35745:CDS
GTATATAAGGAGGGGTGTTA+TGG 0.354673 2.1:+12475881 None:intergenic
TTTAAGAGTGATGATTTGTT+AGG 0.354935 2.1:-12475028 MS.gene35745:CDS
TGTGTTGTTGTGATTAAATT+TGG 0.361206 2.1:+12476164 None:intergenic
TCTTGTAAGTTGGTGAGTTT+AGG 0.362354 2.1:+12475469 None:intergenic
TTTAATGATTCACTATAATC+AGG 0.363977 2.1:+12474989 None:intergenic
AGGTGGAATTGAGGTTGGTT+TGG 0.381023 2.1:-12474819 MS.gene35745:CDS
CAGATAGAGAAGCCAGAGTT+TGG 0.386262 2.1:+12475673 None:intergenic
GAAGAAGTGTTGGAGGGTTA+AGG 0.387404 2.1:+12475939 None:intergenic
AAGTTCAGAAACTAAAGAAA+TGG 0.397138 2.1:-12475345 MS.gene35745:CDS
GTATGAGGTGAAGGCAAGTT+AGG 0.400483 2.1:+12475751 None:intergenic
GGTTGGTGATGGAAGTGAAT+TGG 0.400909 2.1:+12476098 None:intergenic
GTTGGTGATGGAAGTGAATT+GGG 0.408787 2.1:+12476099 None:intergenic
TATATAAGGAGGGGTGTTAT+GGG 0.421407 2.1:+12475882 None:intergenic
AAACTAAAGAAATGGGTTGT+TGG 0.424056 2.1:-12475337 MS.gene35745:CDS
ATTTGGAGGGATGATGAGGT+TGG 0.424629 2.1:+12475906 None:intergenic
GGAAAGTGAGTGTTTGAGAA+TGG 0.424878 2.1:+12475960 None:intergenic
ATCTTGTATTTCTTGTAAGT+TGG 0.431916 2.1:+12475459 None:intergenic
GAAGCCAGAGTTTGGTAGAA+TGG 0.432626 2.1:+12475681 None:intergenic
TCATCAAGGTAACGTATATA+AGG 0.436544 2.1:+12475868 None:intergenic
TCTCTTTCTGATTGTAGAGA+TGG 0.439448 2.1:-12474845 MS.gene35745:CDS
GTTGAAGTCTGCTGAAGATT+CGG 0.441740 2.1:+12475822 None:intergenic
GATGAGTTGGATGAGGAAAA+TGG 0.445046 2.1:+12476004 None:intergenic
ATAAAGAGAGATGAGTTAGT+TGG 0.446078 2.1:-12475103 MS.gene35745:CDS
GTCTCATAAACATCAAGTTT+AGG 0.448315 2.1:+12474932 None:intergenic
AAAGTGAGAGGGAGAGTGGT+TGG 0.459506 2.1:+12476081 None:intergenic
TACCATACAAAACCAAATCA+AGG 0.462671 2.1:+12475264 None:intergenic
GGTGGAGGTGGAATTGAGGT+TGG 0.463005 2.1:-12474824 MS.gene35745:CDS
GAAAGTGAGTGTTTGAGAAT+GGG 0.469129 2.1:+12475961 None:intergenic
TTGGTGAGTTTAGGAGCCGT+TGG 0.469411 2.1:+12475478 None:intergenic
CTTTCTGATTGTAGAGATGG+TGG 0.471103 2.1:-12474842 MS.gene35745:CDS
GTTGTTCTTTATAGATATTG+AGG 0.473062 2.1:+12474768 None:intergenic
GCAGTGCATATTGATTCGTT+CGG 0.475920 2.1:-12474875 MS.gene35745:CDS
GAAGAAAGTGAGAGGGAGAG+TGG 0.476197 2.1:+12476077 None:intergenic
AGAGGGAGAGTGGTTGGTGA+TGG 0.482203 2.1:+12476087 None:intergenic
AGTTCAGAAACTAAAGAAAT+GGG 0.487531 2.1:-12475344 MS.gene35745:CDS
CAACAACACAGTTATGTTGA+AGG 0.488066 2.1:-12476151 MS.gene35745:CDS
GGGAATTTGGAGGGATGATG+AGG 0.494796 2.1:+12475902 None:intergenic
GATGGAAGTGAATTGGGTGG+TGG 0.495893 2.1:+12476105 None:intergenic
GGGATTGTTGTGGCTTCAAA+CGG 0.500064 2.1:-12475073 MS.gene35745:CDS
GTTTGAAGGAAGTGATGAGT+TGG 0.500560 2.1:+12475991 None:intergenic
TGGAGGGATGATGAGGTTGG+AGG 0.507278 2.1:+12475909 None:intergenic
TACATGTATCGTTCCTCTCA+TGG 0.508285 2.1:-12475716 MS.gene35745:CDS
TGGTAGAATGGTGATTTGAA+TGG 0.518937 2.1:+12475693 None:intergenic
ACTCGTTTGTAGTGGTTGCT+GGG 0.521923 2.1:-12474958 MS.gene35745:CDS
GGGGTGTTATGGGAATTTGG+AGG 0.522455 2.1:+12475892 None:intergenic
TGGTGAGTTTAGGAGCCGTT+GGG 0.523739 2.1:+12475479 None:intergenic
AAGCACTCCATCTCTTTCAT+GGG 0.531994 2.1:+12475507 None:intergenic
GAATGGGAAGTGTTGTTTGA+AGG 0.535922 2.1:+12475977 None:intergenic
GATTGTAGAGATGGTGGAGG+TGG 0.541712 2.1:-12474836 MS.gene35745:CDS
TACAACCATGTCGCTGCAGA+CGG 0.546173 2.1:-12475643 MS.gene35745:CDS
AGGTTGGTTTGGTTCTTGAG+AGG 0.549471 2.1:-12474808 MS.gene35745:CDS
TGAAGATTCGGCAACGGTGA+AGG 0.551527 2.1:+12475834 None:intergenic
ACCACTACAAACGAGTTACC+TGG 0.560014 2.1:+12474965 None:intergenic
CTTCCTTCCCATGAAAGAGA+TGG 0.561446 2.1:-12475514 MS.gene35745:CDS
ATCACCATTCTACCAAACTC+TGG 0.574194 2.1:-12475685 MS.gene35745:CDS
CAAGGTAACGTATATAAGGA+GGG 0.577627 2.1:+12475872 None:intergenic
ACTCCATCTCTTTCATGGGA+AGG 0.579240 2.1:+12475511 None:intergenic
AATTGGGTGGTGGTGCAACT+TGG 0.579309 2.1:+12476115 None:intergenic
AGATGGTGGAGGTGGAATTG+AGG 0.584627 2.1:-12474828 MS.gene35745:CDS
CAAAGTAAACGTGATTGCAC+TGG 0.586639 2.1:-12475232 MS.gene35745:CDS
ACCAGGTAACTCGTTTGTAG+TGG 0.587043 2.1:-12474966 MS.gene35745:CDS
GGGTGTTATGGGAATTTGGA+GGG 0.588819 2.1:+12475893 None:intergenic
TCAAGGTAACGTATATAAGG+AGG 0.593641 2.1:+12475871 None:intergenic
TGCATCTCGAACGAGACTTG+TGG 0.601182 2.1:+12475414 None:intergenic
TCACCTCATACAGAACAAAA+CGG 0.603095 2.1:-12475739 MS.gene35745:CDS
GTCTGCTGAAGATTCGGCAA+CGG 0.604958 2.1:+12475828 None:intergenic
CAGCGACATGGTTGTAAGTG+AGG 0.605491 2.1:+12475650 None:intergenic
AGGAAGTGATGAGTTGGATG+AGG 0.606178 2.1:+12475997 None:intergenic
AAAAGGGAAGAAGTGTTGGA+GGG 0.615109 2.1:+12475933 None:intergenic
GGTGATGGAAGTGAATTGGG+TGG 0.622854 2.1:+12476102 None:intergenic
AGATGCAGCTTACGCTAACA+AGG 0.622869 2.1:-12475398 MS.gene35745:CDS
TCTGATTGTAGAGATGGTGG+AGG 0.625713 2.1:-12474839 MS.gene35745:CDS
TCATGGGAAGGAAGATCAAG+AGG 0.626174 2.1:+12475523 None:intergenic
TTGTCTTGCATCTTATGCTG+TGG 0.635139 2.1:-12475128 MS.gene35745:CDS
AAATGTCGAAGAAAGTGAGA+GGG 0.639421 2.1:+12476070 None:intergenic
GAAATGTCGAAGAAAGTGAG+AGG 0.646541 2.1:+12476069 None:intergenic
AAGGCAAGTTAGGAACAAGA+TGG 0.656225 2.1:+12475761 None:intergenic
AGGAGATAGAGTCTTCATCA+AGG 0.656629 2.1:+12475854 None:intergenic
GTGATTTGAATGGCCATGAG+AGG 0.670330 2.1:+12475703 None:intergenic
TATAGTGAATCATTAAAACC+AGG 0.671327 2.1:-12474983 MS.gene35745:CDS
TGGAGTGCTTAAACACCCAA+CGG 0.672231 2.1:-12475494 MS.gene35745:CDS
TGGGAAGGAAGATCAAGAGG+CGG 0.672634 2.1:+12475526 None:intergenic
AAGGTAACGTATATAAGGAG+GGG 0.677863 2.1:+12475873 None:intergenic
CATCTTGTGAATCAGATACG+AGG 0.681815 2.1:+12475797 None:intergenic
GAAGAAGATACGTTTAACAG+CGG 0.689203 2.1:+12476029 None:intergenic
ACAACAAAAGTTGACATGTG+CGG 0.758347 2.1:+12475295 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAATGATTCACTATAATC+AGG + chr2.1:12475942-12475961 None:intergenic 20.0%
!!! AGAAATACAAGATTTTGTTT+TGG - chr2.1:12475479-12475498 MS.gene35745:CDS 20.0%
!!! CAATTTCCAAAATAAGTTTT+TGG + chr2.1:12475783-12475802 None:intergenic 20.0%
!!! TCATCATTTCATGAAATTTT+GGG - chr2.1:12475317-12475336 MS.gene35745:CDS 20.0%
!!! TCATTACCAAAAACTTATTT+TGG - chr2.1:12475774-12475793 MS.gene35745:CDS 20.0%
!!! TTCATCATTTCATGAAATTT+TGG - chr2.1:12475316-12475335 MS.gene35745:CDS 20.0%
! AAGTTCAGAAACTAAAGAAA+TGG - chr2.1:12475583-12475602 MS.gene35745:CDS 25.0%
! AGTTCAGAAACTAAAGAAAT+GGG - chr2.1:12475584-12475603 MS.gene35745:CDS 25.0%
! ATCTTGTATTTCTTGTAAGT+TGG + chr2.1:12475472-12475491 None:intergenic 25.0%
! ATGAGTTAGTTGGAAAAAAT+GGG - chr2.1:12475835-12475854 MS.gene35745:CDS 25.0%
! GTTGTTCTTTATAGATATTG+AGG + chr2.1:12476163-12476182 None:intergenic 25.0%
! TATAGTGAATCATTAAAACC+AGG - chr2.1:12475945-12475964 MS.gene35745:CDS 25.0%
!! TGTGTTGTTGTGATTAAATT+TGG + chr2.1:12474767-12474786 None:intergenic 25.0%
!!! GTTGTTATTGTTTTTTGCTA+TGG + chr2.1:12475358-12475377 None:intergenic 25.0%
!!! TTTAAGAGTGATGATTTGTT+AGG - chr2.1:12475900-12475919 MS.gene35745:CDS 25.0%
ATAAAGAGAGATGAGTTAGT+TGG - chr2.1:12475825-12475844 MS.gene35745:CDS 30.0%
GATGAGTTAGTTGGAAAAAA+TGG - chr2.1:12475834-12475853 MS.gene35745:CDS 30.0%
GTCTCATAAACATCAAGTTT+AGG + chr2.1:12475999-12476018 None:intergenic 30.0%
TACCATACAAAACCAAATCA+AGG + chr2.1:12475667-12475686 None:intergenic 30.0%
TCATCAAGGTAACGTATATA+AGG + chr2.1:12475063-12475082 None:intergenic 30.0%
TCTAGTCTACTTCATATTCT+TGG - chr2.1:12475725-12475744 MS.gene35745:CDS 30.0%
TTGTAACATGTTCCTTGATT+TGG - chr2.1:12475652-12475671 MS.gene35745:CDS 30.0%
! ATTCACAAGATGCTCTAAAT+TGG - chr2.1:12475142-12475161 MS.gene35745:CDS 30.0%
!! AAACTAAAGAAATGGGTTGT+TGG - chr2.1:12475591-12475610 MS.gene35745:CDS 30.0%
!! GATGTTTATGAGACTGATTT+TGG - chr2.1:12476005-12476024 MS.gene35745:CDS 30.0%
!! TTATGAGACTGATTTTGGAT+GGG - chr2.1:12476010-12476029 MS.gene35745:CDS 30.0%
!! TTTATGAGACTGATTTTGGA+TGG - chr2.1:12476009-12476028 MS.gene35745:CDS 30.0%
!!! AGATTTTGTTTTGGAAGTCA+TGG - chr2.1:12475488-12475507 MS.gene35745:CDS 30.0%
!!! TTCCTTGATTTGGTTTTGTA+TGG - chr2.1:12475662-12475681 MS.gene35745:CDS 30.0%
AAATGTCGAAGAAAGTGAGA+GGG + chr2.1:12474861-12474880 None:intergenic 35.0%
AAGGTAACGTATATAAGGAG+GGG + chr2.1:12475058-12475077 None:intergenic 35.0%
ACAACAAAAGTTGACATGTG+CGG + chr2.1:12475636-12475655 None:intergenic 35.0%
CAACAACACAGTTATGTTGA+AGG - chr2.1:12474777-12474796 MS.gene35745:CDS 35.0%
CAAGGTAACGTATATAAGGA+GGG + chr2.1:12475059-12475078 None:intergenic 35.0%
GAAAGTGAGTGTTTGAGAAT+GGG + chr2.1:12474970-12474989 None:intergenic 35.0%
GAAGAAGATACGTTTAACAG+CGG + chr2.1:12474902-12474921 None:intergenic 35.0%
TCAAGGTAACGTATATAAGG+AGG + chr2.1:12475060-12475079 None:intergenic 35.0%
TCACCTCATACAGAACAAAA+CGG - chr2.1:12475189-12475208 MS.gene35745:CDS 35.0%
TCACTTTCTTCGACATTTCA+TGG - chr2.1:12474863-12474882 MS.gene35745:CDS 35.0%
TCTCTTTCTGATTGTAGAGA+TGG - chr2.1:12476083-12476102 MS.gene35745:CDS 35.0%
TGGAAAAAATGGGATTGTTG+TGG - chr2.1:12475845-12475864 MS.gene35745:CDS 35.0%
TGGTAGAATGGTGATTTGAA+TGG + chr2.1:12475238-12475257 None:intergenic 35.0%
! TTTTGTTCTGTATGAGGTGA+AGG + chr2.1:12475189-12475208 None:intergenic 35.0%
!! TATATAAGGAGGGGTGTTAT+GGG + chr2.1:12475049-12475068 None:intergenic 35.0%
!! TATGAGACTGATTTTGGATG+GGG - chr2.1:12476011-12476030 MS.gene35745:CDS 35.0%
!! TCTTGTAAGTTGGTGAGTTT+AGG + chr2.1:12475462-12475481 None:intergenic 35.0%
AAGCACTCCATCTCTTTCAT+GGG + chr2.1:12475424-12475443 None:intergenic 40.0%
AAGGCAAGTTAGGAACAAGA+TGG + chr2.1:12475170-12475189 None:intergenic 40.0%
AGGAGATAGAGTCTTCATCA+AGG + chr2.1:12475077-12475096 None:intergenic 40.0%
ATCACCATTCTACCAAACTC+TGG - chr2.1:12475243-12475262 MS.gene35745:CDS 40.0%
ATGATGAGGTTGGAGGAAAA+AGG + chr2.1:12475015-12475034 None:intergenic 40.0%
CAAAGTAAACGTGATTGCAC+TGG - chr2.1:12475696-12475715 MS.gene35745:CDS 40.0%
CATCTTGTGAATCAGATACG+AGG + chr2.1:12475134-12475153 None:intergenic 40.0%
CTTTCTGATTGTAGAGATGG+TGG - chr2.1:12476086-12476105 MS.gene35745:CDS 40.0%
GAAATGTCGAAGAAAGTGAG+AGG + chr2.1:12474862-12474881 None:intergenic 40.0%
GGAAAGTGAGTGTTTGAGAA+TGG + chr2.1:12474971-12474990 None:intergenic 40.0%
GTTGAAGTCTGCTGAAGATT+CGG + chr2.1:12475109-12475128 None:intergenic 40.0%
GTTTGAAGGAAGTGATGAGT+TGG + chr2.1:12474940-12474959 None:intergenic 40.0%
TAAGCACTCCATCTCTTTCA+TGG + chr2.1:12475425-12475444 None:intergenic 40.0%
TACATGTATCGTTCCTCTCA+TGG - chr2.1:12475212-12475231 MS.gene35745:CDS 40.0%
TGATGAGGTTGGAGGAAAAA+GGG + chr2.1:12475014-12475033 None:intergenic 40.0%
TGCACTGCATCAGATTTCTT+TGG + chr2.1:12476041-12476060 None:intergenic 40.0%
TTGTCTTGCATCTTATGCTG+TGG - chr2.1:12475800-12475819 MS.gene35745:CDS 40.0%
! AGGAAAAAGGGAAGAAGTGT+TGG + chr2.1:12475002-12475021 None:intergenic 40.0%
! GATGAGTTGGATGAGGAAAA+TGG + chr2.1:12474927-12474946 None:intergenic 40.0%
! TGCTATGGCTTTTGTAAGAG+AGG + chr2.1:12475343-12475362 None:intergenic 40.0%
!! AAAAAGGGAAGAAGTGTTGG+AGG + chr2.1:12474999-12475018 None:intergenic 40.0%
!! AAAAGGGAAGAAGTGTTGGA+GGG + chr2.1:12474998-12475017 None:intergenic 40.0%
!! GAATGGGAAGTGTTGTTTGA+AGG + chr2.1:12474954-12474973 None:intergenic 40.0%
!! GCAGTGCATATTGATTCGTT+CGG - chr2.1:12476053-12476072 MS.gene35745:CDS 40.0%
!! GTACCGTTTTGTTCTGTATG+AGG + chr2.1:12475195-12475214 None:intergenic 40.0%
!! GTATATAAGGAGGGGTGTTA+TGG + chr2.1:12475050-12475069 None:intergenic 40.0%
!! GTTGGTGATGGAAGTGAATT+GGG + chr2.1:12474832-12474851 None:intergenic 40.0%
AACTCGTTTGTAGTGGTTGC+TGG - chr2.1:12475969-12475988 MS.gene35745:CDS 45.0%
ACCACTACAAACGAGTTACC+TGG + chr2.1:12475966-12475985 None:intergenic 45.0%
ACCAGGTAACTCGTTTGTAG+TGG - chr2.1:12475962-12475981 MS.gene35745:CDS 45.0%
ACTCCATCTCTTTCATGGGA+AGG + chr2.1:12475420-12475439 None:intergenic 45.0%
ACTCGTTTGTAGTGGTTGCT+GGG - chr2.1:12475970-12475989 MS.gene35745:CDS 45.0%
AGATGCAGCTTACGCTAACA+AGG - chr2.1:12475530-12475549 MS.gene35745:CDS 45.0%
ATTTGGAGGGATGATGAGGT+TGG + chr2.1:12475025-12475044 None:intergenic 45.0%
CAGATAGAGAAGCCAGAGTT+TGG + chr2.1:12475258-12475277 None:intergenic 45.0%
CTTCCTTCCCATGAAAGAGA+TGG - chr2.1:12475414-12475433 MS.gene35745:CDS 45.0%
GAAGCCAGAGTTTGGTAGAA+TGG + chr2.1:12475250-12475269 None:intergenic 45.0%
GGGATTGTTGTGGCTTCAAA+CGG - chr2.1:12475855-12475874 MS.gene35745:CDS 45.0%
GTATGAGGTGAAGGCAAGTT+AGG + chr2.1:12475180-12475199 None:intergenic 45.0%
GTGATTTGAATGGCCATGAG+AGG + chr2.1:12475228-12475247 None:intergenic 45.0%
TCATGGGAAGGAAGATCAAG+AGG + chr2.1:12475408-12475427 None:intergenic 45.0%
! AGGAAGTGATGAGTTGGATG+AGG + chr2.1:12474934-12474953 None:intergenic 45.0%
! AGGTGGAATTGAGGTTGGTT+TGG - chr2.1:12476109-12476128 MS.gene35745:CDS 45.0%
! GGGTGTTATGGGAATTTGGA+GGG + chr2.1:12475038-12475057 None:intergenic 45.0%
! TCTGATTGTAGAGATGGTGG+AGG - chr2.1:12476089-12476108 MS.gene35745:CDS 45.0%
! TGGAGTGCTTAAACACCCAA+CGG - chr2.1:12475434-12475453 MS.gene35745:CDS 45.0%
!! AGGTTGGTTTGGTTCTTGAG+AGG - chr2.1:12476120-12476139 MS.gene35745:CDS 45.0%
!! GAAGAAGTGTTGGAGGGTTA+AGG + chr2.1:12474992-12475011 None:intergenic 45.0%
!! GGTTGGTGATGGAAGTGAAT+TGG + chr2.1:12474833-12474852 None:intergenic 45.0%
AAAGTGAGAGGGAGAGTGGT+TGG + chr2.1:12474850-12474869 None:intergenic 50.0%
CAGCGACATGGTTGTAAGTG+AGG + chr2.1:12475281-12475300 None:intergenic 50.0%
GAAGAAAGTGAGAGGGAGAG+TGG + chr2.1:12474854-12474873 None:intergenic 50.0%
GATGGAAGTGAATTGGGTGG+TGG + chr2.1:12474826-12474845 None:intergenic 50.0%
GGGAATTTGGAGGGATGATG+AGG + chr2.1:12475029-12475048 None:intergenic 50.0%
GTCTGCTGAAGATTCGGCAA+CGG + chr2.1:12475103-12475122 None:intergenic 50.0%
TACAACCATGTCGCTGCAGA+CGG - chr2.1:12475285-12475304 MS.gene35745:CDS 50.0%
TGAAGATTCGGCAACGGTGA+AGG + chr2.1:12475097-12475116 None:intergenic 50.0%
TGCATCTCGAACGAGACTTG+TGG + chr2.1:12475517-12475536 None:intergenic 50.0%
TGGGAAGGAAGATCAAGAGG+CGG + chr2.1:12475405-12475424 None:intergenic 50.0%
TGGTGAGTTTAGGAGCCGTT+GGG + chr2.1:12475452-12475471 None:intergenic 50.0%
TTGGTGAGTTTAGGAGCCGT+TGG + chr2.1:12475453-12475472 None:intergenic 50.0%
! AATTGGGTGGTGGTGCAACT+TGG + chr2.1:12474816-12474835 None:intergenic 50.0%
! ATTTTCCGTCTGCAGCGACA+TGG + chr2.1:12475293-12475312 None:intergenic 50.0%
! GGGGTGTTATGGGAATTTGG+AGG + chr2.1:12475039-12475058 None:intergenic 50.0%
!! AGATGGTGGAGGTGGAATTG+AGG - chr2.1:12476100-12476119 MS.gene35745:CDS 50.0%
!! GATTGTAGAGATGGTGGAGG+TGG - chr2.1:12476092-12476111 MS.gene35745:CDS 50.0%
!! GGAGGGGTGTTATGGGAATT+TGG + chr2.1:12475042-12475061 None:intergenic 50.0%
!! GGTGATGGAAGTGAATTGGG+TGG + chr2.1:12474829-12474848 None:intergenic 50.0%
GGTGGAGGTGGAATTGAGGT+TGG - chr2.1:12476104-12476123 MS.gene35745:CDS 55.0%
TGGAGGGATGATGAGGTTGG+AGG + chr2.1:12475022-12475041 None:intergenic 55.0%
!! AGAGGGAGAGTGGTTGGTGA+TGG + chr2.1:12474844-12474863 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 12474745 12476205 12474745 ID=MS.gene35745
chr2.1 mRNA 12474745 12476205 12474745 ID=MS.gene35745.t1;Parent=MS.gene35745
chr2.1 exon 12474745 12476205 12474745 ID=MS.gene35745.t1.exon1;Parent=MS.gene35745.t1
chr2.1 CDS 12474745 12476205 12474745 ID=cds.MS.gene35745.t1;Parent=MS.gene35745.t1
Gene Sequence

>MS.gene35745

ATGAACATAACTCACGAGACCAAATTTAATCACAACAACACAGTTATGTTGAAGGTAATTGAACATAGCCAAGTTGCACCACCACCCAATTCACTTCCATCACCAACCACTCTCCCTCTCACTTTCTTCGACATTTCATGGCTTTACTGTCAATCCGCTGTTAAACGTATCTTCTTCTACCATTTTCCTCATCCAACTCATCACTTCCTTCAAACAACACTTCCCATTCTCAAACACTCACTTTCCTTAACCCTCCAACACTTCTTCCCTTTTTCCTCCAACCTCATCATCCCTCCAAATTCCCATAACACCCCTCCTTATATACGTTACCTTGATGAAGACTCTATCTCCTTCACCGTTGCCGAATCTTCAGCAGACTTCAACATCCTCGTATCTGATTCACAAGATGCTCTAAATTGGCACCATCTTGTTCCTAACTTGCCTTCACCTCATACAGAACAAAACGGTACATGTATCGTTCCTCTCATGGCCATTCAAATCACCATTCTACCAAACTCTGGCTTCTCTATCTGCCTCACTTACAACCATGTCGCTGCAGACGGAAAATCACTTCATCATTTCATGAAATTTTGGGCCTCTCTTACAAAAGCCATAGCAAAAAACAATAACAACAATAATTTGTCTCTTGAATACTCTCCGCCTCTTGATCTTCCTTCCCATGAAAGAGATGGAGTGCTTAAACACCCAACGGCTCCTAAACTCACCAACTTACAAGAAATACAAGATTTTGTTTTGGAAGTCATGGAATCCACAAGTCTCGTTCGAGATGCAGCTTACGCTAACAAGGTACGAACTAGTTTAGTATTGAGTCGTGAAGAAGTTCAGAAACTAAAGAAATGGGTTGTTGGTAAAAGTAAAGAAACAACACCGCACATGTCAACTTTTGTTGTAACATGTTCCTTGATTTGGTTTTGTATGGTAAAATCAGAGCAAAGTAAACGTGATTGCACTGGTGATGATCTAGTCTACTTCATATTCTTGGCAGATTGTCGTGATCGTTCTGAATTTTCATTACCAAAAACTTATTTTGGAAATTGTCTTGCATCTTATGCTGTGGCTATAAAGAGAGATGAGTTAGTTGGAAAAAATGGGATTGTTGTGGCTTCAAACGGTATTGAAAAGAAGATAAGAGATTTTAAGAGTGATGATTTGTTAGGAGTTGAAACGTTGATACCTGATTATAGTGAATCATTAAAACCAGGTAACTCGTTTGTAGTGGTTGCTGGGTCACCTAAACTTGATGTTTATGAGACTGATTTTGGATGGGGAAAACCAAAGAAATCTGATGCAGTGCATATTGATTCGTTCGGTTCGATTTCTCTTTCTGATTGTAGAGATGGTGGAGGTGGAATTGAGGTTGGTTTGGTTCTTGAGAGGTCTCAAATGACTAATTTCCTCAATATCTATAAAGAACAACTTAGTGATATTTGTAGCATCTGA
Protein sequence

>MS.gene35745.t1

MNITHETKFNHNNTVMLKVIEHSQVAPPPNSLPSPTTLPLTFFDISWLYCQSAVKRIFFYHFPHPTHHFLQTTLPILKHSLSLTLQHFFPFSSNLIIPPNSHNTPPYIRYLDEDSISFTVAESSADFNILVSDSQDALNWHHLVPNLPSPHTEQNGTCIVPLMAIQITILPNSGFSICLTYNHVAADGKSLHHFMKFWASLTKAIAKNNNNNNLSLEYSPPLDLPSHERDGVLKHPTAPKLTNLQEIQDFVLEVMESTSLVRDAAYANKVRTSLVLSREEVQKLKKWVVGKSKETTPHMSTFVVTCSLIWFCMVKSEQSKRDCTGDDLVYFIFLADCRDRSEFSLPKTYFGNCLASYAVAIKRDELVGKNGIVVASNGIEKKIRDFKSDDLLGVETLIPDYSESLKPGNSFVVVAGSPKLDVYETDFGWGKPKKSDAVHIDSFGSISLSDCRDGGGGIEVGLVLERSQMTNFLNIYKEQLSDICSI