Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36384.t1 | XP_013452417.1 | 97.9 | 95 | 2 | 0 | 3 | 97 | 198 | 292 | 1.50E-45 | 191.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36384.t1 | Q9FHM7 | 86.3 | 95 | 12 | 1 | 3 | 96 | 198 | 292 | 7.1e-41 | 167.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36384.t1 | I3SG23 | 97.9 | 95 | 2 | 0 | 3 | 97 | 198 | 292 | 1.1e-45 | 191.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene36384 | 0.829056 | 6.37E-55 | -1.69E-46 |
| MS.gene049230 | MS.gene36384 | 0.81593 | 7.18E-52 | -1.69E-46 |
| MS.gene049419 | MS.gene36384 | -0.807648 | 4.56E-50 | -1.69E-46 |
| MS.gene049452 | MS.gene36384 | 0.801278 | 9.71E-49 | -1.69E-46 |
| MS.gene050105 | MS.gene36384 | 0.8118 | 5.84E-51 | -1.69E-46 |
| MS.gene050355 | MS.gene36384 | 0.806653 | 7.41E-50 | -1.69E-46 |
| MS.gene050605 | MS.gene36384 | 0.84122 | 5.42E-58 | -1.69E-46 |
| MS.gene050953 | MS.gene36384 | 0.801223 | 9.96E-49 | -1.69E-46 |
| MS.gene051122 | MS.gene36384 | 0.807181 | 5.73E-50 | -1.69E-46 |
| MS.gene051368 | MS.gene36384 | 0.806444 | 8.20E-50 | -1.69E-46 |
| MS.gene051452 | MS.gene36384 | 0.812415 | 4.29E-51 | -1.69E-46 |
| MS.gene051975 | MS.gene36384 | 0.806968 | 6.36E-50 | -1.69E-46 |
| MS.gene052012 | MS.gene36384 | 0.811294 | 7.52E-51 | -1.69E-46 |
| MS.gene052426 | MS.gene36384 | 0.829785 | 4.24E-55 | -1.69E-46 |
| MS.gene052586 | MS.gene36384 | 0.807446 | 5.03E-50 | -1.69E-46 |
| MS.gene052674 | MS.gene36384 | 0.805175 | 1.52E-49 | -1.69E-46 |
| MS.gene052675 | MS.gene36384 | 0.801122 | 1.04E-48 | -1.69E-46 |
| MS.gene052677 | MS.gene36384 | 0.80615 | 9.46E-50 | -1.69E-46 |
| MS.gene052954 | MS.gene36384 | 0.834917 | 2.27E-56 | -1.69E-46 |
| MS.gene053006 | MS.gene36384 | 0.802656 | 5.06E-49 | -1.69E-46 |
| MS.gene053008 | MS.gene36384 | 0.800123 | 1.67E-48 | -1.69E-46 |
| MS.gene053166 | MS.gene36384 | 0.804437 | 2.16E-49 | -1.69E-46 |
| MS.gene053216 | MS.gene36384 | 0.800436 | 1.44E-48 | -1.69E-46 |
| MS.gene053931 | MS.gene36384 | 0.8151 | 1.10E-51 | -1.69E-46 |
| MS.gene053963 | MS.gene36384 | 0.800827 | 1.20E-48 | -1.69E-46 |
| MS.gene054236 | MS.gene36384 | 0.828763 | 7.50E-55 | -1.69E-46 |
| MS.gene054479 | MS.gene36384 | 0.832675 | 8.26E-56 | -1.69E-46 |
| MS.gene054657 | MS.gene36384 | 0.807162 | 5.78E-50 | -1.69E-46 |
| MS.gene05510 | MS.gene36384 | 0.834497 | 2.90E-56 | -1.69E-46 |
| MS.gene055222 | MS.gene36384 | 0.815281 | 1.00E-51 | -1.69E-46 |
| MS.gene055623 | MS.gene36384 | 0.813186 | 2.91E-51 | -1.69E-46 |
| MS.gene055701 | MS.gene36384 | 0.841466 | 4.67E-58 | -1.69E-46 |
| MS.gene055841 | MS.gene36384 | 0.808483 | 3.03E-50 | -1.69E-46 |
| MS.gene056506 | MS.gene36384 | 0.820349 | 7.19E-53 | -1.69E-46 |
| MS.gene056986 | MS.gene36384 | 0.808476 | 3.04E-50 | -1.69E-46 |
| MS.gene057208 | MS.gene36384 | 0.819274 | 1.27E-52 | -1.69E-46 |
| MS.gene057693 | MS.gene36384 | 0.808458 | 3.07E-50 | -1.69E-46 |
| MS.gene058146 | MS.gene36384 | 0.802657 | 5.06E-49 | -1.69E-46 |
| MS.gene058548 | MS.gene36384 | 0.817906 | 2.59E-52 | -1.69E-46 |
| MS.gene058549 | MS.gene36384 | 0.810032 | 1.41E-50 | -1.69E-46 |
| MS.gene058595 | MS.gene36384 | 0.835082 | 2.06E-56 | -1.69E-46 |
| MS.gene058783 | MS.gene36384 | 0.807522 | 4.85E-50 | -1.69E-46 |
| MS.gene058979 | MS.gene36384 | 0.812331 | 4.47E-51 | -1.69E-46 |
| MS.gene059283 | MS.gene36384 | 0.800503 | 1.40E-48 | -1.69E-46 |
| MS.gene059317 | MS.gene36384 | 0.809208 | 2.12E-50 | -1.69E-46 |
| MS.gene05979 | MS.gene36384 | 0.819448 | 1.16E-52 | -1.69E-46 |
| MS.gene059830 | MS.gene36384 | 0.809291 | 2.03E-50 | -1.69E-46 |
| MS.gene059842 | MS.gene36384 | 0.810231 | 1.28E-50 | -1.69E-46 |
| MS.gene060011 | MS.gene36384 | 0.808113 | 3.63E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene36384 | 0.809178 | 2.15E-50 | -1.69E-46 |
| MS.gene060586 | MS.gene36384 | 0.800175 | 1.63E-48 | -1.69E-46 |
| MS.gene060802 | MS.gene36384 | 0.814781 | 1.29E-51 | -1.69E-46 |
| MS.gene060803 | MS.gene36384 | 0.806954 | 6.40E-50 | -1.69E-46 |
| MS.gene060935 | MS.gene36384 | 0.826835 | 2.18E-54 | -1.69E-46 |
| MS.gene060937 | MS.gene36384 | 0.812748 | 3.63E-51 | -1.69E-46 |
| MS.gene060998 | MS.gene36384 | 0.820169 | 7.91E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36384.t1 | MTR_6g460470 | 97.895 | 95 | 2 | 0 | 3 | 97 | 198 | 292 | 3.67e-64 | 196 |
| MS.gene36384.t1 | MTR_6g011380 | 57.303 | 89 | 38 | 0 | 3 | 91 | 196 | 284 | 1.59e-36 | 125 |
| MS.gene36384.t1 | MTR_4g107720 | 57.471 | 87 | 37 | 0 | 3 | 89 | 196 | 282 | 1.93e-35 | 122 |
| MS.gene36384.t1 | MTR_4g107730 | 59.770 | 87 | 35 | 0 | 3 | 89 | 194 | 280 | 2.70e-35 | 121 |
| MS.gene36384.t1 | MTR_4g107810 | 56.322 | 87 | 38 | 0 | 3 | 89 | 196 | 282 | 7.16e-35 | 120 |
| MS.gene36384.t1 | MTR_4g107760 | 56.322 | 87 | 38 | 0 | 3 | 89 | 196 | 282 | 7.16e-35 | 120 |
| MS.gene36384.t1 | MTR_8g469160 | 52.222 | 90 | 43 | 0 | 3 | 92 | 196 | 285 | 8.32e-35 | 120 |
| MS.gene36384.t1 | MTR_4g107810 | 56.322 | 87 | 38 | 0 | 3 | 89 | 256 | 342 | 3.19e-34 | 120 |
| MS.gene36384.t1 | MTR_4g107830 | 55.172 | 87 | 39 | 0 | 3 | 89 | 211 | 297 | 1.45e-33 | 117 |
| MS.gene36384.t1 | MTR_4g107700 | 56.790 | 81 | 35 | 0 | 9 | 89 | 6 | 86 | 2.72e-33 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36384.t1 | AT5G51570 | 86.316 | 95 | 12 | 1 | 3 | 96 | 198 | 292 | 9.15e-55 | 172 |
| MS.gene36384.t1 | AT3G01290 | 59.551 | 89 | 36 | 0 | 3 | 91 | 196 | 284 | 9.20e-37 | 125 |
| MS.gene36384.t1 | AT1G69840 | 57.303 | 89 | 38 | 0 | 3 | 91 | 196 | 284 | 2.67e-36 | 124 |
| MS.gene36384.t1 | AT1G69840 | 57.303 | 89 | 38 | 0 | 3 | 91 | 196 | 284 | 2.67e-36 | 124 |
| MS.gene36384.t1 | AT1G69840 | 57.303 | 89 | 38 | 0 | 3 | 91 | 196 | 284 | 2.67e-36 | 124 |
| MS.gene36384.t1 | AT1G69840 | 57.303 | 89 | 38 | 0 | 3 | 91 | 196 | 284 | 2.67e-36 | 124 |
| MS.gene36384.t1 | AT1G69840 | 57.303 | 89 | 38 | 0 | 3 | 91 | 196 | 284 | 2.67e-36 | 124 |
| MS.gene36384.t1 | AT1G69840 | 57.303 | 89 | 38 | 0 | 3 | 91 | 196 | 284 | 2.67e-36 | 124 |
| MS.gene36384.t1 | AT1G69840 | 57.303 | 89 | 38 | 0 | 3 | 91 | 196 | 284 | 2.67e-36 | 124 |
| MS.gene36384.t1 | AT5G62740 | 54.945 | 91 | 41 | 0 | 3 | 93 | 196 | 286 | 6.79e-36 | 123 |
Find 16 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTGGCCATGTTAGGGATAT+TGG | 0.321495 | 2.2:+2656232 | MS.gene36384:CDS |
| CTCATGGACCTGGCCATGTT+AGG | 0.353338 | 2.2:+2656224 | MS.gene36384:CDS |
| TCATGGACCTGGCCATGTTA+GGG | 0.460907 | 2.2:+2656225 | MS.gene36384:CDS |
| TATTCTTTGAATTGTTTCCA+AGG | 0.488072 | 2.2:-2656186 | None:intergenic |
| TTTGACACAATCAGAGACCT+TGG | 0.519786 | 2.2:+2656169 | MS.gene36384:CDS |
| AACTTCAGCAAAGGAAGTGA+TGG | 0.519872 | 2.2:+2656123 | MS.gene36384:CDS |
| CCAATATCCCTAACATGGCC+AGG | 0.522093 | 2.2:-2656232 | None:intergenic |
| TCAAATTCGCAACGGTGTGA+TGG | 0.541511 | 2.2:+2656258 | MS.gene36384:CDS |
| CAGCGACAAGCTATCACAGA+TGG | 0.616981 | 2.2:+2656058 | MS.gene36384:CDS |
| CAGTTCTCTAACAAGGTGGA+AGG | 0.624094 | 2.2:+2656100 | MS.gene36384:CDS |
| TATCTTGCAGTTCTCTAACA+AGG | 0.628125 | 2.2:+2656093 | MS.gene36384:CDS |
| GATCACCAATATCCCTAACA+TGG | 0.643726 | 2.2:-2656237 | None:intergenic |
| GGTGGAAGGAACTTCAGCAA+AGG | 0.646544 | 2.2:+2656114 | MS.gene36384:CDS |
| CTTGCAGTTCTCTAACAAGG+TGG | 0.672774 | 2.2:+2656096 | MS.gene36384:CDS |
| ATTGGTGATCAAATTCGCAA+CGG | 0.676665 | 2.2:+2656250 | MS.gene36384:CDS |
| CTAACATGGCCAGGTCCATG+AGG | 0.693167 | 2.2:-2656223 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TATTCTTTGAATTGTTTCCA+AGG | - | chr2.2:2656189-2656208 | None:intergenic | 25.0% |
| TATCTTGCAGTTCTCTAACA+AGG | + | chr2.2:2656093-2656112 | MS.gene36384:CDS | 35.0% | |
| TCCATGAGGTATAAAAACTG+TGG | - | chr2.2:2656212-2656231 | None:intergenic | 35.0% | |
| ! | ACCACAGTTTTTATACCTCA+TGG | + | chr2.2:2656208-2656227 | MS.gene36384:CDS | 35.0% |
| ! | ATTGGTGATCAAATTCGCAA+CGG | + | chr2.2:2656250-2656269 | MS.gene36384:CDS | 35.0% |
| AACTTCAGCAAAGGAAGTGA+TGG | + | chr2.2:2656123-2656142 | MS.gene36384:CDS | 40.0% | |
| GATCACCAATATCCCTAACA+TGG | - | chr2.2:2656240-2656259 | None:intergenic | 40.0% | |
| TTTGACACAATCAGAGACCT+TGG | + | chr2.2:2656169-2656188 | MS.gene36384:CDS | 40.0% | |
| ! | GTTTTTATACCTCATGGACC+TGG | + | chr2.2:2656214-2656233 | MS.gene36384:CDS | 40.0% |
| !! | TTGGAGTTTTTAGGAGGAGT+TGG | + | chr2.2:2656025-2656044 | MS.gene36384:CDS | 40.0% |
| CAGTTCTCTAACAAGGTGGA+AGG | + | chr2.2:2656100-2656119 | MS.gene36384:CDS | 45.0% | |
| CTTGCAGTTCTCTAACAAGG+TGG | + | chr2.2:2656096-2656115 | MS.gene36384:CDS | 45.0% | |
| TCAAATTCGCAACGGTGTGA+TGG | + | chr2.2:2656258-2656277 | MS.gene36384:CDS | 45.0% | |
| !!! | GTTTTTAGGAGGAGTTGGTG+TGG | + | chr2.2:2656030-2656049 | MS.gene36384:CDS | 45.0% |
| CAGCGACAAGCTATCACAGA+TGG | + | chr2.2:2656058-2656077 | MS.gene36384:CDS | 50.0% | |
| CCAATATCCCTAACATGGCC+AGG | - | chr2.2:2656235-2656254 | None:intergenic | 50.0% | |
| CCTGGCCATGTTAGGGATAT+TGG | + | chr2.2:2656232-2656251 | MS.gene36384:CDS | 50.0% | |
| GGTGGAAGGAACTTCAGCAA+AGG | + | chr2.2:2656114-2656133 | MS.gene36384:CDS | 50.0% | |
| TCATGGACCTGGCCATGTTA+GGG | + | chr2.2:2656225-2656244 | MS.gene36384:CDS | 50.0% | |
| CTAACATGGCCAGGTCCATG+AGG | - | chr2.2:2656226-2656245 | None:intergenic | 55.0% | |
| CTCATGGACCTGGCCATGTT+AGG | + | chr2.2:2656224-2656243 | MS.gene36384:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.2 | gene | 2656022 | 2656315 | 2656022 | ID=MS.gene36384 |
| chr2.2 | mRNA | 2656022 | 2656315 | 2656022 | ID=MS.gene36384.t1;Parent=MS.gene36384 |
| chr2.2 | exon | 2656022 | 2656315 | 2656022 | ID=MS.gene36384.t1.exon1;Parent=MS.gene36384.t1 |
| chr2.2 | CDS | 2656022 | 2656315 | 2656022 | ID=cds.MS.gene36384.t1;Parent=MS.gene36384.t1 |
| Gene Sequence |
| Protein sequence |
