AlfalfaGEDB | MS.gene40751

M. sativa cultivar XinJiangDaYe / MS.gene40751

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40751.t1	AES61490.1	88.9	108	8	3	1	106	1	106	4.80E-42	180.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40751.t1	G7IAA9	88.9	108	8	3	1	106	1	106	3.5e-42	180.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049150	MS.gene40751	0.802068	6.68E-49	-1.69E-46
MS.gene049260	MS.gene40751	0.833856	4.19E-56	-1.69E-46
MS.gene049346	MS.gene40751	0.806125	9.57E-50	-1.69E-46
MS.gene049351	MS.gene40751	0.802527	5.38E-49	-1.69E-46
MS.gene049681	MS.gene40751	0.802844	4.63E-49	-1.69E-46
MS.gene049758	MS.gene40751	0.835329	1.79E-56	-1.69E-46
MS.gene050128	MS.gene40751	0.831899	1.28E-55	-1.69E-46
MS.gene050301	MS.gene40751	0.861918	7.32E-64	-1.69E-46
MS.gene050516	MS.gene40751	0.803146	4.01E-49	-1.69E-46
MS.gene050558	MS.gene40751	0.817252	3.63E-52	-1.69E-46
MS.gene050650	MS.gene40751	0.80353	3.34E-49	-1.69E-46
MS.gene05101	MS.gene40751	0.802114	6.54E-49	-1.69E-46
MS.gene051487	MS.gene40751	0.820858	5.49E-53	-1.69E-46
MS.gene051789	MS.gene40751	0.80217	6.37E-49	-1.69E-46
MS.gene051860	MS.gene40751	0.852612	4.12E-61	-1.69E-46
MS.gene052011	MS.gene40751	-0.807173	5.75E-50	-1.69E-46
MS.gene052078	MS.gene40751	0.803995	2.67E-49	-1.69E-46
MS.gene052178	MS.gene40751	0.84552	3.86E-59	-1.69E-46
MS.gene052207	MS.gene40751	0.818105	2.33E-52	-1.69E-46
MS.gene052253	MS.gene40751	0.825962	3.52E-54	-1.69E-46
MS.gene052917	MS.gene40751	-0.804173	2.45E-49	-1.69E-46
MS.gene053074	MS.gene40751	0.828268	9.88E-55	-1.69E-46
MS.gene053204	MS.gene40751	0.818708	1.70E-52	-1.69E-46
MS.gene053836	MS.gene40751	0.846863	1.66E-59	-1.69E-46
MS.gene054010	MS.gene40751	0.897299	1.61E-76	-1.69E-46
MS.gene054011	MS.gene40751	0.905008	6.76E-80	-1.69E-46
MS.gene054012	MS.gene40751	0.899053	2.90E-77	-1.69E-46
MS.gene054013	MS.gene40751	0.898166	6.92E-77	-1.69E-46
MS.gene054183	MS.gene40751	0.815885	7.35E-52	-1.69E-46
MS.gene054184	MS.gene40751	0.802929	4.44E-49	-1.69E-46
MS.gene054185	MS.gene40751	0.812109	5.00E-51	-1.69E-46
MS.gene054214	MS.gene40751	0.833221	6.04E-56	-1.69E-46
MS.gene054473	MS.gene40751	0.83335	5.61E-56	-1.69E-46
MS.gene054596	MS.gene40751	0.80156	8.50E-49	-1.69E-46
MS.gene054618	MS.gene40751	0.828361	9.39E-55	-1.69E-46
MS.gene055277	MS.gene40751	0.81902	1.45E-52	-1.69E-46
MS.gene055303	MS.gene40751	0.807403	5.14E-50	-1.69E-46
MS.gene055390	MS.gene40751	0.818032	2.42E-52	-1.69E-46
MS.gene055422	MS.gene40751	0.842551	2.41E-58	-1.69E-46
MS.gene055428	MS.gene40751	0.832924	7.16E-56	-1.69E-46
MS.gene055604	MS.gene40751	0.867957	9.33E-66	-1.69E-46
MS.gene056191	MS.gene40751	0.842433	2.59E-58	-1.69E-46
MS.gene056902	MS.gene40751	0.855759	5.08E-62	-1.69E-46
MS.gene056931	MS.gene40751	0.804926	1.71E-49	-1.69E-46
MS.gene056933	MS.gene40751	0.800714	1.27E-48	-1.69E-46
MS.gene05702	MS.gene40751	0.807194	5.69E-50	-1.69E-46
MS.gene057121	MS.gene40751	0.831659	1.47E-55	-1.69E-46
MS.gene057245	MS.gene40751	0.822283	2.57E-53	-1.69E-46
MS.gene057469	MS.gene40751	0.834879	2.32E-56	-1.69E-46
MS.gene057752	MS.gene40751	0.804116	2.52E-49	-1.69E-46
MS.gene057779	MS.gene40751	0.80363	3.18E-49	-1.69E-46
MS.gene058002	MS.gene40751	0.830925	2.23E-55	-1.69E-46
MS.gene05811	MS.gene40751	0.858266	9.27E-63	-1.69E-46
MS.gene058168	MS.gene40751	0.803475	3.43E-49	-1.69E-46
MS.gene058484	MS.gene40751	0.816601	5.08E-52	-1.69E-46
MS.gene058660	MS.gene40751	0.804155	2.47E-49	-1.69E-46
MS.gene059006	MS.gene40751	0.802811	4.70E-49	-1.69E-46
MS.gene05907	MS.gene40751	0.844631	6.71E-59	-1.69E-46
MS.gene059228	MS.gene40751	0.814647	1.38E-51	-1.69E-46
MS.gene059294	MS.gene40751	0.85896	5.75E-63	-1.69E-46
MS.gene059518	MS.gene40751	0.84451	7.23E-59	-1.69E-46
MS.gene05984	MS.gene40751	0.839612	1.43E-57	-1.69E-46
MS.gene059974	MS.gene40751	0.810211	1.29E-50	-1.69E-46
MS.gene060005	MS.gene40751	0.810269	1.25E-50	-1.69E-46
MS.gene06037	MS.gene40751	0.8479	8.63E-60	-1.69E-46
MS.gene060559	MS.gene40751	0.806698	7.25E-50	-1.69E-46
MS.gene061049	MS.gene40751	0.84712	1.41E-59	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40751.t1	MTR_1g084780	86.111	108	11	3	1	106	1	106	2.19e-49	152

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 44 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTCCCATGTTGAAAGTTTC+AGG	0.145284	1.2:+59040636	None:intergenic
ACTATGGTCCTGATGGTTTA+AGG	0.152607	1.2:-59040563	MS.gene40751:CDS
CCTGGGACTCTTGGTGGAAT+TGG	0.298107	1.2:-59040489	MS.gene40751:CDS
CCTACTATCCCTGGGACTCT+TGG	0.340847	1.2:-59040498	MS.gene40751:CDS
TGCATTATGTTGGTTGTCCT+TGG	0.369781	1.2:-59040714	MS.gene40751:CDS
GGTGGAATTGGAAATGGAAT+TGG	0.405856	1.2:-59040477	MS.gene40751:CDS
AGCAAAGTTACCAGGGCTGC+TGG	0.427625	1.2:+59040596	None:intergenic
ACTCTTGGTGGAATTGGAAA+TGG	0.433410	1.2:-59040483	MS.gene40751:CDS
TTCAACATGGGAGGAGGGAA+TGG	0.438827	1.2:-59040627	MS.gene40751:CDS
CCAAGAGTCCCAGGGATAGT+AGG	0.443589	1.2:+59040498	None:intergenic
ACTATCCCTGGGACTCTTGG+TGG	0.449895	1.2:-59040495	MS.gene40751:CDS
GGTGATGTTGTGCATTATGT+TGG	0.453491	1.2:-59040724	MS.gene40751:CDS
ACACCTGCAGCAAAGTTACC+AGG	0.471040	1.2:+59040588	None:intergenic
AGCCCTGGTAACTTTGCTGC+AGG	0.474026	1.2:-59040591	MS.gene40751:CDS
CCAATTCCACCAAGAGTCCC+AGG	0.481288	1.2:+59040489	None:intergenic
TTTGCTGCAGGTGTAAACTA+TGG	0.486819	1.2:-59040579	MS.gene40751:CDS
TCAGTTCATGTGGAAGGAGC+TGG	0.487807	1.2:-59040690	MS.gene40751:CDS
TTTGTTGTATTCTATGGATG+TGG	0.489015	1.2:+59040438	None:intergenic
TTCCCTGGTGGCTGTACCAG+TGG	0.504867	1.2:-59040531	MS.gene40751:CDS
ATTGGAAATGGAATTGGAGC+TGG	0.505279	1.2:-59040471	MS.gene40751:CDS
TCAACATGGGAGGAGGGAAT+GGG	0.507559	1.2:-59040626	MS.gene40751:CDS
TTGCCACTGGTACAGCCACC+AGG	0.516455	1.2:+59040528	None:intergenic
CTTGGTTCAGTTCATGTGGA+AGG	0.517140	1.2:-59040696	MS.gene40751:CDS
GAAACTTTCAACATGGGAGG+AGG	0.522497	1.2:-59040633	MS.gene40751:CDS
GTGCAAAACCTTAAACCATC+AGG	0.527769	1.2:+59040555	None:intergenic
GTTGTATTCTATGGATGTGG+TGG	0.547296	1.2:+59040441	None:intergenic
GGGATACTTCCCAGCAGCCC+TGG	0.547402	1.2:-59040606	MS.gene40751:CDS
GCAAAGTTACCAGGGCTGCT+GGG	0.564631	1.2:+59040597	None:intergenic
AGCTGGAAGGTTGCTGAAGG+AGG	0.566335	1.2:-59040673	MS.gene40751:CDS
ACTGGTACAGCCACCAGGGA+AGG	0.569138	1.2:+59040533	None:intergenic
TGTCCTTGGTTCAGTTCATG+TGG	0.588978	1.2:-59040700	MS.gene40751:CDS
GGTGAGAGTTGCAAAGGTGT+TGG	0.597999	1.2:-59040745	MS.gene40751:CDS
ACATCCTGAAACTTTCAACA+TGG	0.599933	1.2:-59040640	MS.gene40751:CDS
AAACTTTCAACATGGGAGGA+GGG	0.601619	1.2:-59040632	MS.gene40751:CDS
AGTAGGAAAAGTATTGCCAC+TGG	0.619129	1.2:+59040515	None:intergenic
GGTGTAAACTATGGTCCTGA+TGG	0.623829	1.2:-59040570	MS.gene40751:CDS
CATCCTGAAACTTTCAACAT+GGG	0.624107	1.2:-59040639	MS.gene40751:CDS
AGGAGCTGGAAGGTTGCTGA+AGG	0.626981	1.2:-59040676	MS.gene40751:CDS
CAATTCCACCAAGAGTCCCA+GGG	0.630518	1.2:+59040490	None:intergenic
CACCTGCAGCAAAGTTACCA+GGG	0.639196	1.2:+59040589	None:intergenic
CCTGAAACTTTCAACATGGG+AGG	0.659348	1.2:-59040636	MS.gene40751:CDS
CTTCCACATGAACTGAACCA+AGG	0.677096	1.2:+59040697	None:intergenic
TGCCACTGGTACAGCCACCA+GGG	0.681445	1.2:+59040529	None:intergenic
TTCATGTGGAAGGAGCTGGA+AGG	0.707170	1.2:-59040686	MS.gene40751:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACATCCTGAAACTTTCAACA+TGG	-	chr1.2:59040556-59040575	MS.gene40751:CDS	35.0%
	CATCCTGAAACTTTCAACAT+GGG	-	chr1.2:59040557-59040576	MS.gene40751:CDS	35.0%
!	AATACTTTTCCTACTATCCC+TGG	-	chr1.2:59040689-59040708	MS.gene40751:CDS	35.0%
!	ATACTTTTCCTACTATCCCT+GGG	-	chr1.2:59040690-59040709	MS.gene40751:CDS	35.0%
	AAACTTTCAACATGGGAGGA+GGG	-	chr1.2:59040564-59040583	MS.gene40751:CDS	40.0%
	ACTATGGTCCTGATGGTTTA+AGG	-	chr1.2:59040633-59040652	MS.gene40751:CDS	40.0%
	ACTCTTGGTGGAATTGGAAA+TGG	-	chr1.2:59040713-59040732	MS.gene40751:CDS	40.0%
	ATTGGAAATGGAATTGGAGC+TGG	-	chr1.2:59040725-59040744	MS.gene40751:CDS	40.0%
	GGTGATGTTGTGCATTATGT+TGG	-	chr1.2:59040472-59040491	MS.gene40751:CDS	40.0%
	GGTGGAATTGGAAATGGAAT+TGG	-	chr1.2:59040719-59040738	MS.gene40751:CDS	40.0%
	GTGCAAAACCTTAAACCATC+AGG	+	chr1.2:59040644-59040663	None:intergenic	40.0%
	TTTGCTGCAGGTGTAAACTA+TGG	-	chr1.2:59040617-59040636	MS.gene40751:CDS	40.0%
!	TGCATTATGTTGGTTGTCCT+TGG	-	chr1.2:59040482-59040501	MS.gene40751:CDS	40.0%
!!	AGTAGGAAAAGTATTGCCAC+TGG	+	chr1.2:59040684-59040703	None:intergenic	40.0%
	CCTCCCATGTTGAAAGTTTC+AGG	+	chr1.2:59040563-59040582	None:intergenic	45.0%
	CCTGAAACTTTCAACATGGG+AGG	-	chr1.2:59040560-59040579	MS.gene40751:CDS	45.0%
	CTTCCACATGAACTGAACCA+AGG	+	chr1.2:59040502-59040521	None:intergenic	45.0%
	CTTGGTTCAGTTCATGTGGA+AGG	-	chr1.2:59040500-59040519	MS.gene40751:CDS	45.0%
	GAAACTTTCAACATGGGAGG+AGG	-	chr1.2:59040563-59040582	MS.gene40751:CDS	45.0%
	GGTGTAAACTATGGTCCTGA+TGG	-	chr1.2:59040626-59040645	MS.gene40751:CDS	45.0%
	TGTCCTTGGTTCAGTTCATG+TGG	-	chr1.2:59040496-59040515	MS.gene40751:CDS	45.0%
!	TTAAGGTTTTGCACCTTCCC+TGG	-	chr1.2:59040650-59040669	MS.gene40751:CDS	45.0%
	ACACCTGCAGCAAAGTTACC+AGG	+	chr1.2:59040611-59040630	None:intergenic	50.0%
	CAATTCCACCAAGAGTCCCA+GGG	+	chr1.2:59040709-59040728	None:intergenic	50.0%
	CACCTGCAGCAAAGTTACCA+GGG	+	chr1.2:59040610-59040629	None:intergenic	50.0%
	TCAACATGGGAGGAGGGAAT+GGG	-	chr1.2:59040570-59040589	MS.gene40751:CDS	50.0%
	TCAGTTCATGTGGAAGGAGC+TGG	-	chr1.2:59040506-59040525	MS.gene40751:CDS	50.0%
	TTCAACATGGGAGGAGGGAA+TGG	-	chr1.2:59040569-59040588	MS.gene40751:CDS	50.0%
	TTCATGTGGAAGGAGCTGGA+AGG	-	chr1.2:59040510-59040529	MS.gene40751:CDS	50.0%
!	GGTGAGAGTTGCAAAGGTGT+TGG	-	chr1.2:59040451-59040470	MS.gene40751:CDS	50.0%
	ACTATCCCTGGGACTCTTGG+TGG	-	chr1.2:59040701-59040720	MS.gene40751:CDS	55.0%
	AGCAAAGTTACCAGGGCTGC+TGG	+	chr1.2:59040603-59040622	None:intergenic	55.0%
	AGCCCTGGTAACTTTGCTGC+AGG	-	chr1.2:59040605-59040624	MS.gene40751:CDS	55.0%
	AGCTGGAAGGTTGCTGAAGG+AGG	-	chr1.2:59040523-59040542	MS.gene40751:CDS	55.0%
	AGGAGCTGGAAGGTTGCTGA+AGG	-	chr1.2:59040520-59040539	MS.gene40751:CDS	55.0%
	CCAAGAGTCCCAGGGATAGT+AGG	+	chr1.2:59040701-59040720	None:intergenic	55.0%
	CCAATTCCACCAAGAGTCCC+AGG	+	chr1.2:59040710-59040729	None:intergenic	55.0%
	CCTACTATCCCTGGGACTCT+TGG	-	chr1.2:59040698-59040717	MS.gene40751:CDS	55.0%
	CCTGGGACTCTTGGTGGAAT+TGG	-	chr1.2:59040707-59040726	MS.gene40751:CDS	55.0%
	GCAAAGTTACCAGGGCTGCT+GGG	+	chr1.2:59040602-59040621	None:intergenic	55.0%
!	AGGTTTTGCACCTTCCCTGG+TGG	-	chr1.2:59040653-59040672	MS.gene40751:CDS	55.0%
	ACTGGTACAGCCACCAGGGA+AGG	+	chr1.2:59040666-59040685	None:intergenic	60.0%
	TGCCACTGGTACAGCCACCA+GGG	+	chr1.2:59040670-59040689	None:intergenic	60.0%
	TTCCCTGGTGGCTGTACCAG+TGG	-	chr1.2:59040665-59040684	MS.gene40751:CDS	60.0%
	TTGCCACTGGTACAGCCACC+AGG	+	chr1.2:59040671-59040690	None:intergenic	60.0%
	GGGATACTTCCCAGCAGCCC+TGG	-	chr1.2:59040590-59040609	MS.gene40751:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	59040449	59040769	59040449	ID=MS.gene40751
chr1.2	mRNA	59040449	59040769	59040449	ID=MS.gene40751.t1;Parent=MS.gene40751
chr1.2	exon	59040449	59040769	59040449	ID=MS.gene40751.t1.exon1;Parent=MS.gene40751.t1
chr1.2	CDS	59040449	59040769	59040449	ID=cds.MS.gene40751.t1;Parent=MS.gene40751.t1

Gene Sequence	>MS.gene40751 ATGGTGAGAGTTGCAAAGGTGTTGGTGATGTTGTGCATTATGTTGGTTGTCCTTGGTTCAGTTCATGTGGAAGGAGCTGGAAGGTTGCTGAAGGAGGAGAGTGTGAAACATCCTGAAACTTTCAACATGGGAGGAGGGAATGGGATACTTCCCAGCAGCCCTGGTAACTTTGCTGCAGGTGTAAACTATGGTCCTGATGGTTTAAGGTTTTGCACCTTCCCTGGTGGCTGTACCAGTGGCAATACTTTTCCTACTATCCCTGGGACTCTTGGTGGAATTGGAAATGGAATTGGAGCTGGATTCTCACCACCACATCCATAG
Protein sequence	>MS.gene40751.t1 MVRVAKVLVMLCIMLVVLGSVHVEGAGRLLKEESVKHPETFNMGGGNGILPSSPGNFAAGVNYGPDGLRFCTFPGGCTSGNTFPTIPGTLGGIGNGIGAGFSPPHP